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NASA NTRS2004

Life Detection on the Early Earth

Finding evidence for first the existence, and then the nature of life on the early Earth or early Mars requires both the recognition of subtle biosignatures and the elimination of false positives. The history of the search for fossils in increasingly older Precambrian strata illustrates these difficulties very clearly, and new observational and theoretical approaches are both needed and being developed. At the microscopic level of investigation, three-dimensional morphological characterization coupled with in situ chemical (isotopic, elemental, structural) analysis is the desirable first step. Geological context is paramount, as has been demonstrated by the controversies over AH84001, the Greenland graphites, and the Apex chert microfossils . At larger scales, the nature of sedimentary bedforms and the structures they display becomes crucial, and here the methods of condensed matter physics prove most useful in discriminating between biological and non-biological constructions. Ultimately, a combination of geochemical, morphological, and contextural evidence may be required for certain life detection on the early Earth or elsewhere.

Runnegar, B.↗

NASA NTRS1987

Determine precipitation rates from visible and infrared satellite images of clouds by pattern recognition technique

A more advanced cloud pattern analysis algorithm was subsequently developed to take the shape and brightness of the various clouds into account in a manner that is more consistent with the human analyst's perception of GOES cloud imagery. The results of that classification scheme were compared with precipitation probabilities observed from ships of opportunity off the U.S. east coast to derive empirical regressions between cloud types and precipitation probability. The cloud morphology was then quantitatively and objectively used to map precipitation probabilities during two winter months during which severe cold air outbreaks were observed over the northwest Atlantic. Precipitation probabilities associated with various cloud types are summarized. Maps of precipitation probability derived from the cloud morphology analysis program for two months and the precipitation probability derived from thirty years of ship observation were observed.

Weinman, James A.↗

NASA NTRS1987

Circumnuclear starbursts in Seyfert galaxies

Observational diagnostics for the recognition of circumnuclear star formation in Seyfert galaxies are described and illustrated. These methods include: (1) spatially resolved optical spectroscopy, which allows the emission lines for HII regions to be separated from those originating in gas ionized by the Seyfert nucleus; (2) radio continuum mapping, where the linear radio sources characteristic of the nuclear activity may be distinguished from the diffuse morphology of multiple supernova remnants generated in a starburst; (3) infrared spectroscopic searches for emission features of dust, which are seen in starbursts but not in Seyfert nuclei; (4) the shape of the IRAS spectrum. These various diagnostics agree well as to the presence or absence of ongoing star formation. The IRAS spectra of a significant fraction of Seyferts are dominated by emission from dust heated by stars, not the Seyfert nucleus itself. In these cases, the spectrum is curved, being steep between 25 and 60 microns and flatter between 60 and 100 microns. When the Seyfert nucleus dominates, the 25 to 100 micron spectrum is much flatter. It is suggested that the location of a Seyfert galaxy in the IRAS color-color diagram reflects primarily the relative contributions of the active nucleus and dust heated by stars to the infrared fluxes.

Wilson, Andrew S.↗

NASA NTRSDate not supplied

TPSAS-NF1676L-21193-DND

Researchers at NASA Langley Research Center have been developing an automated pattern recognition algorithm to identify overshooting convective cloud tops (OTs) in support of the GOES-R satellite program. This algorithm identify regions of overshooting at the individual 1-4 km geostationary satellite pixel scale using visible (during daytime only) and infrared channel imagery and numerical weather analysis data. The algorithm has been developed based upon analysis of 0.25-1 km spatial resolution Aqua MODIS imagery, using a database of over 2000 manually identified OT features throughout the world in storms with varying intensity and morphology. The OT database includes storms ranging from small, warm topped cells in Alaska and Mongolia, tornadic supercells over the U.S. Central Plains and Europe, large tropical mesoscale convective systems, and overshooting in the eyewalls and spiral bands of category 5 tropical cyclones. This database is available for use by the research community. The algorithm is designed to operate on data from any current and historical satellite imager, allowing for development of a highly accurate global OT detection climatology that extends back into the 1990's at up to a 15-30 min temporal resolution throughout the diurnal cycle. As members of the McIDAS Users Group, NASA LaRC has immediate access to the full global archive of geostationary imager data which would allows rapid development of OT climatologies and short-term databases. This type of capability has never been available within the weather and climate research community. Regional geostationary OT databases have been already developed over CONUS during SEAC4RS, for 18-years over the Eastern U.S., and for 5-10 years over Australia, Europe, Southeast Asia, and East Africa among many other regions. Some of these datasets are being used by climate researchers and private industry to examine UTLS-penetrating storm spatial distributions and their temporal variability, in addition to weather hazards associated with these storms at unprecedented spatial detail. This presentation will describe the OT pattern recognition algorithm and highlight recent product applications.

Kristopher Bedka↗

DOE OSTI2026

Melatonin-Induced Modulation of Cholesterol-Enriched Model Neuronal Membranes

Melatonin, a hormone primarily produced by the brain’s pineal gland, not only regulates circadian rhythms, but also influences the structural and biophysical properties of neuronal membranes. Its amphiphilic nature enables direct incorporation into lipid bilayers and preferential interactions with cholesterol-rich lipid rafts, critical hubs for cellular signaling and membrane organization. Despite increasing recognition of its membrane activity, the molecular basis of melatonin’s interactions with coexisting liquid-ordered (L o ) and liquid-disordered (L d ) phases remains unclear. Here, in this study, we combine small-angle neutron scattering (SANS) and all-atom molecular dynamics simulations to examine model neuronal membranes composed of DSPC, DOPC, POPC, and cholesterol. Our results show that melatonin preserves domain morphology while adopting distinct orientations within the bilayer and at the membrane interface, allowing both lateral and transmembrane bridging across lipid phases. These findings establish the molecular underpinnings of melatonin’s modulation of membrane heterogeneity and provide strong support for its receptor-independent actions.

atomistic simulations↗

NASA NTRS1975

Experimentally reproduced textures and mineral chemistries of high-titanium mare basalts

Many of the textures, morphologies, and mineral chemistries of the high-titanium mare basalts have been experimentally duplicated using single-stage cooling histories. Lunar high-titanium mare basalts are modeled in a 1 m thick gravitationally differentiating flow based on cooling rates, thermal models, and modal olivine contents. The low-pressure equilibrium phase relations of a synthetic high-titanium basalt composition were investigated as a function of oxygen fugacity, and petrographic criteria are developed for the recognition of phenocrysts which were present in the liquid at the time of eruption.

Usselman, T. M.↗

DOE OSTI2020

Determination of the Maturation Status of Dendritic Cells by Applying Pattern Recognition to High-Resolution Images

The maturation or activation status of dendritic cells (DCs) directly correlates with their behavior and immunofunction. A common means to determine the maturity of dendritic cells is from high-resolution images acquired via scanning electron microscopy (SEM) or atomic force microscopy (AFM). While direct and visual, the determination has been made by directly looking at the images by researchers. Here we report a machine learning approach using pattern recognition in conjunction with cellular biophysical knowledge of dendritic cells to determine the maturation status of dendritic cells automatically. The determination from AFM images reaches 100% accuracy. The results from SEM images reaches 94.9%. The results demonstrate the accuracy of using machine learning for accelerating data analysis, extracting information, and drawing conclusions from high-resolution cellular images, paving the way for future applications requiring high-throughput and automation, such as cellular sorting and selection based on morphology, quantification of cellular structure, and DC-based immunotherapy.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

NASA NTRS2020

Pattern Identification - A Foundation for Research in the Emphasis of Design Patterns in Systems Engineering and Knowledge Capture

Pattern Language describes the morphology and functionality of a system in the absence of design particulars. Harnessing this capability will provide the Systems Engineering discipline a means of managing the development of increasingly complex systems with increasingly distributed design teams while capturing and retaining knowledge for future generations. Pattern Language is a syntax for describing, and structurally relating, design patterns. Design patterns contextually describe the application of domain knowledge in the engineered solution to the force balance problem. The parallels between pattern recognition and application, as a fundamental stage of human learning, and pattern observation within a complex system, suggests pattern language may be a valuable tool in the capture and dissemination of knowledge. Pattern application has enjoyed considerable study over the last several decades, however much of this work has focused on the replication of design particulars. This work returns to the roots of Pattern Language and explores the utility of patterns as an architectural description and guide, and knowledge capture method, for complex system development beginning with the identification of a time proven design pattern.

Russell, Samuel P.↗

NASA NTRS1993

Classification of Martian deltas

Water-borne sediments in streams are deposited, upon eventual cessation of flow, either as deltas or as alluvial fans or plains. Deltas and alluvial fans share a common characteristic; both may be described as deposition Al plains at the mouth of a river or stream. A delta is formed where a stream or river deposits its sedimentary load into a standing body of water such as an ocean or lake. An alluvial fan is produced where a stream loses capacity by a greatly decreased gradient. A delta has subaerial and subaqueous components, but an alluvial fan is entirely subaerial. In terrestrial conditions, deltas and alluvial fans are reasonably distinct landforms. The juxtaposition of concomitant features composition and internal structure are sufficiently explicit as to avoid any confusion regarding their proper identification on Mars, the recognition of deltas and their distinction from alluvial fans is made difficult by low resolution imaging. Further, although it may be demonstrated that standing bodies of water existed on the surface of Mars, many of these bodies may have existed for extremely short periods of time (a few days to months); hence, distinctive shoreline features were not developed. Thus, in an attempt to derive a Martian classification of deltas, the inclusion of wholly subaerial deposits may be unavoidable. A simple, broad, morphological classification of Martian deltas, primarily on planimetric shape, includes digitate deltas, fan-shaped deltas, and re-entrant deltas. A fourth, somewhat problematical class includes featureless plains at the end of many valley systems.

Dehon, R. A.↗

NASA NTRS1995

Geophysical phenomena classification by artificial neural networks

Space science information systems involve accessing vast data bases. There is a need for an automatic process by which properties of the whole data set can be assimilated and presented to the user. Where data are in the form of spectrograms, phenomena can be detected by pattern recognition techniques. Presented are the first results obtained by applying unsupervised Artificial Neural Networks (ANN's) to the classification of magnetospheric wave spectra. The networks used here were a simple unsupervised Hamming network run on a PC and a more sophisticated CALM network run on a Sparc workstation. The ANN's were compared in their geophysical data recognition performance. CALM networks offer such qualities as fast learning, superiority in generalizing, the ability to continuously adapt to changes in the pattern set, and the possibility to modularize the network to allow the inter-relation between phenomena and data sets. This work is the first step toward an information system interface being developed at Sussex, the Whole Information System Expert (WISE). Phenomena in the data are automatically identified and provided to the user in the form of a data occurrence morphology, the Whole Information System Data Occurrence Morphology (WISDOM), along with relationships to other parameters and phenomena.

Gough, M. P.↗

NASA NTRS2004

Lunar and Planetary Science XXXV: Mars

The session "Mars" included the following reports:Tentative Theories for the Long-Term Geological and Hydrological Evolution of Mars; Stratigraphy of Special Layers Transient Ones on Permeable Ones: Examples from Earth and Mars; Spatial Analysis of Rootless Cone Groups on Iceland and Mars; Summer Season Variability of the North Residual Cap of Mars from MGS-TES; Spectral and Geochemical Characteristics of Lake Superior Type Banded Iron Formation: Analog to the Martian Hematite Outcrops; Martian Wave Structures and Their Relation to Mars; Shape, Highland-Lowland Chemical Dichotomy and Undulating Atmosphere Causing Serious Problems to Landing Spacecrafts; Shear Deformation in the Graben Systems of Sirenum Fosssae, Mars: Preliminary Results; Components of Martian Dust Finding on Terrestrial Sedimentary Deposits with Use of Infrared Spectra; Morphologic and Morphometric Analyses of Fluvial Systems in the Southern Highlands of Mars; Light Pattern and Intensity Analysis of Gray Spots Surrounding Polar Dunes on Mars; The Volume of Possible Ancient Oceanic Basins in the Northern Plains of Mars MARSES: Possibilities of Long-Term Monitoring Spatial and Temporal Variations and Changes of Subsurface Geoelectrical Section on the Base; Results of the Geophysical Survey Salt/Water Interface and Groundwater Mapping on the Marina Di Ragusa, Sicily and Shalter Island, USA ;A Miniature UV-VIS Spectrometer for the Surface of Mars; Automatic Recognition of Aeolian Ripples on Mars; Absolute Dune Ages and Implications for the Time of Formation of Gullies in Nirgal Vallis, Mars; Diurnal Dust Devil Behaviour for the Viking 1 Landing Site: Sols 1 to 30; Topography Based Surface Age Computations for Mars: A Step Toward the Formal Proof of Martian Ocean Recession, Timing and Probability; Gravitational Effects of Flooding and Filling of Impact Basins on Mars; Viking 2 Landing Site in MGS/MOC Images South Polar Residual Cap of Mars: Features, Stratigraphy, and Changes.

Source record↗

NASA NTRS1974

Identification characteristics of certain lunar relief forms on pictures obtained from spacecraft

This investigation was restricted to the determination of recognitional characteristics of a single type of relief form, dimple craters on the surface of maria. These craters were arbitrarily considered as closed, negative relief forms, circular in plan-view, and symmetric relative to the center. Two techniques were used to determine the shadow configuration in the craters: (1) experimental determination of the shadow edge by illuminating the models of different profile shapes with a parallel light beam; and (2) geometric construction of the shadow edge using a vertical section of the formation in the direction of the solar rays. A morphological map of the surface which includes the following factors can be compiled using the proposed identification technique: crater profile nature, crater diameter, ratio of diameter and depth, sharpness of the lip, and distribution density of the forms.

Kravtsova, V. I.↗

DOE OSTI2021

Scavenger receptor MARCO contributes to macrophage phagocytosis and clearance of tumor cells

Highlights: • The MARCO receptor on macrophages was positively correlated with the ability to phagocyte tumor cells. • Macrophage MARCO affects cytoskeleton rearrangement and pseudopodia formation through the SYK-PI3K-Rac1 pathway. • Integrin is the ligand of MARCO on the tumor cells. Macrophage receptor with collagenous structure (MARCO) is a member of the class A scavenger receptor family which is expressed on the cell surface of macrophages. It is well known that MARCO avidly binds to unopsonized pathogens, leading to its ingestion by macrophages. However, the role of MARCO in the recognition and phagocytosis of tumor cells by macrophages remains poorly understood. In this study, we used lentiviral technology to knockdown and overexpress MARCO and investigated the ability of macrophages to phagocytose tumor cells. Our results showed that MARCO expression was correlated with the ability of macrophages to carry on phagocytosis. MARCO knockdown led to significant decreases in the number of engulfment pseudopodia of macrophages and inhibition of the phagocytosis of tumor cells. On the other hand, MARCO overexpression elevated activity of SYK, PI3K and Rac1 in macrophages, which led to changes in macrophage morphology and enhanced phagocytosis by promoting formation of stress fibers and pseudopodia. By Co-IP analysis we showed that MARCO directly binds to the β5 integrin of SL4 tumor cells. In summary, these results demonstrated the important role for MARCO in demonstrated tumor cells uptake and clearance by macrophages.

60 APPLIED LIFE SCIENCES↗

NASA NTRS1972

Remote sensing of changes in morphology and physiology of trees under stress

Previsual detection of Fomes annosus in pine plantations was studied. Detailed analyses of photographic imagery obtained over the Ann Arbor Test Site during 1969 and 1970 reveal that the Ektachrome Infrared film was superior to Ektachrome Aerographic, Infrared Aerographic, or Plus-X Aerographic films for detecting Fomes annosus damage. Of far more significance in controlling the accuracy of damage detection, however, was the experience of the photo interpreter. Ratio-processing of multispectral scanner data was investigated with data collected in June of 1970 and in June of 1972. Ratioing of the 1.5-1.8 and 1.0-1.4 micrometer channels gave good results at detecting openings in the crown canopy and adjacent infected trees. Combined level slicing of the 1.5-1.8 micrometer channel and the 1.5-1.8 to 1.0-1.4 micrometer ratio permitted separation and recognition of forest litter in the openings and stressed trees adjacent ot the openings.

Olson, C. F., Jr.↗

NASA NTRS1989

Mars Rover Sample Return: A sample collection and analysis strategy for exobiology

For reasons defined elsewhere it is reasonable to search for biological signatures, both chemical and morphological, of extinct life on Mars. Life on Earth requries the presence of liquid water, therefore, it is important to explore sites on Mars where standing bodies of water may have once existed. Outcrops of layered deposits within the Valles Marineris appear to be ancient lake beds. Because the outcrops are well exposed, relatively shallow core samples would be very informative. The most important biological signature to detect would be organics, microfossils, or larger stromato-like structures, although the presence of cherts, carbonates, clays, and shales would be significant. In spite of the limitations of current robotics and pattern recognition, and the limitations of rover power, computation, Earth communication bandwidth, and time delays, a partial scenario was developed to implement such a scientific investigation. The rover instrumentation and the procedures and decisions and IR spectrometer are described in detail. Preliminary results from a collaborative effort are described, which indicate the rover will be able to autonomously detect stratification, and hence will ease the interpretation burden and lead to greater scientific productivity during the rover's lifetime.

Sims, M. H.↗

DOE OSTI2026

Local Chain Dynamics in Sequence-Controlled Polymers as a Tunable Handle for Rare Earth Sequestration

Chain dynamics govern the intricate behaviors of proteins, underpinning functions such as catalysis, recognition, and stimulus response, and are an increasingly appreciated aspect of structure–function relationships. Analogously, manipulating chain dynamics and structure in abiotic polymers via sequence control is an exciting, yet underexplored, strategy for improving material functions. In this work, we report a systematic study relating the sequence of polymeric sequestrants to their structure and dynamics, as well as to their binding affinity and selectivity for model substrates, rare earth elements (REEs). A series of sequence-controlled polymers with metal chelating, solubilizing, and structure forming monomers was synthesized via multiblock polymerization, yielding compositionally identical polymers with spectroscopically resolved domains and distinct morphologies. Using a combination of small-angle X-ray scattering and 19 F NMR relaxometry measurements, we connected differences in polymer structure and dynamics to polymer sequence variables such as the patchiness (density) of the structure forming monomer and the location of the chelating monomer. Furthermore, we found that, relative to calcium, all polymers in the series collapse more and have slower dynamics when binding REEs (lanthanum and lutetium) , though the extent of these effects were sequence-dependent and localized to specific domains within the polymer. Notably, sequence-controlled polymers that exhibited the largest conformational and dynamic changes upon binding REEs also bound REEs with the greatest affinity and modest selectivity. Collectively, these results correlate monomer patterning with dynamics, morphology, and REE binding performance en route to the development of efficient and selective macromolecular chelators.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2021

Head on Comparison of Self- and Nano-Assemblies of Gamma Peptide Nucleic Acid Amphiphiles

Peptide nucleic acids (PNAs) are nucleic acid analogs with hybridization properties and enzymatic stability superior to that of DNA. In addition to gene targeting applications, PNAs have garnered significant attention as bio-polymers due to the Watson–Crick-based molecular recognition and flexibility of synthesis. Here, in this work, PNA amphiphiles are engineered using chemically modified gamma PNA (8 mer in length) containing hydrophilic diethylene glycol units at the gamma position and covalently conjugated lauric acid (C12) as a hydrophobic moiety. Gamma PNA ($\gamma$PNA) amphiphiles self-assemble into spherical vesicles. Further, nano-assemblies (NA) are formulated using the amphiphilic $\gamma$PNA as a polymer via ethanol injection-based protocols. Comprehensive head-on comparison of the physicochemical and cellular uptake properties of PNA derived self- and NA is performed. Small-angle neutron and X-ray scattering analysis reveal ellipsoidal morphology of $\gamma$PNA NA that results in superior cellular delivery compate to the spherical self-assembly. Next, the functional activities of $\gamma$PNA self-and NA in lymphoma cells via multiple endpoints, including gene expression, cell viability, and apoptosis-based assays are compared. Overall, it is established that $\gamma$PNA amphiphile is a functionally active bio-polymer to formulate NA for a wide range of biomedical applications.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1988

Risk to civilization: A planetary science perspective

One of the most profound changes in our perspective of the solar system resulting from the first quarter century of planetary exploration by spacecraft is the recognition that planets, including Earth, were bombarded by cosmic projectiles for 4.5 aeons and continue to be bombarded today. Although the planetary cratering rate is much lower now than it was during the first 0.5 aeons, sizeable Earth-approaching asteroids and comets continue to hit the Earth at a rate that poses a finite risk to civilization. The evolution of this planetary perspective on impact cratering is gradual over the last two decades. It took explorations of Mars and Mercury by early Mariner spacecraft and of the outer solar system by the Voyagers to reveal the significance of asteroidal and cometary impacts in shaping the morphologies and even chemical compositions of the planets. An unsettling implication of the new perspective is addressed: the risk to human civilization. Serious scientific attention was given to this issue in July 1981 at a NASA-sponsored Spacewatch Workshop in Snowmass, Colorado. The basic conclusion of the 1981 NASA sponsored workshop still stands: the risk that civilization might be destroyed by impact with an as-yet-undiscovered asteroid or comet exceeds risk levels that are sometimes deemed unacceptable by modern societies in other contexts. Yet these impact risks have gone almost undiscussed and undebated. The tentative quantitative assessment by some members of the 1981 workshop was that each year, civilization is threatened with destruction with a probability of about 1 in 100,000. The enormous spread in risk levels deemed by the public to be at the threshold of acceptability derives from a host of psychological factors that were widely discussed in the risk assessment literature. Slovic shows that public fears of hazards are greatest for hazards that are uncontrollable, involuntary, fatal, dreadful, globally catastrophic, and which have consequences that seem inequitable, especially if they affect future generations.

Chapman, Clark R.↗