Results for “NASA SPACE RADIATION LABORATORY” — Page 3

NASA NTRSDate not supplied

HRP Data Management Plan

The purpose of Human Research Program Data Management Plan (DMP) is to define the processes and activities required for the overall management of the research data collected and managed by HRP throughout their life cycle. New updates to the Data Management Plan in 2023 include 1. CAPABILITIES AND SERVICES Data Repositories. Principal Investigators (PIs) funded by HRP may be asked to submit data to one of several NASA data repositories. HRP archives data in the NASA Life Sciences Portal (NLSP) that it considers to be unique and high value. This includes data from human subjects in space flight (ISS and commercial flights) and ground analogs to spaceflight; spaceflight tech demos involving humans; human omics data including the microbiome; parabolic flight studies; and the NASA Space Radiation Laboratory (NSRL). The Open Science Data Repository (OSDR) includes The Ames Life Sciences Data Archive (ALSDA), used to archive non-human biological data (e.g., animal) generated by the Human Research program, and GeneLab, available to HRP PIs to archive non-human omics data. Catalog for search and retrieval. A catalog of non-human HRP life science experiments, with all associated descriptions (mission, payload, hardware, and personnel related information), and biospecimens is provided on the NLSP public web site for search and retrieval. 2. IRB ROLE IN RETURN OF INDIVIDUAL RESEARCH RESULTS The NASA IRB manages the process for incidental findings and for returning results to subjects for studies for which NASA IRB is the IRB of record. Omics data, especially genomics data, may generate information significant to the health of or risk to a research subject. These data potentially hold the keys to understand lifetime risks of chronic diseases, such as cancer, as well as risks associated with exposures common in space flight. 3. UPDATE OF TERMS – IDENTIFIABLE AND ATTRIBUTABLE DATA HRP now follows Federal and NASA policy by using “identifiable” instead of “attributable” for Personally Identifiable Information (PII). 4. POLICY ABOUT INTERNAL NON-RESEARCH USE OF DATA The HRP Chief Scientist grants access to data from HRP-funded research for non-research internal use that includes program management, customer facilitation, strategic planning, and risk research planning. Typical HRP personnel granted access to HRP research data for internal use include the Element Scientist, Subject Matter Experts (SME), and Data/bioinformatics Scientists. If data accessed for Internal Use is provided to an intramural or extramural scientist for hypothesis driven research, all Federal and NASA regulations (e.g., IRB review) regarding human subject research apply.

Data Management Plan↗

NASA NTRSDate not supplied

Oxidative Stress and the Neuroconsequences of Spaceflight Environment - Immune Dysregulation and Antioxidant Dietary Countermeasure Efficacy

In this project, we will test the hypothesis that Ionizing Radiation (IR), microgravity and social isolation combine synergistically to trigger an oxidative stress response that alters immune homeostasis, brain structure and function, and neurobehavioral and cognitive performance. Specific Aims for this project are: (1) Determine dose-response curves for acute ‘Five-Ion GCR Simulation’ exposure for immune, brain and performance responses in crew age-matched adult male and female mice; (2) Determine effects of acute ‘Five-Ion GCR Simulation’ exposure singly and in combination with simulated microgravity and social isolation, on immune, brain and performance responses in crew age-matched male and female mice mimicking deep space missions; and (3) Determine efficacy of the dietary antioxidant, Nicotinamide Mononucleotide (NMN), a key intermediate in nicotinamide adenine dinucleotide (NAD+) biosynthesis. The project relies on established and highly translatable ground-based mouse models and assays with IR exposures to be performed at the NASA Space Radiation Laboratory (NSRL). The experimental approach will provide definitive data on the timing and mechanisms involved in the oxidative stress response, immune and brain changes, and ensuing functional (behavioral/cognitive) impairments expected during human transit to Mars. This project will identify potential biomarkers for, and mechanisms underlying, structural and functional changes in the immune and nervous systems leading to behavioral/cognitive performance deficits, and its potential application to develop effective countermeasures to mitigate negative health effects of long duration space habitation. The project will address NASA’s efforts to rapidly advance the characterization of risks and identify appropriate countermeasures in anticipation of future deep space missions. Ensuring crew health and performance during extended transits necessitates that sensorimotor and cognitive abilities remain strong to avoid potentially catastrophic health and safety outcomes. Further, despite historically low numbers of female astronauts, the two most recent NASA Astronaut Corps class selections, comprised of ~50% women as compared to men, signal the need to understand how sex differences affect physiological adaptation and health in the space environment. Here, we will determine how key features of the deep space environment may interact to increase risk to crewmembers by negatively impacting health and performance, and identify and develop strategies to characterize and mitigate the potential risks via countermeasures. Supported by the NASA Human Research Program (HRP) Human Factors Behavioral Performance Element Grant 18-18FLAG_2-0028.

radiation↗

DOE OSTI2025

Commissioning of Extended Electron Beam Ion Source at Brookhaven National Laboratory

Here, the Extended Electron Beam Ion Source (EEBIS) has been installed and commissioned at Brookhaven National Laboratory (BNL) hadron accelerator complex in the spring of 2023. EEBIS has replaced its predecessor, RhicEBIS, which provided ions for BNL hadron facilities for over a decade since 2009. The motivations for the source upgrade are to provide higher intensities of the Au 32+ ion beam for the Relativistic Heavy Ion Collider (RHIC) and to provide of an intense source of polarized 3 He 2+ ions for the future Electron Ion Collider (EIC) at BNL. EEBIS is and will continue to be the primary source for a variety of different ion species for the NASA Space Radiation Laboratory (NSRL) at BNL. EEBIS utilizes two identical, two-meter-long unshielded 5T warm bore superconducting solenoids, and it is operated with electron beam current up to 10 A, providing a high-capacity ion traps to fulfill the requirement for high intensity ion beams. The upstream solenoid contains a “short ion trap” with a length of 95 cm, while the “long ion trap” with a length of 178 cm is located inside the downstream solenoid bore. The main features of EEBIS are: * A Gas injection and ionization cell equipped with a Lorenz pulse valve * High-capacity ZAO non-evaporable getter (NEG) custom linear pumping units * In situ apparatus for pumping speed measurements to monitor NEG activation and saturation * “External drift tube” construction with differential pumping stages to provide space for Lorentz pulse valve and 3 He high-field polarization cell * A Ba oxide electron gun cathode. The design of EEBIS as well as the results of its commissioning and first-year operation are presented and discussed.

43 PARTICLE ACCELERATORS↗

NASA NTRSDate not supplied

NASA's Space Health Impacts for the NASA Experience (SHINE) Training Program – Space Radiation Curriculum

In February 2023, the Space Radiation Element of the NASA Human Research Program initiated a virtual, annual space radiation curriculum. The Space Health Impacts for the NASA Experience (SHINE) Space Radiation Didactic Curriculum aims to educate participants not only in the scientific aspects of space radiation but also in the agency’s risk management strategies. SHINE Space Radiation Didactic Curriculum combined weekly seminars by speakers from NASA, other government agencies, academia, and industry, with networking sessions designed to foster collaboration between the competitively selected participants as well as interactions with NASA scientists and HRP funded investigators. The inaugural course ran weekly from February 2023 to August 2023 and was comprised of lectures, less formal coffee hours, and office hours. Each two-hour seminar sessions hosted 1-3 presentations on topics which ranged from the space radiation environment to health effects and countermeasure to granting opportunities. All lectures were recorded and will be published on the THREE (The Health Risks of Extraterrestrial Environments) website for public access (https://three.jsc.nasa.gov/). As a course requirement, participants developed individual or collaborative beam time proposals for real, proposed, or potential experiments at the NASA Space Radiation Laboratory (NSRL) at the Brookhaven National Laboratory. For the 2023 course, 25 participants were selected from a total of 59 applicants. Selected participants were citizens from 8 countries and comprised 5 graduate students, 4 postdocs, 11 scientists and 5 professors/medical doctors. Participants had a wide range of expertise including molecular and cellular biology, microbiology, botany, physiology, engineering, physics, aerospace medicine, planetary science, biostatistics, and modeling. The second annual SHINE training program is scheduled from February to August 2024. In addition, a separate SHINE Space Radiation Practicum session will be held in Fall 2024 at the NSRL. The SHINE Space Radiation Practicum is a unique opportunity that has not been available to the public since the closure of the NASA Space Radiation Summer School in 2017 and will allow a small cohort of participants competitively selected in Fall 2023 to gain hands-on radiation experience.

SHINE↗

NASA NTRSDate not supplied

The NASA Biological Institutional Scientific Collection (NBISC): Tissue and Microbe Biospecimens to Advance Space Research

The NASA Biological Institutional Scientific Collection (NBISC) is a biorepository of non-human biospecimens from NASA-funded spaceflight investigations and correlative ground studies. The collection has its roots in the 1960s through collaborations by NASA Ames Research Center with other NASA centers, universities, and international space agencies by sharing non-human biospecimens from spaceflight and space-relevant ground experiments. These collaborations have advanced the field of space exploration by helping to maximize the data gained from spaceflight and other NASA-funded experiments. NBISC coordinates closely with the Biospecimen Sharing Program (BSP) which is responsible for organized sample collection from spaceflight and ground rodent experiments. In 2022 NBISC partnered with HRP’s Biospecimen and Tissue Sharing Collection (BTSC) Program to also archive samples from HRP’s Space Radiation Element-funded studies which primarily involve rodents exposed to various radiation protocols at the NASA Space Radiation Laboratory (NSRL) and other analog facilities. To date, more than fifty thousand biospecimens from BTSC have been transferred to NBISC for distribution to the community. In 2023, NBISC embarked on a new Space Biology funded endeavor to create the Space Microbial Culture Collection (SMCC) which will serve as a central repository for microbial isolates associated with space flight, gravitational, and space radiation research. This presentation will highlight several success stories of analyses carried out using archived NBISC samples received by researchers in recent years and will detail the process for proposing and receiving samples from NBISC, BTSC and SMCC. Making available these 150,000+ unique biospecimens to the scientific research community, NBISC not only serves as a repository for storing and distributing non-human biospecimens, but also acts as a resource to enable new discoveries that will benefit NASA and humankind.

NBISC↗

NASA NTRSDate not supplied

The NASA Biological Institutional Scientific Collection (NBISC): Tissue and Microbe Biospecimens to Advance Space Research

The NASA Biological Institutional Scientific Collection (NBISC) is a biorepository of non-human biospecimens from NASA-funded spaceflight investigations and correlative ground studies. The collection has its roots in the 1960s through collaborations by NASA Ames Research Center with other NASA centers, universities, and international space agencies by sharing non-human biospecimens from spaceflight and space-relevant ground experiments. These collaborations have advanced the field of space exploration by helping to maximize the data gained from spaceflight and other NASA-funded experiments. NBISC coordinates closely with the Biospecimen Sharing Program (BSP) which is responsible for organized sample collection from spaceflight and ground rodent experiments. In 2022 NBISC partnered with HRP’s Biospecimen and Tissue Sharing Collection (BTSC) Program to also archive samples from HRP’s Space Radiation Element-funded studies which primarily involve rodents exposed to various radiation protocols at the NASA Space Radiation Laboratory (NSRL) and other analog facilities. To date, more than fifty thousand biospecimens from BTSC have been transferred to NBISC for distribution to the community. In 2023, NBISC embarked on a new Space Biology funded endeavor to create the Space Microbial Culture Collection (SMCC) which will serve as a central repository for microbial isolates associated with space flight, gravitational, and space radiation research. This presentation will highlight several success stories of analyses carried out using archived NBISC samples received by researchers in recent years and will detail the process for proposing and receiving samples from NBISC, BTSC and SMCC. Making available these 150,000+ unique biospecimens to the scientific research community, NBISC not only serves as a repository for storing and distributing non-human biospecimens, but also acts as a resource to enable new discoveries that will benefit NASA and humankind.

Biospecimen↗

NASA NTRSDate not supplied

Changes in Plants Developed from Imbibed Seeds Irradiated by Simulated Solar Particle Events

One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is radiation risk primarily from solar particle events (SPEs) and galactic cosmic rays (GCRs). With the goal of manned Mars exploration, the production of fresh food during long duration space missions may provide nutritional supplementation and benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of simulated GCRs on dry seeds of mizuna mustard, ‘Outredgeous’ red romaine lettuce, and ‘Red Robin’ dwarf tomato. Seeds were exposed to various doses of simulated GCRs (0, 40, and 80 cGy), either acutely or at a low dose rate (LDR), using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Control and irradiated seeds were planted and maintained under space-vehicle relevant controlled environment conditions. Plant/fruit morphometrics and edible fresh mass were measured. In addition, nutrient analyses were performed for both mizuna and ripe tomatoe fruit. Seedlings from irradiated seeds (both 40 cGy and 80 cGy) showed significant changes in cotyledon development, but not in root length or morphology. Interestingly, significant changes in vitamin content were found in mizuna grown from 80 cGy (LDR) irradiated seeds. In addition, 80 cGy (LDR) simulated GCRs significantly affected lettuce and tomato seed quality, delayed tomato fruiting, and reduced the total yield of tomato. Generally, compared to 80 cGy, 40 cGy (LDR) of simulated GCRs displayed some impacts to the seeds and seedlings, but to a much lesser degree. (This study was supported by NASA Human Research Program.)

J.T. Richards↗

NASA NTRSDate not supplied

Changes in Plants Developed from Imbibed Seeds Irradiated by Simulated Solar Particle Events

One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is radiation risk primarily from solar particle events (SPEs) and galactic cosmic rays (GCRs). With the goal of enabling manned Mars exploration, the production of fresh food during long duration space missions may provide nutritional supplementation and benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of simulated SPEs on imbibed seeds of mizuna mustard, ‘Outredgeous’ red romaine lettuce, and ‘Red Robin’ dwarf tomato. Seeds were exposed to various doses of simulated SPEs (0, 40, 80 cGy, and 200 cGy), either acutely or at a low dose rate (LDR only for 40 and 80 cGy doses), using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Simulated SPE exposure was achieved using sequential exposures to protons with a broad energy range at 50-150 MeV/n. Control and irradiated seeds were planted either onto 0.5% MS-based agar plates containing required nutrients for early seedling analysis, or in peat plugs/pots for 28 day (mizuna and lettuce) and 91 day (tomato) grow outs. All plants were maintained under space-vehicle relevant conditions at 3000 ppm CO2, 50% RH, and 23°C, and a 16/8 h photoperiod with 300 μmol·m(exp -2)·s(exp -1) photosynthetically active radiation. Plant/fruit morphometrics and edible fresh mass were measured. Additionally, nutrient analyses were performed on mizuna and tomato fruit. Seedlings from irradiated seeds (both 40 and 80 cGy) showed significant and dose dependent impacts on cotyledon development and root length. Interestingly, small, but significant changes in vitamin content were found in mizuna and tomato fruits grown from 80 cGy (LDR) irradiated seeds. In addition, 80 cGy (LDR) simulated SPEs significantly delayed tomato early seedling development and plant growth, and reduced the quantity of fruits produced from the surviving plants. (This study was supported by NASA Human Research Program.)

J. T. Richards↗

NASA NTRSDate not supplied

Impact of Space Radiation on Plants: from Arabidopsis thaliana to Crops

One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is radiation risk, primarily from solar particle events (SPE) and galactic cosmic rays (GCR). With the goal of manned Mars exploration, the production of fresh food during long-duration space missions provides critical nutritional supplementation and may also benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of simulated GCR (using dry seeds) or SPE (using hydrated seeds) on seeds of Arabidopsis, Mizuna mustard, ‘Outredgeous’ red romaine lettuce, and ‘Red Robin’ dwarf tomato. Seeds were exposed to various doses of simulated space radiation scenarios, either acutely or at a low dose rate (LDR), using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Exposure to simulated GCR or SPE at the levels tested had no significant impact on the germination rate in Arabidopsis and crop seeds; however, GCR reduced the viability of lettuce and tomato seeds. Overall, the morphological changes of the seedlings cultured from irradiated seeds were dose- and ion quality- dependent, with heavier ions causing more severe damage. These changes ranged from cotyledon deformation, shortened root length, smaller seedling size, and other signs of stress, depending on the seed types. Both 40 and 80 cGy (LDR) exposures of GCR or SPE significantly affected tomato early seedling development, delayed tomato fruiting, and reduced the total yield of tomato. Altered nutritional values were also found in edible biomass, especially for the GCR 80 cGy (LDR) groups. For both GCR and SPE, 40 cGy showed some effects, but to a much lesser extent compared with 80 cGy, which can be considered as the “maximum permissible exposure” for the seed types we evaluated in this study. The impact of space radiation on seeds potentially affects the ability of plants to adapt to other environmental stresses (e.g. microgravity, water stress, and hardware constraints) as well as susceptibility to plant diseases, which need to be furtherly investigated. This research is funded by NASA’s Human Research Program.

J T Richards↗

NASA NTRSDate not supplied

Impact of Space Radiation on Plant Seeds: from Arabidopsis thaliana to Crops

One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is radiation risk, primarily from solar particle events (SPE) and galactic cosmic rays (GCR). With the goal of manned Mars exploration, the production of fresh food during long-duration space missions provides critical nutritional supplementation and may also benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of simulated GCR (using dry seeds) or SPE (using hydrated seeds) on seeds of Arabidopsis, Mizuna mustard, ‘Outredgeous’ red romaine lettuce, and ‘Red Robin’ dwarf tomato. Seeds were exposed to various doses of simulated space radiation scenarios, either acutely or at a low dose rate (LDR), using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Exposure to simulated GCR or SPE at the levels tested had no significant impact on the germination rate in Arabidopsis and crop seeds; however, GCR reduced the viability of lettuce and tomato seeds. Overall, the morphological changes of the seedlings cultured from irradiated seeds were dose- and ion quality- dependent, with heavier ions causing more severe damage. These changes ranged from cotyledon deformation, shortened root length, smaller seedling size, and other signs of stress, depending on the seed types. Both 40 and 80 cGy (LDR) exposures of GCR or SPE significantly affected tomato early seedling development, delayed tomato fruiting, and reduced the total yield of tomato. Altered nutritional values were also found in edible biomass, especially for the GCR 80 cGy (LDR) groups. For both GCR and SPE, 40 cGy showed some effects, but to a much lesser extent compared with 80 cGy, which can be considered as the “maximum permissible exposure” for the seed types we evaluated in this study. The impact of space radiation on seeds potentially affects the ability of plants to adapt to other environmental stresses (e.g. microgravity, water stress, and hardware constraints) as well as susceptibility to plant diseases, which need to be furtherly investigated. This research is funded by NASA’s Human Research Program.

J T Richards↗

NASA NTRSDate not supplied

Changes in Plants Developed from Imbibed Seeds Irradiated by Simulated Solar Particle Events

One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is radiation risk primarily from solar particle events (SPEs) and galactic cosmic rays (GCRs). With the goal of manned Mars exploration, the production of fresh food during long duration space missions may provide nutritional supplementation and benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of simulated SPEs on imbibed seeds of mizuna mustard, ‘Outredgeous’ red romaine lettuce, and ‘Red Robin’ dwarf tomato. Seeds were exposed to various doses of simulated SPEs (0, 40, 80 cGy, and 200 cGy), either acutely or at a low dose rate (LDR only for 40 and 80 cGy doses), using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Simulated SPE exposure was achieved using sequential exposures to protons with a broad energy range at 50-150 MeV/n. Control and irradiated seeds were planted either onto 0.5% MS-based agar plates containing required nutrients for early seedling analysis, or in peat plugs/pots for 28 day (mizuna and lettuce) and 91 day (tomato) grow outs. All plants were maintained under space-vehicle relevant controlled environment conditions at 3000 ppm CO2, 50% RH, and 23°C, and a 16/8 h photoperiod with 300 μmol·m-2·s-1 photosynthetically active radiation. Plant/fruit morphometrics and edible fresh mass were measured. In addition, nutrient analyses were performed for both mizuna and ripe tomatoe fruit. Seedlings from irradiated seeds (both 40 cGy and 80 cGy) showed significant changes in cotyledon development, as well as root length. Interestingly, small, but significant changes in vitamin content were found in mizuna and tomato fruits grown from 80 cGy (LDR) irradiated seeds. In addition, 80 cGy (LDR) simulated SPEs significantly affected tomato early seedling development, plant growth, and the quantity of fruits produced from the surviving plants. Generally, compared to 80 cGy, 40 cGy (LDR) of simulated SPEs displayed some impacts to the seeds and seedlings, but to a much lesser degree. (This study was supported by NASA Human Research Program.)

J.T. Richards↗

NASA NTRSDate not supplied

Impact of Space Radiation on Plant Seeds: from Arabidopsis thaliana to Crops

One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is radiation risk, primarily from solar particle events (SPE) and galactic cosmic rays (GCR). With the goal of manned Mars exploration, the production of fresh food during long-duration space missions provides critical nutritional supplementation and may also benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. This is the final report of our project to summarize the findings of the effect of space radiation exposure on plant seeds from morphometrics, nutritional values, to molecular machenisms. In this study, we evaluated the effect of simulated GCR (using dry seeds) or SPE (using hydrated seeds) on seeds of Arabidopsis, Mizuna mustard, ‘Outredgeous’ red romaine lettuce, and ‘Red Robin’ dwarf tomato. Seeds were exposed to various doses of simulated space radiation scenarios, either acutely or at a low dose rate (LDR), using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Exposure to simulated GCR or SPE at the levels tested had no significant impact on the germination rate in Arabidopsis and crop seeds; however, GCR reduced the viability of lettuce and tomato seeds. Overall, the morphological changes of the seedlings cultured from irradiated seeds were dose- and ion quality- dependent, with heavier ions causing more severe damage. These changes ranged from cotyledon deformation, shortened root length, smaller seedling size, and other signs of stress, depending on the seed types. Both 40 and 80 cGy (LDR) exposures of GCR or SPE significantly affected tomato early seedling development, delayed tomato fruiting, and reduced the total yield of tomato. Altered nutritional values were also found in edible biomass, especially for the GCR 80 cGy (LDR) groups. Underlying mechanisms were furtherly evaluated using transcriptomic analysis. For both GCR and SPE, 40 cGy showed some effects, but to a much lesser extent compared with 80 cGy, which can be considered as the “maximum permissible exposure” for the seed types we evaluated in this study. The impact of space radiation on seeds potentially affects the ability of plants to adapt to other environmental stresses (e.g. microgravity, water stress, and hardware constraints) as well as susceptibility to plant diseases, which need to be furtherly investigated. This research is funded by NASA’s Human Research Program.

J T Richards↗

NASA NTRSDate not supplied

Impact of Space Radiation on Plant Seeds: from Arabidopsis thaliana to Crops

One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is radiation risk, primarily from solar particle events (SPE) and galactic cosmic rays (GCR). With the goal of manned Mars exploration, the production of fresh food during long-duration space missions provides critical nutritional supplementation and may also benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. This is the final report of our project to summarize the findings of the effect of space radiation exposure on plant seeds from morphometrics, nutritiaonal values, to molecular machenisms. In this study, we evaluated the effect of simulated GCR (using dry seeds) or SPE (using hydrated seeds) on seeds of Arabidopsis, Mizuna mustard, ‘Outredgeous’ red romaine lettuce, and ‘Red Robin’ dwarf tomato. Seeds were exposed to various doses of simulated space radiation scenarios, either acutely or at a low dose rate (LDR), using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Exposure to simulated GCR or SPE at the levels tested had no significant impact on the germination rate in Arabidopsis and crop seeds; however, GCR reduced the viability of lettuce and tomato seeds. Overall, the morphological changes of the seedlings cultured from irradiated seeds were dose- and ion quality- dependent, with heavier ions causing more severe damage. These changes ranged from cotyledon deformation, shortened root length, smaller seedling size, and other signs of stress, depending on the seed types. Both 40 and 80 cGy (LDR) exposures of GCR or SPE significantly affected tomato early seedling development, delayed tomato fruiting, and reduced the total yield of tomato. Altered nutritional values were also found in edible biomass, especially for the GCR 80 cGy (LDR) groups. Underlying mechanisms were furtherly evaluated using transcriptomic analysis. For both GCR and SPE, 40 cGy showed some effects, but to a much lesser extent compared with 80 cGy, which can be considered as the “maximum permissible exposure” for the seed types we evaluated in this study. The impact of space radiation on seeds potentially affects the ability of plants to adapt to other environmental stresses (e.g. microgravity, water stress, and hardware constraints) as well as susceptibility to plant diseases, which need to be furtherly investigated. This research is funded by NASA’s Human Research Program.

J.T. Richards↗

NASA NTRS2010

Radiation Beamline Testbeds for the Simulation of Planetary and Spacecraft Environments for Human and Robotic Mission Risk Assessment

The Center for Radiation Engineering and Science for Space Exploration (CRESSE) at Prairie View A&M University, Prairie View, Texas, USA, is establishing an integrated, multi-disciplinary research program on the scientific and engineering challenges faced by NASA and the international space community caused by space radiation. CRESSE focuses on space radiation research directly applicable to astronaut health and safety during future long term, deep space missions, including Martian, lunar, and other planetary body missions beyond low earth orbit. The research approach will consist of experimental and theoretical radiation modeling studies utilizing particle accelerator facilities including: 1. NASA Space Radiation Laboratory (NSRL) at Brookhaven National Laboratory; 2. Proton Synchrotron at Loma Linda University Medical Center; and 3. Los Alamos Neutron Science Center (LANSCE) at Los Alamos National Laboratory. Specifically, CRESSE investigators are designing, developing, and building experimental test beds that simulate the lunar and Martian radiation environments for experiments focused on risk assessment for astronauts and instrumentation. The testbeds have been designated the Bioastronautics Experimental Research Testbeds for Environmental Radiation Nostrum Investigations and Education (BERT and ERNIE). The designs of BERT and ERNIE will allow for a high degree of flexibility and adaptability to modify experimental configurations to simulate planetary surface environments, planetary habitats, and spacecraft interiors. In the nominal configuration, BERT and ERIE will consist of a set of experimental zones that will simulate the planetary atmosphere (Solid CO2 in the case of the Martian surface.), the planetary surface, and sub-surface regions. These experimental zones can be used for dosimetry, shielding, biological, and electronic effects radiation studies in support of space exploration missions. BERT and ERNIE are designed to be compatible with the experimental areas associated with the above facilities. CRESSE has broad expertise in space radiation in the areas of space radiation environment modeling, Monte-Carlo radiation transport modeling, space radiation instrumentation and dosimetry, radiation effects on electronics, and multi-functional composite shielding materials. The BERT and ERNIE testbeds will be utilized in individual and collaborative research incorporating this expertise. The research goal is to maximize the technical readiness level (TRL) of radiation instrumentation for human and robotic missions, optimizing the return value of CRESSE for NASA exploration and international co-operative missions. Outcomes and knowledge from research utilizing BERT and ERNIE will be applied to a variety of scientific and engineering disciplines vital for safe and reliable execution of future space exploration missions, which can be negatively impacted by the space radiation environment. The testbeds will be central to a variety of university educational activities and educational goals of NASA. Specifically, BERT and ERNIE will enhance educational opportunities in science, technology, engineering and mathematics (STEM) disciplines for engineering and science students at PVAMU, a historically black college/university. Preliminary data on prototype testbed configurations, including simulated lunar regolith (JSC-1A stimulant based on Apollo 11 samples), regolith/polyethylene composites, and dry ice, will be presented to demonstrate the usefulness of BERT and ERNIE in radiation beam line experiments.

Wilkins, Richard↗

DOE OSTI2018

Light charged ion measurements and Monte Carlo calculations from thick targets bombarded by protons and heavy ions - 25435

The data presented in this work are from experiments performed in November 2016 and November 2017 at the National Aeronautics and Space Administration (NASA) Space Radiation Laboratory at Brookhaven National Laboratory (BNL). The secondary light charged ions (Z=1 and 2 ions) and neutrons produced by 400-, 800-, and 2500-AMeV protons and 400-, 800-, and 1500-AMeV He, C, Si, Fe ions bombarding thick targets (20, 40, and 60 g/cm{sup 2} aluminum and high-density polyethylene (HDPE), and 20 and 60 g/cm{sup 2} combination of aluminum and HDPE) were measured with organic liquid scintillators and sodium iodide (NaI) detectors. The organic liquid scintillators were positioned at 10 deg., 30 deg., 45 deg., 60 deg., 80 deg., and 135 deg. with respect to the beam direction, while NaI detectors were placed at 10 deg. and 30 deg. off the beam axis. The double-differential thick-target secondary light charged ion yields measured by organic liquid scintillators are of main interest here. In the analysis, after applying pulse shape discrimination to separate protons, deuterons, tritons, helium-3, and helium-4 particles, the time of flight technique was used to produce the secondary charged particle angular-energy spectra. Additionally, the spectra were compared with simulation results using the Monte Carlo simulation code, PHITS version 2.88. To obtain favorable statistics in PHITS calculations, ring-type detectors were modeled, which have the same distances and equivalent angles with the experimental setup. These experimental data will be integrated into the uncertainty analysis for NASA to optimize future GCR shielding design. (authors)

43 PARTICLE ACCELERATORS↗

DOE OSTI2021

Impact of galactic cosmic ray simulation on nutritional content of foods

Foods packaged for future deep-space exploration missions may be prepositioned ahead of astronaut arrival, and will be exposed to galactic cosmic rays (GCRs) and solar radiation in deep space at higher levels and different spectrums than those found in low-Earth orbit (LEO). In this study, we have evaluated the impact of a GCR simulation (approximately 0.5 and 5 Gy doses) at the NASA Space Radiation Laboratory (NSRL) on two retort thermostabilized food products that are good sources of radiation labile nutrients (thiamin, vitamin E, or unsaturated fats). No trends or nutritional differences were found between the radiation-treated samples and the control immediately after treatment or one-year after treatment. Small changes in a few nutrients were measured following one-year of storage. Further studies are needed to confirm these results, as the foods in this study were heterogeneous, and this may have masked meaningful changes due to pouch-to-pouch variations.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2019

Cluster ion source for external injection and high capacity filling of light elements into the relativistic heavy ion collider electron beam ion source

An advanced Electron Beam Ion Source (EBIS) is the primary ion source to supply highly charged ion beams of different elements to the Relativistic Heavy Ion Collider (RHIC) and to the NASA Space Radiation Laboratory (NSRL). Intense beams of highly charged ions of various elements of the periodic table, ranging from helium to uranium, have been demonstrated since EBIS became operational in 2010. EBIS routinely provides ion beams to RHIC and NSRL quasisimultaneously with about 1 s switching time between different ion species. Such unique flexibility and rapid switching between ion species are based on external injection of singly charged ions into the EBIS trap either in “fast” or “slow” injection modes. At present, a Laser Ion Source (LIS) provides most of the ion species of solid materials using the “fast” injection mode into the EBIS trap and a Hollow Cathode Ion Source (HCIS) provides most of the ion species of gaseous elements using the “slow” injection mode into the EBIS trap. Gas injection into the EBIS trap is also possible and has been used but imposes some restrictions for the simultaneous generation of highly charged ions such as Au 32+ ions for RHIC and ions of gaseous species for NSRL. Because light ions have relatively high velocity inside the EBIS trap, efficient injection of hydrogen and helium ions and filling of the EBIS trap to high capacity is difficult from either LIS or HCIS. To overcome this restriction and enhance EBIS operational capability, we suggest injecting beams of hydrogen and helium cluster ions into the EBIS trap. Required parameters of cluster ion beam injection into the EBIS trap are estimated, and advantages of such an injection are highlighted. A cluster ion source with required high intensity is visible and will be designed, built, optimized, and tested.

43 PARTICLE ACCELERATORS↗

DOE OSTI2020

Beam characterization at NSRL for radiobiological experiments—phase 1

An experimental campaign was carried out at the NASA Space Radiation Laboratory to perform an additional, independent dosimetric characterization of the beams of protons, helium and carbon ions for radiobiological experiments. The campaign was undertaken by the request and with the support from the National Cancer Institute, U.S. In this initial phase, the goals were to obtain a first assessment of the dosimetric reproducibility of the beam control system, including analysis of spatial homogeneity and evaluation of ion beam contamination. They should facilitate the design of further experimental campaigns for beam characterization for radiobiological experiments. Measurements included reference dosimetry with comparison of in-house and external ionization chambers and electrometers, lateral-dose profile measurements in air, depth-dose profile in a water tank, evaluation of water equivalent thickness of a HDPE binary range shifter and estimation of impurities of the investigated helium-ion beam. The experiments and results are presented.

43 PARTICLE ACCELERATORS↗