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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 523 records · Page 29

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NASA NTRS2003

Multiple Epstein-Barr virus infections in healthy individuals

We employed a newly developed genotyping technique with direct representational detection of LMP-1 gene sequences to study the molecular epidemiology of Epstein-Barr virus (EBV) infection in healthy individuals. Infections with up to five different EBV genotypes were found in two of nine individuals studied. These results support the hypothesis that multiple EBV infections of healthy individuals are common. The implications for the development of an EBV vaccine are discussed.

Evaluation Studies↗

NASA NTRS1973

A sequence solution to the Fokker-Planck equation.

In this paper a new approach to nonlinear system analysis based on the Fokker-Planck equation is developed. The development begins as a modification of the parametrix method of partial differential equation theory and provides a sequence solution to the multidimensional time-varying Fokker-Planck equation. This sequence is simplified for the case where only a steady-state solution or a solution for the variance of a particular combination of states is desired. Equations are presented in a form suitable for obtaining numerical results using a digital computer.

Mayfield, W. W.↗

NASA NTRS1988

A design and implementation methodology for diagnostic systems

A methodology for design and implementation of diagnostic systems is presented. Also discussed are the advantages of embedding a diagnostic system in a host system environment. The methodology utilizes an architecture for diagnostic system development that is hierarchical and makes use of object-oriented representation techniques. Additionally, qualitative models are used to describe the host system components and their behavior. The methodology architecture includes a diagnostic engine that utilizes a combination of heuristic knowledge to control the sequence of diagnostic reasoning. The methodology provides an integrated approach to development of diagnostic system requirements that is more rigorous than standard systems engineering techniques. The advantages of using this methodology during various life cycle phases of the host systems (e.g., National Aerospace Plane (NASP)) include: the capability to analyze diagnostic instrumentation requirements during the host system design phase, a ready software architecture for implementation of diagnostics in the host system, and the opportunity to analyze instrumentation for failure coverage in safety critical host system operations.

Williams, Linda J. F.↗

NASA NTRS2001

Automatic Debugging Support for UML Designs

Design of large software systems requires rigorous application of software engineering methods covering all phases of the software process. Debugging during the early design phases is extremely important, because late bug-fixes are expensive. In this paper, we describe an approach which facilitates debugging of UML requirements and designs. The Unified Modeling Language (UML) is a set of notations for object-orient design of a software system. We have developed an algorithm which translates requirement specifications in the form of annotated sequence diagrams into structured statecharts. This algorithm detects conflicts between sequence diagrams and inconsistencies in the domain knowledge. After synthesizing statecharts from sequence diagrams, these statecharts usually are subject to manual modification and refinement. By using the "backward" direction of our synthesis algorithm. we are able to map modifications made to the statechart back into the requirements (sequence diagrams) and check for conflicts there. Fed back to the user conflicts detected by our algorithm are the basis for deductive-based debugging of requirements and domain theory in very early development stages. Our approach allows to generate explanations oil why there is a conflict and which parts of the specifications are affected.

Schumann, Johann↗

NASA NTRS1997

A low molecular weight artificial RNA of unique size with multiple probe target regions

Artificial RNAs (aRNAs) containing novel sequence segments embedded in a deletion mutant of Vibrio proteolyticus 5S rRNA have previously been shown to be expressed from a plasmid borne growth rate regulated promoter in E. coli. These aRNAs accumulate to high levels and their detection is a promising tool for studies in molecular microbial ecology and in environmental monitoring. Herein a new construct is described which illustrates the versatility of detection that is possible with aRNAs. This 3xPen aRNA construct carries a 72 nucleotide insert with three copies of a unique 17 base probe target sequence. This aRNA is 160 nucleotides in length and again accumulates to high levels in the E. coli cytoplasm without incorporating into ribosomes. The 3xPen aRNA illustrates two improvements in detection. First, by appropriate selection of insert size, we obtained an aRNA which provides a unique and hence, easily quantifiable peak, on a high resolution gel profile of low molecular weight RNAs. Second, the existence of multiple probe targets results in a nearly commensurate increase in signal when detection is by hybridization. These aRNAs are naturally amplified and carry sequence segments that are not found in known rRNA sequences. It thus may be possible to detect them directly. An experimental step involving RT-PCR or PCR amplification of the gene could therefore be avoided.

Non-NASA Center↗

DOE OSTI2025

Per-Phase Control for CHB Converters with Negative-Sequence Current and DC Voltage Balancing Control

This paper presents a per-phase controller for a grid-connected Cascaded H-Bridge (CHB) converter featuring negative sequence current regulation and DC bus voltage balancing capabilities. Unlike conventional three-phase controllers, each phase of the CHB can be separately and controlled to regulate the DC voltage and reactive power flow. per-phase negative sequence current control strategy is incorporated into the proposed controller to ensure compliance with IEEE Standard 2800-2022, which requires the absorption of negative-sequence reactive current during grid voltage disturbances. Furthermore, a feed-forward control-based DC bus voltage balancing method is employed, which eliminates the need for the integrator commonly used in traditional approaches. The performance of the proposed controller is validated under various unbalanced grid scenarios and DC bus voltage imbalances through electromagnetic transient (EMT) simulation using a three-phase, three-level grid-connected CHB–Dual Active Bridge (DAB) converter testbed developed on MATLAB/Simulink. Additionally, the performance of the negative-sequence current regulation is investigated through simulation under different unbalanced grid voltage scenarios.

negative sequence current↗

NASA NTRS2022

The Psyche Planning Software Subsystem: Creating a Robust Toolset for a Discovery-class Mission

Psyche is a Discovery-class mission to the small metal-rich asteroid (16) Psyche, and is slated to launch in 2022. Psyche, like many missions, requires low-cost activity planning and sequence generation that serves as the backbone to overall uplink design. Such tools must be maintainable over long periods of operations, and powerful enough to solve complex issues that deep-space one-off missions encounter. In this paper we introduce cost-effective solutions that leverage inner- and open-source principles to meet a variety of common and novel use cases.The uplink process that was designed to meet these challenges is presented, as well as the data-flow through the high-level architecture of the planning software subsystem. The user-facing planning tools are described, particularly the Science Opportunity Analyzer, the Plan Editor, Psyche’s planning automation in the Blackbird framework, and Psyche Simulation Reports. All these applications are either new or have been substantially revamped to meet Psyche’s concept of operations. In particular, ensuring the entire toolchain can correctly process epoch-relative activities is discussed. Underlying the main applications are a common set of dependencies developed and maintained by a new cross-mission association of planning developers. In this way, Psyche can inherit well-tested functionality which saves effort and ensures its developers can focus on solving domain challenges. Quality control of the applications and libraries is ensured with a code-review and unit-test based novel ‘CM lite’ process. Collaboration with international industry and academia using the open-source modules is already occurring.The planning and scheduling software is designed to maximize operator awareness of the integrated plan at every step of the process and use common interfaces and file formats to easily transfer information. Design choices plus the team’s test-driven development process enables more expansive capabilities compared to the decentralized planning and sequence generation functions typical of Discovery-class orbiters without significant development cost increases. Benefits and drawbacks of Psyche’s approach are discussed, including comparison to other missions and tools where appropriate.

Ramanathan, Keshav↗

NASA NTRS2011

The Analysis of Rush Orders Risk in Supply Chain: A Simulation Approach

Satisfying customers by delivering demands at agreed time, with competitive prices, and in satisfactory quality level are crucial requirements for supply chain survival. Incidence of risks in supply chain often causes sudden disruptions in the processes and consequently leads to customers losing their trust in a company's competence. Rush orders are considered to be one of the main types of supply chain risks due to their negative impact on the overall performance, Using integrated definition modeling approaches (i.e. IDEF0 & IDEF3) and simulation modeling technique, a comprehensive integrated model has been developed to assess rush order risks and examine two risk mitigation strategies. Detailed functions sequence and objects flow were conceptually modeled to reflect on macro and micro levels of the studied supply chain. Discrete event simulation models were then developed to assess and investigate the mitigation strategies of rush order risks, the objective of this is to minimize order cycle time and cost.

Mahfouz, Amr↗

NASA NTRS2011

MPS Editor

Previously, it was time-consuming to hand-edit data and then set up simulation runs to find the effect and impact of the input data on a spacecraft. MPS Editor provides the user the capability to create/edit/update models and sequences, and immediately try them out using what appears to the user as one piece of software. MPS Editor provides an integrated sequencing environment for users. It provides them with software that can be utilized during development as well as actual operations. In addition, it provides them with a single, consistent, user friendly interface. MPS Editor uses the Eclipse Rich Client Platform to provide an environment that can be tailored to specific missions. It provides the capability to create and edit, and includes an Activity Dictionary to build the simulation spacecraft models, build and edit sequences of commands, and model the effects of those commands on the spacecraft. MPS Editor is written in Java using the Eclipse Rich Client Platform. It is currently built with four perspectives: the Activity Dictionary Perspective, the Project Adaptation Perspective, the Sequence Building Perspective, and the Sequence Modeling Perspective. Each perspective performs a given task. If a mission doesn't require that task, the unneeded perspective is not added to that project's delivery. In the Activity Dictionary Perspective, the user builds the project-specific activities, observations, calibrations, etc. Typically, this is used during the development phases of the mission, although it can be used later to make changes and updates to the Project Activity Dictionary. In the Adaptation Perspective, the user creates the spacecraft models such as power, data store, etc. Again, this is typically used during development, but will be used to update or add models of the spacecraft. The Sequence Building Perspective allows the user to create a sequence of activities or commands that go to the spacecraft. It provides a simulation of the activities and commands that have been created.

Mathews, William S.↗

DOE OSTI2024

A practical approach to using the Genomic Standards Consortium MIxS reporting standard for comparative genomics and metagenomics

Comparative analysis of (meta)genomes necessitates aggregation, integration, and synthesis of well-annotated data using standards. The Genomic Standards Consortium (GSC) collaborates with the research community to develop and maintain the Minimal Information about any (x) Sequence (MIxS) reporting standard for genomic data. To facilitate use of the GSC’s MIxS reporting standard, we provide a description of the structure and terminology, how to navigate ontologies for required terms in MIxS, and demonstrate practical usage through a soil metagenome example.

standards, metadata, genome, metagenome, schema, v↗

DOE OSTI2026

Establishing Data Analysis Pipeline for Bulk ATAC-Seq Datasets

We developed an analysis pipeline for transposase-accessible chromatin sequencing (ATAC-Seq) data derived from bulk samples, which brings together publicly available R packages in addition to command-line tools designed for analysis of bulk ATAC-Seq data and can be run on any computer running a Linux-like operating system such as Ubuntu or Apple OSX.

97 MATHEMATICS AND COMPUTING↗

DOE OSTI2024

Building a FAIR data ecosystem for incorporating single-cell transcriptomics data into agricultural genome to phenome research

Introduction The agriculture genomics community has numerous data submission standards available, but the standards for describing and storing single-cell (SC, e.g., scRNA- seq) data are comparatively underdeveloped. Methods To bridge this gap, we leveraged recent advancements in human genomics infrastructure, such as the integration of the Human Cell Atlas Data Portal with Terra, a secure, scalable, open-source platform for biomedical researchers to access data, run analysis tools, and collaborate. In parallel, the Single Cell Expression Atlas at EMBL-EBI offers a comprehensive data ingestion portal for high-throughput sequencing datasets, including plants, protists, and animals (including humans). Developing data tools connecting these resources would offer significant advantages to the agricultural genomics community. The FAANG data portal at EMBL-EBI emphasizes delivering rich metadata and highly accurate and reliable annotation of farmed animals but is not computationally linked to either of these resources. Results Herein, we describe a pilot-scale project that determines whether the current FAANG metadata standards for livestock can be used to ingest scRNA-seq datasets into Terra in a manner consistent with HCA Data Portal standards. Importantly, rich scRNA-seq metadata can now be brokered through the FAANG data portal using a semi-automated process, thereby avoiding the need for substantial expert curation. We have further extended the functionality of this tool so that validated and ingested SC files within the HCA Data Portal are transferred to Terra for further analysis. In addition, we verified data ingestion into Terra, hosted on Azure, and demonstrated the use of a workflow to analyze the first ingested porcine scRNA-seq dataset. Additionally, we have also developed prototype tools to visualize the output of scRNA-seq analyses on genome browsers to compare gene expression patterns across tissues and cell populations. This JBrowse tool now features distinct tracks, showcasing PBMC scRNA-seq alongside two bulk RNA-seq experiments. Discussion We intend to further build upon these existing tools to construct a scientist-friendly data resource and analytical ecosystem based on Findable, Accessible, Interoperable, and Reusable (FAIR) SC principles to facilitate SC-level genomic analysis through data ingestion, storage, retrieval, re-use, visualization, and comparative annotation across agricultural species.

Genetics & Heredity↗

NASA NTRS1978

Survey of some recent stellar-evolution calculations

The stellar-evolution program developed by Sweigart (1973) was used to compute main sequence turnoff, red giant sequences, and horizontal branch sequences for many values of helium abundance and heavy-element abundance. The immediate aim has been to produce a self-consistent set of evolutionary sequences for wide ranges in the composition and mass. The long-term aim involves application to cluster HR diagrams and the integrated properties of galaxies. Turnoff sequences, red giant sequences, and horizontal branch sequences are discussed.

Sweigart, A. V.↗

NASA NTRS1982

Modeling stiffness loss in boron/aluminum below the fatigue limit

Boron/aluminum can develop significant internal matrix cracking when fatigued. These matrix cracks can result in a 40 percent secant modulus loss in some laminates, even when fatigued below the fatigue limit. It is shown that the same amount of fatigue damage will develop during stress or strain-controlled tests. Stacking sequence has little influence on secant modulus loss. The secant modulus loss in unidirectional composites is small, whereas the losses are substantial in laminates containing off-axis plies. A simple analysis is presented that predicts unnotched laminate secant modulus loss due to fatigue. The analysis is based upon the elastic modulus and Poisson's ratio of the fiber and matrix, fiber volume fraction, fiber orientations, and the cyclic-hardened yield stress of the matrix material. Excellent agreement was achieved between model predictions and experimental results. With this model, designers can project the material stiffness loss for design load or strain levels and assess the feasibility of its use in stiffness-critical parts.

Johnson, W. S.↗

NASA NTRS1984

Method to estimate drag coefficient at the air/ice interface over drifting open pack ice from remotely sensed data

A knowledge in near real time, of the surface drag coefficient for drifting pack ice is vital for predicting its motions. And since this is not routinely available from measurements it must be replaced by estimates. Hence, a method for estimating this variable, as well as the drag coefficient at the water/ice interface and the ice thickness, for drifting open pack ice was developed. These estimates were derived from three-day sequences of LANDSAT-1 MSS images and surface weather charts and from the observed minima and maxima of these variables. The method was tested with four data sets in the southeastern Beaufort sea. Acceptable results were obtained for three data sets. Routine application of the method depends on the availability of data from an all-weather air or spaceborne remote sensing system, producing images with high geometric fidelity and high resolution.

Feldman, U.↗

NASA NTRS1983

Modeling stiffness loss in boron/aluminum laminates below the fatigue limit

Johnson, W. S.↗

NASA NTRS1990

Passive navigation using image irradiance tracking

Rotorcraft operating at low altitudes require navigational schemes for detecting terrain and obstacles. Due to the nature of the missions to be accomplished and available power onboard, a passive navigation scheme is desirable in this situation. The development of a passive navigation scheme using optical image sequences and vehicle motion variables from an onboard inertial navigation scheme is described. This approach combines the geometric properties of perspective projection and a feedback irradiance tracking scheme at each pixel in the image to determine the range to various objects within the field-of-view. Derivation of the numerical algorithm and simulation results are given. Due to the feedback nature of the implementation, the computational scheme is robust. Other applications of the proposed approach include navigation for autonomous planetary rovers and telerobots.

Menon, P. K. A.↗

NASA NTRS1992

Accelerometer method and apparatus for integral display and control functions

Vibration analysis has been used for years to provide a determination of the proper functioning of different types of machinery, including rotating machinery and rocket engines. A determination of a malfunction, if detected at a relatively early stage in its development, will allow changes in operating mode or a sequenced shutdown of the machinery prior to a total failure. Such preventative measures result in less extensive and/or less expensive repairs, and can also prevent a sometimes catastrophic failure of equipment. Standard vibration analyzers are generally rather complex, expensive, and of limited portability. They also usually result in displays and controls being located remotely from the machinery being monitored. Consequently, a need exists for improvements in accelerometer electronic display and control functions which are more suitable for operation directly on machines and which are not so expensive and complex. The invention includes methods and apparatus for detecting mechanical vibrations and outputting a signal in response thereto. The apparatus includes an accelerometer package having integral display and control functions. The accelerometer package is suitable for mounting upon the machinery to be monitored. Display circuitry provides signals to a bar graph display which may be used to monitor machine condition over a period of time. Control switches may be set which correspond to elements in the bar graph to provide an alert if vibration signals increase over the selected trip point. The circuitry is shock mounted within the accelerometer housing. The method provides for outputting a broadband analog accelerometer signal, integrating this signal to produce a velocity signal, integrating and calibrating the velocity signal before application to a display driver, and selecting a trip point at which a digitally compatible output signal is generated. The benefits of a vibration recording and monitoring system with controls and displays readily mountable on the machinery being monitored and having capabilities described will be appreciated by those working in the art.

Bozeman, Richard J., Jr.↗