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Options for Staging Orbits in Cis-Lunar Space

NASA has been studying options to conduct missions beyond Low Earth Orbit, but within the Earth-Moon system, in preparation for deep space exploration including human missions to Mars. Referred to as the Proving Ground, this arena of exploration activities will enable the development of human spaceflight systems and operations to satisfy future exploration objectives beyond the cis-lunar environment. One option being considered includes the deployment of a habitable element or elements, which could be used as a central location for aggregation of supplies and resources for human missions in cis-lunar space and beyond. Characterizing candidate orbit locations for this asset and the impacts on system design and mission operations is important in the overall assessment of the options being considered. The orbits described in this paper were initially selected by taking advantage of previous studies conducted by NASA and the work of other authors. In this paper orbits are assessed for their relative attractiveness based on various factors. First, a set of constraints related to the capability of the combined Orion and SLS system to deliver humans and cargo to and from the orbit are evaluated. Second, the ability to support potential lunar surface activities is considered. Finally, deployed assets intended to spend multiple years in the Proving Ground would ideally require minimal station keeping costs to reduce the mass budget allocated to this function. Additional mission design drivers include potential for uninterrupted communication with deployed assets, thermal, communications, and other operational implications. The results of the characterization and evaluation of the selected orbits indicate a Near Rectilinear Orbit (NRO) is an attractive candidate as an aggregation point or staging location for operations. In this paper, the NRO is further described in terms which balance a number of key attributes that favor a variety of mission classes to meet multiple, sometimes competing, constraints.

Whitley, Ryan

Chain Dynamics in Magnetorheological Suspensions

Magnetorheological (MR) suspensions are composed of colloidal particles which acquire dipole moments when subjected to an external magnetic field. At sufficient field strengths and concentrations, the dipolar particles rapidly aggregate to form long chains. Subsequent lateral cross-linking of the dipolar chains is responsible for a rapid liquid-to-solid-like rheological transition. The unique, magnetically-activated rheological properties of MR suspensions make them ideal for interfacing mechanical systems to electronic controls. Additionally, the ability to experimentally probe colloidal suspensions interacting through tunable anisotropic potentials is of fundamental interest. Our current experimental work has focused on understanding the fluctuations of dipolar chains. It has been proposed by Halsey and Toor (HT) that the strong Landau-Peierls thermal fluctuations of dipolar chains could be responsible for long-range attractions between chains. Such interactions will govern the long-time relaxation of MR suspensions. We have synthesized monodisperse neutrally buoyant MR suspensions by density matching stabilized ferrofluid emulsion droplets with D2O. This allows us to probe the dynamics of the dipolar chains using light scattering without gravitational, interfacial, and polydispersity effects to resolve the short-wavelength dynamics of the dipolar chains. We used diffusing wave spectroscopy to measure these dynamics. The particle displacements at short times that show an independence to the field strength, but at long times exhibit a constrained, sub-diffusive motion that slows as the dipole strength is increased. The experiments are in good qualitative agreement with Brownian dynamics simulations of dipolar chains. Although there have been several important and detailed studies of the structure and interactions in MR suspensions, there has not been conclusive evidence that supports or contradicts the HT model prediction that long-range interactions exist between fluctuating chains of dipolar particles. Resolving this issue would contribute greatly to the understanding of these interesting and important materials. We have begun to test the predictions of the HT model by both examining the dynamics of individual chains and by measuring the forces between dipolar chains directly to accurately and quantitatively assess the interactions that they experience. To do so, we employ optical trapping techniques and video-microscopy to manipulate and observe our samples on the microscopic level. With these techniques, it is possible to observe chains that are fluctuating freely in three-dimensions, independent of interfacial effects. More importantly, we are able to controllably observe the interactions of two chains at various separations to measure the force-distance profile. The techniques also allow us to study the mechanical properties of individual chains and chain clusters. Our work to this point has focused on reversibly-formed dipolar chains due to field induced dipoles where the combination of this chaining, the dipolar forces, and the hydrodynamic interactions that dictate the rheology of the suspensions. One can envision, however, many situations where optical, electronic, or rheological behavior may be optimized with magneto-responsive anisotropic particles. Chains of polarizable particles may have the best properties as they can coil and flex in the absence of a field and stiffen and orient when a field is applied. We have recently demonstrated a synthesis of stable, permanent paramagnetic chains by both covalently and physically linking paramagnetic colloidal particles. The method employed allows us to create monodisperse chains of controlled length. We observed the stability, field-alignment, and rigidity of this new class of materials. The chains may exhibit unique rheological properties in an applied magnetic field over isotropic suspensions of paramagnetic particles. They are also useful rheological models as bead-spring systems. These chains form the basis for our current experiments with optical traps.

Gast, A. P.

Building access and community standards for opacity data at the onset of next-generation atmosphere observations

The characterization of a diverse set of exoplanet atmosphere observations, ranging from hot gas giants to small temperate rocky worlds, will be one of the legacies of upcoming facilities such as the James Webb Space Telescope (JWST). Our understanding and interpretation of such observations will hinge on our ability to link observations with atmospheric theoretical studies that critically rely on fundamental molecular and atomic opacities. Computing such opacities is a highly non-trivial and inaccessible process which requires several terabytes of available disk space, hours of CPU time per pressure-temperature combination, and requires users to carefully aggregate line lists data from various sources, which limits access and intercomparison of opacity data in the exoplanet community. Here we present MAESTRO (Molecules and Atoms in Exoplanet Science: Tools and Resources for Opacities) an opacity database that can be accessed by the community via a web interface and python API. MAESTRO was built with community input to create a version-controlled opacity database that is easily queryable, includes informative metadata to ensure reproducibility, and exports relevant citations for inclusion in publications. Scheduled for community release in 2022, MAESTRO will prove to be an invaluable community resource in the era of JWST and beyond.

Natasha Batalha

NAS Demand Predictions, Transportation Systems Analysis Model (TSAM) Compared with Other Forecasts

The current work incorporates the Transportation Systems Analysis Model (TSAM) to predict the future demand for airline travel. TSAM is a multi-mode, national model that predicts the demand for all long distance travel at a county level based upon population and demographics. The model conducts a mode choice analysis to compute the demand for commercial airline travel based upon the traveler s purpose of the trip, value of time, cost and time of the trip,. The county demand for airline travel is then aggregated (or distributed) to the airport level, and the enplanement demand at commercial airports is modeled. With the growth in flight demand, and utilizing current airline flight schedules, the Fratar algorithm is used to develop future flight schedules in the NAS. The projected flights can then be flown through air transportation simulators to quantify the ability of the NAS to meet future demand. A major strength of the TSAM analysis is that scenario planning can be conducted to quantify capacity requirements at individual airports, based upon different future scenarios. Different demographic scenarios can be analyzed to model the demand sensitivity to them. Also, it is fairly well know, but not well modeled at the airport level, that the demand for travel is highly dependent on the cost of travel, or the fare yield of the airline industry. The FAA projects the fare yield (in constant year dollars) to keep decreasing into the future. The magnitude and/or direction of these projections can be suspect in light of the general lack of airline profits and the large rises in airline fuel cost. Also, changes in travel time and convenience have an influence on the demand for air travel, especially for business travel. Future planners cannot easily conduct sensitivity studies of future demand with the FAA TAF data, nor with the Boeing or Airbus projections. In TSAM many factors can be parameterized and various demand sensitivities can be predicted for future travel. These resulting demand scenarios can be incorporated into future flight schedules, therefore providing a quantifiable demand for flights in the NAS for a range of futures. In addition, new future airline business scenarios are investigated that illustrate when direct flights can replace connecting flights and larger aircraft can be substituted, only when justified by demand.

Viken, Jeff

Utilizing Traveler Demand Modeling to Predict Future Commercial Flight Schedules in the NAS

The current work incorporates the Transportation Systems Analysis Model (TSAM) to predict the future demand for airline travel. TSAM is a multi-mode, national model that predicts the demand for all long distance travel at a county level based upon population and demographics. The model conducts a mode choice analysis to compute the demand for commercial airline travel based upon the traveler s purpose of the trip, value of time, cost and time of the trip,. The county demand for airline travel is then aggregated (or distributed) to the airport level, and the enplanement demand at commercial airports is modeled. With the growth in flight demand, and utilizing current airline flight schedules, the Fratar algorithm is used to develop future flight schedules in the NAS. The projected flights can then be flown through air transportation simulators to quantify the ability of the NAS to meet future demand. A major strength of the TSAM analysis is that scenario planning can be conducted to quantify capacity requirements at individual airports, based upon different future scenarios. Different demographic scenarios can be analyzed to model the demand sensitivity to them. Also, it is fairly well know, but not well modeled at the airport level, that the demand for travel is highly dependent on the cost of travel, or the fare yield of the airline industry. The FAA projects the fare yield (in constant year dollars) to keep decreasing into the future. The magnitude and/or direction of these projections can be suspect in light of the general lack of airline profits and the large rises in airline fuel cost. Also, changes in travel time and convenience have an influence on the demand for air travel, especially for business travel. Future planners cannot easily conduct sensitivity studies of future demand with the FAA TAF data, nor with the Boeing or Airbus projections. In TSAM many factors can be parameterized and various demand sensitivities can be predicted for future travel. These resulting demand scenarios can be incorporated into future flight schedules, therefore providing a quantifiable demand for flights in the NAS for a range of futures. In addition, new future airline business scenarios are investigated that illustrate when direct flights can replace connecting flights and larger aircraft can be substituted, only when justified by demand.

Viken, Jeff

Effect of Flow on Gene Regulation in Smooth Muscle Cells and Macromolecular Transport Across Endothelial Cell Monolayers

Endothelial cells line all of the vessels of the circulatory system, providing a non-thrombogenic conduit for blood flow; they regulate many complex functions in the vasculature, such as coagulation, fibrinolysis, platelet aggregation, vessel tone and growth, and leukocyte traffic; and they form the principal barrier to transport of substances between the blood and the surrounding tissue space. The permeability of endothelial cell changes with environmental stimuli; shear stress, in particular, applied either in vivo, or in vitro, induces changes in protein expression and secretion of vasoactive factors by endothelial cells. The ability to study the effects of shear on the macromolecular permeability of the cerebral vasculature is particularly important, since in no other place is the barrier function of the endothelium more important than in the brain. The endothelial cells of this organ have developed special barrier properties that keep the cerebral system from experiencing any drastic change in composition; together with glial cells, they form the blood brain barrier (BBB). We have studied the effect of flow on bovine BBB using flow chambers and tissue culture systems.

McIntire, Larry V.

Nonconservative force model parameter estimation strategy for TOPEX/Poseidon precision orbit determination

The TOPEX/Poseidon spacecraft was launched on August 10, 1992 to study the Earth's oceans. To achieve maximum benefit from the altimetric data it is to collect, mission requirements dictate that TOPEX/Poseidon's orbit must be computed at an unprecedented level of accuracy. To reach our pre-launch radial orbit accuracy goals, the mismodeling of the radiative nonconservative forces of solar radiation, Earth albedo an infrared re-radiation, and spacecraft thermal imbalances cannot produce in combination more than a 6 cm rms error over a 10 day period. Similarly, the 10-day drag modeling error cannot exceed 3 cm rms. In order to satisfy these requirements, a 'box-wing' representation of the satellite has been developed in which, the satellite is modelled as the combination of flat plates arranged in the shape of a box and a connected solar array. The radiative/thermal nonconservative forces acting on each of the eight surfaces are computed independently, yielding vector accelerations which are summed to compute the total aggregate effect on the satellite center-of-mass. Select parameters associated with the flat plates are adjusted to obtain a better representation of the satellite acceleration history. This study analyzes the estimation of these parameters from simulated TOPEX/Poseidon laser data in the presence of both nonconservative and gravity model errors. A 'best choice' of estimated parameters is derived and the ability to meet mission requirements with the 'box-wing' model evaluated.

Luthcke, S. B.

Modeling radiation forces acting on TOPEX/Poseidon for precision orbit determination

Geodetic satellites such as GEOSAT, SPOT, ERS-1, and TOPEX/Poseidon require accurate orbital computations to support the scientific data they collect. Until recently, gravity field mismodeling was the major source of error in precise orbit definition. However, albedo and infrared re-radiation, and spacecraft thermal imbalances produce in combination no more than a 6-cm radial root-mean-square (RMS) error over a 10-day period. This requires the development of nonconservative force models that take the satellite's complex geometry, attitude, and surface properties into account. For TOPEX/Poseidon, a 'box-wing' satellite form was investigated that models the satellite as a combination of flat plates arranged in a box shape with a connected solar array. The nonconservative forces acting on each of the eight surfaces are computed independently, yielding vector accelerations which are summed to compute the total aggregate effect on the satellite center-of-mass. In order to test the validity of this concept, 'micro-models' based on finite element analysis of TOPEX/Poseidon were used to generate acceleration histories in a wide variety of orbit orientations. These profiles are then compared to the box-wing model. The results of these simulations and their implication on the ability to precisely model the TOPEX/Poseidon orbit are discussed.

Marshall, J. A.

Phylogenetic Conservatism in Plant Phenology

Phenological events defined points in the life cycle of a plant or animal have been regarded as highly plastic traits, reflecting flexible responses to various environmental cues. The ability of a species to track, via shifts in phenological events, the abiotic environment through time might dictate its vulnerability to future climate change. Understanding the predictors and drivers of phenological change is therefore critical. Here, we evaluated evidence for phylogenetic conservatism the tendency for closely related species to share similar ecological and biological attributes in phenological traits across flowering plants. We aggregated published and unpublished data on timing of first flower and first leaf, encompassing 4000 species at 23 sites across the Northern Hemisphere. We reconstructed the phylogeny for the set of included species, first, using the software program Phylomatic, and second, from DNA data. We then quantified phylogenetic conservatism in plant phenology within and across sites. We show that more closely related species tend to flower and leaf at similar times. By contrasting mean flowering times within and across sites, however, we illustrate that it is not the time of year that is conserved, but rather the phenological responses to a common set of abiotic cues. Our findings suggest that species cannot be treated as statistically independent when modelling phenological responses.Closely related species tend to resemble each other in the timing of their life-history events, a likely product of evolutionarily conserved responses to environmental cues. The search for the underlying drivers of phenology must therefore account for species' shared evolutionary histories.

phylogenetic conservatism

Community-Wide Validation of Geospace Model Local K-Index Predictions to Support Model Transition to Operations

We present the latest result of a community-wide space weather model validation effort coordinated among the Community Coordinated Modeling Center (CCMC), NOAA Space Weather Prediction Center (SWPC), model developers, and the broader science community. Validation of geospace models is a critical activity for both building confidence in the science results produced by the models and in assessing the suitability of the models for transition to operations. Indeed, a primary motivation of this work is supporting NOAA/SWPCs effort to select a model or models to be transitioned into operations. Our validation efforts focus on the ability of the models to reproduce a regional index of geomagnetic disturbance, the local K-index. Our analysis includes six events representing a range of geomagnetic activity conditions and six geomagnetic observatories representing midlatitude and high-latitude locations. Contingency tables, skill scores, and distribution metrics are used for the quantitative analysis of model performance. We consider model performance on an event-by-event basis, aggregated over events, at specific station locations, and separated into high-latitude and midlatitude domains. A summary of results is presented in this report, and an online tool for detailed analysis is available at the CCMC.

Glocer, A.

The Resolved Stellar Population in 50 Regions of M83 from HST/WFC3 Early Release Science Observations

We present a multi-wavelength photometric study of approximately 15,000 resolved stars in the nearby spiral galaxy M83 (NGC 5236, D = 4.61 Mpc) based on Hubble Space Telescope Wide Field Camera 3 observations using four filters: F336W, F438W, F555W, and F814W. We select 50 regions (an average size of 260 pc by 280 pc) in the spiral arm and inter-arm areas of M83 and determine the age distribution of the luminous stellar populations in each region. This is accomplished by correcting for extinction toward each individual star by comparing its colors with predictions from stellar isochrones.We compare the resulting luminosity-weighted mean ages of the luminous stars in the 50 regions with those determined from several independent methods, including the number ratio of red-to-blue supergiants, morphological appearance of the regions, surface brightness fluctuations, and the ages of clusters in the regions. We find reasonably good agreement between these methods. We also find that young stars are much more likely to be found in concentrated aggregates along spiral arms, while older stars are more dispersed. These results are consistent with the scenario that star formation is associated with the spiral arms, and stars form primarily in star clusters and then disperse on short timescales to form the field population. The locations ofWolf-Rayet stars are found to correlate with the positions of many of the youngest regions, providing additional support for our ability to accurately estimate ages. We address the effects of spatial resolution on the measured colors, magnitudes, and age estimates. While individual stars can occasionally show measurable differences in the colors and magnitudes, the age estimates for entire regions are only slightly affected.

Hubble Space Telescope

Morphology and types of particles of regolith sample from the Sea of Fertility

A brief description of the morphology of lunar surface material returned by Luna 16 automatic lunar station is given. Adhesiveness of the surface material and its ability to be electrified is noted. Two main genetic groups of regolith particles are differentiated: primary, represented mainly by fragments of magmatic rocks of the basalt and gabbro types, as well as mineralized grains of their constituent minerals, and secondary, particles subjected to appreciable exogenic transformation on the surface of the Moon. The second group, representing more than 70 percent of the material in coarse fractions, includes particles of breccias, sintered aggregates of complex dendritic form, and glass and vitrified particles of varied composition.

Florenskiy, K. P.

Space Biotech: Hindsight, Insight, Foresight

Over the past forty years, microgravity has inspired and enabled applications in a wide range of sectors including medicine, materials, computers, communications, and national defense. Trends show that demand for high-tech solutions is increasing in these sectors, solutions that require higher resolution, greater precision, novel materials, innovative processes, and more sophisticated tools. These are areas where microgravity can offer unique capabilities for innovation. The Emerging Space Office (ESO) has engaged in multiple studies over the past year that have found that microgravity RD is one of the most promising technology areas for contributing to economic growth and to NASAs mission. The focus of these studies was on terrestrial markets rather than NASA applications, applied research rather than basic research, and commercial rather than academic investigators. There have been more success stories than are generally appreciated and there are significant areas of promising future potential. Many of the problems that have limited commercial microgravity development in the past are being solved. Microgravity research and development (RD) requires iteration and learning, as rapidly as possible. New technologies enable high throughput and rapid data collection in increasingly small payloads. The International Space Station is in orbit and provides a laboratory that is available 247 at least until 2024. Frequent flights by commercial space providers to and from the ISS now enable the fast learning cycles needed by high-tech industries. Launch costs are decreasing and the ability to return payloads to Earth is increasing. New commercial space laboratories, such as those being developed by SpaceX and Bigelow Aerospace, are in the final stages of development and testing. This ecosystem for microgravity RD has never been available before. These are game-changer conditions for attracting high-tech industries to space for terrestrial, as well as NASA, applications. However, few know that these capabilities are available or how to use them. In aggregate, the potential value for new applications from microgravity RD over the next ten years could add billions of dollars per year in terrestrial applications to the future economy, create new jobs, and generate a wide range of public benefits in medical advances, while broadening the customer base for the emerging space industry.

biotech

Revamping Spacecraft Operational Intelligence with Splunk

So what is Splunk? Instead of giving the technical details, which you can find online, I'll tell you what it did for me. Splunk slapped everything into one place, with one uniform format, and gave me the ability to forget about all these annoying details of where it is, how to parse it, and all that. Instead, I only need to interact with Splunk to find the data I need. This sounds simple and obvious, but it's surprising what you can do once you all of your data is indexed in one place. By having your data organized, querying becomes much easier. Let's say that I want to search telemetry for a sensor_name gtemp_1 h and to return all data that is at most five minutes old. And because Splunk can hook into a real ]time stream, this data will always be up-to-date. Extending the previous example, I can now aggregate all types of data into one view based in time. In this picture, I've got transaction logs, telemetry, and downlinked files all in one page, organized by time. Even though the raw data looks completely than this, I've defined interfaces that transform it into this uniform format. This gives me a more complete picture for the question what was the spacecraft doing at this particular time? And because querying data is simple, I can start with a big block of data and whiddle it down to what I need, rather than hunting around for the individual pieces of data that I need. When we have all the data we need, we can begin widdling down the data with Splunk's Unix-like search syntax. These three examples highlights my trial-and-error attempts to find large temperature changes. I begin by showing the first 5 temperatures, only to find that they're sorted chronologically, rather than from highest temperatures to lowest temperatures. The next line shows sorting temperatures by their values, but I find that that fs not really what I want either. I want to know the delta temperatures between readings. Looking through Splunk's user manual, I find the delta function, which lets me dynamically generate new information to use in my query. With that extra piece of information, I can now return only the telemetry readings where the temperature changed by at least 10. One other useful feature I'll mention is that all of these queries can be run through Splunk's API. So any scripting language you can think of can plug right in and make these queries. This gives us the ability to build a lot of new tools.

operational intelligence

A Methodology for Reliability Assessments of Arbitrary Satellite Constellations

The commercial success of small satellite development and multi-satellite missions has led to constellation missions being proposed in every sector of the market. This paradigm shift has the potential to enable more capable and cost-effective space science missions. However, these novel approaches to space missions have outstripped existing policy and analysis methods for assessing the reliability of multi-satellite missions. Mission designers and decisionmakers need new methods and standards for modeling how the reliability of constituent spacecraft elements affect the aggregate reliability of constellations across a wide range of concepts and application-specific requirements. The Space Mission Analysis Branch at NASA’s Langley Research Center has developed a methodology for simulating the on-orbit reliability of arbitrary spacecraft constellations. In this paper, the authors describe the methodology and provides examples of how it can be used to evaluate specific constellation designs. To demonstrate the flexibility of the approach, several different constellation configurations are considered, including both homogenous and heterogenous designs. Next, the authors demonstrate the ability to perform broad parameter sweeps to develop greater insight into the intrinsic reliability trends associated with constellation concepts. Finally, the authors provide tables and novel data graphics which enable decisionmakers to quickly discern the relationships between constellation sizes, topologies, replenishment strategies, and overall constellation reliability.

satellite

Mammalian development in space

Life on Earth, and thus the reproductive and ontogenetic processes of all extant species and their ancestors, evolved under the constant influence of the Earth's l g gravitational field. These considerations raise important questions about the ability of mammals to reproduce and develop in space. In this chapter, I review the current state of our knowledge of spaceflight effects on developing mammals. Recent studies are revealing the first insights into how the space environment affects critical phases of mammalian reproduction and development, viz., those events surrounding fertilization, embryogenesis, pregnancy, birth, postnatal maturation and parental care. This review emphasizes fetal and early postnatal life, the developmental epochs for which the greatest amounts of mammalian spaceflight data have been amassed. The maternal-offspring system, the coordinated aggregate of mother and young comprising mammalian development, is of primary importance during these early, formative developmental phases. The existing research supports the view that biologically meaningful interactions between mothers and offspring are changed in the weightlessness of space. These changes may, in turn, cloud interpretations of spaceflight effects on developing offspring. Whereas studies of mid-pregnant rats in space have been extraordinarily successful, studies of young rat litters launched at 9 days of postnatal age or earlier, have been encumbered with problems related to the design of in-flight caging and compromised maternal-offspring interactions. Possibilities for mammalian birth in space, an event that has not yet transpired, are considered. In the aggregate, the results indicate a strong need for new studies of mammalian reproduction and development in space. Habitat development and systematic ground-based testing are important prerequisites to future research with young postnatal rodents in space. Together, the findings support the view that the environment within which young mammals develop, comprised of its mother and siblings, is of paramount importance in interpreting spaceflight effects.

Review

Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis

The lack of readily available experimental systems has limited knowledge pertaining to the development of Salmonella-induced gastroenteritis and diarrheal disease in humans. We used a novel low-shear stress cell culture system developed at the National Aeronautics and Space Administration in conjunction with cultivation of three-dimensional (3-D) aggregates of human intestinal tissue to study the infectivity of Salmonella enterica serovar Typhimurium for human intestinal epithelium. Immunohistochemical characterization and microscopic analysis of 3-D aggregates of the human intestinal epithelial cell line Int-407 revealed that the 3-D cells more accurately modeled human in vivo differentiated tissues than did conventional monolayer cultures of the same cells. Results from infectivity studies showed that Salmonella established infection of the 3-D cells in a much different manner than that observed for monolayers. Following the same time course of infection with Salmonella, 3-D Int-407 cells displayed minimal loss of structural integrity compared to that of Int-407 monolayers. Furthermore, Salmonella exhibited significantly lower abilities to adhere to, invade, and induce apoptosis of 3-D Int-407 cells than it did for infected Int-407 monolayers. Analysis of cytokine expression profiles of 3-D Int-407 cells and monolayers following infection with Salmonella revealed significant differences in expression of interleukin 1alpha (IL-1alpha), IL-1beta, IL-6, IL-1Ra, and tumor necrosis factor alpha mRNAs between the two cultures. In addition, uninfected 3-D Int-407 cells constitutively expressed higher levels of transforming growth factor beta1 mRNA and prostaglandin E2 than did uninfected Int-407 monolayers. By more accurately modeling many aspects of human in vivo tissues, the 3-D intestinal cell model generated in this study offers a novel approach for studying microbial infectivity from the perspective of the host-pathogen interaction.

NASA Discipline Environmental Health

Demand modelling of passenger air travel: An analysis and extension, volume 2

Previous intercity travel demand models in terms of their ability to predict air travel in a useful way and the need for disaggregation in the approach to demand modelling are evaluated. The viability of incorporating non-conventional factors (i.e. non-econometric, such as time and cost) in travel demand forecasting models are determined. The investigation of existing models is carried out in order to provide insight into their strong points and shortcomings. The model is characterized as a market segmentation model. This is a consequence of the strengths of disaggregation and its natural evolution to a usable aggregate formulation. The need for this approach both pedagogically and mathematically is discussed. In addition this volume contains two appendices which should prove useful to the non-specialist in the area.

Jacobson, I. D.