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At least 55 records · Page 3

Identifying Strain Stacking Boundaries between Multiphase Domains in Atomically Thin Two-Dimensional Magnets

Stacking engineering of van der Waals materials is an important strategy to control the materials’ properties, such as electronic correlations, ferroelectricity, and layer-dependent two-dimensional magnetism. A timely testbed for the study of the latter is atomically thin chromium trihalides (CrX 3 , X = Cl, Br, I). Notably, by understanding the sliding mechanism between different stacking sequences, control of the stacking arrangement, and thus magnetic properties in CrX 3 , can be achieved. Such insight, however, is currently lacking. Here, in this study, advanced electron microscopy methods are used to identify multiple stacking sequences corresponding to different bulk phases in atomically thin CrX 3 (X = Cl and Br) down to bilayer thickness and with lateral domain sizes as small as tens of nanometers. Indications of nanometer scale transitions and interactions at the stacking boundaries are found, including a universally preferred sliding direction that is consistent with density functional theory calculations and the strain fields at lateral heterostructure boundaries. This study demonstrates the necessity to consider local stacking structures when interpreting averaged magnetic properties measured with macroscale probes. Additionally, the preferred sliding direction insight from this study provides a strategy to control stacking sequence in atomically thin CrX 3 samples during the exfoliation and sample fabrication process.

DFT calculations

A parametric model study of the noise generated by the aerodynamic interaction of the tail rotor with the wake of the main rotor

Wind tunnel tests were conducted to determine the influence of various parameters on the noise produced by the interaction of the main rotor wake with the tail rotor. Parameters found to be of significance were the location of the main rotor vortex interaction on the tail rotor disk, the direction of rotation of the tail rotor, the lateral tail rotor-fin spacing, and the operating mode of the tail rotor. Of lesser importance were the main rotor thrust coefficient, longitudinal spacing between rotors, and tail rotor-to-main rotor rotational speed ratios. Analysis of the interaction noise characteristics using a simplified representation of the aerodynamic phenomena occurring during the interaction showed that existing predictive techniques could be modified and extended to predict the pertinent noise characteristics.

White, R. P., Jr.

Control of cavity noise

Experiments have been conducted on axisymmetric shallow cavity flows with the aim of investigating methods to modify cavity shear flows such that cavity noise may be reduced. The experiments indicate the presence of large gross lateral motion of the shear layer close to the downstream cavity corner which, on interaction with it, results in production of cavity flow noise. Results also show that the continuous injection of a fluid mass at the base of the cavity has a stabilizing effect on cavity shear flows. This stability is believed to have been achieved by supplying the mass required for cavity shear layer entrainment externally. This was also accompanied by a delay in large periodic lateral motion of the cavity shear layer close to the downstream cavity corner as observed without the mass injection. Thus the addition of a continuous fluid mass appears to be an effective means of suppressing cavity flow noise.

Sarohia, V.

Delta wing separation can dominate shuttle dynamics.

The unsteady aerodynamics of the delta wing, high cross range, shuttle orbiter have been investigated. Three potentially dangerous flow separation phenomena involving the delta wing have been identified, each of which could seriously compromise the flight dynamics. They are (1) leeside shock-induced separation, (2) sudden leading-edge stall, and (3) the subsonic leading-edge vortex and vortex burst. Each of these unsteady flow phenomena can be triggered by control surface deflection. Furthermore, wing stall and control-induced separation effects interact with the relatively large fuselage, causing simultaneous alteration of the lateral-directional stability. Trajectory shaping may be the most powerful means of dealing with these stall effects.

Reding, J. P.

Supersonic laminar base pressure, heat-transfer, and upstream influence correlations for small steps

Some results of the application of a unified theory of small disturbances in nonuniform high-speed boundary layers to the small-step problem for the case of supersonic laminar nonadiabatic flow are described. Comparisons with experimental data are given which show good agreement in a variety of physical features, including useful new base pressure and heat-transfer correlation relations. Using a small perturbation approach, an analytical theory is developed that predicts the steady-state pressure, temperature, and heat-transfer disturbance fields including suction or blowing through the step. Particular emphasis is placed on the important effects of the highly nonuniform flow across the boundary layer, lateral pressure gradients, and the upstream influence associated with viscous-inviscid interaction. Approximate analytical expressions for the wall pressure, skin friction, and heat-transfer perturbations and upstream influence distance are derived by a Fourier transformation approach.

Inger, G. R.

Space Shuttle Orbiter entry guidance and control system sensitivity analysis

An approach has been developed to determine the guidance and control system sensitivity to off-nominal aerodynamics for the Space Shuttle Orbiter during entry. This approach, which uses a nonlinear six-degree-of-freedom interactive, digital simulation, has been applied to both the longitudinal and lateral-directional axes for a portion of the orbiter entry. Boundary values for each of the aerodynamic parameters have been identified, the key parameters have been determined, and system modifications that will increase system tolerance to off-nominal aerodynamics have been recommended. The simulations were judged by specified criteria and the performance was evaluated by use of key dependent variables. The analysis is now being expanded to include the latest shuttle guidance and control systems throughout the entry speed range.

Stone, H. W.

Quorum sensing modulates microbial community structure through regulation of secondary metabolites

Bacteria are recognized for their diverse metabolic capabilities, yet the impact of microbe-microbe interactions on multispecies community structure and dynamics is poorly understood. Cell-to-cell signaling in the form of quorum sensing (QS) often regulates secondary metabolite production and microbial interactions. Here, we examine how acylhomoserine lactone (AHL)-mediated QS impacts microbial community structure in a 10-member synthetic community of isolates from Populus deltoides . To explore the role of QS in microbial community structure and dynamics, we disrupted AHL signaling by exogenous addition of AiiA-lactonase, an enzyme that cleaves the lactone ring. Microbial community structure resulting from signal inactivation, as measured by 16S rRNA amplicon sequencing and secondary metabolite production, was assessed after successive passaging of the community. Further, we investigated the impact of quorum quenching on specific microbe-microbe interactions using pairwise inhibition assays. Our results indicate that AHL inactivation alters the relative abundance of dominant community members at later passages but does not impact the overall membership in the community. Quorum quenching significantly alters the metabolic profile in lactonase-treated communities. This metabolic alteration impacts microbe-microbe interactions through decreased inhibition of other community members. Together, these results indicate that QS impacts microbial community structure through the regulation of secondary metabolites in dominant members and that the membership of microbial communities can be relatively stable despite changes in metabolic profiles.

59 BASIC BIOLOGICAL SCIENCES

EVT 16s Data and Large Supplementary Files

Soil microorganisms often interact to carry out decomposition of complex organic carbon and nitrogen compounds, such as chitin, but the high diversity and complexity of the soil microbiome and habitat has posed a challenge to elucidating such interactions between soil microorganisms. Here, we seek to address this challenge through analysis of a model soil consortium (MSC-2) of eight soil bacterial species. Our aim was to elucidate specific roles of the member species during chitin metabolism. Samples were collected from MSC-2 incubated in chitin-enriched soil over three months. Multi-omics was used to understand how the community composition, transcripts, proteins and chitin decomposition shifted over time. The data clearly and consistently revealed a temporal shift during chitin decomposition with defined contributions by individual species. A Streptomyces genus member (sp001905665) was a key player in early steps of chitin decomposition, with other MSC-2 members being central in carrying out later steps. These results illustrate how multi-omics applied to a defined consortium untangles interactions between soil microorganisms.

McClure, Ryan [Pacific Northwest National Laborato

Multi-omics of a model bacterial consortium deciphers details of chitin decomposition in soil

Soil microorganisms interact to carry out decomposition of complex organic carbon and nitrogen compounds, such as chitin, but the high diversity and complexity of the soil microbiome and habitat have posed a challenge to elucidating such interactions. Here, we sought to address this challenge by analysis of a model soil consortium (MSC-2) consisting of eight soil bacterial species. Our aim was to elucidate the specific roles of the member species during chitin metabolism. Samples were collected from MSC-2 incubated in chitin-enriched soil over 3 months. Multi-omics was used to understand how the community composition, transcripts, proteins, and chitin decomposition shifted over time. The data clearly and consistently revealed a temporal shift during chitin decomposition with defined contributions by individual species. A Streptomyces genus member (sp001905665) was a key player in early steps of chitin decomposition, with other MSC-2 members being central in carrying out later steps. These results illustrate how multi-omics applied to a defined consortium untangles the interactions between soil microorganisms.

chitin

Knowledge graph-aided Bayesian active learning for top- K genetic interaction discovery

In silico methods for predicting the effects of multi-gene perturbations hold great promise for advancing functional genomics, computational drug discovery, and disease modeling. However, the development of these predictive algorithms for mammalian systems has been hampered by limited datasets and high experimental costs. In this study, we present a Bayesian active learning framework designed to discover pairwise host gene knockdowns that effectively inhibit viral proliferation in an in vitro HIV-1 infection model. Our method leverages a biological knowledge graph as side information and employs a computationally efficient batch diversification approach. We evaluated this framework using a dataset of viral load measurements obtained from multi-day dual-gene depletion experiments, encompassing all possible pairwise knockdowns of over 350 host genes associated with HIV infection. We demonstrate that our framework rapidly identifies the most effective gene knockdown pairs for reducing viral load. Furthermore, we show that incorporating side information enhances performance during the early stages of active learning (low data regime), while our batch diversification strategy significantly boosts performance in later stages (high data regime). This framework is general and can be adapted to explore gene interactions in other contexts, such as synthetic lethality prediction and mapping epistatic effects across quantitative trait loci.

Computational biology and bioinformatics

A Novel Measurement of the Anomalous Muon Spin Precession Frequency in the Muon $g-2$ Experiment at Fermilab

The Muon $g-2$ Experiment operated at Fermi National Accelerator Laboratory (FNAL, or Fermilab) between 2018 and 2023 to produce the world's most precise measurement of the muon's \textit{anomalous magnetic moment}, $a_\mu = \frac{g_\mu - 2}{2}$, which expresses the relative deviation in the muon's $g$-factor from a baseline theoretical expectation that $g_\mu = 2$. In the Standard Model of particle physics, $g_\mu > 2$ and hence $a_\mu > 0$ by a calculable amount that depends on all possible interactions between the muon and all other fundamental particles, including any potentially undiscovered interactions beyond the Standard Model. For this reason, measurements of the electron anomaly $a_e$ and later the muon anomaly $a_\mu$ have helped guide the development of the Standard Model since the inception of quantum field theory, and the measured value of $a_\mu$ provides a valuable constraint for new hypotheses that extend the Standard Model. As of 2006, the leading measurement and Standard Model prediction for $a_\mu$ exhibited tension at the level of about three standard deviations, motivating an improved measurement at Fermilab that could test the tension more precisely. The experiment functions by storing a polarized beam of $\mu^+$ in a uniform magnetic field, which simultaneously induces circular motion and spin precession. As the stored muons undergo the Michel decay $\mu^+ \to e^+ + \nu_e + \bar{\nu}_\mu$, mediated by the parity-violating weak interaction, the rest-frame $e^+$ emission direction is correlated with the parent $\mu^+$ spin orientation. Boosting into the laboratory frame encodes this correlation in the decay $e^+$ energy, which is higher when the emission (i.e. $\mu^+$ spin direction) is aligned with the $\mu^+$ momentum, and lower when opposite. Detectors then count the rate of high-energy decay $e^+$, which modulates at the difference between the $\mu^+$ revolution and spin precession frequencies. This observed frequency, called the \textit{anomalous spin precession frequency} $\omega_a$, is directly proportional to $a_\mu$. The extraction of $\omega_a$ proceeds by fitting the time spectrum of detected $e^+$, which requires precise modeling of the $\omega_a$ oscillation as well as any perturbations from beam dynamics and detector acceptance. Using the $\omega_a$ analysis presented in this work, based on Runs 4 -- 6 of the Muon $g-2$ Experiment at Fermilab, we find that $a_\mu = 0.001\,165\,920\,738(162)$ with a relative uncertainty of 139 parts per billion.

Barrett, Tyler [Cornell U.]

Plant-microbial interplay for organic nitrogen mediated by functional specificity of root compartments

The organic form of nitrogen (N) is a critical intermediate in mutualistic and competitive root-microbial interactions, mediated by extracellular enzymes. Visualization of the hotspots of organic N and proteolytic activity might be valuable for revealing root functional specificity in N acquisition and transformation at the level of individual roots and compartments. For the first time, we used time-lapse amino-mapping and zymography to co-localize and map the spatial distribution of amino-N and leucine aminopeptidase (LAP) activity in the soil and different root parts of maize (Zea mays L.). Amino-N distribution was mainly associated with seminal roots and root tips, where it overlapped with LAP activity hotspots. In the lateral roots and bulk soil, however, LAP activity was decoupled from amino-N. Distinct functional traits revealed themselves as the highest amino-N content and LAP activity in seminal root tips and as the largest relative extent of the rhizosphere in lateral root tips. Co-localized amino-N and LAP activities highlighted different nutrient acquisition strategies mediated by root-microbe interactions, depending on the root compartment. Seminal roots and their tips appeared to adopt mutualistic strategies, potentially attracting root-associated microorganisms through releasing oligo- and polypeptides. In contrast, lateral roots, with amino-N detected only at their tips, demonstrated stronger N competition, relying on the enzyme activity of the rhizosphere microbial community for N acquisition. These insights emphasized the role of root functional specialization in shaping plant-microbe interactions, offering pathways to enhance nutrient use efficiency.

Maize (Zea mays L.)

Wind tunnel and flight development of spoilers for general aviation aircraft

Wind tunnel tests have been carried out to develop a spoiler lateral control system for use with the GA(W)-1 airfoil with a 30% Fowler flap. Tests show that unfavorable aerodynamic interactions can occur between spoiler and flap for large flap deflections. Providing venting of lower surface air through the spoiler opening substantially improves performance. Results of tests with a number of spoiler and cavity shapes are presented and discussed. Applications of two-dimensional wind tunnel results to the design of satisfactory manual lateral control systems are discussed.

Wentz, W. H., Jr.

Connectivity, Pathology, and ApoE4 Interactions Predict Longitudinal Tau Spatial Progression and Memory

ABSTRACT Tau pathology spread into neocortex indicates a transition from healthy aging to Alzheimer's disease (AD). Connectivity between tau epicenters and later accumulating regions of cortex has been proposed as a mechanism of tau spread, but how this relationship changes with greater AD pathology burden or genotype is not understood. We investigated tau accumulation in two key regions, precuneus and inferior temporal cortex, using resting state functional connectivity (rsFC) and longitudinal PET imaging from a multicohort sample of cognitively unimpaired older adults. We examined how baseline tau PET, Aβ PET, and ApoE4 genotype status interact with rsFC between hippocampus and these downstream regions to predict rate of tau accumulation in neocortex. We found that the 3‐way interaction between connectivity, baseline tau, and baseline Aβ or ApoE4 status was associated with neocortical tau accumulation in precuneus and inferior temporal cortex. In addition, baseline tau, Aβ, and ApoE4 status also moderated the association between connectivity and rate of memory decline. Together, these results suggest that the extent and distribution of future tau accumulation may be predicted by the interaction of baseline connectivity, AD pathology, and genetic risk.

Neurosciences & Neurology

Otolithic influences on extraocular and intraocular muscles

Selective stimulation of utricular gravireceptors leads to gross activation of the bulbar reticular formation where a strong interaction with evoked spino-bulbo-spinal reflex activity occurs. The utricular neurons encountered by microelectrodes in the lateral vestibular nuclei show four types of elicited activity; two of these display an increased firing rate, and two exhibit pronounced inhibitory effects. Application of a stimulus of long duration and constant intensity to the utricle has shown that rapid adaptation of the peripheral receptors is a prominent feature. The effects of selective utricular stimulation upon eye movements, as recorded by the corneoretinal potential method, have been studied in experiments on cats and monkeys and it can be firmly stated that prolonged stimulation of the utricle can evoke strong primary nystagmus, followed by a secondary nystagmus at the cessation of stimulation. The action of utricular stimulation on ocular reflexes has been examined further, with particular attention to evoked pupillary reactions in both cats and monkeys: constriction during the fast phase of the brisk conjugate eye movement, and dilatation during the flow phase.

Gernandt, B. E.

Amapari Marker Band Metal‐Enrichments: Potential Mechanisms and Implications for Surface and Subsurface Water and Weathering in Gale Crater

NASA's Curiosity rover is exploring a 5 km tall sedimentary mound that is hypothesized to record the transition from a warm and wet (phyllosilicate-rich) to a cold and drier (sulfate-rich) Mars. Evidence of magnesium sulfate-bearing rock has shown that Curiosity has crossed through this phyllosilicate-sulfate transition. Recently, Curiosity arrived at the Amapari Marker Band, a darker, indurated unit that can be traced laterally for tens of kilometers in orbiter images. Here, Curiosity found evidence for a very broad lake, and bedforms interpreted as wave-ripple laminated sedimentary rock that likely was deposited in shallow water in the explored location, before becoming a deeper lake. These rocks are enriched in Fe, Mn, and Zn which has major implications for groundwater paleohydrology in Gale crater. Three formation hypotheses are considered: concretion formation during early diagenetic alteration of shallow lake sediments, laterization or leaching of the sediments, and addition of Fe, Mn, and Zn by a mildly acidic and reducing groundwater interacting with a redox and/or pH front in a stratified lake. The preferred interpretation of the metal enrichments within the Amapari Marker band sedimentary rocks is that they formed in a shallow water environment at a redox and/or pH front within the ripple unit, which drove precipitation and concentration of metals. If the enrichments are due to groundwater alteration, these processes could link subsurface and surface environments. Water and the presence of high amounts of redox sensitive elements and other metals are favorable indicators for habitability.

58 GEOSCIENCES

Mineral-associated organic matter is heterogeneous and structured by hydrophobic, charged, and polar interactions

The formation of mineral-associated organic matter (MAOM) is a key phenomenon that may explain the slow turnover rates of carbon in soil organic matter (SOM). Despite this, important details pertaining to the structure and dynamics of MAOM remain unknown. In the present study, we use replica-exchange molecular dynamics simulations to gain insight into the structure of MAOM on the surface of prototypical phyllosilicate clay and Fe-oxide minerals, montmorillonite and goethite, fine-grained minerals that strongly impact soil carbon dynamics in temperate and tropical regions, respectively. We examine the impact of aqueous chemistry through the presence of either Na + or Ca 2 + charge balancing counterions. Our results are consistent with the hypothesized multilayer sorption (“onion-skin”) model of MAOM and help to explain previous observations regarding the patchy distribution of SOM on mineral surfaces. In particular, the SOM coatings are partial and laterally heterogeneous, and water retains extensive access to mineral surfaces even when significant SOM sorption occurs. Low molecular weight neutral SOM molecules ( < 200 Da) infrequently interact with the mineral surfaces nor their sorbed organic matter coatings and are increasingly labile with decreasing molecular weight. This observation is inconsistent with a central feature of the predominant soil continuum model of SOM and suggests that further iterations of the conceptual model may be required.

54 ENVIRONMENTAL SCIENCES

Hydrology controls thermokarst and alters carbon cycling and methane emissions in peatlands near the southern limit of permafrost

Permafrost peatlands store vast amounts of frozen carbon across northern landscapes. When ground ice melts, surface subsidence produces thermokarst landforms that expand wetlands at the edges of permafrost plateaus. Thermokarst represents an accelerating climate feedback, but uncertainties remain about how ground ice, hydrology, and vegetation interact to shape landscape change and carbon fluxes. We extended the process-based model ecosys to simulate thermokarst dynamics in laterally coupled 2D transects at a well-characterized boreal peatland site in Canada’s Northwest Territories. After benchmarking against site observations, we varied ground ice content and hydrologic boundary conditions across ranges typical near the southern permafrost limit. Simulations revealed distinct degradation regimes governed by the elevation difference between the frost table and the external water table. Rates of lateral retreat, the thaw-driven encroachment of wetlands into adjacent plateaus, ranged from 0 to >2 m yr −1 under identical weather forcing, consistent with observations and highlighting the strong role of WT and ground ice. Simulated vegetation dynamics indicate that black spruce mortality cannot be explained by anoxia alone, pointing to additional stressors such as root damage, pathogens, or physical destabilization. Despite large hydrologic shifts, net ecosystem CO 2 exchange remained a slight sink after collapse, while methane (CH 4 ) emissions rose by one to two orders of magnitude. As a result, lateral retreat substantially increases the greenhouse warming potential of permafrost peatlands (1.7 million km 2 in area), with simulated emissions of 0.1–10 Mt CO 2 -eq decade −1 depending on hydrology and retreat rates. These results underscore the need to account for both ground ice and hydrologic dynamics when assessing thermokarst-driven climate feedbacks.

carbon cycling