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At least 55 records · Page 3

Plan evaluation for heat resilience: complementary methods to comprehensively assess heat planning in Tempe and Tucson, Arizona

Abstract Escalating impacts from climate change and urban heat are increasing the urgency for communities to equitably plan for heat resilience. Cities in the desert Southwest are among the hottest and fastest warming in the U.S., placing them on the front lines of heat planning. Urban heat resilience requires an integrated planning approach that coordinates strategies across the network of plans that shape the built environment and risk patterns. To date, few studies have assessed cities’ progress on heat planning. This research is the first to combine two emerging plan evaluation approaches to examine how networks of plans shape urban heat resilience through case studies of Tempe and Tucson, Arizona. The first methodology, Plan Quality Evaluation for Heat Resilience, adapts existing plan quality assessment approaches to heat. We assess whether plans meet 56 criteria across seven principles of high-quality planning and the types of heat strategies included in the plans. The second methodology, the Plan Integration for Resilience Scorecard™ (PIRS™) for Heat, focuses on plan policies that could influence urban heat hazards. We categorize policies by policy tool and heat mitigation strategy and score them based on their heat impact. Scored policies are then mapped to evaluate their spatial distribution and the net effect of the plan network. The resulting PIRS™ for Heat scorecard is compared with heat vulnerability indicators to assess policy alignment with risks. We find that both cities are proactively planning for heat resilience using similar plan and strategy types, however, there are clear and consistent opportunities for improvement. Combining these complementary plan evaluation methods provides a more comprehensive understanding of how plans address heat and a generalizable approach that communities everywhere could use to identify opportunities for improved heat resilience planning.

Environmental Sciences & Ecology↗

Temperature, Humidity, and Time-Lapse Video Data from the East River Watershed, Water Year 2024

A new version of this dataset is available at doi:10.15485/3001338 and is the first citation in the 'Related References' section. It is expands on this dataset by appending another water year of data collection and additional logger sites.This dataset contains time-lapse imagery and distributed measurements of air temperature, relative humidity, dew point, and soil temperature across the East River basin from 3 October 2023 to 12 August 2024. Instruments were deployed at 14 sites as part of the DOE Grant: Seasonal Cycles Unravel Mysteries of Missing Mountain Water organized by Jessica Lundquist (University of Washington), Rosemary Carroll (Desert Research Institute), and Ethan Gutmann (National Center for Atmospheric Research). The data are published to support studies of surface climate or hydrologic processes in complex terrain. Measurements were collected with low-cost data loggers installed 2 m high on evergreen trees or buried just below the soil surface. Time-lapse cameras were deployed at three sites. Imagery from sites AP BONUS and AP5 provides insight into large-scale seasonal snow cover variability. Imagery from site EL2 shows smaller-scale snow patterns across a nearby meadow.Dataset files are organized by site and variable (air measurements, ground measurements, or time-lapse video). Air and ground measurements are packaged in LoggerData.zip, and time-lapse imagery is compiled into short videos stored in TimelapseVideos.zip. File-level metadata contains details for each file included in the dataset. A data dictionary provides units and descriptions for column or row names in all files. The locations metadata file describes site characteristics, locations, and associated GPS methods.Dataset update 2025-03-03: Resolved header and datetime formatting inconsistencies within LoggerData.zip files KP1_Air, KP3_Air, KP3_Ground, KP6_Ground, AP3_Ground, AP4_Air, and AP6_Air.Dataset update 2025-11-18: Modified abstract and related references sections to include new version of dataset.

54 ENVIRONMENTAL SCIENCES↗

Connecting the Radiative Influences of Aerosol upon the Mass Flux Profiles of Shallow Cumuli across the Southeast Atlantic Ocean Basin and its Boundaries (Final Report)

The Atlantic Ocean covers approximately 25% of Earth’s surface and the atmosphere above it is home to a complex array of clouds and aerosols that have important influences on regional and global weather and climate. These influences must be accurately depicted in short range, medium range, seasonal, and climate forecast models. Conditions over the tropical Atlantic are particularly complex due to continental scale plumes of dust from the Sahara Desert and smoke from agricultural burning in Africa that drift across the Atlantic Ocean basin toward the Americas. These plumes are often found meandering in the lower atmosphere above shallow tropical clouds that form above the ocean surface, presumably mingling with these clouds on occasion due to convective mixing processes. Elevated dust and smoke particles absorb incoming sunlight and substantially warm the marine atmosphere in the layer in which they are present. This warming may alter the thermal stability of the marine atmosphere and may throttle or enhance the development of clouds, change their internal structure and the rate at which they precipitate. Alternatively, it may isolate the lower atmosphere from drier layers above enabling water vapor to accumulate near the ocean surface potentially leading to the development of deeper convection. Our study was organized around the principal concept of determining the impact of African smoke and dust plumes upon cloud development at ASI and understanding how a popular shallow convection parameterization used in models responds to the presence of this aerosol. We analyzed observations collected during the US Department of Energy (DOE) Layered Atlantic Smoke Interactions with Clouds (LASIC) campaign using the Atmospheric Radiation Measurement Program’s Mobile Facility #1 (AMF-1), which was deployed to Ascension Island (ASI) in Southeastern Atlantic for a one-year period. Ascension Island is immediately downwind from an African source of these plumes, but far enough removed to enable the lower atmosphere to have reacted to their presence. A main goal of our study was to compute radiative heating rate profiles over Ascension Island and along the trajectory from the biomass burning regions along coastal Africa to Ascension Island. To compute the radiative heating rate due to aerosols and clouds, we employed observed profiles of temperature, humidity, and clouds from LASIC alongside aerosol optical properties from the Modern-era Retrospective analysis for Research and Applications Version 2 (MERRA-2), as input for the Rapid Radiation Transfer Model (RRTM). Radiative heating was also assessed across the southeast Atlantic Ocean using an ensemble of back trajectories from the Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLIT) model. We were successful in this effort and the resulting publication is already being cited despite its relatively short lifetime in the literature. The second part of our study involved the process-level representation of the clouds observed over the Southeastern Atlantic in models. Our initial task, upon which the balance of this portion of the study depended, was to evaluate the representativeness of the clouds observed by AMF-1. What orographic influence did Ascension Island have on the measured cloud structure? We set out to answer this question by trying to separate observations that were clearly indicative of orographic forcing from those that were representative of open ocean. The siting of AMF-1, which was 340-m above sea-level on the slope of a steep escarpment, proved demonstrably problematic for cloud process measurements. Despite considerable effort using multiple approaches including artificial intelligence (AI), we were unable to successfully compensate for the orographic forcing present at the AMF-1 site on ASI and, hence, produce process-level summaries of the convective mass flux representative of open ocean over the Southeastern Atlantic. Even though the project has officially ended, we are making a final attempt using two new types of clustering algorithm (i.e., AI) on the recommendation of a former student, who is an expert in this area. Since we have only recently begun to test these new AI schemes, the recommendations from the second part of our project outlined below are based on our experience at the time of this report.

54 ENVIRONMENTAL SCIENCES↗

Relative importance of high-latitude local and long-range-transported dust for Arctic ice-nucleating particles and impacts on Arctic mixed-phase clouds

Dust particles, serving as ice-nucleating particles (INPs), may impact the Arctic surface energy budget and regional climate by modulating the mixed-phase cloud properties and lifetime. In addition to long-range transport from low-latitude deserts, dust particles in the Arctic can originate from local sources. However, the importance of high-latitude dust (HLD) as a source of Arctic INPs (compared to low-latitude dust, LLD) and its effects on Arctic mixed-phase clouds are overlooked. In this study, we evaluate the contribution to Arctic dust loading and INP population from HLD and six LLD source regions by implementing a source-tagging technique for dust aerosols in version 1 of the US Department of Energy's Energy Exascale Earth System Model (E3SMv1). Our results show that HLD is responsible for 30.7 % of the total dust burden in the Arctic, whereas LLD from Asia and North Africa contributes 44.2 % and 24.2 %, respectively. Due to its limited vertical transport as a result of stable boundary layers, HLD contributes more in the lower troposphere, especially in boreal summer and autumn when the HLD emissions are stronger. LLD from North Africa and East Asia dominates the dust loading in the upper troposphere with peak contributions in boreal spring and winter. The modeled INP concentrations show better agreement with both ground and aircraft INP measurements in the Arctic when including HLD INPs. The HLD INPs are found to induce a net cooling effect (-0.24 W m -2 above 60 °N) on the Arctic surface downwelling radiative flux by changing the cloud phase of the Arctic mixed-phase clouds. The magnitude of this cooling is larger than that induced by North African and East Asian dust (0.08 and -0.06 W m -2 , respectively), mainly due to different seasonalities of HLD and LLD. Uncertainties of this study are discussed, which highlights the importance of further constraining the HLD emissions.

54 ENVIRONMENTAL SCIENCES↗

Responses of soil organic carbon to climate extremes under warming across global biomes

The impact of more extreme climate conditions under global warming on soil organic carbon (SOC) dynamics remains unquantified. Here, in this study, we estimate the response of SOC to climate extreme shifts under 1.5 °C warming by combining a space-for-time substitution approach and global SOC measurements (0–30 cm soil). Most extremes (22 out of 33 assessed extreme types) exacerbate SOC loss under warming globally, but their effects vary among ecosystems. Only decreasing duration of cold spells exerts consistent positive effects, and increasing extreme wet days exerts negative effects in all ecosystems. Temperate grasslands and croplands negatively respond to most extremes, while positive responses are dominant in temperate and boreal forests and deserts. In tundra, 21 extremes show neutral effects, but 11 extremes show negative effects with stronger magnitude than in other ecosystems. Our results reveal distinct, biome-specific effects of climate extremes on SOC dynamics, promoting more reliable SOC projection under climate change.

54 ENVIRONMENTAL SCIENCES↗

Impact of Changes to the Atmospheric Soluble Iron Deposition Flux on Ocean Biogeochemical Cycles in the Anthropocene

Abstract Iron can be a growth‐limiting nutrient for phytoplankton, modifying rates of net primary production, nitrogen fixation, and carbon export ‐ highlighting the importance of new iron inputs from the atmosphere. The bioavailable iron fraction depends on the emission source and the dissolution during transport. The impacts of anthropogenic combustion and land use change on emissions from industrial, domestic, shipping, desert, and wildfire sources suggest that Northern Hemisphere soluble iron deposition has likely been enhanced between 2% and 68% over the Industrial Era. If policy and climate follow the intermediate Representative Concentration Pathway 4.5 trajectory, then results suggest that Southern Ocean (>30°S) soluble iron deposition would be enhanced between 63% and 95% by 2100. Marine net primary productivity and carbon export within the open ocean are most sensitive to changes in soluble iron deposition in the Southern Hemisphere; this is predominantly driven by fire rather than dust iron sources. Changes in iron deposition cause large perturbations to the marine nitrogen cycle, up to 70% increase in denitrification and 15% increase in nitrogen fixation, but only modestly impacts the carbon cycle and atmospheric CO 2 concentrations (1–3 ppm). Regionally, primary productivity increases due to increased iron deposition are often compensated by offsetting decreases downstream corresponding to equivalent changes in the rate of phytoplankton macronutrient uptake, particularly in the equatorial Pacific. These effects are weaker in the Southern Ocean, suggesting that changes in iron deposition in this region dominates the global carbon cycle and climate response.

Hamilton, Douglas S.↗

Holocene paleoclimate change in the western US: The importance of chronology in discerning patterns and drivers

We report sediment in lakes and meadows forms a powerful archive that can be used to reconstruct environmental change through time. Reconstructions of lake level, of chemical, biological, and hydrological conditions, and of surrounding vegetation provide detailed information about past climate conditions, both locally and regionally. Indeed, most of our current knowledge of centennial- to millennial-scale climate variability in the arid western United States, where information about past hydroclimate is particularly important, comes from such sediment-based reconstructions. The pressing need for robust, precise predictions of future conditions is a significant motivation for paleoclimate science, and current research questions frequently require Holocene reconstructions to be resolved at sub-centennial timescales. Increasingly, regional syntheses seek to identify synoptic-scale patterns similar to those defined from modern observations (seasonal, interannual, multi-decadal, etc.) or to compare with the output of climate model simulations. However, the age control on existing records, especially those more than about 20 years old, is often sufficient only for millennial-scale interpretation. Here we assess the age control for 84 published and unpublished records from lakes and meadows in the Great Basin, California, and desert southwest, and use Bayesian modeling to evaluate the 95% uncertainty ranges for the 42 best-dated records. In the Late Holocene, about half of the 42 records have <400-year mean uncertainty ranges; however, high-precision age control is especially critical for young records, used to develop an accurate understanding of a proxy’s response to known climate variations. In the Middle Holocene, records vary from 400 to >800-year mean uncertainty and records of the Early Holocene have 600- to >1400-year mean uncertainty ranges. We find that the largest control on modeled uncertainties is dating density, with at least 2 dates/kyr being optimal and suggest obtaining “range-finder” dates at the onset of a study to better predict the total number of dates needed for an adequate age model. Such a density avoids a commonly observed phenomenon of significant peaks in uncertainty arising in gaps between age control points. Analysis of the uncertainties associated with proxy shifts reveal that more than half are >400 years. Although such large uncertainties currently prevent sub-centennial interpretations in most cases, increased dating density, strategic use of limited funds (including budgeting for a 2 date/kyr minimum at the proposal stage), construction of age-depth models with Bayesian methods, and critical evaluation of chronological uncertainty will shed light on past climate variability at finer timescales, enhancing our understanding of global and regional drivers of western U.S. climate.

54 ENVIRONMENTAL SCIENCES↗

What can the GRACE seasonal cycle tell us about lake-aquifer interactions?

Lake-aquifer interactions have been the subject of investigation and debate for decades. Traditional investigation methods include measurement of water flux across the groundwater-surface water interface, application of heat and environmental tracer methods, conducting numerical simulations of the water flow, and mass balance-based approaches. We first review, evaluate and describe the different approaches that have been applied for examining lake-aquifer interactions and propose an additional complementary approach. While the leakage from lakes and its slow movement through porous media (laminar flow) is well characterized by existing methods, modeling the rapid and turbulent flow through preferred pathways (faults/karst) remains a challenging task. To better understand the nature, and the full scale, of the lake aquifer interactions, and using Lake Nasser and the underlying Dakhla subbasin of the Nubian Sandstone Aquifer System (NSAS) in the Western Desert of Egypt (area: 0.66 x 10 6 km 2 ) as a test site, we examine, model, and correlate temporally and spatially, the variations in Gravity Recovery and Climate Experiment terrestrial water storage (GRACE TWS ) solutions, with precipitation, lake levels, area, and water volume. We review current GRACE applications in hydrology and present our novel approach. Findings include: firstly, large seasonal fluctuations (peak: Nov./Dec.; trough: July/Aug.) in Lake Nasser's surface water levels (average annual fluctuations: from 3.7 m to 7.5 m), area (average area: 3622 km 2 but up to 4530 km 2 ), and volume (annual average: 13.4 km 3 , and up to 34 km 3 ) are observed throughout years 2006 to 2015. These fluctuations are accompanied by an increase in GRACE TWS (average: 50 ± 13 mm/yr, up to 77 ± 18 mm/yr) over Lake Nasser and by a progression of a front of increasing GRACE TWS values (> 50 ± 13 mm) away from the lake reaching distances of up to 700 km some 3 to 5 months following peak lake level periods. The areas witnessing the seasonal increase in GRACE TWS display a progressive increase in phase and decrease in amplitude with distance from the lake. Secondly, the negligible precipitation over the Dakhla subbasin cannot account for the observed seasonal GRACE TWS patterns and neither can the leakage signal from Lake Nasser. Thirdly, overall similarities in the distribution of GRACE TWS seasonal spatial patterns are observed. During high lake level periods (e.g., Dec. 2007; Feb. 2015) additional, and more distant, areas from Lake Nasser saw increases in GRACE TWS and vice versa during low lake level periods (e.g., July 2006; Aug. 2010). These observations are consistent with Lake Nasser being the main source of modern recharge for the Dakhla subbasin and suggest a new conceptual model for the subbasin: a slow groundwater flow through a porous matrix and a rapid groundwater flow along a network of faults, fractures, and karst topography across the matrix. We suggest that the proposed conceptual model for the interaction between Lake Nasser and the Dakhla subbasin could be applicable to aquifers of similar geologic, climatic, and hydrologic settings worldwide and that approaches similar to those advocated here could be used to investigate the validity of this suggestion.

54 ENVIRONMENTAL SCIENCES↗

Area V: Waste Management and Subsurface Science (FY21-23 Final Report)

The objective of the Waste Management and Subsurface Science Topic Area V project is to evaluate the feasibility and safety of an intermediate-depth borehole for disposal of Israel Atomic Energy Commission (IAEC) nuclear waste. The IAEC nuclear waste is currently stored in above ground and/or in near-surface facilities that provide short-term radiological worker safety but do not provide long-term, full security and safeguards protection for the nuclear material. Disposal of the IAEC nuclear waste several hundred meters below the surface in a sealed borehole would provide long-term security and safeguards due to its depth underground, as well as long-term radiological safety, due to its isolation in a stable geological formation. This project supports the nuclear security and safeguards initiatives of the Science and Technology Working Group of the NNSA/IAEC Memorandum of Understanding (MOU) and addresses the Topic Area V objective and associated goals. The goals for Topic Area V are: (1) Assess the suitability of the vadose zone in the Yamin Plain of the northern Negev Desert, Israel as a host location for intermediate-depth borehole disposal of radioactive waste, (2) Define and refine the necessary characterization needs to better understand the regional climate, structure, and stratigraphy, geomechanics, geochemistry, hydrogeology, and seismicity of the stratigraphic sequence from the surface to the water table in the vicinity of the proposed host location for the disposal borehole(s), and (3) Develop a preliminary plan and schedule, including necessary resources and costs, for a borehole disposal concept and robust and transparent pre-closure and post-closure safety assessments. The safety assessments will require iteration with process level models at both site and regional scales. Upon completion, the information collected by this effort will allow the IAEC to make decisions regarding the viability and applicability of an intermediate depth (<500m) borehole for disposal in the vadose zone of the Yamin Plain. This FY21-23 final report is provided in lieu of a workshop report that was originally planned for delivery in January, 2024. Due to the conflict in Israel and associated delays, the workshop has been delayed to April 2024. The workshop will be held at LLNL but offsite and will switch to hybrid if travel restrictions remain in place. The associated workshop report will be provided as part of a new FY24-26 LCP.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

AmeriFlux CA-RSB Resolute Bay Polar Desert

This is the AmeriFlux version of the carbon flux data for the site CA-RSB Resolute Bay Polar Desert. Site Description - Polar desert on a gentle slope. Sparse grases and forbs in lower lying areas between microtopography in ice wedge polygon formations.

Arndt, Kyle [Woodwell Climate Research Center]↗

The tepary bean genome provides insight into evolution and domestication under heat stress

Tepary bean (Phaseolus acutifolis A. Gray), native to the Sonoran Desert, is highly adapted to heat and drought. It is a sister species of common bean (Phaseolus vulgaris L.), the most important legume protein source for direct human consumption, and whose production is threatened by climate change. Here, we report on the tepary genome including exploration of possible mechanisms for resilience to moderate heat stress and a reduced disease resistance gene repertoire, consistent with adaptation to arid and hot environments. Extensive collinearity and shared gene content among these Phaseolus species will facilitate engineering climate adaptation in common bean, a key food security crop, and accelerate tepary bean improvement.

54 ENVIRONMENTAL SCIENCES↗

Temperature, Humidity, and Time-Lapse Video Data from Yosemite National Park, Water Year 2024

This dataset contains time-lapse imagery and distributed measurements of air temperature, relative humidity, dew point, and soil temperature across Yosemite National Park from October 2023 to September 2024. Instruments were deployed at 10 sites in two cross-valley transects as part of the DOE Grant: Seasonal Cycles Unravel Mysteries of Missing Mountain Water organized by Jessica Lundquist (University of Washington), Rosemary Carroll (Desert Research Institute), and Ethan Gutmann (National Center for Atmospheric Research). The data are intended to support hydrologic modeling efforts to better resolve the fate of mountain water, and are published to support studies of surface climate or hydrologic processes in complex terrain. Measurements were collected with low-cost data loggers installed 2 m high on evergreen trees or buried just below the soil surface. A time-lapse camera at one site captures valley-scale seasonal snow cover variability.Dataset files are organized by site and variable (air measurements, ground measurements, or time-lapse video). Air and ground measurements are packaged in LoggerData.zip, and time-lapse imagery is compiled into a short video stored in TimelapseVideos.zip. File-level metadata contains details for each file included in the dataset. A data dictionary provides units and descriptions for column or row names in all files. The locations metadata file describes site characteristics, locations, and associated GPS methods.

54 ENVIRONMENTAL SCIENCES↗

Plant ecological genomics at the limits of life in the Atacama Desert

The Atacama Desert in Chile—hyperarid and with high–ultraviolet irradiance levels—is one of the harshest environments on Earth. Yet, dozens of species grow there, including Atacama-endemic plants. Herein, we establish the Talabre–Lejía transect (TLT) in the Atacama as an unparalleled natural laboratory to study plant adaptation to extreme environmental conditions. We characterized climate, soil, plant, and soil–microbe diversity at 22 sites (every 100 m of altitude) along the TLT over a 10-y period. We quantified drought, nutrient deficiencies, large diurnal temperature oscillations, and pH gradients that define three distinct vegetational belts along the altitudinal cline. Further, we deep-sequenced transcriptomes of 32 dominant plant species spanning the major plant clades, and assessed soil microbes by metabarcoding sequencing. The top-expressed genes in the 32 Atacama species are enriched in stress responses, metabolism, and energy production. Moreover, their root-associated soils are enriched in growth-promoting bacteria, including nitrogen fixers. To identify genes associated with plant adaptation to harsh environments, we compared 32 Atacama species with the 32 closest sequenced species, comprising 70 taxa and 1,686,950 proteins. To perform phylogenomic reconstruction, we concatenated 15,972 ortholog groups into a supermatrix of 8,599,764 amino acids. Using two codon-based methods, we identified 265 candidate positively selected genes (PSGs) in the Atacama plants, 64% of which are located in Pfam domains, supporting their functional relevance. For 59/184 PSGs with an Arabidopsis ortholog, we uncovered functional evidence linking them to plant resilience. As some Atacama plants are closely related to staple crops, these candidate PSGs are a “genetic goldmine” to engineer crop resilience to face climate change.

54 ENVIRONMENTAL SCIENCES↗

Urban agrivoltaics enhance crop resilience and food-energy synergies in a changing climate

Urban agrivoltaics, the synergistic integration of solar photovoltaics with urban agriculture, offers a transformative solution to food and energy insecurity, which are major barriers to sustainable urban development, especially in low-income urban areas facing intensified heat and water stress due to climate change. With 70% of the global population projected to live in cities by 2050, maximizing underutilized urban spaces is critical. Our study presents the first evaluation of ground-based agrivoltaics in an urban context, demonstrating that, while early-season yields may decline due to light reduction in temperate climates, productivity rebounds during periods of extreme heat, extending harvest windows and enhancing crop resilience. As cities seek climate-adaptive infrastructure, converting just a fraction of vacant land and rooftops to urban agrivoltaics can yield significant co-benefits such as generating renewable energy for thousands of households while supplying fresh produce to help alleviate food deserts.

14 SOLAR ENERGY↗

Atmospheric methane consumption in arid ecosystems acts as a reverse chimney and is accelerated by plant-methanotroph biomes

Drylands cover one-third of the Earth’s surface and are one of the largest terrestrial sinks for methane. Understanding the structure–function interplay between members of arid biomes can provide critical insights into mechanisms of resilience toward anthropogenic and climate-change-driven environmental stressors—water scarcity, heatwaves, and increased atmospheric greenhouse gases. This study integrates in situ measurements with culture-independent and enrichment-based investigations of methane-consuming microbiomes inhabiting soil in the Anza-Borrego Desert, a model arid ecosystem in Southern California, United States. The atmospheric methane consumption ranged between 2.26 and 12.73 μmol m 2 h −1 , peaking during the daytime at vegetated sites. Metagenomic studies revealed similar soil-microbiome compositions at vegetated and unvegetated sites, with Methylocaldum being the major methanotrophic clade. Eighty-four metagenome-assembled genomes were recovered, six represented by methanotrophic bacteria (three Methylocaldum , two Methylobacter , and uncultivated Methylococcaceae ). The prevalence of copper-containing methane monooxygenases in metagenomic datasets suggests a diverse potential for methane oxidation in canonical methanotrophs and uncultivated Gammaproteobacteria. Five pure cultures of methanotrophic bacteria were obtained, including four Methylocaldum . Genomic analysis of Methylocaldum isolates and metagenome-assembled genomes revealed the presence of multiple stand-alone methane monooxygenase subunit C paralogs, which may have functions beyond methane oxidation. Furthermore, these methanotrophs have genetic signatures typically linked to symbiotic interactions with plants, including tryptophan synthesis and indole-3-acetic acid production. Based on in situ fluxes and soil microbiome compositions, we propose the existence of arid-soil reverse chimneys, an empowered methane sink represented by yet-to-be-defined cooperation between desert vegetation and methane-consuming microbiomes.

59 BASIC BIOLOGICAL SCIENCES↗

Where does the dust deposited over the Sierra Nevada snow come from?

Abstract. Mineral dust contributes up to one-half of surface aerosol loading in spring over the southwestern United States, posing an environmental challenge that threatens human health and the ecosystem. Using self-organizing map (SOM) analysis with dust deposition and flux data from WRF-Chem and Modern-Era Retrospective analysis for Research and Applications, Version 2 (MERRA-2), we identify four typical dust transport patterns across the Sierra Nevada, associated with the mesoscale winds, Sierra barrier jet (SBJ), North Pacific High (NPH), and long-range cross-Pacific westerlies, respectively. We find that dust emitted from the Central Valley is persistently transported eastward, while dust from the Mojave Desert and Great Basin influences the Sierra Nevada during mesoscale transport occurring mostly in winter and early spring. Asian dust reaching the mountain range comes either from the west through straight isobars (cross-Pacific transport) or from the north in the presence of the NPH. Extensive dust depositions are found on the west slope of the mountain, contributed by Central Valley emissions and cross-Pacific remote transport. In particular, the SBJ-related transport produces deposition through landfalling atmospheric rivers, whose frequency might increase in a warming climate.

Huang, Huilin (ORCID:0000000273286738)↗

Genome resequencing reveals demographic history and genetic architecture of seed salinity tolerance in Populus euphratica

Populus euphratica is a dominant tree species in desert riparian forests and possesses extraordinary adaptation to salinity stress. Exploration of its genomic variation and molecular underpinning of salinity tolerance is important for elucidating population evolution and identifying stress-related genes. Here, we identify approximately 3.15 million single nucleotide polymorphisms using whole-genome resequencing. The natural populations of P. euphratica in northwest China are divided into four distinct clades that exhibit strong geographical distribution patterns. Pleistocene climatic fluctuations and tectonic deformation jointly shaped the extant genetic patterns. A seed germination rate-based salinity tolerance index was used to evaluate seed salinity tolerance of P. euphratica and a genome-wide association study was implemented. A total of 38 single nucleotide polymorphisms were associated with seed salinity tolerance and were located within or near 82 genes. Expression profiles showed that most of these genes were regulated under salt stress, revealing the genetic complexity of seed salinity tolerance. Furthermore, DEAD-box ATP-dependent RNA helicase 57 and one undescribed gene (CCG029559) were demonstrated to improve the seed salinity tolerance in transgenic Arabidopsis. These results provide new insights into the demographic history and genetic architecture of seed salinity tolerance in desert poplar.

59 BASIC BIOLOGICAL SCIENCES↗

Permafrost in the Cretaceous supergreenhouse

Earth’s climate during the last 4.6 billion years has changed repeatedly between cold (icehouse) and warm (greenhouse) conditions. The hottest conditions (supergreenhouse) are widely assumed to have lacked an active cryosphere. Here we show that during the archetypal supergreenhouse Cretaceous Earth, an active cryosphere with permafrost existed in Chinese plateau deserts (astrochonological age ca. 132.49–132.17 Ma), and that a modern analogue for these plateau cryospheric conditions is the aeolian–permafrost system we report from the Qiongkuai Lebashi Lake area, Xinjiang Uygur Autonomous Region, China. Significantly, Cretaceous plateau permafrost was coeval with largely marine cryospheric indicators in the Arctic and Australia, indicating a strong coupling of the ocean–atmosphere system. The Cretaceous permafrost contained a rich microbiome at subtropical palaeolatitude and 3–4 km palaeoaltitude, analogous to recent permafrost in the western Himalayas. A mindset of persistent ice-free greenhouse conditions during the Cretaceous has stifled consideration of permafrost thaw as a contributor of C and nutrients to the palaeo-oceans and palaeo-atmosphere.

54 ENVIRONMENTAL SCIENCES↗