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Final Report - Hypergravity Effects on Chromatin Conformation and Nuclear Structure in Cultured Cells using High-throughput Sequencing

The conformation of the human genome is known to play an important role in transcriptional control of gene expression. Our aim is to assess whether exposure of cultured human cardiomyocytes to hypergravity can induce changes in chromatin organization as assayed by Hi-C proximity ligation technique. Hi-C is used to analyze chromatin interactions by using formaldehyde to crosslink regions of chromatin that are in close proximity. The DNA is then fragmented using a restriction enzyme and ligated under dilute conditions to favor intramolecular ligation of cross-linked fragments. Finally, the DNA is sequenced, allowing reconstruction of genomic structure. iCell cardiomyocyte cultures will be exposed to 40 g via an engineered device (incu-fuge) that allows for the chemical fixation of cells on a spinning centrifuge. Additionally, we plan on using fluorescence microscopy to analyze the nuclear and actin cytoskeletal conformation of cardiomyocytes post hypergravity exposure. We hypothesize that chromatin will rearrange in hypergravity conditions, both rapidly due to direct mechanical forces, as well as over longer time frames due to changes to structures necessary for transcriptional responses such as the de novo formation of promoter-enhancer loops. A negative result–no change between 1 g and hypergravity conditions–would nevertheless be an important data point in our understanding of the rheology of the nucleus, and how cells and cellular structures respond to different gravity fields. The cellular physiology of hypergravity has clinical relevance for spaceflight, and can further inform our understanding of microgravity physiology.

chromatin

NGC 1068 - Resolution of nuclear structure in the optical continuum

The HST Planetary Camera has been used to obtain an optical continuum image of the Seyfert galaxy NGC 1068. The image reveals a bright nucleus embedded in an irregular cloudlike structure which is well-differentiated against the background of the galaxy. The nucleus is resolved, with an FWHM of about 0.15 arcsec, or 11 pc. There is no evidence for any unresolved nuclear component. The precise geometry of the scattering region cannot yet be inferred. The cloud surrounding the nucleus is elongated in the NE-SSW direction and has extreme dimensions of 3.5 arcsec x 1.7 arcsec. The brightness centroid is situated 0.4 arcsec SW of the nucleus. It is concluded that the light from the cloud is contributed largely by stars, but that the appearance of the cloud is distinctly dissimilar to what is expected from young or old stellar systems and bears to simple relationship to the numerous features that have heretofore been resolved at other wavelengths.

Lynds, Roger

Nuclear Structure and Decay Data for A=230

The experimental reaction and decay studies producing nuclei in the A=230 mass chain have been reviewed. Data on elements from radon (Z=86) to americium (Z=95) are included, and level and decay schemes are presented for these nuclides. This work supersedes the previous evaluation for this mass chain (2012Br12).

73 NUCLEAR PHYSICS AND RADIATION PHYSICS

Nuclear Structure and Decay Data for A=240

The experimental reaction and decay studies producing nuclei in the A=240 mass chain have been reviewed. Data on elements from uranium (Z=92) to einsteinium (Z=99) are included, and level and decay schemes are presented for these nuclides. Furthermore, this work supersedes the previous evaluation for this mass chain (2008Si25).

73 NUCLEAR PHYSICS AND RADIATION PHYSICS

Nuclear Structure and Decay Data for A=216 Isobars

Experimental data from reaction and decay studies on nuclei with A=216 have been reviewed. Elements included in this review span from mercury (Z=80) to uranium (Z=92). Based on the published data, level and decay schemes are presented for the evaluated nuclides. In conclusion, this work updates and supersedes the previous A=216 evaluation (2007Wu02).

73 NUCLEAR PHYSICS AND RADIATION PHYSICS

Nuclear structure of 157 Sm via β – decay of 157 Pm

Excited states of the neutron-rich nucleus 157 Sm were populated through the β – decay of 157 Pm, which has a tentatively assigned ground-state spin and parity of J π = (5/2 – ). Over 30 levels have been observed, 16 of which are new, and over 45 new γ-ray transitions have been placed in the level scheme. An evolution in the ground-state configurations for N = 95 nuclei from 5/2 – [523] (Er/Yb), to 5/2 + [642] (Dy), to 3/2 – [521] (Sm/Gd) can be explained based on increasing deformation from Z = 70 to Z = 62 and the fact that these three orbitals are energetically close to each other at deformations near β 2 ≈ 0.25 – 0.3. Tentative spin and parity assignments are made for most of the states below 1500 keV based on the decay properties of the levels and using excitation-energy systematics of the various orbitals observed in N = 95 nuclei.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS

Nuclear Structure and Decay Data for A=169 Isobars

Experimental data pertaining to all nuclei with mass number A=169 (Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Hf, Ta, W, Re, Os, Ir, Pt) have been evaluated. Level schemes from both radioactive decay and reaction studies are presented, along with associated tables of experimental data and adopted properties for levels and γ rays. The present evaluation for A=169 supersedes the 2008 evaluation, 2008Ba31, by C.M. Baglin. A few highlights of this evaluation: More extensive work on ε decay from 169W is needed and new experimental work will be required to resolve a discrepancy between the J π values deduced for a 180-keV level in 169Ta based on extensive band structure from (HI,xnγ) work (J π =1/2−) and TDPAD measurements (J=5/2). Low lying states of 169Os were studied via fine structure of 173Pt α decay in 2014ThZZ. The Eαs feeding the g.s. of 169Os in 2008Ba31 are separated well into two consistent groups to feed the g.s. and the newly proposed state at 34.84 keV. Based on the studies of 2014ThZZ and 2021Zh52, the g.s. spin-parity assignment of 169Os has been proposed to be (7/2−) from (5/2−). The 169Ir g.s. half-life and alpha emission branching reported in 2012Th13 from 173Au α decay measurements are preferred over the values in 2005Sc22. The reported half-life value in 2005Sc22 for 169Ir g.s. is discrepant and the research work was carried out in the same lab of 2012Th13.

Basunia, M Shamsuzzoha

New scaling and nuclear structure aspects in heavy-ion fusion reactions

Three new behaviors have been found in comparisons of fusion cross sections for different collision systems. root (1) Replacing the energy E with a scaling one, E scal = (E-V g )/($\sqrt{2}$W g ), is successful for washing out the Coulomb interaction in the spectra of fusion cross sections, where V g and W g are barrier height and width of the single-Gaussian barrier distribution model. (2) In a representation of σE vs the scaling energy, E scal , all data sets display in parallel. Here, the ratio for sigma E from any two fusion systems over the whole range is a constant value. That behavior is also studied in another representation, in which the data sets display as parallel horizontal lines for any heavy-ion fusion system. (3) The constant ratio value is the ratio of parameter products, $R^2_gW_g$, of the two systems; where R g is the barrier radius obtained in the single-Gaussian barrier distribution model. Moreover, when comparing neighboring collision systems at the same E scal , the ratio of sigma is near a constant value within a few percent over the whole range. Thus a quantitative comparison for the fusion enhancement for neighboring systems is developed. The present finding could be beneficial for predicting unmeasured fusion cross sections.

Jiang, C. L. [Argonne National Laboratory (ANL), A

Ellipticals with kinematically distinct cores : HST imaging of the nuclear structure of IC 1459

The elliptical galaxy IC 1459 has one of the strongest counter-rotating core components of any observed elliptical. Here we present Hubble Space Telescope (HST) Planetary Camera images of the center of IC 1459. Before deconvolution, our V band images reveal a bright point source at the galaxy nucleus, and dust near the nucleus. After removal of the central point source, deconvolution and model fitting, we show that the central starlight profile is better fit by a 'cusp' model than an isothermal core model. The photometric properties of the stellar light are comparable to those of other ellipticals without counter-rotating core components. There is an indication of a central stellar disk, although its detection is complicated by the extensive dust. Although the velocity field of the emission-line gas is ordered, the dust distribution is very irregular, and indicates nonequilibrium motions. The irregular dust distribution suggests that material is currently infalling and may be fueling the active nucleus. There is no direct evidence which relates the dust and gas to the peculiar stellar kinematics.

Forbes, Duncan A.

Nuclear shell structure governs short-range nucleon pairing

Atomic nuclei are intricate quantum systems in which nucleons (protons and neutrons) are held together by the strong nuclear force. At very short distances, nucleons can momentarily form high-momentum pairs—known as short-range-correlated pairs—that shape the high-momentum structure of nuclear matter. Studying how nucleons form short-range-correlated pairs provides a rare experimental window into the short-distance behaviour of the strong interaction. Here, in this study, we use the scattering of high-energy electrons from 40 Ca, 48 Ca and 54 Fe, chosen for their distinct shell structures, to probe the formation of short-range-correlated pairs. Unexpectedly, we find that short-range-correlated pairing depends far more on the specific quantum orbitals occupied by protons and neutrons than on the nuclear mass or neutron–proton imbalance. This dependence is much stronger than that predicted by theoretical models. Our results point to a need for new angular-momentum quantum selection rules governing short-range nucleon pairing and reveal a deep connection between long-range nuclear shell structure and short-range interactions.

Nguyen, D. [Thomas Jefferson National Accelerator

JENSA: Past, present, and future

Nuclear reaction studies rely on three main physical components: the beam of nuclei provided by the facility, the detector systems used to measure the outgoing particles of interest, and the target. Target fabrication is thus a critical aspect of studying the reactions that power stars and probe the evolution of nuclear structure. The Jet Experiments in Nuclear Structure and Astrophysics (JENSA) gas jet target is the most dense helium jet target for rare isotope beam reaction studies in the world, providing targets of gaseous elements such as helium, nitrogen, and neon. A brief overview of the design and operation of JENSA, including commissioning and recent science experiments, and a discussion the future of JENSA coupled to the dedicated recoil separator SECAR, are presented.

Chipps, Kelly [ORNL] (ORCID:0000000330501298)

Design of structures for Nuclear Electric Propulsion vehicles

This paper reports a study of efficient structures for connecting various elements of Nuclear Electric Propulsion (NEP) vehicles. The design requirements for the structure are discussed and a truss beam is selected for the application. Evaluation of stiffness and weight indicate that the required structure is less than 5 percent of the dry weight of the vehicle.

Hedgepeth, John M.

Shell structure in 32P

The nuclear structure of 32P was investigated by detecting multi-γ coincidences along with charged light ions following the bombardment of an 18O target with a 30-MeV 16O beam from the Florida State University accelerator facility using the Clarion2-Trinity array of Clover γ spectrometers and GAGG (Gd-Al-Ga-garnet) scintillators for charged particles. A number of new states up to 12 MeV excitation and spins up to 9+ were observed by their γ decay patterns. Spins and parities were assigned by comparing measured γ angular distributions with polarizations inferred from Compton-scattering asymmetries between the Ge crystals in the Clover spectrometers. The new level scheme of 32P compares well with shell model calculations using the FSU cross-shell interaction and reasonably well with those using the sdpf-m interaction. In particular, structures with higher spin arise from promotion of more nucleons up to the f-p shell.

Allmond, James [ORNL] (ORCID:0000000165338721)