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At least 523 records · Page 29

EDGE COVID-19: a web platform to generate submission-ready genomes from SARS-CoV-2 sequencing efforts

Abstract Summary Genomics has become an essential technology for surveilling emerging infectious disease outbreaks. A range of technologies and strategies for pathogen genome enrichment and sequencing are being used by laboratories worldwide, together with different and sometimes ad hoc, analytical procedures for generating genome sequences. A fully integrated analytical process for raw sequence to consensus genome determination, suited to outbreaks such as the ongoing COVID-19 pandemic, is critical to provide a solid genomic basis for epidemiological analyses and well-informed decision making. We have developed a web-based platform and integrated bioinformatic workflows that help to provide consistent high-quality analysis of SARS-CoV-2 sequencing data generated with either the Illumina or Oxford Nanopore Technologies (ONT). Using an intuitive web-based interface, this workflow automates data quality control, SARS-CoV-2 reference-based genome variant and consensus calling, lineage determination and provides the ability to submit the consensus sequence and necessary metadata to GenBank, GISAID and INSDC raw data repositories. We tested workflow usability using real world data and validated the accuracy of variant and lineage analysis using several test datasets, and further performed detailed comparisons with results from the COVID-19 Galaxy Project workflow. Our analyses indicate that EC-19 workflows generate high-quality SARS-CoV-2 genomes. Finally, we share a perspective on patterns and impact observed with Illumina versus ONT technologies on workflow congruence and differences. Availability and implementation https://edge-covid19.edgebioinformatics.org, and https://github.com/LANL-Bioinformatics/EDGE/tree/SARS-CoV2. Supplementary information Supplementary data are available at Bioinformatics online.

59 BASIC BIOLOGICAL SCIENCES↗

Potentially adaptive SARS-CoV-2 mutations discovered with novel spatiotemporal and explainable AI models

Abstract Background A mechanistic understanding of the spread of SARS-CoV-2 and diligent tracking of ongoing mutagenesis are of key importance to plan robust strategies for confining its transmission. Large numbers of available sequences and their dates of transmission provide an unprecedented opportunity to analyze evolutionary adaptation in novel ways. Addition of high-resolution structural information can reveal the functional basis of these processes at the molecular level. Integrated systems biology-directed analyses of these data layers afford valuable insights to build a global understanding of the COVID-19 pandemic. Results Here we identify globally distributed haplotypes from 15,789 SARS-CoV-2 genomes and model their success based on their duration, dispersal, and frequency in the host population. Our models identify mutations that are likely compensatory adaptive changes that allowed for rapid expansion of the virus. Functional predictions from structural analyses indicate that, contrary to previous reports, the Asp 614 Gly mutation in the spike glycoprotein (S) likely reduced transmission and the subsequent Pro 323 Leu mutation in the RNA-dependent RNA polymerase led to the precipitous spread of the virus. Our model also suggests that two mutations in the nsp13 helicase allowed for the adaptation of the virus to the Pacific Northwest of the USA. Finally, our explainable artificial intelligence algorithm identified a mutational hotspot in the sequence of S that also displays a signature of positive selection and may have implications for tissue or cell-specific expression of the virus. Conclusions These results provide valuable insights for the development of drugs and surveillance strategies to combat the current and future pandemics.

59 BASIC BIOLOGICAL SCIENCES↗

Real-time processor for the Danish airborne SAR

A real-time processor for the Danish high-resolution SAR is presented in terms of its functional performance, algorithm, architecture, and implementation. The real-time processor is mainly intended to assist the operator in using the SAR system, but since the processor has been designed to produce high-quality images, it is expected to make off-line processing superfluous in many cases. The range-Doppler algorithm is adopted and supplemented with an extensive motion compensation, considering the special conditions related to the real-time strip mapping of large scenes. The processor is a pipeline of about 20 elements interconnected by a dedicated data path and a control bus. Only three different types of elements are involved: a programmable signal processing element, a multipurpose memory element, and a multipurpose interface element. The prototypes of these three elements have been tested with satisfactory results.

Dall, J.↗

Land Covering Classifications of Boreas Modeling Grid Using AIRSAR Images

Mapping forest types in the boreal ecosystem in an integrated part of any modeling excercise of biogeophysical processes characterizing the interaction of forest with the atmosphere. In this paper, we report the results of the land cover classification of the SAR data acquired during the BOREAS (BOReal Ecosystem Atmospheric Study) intensive field campaigns over the modeling sub-grid of the southern study area in Saskatchewan , Canada. A Bayesian-maximum-a-posteriori classifier has been applied on the NASA/JPL AIRSAR images covering the region during the peak of the growing season in July, 1994.

Atmospheric Study↗

Triangulation using synthetic aperture radar images

For the extraction of topographic information about Venus from stereoradar images obtained from the Magellan Mission, a Synthetic Aperture Radar (SAR) compilation system was developed on analytical stereoplotters. The system software was extensively tested by using stereoradar images from various spacecraft and airborne radar systems, including Seasat, SIR-B, ERIM XCL, and STAR-1. Stereomodeling from radar images was proven feasible, and development is on a correct approach. During testing, the software was enhanced and modified to obtain more flexibility and better precision. Triangulation software for establishing control points by using SAR images was also developed through a joint effort with the Defense Mapping Agency. The SAR triangulation system comprises four main programs, TRIDATA, MODDATA, TRISAR, and SHEAR. The first two programs are used to sort and update the data; the third program, the main one, performs iterative statistical adjustment; and the fourth program analyzes the results. Also, input are flight data and data from the Global Positioning System and Inertial System (navigation information). The SAR triangulation system was tested with six strips of STAR-1 radar images on a VAX-750 computer. Each strip contains images of 10 minutes flight time (equivalent to a ground distance of 73.5 km); the images cover a ground width of 22.5 km. All images were collected from the same side. With an input of 44 primary control points, 441 ground control points were produced. The adjustment process converged after eight iterations. With a 6-m/pixel resolution of the radar images, the triangulation adjustment has an average standard elevation error of 81 m. Development of Magellan radargrammetry will be continued to convert both SAR compilation and triangulation systems into digital form.

Wu, Sherman S. C.↗

Study of Synthetic Aperture Radar (SAR) imagery characteristics

Sources of geometric and radiometric fidelity errors in AN/APQ-102A radar imagery are discussed, along with a digital computer program to correct the distortions. The major effort, a computer program which will process digitalized recorded AN/APQ-102A phase histories into imagery, is described. All computer programs are listed.

Source record↗

Polarization signatures for abandoned agricultural fields in the Manix Basin area of the Mojave Desert - Can polarimetric SAR detect desertification?

Radar backscatter from abandoned circular alfalfa fields in the Manix Basin area of the Mojave desert shows systematic changes with length of abandonment. The obliteration of circular planting rows by surface processes could account for the disappearance of bright spokes, which seem to be reflection patterns from remnants of the planting rows, with increasing length of abandonment. An observed shift in the location of the maximum L-band copolarization return away from VV, as well as an increase in surface roughness, both occurring with increasing age of abandonment, seems to be attributable to the formation of wind ripples on the relatively vegetationless fields.

Ray, Terrill W.↗

Airborne Radar Interferometric Repeat-Pass Processing

Earth science research often requires crustal deformation measurements at a variety of time scales, from seconds to decades. Although satellites have been used for repeat-track interferometric (RTI) synthetic-aperture-radar (SAR) mapping for close to 20 years, RTI is much more difficult to implement from an airborne platform owing to the irregular trajectory of the aircraft compared with microwave imaging radar wavelengths. Two basic requirements for robust airborne repeat-pass radar interferometry include the ability to fly the platform to a desired trajectory within a narrow tube and the ability to have the radar beam pointed in a desired direction to a fraction of a beam width. Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) is equipped with a precision auto pilot developed by NASA Dryden that allows the platform, a Gulfstream III, to nominally fly within a 5 m diameter tube and with an electronically scanned antenna to position the radar beam to a fraction of a beam width based on INU (inertial navigation unit) attitude angle measurements.

Hensley, Scott↗

Azimuth correlator design for IC chip

Azimuth correlator circuit synthetic-aperature radar (SAR) is designed for single integrated circuit (IC) chip. Azimuth correlator modules constructed with sets of such chips could make real-time signal processing possible. Primary advantages are realized in areas of weight and power requirement reductions.

Tyree, V. C.↗

The use of radar and LANDSAT data for mineral and petroleum exploration in the Los Andes region, Venezuela

A geological study of a 27,500 sq km area in the Los Andes region of northwestern Venezuela was performed which employed both X-band radar mosaics and computer processed Landsat images. The 3.12 cm wavelength radar data were collected with horizontal-horizontal polarization and 10 meter spatial resolution by an Aeroservices SAR system at an altitude of 12,000 meters. The radar images increased the number of observable suspected fractures by 27 percent over what could be mapped by LANDSAT alone, owing mostly to the cloud cover penetration capabilities of radar. The approximate eight fold greater spatial resolution of the radar images made possible the identification of shorter, narrower fractures than could be detected with LANDSAT data alone, resulting in the discovery of a low relief anticline that could not be observed in LANDSAT data. Exploration targets for petroleum, copper, and uranium were identified for further geophysical work.

Vincent, R. K.↗

Spaceborne scanner imaging system errors

The individual sensor system design elements which are the priori components in the registration and rectification process, and the potential impact of error budgets on multitemporal registration and side-lap registration are analyzed. The properties of scanner, MLA, and SAR imaging systems are reviewed. Each sensor displays internal distortion properties which to varying degrees make it difficult to generate on orthophoto projection of the data acceptable for multiple pass registration or meeting national map accuracy standards and is also affected to varying degrees by relief displacements in moderate to hilly terrain. Nonsensor related distortions, associated with the accuracy of ephemeris determination and platform stability, have a major impact on local geometric distortions. Platform stability improvements expected from the new multi mission spacecraft series and improved ephemeris and ground control point determination from the NAVSTAR/global positioning satellite systems are reviewed.

Prakash, A.↗

Digital simulation of the SIR-C sensor electronics

In this paper software for simulation of the response of the SIR-C sensor to a point target is described. Synthetic SAR data is generated by passing successive chirps through a simulation of the transmitter electronics, propagation path and receiver electronics. This result is then processed with a digital correlator to yield the point target response of the system. This allows an accurate assessment of the effect of the radar design on the final image product.

Klein, Jeffrey D.↗

Thematic Conference on Remote Sensing for Exploration Geology, 6th, Houston, TX, May 16-19, 1988, Proceedings. Volumes 1 & 2

Papers concerning remote sensing applications for exploration geology are presented, covering topics such as remote sensing technology, data availability, frontier exploration, and exploration in mature basins. Other topics include offshore applications, geobotany, mineral exploration, engineering and environmental applications, image processing, and prospects for future developments in remote sensing for exploration geology. Consideration is given to the use of data from Landsat, MSS, TM, SAR, short wavelength IR, the Geophysical Environmental Research Airborne Scanner, gas chromatography, sonar imaging, the Airborne Visible-IR Imaging Spectrometer, field spectrometry, airborne thermal IR scanners, SPOT, AVHRR, SIR, the Large Format camera, and multitimephase satellite photographs.

Source record↗

Probing the Martian Subsurface with Synthetic Aperture Radar

Many regions of the martian surface are covered by fine-grained materials emplaced by volcanic, fluvial, or aeolian processes. These mantling deposits likely hide ancient channel systems (particularly at smaller scale lengths) and volcanic, impact, glacial, or shoreline features. Synthetic aperture radar (SAR) offers the capability to probe meters below the surface, with imaging resolution in the 10 s of m range, to reveal the buried terrain and enhance our understanding of Mars geologic and climate history. This presentation focuses on the practical applications of a Mars orbital SAR, methods for polarimetric and interferometric radar studies, and examples of such techniques for Mars-analog sites on the Moon and Earth.

Campbell, B. A.↗

A pattern recognition system for locating small volvanoes in Magellan SAR images of Venus

The Magellan data set constitutes an example of the large volumes of data that today's instruments can collect, providing more detail of Venus than was previously available from Pioneer Venus, Venera 15/16, or ground-based radar observations put together. However, data analysis technology has not kept pace with data collection and storage technology. Due to the sheer size of the data, complete and comprehensive scientific analysis of such large volumes of image data is no longer feasible without the use of computational aids. Our progress towards developing a pattern recognition system for aiding in the detection and cataloging of small-scale natural features in large collections of images is reported. Combining classical image processing, machine learning, and a graphical user interface, the detection of the 'small-shield' volcanoes (less than 15km in diameter) that constitute the most abundant visible geologic feature in the more that 30,000 synthetic aperture radar (SAR) images of the surface of Venus are initially targeted. Our eventual goal is to provide a general, trainable tool for locating small-scale features where scientists specify what to look for simply by providing examples and attributes of interest to measure. This contrasts with the traditional approach of developing problem specific programs for detecting Specific patterns. The approach and initial results in the specific context of locating small volcanoes is reported. It is estimated, based on extrapolating from previous studies and knowledge of the underlying geologic processes, that there should be on the order of 10(exp 5) to 10(exp 6) of these volcanoes visible in the Magellan data. Identifying and studying these volcanoes is fundamental to a proper understanding of the geologic evolution of Venus. However, locating and parameterizing them in a manual manner is forbiddingly time-consuming. Hence, the development of techniques to partially automate this task were undertaken. The primary constraints for this particular problem are that the method must be reasonably robust and fast. Unlike most geological features, the small volcanoes of Venus can be ascribed to a basic process that produces features with a short list of readily defined characteristics differing significantly from other surface features on Venus. For pattern recognition purposes the relevant criteria include (1) a circular planimetric outline, (2) known diameter frequency distribution from preliminary studies, (3) a limited number of basic morphological shapes, and (4) the common occurrence of a single, circular summit pit at the center of the edifice.

Burl, M. C.↗

Waves and mesoscale features in the marginal ice zone

Ocean-ice interaction processes in the Marginal Ice Zone (MIZ) by waves and mesoscale features, such as upwelling and eddies, are studied using ERS-1 Synthetic Aperture Radar (SAR) imagery and wave-ice interaction models. Satellite observations of mesoscale features can play a crucial role in ocean-ice interaction study.

Liu, Antony K.↗

Multifrequency, Multipolarization External Calibration of the SIR-C/X-SAR Radars

Progress and the future plans for the following objectives are discussed: (1) Assess the accuracy at which the SIR-C/X-SAR standard data products can be calibrated through the use of ground calibrators to estimate the end-to-end system polarization calibration constants (or distortion parameters) and incorporate the constants into the data processing; (2) Study the cross-calibration between three multipolarization systems: SIR-C, the National Aeronautics and Space Administration/Jet Propulsion Laboratory (NASA/JPL) DC-8 SAR, and the University of Michigan ground-based polarimetric scatterometer; (3) Evaluate the calibration "stability" of SIR-C/X-SAR (measured by variations in the calibration constants) over the range swath width and over a specified distance in azimuth. Variations over a 12-hour period (between ascending and descending passes) will also be studied; and (4) Develop a cost-effective calibration plan including development of inexpensive polarimetric active calibrators.

Freeman, Anthony↗

Models and Processes to Extract Drug-like Molecules From Natural Language Text

Researchers worldwide are seeking to repurpose existing drugs or discover new drugs to counter the disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A promising source of candidates for such studies is molecules that have been reported in the scientific literature to be drug-like in the context of viral research. However, this literature is too large for human review and features unusual vocabularies for which existing named entity recognition (NER) models are ineffective. We report here on a project that leverages both human and artificial intelligence to detect references to such molecules in free text. We present 1) a iterative model-in-the-loop method that makes judicious use of scarce human expertise in generating training data for a NER model, and 2) the application and evaluation of this method to the problem of identifying drug-like molecules in the COVID-19 Open Research Dataset Challenge (CORD-19) corpus of 198,875 papers. We show that by repeatedly presenting human labelers only with samples for which an evolving NER model is uncertain, our human-machine hybrid pipeline requires only modest amounts of non-expert human labeling time (tens of hours to label 1778 samples) to generate an NER model with an F-1 score of 80.5%—on par with that of non-expert humans—and when applied to CORD’19, identifies 10,912 putative drug-like molecules. This enriched the computational screening team’s targets by 3,591 molecules, of which 18 ranked in the top 0.1% of all 6.6 million molecules screened for docking against the 3CLPro protein.

60 APPLIED LIFE SCIENCES↗