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DOE OSTI2025

Visolis Microbial Chemical Intermediate Library Screening (CRADA Final Report)

Visolis is developing a commercial process for the bioproduction of a chemical intermediate, towards the derivative production of a portfolio of bio-based chemicals with large application potential, from drop-in commodity chemicals, to innovative specialty chemical offerings, to materials for multiple end markets and industries, which will ultimately benefit consumers and the public. Visolis already has several variants of a microbe that produce the chemical intermediate at distinct levels (i.e. high, medium, and low). They are currently pursuing transcriptomics analysis for some of these strains, towards a better understanding of the biology behind what makes certain strains perform better than others. There exist genome-scale library approaches for the microbe that could be used to screen gene disruption, overexpression, or repression candidates for perturbations to the production of the chemical intermediate. These approaches often reveal opportunities for further production improvement that are not otherwise accessible using hypothesis-driven metabolic engineering approaches. However, Visolis, while it could generate or procure such genome-scale libraries, does not have the automated strain engineering workflows required to screen thousands of variants, obtaining a production phenotype and a genotype for each. The purpose of this collaboration is to use LBNL and SNL capabilities to enable Visolis to effectively screen thousands of genome-scale library strain variants for phenotype/genotype relationships that will complement Visolis’ transcriptomic investigations.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

NASA NTRS1974

Time-fixed rendezvous by impulse factoring with an intermediate timing constraint

A method is presented for factoring a two-impulse orbital transfer into a three- or four-impulse transfer which solves the rendezvous problem and satisfies an intermediate timing constraint. Both the time of rendezvous and the intermediate time of a alinement are formulated as any element of a finite sequence of times. These times are integer multiples of a constant plus an additive constant. The rendezvous condition is an equality constraint, whereas the intermediate alinement is an inequality constraint. The two timing constraints are satisfied by factoring the impulses into collinear parts that vectorially sum to the original impulse and by varying the resultant period differences and the number of revolutions in each orbit. Five different types of solutions arise by considering factoring either or both of the two impulses into two or three parts with a limit for four total impulses. The impulse-factoring technique may be applied to any two-impulse transfer which has distinct orbital periods.

Green, R. N.↗

NASA NTRS1973

K-LL Auger transition probabilities for elements with low and intermediate atomic numbers

Radiationless K-LL transition probabilities have been calculated nonrelativistically in j-j coupling and in intermediate coupling, without and with configuration interaction, for elements with atomic numbers from 13 to 47. The system is treated as a coupled two-hole configuration. The single-particle radial wave functions required in the calculation of radial matrix elements, and in the calculation of mixing coefficients in the intermediate-coupling scheme, were obtained from Green's atomic independent-particle model. Comparison with previous theoretical work and with experimental data is made. The effects of intermediate coupling, configuration interaction, and relativity are noted.

Chen, M. H.↗

NASA NTRS1973

The identification of selected vegetation types in Arizona through the photointerpretation of intermediate scale aerial photography

The author has identified the following significant results. Nine photography interpretation tests were performed with a total of 19 different interpreters. Three tests were conducted with black and white intermediate scale photography and six tests with color infrared intermediate scale photography. The black and white test results show that the interpretation of vegetation mapped at the association level of classification is reliable for all the classes used at 61%. The color infrared tests indicate that the association level of mapping is unsatisfactory for vegetation interpretation of classes 1 and 6. Students' t-test indicated that intermediate scale black and white photography is significantly better than this particular color infrared photography for the interpretation of southeastern Arizona vegetation mapped at the association level.

Ross, G. F.↗

NASA NTRS1978

Intermediate-velocity gas in the local interstellar medium

Scans of interstellar ultraviolet absorption lines of N I, N II, and Si III for 17 stars are combined with previously published data for 30 stars. The extremal velocities at which detectable absorption occurs are tabulated, and it is shown that these are correlated for the three species. The data suggest that intermediate-velocity gas (20 to 60 km/s), best known from Na I and Ca II absorption, contains both neutral and ionized hydrogen. Features characteristic of intermediate-velocity isothermal shocks (greater than 60 km/s) are conspicuously rare. The intermediate-velocity gas may be in the form of clouds containing both H I and H II regions or of radiative shocks propagating in the interstellar medium; in the latter case the gas should be detectable in H-alpha emission.

Cowie, L. L.↗

NASA NTRS1980

Two-photon excitation cross-section in light and intermediate atoms

The method of explicit summation over the intermediate states is used along with LS coupling to derive an expression for two-photon absorption cross section in light and intermediate atoms in terms of integrals over radial wave functions. Two selection rules, one exact and one approximate, are also derived. In evaluating the radial integrals, for low-lying levels, the Hartree-Fock wave functions, and for high-lying levels, hydrogenic wave functions obtained by the quantum defect method are used. A relationship between the cross section and the oscillator strengths is derived. Cross sections due to selected transitions in nitrogen, oxygen, and chlorine are given. The expression for the cross section is useful in calculating the two-photon absorption in light and intermediate atoms.

Omidvar, K.↗

NASA NTRS1983

The intermediate wavelength magnetic anomaly field of the north Pacific and possible source distributions

A technique that eliminates external field sources and the effects of strike aliasing was used to extract from marine survey data the intermediate wavelength magnetic anomaly field for (B) in the North Pacific. A strong correlation exists between this field and the MAGSAT field although a directional sensitivity in the MAGSAT field can be detected. The intermediate wavelength field is correlated to tectonic features. Island arcs appear as positive anomalies of induced origin likely due to variations in crustal thickness. Seamount chains and oceanic plateaus also are manifested by strong anomalies. The primary contribution to many of these anomalies appears to be due to a remanent magnetization. The source parameters for the remainder of these features are presently unidentified ambiguous. Results indicate that the sea surface field is a valuable source of information for secular variation analysis and the resolution of intermediate wavelength source parameters.

Labrecque, J. L.↗

NASA NTRS1984

Acyl silicates and acyl aluminates as activated intermediates in peptide formation on clays

Glycine reacts with heating on dried clays and other minerals to give peptides in much better yield than in the absence of mineral. This reaction was proposed to occur by way of an activated intermediate such as an acyl silicate or acyl aluminate analogous to acyl phosphates involved in several biochemical reactions including peptide bond synthesis. The proposed mechanism has been confirmed by trapping the intermediate, as well as by direct spectroscopic observation of a related intermediate. The reaction of amino acids on periodically dried mineral surfaces represents a widespead, geologically realistic setting for prebiotic peptide formation via in situ activation.

White, D. H.↗

NASA NTRS1985

Intermediate-wavelength magnetic anomaly field of the North Pacific and posible source distributions

A technique that eliminates external field sources and the effects of strike aliasing was used to extract from marine survey data the intermediate wavelength magnetic anomaly field for (B) in the North Pacific. A strong correlation exists between this field and the Magsat field although a directional sensitivity in the Magsat field can be detected. The intermediate wavelength field is correlated to tectonic features. Island arcs appear as positive anomalies of induced origin likely due to variations in crustal thickness. Seamount chains and oceanic plateaus also are manifested by strong anomalies. The primary contribution to many of these anomalies appears to be due to a remanent magnetization. The source parameters for the remainder of these features are presently unidentified ambiguous. Results indicate that the sea surface field is a valuable source of information for secular variation analysis and the resolution of intermediate wavelength source parameters.

Labrecque, J. L.↗

NASA NTRS1985

Effect of reactive intermediates on the propagation of turbulent premixed flames

The most simple version of the Bray and Libby model of turbulent flame propagation has been extended to include a two-step reaction mechanism in which reactants decompose into reactive intermediates which then form products. A transport equation for the joint pdf of intermediate and product concentrations was derived, the pdf parameterized, and transport equations for the parameters obtained. The one-dimensional concentration equations were then solved for the parameters of the pdf and the turbulent kinetic energy. Flame speed and angle are recovered as eigenvalues of the problem. The results show the essential features observed in experiments. The product pdf is composed of peaks at zero and unity product concentration, with a continuous structure representing partially reacted gas. The intermediates pdf has a peak at zero concentration and a broad bell-shaped distribution at nonzero concentration, representing partially reacted gas. The results also show the effect of partitioning of energy release on the flame thickness and speed.

Azzazy, M.↗

NASA NTRS1990

Structure and evolution of small-amplitude intermediate shock waves

A simplified set of equations is derived that approximates the magnetohydrodynamic (MHD) Navier-Stokes equations for weakly nonlinear disturbances whose speeds are close to the MHD intermediate speed. Its shock structure solutions are then examined. The fast and slow shock solutions are uniquely specified by their Rankine-Hugoniot relations. However, the intermediate shock solutions, which are not unique, are characterized by the integral through the shock of the noncoplanar component of the magnetic field. For situations in which this integral is conserved, the Riemann problem is well defined and predicts the evolution of intermediate shocks. This analysis is substantiated by numerical computations.

Kennel, C. F.↗

NASA NTRS1992

Structure and evolution of time-dependent intermediate shocks

A quantitative description of time-dependent intermediate shocks is formulated using the Cohen-Kulsrud-Burgers equations. In noncoplanar Riemann problems, time-dependent two-three transition intermediate shocks evolve in time as a localized self-similar structure whose strength decreases as 1/the square root of t, and whose width expands as the square root of t. Time-dependent intermediate shocks offer a way of solving the noncoplanar MHD Riemann problem.

Wu, C. C.↗

NASA NTRS1992

The role of intermediate shocks in magnetic reconnection

The present study examines the structure of discontinuity layers associated with magnetic reconnection by numerically solving the Riemann problem for the evolution of an initial current sheet which separates two plasma regions with antiparallel magnetic field components in the z direction and a common guide magnetic field in the y direction. In the presence of a nonzero normal component of the magnetic field, the initial current sheet evolves into a system of MHD discontinuities. For the initial current sheet with a zero guide field, steady intermediate shocks, slow shocks, slow expansion waves, or contact discontinuity are observed to develop. For the current sheet with a nonzero guide field, time-dependent intermediate shocks, instead of steady intermediate shocks, are observed to bound the reconnection layer.

Lin, Y.↗

NASA NTRS1992

Structural relations for time-dependent intermediate shocks

A quantitative description of time-dependent (MHD) intermediate shocks is formulated. In non-coplanar Riemann problems, time-dependent 2 yields 3 intermediate shocks evolve in time as a localized self-similar structure whose strength decreases as 1/sq rt t, and whose width expands as sq rt t. We derive structural relations, similar to Rankine-Hugoniot relations, between the plasma properties and the magnitude of the transverse magnetic field, which we hope can help identify time-dependent 2 yields 3 intermediate shocks at the magnetopause, in the solar wind, and elsewhere in space. The analytic forms of the structural relations agree with our numerical results.

Wu, C. C.↗

NASA NTRS1993

Confirmation of solar cycle-dependent intermediate-degree p-mode frequency shifts

Results of intercomparisons of seven different sets of frequencies of intermediate-degree p-modes obtained at several different locations between 1981 and 1989 are presented. It is shown that the frequency shifts exhibited by all of these intermediate-degree p-modes are consistent with the intermediate-degree frequency shifts presented by Libbrecht and Woodward (1990) and also with the low-degree frequency shifts presented by Elsworth et al. (1990). It is also shown that these frequency shifts correlate with solar cycle-dependent changes in sunspot number, area, and irradiance. Unbinned and binned differences between 1984 Mount Wilson Observatory and revised 1981 South Pole frequencies are illustrated.

Rhodes, Edward J., Jr.↗

NASA NTRS1995

Kinetic structure of intermediate shocks: Implications for the magnetopause

A general study of the structure and stability of intermediate shocks (IS) in an isotropic plasma is presented using a hybrid as well as a resistive Hall MHD code. Special emphasis is put on the question of whether the rotational layers observed at the magnetopause can be intermediate shocks. The shocks are formed dynamically by the interaction between a flowing plasma and a stationary piston. Coplanar ISs (both strong and weak) are found to be stable in a collisionless plasma. The existence of slow shocks in a high beta plasma is also established for the first time. Noncoplanar ISs are found to be time-dependent, evolving toward a rotational discontinuity (RD) after some characteristic time tau which can be quite long (1000 Omega(exp -1), where Omega is the ion gyrofrequency). The value tau is larger the closer the rotation angle is to 180 deg. Rotations larger than 180 deg are found to be unstable, decaying into a state of minimum shear (i.e., rotation angle less than 180 deg). There are various length scales associated with an IS in the kinetic regime. The shortest scale is found to be the length scale over which rotation of the transverse component of the magnetic field takes place. This scale can have a half width as small as one ion inertial length (c/omega(sub p)) for electron sense rotations and 3c/omega(sub p) for ion sense rotations, for an upstream ion beta of unity. Both of these scales are consistent with the observed thickness at the magnetopause and identical to the corresponding RD scales. A detailed study of the mode conversion of the Alfven ion cyclotron waves (A/IC) waves across both slow and intermediate shocks and the resulting downstream wave spectrum are presented. The possibility that the large number of relfected ions observed at the magnetopause may be due to the presence of strong ISs is considered. The identification of strong ISs and their distinction from RDs should be possible in observations due to significant differences that exist between jump conditions and overall structure of the two discontinuities. The jumps in the plasma parameters across a weak IS are typically small. This together with the fact that the weak ISs and RDs have very similar thickness and other overall properties makes the distinction between weak ISs and RDs in the observations largely inconsequential. However, at large noncoplanarity angles the weak IS approaches the RD limit in a relatively short time (approximately less than 100 Omega (exp -1)). Thus, magnetopause rotations with large noncoplanarity angles are most likely either RDs or strong ISs. Finally, direct comparisons between fluid (resistive Hall MHD) and kinetic simulations show that fluid theory is not applicable to study of ISs in a collisionless plasma.

Karimabadi, H.↗

NASA NTRS1995

A method for shock determination and classification and Helios observations of fast, intermediate and slow interplanetary shocks

A new method to determine and classify shocks from in situ measurements is developed, using normalized velocities up- and down-stream in a velocity V(sub 1)-V(sub 2) diagram. With this method one can show how the shock solutions vary with different time averages over the data from the up- and down-stream region. For stable fast forward shocks the solutions are confined well in the 1 to 2 region, and for slow shocks most of the solutions are confined in the 3 to 4 region. A candidate for an intermediate shock was observed by Helios and with our method clearly identified. We found perhaps the first shock with parameters in the 2 to 3 region (with C(sub F1) greater than V(sub 1) greater than C(sub I1), and C(sub I2) greater than V(sub 2) greater than C(sub SL2) and a 180 deg rotation of the tangential magnetic field), which is interpreted as an intermediate shock with B(sub perpendicular 1) being less than B(sub perpendicular 2). The different shock solutions are somewhat distributed in the normalized V(sub 1)-V(sub 2) diagram, but only the intermediate shock solutions are consistent with the Rankine-Hugoniot relations for this particular shock. The Mach number M(sub I1) equals 1.067, a figure in good agreement with the Kennel et al. (1989) theoretical values.

Liu, S.↗

NASA NTRS2000

Observations of an Intermediate Layer During the Coqui II Campaign

NASA sounding rocket 21.114, launched March 7, 1998, during the Coqui II campaign, provided neutral wind and plasma density measurements of a weak intermediate layer. The layer was centered near 140 km and had an approximate peak plasma density of 2200 cc. The measured winds were typically less than 40 m/s, in agreement with wind shear formation theory and coincident density observations. The data obtained during the flight allow us to explore the plasma density structure and wind field morphology of the intermediate layer. Coupled with simultaneous data from Arecibo Observatory, the upleg and downleg density profiles provide three spatially separated measurements that enable the first detailed investigation of the horizontal extent and variation of an intermediate layer.

Bishop, R. L.↗