Results for “CA activity” — Page 3

NASA NTRS1983

Differences in the temporal variations of solar UV flux, 10.7-cm solar radio flux, sunspot number, and Ca-K plage data caused by solar rotation and active region evolution

Attention is given to two types of temporal variations in the solar UV spectral irradiance caused by solar rotation and active region evolution. It is noted that the first type of dissimilar temporal behavior occurs when concentrations of solar active regions evolve at solar longitudes nearly 180 deg apart. Both the UV observations and modeled UV fluxes based on Ca-K plage data then exhibit pronounced 13-day periodicity, whereas the 10.7-cm solar radio flux and sunspot number exhibit quite dissimilar temporal variations. This type of dissimilarity is related to the modeled UV flux and has a dependence on the solar central meridian distance that is narrower than that for the 10.7-cm radio flux or for sunspot numbers. A second case of marked dissimilarity is seen when major new solar active regions arise and dominate the full-disk fluxes for several rotations. It is found that the strongest peaks in 10.7 cm and sunspot numbers tend to occur on their first rotation, for example, during major dips in the total solar irradiance, whereas the Ca-K plages and UV enhancements peak on the next rotation and then decay more slowly on subsequent rotations.

Donnelly, R. F.↗

NASA NTRS1987

Spacelab Life Sciences 1 and 2 scientific research objectives

The pressurized Spacelab module was designed and built to allow investigators to conduct research in space in an environment approximating that of a ground-based laboratory. It is configured to allow multiple investigations employing both human and nonhuman subjects. This flexability is exemplified by the SLS-1, SLS-2, and SLS-3 experiment complement. A total of 21 experiments are scheduled for these missions; the areas to be investigated are renal/endocrine function, cardiovascular/cardiopulmonary function, hematology, immunology, metabolic activity of muscle, Ca metabolism, the vestibular system, and general biology. A plan for integration of measurements will allow each investigator to use data from other experiments. The experiments make up a scientifically balanced payload that addresses fundamental biomedical problems associated with space flight and provides the first opportunity to study the acute effects of weightlessness in a comprehensive, interrelated fashion.

Leach, Carolyn S.↗

NASA NTRSDate not supplied

A Human-in-the-Loop Evaluation of ACAS Xu

As part of the Phase 2 UAS DAA MOPS, a Class 3 DAA system has been under development with the potential to resolve many of the limitations of Class 1 and 2 systems. A Class 3 system would combine the DAA and CA functions into a single, unified system, and would also extend the CA capabilities relative to TCAS II. Class 3 is enabled by the Airborne Collision Avoidance System (ACAS) XU, a next generation CA system developed specifically for UAS operations. Unlike Class 2 systems, ACAS XU provides CA protection against both cooperative and non-cooperative traffic. Class 3 systems also expand the CA logic to allow horizontal RAs in addition to vertical RAs. The purpose of the current study was to evaluate ACAS XU in a real-time, HITL simulation. The latest version of ACAS XU was implemented and pilots were tasked with responding to scripted traffic conflicts over the course of four experimental trials. Variables included the location of the ACAS XU guidance information (standalone vs. integrated) and traffic conflict type (DAA or CA threat). Sixteen active UAS pilots participated in the study, with ATC and ‘pseudo’ pilots acting as airspace confederates. Results showed that pilots were able to maintain DWC with ACAS XU at a rate comparable to previous research (~5%). Compliance rates to initial RAs were high (~90%) but dropped significantly when the target heading value issued during horizontal RAs were updated over the course of an encounter (30-70%). The implications of these findings on the display, alerting, and guidance requirements for Class 3 systems will be discussed.

unmanned aircraft systems↗

NASA NTRSDate not supplied

A Human-in-the-Loop Evaluation of ACAS Xu

As part of the Phase 2 UAS DAA MOPS, a Class 3 DAA system has been under development with the potential to resolve many of the limitations of Class 1 and 2 systems. A Class 3 system would combine the DAA and CA functions into a single, unified system, and would also extend the CA capabilities relative to TCAS II. Class 3 is enabled by the Airborne Collision Avoidance System (ACAS) XU, a next generation CA system developed specifically for UAS operations. Unlike Class 2 systems, ACAS XU provides CA protection against both cooperative and non-cooperative traffic. Class 3 systems also expand the CA logic to allow horizontal RAs in addition to vertical RAs. The purpose of the current study was to evaluate ACAS XU in a real-time, HITL simulation. The latest version of ACAS XU was implemented and pilots were tasked with responding to scripted traffic conflicts over the course of four experimental trials. Variables included the location of the ACAS XU guidance information (standalone vs. integrated) and traffic conflict type (DAA or CA threat). Sixteen active UAS pilots participated in the study, with ATC and ‘pseudo’ pilots acting as airspace confederates. Results showed that pilots were able to maintain DWC with ACAS XU at a rate comparable to previous research (~5%). Compliance rates to initial RAs were high (~90%) but dropped significantly when the target heading value issued during horizontal RAs were updated over the course of an encounter (30-70%). The implications of these findings on the display, alerting, and guidance requirements for Class 3 systems will be discussed.

unmanned aircraft systems↗

NASA NTRS2020

A Human-in-theLoop Evaluation of ACAS Xu

As part of the Phase 2 UAS DAA MOPS, a Class 3 DAA system has been under development with the potential to resolve many of the limitations of Class 1 and 2 systems. A Class 3 system would combine the DAA and CA functions into a single, unified system, and would also extend the CA capabilities relative to TCAS II. Class 3 is enabled by the Airborne Collision Avoidance System (ACAS) XU, a next generation CA system developed specifically for UAS operations. Unlike Class 2 systems, ACAS XU provides CA protection against both cooperative and non-cooperative traffic. Class 3 systems also expand the CA logic to allow horizontal RAs in addition to vertical RAs. The purpose of the current study was to evaluate ACAS XU in a real-time, HITL simulation. The latest version of ACAS XU was implemented and pilots were tasked with responding to scripted traffic conflicts over the course of four experimental trials. Variables included the location of the ACAS XU guidance information (standalone vs. integrated) and traffic conflict type (DAA or CA threat). Sixteen active UAS pilots participated in the study, with ATC and ‘pseudo’ pilots acting as airspace confederates. Results showed that pilots were able to maintain DWC with ACAS XU at a rate comparable to previous research (~5%). Compliance rates to initial RAs were high (~90%) but dropped significantly when the target heading value issued during horizontal RAs were updated over the course of an encounter (30-70%). The implications of these findings on the display, alerting, and guidance requirements for Class 3 systems will be discussed.

unmanned aircraft systems↗

NASA NTRS2000

Calmodulin activation of an endoplasmic reticulum-located calcium pump involves an interaction with the N-terminal autoinhibitory domain

To investigate how calmodulin regulates a unique subfamily of Ca(2+) pumps found in plants, we examined the kinetic properties of isoform ACA2 identified in Arabidopsis. A recombinant ACA2 was expressed in a yeast K616 mutant deficient in two endogenous Ca(2+) pumps. Orthovanadate-sensitive (45)Ca(2+) transport into vesicles isolated from transformants demonstrated that ACA2 is a Ca(2+) pump. Ca(2+) pumping by the full-length protein (ACA2-1) was 4- to 10-fold lower than that of the N-terminal truncated ACA2-2 (Delta2-80), indicating that the N-terminal domain normally acts to inhibit the pump. An inhibitory sequence (IC(50) = 4 microM) was localized to a region within valine-20 to leucine-44, because a peptide corresponding to this sequence lowered the V(max) and increased the K(m) for Ca(2+) of the constitutively active ACA2-2 to values comparable to the full-length pump. The peptide also blocked the activity (IC(50) = 7 microM) of a Ca(2+) pump (AtECA1) belonging to a second family of Ca(2+) pumps. This inhibitory sequence appears to overlap with a calmodulin-binding site in ACA2, previously mapped between aspartate-19 and arginine-36 (J.F. Harper, B. Hong, I. Hwang, H.Q. Guo, R. Stoddard, J.F. Huang, M.G. Palmgren, H. Sze inverted question mark1998 J Biol Chem 273: 1099-1106). These results support a model in which the pump is kept "unactivated" by an intramolecular interaction between an autoinhibitory sequence located between residues 20 and 44 and a site in the Ca(2+) pump core that is highly conserved between different Ca(2+) pump families. Results further support a model in which activation occurs as a result of Ca(2+)-induced binding of calmodulin to a site overlapping or immediately adjacent to the autoinhibitory sequence.

NASA Program Fundamental Space Biology↗

NASA NTRS1984

Age-rotation relationship for late-type main-sequence stars

With advancing spectral type and increasing age, late main-sequence stars exhibit monotonic decrease in rotational velocity. It is of great interest to extend the rotation-age relationship to stars of later spectral type. In recent times it has become possible to measure directly the rotational periods from the photometric modulation by Ca II H and K line emission. There have also been successful attempts to relate the chromospheric activity as manifested through Ca II H and K lines to the rotation period, and it was shown that the fraction of total stellar luminosity in Ca II H and K lines, corrected for photospheric contribution, is a function of a single parameter related to P and B-V. In the present investigation, this rotation-activity relation is utilized to infer the rotation periods as a function of spectral type. The period versus B-V plot is employed as a basis to infer that the rotational period of main-sequence stars is a single-valued function of mass (B-V color) and age.

Rengarajan, T. N.↗

NASA NTRS1984

Temporal Variations of Solar UV Spectral Irradiance Caused by Solar Rotation and Active Region Evolution

Variations in the solar 100 to 400 nm UV spectral irradiance caused by solar rotation and active region evolution, are discussed as a function of UV wavelength, CMD dependence, and in relation to the temporal variations in the total solar irradiance, 10.7 cm radio flux, sunspot number and Ca K plage data. Active region radiation at cm wavelengths includes a component proportional to the magnetic field. Active region evolution involves a more rapid growth, peak and decay of sunspots and their strong magnetic fields than the Ca K plages and their related UV enhancements. Major plages often last a rotation or more longer than the active region's sunspots. Large active regions, including those associated with major dips in the total solar irradiance, tend to produce the strongest peaks in 10.7 cm and sunspot numbers on their first rotation, while the Ca K plages and UV enhancements peak on the next rotation and decay more slowly on subsequent rotations. Differences in CMD dependencies cause temporal differences including the stronger presence of 13 day variations in the UV flux.

Donnelly, R. F.↗

NASA NTRS1991

Partial purification and characterization of a Ca(2+)-dependent protein kinase from pea nuclei

Almost all the Ca(2+)-dependent protein kinase activity in nuclei purified from etiolated pea (Pisum sativum, L.) plumules is present in a single enzyme that can be extracted from chromatin by 0.3 molar NaCl. This protein kinase can be further purified 80,000-fold by salt fractionation and high performance liquid chromatography, after which it has a high specific activity of about 100 picomoles per minute per microgram in the presence of Ca2+ and reaches half-maximal activation at about 3 x 10(-7) molar free Ca2+, without calmodulin. It is a monomer with a molecular weight near 90,000. It can efficiently use histone III-S, ribosomal S6 protein, and casein as artificial substrates, but it phosphorylates phosvitin only weakly. Its Ca(2+)-dependent kinase activity is half-maximally inhibited by 0.1 millimolar chlorpromazine, by 35 nanomolar K-252a and by 7 nanomolar staurosporine. It is insensitive to sphingosine, an inhibitor of protein kinase C, and to basic polypeptides that block other Ca(2+)-dependent protein kinases. It is not stimulated by exogenous phospholipids or fatty acids. In intact isolated pea nuclei it preferentially phosphorylates several chromatin-associated proteins, with the most phosphorylated protein band being near the same molecular weight (43,000) as a nuclear protein substrate whose phosphorylation has been reported to be stimulated by phytochrome in a calcium-dependent fashion.

NASA Discipline Number 40-50↗

NASA NTRS1983

An H-alpha survey of southern hemisphere active chromosphere stars

Because of the variety of extraordinary phenomena exhibited by active chromosphere objects, discovery of new, bright surface-active stars is of considerable importance. Ca II emission is a well-known signature of chromospheric activity, serving even as one of the points of definition of the class of RS CVn binary stars. In connection with the present investigation, spectroscopic observations of 27 Ca II emission stars have been conducted. The observations make it possible to identify unambiguously the most chromospherically active stars in the sample. By observing the H-alpha line, rather than H and K, it is possible to distinguish nine of these stars which are likely to be observational targets as interesting as the extremely surface active objects V711 Tau or FK Com. Of the 27 stars surveyed, two (HD 86005, HD 204128) showed H-alpha as an emission feature above continuum, with estimated equivalent width 1-2 A.

Bopp, B. W.↗

NASA NTRS1993

The chromospheres of late-type stars. II - An atlas of chromospheric lines for selected early-K stars

High-resolution spectra of the chromospheric Na I D lines, Ca I 4227, Mg I 4571, 5167, and 5172, the Ca II H and K resonance and IR 8542.144 and 8662.170 lines, and H-alpha and H-beta, all observed simultaneously at the AAT, are presented. These data are presented as the observational basis for the self-consistent, semiempirical modeling of the outer photospheres and chromospheres of the target stars. Stellar activity is found in the low-chromosphere lines as core-filling and, in some stars, as line-broadening. Integrated fluxes are derived from the difference spectra, formed by subtracting quiet from active spectra, in the Ca II H and K resonance and IR 8542 and 8662 lines and in H-alpha, which are presented as chromospheric activity indicators. All the activity indices exhibit strong correlations, with the exception of the equivalent widths of H-alpha and H-beta, which are found to be relatively poor activity discriminators.

Thatcher, John D.↗

NASA NTRS1987

Chromospheric activity in open clusters

Spectra of Ca II H and K are being taken for stars of similar mass in the Hyades, the Pleiades, and NGC 752. These spectra will be used to create indices of chromospheric activity on these stars. The dispersion in these indices will then be compared to model dispersions which take into account stellar inclination, position of active regions, and filling factor. Only a few observations have been made to date. These show that a high signal to noise is achievable over reasonable exposure times. Modeling has only just begun.

Pilger, E. J.↗

NASA NTRS2003

Effect Of Simulated Microgravity On Activated T Cell Gene Transcription

Studies of T lymphocytes under the shear stress environment of clinorotation have demonstrated an inhibition of activation in response to TCR mediated signaling. These results mimic those observed during space flight. This work investigates the molecular signaling events of T lymphocyte activation with clinorotation. Purified human T lymphocytes and the T cell clone Jurkat exhibit an uncoupling of signaling as mediated through the TCR. Activation of the transcription factor AP-1 is inhibited while activation of NFAT occurs. NFAT dephosphorylation and activation is dependent on sustained Ca(++) influx. Alternatively, AP-1, which consists of two transcription factors, jun and fos, is activated by PKC and Ras mediated pathways. TCR signaling is known to be dependent on cytoskeletal rearrangements, in particular, raft aggregation is critical. Raft aggregation, as mediated through GM, crosslinking, overcomes the inhibition of T lymphocyte activation with clinorotation, indicating that the block is occurring upstream of raft aggregation. Clinorotation is shown to have an effect similar to a weak TCR signal.

Morrow, Maureen A.↗

NASA NTRS2003

Ising model of cardiac thin filament activation with nearest-neighbor cooperative interactions

We have developed a model of cardiac thin filament activation using an Ising model approach from equilibrium statistical physics. This model explicitly represents nearest-neighbor interactions between 26 troponin/tropomyosin units along a one-dimensional array that represents the cardiac thin filament. With transition rates chosen to match experimental data, the results show that the resulting force-pCa (F-pCa) relations are similar to Hill functions with asymmetries, as seen in experimental data. Specifically, Hill plots showing (log(F/(1-F)) vs. log [Ca]) reveal a steeper slope below the half activation point (Ca(50)) compared with above. Parameter variation studies show interplay of parameters that affect the apparent cooperativity and asymmetry in the F-pCa relations. The model also predicts that Ca binding is uncooperative for low [Ca], becomes steeper near Ca(50), and becomes uncooperative again at higher [Ca]. The steepness near Ca(50) mirrors the steep F-pCa as a result of thermodynamic considerations. The model also predicts that the correlation between troponin/tropomyosin units along the one-dimensional array quickly decays at high and low [Ca], but near Ca(50), high correlation occurs across the whole array. This work provides a simple model that can account for the steepness and shape of F-pCa relations that other models fail to reproduce.

Evaluation Studies↗

NASA NTRS1999

Effect of Space Flight on Adrenal Medullary Function

We hypothesize that microgravity conditions during space flight alter the expression and specific activities of the adrenal medullary CA synthesizing enzymes (CASE). Previously, we examined adrenals from six rats flown for six days aboard STS 54 and reported that microgravity induced a decrease in the expression and specific activity of rat adrenal medullary tyrosine hydroxylase, the rate limiting enzyme of CA synthesis, without affecting the expression of other CASE. In the past, we analyzed some of the > 300 adrenals from two previous Space Shuttle missions (PARE 03 and SLS 2). The preliminary results (a) attest to the good state of tissue preservation, thus proving the feasibility of subsequent large-scale evaluation, and (b) confirm and extend our previous findings. With this grant we will be able to expeditiously analyze all our specimens and to complete our studies in a timely fashion.

Lelkes, Peter I.↗

NASA NTRS1990

Catalysis of carbon monoxide methanation by deep sea manganate minerals

The catalytic activity of deep sea manganese nodule minerals for the methanation of carbon monoxide was measured with a microcatalytic technique between 200 and 460 degrees C. The manganate minerals were activated at 248 degrees C by immersion into a stream of hydrogen in which pulses of carbon monoxide were injected. Activation energies for the methanation reaction and hydrogen desorption from the manganate minerals were obtained and compared with those of pure nickel. Similar energy values indicate that the activity of the nodule materials for the reaction appears to be related to the amount of reducible transition metals present in the samples (ca. 11 wt.-%). Since the activity of the nodule minerals per gram is comparable to that of pure nickel, most of the transition metal ions located between manganese oxide layers appear to be exposed and available to catalyze the reaction.

Non-NASA Center↗

NASA NTRS1995

Neutron-capture Cl-36, Ca-41, Ar-36, and Sm-150 in large chondrites: Evidence for high fluences of thermalized neutrons

We have measured significant concentrations of Cl-36, Ca-41, Ar-36 from decay of Cl-36, and Sm-150 produced from the capture of thermalized neutrons in the large Chico L6 chondrite. Activities of Cl-36 and Ca-41, corrected for a high-energy spallogenic component and a terrestrial age of approximately 50 ka, give average neutron-capture production rates of 208 atoms/min/g-Cl and 1525 atoms/min/kg-Ca, which correspond to thermal neutron (n) fluxes of 6.2 n/sq cm/s and 4.3 n/sq cm/s, respectively. If sustained for the approximately 65 Ma single-stage, cosmic ray exposure age of Chico, these values correspond to thermal neutron fluences of approximately 1.3 x 10(exp 16) and 0.8 x 10(exp 16) n/sq cm for Cl-36 and Ca-41, respectively. Stepwise temperature extraction of Ar in Chico impact melt shows Ar-36/Ar-38 ratios as large as approximately 9. The correlation of high Ar-36/Ar-38 with high Cl/Ca phases in neutron-irradiated Chico indicates that the excess Ar-36 above that expected from spallation is due to decay of neutron-produced Cl-36. Excess Ar-36 in Chico requires a thermal neutron fluence of 0.9-1.7 x 10(exp 16) n/sq cm. Decreases in Sm-149/Sm-152 due to neutron-capture by Sm-149 correlate with increases in Sm-150/Sm-152 for three samples of Chico, and one of the Torino H-chondrite. The 0.08% decrease in Sm-149 shown by Chico corresponds to a neutron fluence of 1.23 x 10(exp 16) n/sq cm. This fluence derived from Sm considers capture of epithermal neutrons and effects of chemical composition on the neutron energy distribution. Excess Ar-36 identified in the Arapahoe, Bruderheim, and Torino chondrites and the Shallowater aubrite suggest exposure to neutron fluences of approximately 0.2-0.2 x 10(exp 16) n/sq cm. Depletion of Sm-149 in Torino and the LEW86010 angrite suggest neutron fluences of 0.8 x 10(exp 16) n/sq cm and 0.25 x 10(exp 16) n/sq cm, respectively. Neutron fluences of approximately 10(exp 16) n/sq cm in Chico are almost as large as those previously observed for some lunar soils. Consideration of exposure ages suggests that the neutron flux in Chico may have been greater than that in many lunar soils.

Bogard, D. D.↗

NASA NTRS1981

Coronal loops and active region structure

Synoptic H-alpha Ca K, magnetograph and Skylab soft X-ray and EUV data were compared for the purpose of identifying the basic coronal magnetic structure of loops in a 'typical' active region and studying its evolution. A complex of activity in July 1973, especially McMath 12417, was emphasized. The principal results are: (1) most of the brightest loops connected the bright f plage to either the sunspot penumbra or to p satellite spots; no non-flaring X-ray loops end in umbrae; (2) short, bright loops had one or both ends in regions of emergent flux, strong field or high field gradients; (3) stable, strongly sheared loop arcades formed over filaments; (4) EFRs were always associated with compact X-ray arcades; and (5) loops connecting to other active regions had their bases in outlying plage of weak field strength in McM 417 where H-alpha fibrils marked the direction of the loops

Webb, D. F.↗