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DOE OSTI2023

Optimization of peptide amphiphile-lipid raft interaction by changing peptide amphiphile lipophilicity

Various peptide amphiphile (PA) molecules have been developed to promote bone regeneration. Previously we discovered that a peptide amphiphile with a palmitic acid tail (C 16 ) attenuates the signaling threshold of leucine-rich amelogenin peptide (LRAP)-mediated Wnt activation by increasing membrane lipid raft mobility. In the current study, we found that treatment of murine ST2 cells with an inhibitor (Nystatin) or Caveolin-1-specific siRNA abolishes the effect of C 16 PA, indicating that Caveolin-mediated endocytosis is required. Here, to determine whether hydrophobicity of the PA tail plays a role in its signaling effect, we modified the length of the tail (C 12 , C 16 and C 22 ) or composition (cholesterol). While shortening the tail (C 12 ) decreased the signaling effect, lengthening the tail (C 22 ) had no prominent effect. On the other hand, the cholesterol PA displayed a similar function as the C 16 PA at the same concentration of 0.001% w/v. Interestingly, a higher concentration of C 16 PA (0.005%) is cytotoxic while cholesterol PA at the higher concentration (0.005%) is well-tolerated by cells. Use of the cholesterol PA at 0.005% enabled a further reduction of the signaling threshold of LRAP to 0.20 nM, compared to 0.25 nM at 0.001%. Caveolin-mediated endocytosis is also required for cholesterol PA, as evidenced by Caveolin-1 siRNA knockdown experiments. We further demonstrated that the noted effects of cholesterol PA are also observed in human bone marrow mesenchymal stem cells (BMMSCs). Taken together, these results indicate that the cholesterol PA modulates lipid raft/caveolar dynamics, thereby increasing receptor sensitivity for activation of canonical Wnt signaling.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1999

Ethylene plays multiple nonprimary roles in modulating the gravitropic response in tomato

Ethylene is known to interact with auxin in regulating stem growth, and yet evidence for the role of ethylene in tropic responses is contradictory. Our analysis of four mutants of tomato (Lycopersicon esculentum) altered in their response to gravity, auxin, and/or ethylene revealed concentration-dependent modulation of shoot gravitropism by ethylene. Ethylene inhibitors reduce wild-type gravicurvature, and extremely low (0.0005-0.001 microliter L-1) ethylene concentrations can restore the reduced gravitropic response of the auxin-resistant dgt (diageotropica) mutant to wild-type levels. Slightly higher concentrations of ethylene inhibit the gravitropic response of all but the ethylene-insensitive nr (never-ripe) mutant. The gravitropic responses of nr and the constitutive-response mutant epi (epinastic) are slightly and significantly delayed, respectively, but otherwise normal. The reversal of shoot gravicurvature by red light in the lz-2 (lazy-2) mutant is not affected by ethylene. Taken together, these data indicate that, although ethylene does not play a primary role in the gravitropic response of tomato, low levels of ethylene are necessary for a full gravitropic response, and moderate levels of the hormone specifically inhibit gravicurvature in a manner different from ethylene inhibition of overall growth.

Non-NASA Center↗

DOE OSTI2024

FEW questions, many answers: using machine learning to assess how students connect food–energy–water (FEW) concepts

There is growing support and interest in postsecondary interdisciplinary environmental education which integrate concepts and disciplines in addition to providing varied perspectives. There is a need to assess student learning in these programs as well as rigorous evaluation of educational practices, especially of complex synthesis concepts. This work tests a text classification machine learning model as a tool to assess student systems thinking capabilities using two questions anchored by the Food-Energy-Water (FEW) Nexus phenomena by answering two questions (1) Can machine learning models be used to identify instructor-determined important concepts in student responses? (2) What do college students know about the interconnections between food, energy and water, and how have students assimilated systems thinking into their constructed responses about FEW? Reported here are a broad range of model performances across 26 text classification models associated with two different assessment items, with model accuracy ranging from 0.755 to 0.992. Expert-like responses were infrequent in our dataset compared to responses providing simpler, incomplete explanations of the systems presented in the question. For those students moving from describing individual effects to multiple effects, their reasoning about the mechanism behind the system indicates advanced systems thinking ability. Specifically, students exhibit higher expertise for explaining changing water usage than discussing tradeoffs for such changing usage. This research represents one of the first attempts to assess the links between foundational, discipline-specific concepts and systems thinking ability. These text classification approaches to scoring student FEW Nexus Constructed Responses (CR) indicate how these approaches can be used, in addition to several future research priorities for interdisciplinary, practice-based education research. Development of further complex question items using machine learning would allow evaluation of the relationship between foundational concept understanding and integration of those concepts as well as more nuanced understanding of student comprehension of complex interdisciplinary concepts.

96 KNOWLEDGE MANAGEMENT AND PRESERVATION↗

NASA NTRS2011

Wide-Field Optic for Autonomous Acquisition of Laser Link

An innovation reported in Two-Camera Acquisition and Tracking of a Flying Target, NASA Tech Briefs, Vol. 32, No. 8 (August 2008), p. 20, used a commercial fish-eye lens and an electronic imaging camera for initially locating objects with subsequent handover to an actuated narrow-field camera. But this operated against a dark-sky background. An improved solution involves an optical design based on custom optical components for the wide-field optical system that directly addresses the key limitations in acquiring a laser signal from a moving source such as an aircraft or a spacecraft. The first challenge was to increase the light collection entrance aperture diameter, which was approximately 1 mm in the first prototype. The new design presented here increases this entrance aperture diameter to 4.2 mm, which is equivalent to a more than 16 times larger collection area. One of the trades made in realizing this improvement was to restrict the field-of-view to +80 deg. elevation and 360 azimuth. This trade stems from practical considerations where laser beam propagation over the excessively high air mass, which is in the line of sight (LOS) at low elevation angles, results in vulnerability to severe atmospheric turbulence and attenuation. An additional benefit of the new design is that the large entrance aperture is maintained even at large off-axis angles when the optic is pointed at zenith. The second critical limitation for implementing spectral filtering in the design was tackled by collimating the light prior to focusing it onto the focal plane. This allows the placement of the narrow spectral filter in the collimated portion of the beam. For the narrow band spectral filter to function properly, it is necessary to adequately control the range of incident angles at which received light intercepts the filter. When this angle is restricted via collimation, narrower spectral filtering can be implemented. The collimated beam (and the filter) must be relatively large to reduce the incident angle down to only a few degrees. In the presented embodiment, the filter diameter is more than ten times larger than the entrance aperture. Specifically, the filter has a clear aperture of about 51 mm. The optical design is refractive, and is comprised of nine custom refractive elements and an interference filter. The restricted maximum angle through the narrow-band filter ensures the efficient use of a 2-nm noise equivalent bandwidth spectral width optical filter at low elevation angles (where the range is longest), at the expense of less efficiency for high elevations, which can be tolerated because the range at high elevation angles is shorter. The image circle is 12 mm in diameter, mapped to 80 x 360 of sky, centered on the zenith.

Page, Norman A.↗

DOE OSTI2023

Quantifying uncertainties due to irreducible three-body forces in deuteron-nucleus reactions

Deuteron-induced nuclear reactions are an essential tool for probing the structure of nuclei as well as astrophysical information such as (n, γ) cross sections. The deuteron-nucleus system is typically described within a Faddeev three-body model consisting of a neutron (n), a proton (p), and the target nucleus (A) interacting through pairwise phenomenological potentials. While Faddeev techniques enable the exact description of the three-body dynamics, their predictive power is limited in part by the omission of irreducible neutron-proton-nucleus three-body force (n–p–A 3BF). Here, our goal is to quantify systematic uncertainties stemming from the reduction of deuteron-nucleus (d + A) dynamics to a picture of three pointlike nuclear clusters interacting via pairwise nucleon-nucleus forces, using as testing grounds d + α scattering and the 6 Li ground state. We particularly focus on quantifying uncertainties arising from the full antisymmetrization of the (A + 2)-body system with the target nucleus fixed in its ground state. We adopt the ab initio no-core shell model coupled with the resonating group method (NCSM/RGM) to compute microscopic n–α and p–α interactions, and use them in a three-body description of the d + α system by means of momentum-space Faddeev-type equations. Simultaneously, we also carry out ab initio calculations of d + α scattering and 6 Li ground state by means of six-body NCSM/RGM calculations to serve as a benchmark for the three-body model predictions given by the Faddeev calculations. By comparing the Faddeev and NCSM/RGM results, we show that the irreducible n–p–α 3BF has a non-negligible effect on bound state and scattering observables alike. Specifically, the Faddeev approach yields a 6 Li ground state that is approximately 600 keV shallower than the one obtained with the NCSM/RGM. Additionally, the Faddeev calculations for d + α scattering yield a 3 + resonance that is located approximately 400 keV higher in energy compared to the NCSM/RGM result. The shape of the d + α angular distributions computed using the two approaches also differ, owing to the discrepancy in the predictions of the 3 + resonance energy. The Faddeev three-body model predictions for d + α scattering and 6 Li using microscopic n–α and p–α potentials differ from those computed microscopically with the NCSM/RGM. These discrepancies are due to the n–p–α 3BF, which arises from two-nucleon exchange terms in the microscopic d–α interaction and are not accounted for in the three-body model Faddeev calculations. This study lays the foundation for future parametrizations of the 3BF due to Pauli exclusion principle effects in improved three-body calculations of deuteron-induced reactions.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

DOE OSTI2025

PQML: Enabling the Predictive Reproducibility on NISQ Machines for Quantum ML Applications

Quantum computing represents a groundbreaking approach to high-performance computing. In recent years, quantum computers have progressed from single-qubit processors to systems boasting over 400 qubits. The presence of such a large number of qubits offers significant advantages, including enhanced computational speed—a capability beyond classical computing methods. However, the current stage of quantum computing is referred to as the noisy intermediate-scale quantum (NISQ) era. The existence of noise in this era presents challenges in testing quantum computing applications, leading to considerable variance in application results. Furthermore, the diverse noise characteristics observed across different machines exacerbate this issue, complicating the selection of the appropriate machine for application execution. In response to these challenges, we introduce our Predictive Quantum Machine Learning (PQML) tool. This tool is designed to predict outcomes when executing identical quantum machine learning applications—specifically, a critical suite of variational quantum algorithms—across various quantum computers during the NISQ era. This effort relies on data collected over a 12-month period. To the best of our knowledge, this study represents the first attempt to ensure reproducibility across quantum computers for complex circuits. Additionally, we have developed a model capable of forecasting the accuracy of quantum computers for variational quantum algorithms, with a particular emphasis on quantum machine learning as a case study.

Senapati, Priyabrata [Kent State University]↗

DOE OSTI2025

Data for: Climatic Imprint on Interfacially-Controlled Platinum-Palladium Resources

Data package for manuscript "Climatic Imprint on Interfacially-Controlled Platinum-Palladium Resources" by Emily G. Wright, Ivey Wang, Yihang Fang, Elaine D. Flynn, and Jeffrey G. Catalano. This dataset contains adsorption results from experiments designed to investigate the effect of chloride on Pd(II) adsorption to goethite and Pt(II) adsorption to hematite and goethite, including lab experiments, X-ray absorption fine structure spectroscopy, and models of retention within a laterite. See the associated manuscript for full methods information. The file "Wright2025_PtAds_data.csv" contains the target starting Pt concentration (uM), final aqueous Pt and associated error (in uM), calculated adsorbed Pt and associated error (in umol/m2), target and measured aqueous chloride (mM), target aqueous nitrate (mM), final pH, and mineral concentration/loading (g/L). Associated mineral-free controls (mineral loading = 0 g/L) are included; the aqueous Pd error was not calculated and chloride was not measured in every sample. These data appear in Figures 1, S3, S4, S5, S20, and S22 in the associated manuscript. The file "Wright2025_PdAds_data.csv" contains the target starting Pd concentration (uM), final aqueous Pd and associated error (in uM), calculated adsorbed Pd and associated error (in umol/m2), target and measured aqueous chloride (mM), and mineral concentration/loading (g/L). Associated mineral-free controls (mineral loading = 0 g/L) are included; the aqueous Pd error was not calculated and chloride was not measured in every sample. These data appear in Figures 1, S3, S4, S5, and S20 in the associated manuscript. The file "Wright2025_MineralBatches_data.csv" contains the mineral identity and BET specific surface area (m2/g) for every mineral batch synthesized and used in experiments. The annealing time used is listed for hydrothermally annealed goethite. These data appear in Table S2 in the associated manuscript. The file "Wright2025_XRD_data.csv" contains the XRD patterns for every mineral batch synthesized as the counts as a function of two theta (in degrees). See "Wright2025_MineralBatches_data.csv" for more details on specific mineral batches. These data appear in Figure S2 in the associated manuscript. The file "Wright 2025_ZetaPotential_data.csv" contains the measured zeta potentials for samples of goethite (batch G2) at pH 4 the presence of varying amounts of sodium chloride. These data appear in Table S3 in the associated manuscript. The file "Wright2025_XAFSSamples_data.csv" contains the specific mineral batch, measured final aqueous Pd or Pt (uM), measured final aqueous chloride (mM), and estimated adsorbed Pd or Pt (umol/m2) of all XAFS samples. These data appear in Tables S4, S7, S8, and S10 in the associated manuscript. The files "Wright2025_PdXAFS_data.csv" and "Wright2025_PtXAFS_data.csv" contain the normalized spectra of Pd and Pt, respectively, adsorbed to minerals at varying chloride concentrations. See "Wright2025_XAFSSamples_data.csv" for a guide to sample names. Note that "05" in a sample name is equivalent to "0.5". These data appear in Figures 2, S6, S7, S8, S12, S13, and S14 in the associated manuscript. The file "Wright2025_LateriteProfileProfileModelParameters_data.csv" include the ratio of hematite to hematite and goethite in two synthetic, modeled profiles, as well as the modeled surface areas of goethite and hematite as a function of relative depth within the modeled weathering zone. These data were used, in conjunction with equations presented in the paper, to calculate the theoretical concentrations of Pd and Pt (and the resulting Pt/Pd ratio) within the profiles. These data appear in Figure 3 in the associated manuscript. The file "Wright2025_Imagery_data.zip" is a zipped folder containing the TEM and STEM images appear in Figures S18 and S19. Individual files are labeled as either STEM (Fig. S18) or TEM (Fig. S19) with a letter representing the part of the multipart figure.

58 GEOSCIENCES↗

NASA NTRS2018

Comparisons of Bispectral and Polarimetric Retrievals of Marine Boundary Layer Cloud Microphysics: Case Studies Using a LES- Satellite Retrieval Simulator

Many passive remote-sensing techniques have been developed to retrieve cloud microphysical properties from satellite-based sensors, with the most common approaches being the bispectral and polarimetric techniques. These two vastly different retrieval techniques have been implemented for a variety of polar-orbiting and geostationary satellite platforms, providing global climatological data sets. Prior instrument comparison studies have shown that there are systematic differences between the droplet size retrieval products (effective radius) of bispectral (e.g., MODIS, Moderate Resolution Imaging Spectroradiometer) and polarimetric (e.g., POLDER, Polarization and Directionality of Earth's Reflectances) instruments. However, intercomparisons of airborne bispectral and polarimetric instruments have yielded results that do not appear to be systematically biased relative to one another. Diagnosing this discrepancy is complicated, because it is often difficult for instrument intercomparison studies to isolate differences between retrieval technique sensitivities and specific instrumental differences such as calibration and atmospheric correction. In addition to these technical differences the polarimetric retrieval is also sensitive to the dispersion of the droplet size distribution (effective variance), which could influence the interpretation of the droplet size retrieval. To avoid these instrument-dependent complications, this study makes use of a cloud remote-sensing retrieval simulator. Created by coupling a large-eddy simulation (LES) cloud model with a 1-D radiative transfer model, the simulator serves as a test bed for understanding differences between bispectral and polarimetric retrievals. With the help of this simulator we can not only compare the two techniques to one another (retrieval intercomparison) but also validate retrievals directly against the LES cloud properties. Using the satellite retrieval simulator, we are able to verify that at high spatial resolution (50m) the bispectral and polarimetric retrievals are highly correlated with one another within expected observational uncertainties. The relatively small systematic biases at high spatial resolution can be attributed to different sensitivity limitations of the two retrievals. In contrast, a systematic difference between the two retrievals emerges at coarser resolution. This bias largely stems from differences related to sensitivity of the two retrievals to unresolved inhomogeneities in effective variance and optical thickness. The influence of coarse angular resolution is found to increase uncertainty in the polarimetric retrieval but generally maintains a constant mean value.

Miller, Daniel J.↗

NASA NTRS2018

Comparisons of Bispectral and Polarimetric Retrievals of Marine Boundary Layer Cloud Microphysics: Case Studies Using a LES-Satellite Retrieval Simulator

Many passive remote-sensing techniques have been developed to retrieve cloud microphysical properties from satellite-based sensors, with the most common approaches being the bispectral and polarimetric techniques. These two vastly different retrieval techniques have been implemented for a variety of polar-orbiting and geostationary satellite platforms, providing global climatological data sets. Prior instrument comparison studies have shown that there are systematic differences between the droplet size retrieval products (effective radius) of bispectral (e.g., MODIS, Moderate Resolution Imaging Spectroradiometer) and polarimetric (e.g., POLDER, Polarization and Directionality of Earth's Reflectances) instruments. However, intercomparisons of airborne bispectral and polarimetric instruments have yielded results that do not appear to be systematically biased relative to one another. Diagnosing this discrepancy is complicated, because it is often difficult for instrument intercomparison studies to isolate differences between retrieval technique sensitivities and specific instrumental differences such as calibration and atmospheric correction. In addition to these technical differences the polarimetric retrieval is also sensitive to the dispersion of the droplet size distribution (effective variance), which could influence the interpretation of the droplet size retrieval. To avoid these instrument-dependent complications, this study makes use of a cloud remote-sensing retrieval simulator. Created by coupling a large-eddy simulation (LES) cloud model with a 1-D radiative transfer model, the simulator serves as a test bed for understanding differences between bispectral and polarimetric retrievals. With the help of this simulator we can not only compare the two techniques to one another (retrieval intercomparison) but also validate retrievals directly against the LES cloud properties. Using the satellite retrieval simulator, we are able to verify that at high spatial resolution (50 m) the bispectral and polarimetric retrievals are highly correlated with one another within expected observational uncertainties. The relatively small systematic biases at high spatial resolution can be attributed to different sensitivity limitations of the two retrievals. In contrast, a systematic difference between the two retrievals emerges at coarser resolution. This bias largely stems from differences related to sensitivity of the two retrievals to unresolved inhomogeneities in effective variance and optical thickness. The influence of coarse angular resolution is found to increase uncertainty in the polarimetric retrieval but generally maintains a constant mean value.

Miller, Daniel J.↗

NASA NTRS2016

Assessing the Transferability of Statistical Predictive Models for Leaf Area Index Between Two Airborne Discrete Return LiDAR Sensor Designs Within Multiple Intensely Managed Loblolly Pine Forest Locations in the South-Eastern USA

Leaf area is an important forest structural variable which serves as the primary means of mass and energy exchange within vegetated ecosystems. The objective of the current study was to determine if leaf area index (LAI) could be estimated accurately and consistently in five intensively managed pine plantation forests using two multiple-return airborne LiDAR datasets. Field measurements of LAI were made using the LiCOR LAI2000 and LAI2200 instruments within 116 plots were established of varying size and within a variety of stand conditions (i.e. stand age, nutrient regime and stem density) in North Carolina and Virginia in 2008 and 2013. A number of common LiDAR return height and intensity distribution metrics were calculated (e.g. average return height), in addition to ten indices, with two additional variants, utilized in the surrounding literature which have been used to estimate LAI and fractional cover, were calculated from return heights and intensity, for each plot extent. Each of the indices was assessed for correlation with each other, and was used as independent variables in linear regression analysis with field LAI as the dependent variable. All LiDAR derived metrics were also entered into a forward stepwise linear regression. The results from each of the indices varied from an R2 of 0.33 (S.E. 0.87) to 0.89 (S.E. 0.36). Those indices calculated using ratios of all returns produced the strongest correlations, such as the Above and Below Ratio Index (ABRI) and Laser Penetration Index 1 (LPI1). The regression model produced from a combination of three metrics did not improve correlations greatly (R2 0.90; S.E. 0.35). The results indicate that LAI can be predicted over a range of intensively managed pine plantation forest environments accurately when using different LiDAR sensor designs. Those indices which incorporated counts of specific return numbers (e.g. first returns) or return intensity correlated poorly with field measurements. There were disparities between the number of different types of returns and intensity values when comparing the results from two LiDAR sensors, indicating that predictive models developed using such metrics are not transferable between datasets with different acquisition parameters. Each of the indices were significantly correlated with one another, with one exception (LAI proxy), in particular those indices calculated from all returns, which indicates similarities in information content for those indices. It can then be argued that LiDAR indices have reached a similar stage in development to those calculated from optical-spectral sensors, but which offer a number of advantages, such as the reduction or removal of saturation issues in areas of high biomass.

Sumnall, Matthew↗

DOE OSTI2024

Stochastic journeys of cell progenies through compartments and the role of self-renewal, symmetric and asymmetric division

Abstract Division and differentiation events by which cell populations with specific functions are generated often take place as part of a developmental programme, which can be represented by a sequence of compartments. A compartment is the set of cells with common characteristics; sharing, for instance, a spatial location or a phenotype. Differentiation events are transitions from one compartment to the next. Cells may also die or divide. We consider three different types of division events: (i) where both daughter cells inherit the mother’s phenotype (self-renewal), (ii) where only one of the daughters changes phenotype (asymmetric division), and (iii) where both daughters change phenotype (symmetric division). The self-renewal probability in each compartment determines whether the progeny of a single cell, moving through the sequence of compartments, is finite or grows without bound. We analyse the progeny stochastic dynamics with probability generating functions. In the case of self-renewal, by following one of the daughters after any division event, we may construct lifelines containing only one cell at any time. We analyse the number of divisions along such lines, and the compartment where lines terminate with a death event. Analysis and numerical simulations are applied to a five-compartment model of the gradual differentiation of hematopoietic stem cells and to a model of thymocyte development: from pre-double positive to single positive (SP) cells with a bifurcation to either SP4 or SP8 in the last compartment of the sequence.

Science & Technology - Other Topics↗

NASA NTRS1983

Cataractogenesis from high-LET radiation and the Casarett model

The long-term effects on specific animal tissues of exposure to heavy ion irradiation are studied in experiments on cataractogenesis in mice and rabbits. Five groups of rabbits at ages from 8 weeks to 5.3 years were irradiated to a dose of 9.0 Gy of Bragg plateau Ne-20 ions, while mice were exposed to single and total fractionated doses of 4.17 Gy gamma rays or 0.40 to 1.20 Gy of C-12, Ne-20 or Ar-40 particles. Measurements of lens opacities over time show that in the year since irradiation, cataractogenesis is delayed and less severe in rabbits irradiated at age 6 months compared to younger animals, although it is possible that the late effects in adults will surpass those found in the young. The fractionation of the total dose of gamma rays in mice is observed to lead to a marked reduction in the extent of lens opacity after one year, while fractionated heavy ion doses caused a greater degree of posterior lens opacification. Evaluations of the effects of the LET of different ions on cataractogenesis are consistent with RBEs of about 5, 3 and 1-2 for Ar-40, Ne-20 and C-12 ions, respectively. Results thus support the Casarett model of radiation damaging stem cell populations that are not necessarily part of the vasculature.

Cox, A. B.↗

NASA NTRS2017

A Comparison of the SOCIT and DebriSat Experiments

This paper explores the differences between, and shares the lessons learned from, two hypervelocity impact experiments critical to the update of orbital debris environment models. The procedures and processes of the fourth Satellite Orbital Debris Characterization Impact Test (SOCIT) were analyzed and related to the ongoing DebriSat experiment. SOCIT was the first hypervelocity impact test designed specifically for satellites in Low Earth Orbit (LEO). It targeted a 1960's U.S. Navy satellite, from which data was obtained to update pre-existing NASA and DOD breakup models. DebriSat is a comprehensive update to these satellite breakup models- necessary since the material composition and design of satellites have evolved from the time of SOCIT. Specifically, DebriSat utilized carbon fiber, a composite not commonly used in satellites during the construction of the US Navy Transit satellite used in SOCIT. Although DebriSat is an ongoing activity, multiple points of difference are drawn between the two projects. Significantly, the hypervelocity tests were conducted with two distinct satellite models and test configurations, including projectile and chamber layout. While both hypervelocity tests utilized soft catch systems to minimize fragment damage to its post-impact shape, SOCIT only covered 65% of the projected area surrounding the satellite, whereas, DebriSat was completely surrounded cross-range and downrange by the foam panels to more completely collect fragments. Furthermore, utilizing lessons learned from SOCIT, DebriSat's post-impact processing varies in methodology (i.e., fragment collection, measurement, and characterization). For example, fragment sizes were manually determined during the SOCIT experiment, while DebriSat utilizes automated imaging systems for measuring fragments, maximizing repeatability while minimizing the potential for human error. In addition to exploring these variations in methodologies and processes, this paper also presents the challenges DebriSat has encountered thus far and how they were addressed. Accomplishing DebriSat's goal of collecting 90% of the debris, which constitutes well over 100,000 fragments, required addressing many challenges stemming from the very large number of fragments. One of these challenges arose in identifying the foam-embedded fragments. DebriSat addressed this by X-raying all of the panels once the loose debris were removed, and applying a detection algorithm developed in-house to automate the embedded fragment identification process. It is easy to see how the amount of data being compiled would be outstanding. Creating an efficient way to catalog each fragment, as well as archiving the data for reproducibility also posed a great challenge for DebriSat. Barcodes to label each fragment were introduced with the foresight that once the characterization process began, the datasheet for each fragment would have to be accessed again quickly and efficiently. The DebriSat experiment has benefited significantly by leveraging lessons learned from the SOCIT experiment along with the technological advancements that have occurred during the time between the experiments. The two experiments represent two ages of satellite technology and, together, demonstrate the continuous efforts to improve the experimental techniques for fragmentation debris characterization.

Ausay, Erick↗

DOE OSTI2021

Tapping into the physiological responses to mistletoe infection during heat and drought stress

Mistletoes are important co-contributors to tree mortality globally, particularly during droughts. In Australia, mistletoe distributions are expanding in temperate woodlands, while their hosts have experienced unprecedented heat and drought stress in recent years. We investigated whether the excessive water use of mistletoes increased the probability of xylem emboli in a mature woodland during the recent record drought that was compounded by multiple heatwaves. Additionally, we continuously recorded transpiration ($T_{SLA}$) of infected and uninfected branches from two eucalypt species over two summers, monitored stem and leaf water potentials ($\Psi $) and used hydraulic vulnerability curves to estimate percent loss in conductivity (PLC) for each species. Variations in weather (vapor pressure deficit, photosynthetically active radiation, soil water content), host species and % mistletoe foliage explained 78% of hourly $T_{SLA}$. While mistletoe acted as an uncontrollable sink for water in the host even during typical summer days, daily $T_{SLA}$ increased up to 4-fold in infected branches on hot days, highlighting the previously overlooked importance of temperature stress in amplifying water loss in mistletoes. The increased water use of mistletoes resulted in significantly decreased host $\Psi _{\rm{leaf}}$ and $\Psi _{\rm{trunk}}$. It further translated to an estimated increase of up to 11% PLC for infected hosts, confirming greater hydraulic dysfunction of infected trees that place them at higher risk of hydraulic failure. However, uninfected branches of Eucalyptus fibrosa F.Muell. had much tighter controls on water loss than uninfected branches of Eucalyptus moluccana Roxb., which shifted the risk of hydraulic failure towards an increased risk of carbon starvation for E. fibrosa. The contrasting mechanistic responses to heat and drought stress between both co-occurring species demonstrates the complexity of host–parasite interactions and highlights the challenge in predicting species-specific responses to biotic agents in a warmer and drier climate.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2021

Performance Evaluation of Comparative Vacuum Monitoring and Piezoelectric Sensors for Structural Health Monitoring of Rotorcraft Components

The costs associated with the increasing maintenance and surveillance needs of aging structures are rising at an unexpected rate. Multi-site fatigue damage, hidden cracks in hard-to-reach locations, disbonded joints, erosion, impact, and corrosion are among the major flaws encountered in today’s extensive fleet of aging aircraft and space vehicles. Aircraft maintenance and repairs represent about a quarter of a commercial fleet’s operating costs. The application of Structural Health Monitoring (SHM) systems using distributed sensor networks can reduce these costs by facilitating rapid and global assessments of structural integrity. The use of in-situ sensors for real-time health monitoring can overcome inspection impediments stemming from accessibility limitations, complex geometries, and the location and depth of hidden damage. Reliable, structural health monitoring systems can automatically process data, assess structural condition, and signal the need for human intervention. The ease of monitoring an entire on-board network of distributed sensors means that structural health assessments can occur more often, allowing operators to be even more vigilant with respect to flaw onset. SHM systems also allow for condition-based maintenance practices to be substituted for the current time-based or cycle-based maintenance approach thus optimizing maintenance labor. The Federal Aviation Administration has conducted a series of SHM validation and certification programs intended to comprehensively support the evolution and adoption of SHM practices into routine aircraft maintenance practices. This report presents one of those programs involving a Sandia Labs-aviation industry effort to move SHM into routine use for aircraft maintenance. The Airworthiness Assurance NDI Validation Center (AANC) at Sandia Labs, in conjunction with Sikorsky, Structural Monitoring Systems Ltd., Anodyne Electronics Manufacturing Corp., Acellent Technologies Inc., and the Federal Aviation Administration (FAA) carried out a trial validation and certification program to evaluate Comparative Vacuum Monitoring (CVM) and Piezoelectric Transducers (PZT) as a structural health monitoring solution to specific rotorcraft applications. Validation tasks were designed to address the SHM equipment, the health monitoring task, the resolution required, the sensor interrogation procedures, the conditions under which the monitoring will occur, the potential inspector population, adoption of CVM and PZT systems into rotorcraft maintenance programs and the document revisions necessary to allow for their routine use as an alternate means of performing periodic structural inspections. This program addressed formal SHM technology validation and certification issues so that the full spectrum of concerns, including design, deployment, performance and certification were appropriately considered. Sandia Labs designed, implemented, and analyzed the results from a focused and statistically relevant experimental effort to quantify the reliability of a CVM system applied to Sikorsky S-92 fuselage frame application and a PZT system applied to an S-92 main gearbox mount beam application. The applications included both local and global damage detection assessments. All factors that affect SHM sensitivity were included in this program: flaw size, shape, orientation and location relative to the sensors, as well as operational and environmental variables. Statistical methods were applied to performance data to derive Probability of Detection (POD) values for SHM sensors in a manner that agrees with current nondestructive inspection (NDI) validation requirements and is acceptable to both the aviation industry and regulatory bodies. The validation work completed in this program demonstrated the ability of both CVM and PZT SHM systems to detect cracks in rotorcraft components. It proved the ability to use final system response parameters to provide a Green Light/Red Light (“GO” – “NO GO”) decision on the presence of damage. In additional to quantifying the performance of each SHM system for the trial applications on the S-92 platform, this study also identified specific methods that can be used to optimize damage detection, guidance on deployment scenarios that can affect performance and considerations that must be made to properly apply CVM and PZT sensors. These results support the main goal of safely integrating SHM sensors into rotorcraft maintenance programs. Additional benefits from deploying rotorcraft Health and Usage Monitoring Systems (HUMS) may be realized when structural assessment data, collected by an SHM system, is also used to detect structural damage to compliment the operational environment monitoring. The use of in-situ sensors for health monitoring of rotorcraft structures can be a viable option for both flaw detection and maintenance planning activities. This formal SHM validation will allow aircraft manufacturers and airlines to confidently make informed decisions about the proper utilization of CVM and PZT technology. It will also streamline future regulatory actions and formal certification measures needed to assure the safe application of SHM solutions.

42 ENGINEERING↗

DOE OSTI2023

Gradient Amine Sorbents for Low Vacuum Swing CO 2 Capture at Ambient Temperature

The growing concerns over CO 2 emissions have led to the development of various methods for CO 2 capture. CO 2 capture by amine-based sorbents has been achieved by temperature (thermal) swing adsorption (TSA) process, pressure swing adsorption (PSA) process, and temperature pressure or vacuuming swing adsorption (TPSA or TVSA) process for CO 2 capture. Amine sorbents for energy-efficient TSA, PSA, VSA, TPSA or TVSA CO 2 capture should possess a capability which allow adsorbed CO 2 to desorb and amine sorbent to be regenerated at low temperatures or low vacuums. Development of such amine sorbents would significantly decrease energy consumption and sorbent degradation during the regeneration step. The objective of this project is to develop a low vacuum swing adsorption (VSA) process for the capture of CO 2 from air. The focus is on the development of amine sorbent which allows CO 2 to adsorb in the form of weakly adsorbed CO 2 . The weakly adsorbed CO 2 species can be collected from the sorbent by applying a low vacuum at ambient temperature. Specifically, no heating (i.e., thermal energy) is needed for regeneration of amine sorbents. This novel sorbent allows VSA to be operated at ambient temperature without a significant energy demand. This process eliminates the energy-intensive heating and cooling process in temperature swing adsorption (TSA) process. Ambient temperature operation could prolong the lifetime of sorbent and minimize the maintenance cost. Extensive sorbent studies with in-situ infrared spectroscopy have revealed the modifications of conventional amine sorbents with additives can increase the fraction of weakly adsorbed CO 2 . The amine sorbents with loaded CO 2 can be regenerated in part by the following approaches: (i) mild heating to temperatures below 70 °C, (ii) flowing purging gas over modified amine sorbents at temperatures below 40 °C, (iii) vacuuming at pressure below 0.2 atm and temperatures below 40 °C. The performance of these modified amine sorbents has been tested in a 60 grams VSA unit at ambient temperatures. The results show that weakly adsorbed CO 2 which can be desorbed from modified amine sorbents with purging gas at 100 cc/min can also be evacuated at a vacuum of 1 psia at room temperature. A preliminary techno- economic analysis indicates the major cost of the VSA process with modified amine sorbents stems from the operation of vacuum pumps. Thus, developing an excellent sealing with minimum leakage for the VSA unit is the most critical task for further the development of this VSA technology.

36 MATERIALS SCIENCE↗

DOE OSTI2024

Enhancing Nuclear and Radiological Security in Polarized Times: Safeguarding Against Extremist Insider Threats

Domestic violent extremism has been on the rise in recent years, fueled by growing political polarization, the COVID-19 pandemic, and the spread of misinformation online. This concerning trend raises an important question: has this increase in extremist violence manifested in more incidents involving radioactive or nuclear (RN) materials and a corresponding security concern? To explore this question, we analyzed existing data on events involving chemical, biological, radiological, or nuclear materials over time. Specifically, we examined the rate of RN-related incidents, the targets of these events, and the ideologies motivating the perpetrators by using the Violent Non-State Actor Chemical, Biological, Radiological, and Nuclear Event Database from the National Consortium for the Study of Terrorism and Responses to Terrorism. Our analysis aimed to determine whether the broader rise of violent extremism has translated to an elevated risk of attacks involving RN materials. Results of the analysis revealed that there was no clear increase in events involving RN material in the dataset. However, given the rarity of these events, this conclusion does not indicate that such a trend might not appear in coming years if violent extremism continues to rise. To address the potential risk presented by violent extremism, in addition to quantifying the frequency of RN events, we also reviewed traits that could make organizations more susceptible to insider threats from radicalized employees. Focusing on the literature around counterproductive workplace behavior, we identified organizational characteristics that might increase the risk of malicious acts committed by radicalized insiders. These risk factors include abusive leadership, situational constraints, and organizational injustice. Finally, using these organizational risk characteristics, we discuss interventions that organizations with RN material can take to reduce their risk of attacks stemming from violent extremism while still protecting individual rights to privacy and civil liberties. Overall, this article provides an overview of the intersections between rising domestic extremism, insider threats, and risks posed by RN materials. We present current data on trends and patterns in this space as well as practical guidance for organizational resilience.

98 NUCLEAR DISARMAMENT, SAFEGUARDS, AND PHYSICAL P↗

DOE OSTI2020

LHC signals for KK graviton from an extended warped extra dimension

We analyze signals at the Large Hadron Collider (LHC) from production and decay of Kaluza-Klein (KK) gravitons in the context of “extended” warped extra-dimensional models, where the standard model (SM) Higgs and fermion fields are restricted to be in-between the usual ultraviolet/Planck brane and a ~ O(10) TeV (new, “intermediate”) brane, whereas the SM gauge fields (and gravity) propagate further down to the ~ O(TeV) infrared brane. Such a framework suppresses flavor violation stemming from KK particle effects, while keeping the KK gauge bosons and gravitons accessible to the LHC. We find that the signals from KK graviton are significantly different than in the standard warped model. This is because the usually dominant decay modes of KK gravitons into top quark, Higgs and longitudinal W/Z particles are suppressed by the above spatial separation between these two sets of particles, thus other decay channels are allowed to shine themselves. In particular, we focus on two novel decay channels of the KK graviton. The first one is the decay into a pair of radions, each of which decays (dominantly) into a pair of SM gluons, resulting in a resonant 4-jet final state consisting of two pairs of dijet resonance. On the other hand, if the radion is heavier and/or KK gluon is lighter, then the KK graviton mostly decays into a KK gluon and a SM gluon. The resulting KK gluon has a significant decay branching fraction into radion and SM gluon, thereby generating (again) a 4-jet signature, but with a different underlying event topology, i.e., featuring now three different resonances. We demonstrate that the High-Luminosity LHC (HL-LHC) has sensitivity to KK graviton of (up to) ~ 4 TeV in both channels, in the specific model with only gluon field (and gravity) propagating in the extended bulk, whereas it is unlikely to have sensitivity in the standard dijet resonance search channel from KK graviton decay into two gluons.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗