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At least 55 records · Page 3

Metatranscriptomic Sequencing of Winter and Spring Planktonic Communities from Lake Erie, a Laurentian Great Lake

Previous reports suggest planktonic and under-ice winter microbial communities in Lake Erie are dominated by diatoms. Here, we report the assembled metatranscriptomes of 79 Lake Erie surface water microbial communities spanning both the winter (28 samples) and spring (51 samples) months over spatial, temporal, and climatic gradients in 2019 through 2020.

Zepernick, Brittany N.↗

Seasonal Patterns and Remote Spectral Estimation of Canopy Chemistry Across the Oregon Transect

We examined seasonal changes in canopy chemical concentrations and content in conifer forests growing along a climate gradient in western Oregon, as part of the Oregon Transect Ecosystem Research (OTTER) study. The chemical variables were related to seasonal patterns of growth and production. Statistical comparisons of chemical variables with data collected from two different airborne remote-sensing platforms were also carried out. Total nitrogen (N) concentrations in foliage varied significantly both seasonally and among sites; when expressed as content in the forest canopy, nitrogen varied to a much greater extent and was significantly related to aboveground net primary production (r = 0.99). Chlorophyll and free amino acid concentrations varied more strongly than did total N and may have reflected changes in physiological demands for N. Large variations in starch concentrations were measured from pre- to post-budbreak in all conifer sites. Examination of remote-sensing data from two different airborne instruments suggests the potential for remote measurement of some canopy chemicals. Multivariate analysis of high-resolution spectral data in the near infrared region indicated significant correlations between spectral signals and N concentration and canopy N content; the correlation with canopy N content was stronger and was probably associated in part with water absorption features of the forest canopy. The spectral bands that were significantly correlated with lignin concentration and content were similar to bands selected in the other laboratory and airborne studies; starch concentrations were not significantly related to spectral reflectance data. Strong relationships between the spectral position of specific reflectance features in the visible region and chlorophyll were also found.

Matson, Pamela↗

Mapping Global Forest Age from Forest Inventories, Biomass and Climate Data

Forest age can determine the capacity of a forest to uptake carbon from the atmosphere. However, a lack of global diagnostics that reflect the forest stage and associated disturbance regimes hampers the quantification of age-related differences in forest carbon dynamics. This study provides a new global distribution of forest age circa 2010, estimated using a machine learning approach trained with more than 40 000 plots using forest inventory, biomass and climate data. First, an evaluation against the plot-level measurements of forest age reveals that the data-driven method has a relatively good predictive capacity of classifying old-growth vs. non-old-growth (precision = 0.81 and 0.99 for old-growth and non-old-growth, respectively) forests and estimating corresponding forest age estimates (NSE = 0.6 – Nash–Sutcliffe efficiency – and RMSE = 50 years – root-mean-square error). However, there are systematic biases of overestimation in young- and underestimation in old-forest stands, respectively. Globally, we find a large variability in forest age with the old-growth forests in the tropical regions of Amazon and Congo, young forests in China, and intermediate stands in Europe. Furthermore, we find that the regions with high rates of deforestation or forest degradation (e.g. the arc of deforestation in the Amazon) are composed mainly of younger stands. Assessment of forest age in the climate space shows that the old forests are either in cold and dry regions or warm and wet regions, while young–intermediate forests span a large climatic gradient. Finally, comparing the presented forest age estimates with a series of regional products reveals differences rooted in different approaches and different in situ observations and global-scale products. Despite showing robustness in cross-validation results, additional methodological insights on further developments should as much as possible harmonize data across the different approaches. The forest age dataset presented here provides additional insights into the global distribution of forest age to better understand the global dynamics in the forest water and carbon cycles. The forest age datasets are openly available at https://doi.org/10.17871/ForestAgeBGI.2021 (Besnard et al., 2021).

Simon Besnard↗

Arctic Shrub Expansion, Plant Functional Trait Variation, and Effects on Belowground Carbon Cycling (Final Technical Report)

Terrestrial ecosystems are undergoing dramatic changes in response to climate warming, and these changes are expected to feedback to the atmosphere, potentially altering the trajectory of future climate change. Feedbacks from Arctic ecosystems are a major concern because the Arctic is projected to warm significantly in the 21 st century and because >50% of global belowground organic carbon is stored in permafrost and overlying soils. Warming-driven release of this carbon could drastically increase atmospheric greenhouse gas concentrations and accelerate climate warming. Plant communities are also responding to warming, as evidenced by the widely documented increase in woody-shrub growth and “greening” across much of the Arctic tundra biome. This vegetation shift may offset or amplify warming by altering carbon cycling. The direction and magnitude of shrub effects remain highly uncertain, however, due to limited understanding of the consequences of shrub expansion for belowground carbon cycling and simplification of these relationships in models. The major shrubs expanding in the Arctic (Betula, Salix, and Alnus) vary widely with respect to aboveground and belowground traits (e.g., tissue production and chemistry, rooting depth, microbial symbionts), and may also exhibit substantial intraspecific variation in these traits in response to environmental conditions. Such variation is likely to have profound implications for soil carbon cycling. The overarching goal of this project was to improve process-based understanding of the influence of shrub expansion on carbon cycling to enable improved representation of carbon dynamics in ecosystem and Earth system models. We investigated how plant functional traits vary among shrub genera, respond to environmental conditions, and affect belowground carbon and nutrient cycling by quantifying relationships among functional traits and biogeochemical cycling along edaphic gradients nested within a climate gradient in the Alaskan tundra. We found consistent differences in leaf and root traits among shrub genera and between shrubs and a widespread sedge species, indicating diverse nutrient acquisition strategies and belowground impacts among different arctic shrubs. We also found striking differences in trait values among individuals within the same species or genera within sites. Soil parameters were more important than climate parameters for predicting size and leaf trait variation, and root trait responses were less dependent on climate overall. For all but one root trait, including parameters representing aboveground traits improved the predictive ability of models. These results demonstrate that tundra shrub traits vary considerably at local scales and soil factors drive this variation, especially belowground. Furthermore, leveraging information about aboveground traits and soil conditions can improve predictions of how belowground traits will respond to climate change. Despite these differences, soil carbon and nitrogen pools in the active layer did not vary among plots dominated by different shrub or sedge genera. Instead, pool sizes generally decreased from warmer to colder sites, consistent with a productivity gradient. Patterns of isotopic N composition indicate that shrubs tighten nitrogen cycling via nitrogen resorption or immobilization of shrub litter. Overall, these results suggest that further identifying the specific shrub genera in the tundra landscape will ultimately provide better predictions of belowground dynamics across the changing arctic. We also performed simulation experiments with the Terrestrial Ecosystem Model (TEM) incorporated in the Predictive Ecosystem Analyzer (PEcAn) framework, treats model parameters as probability distributions, estimates parameters based on a synthesis of available field data, and then quantifies both model sensitivity and uncertainty to a given parameter or suite of parameters. We performed simulations across different types of tundra, including shrub tundra. One key finding was that both model sensitivity and uncertainty to a given parameter could vary within the same type of tundra, but in a different geographical location, such as over the climate gradient of shrub tundra described above. We organized a special session at the annual meeting of the Ecological Society of America in August 2019 to disseminate our results, refine recommendations for model improvement, and initiate collaborations to implement these recommendations in existing models of tundra carbon dynamics at ecosystem to Earth system scales. Our results support DOE near-term priorities by providing mechanistic insights into the role of vegetation change in the terrestrial carbon cycle in a region that is inadequately represented in Earth system models. Current models reduce the complexity of Arctic vegetation to a small number of plant functional types (PFTs). This approach implicitly assumes that each PFT represents the average ecological function of its constituent species, thus ignoring the effects of trait variation on biogeochemical cycling and potentially leading to large uncertainty in the sign and magnitude of ecosystem feedbacks to climate. By quantifying variation of plant functional traits across broad gradients of climatic and edaphic conditions and elucidating the linkages of such variation with carbon and nutrient cycling, our results illustrate the need and create a foundation for further developing trait-based modeling approaches that allow the traits of PFTs to vary as a function of environmental conditions. These approaches should improve the capacity of simulation models to offer insights into ecosystem carbon dynamics associated with novel plant communities in a rapidly changing Arctic.

54 ENVIRONMENTAL SCIENCES↗

Large carbon cycle sensitivities to climate across a permafrost thaw gradient in subarctic Sweden

Permafrost peatlands store large amounts of carbon potentially vulnerable to decomposition. However, the fate of that carbon in a changing climate remains uncertain in models due to complex interactions among hydrological, biogeochemical, microbial, and plant processes. In this study, we estimated effects of climate forcing biases present in global climate reanalysis products on carbon cycle predictions at a thawing permafrost peatland in subarctic Sweden. The analysis was conducted with a comprehensive biogeochemical model ( ecosys ) across a permafrost thaw gradient encompassing intact permafrost palsa with an ice core and a shallow active layer, partly thawed bog with a deeper active layer and a variable water table, and fen with a water table close to the surface, each with distinct vegetation and microbiota. Using in situ observations to correct local cold and wet biases found in the Global Soil Wetness Project Phase 3 (GSWP3) climate reanalysis forcing, we demonstrate good model performance by comparing predicted and observed carbon dioxide (CO 2 ) and methane (CH 4 ) exchanges, thaw depth, and water table depth. The simulations driven by the bias-corrected climate suggest that the three peatland types currently accumulate carbon from the atmosphere, although the bog and fen sites can have annual positive radiative forcing impacts due to their higher CH 4 emissions. Our simulations indicate that projected precipitation increases could accelerate CH 4 emissions from the palsa area, even without further degradation of palsa permafrost. The GSWP3 cold and wet biases for this site significantly alter simulation results and lead to erroneous active layer depth (ALD) and carbon budget estimates. Biases in simulated CO 2 and CH 4 exchanges from biased climate forcing are as large as those among the thaw stages themselves at a landscape scale across the examined permafrost thaw gradient. Future studies should thus not only focus on changes in carbon budget associated with morphological changes in thawing permafrost, but also recognize the effects of climate forcing uncertainty on carbon cycling.

54 ENVIRONMENTAL SCIENCES↗

A gradient model of vegetation and climate utilizing NOAA satellite imagery. Phase 1: Texas transect

A climatological model/variable termed the sponge (a measure of moisture availability based on daily temperature maxima and minima, and precipitation) was tested for potential biogeograhic, ecological, and agro-climatological applications. Results, depicted in tabular and graphic form, suggest that, as generalized climatic index, sponge is particularly appropriate for large-area and global vegetation monitoring. The feasibility of utilizing NOAA/AVHRR data for vegetation classification was investigated and a vegetation gradient model that utilizes sponge and AVHRR data was initiated. Along an east-west Texas gradient, vegetation, sponge, and AVHRR pixel data (channels 1 and 2) were obtained for 12 locations. The normalized difference values for the AVHRR data when plotted against vegetation characteristics (biomass, net productivity, leaf area) and sponge values along the Texas gradient suggest that a multivariate gradient model incorporating AVHRR and sponge data may indeed be useful in global vegetation stratification and monitoring.

Greegor, D.↗

Further adoption of conservation tillage can increase maize yields in the western US Corn Belt

Conservation tillage can reduce soil erosion, increase soil health, and decrease labor and fuel input costs. Despite these benefits, potential yield impacts remain an important concern for farmers considering adoption. Previous research suggests that conservation tillage is likely to have the largest yield benefits in more arid conditions, but a lack of field-level analyses across climatic, management and soil conditions limits confidence in such predictions. Satellite imagery provides the opportunity to monitor agricultural lands at sub-field resolution across large spatial scales and wide environmental gradients. Here we investigate the maize yield impacts of conservation tillage in the semi-arid western US Corn Belt, using sub-field resolution datasets on tillage practices and crop yields derived from satellite data spanning four states (Nebraska, Kansas, South Dakota, and North Dakota) between 2008 and 2020. On these datasets, we estimate heterogenous yield outcomes for several thousand maize fields across gradients in climate, soil quality and irrigation status by using a causal forests analysis, an adaptation of the random forests machine-learning algorithm for causal inference on observational data. We find that long-term adoption of conservation tillage increased rainfed maize yields by an average of 9.9% in the region. Impacts on irrigated yields were small and not statistically significant. These results, along with an analysis of variables related to greater than average yield benefits, indicate that improved water infiltration and retention are the primary reasons for conservation tillage benefits. Despite yield benefits, many fields estimated to see increased yields under long term low till have not adopted the practice. Therefore, we identify specific counties likely to benefit most from increased levels of adoption. Our results strengthen the understanding of the impacts of conservation agriculture on crop yields and help define environments and counties most likely to benefit from conservation tillage.

54 ENVIRONMENTAL SCIENCES↗

A gradient model of vegetation and climate utilizing NOAA satellite imagery. Phase 1: Texas transect

A new experimental climatological model/variable termed the sponge, a measure of moisture availability based on daily temperature maxima and minima and precipitation, is tested for potential biogeographic, ecological, and agro-climatological applications. Results, depicted in tabular and graphic from, suggest that, as a generalized climatic index, sponge's simplicity and sensitivity make particularly appropriate for trans-regional biogeographic studies (e.g., large-area and global vegetation monitoring). The feasibility of utilizing NOAA/AVHRR data for vegetation classification was investigated and a vegetation gradient model that utilizes sponge, and AVHRR pixel data (channels 1 and 2) were obtained for 12 locations. The normalized difference values for the AVHRR data when plotted against vegetation characteristics (biomass, net productivity, leaf area) and sponge values suggest that a multivariate gradient model incorporating AVHRR and sponge data may indeed be useful in global vegetation stratification and monitoring.

Greegor, D. H.↗

Unraveling the effects of management and climate on carbon fluxes of U.S. croplands using the USDA Long-Term Agroecosystem (LTAR) network

Understanding the carbon fluxes and dynamics from a broad range of agricultural systems has the potential to improve our ability to increase carbon sequestration while maintaining crop yields. Short-term, single-location studies have limited applicability, but long-term data from a network of many locations can provide a broader understanding across gradients of climate and management choices. Here we examine eddy covariance measured carbon dioxide (CO 2 ) fluxes from cropland sites across the United States Department of Agriculture's Long-Term Agroecosystem Research (LTAR) network. The dataset was collected between 2001 and 2020, spanning 13 sites for a total of 182 site-years. Average seasonal patterns of net ecosystem CO 2 exchange (NEE), gross primary productivity (GPP), and ecosystem respiration (R eco ) were determined, and subsequent regression analysis on these “flux climatologies” was used to identify relationships to mean annual temperature (MAT), mean annual precipitation (MAP), cropping systems, and management practices. At rainfed sites, carbon fluxes were better correlated with MAP (r2 ≤ 0.5) than MAT (r2 ≤ 0.22). Net carbon balance was different among cropping systems (p < 0.001), with the greatest net carbon uptake occurring in sugarcane (Saccharum spp. hybrids) and the least in soybean (Glycine max) fields. Crop type had a greater effect on carbon balance than irrigation management at a Nebraska site. Across cropping systems, grain crops often had higher GPP and were more likely to have net uptake when compared to legume crops. This multi-site analysis highlights the potential of the LTAR network to further carbon flux research using eddy covariance measurements.

54 ENVIRONMENTAL SCIENCES↗

Harvest Intensity Effects on Carbon Stocks and Biodiversity Are Dependent on Regional Climate in Douglas-Fir Forests of British Columbia

Temperate forests provide crucial ecosystems services as living sinks for atmospheric carbon (C) and repositories of biodiversity. Applying harvesting at intensities that minimize losses offers one means for mitigating global change. However, little is known of overstory retention levels that best conserve ecosystem services in different regional climates, and likewise as climate changes. To quantify the effect of harvest intensity on C stocks and biodiversity, we compared five harvesting intensities (clearcutting, seedtree retention, 30% patch retention, 60% patch retention, and uncut controls) across a climatic aridity gradient that ranged from humid to semi-arid in the Douglas-fir (Pseudotsuga menziesii) forests of British Columbia. We found that increased harvesting intensity reduced total ecosystem, above ground, and live tree C stocks 1 year post-harvest, and the magnitude of these losses were negatively correlated with climatic aridity. In humid forests, total ecosystem C ranged from 50% loss following clearcut harvest, to 30% loss following large patch retention harvest. In arid forests this range was 60 to 8% loss, respectively. Where lower retention harvests are sought, the small patch retention treatment protected both C stocks and biodiversity in the arid forests, whereas the seedtree method performed as well or better in the humid forests. Below ground C stocks declined by an average of 29% after harvesting, with almost all of the loss from the forest floor and none from the mineral soil. Of the secondary pools, standing and coarse deadwood declined in all harvesting treatments regardless of cutting intensity or aridity, while C stocks in fine fuels and stumps increased. The understory plant C pool declined across all harvesting intensities in the humid forests, but increased in arid forests. Shannon’s diversity and richness of tree and bryoid species declined with harvesting intensity, where tree species losses were greatest in the humid forests and bryoid losses greatest in arid forests. Shrub and herb species were unaffected. This study showed that the highest retention level was best at reducing losses in C stocks and biodiversity, and clearcutting the poorest, and while partial retention of canopy trees can reduce losses in these ecosystem services, outcomes will vary with climatic aridity.

54 ENVIRONMENTAL SCIENCES↗

The stability of ground ice in the equatorial region of Mars

The lifetime of an unreplenished layer of ground ice lying within 30 deg of the Martian equator was examined within the context of the existing data base on Martian regolith and climate. Data on the partial pressure of H2O in the Martian atmosphere and the range of mean annual temperatures indicated the ground ice would be restricted to latitudes poleward of 40 deg. However, the ground ice near the poles may be a relic from early Martian geologic times held in place by a thin layer of regolith. Consideration of twelve model pore size distributions, similar to silt- and clay-type earth soils, was combined with a parallel pore model of gaseous diffusion to calculate the flux of H2O molecules escaping from the subsurface ground ice layer. Martian equatorial ground ice was found to be influenced by the soil structure, the magnitude of the geothermal gradient, the climatic desorption of CO2 from the regolith. It is concluded that equatorial ground ice is present on Mars only if a process of replenishment is active.

Clifford, S. M.↗

BioRT-Flux-PIHM v1.0: a biogeochemical reactive transport model at the watershed scale

Watersheds are the fundamental Earth surface functioning units that connect the land to aquatic systems. Many watershed-scale models represent hydrological processes but not biogeochemical reactive transport processes. This has limited our capability to understand and predict solute export, water chemistry and quality, and Earth system response to changing climate and anthropogenic conditions. Here we present a recently developed BioRT-Flux-PIHM (BioRT hereafter) v1.0, a watershed-scale biogeochemical reactive transport model. The model augments the previously developed RT-Flux-PIHM that integrates land-surface interactions, surface hydrology, and abiotic geochemical reactions. It enables the simulation of (1) shallow and deep-water partitioning to represent surface runoff, shallow soil water, and deeper groundwater and of (2) biotic processes including plant uptake, soil respiration, and nutrient transformation. The reactive transport part of the code has been verified against the widely used reactive transport code CrunchTope. BioRT-Flux-PIHM v1.0 has recently been applied in multiple watersheds under diverse climate, vegetation, and geological conditions. This paper briefly introduces the governing equations and model structure with a focus on new aspects of the model. It also showcases one hydrology example that simulates shallow and deep-water interactions and two biogeochemical examples relevant to nitrate and dissolved organic carbon (DOC). These examples are illustrated in two simulation modes of complexity. One is the spatially lumped mode (i.e., two land cells connected by one river segment) that focuses on processes and average behavior of a watershed. Another is the spatially distributed mode (i.e., hundreds of cells) that includes details of topography, land cover, and soil properties. Whereas the spatially lumped mode represents averaged properties and processes and temporal variations, the spatially distributed mode can be used to understand the impacts of spatial structure and identify hot spots of biogeochemical reactions. The model can be used to mechanistically understand coupled hydrological and biogeochemical processes under gradients of climate, vegetation, geology, and land use conditions.

54 ENVIRONMENTAL SCIENCES↗

Linking Dissolved Organic Matter Composition to Landscape Properties in Wetlands Across the United States of America

Abstract Wetlands are integral to the global carbon cycle, serving as both a source and a sink for organic carbon. Their potential for carbon storage will likely change in the coming decades in response to higher temperatures and variable precipitation patterns. We characterized the dissolved organic carbon (DOC) and dissolved organic matter (DOM) composition from 12 different wetland sites across the USA spanning gradients in climate, landcover, sampling depth, and hydroperiod for comparison to DOM in other inland waters. Using absorption spectroscopy, parallel factor analysis modeling, and ultra‐high resolution mass spectroscopy, we identified differences in DOM sourcing and processing by geographic site. Wetland DOM composition was driven primarily by differences in landcover where forested sites contained greater aromatic and oxygenated DOM content compared to grassland/herbaceous sites which were more aliphatic and enriched in N and S molecular formulae. Furthermore, surface and porewater DOM was also influenced by properties such as soil type, organic matter content, and precipitation. Surface water DOM was relatively enriched in oxygenated higher molecular weight formulae representing HUP High O/C compounds than porewaters, whose DOM composition suggests abiotic sulfurization from dissolved inorganic sulfide. Finally, we identified a group of persistent molecular formulae (3,489) present across all sites and sampling depths (i.e., the signature of wetland DOM) that are likely important for riverine‐to‐coastal DOM transport. As anthropogenic disturbances continue to impact temperate wetlands, this study highlights drivers of DOM composition fundamental for understanding how wetland organic carbon will change, and thus its role in biogeochemical cycling.

Environmental Sciences & Ecology↗

Optimizing process-based models to predict current and future soil organic carbon stocks at high-resolution

From hillslope to small catchment scales (< 50 km 2 ), soil carbon management and mitigation policies rely on estimates and projections of soil organic carbon (SOC) stocks. Here we apply a process-based modeling approach that parameterizes the MIcrobial-MIneral Carbon Stabilization (MIMICS) model with SOC measurements and remotely sensed environmental data from the Reynolds Creek Experimental Watershed in SW Idaho, USA. Calibrating model parameters reduced error between simulated and observed SOC stocks by 25%, relative to the initial parameter estimates and better captured local gradients in climate and productivity. The calibrated parameter ensemble was used to produce spatially continuous, high-resolution (10 m 2 ) estimates of stocks and associated uncertainties of litter, microbial biomass, particulate, and protected SOC pools across the complex landscape. Here, subsequent projections of SOC response to idealized environmental disturbances illustrate the spatial complexity of potential SOC vulnerabilities across the watershed. Parametric uncertainty generated physicochemically protected soil C stocks that varied by a mean factor of 4.4 × across individual locations in the watershed and a – 14.9 to + 20.4% range in potential SOC stock response to idealized disturbances, illustrating the need for additional measurements of soil carbon fractions and their turnover time to improve confidence in the MIMICS simulations of SOC dynamics.

54 ENVIRONMENTAL SCIENCES↗

Decomposition decreases molecular diversity and ecosystem similarity of soil organic matter

Soil organic matter (SOM) is comprised of a diverse array of reactive carbon molecules, including hydrophilic and hydrophobic compounds, that impact rates of SOM formation and persistence. Despite clear importance to ecosystem science, little is known about broad-scale controls on SOM diversity and variability in soil. Here, we show that microbial decomposition drives significant variability in the molecular richness and diversity of SOM between soil horizons and across a continental-scale gradient in climate and ecosystem type (arid shrubs, coniferous, deciduous, and mixed forests, grasslands, and tundra sedges). The molecular dissimilarity of SOM was strongly influenced by ecosystem type (hydrophilic compounds: 17%, P < 0.001; hydrophobic compounds: 10% P < 0.001) and soil horizon (hydrophilic compounds: 17%, P < 0.001; hydrophobic compounds: 21%, P < 0.001), as assessed using metabolomic analysis of hydrophilic and hydrophobic metabolites. While the proportion of shared molecular features was significantly higher in the litter layer than subsoil C horizons across ecosystems (12 times and 4 times higher for hydrophilic and hydrophobic compounds, respectively), the proportion of site-specific molecular features nearly doubled from the litter layer to the subsoil horizon, suggesting greater differentiation of compounds after microbial decomposition within each ecosystem. Together, these results suggest that microbial decomposition of plant litter leads to a decrease in SOM α-molecular diversity, yet an increase in β-molecular diversity across ecosystems. The degree of microbial degradation, determined by the position in the soil profile, exerts a greater control on SOM molecular diversity than environmental factors, such as soil texture, moisture, and ecosystem type.

59 BASIC BIOLOGICAL SCIENCES↗

Roots Mediate the Effects of Snowpack Decline on Soil Bacteria, Fungi, and Nitrogen Cycling in a Northern Hardwood Forest

Rising winter air temperature will reduce snow depth and duration over the next century in northern hardwood forests. Reductions in snow depth may affect soil bacteria and fungi directly, but also affect soil microbes indirectly through effects of snowpack loss on plant roots. We incubated root exclusion and root ingrowth cores across a winter climate-elevation gradient in a northern hardwood forest for 29 months to identify direct (i.e., winter snow-mediated) and indirect (i.e., root-mediated) effects of winter snowpack decline on soil bacterial and fungal communities, as well as on potential nitrification and net N mineralization rates. Both winter snowpack decline and root exclusion increased bacterial richness and phylogenetic diversity. Variation in bacterial community composition was best explained by differences in winter snow depth or soil frost across elevation. Root ingrowth had a positive effect on the relative abundance of several bacterial taxonomic orders (e.g., Acidobacterales and Actinomycetales). Nominally saprotrophic (e.g., Saccharomycetales and Mucorales) or mycorrhizal (e.g., Helotiales, Russalales, Thelephorales) fungal taxonomic orders were also affected by both root ingrowth and snow depth variation. However, when grouped together, the relative abundance of saprotrophic fungi, arbuscular mycorrhizal fungi, and ectomycorrhizal fungi were not affected by root ingrowth or snow depth, suggesting that traits in addition to trophic mode will mediate fungal community responses to snowpack decline in northern hardwood forests. Potential soil nitrification rates were positively related to ammonia-oxidizing bacteria and archaea abundance (e.g., Nitrospirales, Nitrosomondales, Nitrosphaerales). Rates of N mineralization were positively and negatively correlated with ectomycorrhizal and saprotrophic fungi, respectively, and these relationships were mediated by root exclusion. The results from this study suggest that a declining winter snowpack and its effect on plant roots each have direct effects on the diversity and abundance of soil bacteria and fungal communities that interact to determine rates of soil N cycling in northern hardwood forests.

54 ENVIRONMENTAL SCIENCES↗

Space Based Ornithology: On the Wings of Migration and Biophysics

The study of bird migration on a global scale is one of the compelling and challenging problems of modern biology with major implications for human health and conservation biology. Migration and conservation efforts cross national boundaries and are subject to numerous international agreements and treaties. Space based technology offers new opportunities to shed understanding on the distribution and migration of organisms on the planet and their sensitivity to human disturbances and environmental changes. Migration is an incredibly diverse and complex behavior. A broad outline of space based research must address three fundamental questions: (1) where could birds be, i.e. what is their fundamental niche constrained by their biophysical limits? (2) where do we actually find birds, i.e. what is their realizable niche as modified by local or regional abiotic and biotic factors, and (3) how do they get there (and how do we know?), that is what are their migration patterns and associated mechanisms? Our working hypothesis is that individual organism biophysical models of energy and water balance, driven by satellite measurements of spatio-temporal gradients in climate and habitat, will help us to explain the variability in avian species richness and distribution. Dynamic state variable modeling provides one tool for studying bird migration across multiple scales and can be linked to mechanistic models describing the time and energy budget states of migrating birds. Such models yield an understanding of how a migratory flyway and its component habitats function as a whole and link stop-over ecology with biological conservation and management. Further these models provide an ecological forecasting tool for science and application users to address what are the possible consequences of loss of wetlands, flooding, drought or other natural disasters such as hurricanes on avian biodiversity and bird migration.

Smith, James A.↗

Space-based Remote Sensing: A Tool for Studying Bird Migration Across Multiple Scales

The study of bird migration on a global scale is one of the compelling and challenging problems of modern biology with major implications for human health and conservation biology. Migration and conservation efforts cross national boundaries and are subject to numerous international agreements and treaties. Space based technology offers new opportunities to shed understanding on the distribution and migration of organisms on the planet and their sensitivity to human disturbances and environmental changes. Our working hypothesis is that individual organism biophysical models of energy and water balance, driven by satellite measurements of spatio-temporal gradients in climate and habitat, will help us to explain the variability in avian species richness and distribution. Further, these models provide an ecological forecasting tool for science and application users to visualize the possible consequences of loss of wetlands, flooding, or other natural disasters such as hurricanes on avian biodiversity and bird migration.

Smith, James A.↗