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Nasa GeneLab Computomics Reveal Horizontal Gene Transfer on International Space Station Environmental Metagenomes

Prokaryotic lifeforms can be observed to demonstrate many keen adaptive advantages, perhaps facilitated by a nature simplistic relative to divergent domains of life. In particular, decompartmentalized gene expression facilitates adaptation by allowing free exchange of genetic material, albeit at the cost of increased susceptibility to genetic damage. Thus, these lifeforms must compensate by embracing diverse investment strategies in an attempt to “brute force” the evolvability equation through precipitous genesis, lean metabolic efficiency, and sheer population. This prokaryotic archetype also enables symbiotic relationships with secondary mobile genetic elements known as plasmids, which have been shown to drive evolution on rapid temporal scales through processes such as conjugation and transformation. This study attempts to decipher whether these mechanisms of horizontal gene transfer (HGT) are major factors in determining prokaryote fitness within a unique isolated environment, the International Space Station (ISS). The ISS Microbial Tracking (MT) project has generated a wealth of data concerning the successive reigns of microbial genera that appear to thrive amidst harsh conditions for life. Despite relatively higher doses of ionizing radiation as compared to Earth, complications associated with microgravity, and the anti-microbial mélange deployed, microbial life still persists in this environment. The NASA GeneLab serves as a data repository and analysis platform to enable researchers to access space flight factor related data. With the use of GeneLab’s modern computational suites (computomics), phylogenetic and functional genomic investigations of HGT events were conducted on the data generated from the MT-1 project. The putative data concerning the plasmid population (plasmidome) of the ISS was algorithmically derived and compared to those of habitats with similar environmental dynamics- such as living quarters and hospitals- to investigate whether these HGT elements may play crucial role(s) in shaping the microbiome of this closed habitat that serves as the only inhabited structure in space.

Bense, Nicholas

Superconducting gravity gradiometer and a test of inverse square law

The equivalence principle prohibits the distinction of gravity from acceleration by a local measurement. However, by making a differential measurement of acceleration over a baseline, platform accelerations can be cancelled and gravity gradients detected. In an in-line superconducting gravity gradiometer, this differencing is accomplished with two spring-mass accelerometers in which the proof masses are confined to motion in a single degree of freedom and are coupled together by superconducting circuits. Platform motions appear as common mode accelerations and are cancelled by adjusting the ratio of two persistent currents in the sensing circuit. The sensing circuit is connected to a commercial SQUID amplifier to sense changes in the persistent currents generated by differential accelerations, i.e., gravity gradients. A three-axis gravity gradiometer is formed by mounting six accelerometers on the faces of a precision cube, with the accelerometers on opposite faces of the cube forming one of three in-line gradiometers. A dedicated satellite mission for mapping the earth's gravity field is an important one. Additional scientific goals are a test of the inverse square law to a part in 10(exp 10) at 100 km, and a test of the Lense-Thirring effect by detecting the relativistic gravity magnetic terms in the gravity gradient tensor for the earth.

Moody, M. V.

Co-transplantation of plasmid-transfected myoblasts and myotubes into rat brains enables high levels of gene expression long-term

We have previously proposed the use of primary muscle cells as a "platform," or "vehicle" for intracerebral transgene expression. Brain grafts of minced muscle, or cultured muscle cells persisted in rat brains for at least 6 mo without any decrease in graft size, or tumor formation. Stable, but moderate levels of intracerebral transgene expression were obtained by transplanting plasmid-transfected myotubes in culture. In the present study, high and stable levels of intracerebral transgene expression were achieved by the co-transplantation of plasmid-transfected myoblasts and myotubes in culture. Approximately 5 X 10(5) myoblasts and myotubes were transfected with 10 micrograms pRSVL plasmid DNA, and 30 micrograms Lipofectin (BRL), respectively. They were mixed together (total cell number was 1 million), and stereotactically injected into the caudate nucleus of an adult rat brain. The activity of luciferase, the product of transgene expression, was stable for at least 4 mo, and much higher than the levels in myotube grafts, or co-grafts of myoblasts and minced muscle. Presumably, the myotubes served as a framework on which the myoblasts can form myotubes. The sections of brains transplanted with co-graft of myoblasts, and myotubes transfected with pRSVLac-Z were stained immunofluorescently for beta-galactosidase activity. The muscle grafts contained beta-galactosidase positive myofibers 4 mo after transplantation. Such high and stable levels of in vivo expression after postnatal gene transfer have rarely been achieved. Primary muscle cells are useful vehicle for transgene expression in brains, and potentially valuable for gene therapy of degenerative neurological disorders.

NASA Program Space Biology

System and Method for Providing a Climate Data Persistence Service

A system, method and computer-readable storage devices for providing a climate data persistence service. A system configured to provide the service can include a climate data server that performs data and metadata storage and management functions for climate data objects, a compute-storage platform that provides the resources needed to support a climate data server, provisioning software that allows climate data server instances to be deployed as virtual climate data servers in a cloud computing environment, and a service interface, wherein persistence service capabilities are invoked by software applications running on a client device. The climate data objects can be in various formats, such as International Organization for Standards (ISO) Open Archival Information System (OAIS) Reference Model Submission Information Packages, Archive Information Packages, and Dissemination Information Packages. The climate data server can enable scalable, federated storage, management, discovery, and access, and can be tailored for particular use cases.

Schnase, John L.

Mapping continental-scale biomass burning and smoke palls from the space shuttle

Space shuttle photographs have been used to map the areal extent of Amazonian smoke palls associated with biomass burning. Areas covered with smoke have increased from approximately 300,000 sq km to continental-size smoke palls of approximately 3,000,000 sq km. The smoke palls interpreted from the STS-48 data indicate that this phenomenon is persistent. Astronaut observations of such dynamic and vital environmental phenomena indicate the possibility of intergrating the earth observation capabilities of all space platforms in future modeling of the earth's dynamic processes.

Lulla, Kamlesh

A13H-2537: Above Cloud Aerosol Optical Properties in the South East Atlantic from airborne observations during ORACLES

Aerosol overlying marine stratocumulus clouds is a persistent feature in the southeast Atlantic during the biomass burning season, July through November. We sampled aerosol and cloud from an airborne research platform, the NASA P3, during the deployments of ObseRvations of CLouds above Aerosols and their intEractionS (ORACLES) encompassing the peak biomass burning season. We used the Spectrometer for Sky-Scanning, Sun-Tracking Atmospheric Research (4STAR) to quantify the aerosol’s optical properties, notably the aerosol optical depth (AOD) by directly measuring the amount of light attenuation. We present an overview of very recent AOD measurements and contrast those to previous deployments. We observed an average AOD at 500 nm of 0.33 (0.30) in September 2016 (August 2017), with similar spatial dependence as observed by the MODIS instrument on board the TERRA and AQUA satellites. We also show full spectral vertical dependence of AOD from flight profiles, which compares favorably to the integrated aerosol extinction profile obtained by the active remote sensing High Spectral Resolution Lidar-2 (HSRL-2). We also present a quantification of the gap extent separating cloud top and aerosol layer bottom. Using hyperspectral transmitted light measurements from 4STAR, in conjunction with hyperspectral hemispheric irradiance measurements from the Solar Spectral Flux Radiometers (SSFR), we retrieved aerosol intensive properties (asymmetry parameter, single scattering albedo), aerosol size distributions, cloud optical depth (COD), cloud particle effective radius, and cloud thermodynamic phase. Cloud properties, also presented here, are obtained from 4STAR measurements of scattered light below clouds.

Samuel E LeBlanc

An Overview of the NASA Spring/Summer 2008 Arctic Campaign - ARCTAS (Arctic Research of the Composition of the Troposphere from Aircraft and Satellites)

ARCTAS (Arctic Research of the Composition of the Troposphere from Aircraft and Satellites) is a major NASA led airborne field campaign being performed in the spring and summer of 2008 at high latitudes (http://cloud1.arc.nasa.gov/arctas/). ARCTAS is a part of the International Polar Year program and its activities are closely coordinated with multiple U. S. (NOAA, DOE), Canadian, and European partners. Observational data from an ensemble of aircraft, surface, and satellite sensors are closely integrated with models of atmospheric chemistry and transport in this experiment. Principal NASA airborne platforms include a DC-8 for detailed atmospheric composition studies, a P-3 that focuses on aerosols and radiation, and a B-200 that is dedicated to remote sensing of aerosols. Satellite validation is a central activity in all these platforms and is mainly focused on CALIPSO, Aura, and Aqua satellites. Major ARCTAS themes are: (1) Long-range transport of pollution to the Arctic including arctic haze, tropospheric ozone, and persistent pollutants such as mercury; (2) Boreal forest fires and their implications for atmospheric composition and climate; (3) Aerosol radiative forcing from arctic haze, boreal fires, surface-deposited black carbon, and other perturbations; and (4) Chemical processes with focus on ozone, aerosols, mercury, and halogens. The spring deployment (April) is presently underway and is targeting plumes of anthropogenic and biomass burning pollution and dust from Asia and North America, arctic haze, stratosphere-troposphere exchange, and ozone photochemistry involving HOx and halogen radicals. The summer deployment (July) will target boreal forest fires and summertime photochemistry. The ARCTAS mission is providing a critical link to enhance the value of NASA satellite observations for Earth science. In this talk we will discuss the implementation of this campaign and some preliminary results.

Jacob, Daniel J.

Lighter-Than-Air (LTA) "AirStation": Unmanned Aircraft System (UAS) Carrier Concept

Proposals for adapting modern airship technologies for military missions have mostly focused on exploiting the airships high flight endurance and low fuel requirement to conduct direct surveillance missions requiring high degrees of persistence over the areas to be observed. While this mission has value, it constrains the airship in two regards. (1) It places all the surveillance sensors, communication systems, and other mission equipment in the airship itself. (2) It requires the airship to be physically in the vicinity of the areas to be directly observed. A more advanced utilization of airship technology would be to add the capability to deploy a separate set of surveillance equipment, thereby enabling indirect and distributed observation operations. This can be undertaken by installing surveillance equipment in a squadron of unmanned aircraft systems (UAS) that can be carried and operated remotely from the airship, and then return to the airship as a base of support. This could be accomplished by deploying 20-30 UASs on an optionally manned (5 person crew) airship. The mission focus of the airship UAS carrier would be for support of distributed intelligence, surveillance and reconnaissance (ISR), close air support (CAS), maritime patrol and interdiction, electronic warfare (EW), persistent area dominance and missile defense. The logic for utilizing an airship carrier over a ground base to deploy UAS will be examined. Whether to be used as a stand-alone platform or in concert with conventional intelligence gathering techniques, the airship UAS carrier can provide the following benefits: a mobile base that will remain accessible despite political fallout which may render a ground base unavailable for use, the psychological impact of a power projection tool that has no geographical limits (imagined in the same way a naval carrier group projects power), cost-saving intelligence gathering over manned alternatives (assumption), and a wider area of influence when compared to an immobile ground base that must facilitate the transfer of UAS to other bases in order to overfly particular areas (all operations, launch, recover, etc. take place from the airship).

Hochstetler, Ron

Satellite Snow-Cover Mapping: A Brief Review

Satellite snow mapping has been accomplished since 1966, initially using data from the reflective part of the electromagnetic spectrum, and now also employing data from the microwave part of the spectrum. Visible and near-infrared sensors can provide excellent spatial resolution from space enabling detailed snow mapping. When digital elevation models are also used, snow mapping can provide realistic measurements of snow extent even in mountainous areas. Passive-microwave satellite data permit global snow cover to be mapped on a near-daily basis and estimates of snow depth to be made, but with relatively poor spatial resolution (approximately 25 km). Dense forest cover limits both techniques and optical remote sensing is limited further by cloudcover conditions. Satellite remote sensing of snow cover with imaging radars is still in the early stages of research, but shows promise at least for mapping wet or melting snow using C-band (5.3 GHz) synthetic aperture radar (SAR) data. Observing System (EOS) Moderate Resolution Imaging Spectroradiometer (MODIS) data beginning with the launch of the first EOS platform in 1998. Digital maps will be produced that will provide daily, and maximum weekly global snow, sea ice and lake ice cover at 1-km spatial resolution. Statistics will be generated on the extent and persistence of snow or ice cover in each pixel for each weekly map, cloudcover permitting. It will also be possible to generate snow- and ice-cover maps using MODIS data at 250- and 500-m resolution, and to study and map snow and ice characteristics such as albedo. been under development. Passive-microwave data offer the potential for determining not only snow cover, but snow water equivalent, depth and wetness under all sky conditions. A number of algorithms have been developed to utilize passive-microwave brightness temperatures to provide information on snow cover and water equivalent. The variability of vegetative Algorithms are being developed to map global snow and ice cover using Earth Algorithms to map global snow cover using passive-microwave data have also cover and of snow grain size, globally, limits the utility of a single algorithm to map global snow cover.

Hall, Dorothy K.

Spatially Complete Global Surface Albedos Derived from Terra/MODIS Data

Spectral land surface albedo is an important parameter for describing the radiative properties of the Earth. Accordingly it reflects the consequences of natural and human interactions, such as anthropogenic, meteorological, and phenological effects, on global and local climatological trends. Consequently, albedos are integral parts in a variety of research areas, such as general circulation models (GCMs), energy balance studies, modeling of land use and land use change, and biophysical, oceanographic, and meteorological studies. , Over five years of land surface anisotropy, diffuse bihemispherical (white-sky) albedo and direct beam directional hemispherical (black-sky) albedo from observations acquired by the MODIS instruments aboard NASA s Terra and Aqua satellite platforms have provided researchers with unprecedented spatial, spectral, and temporal information on the land surface s radiative characteristics. However, roughly 30% of the global land surface, on an annual equal-angle basis, is obscured due to persistent and transient cloud cover, while another 207% is obscured due to ephemeral and seasonal snow effects. This precludes the MOD43B3 albedo products from being directly used in some remote sensing and ground-based applications, climate models, and global change research projects. To provide researchers with the requisite spatially complete global snow-free land surface albedo dataset, an ecosystem-dependent temporal interpolation technique was developed to fill missing or lower quality data and snow covered values from the official MOD43B3 dataset with geophysically realistic values. The method imposes pixel-level and local regional ecosystem-dependent phenological behavior onto retrieved pixel temporal data in such a way as to maintain pixel-level spatial and spectral detail and integrity. The phenological curves are derived from statistics based on the MODIS MOD12Q1 IGBP land cover classification product geolocated with the MOD43B3 data.

King, Michael D.

A study of persistent elevated pollution episodes in the northeastern United States

To examine chemical transformation within stagnant air masses and the atmospheric processes acting upon such air masses, the United States EPA sponsored a study in the summer of 1980 in the northeastern region of the country. Ten research aircraft and several mobile and stationary surface monitoring platforms participated in an intensive measurement program between 16 July and 15 August 1980. Pollutants of interest included SO2, NO, NO(x), HC, O3, sulfate, nitrate, and aerosols in general. This paper summarizes the activities of these research aircraft. Three aircraft and one helicopter logged a total of 353 flight hours during 100 missions in the program. The flight patterns and data collected for each mission are documented. This summary will allow scientists who are interested in this data base to identify subsets of the data for model development.

Vaughan, W. M.

Validation of Multisystem Countermeasures Protocol for Spaceflight during Antarctica Winter-over at Palmer Station (Palmer Countermeasures)

Exploration-class missions beyond the Van Allen belt to the Moon and then Mars will begin soon. Low-Earth orbital spaceflight results in the persistent perturbation of the human immune system, characterized by reductions in T and NK cell function, altered cytokine profiles, and the reactivation of latent herpesviruses. While these alterations have not caused widespread clinical issues, some crewmembers experience immune-related adverse events, including manifestations of symptomatic herpes viral reactivation, allergy, and respiratory distress. Because future deep-space exploration missions will be of unprecedented duration, it is reasonable to hypothesize that the immune perturbations observed aboard International Space Station (ISS) will intensify during longer missions in deep space, thereby placing crewmembers at elevated clinical risk. Thus, it is imperative to preserve the immune vigilance of astronauts by developing a countermeasure strategy. Of all the Earth analogs studied to date, an Antarctica winter-over (AWO) mission most closely reproduces the spaceflight experience: prolonged deployment, extreme environment, circadian misalignment, isolation, station lifestyle, and personal risk. The US maintains three primary stations in Antarctica: South Pole Station, McMurdo, and Palmer. Previous studies suggest that stations located near the interior of Antarctica (South Pole, McMurdo) have confounding effects on the immune system due to persistent hypobaric hypoxia. Thus, it was hypothesized that winter-over at a coastal station (Palmer) would be more akin to spaceflight due to its normoxic but still extreme environment. Therefore, AWO at Palmer Station was chosen as the platform for testing and validating the effectiveness of a NASA multi-system countermeasures protocol designed for deep space missions. The array of countermeasure protocols and monitoring methods deployed for each AWO will consist of diet modifications, nutritional supplementation, prescribed aerobic and resistive exercise, and a protocol of stress relieving virtual reality exercises. A multitude of biological sample types, including blood, saliva, and hair will be collected in tandem with the countermeasures in order to examine the combined effectiveness of the countermeasures. Samples and logs from subjects will be transported from Palmer Station to Johnson Space Center for further processing and distribution to co-investigators at the end of each winter-over. Extracted samples will be analyzed by appropriate testing platforms (Multiplex, qPCR, ELISA, etc.) to monitor alterations in leukocyte distribution, T cell and NK function, cytokine profiles, reactivation of latent herpesviruses, and nutritional factors. The data collected will be compared to a control year in which no countermeasures were deployed to evaluate the overall effectiveness of the analog and to validate the candidate immune countermeasure strategy. With the completion of the Antarctica Winter-Over (WO) 2022 control year, samples for 13 subjects have been successfully returned from Antarctica to NASA/JSC for further processing and distribution to Co-Investigators. WO 2023, the first countermeasure year, has also commenced with 11 subject consenting and performing their base line data collections (BDC) held in Chile. Another 5 subjects, who were already stationed at Palmer Station, Antarctica, joined as participates in the investigation. These 5 subjects were consented, but no BDC was able to be collected due to their joining in-mission. Therefore, there will be a total of 16 subjects participating in Antarctica's 2023 Winter-Over.

Cody L Gutierrez

Autonomous Medical Officer Support (AMOS) Software Technology Demonstrations on the International Space Station (ISS)

Performance of medical procedures in spaceflight beyond low Earth orbit (LEO) requires novel solutions to replace real-time ground support because as distance from Earth increases, communication latencies increase, hampering remote guidance. The Autonomous Medical Officer Support Software (AMOS) Technology Demonstrations on the International Space Station (ISS) trialed a novel software tool that shifts the emphasis from preflight training and real-time remote guidance (current ISS paradigm) to a new standard of multi-dimensional in-flight just-in-time (JIT) instruction. The AMOS platform is a skill management tool for all mission phases and currently features comprehensive training and guidance modules for urinary bladder and renal ultrasound examinations. Variability in Subject anatomy, Operator experience, and Operator receptiveness to instruction during autonomous exams are persistent but manageable limitations. Here we report the first successful demonstrations of autonomous imaging activities in the operational setting of spaceflight, validating this autonomous guidance proof of concept.

Douglas Ebert

Validation of Multisystem Countermeasures Protocol for Spaceflight during Antarctica Winter-over at Palmer Station (Palmer Countermeasures)

Stressors associated with spaceflight induce persistent immune compromise in astronauts which increase subclinical latent virus reactivation. In select crews, adverse clinical events have been documented. Antarctica winter-over (AWO) mission most closely reproduces these mission stressors: prolonged deployment, extreme environment, circadian misalignment, isolation, station lifestyle, and personal risk. The US maintains three primary stations in Antarctica: South Pole Station, McMurdo, and Palmer. Previous studies suggest that stations located near the interior of Antarctica (South Pole, McMurdo) have confounding effects on the immune system due to persistent hypobaric hypoxia. We hypothesized that winter-over at a coastal station (Palmer) would be more akin to spaceflight due to its normoxic but still extreme environment. Therefore, AWO at Palmer Station was selected, and validated in a pilot study, as the platform for testing and validating the effectiveness of an immune-restorative countermeasure protocol designed for deep space missions. Specifics include diet modifications, nutritional supplementation (vitamin D, probiotic, etc.), prescribed aerobic and resistive exercise, and a protocol of stress relieving virtual reality exercises. A multitude of biological sample types, including blood, saliva, and hair will be collected in tandem with the countermeasures in order to examine the combined effectiveness of the countermeasures. Samples and logs from subjects will be transported from Palmer Station to Johnson Space Center for further processing and distribution to co-investigators at the end of each winter-over. Extracted samples will be analyzed by appropriate testing platforms (Multiplex, qPCR, ELISA, etc.) to monitor alterations in leukocyte distribution, T cell and NK function, cytokine profiles, reactivation of latent herpesviruses, and nutritional factors. The data collected will be compared to a control year in which no countermeasures were deployed to evaluate the overall effectiveness of the analog and to validate the candidate immune countermeasure strategy. AWO 2023 concluded with the 4th in-mission timepoint conducted in September 2023. Samples for 16 subjects, including blood, saliva, hair, surveys, and PCR data, were all successfully returned from Antarctica to NASA/JSC mid-November 2023. Samples have since been distributed to co-investigators for further processing and analysis. With the completion of the first countermeasure year, preliminary data on the effectiveness of the deep-space protocol is being evaluated, however, no conclusions can be drawn yet until the completion of the second AWO countermeasure year, AWO 2024. AWO 2024 commenced in late-March 2024, with 13 subjects consenting and performing their baseline data collections (BDCs). Unique to the 2024 deployment, NSF lifted certain COVID restrictions and rallied the crewmembers in Punta Arenas, Chile. All NSF activities were transferred to this location and NASA was allowed, for the first time, to perform consent briefings, baseline samplings and training in person. This augment greatly increased the likelihood of success for the overwinter activities.

Cody L Gutierrez

Validation of Multisystem Countermeasures Protocol for Spaceflight during Antarctica Winter-over at Palmer Station (Palmer Countermeasures)

Stressors associated with spaceflight induce persistent immune compromise in astronauts which increase subclinical latent virus reactivation. In select crews, adverse clinical events have been documented. Antarctica winter-over (AWO) mission most closely reproduces these mission stressors: prolonged deployment, extreme environment, circadian misalignment, isolation, station lifestyle, and personal risk. The US maintains three primary stations in Antarctica: South Pole Station, McMurdo, and Palmer. Previous studies suggest that stations located near the interior of Antarctica (South Pole, McMurdo) have confounding effects on the immune system due to persistent hypobaric hypoxia. We hypothesized that winter-over at a coastal station (Palmer) would be more akin to spaceflight due to its normoxic but still extreme environment. Therefore, AWO at Palmer Station was selected, and validated in a pilot study [2], as the platform for testing and validating the effectiveness of an immune-restorative countermeasure protocol designed for deep space missions. Specifics include diet modifications, nutritional supplementation (vitamin D, probiotic, etc.), prescribed aerobic and resistive exercise, and a protocol of stress relieving virtual reality exercises. A multitude of biological sample types, including blood, saliva, and hair will be collected in tandem with the countermeasures in order to examine the combined effectiveness of the countermeasures. Samples and logs from subjects will be transported from Palmer Station to Johnson Space Center for further processing and distribution to co-investigators at the end of each winter-over. Extracted samples will be analyzed by appropriate testing platforms (Multiplex, qPCR, ELISA, etc.) to monitor alterations in leukocyte distribution, T cell and NK function, cytokine profiles, reactivation of latent herpesviruses, and nutritional factors. The data collected will be compared to a control year in which no countermeasures were deployed to evaluate the overall effectiveness of the analog and to validate the candidate immune countermeasure strategy. AWO 2023 concluded with the 4th in-mission timepoint conducted in September 2023. Samples for 16 subjects, including blood, saliva, hair, surveys, and PCR data, were all successfully returned from Antarctica to NASA/JSC mid-November 2023. Samples have since been distributed to co-investigators for further processing and analysis. With the completion of the first countermeasure year, preliminary data on the effectiveness of the deep-space protocol is being evaluated, however, no conclusions can be drawn yet until the completion of the second AWO countermeasure year, AWO 2024. AWO 2024 commenced in late-March 2024, with 13 subjects consenting and performing their baseline data collections (BDCs). Unique to the 2024 deployment, NSF lifted certain COVID restrictions and rallied the crewmembers in Punta Arenas, Chile. All NSF activities were transferred to this location and NASA was allowed, for the first time, to perform consent briefings, baseline samplings and training in person. This augment greatly increased the likelihood of success for the overwinter activities.

Cody L Gutierrez

Work Coordination Engine

The Work Coordination Engine (WCE) is a Java application integrated into the Service Management Database (SMDB), which coordinates the dispatching and monitoring of a work order system. WCE de-queues work orders from SMDB and orchestrates the dispatching of work to a registered set of software worker applications distributed over a set of local, or remote, heterogeneous computing systems. WCE monitors the execution of work orders once dispatched, and accepts the results of the work order by storing to the SMDB persistent store. The software leverages the use of a relational database, Java Messaging System (JMS), and Web Services using Simple Object Access Protocol (SOAP) technologies to implement an efficient work-order dispatching mechanism capable of coordinating the work of multiple computer servers on various platforms working concurrently on different, or similar, types of data or algorithmic processing. Existing (legacy) applications can be wrapped with a proxy object so that no changes to the application are needed to make them available for integration into the work order system as "workers." WCE automatically reschedules work orders that fail to be executed by one server to a different server if available. From initiation to completion, the system manages the execution state of work orders and workers via a well-defined set of events, states, and actions. It allows for configurable work-order execution timeouts by work-order type. This innovation eliminates a current processing bottleneck by providing a highly scalable, distributed work-order system used to quickly generate products needed by the Deep Space Network (DSN) to support space flight operations. WCE is driven by asynchronous messages delivered via JMS indicating the availability of new work or workers. It runs completely unattended in support of the lights-out operations concept in the DSN.

Zendejas, Silvino

Merging Analytic Collaborative Frameworks with New Observing Strategies Toward a Digital Twin: Earth – Episodic Pulse Event Impacts on Ocean Carbon Cycle as an Example

Virtual representations of the Earth will allow us to address some of the most critical environmental issues of our time. Here, we show the first steps toward representation of riverine, estuarine, and coastal carbon processes to enable scenario driven “what-if” analyses of the carbon system and human footprint. Excess sediment and nutrient runoff from land-based human activities impact water quality and can pose serious threats to coastal and marine ecosystems. Episodic pulse events, such as extreme precipitation events, can increase the amount of nutrients entering estuaries and coastal regions, potentially leading to large phytoplankton blooms followed by anoxic conditions. Consequences of coastal runoff are predicted to increase with the higher intensity and frequency of extreme events. Beyond the threat to coastal ecosystems, recent findings suggest these episodic pulses might play a significant role for biological production influencing regional and global carbon fluxes and budgets. An improved understanding of these events through optimal, dynamic observing strategies will increase our knowledge of the land-ocean continuum and how regional events and nutrient fluxes affect the carbon cycle and ocean ecosystem. This conceptual framework enables focused science investigations by pairing data analytics and artificial intelligence tools (otherwise termed an Analytic Center Framework, ACF) with targeted measurement acquisition through distributed sensing and intelligent asset tasking (or New Observing Strategies, NOS). This NOS and ACF iterative approach acquires and integrates complementary and coincident satellite, in-situ and model data to build a more complete and in-depth picture of science phenomena. Specifically, Apache Science Data Analytic Platform (SDAP) is extended to incorporate relevant datasets for data access, harmonized analysis, and anomaly detection. When conditions are met for a likely pulse event, NASA’s D-SHIELD (Distributed Spacecraft with Heuristic Intelligence to Enable Logistical Decisions) tool is triggered to optimize asset overpass frequency and schedule observations for persistent monitoring. Targeted data is ingested by SDAP for enhanced investigation via iterative analysis until the trigger criteria is no longer met - steps toward a digital twin.

Laura Rogers

How Does Mediterranean Basin's Atmosphere Become Weak Moisture Source During Negative Phase of NAO: Use of AIRS, AMSR, TOVS, & TRMM Satellite Datasets Over Last Two NAO Cycles to Examine Governing Controls on E-P

The Mediterranean Sea is a noted 'concentration" basin in that it almost continuously exhibits positive evaporation minus precipitation (E - P ) properties -- throughout the four seasons and from one year to the next. Nonetheless, according to the ECMWF Era-40 48-year (1958-2005) climate reanalysis dataset, for various phases of the North Atlantic Oscillation (NAO) when the pressure gradient between Portugal and Iceland becomes either very relaxed (large negative NAO-Index) or in transition (small positive or negative NAO-Index), the atmospheric moisture source properties of the basin become weak, at times even reversed for several months (i.e., negative E - P). This behavior poses numerous questions concerning how and why these events occur. Moreover, it begs the question of what it would take for the basin to reach its tipping point in which P would exceed E throughout the rainy season (some six months) on an annually persistent basis -- and the sea would possibly transform to a recurring "dilution" basin. This talk investigates these questions by: (1) establishing over a period from 1979 to present, based on detailed analyses of satellite retrieval products from a combination of NASA-AQUA, NOAA-LEO, NASA/JAXA Scatterometer, and NASA-TRMM platforms, plus additional specialized satellite data products and ancillary meteorological datasets, the actual observation-based behavior of E - P, (2) diagnosing the salient physical and meteorological mechanisms that lead to the weaker E - P events during the analysis period, partly based on analyzing surface and upper air data at discrete stations in the western and eastern Mediterranean -- while at the same time evaluating the quality of the ERA-40 data over this same time period, (3) conducting GCM and high-resolution regional modeling experiments to determine if perturbed but realistic meteorological background conditions could maintain Mediterranean as a "dilution" basin through the October to March rainy season on annually recurring basis, and (4) investigating how such conditions might modify important internal and external climatic processes known to be closely related to the dynamical, thermal, and hydrological properties of the basin (e.g., drought frequency over Iberian peninsula, rainfall accumulation within Sahel, alteration of Levantine branch of east-west aligned open thermohaline cell, and modification of warm-salty intermediate flow through Gibraltar straight into North Atlantic).

Smith, Eric A.