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Human life support for advanced space exploration

The requirements for a human life support system for long-duration space missions are reviewed. The system design of a controlled ecological life support system is briefly described, followed by a more detailed account of the study of the conceptual design of a Lunar Based CELSS. The latter is to provide a safe, reliable, recycling lunar base life support system based on a hybrid physicochemical/biological representative technology. The most important conclusion reached by this study is that implementation of a completely recycling CELSS approach for a lunar base is not only feasible, but eminently practical. On a cumulative launch mass basis, a 4-person Lunar Base CELSS would pay for itself in approximately 2.6 years relative to a physicochemical air/water recycling system with resupply of food from the Earth. For crew sizes of 30 and 100, the breakeven point would come even sooner, after 2.1 and 1.7 years, respectively, due to the increased mass savings that can be realized with the larger plant growth units. Two other conclusions are particularly important with regard to the orientation of future research and technology development. First, the mass estimates of the Lunar Base CELSS indicate that a primary design objective in implementing this kind of system must be to minimized the mass and power requirement of the food production plant growth units, which greatly surpass those of the other air and water recycling systems. Consequently, substantial research must be directed at identifying ways to produce food more efficiently. On the other hand, detailed studies to identify the best technology options for the other subsystems should not be expected to produce dramatic reductions in either mass or power requirement of a Lunar Base CELSS. The most crucial evaluation criterion must, therefore, be the capability for functional integration of these technologies into the ultimate design of the system. Secondly, this study illustrates that existing or near-term technologies are adequate to implement a Lunar Base CELSS. There are no apparent "show-stoppers" which require the development of new technologies. However, there are several areas in which new materials and technologies could be used for a more efficient implementation of the system, e.g., by decreasing mass or power requirement and increasing recycling efficiency. These areas must be further addressed through research and development. Finally, although this study focused on the development of a Lunar Base CELSS, the same technologies and a nearly identical design would be appropriate for a Mars base. Actually, except for the distance of transportation, the implementation of a CELSS on Mars would even be easier than it would be on the Moon. The presence of atmospheric CO2 on Mars, although in low concentration, coupled with the fact that the day/night cycle on Mars is very similar to that on Earth, makes the use of light-weight, greenhouse-like structures for growing food plants even more feasible than on the Moon. There are some environmental problems, which would have to be dealt with, like dust storms and the large amount of the ultraviolet radiation incident on the planet's surface. However, the materials and methods are largely available today to develop such a life support system for a Mars base.

Review, Tutorial↗

Weaving Together Space Biology and the Human Research Program: Selecting Crops and Manipulating Plant Physiology to Produce High Quality Food for ISS Astronauts

Researchers from the Human Research Program (HRP) have teamed up with plant biologists at KSC to explore the potential for plant growth and food production on the international space station (ISS) and future exploration missions. KSC Space Biology (SB) brings a history of plant and plant-microbial interaction research for station and for future bioregenerative life support systems. JSC HRP brings expertise in Advanced Food Technology (AFT), Advanced Environmental Health (AEH), and Behavioral Health and Performance (BHP). The Veggie plant growth hardware on the ISS is the platform that first drove these interactions. As we prepared for the VEG-01 validation test of Veggie, we engaged with BHP to explore questions that could be asked of the crew that would contribute both to plant and to behavioral health research. AFT, AEH and BHP stakeholders were engaged immediately after the return of the Veggie flight samples of space-grown lettuce, and this team worked with the JSC human medical offices to gain approvals for crew consumption of the lettuce on ISS. As we progressed with Veggie testing we began performing crop selection studies for Veggie that were initiated through AFT. These studies consisted of testing and down selecting leafy greens, dwarf tomatoes, and dwarf pepper crops based on characteristics of plant growth and nutritional levels evaluated at KSC, and organoleptic quality evaluated at JSCs Sensory Analysis lab. This work has led to a successful collaborative proposal to the International Life Sciences Research Announcement for a jointly funded HRP-SB investigation of the impacts of light quality and fertilizer on salad crop productivity, nutrition, and flavor in Veggie on the ISS. With this work, and potentially with other pending joint projects, we will continue the synergistic research that will advance the space biology knowledge base, help close gaps in the human research roadmap, and enable humans to venture out to Mars and beyond.

International Space Station↗

Plants, plant pathogens, and microgravity--a deadly trio

Plants grown in spaceflight conditions are more susceptible to colonization by plant pathogens. The underlying causes for this enhanced susceptibility are not known. Possibly the formation of structural barriers and the activation of plant defense response components are impaired in spaceflight conditions. Either condition would result from altered gene expression of the plant. Because of the tools available, past studies focused on a few physiological responses or biochemical pathways. With recent advances in genomics research, new tools, including microarray technologies, are available to examine the global impact of growth in the spacecraft on the plant's gene expression profile. In ground-based studies, we have developed cDNA subtraction libraries of rice that are enriched for genes induced during pathogen infection and the defense response. Arrays of these genes are being used to dissect plant defense response pathways in a model system involving wild-type rice plants and lesion mimic mutants. The lesion mimic mutants are ideal experimental tools because they erratically develop defense response-like lesions in the absence of pathogens. The gene expression profiles from these ground-based studies will provide the molecular basis for understanding the biochemical and physiological impacts of spaceflight on plant growth, development and disease defense responses. This, in turn, will allow the development of strategies to manage plant disease for life in the space environment.

NASA Discipline Plant Biology↗

Development of an Automated Seed Sowing and Induced Germination System for Space Flight Application

The successful utilization of higher plants in space flight is likely to require the effective transition of plants through all phases of growth and development. A particularly sensitive and critical stage in this cycle is seed germination. The present inflight capability to manipulate seed from a state of dormancy to germination and the performance of such activity under aseptic conditions is extremely limited. An Automated Sowing Mechanism (ASM) has been designed to address this area of science and technology. The self-contained system is readily compatible with the existing Shuttle middeck locker Plant Growth Unit (PGU) and planned Plant Growth Facility (PGF), presenting an opportunity to extend the experimental capability of these systems. The ASM design encompasses the controlled transition of seed from a dry to hydrated state utilizing solid media substrate as the source of water and nutrient support. System activation has been achieved with both photo and timing mechanisms. Controlled induced germination and development of various plant species has been achieved in ground-based trials. The system is presently being prepared for a KC-135 flight test.

Heyenga, A. G.↗

New Technologies for Enabling Food Production Beyond LEO

NASA has identified the need for robust and sustainable ‘Pick-and-Eat’ systems for supplementing crew diets with fresh leafy green crops in near-term LEO (Low Earth Orbit), cislunar, and lunar missions. Spaceflight plant growth systems have been primarily designed for conducting space biology studies, but these systems are not optimal for sustained food production. Improved water and nutrient delivery subsystems that do not use bulky and non-reusable media are needed for decreasing the mass of the food production system. Autonomous technologies for monitoring plant health and food safety are needed for ensuring that the food produced is suitable supplementing crew diets with fresh, nutritious salad crops. Improved plant imaging techniques used for high-throughput phenotyping can be leveraged for monitoring plant health. Near-real-time measurements of the microbial ecology of food production systems are needed for assessing food safety. Furthermore, newly identified plant species and cultivars with improved growth habits and contents of antioxidants, vitamins, and minerals when grown in spaceflight environmental conditions are needed. These improvements in food production technologies will enable the design of sustainable life support systems for manned exploration missions beyond Low Earth Orbit.

Monje Mejia, Oscar A.↗

Improving Sanitation and Health in Rural Alaska

In rural Alaskan communities personal health is threatened by energy costs and limited access to clean water, wastewater management, and adequate nutrition. Fuel-­‐based energy systems are significant factors in determining local accessibility to clean water, sanitation and food. Increasing fuel costs induce a scarcity of access and impact residents' health. The University of Alaska Fairbanks (UAF) School of Natural Resources and Agricultural Sciences (SNRAS), NASA's Ames Research Center, and USDA Agricultural Research Service (ARS) have joined forces to develop high‐efficiency, low­‐energy consuming techniques for water treatment and food production in rural circumpolar communities. Methods intended for exploration of space and establishment of settlements on the Moon or Mars will ultimately benefit Earth's communities in the circumpolar north. The initial phase of collaboration is completed. Researchers from NASA Ames Research Center and SNRAS, funded by the USDA­‐ARS, tested a simple, reliable, low-energy sewage treatment system to recycle wastewater for use in food production and other reuse options in communities. The system extracted up to 70% of the water from sewage and rejected up to 92% of ions in the sewage with no carryover of toxic effects. Biological testing showed that plant growth using recovered water in the nutrient solution was equivalent to that using high‐purity distilled water. With successful demonstration that the low energy consuming wastewater treatment system can provide safe water for communities and food production, the team is ready to move forward to a full‐scale production testbed. The SNRAS/NASA team (including Alaska students) will design a prototype to match water processing rates and food production to meet rural community sanitation needs and nutritional preferences. This system would be operated in Fairbanks at the University of Alaska through SNRAS. Long­‐term performance will be validated and operational needs of the system determined. The testbed will be a part of the university education and operator training program. The "Forgotten Alaska" has long awaited this technology to augment the traditional

Plant Growth↗

Flexible Microsensor Array for the Root Zone Monitoring of Porous Tube Plant Growth System

Control of oxygen and water in the root zone is vital to support plant growth in the microgravity environment. The ability to control these sometimes opposing parameters in the root zone is dependent upon the availability of sensors to detect these elements and provide feedback for control systems. In the present study we demonstrate the feasibility of using microsensor arrays on a flexible substrate for dissolved oxygen detection, and a 4-point impedance microprobe for surface wetness detection on the surface of a porous tube (PT) nutrient delivery system. The oxygen microsensor reported surface oxygen concentrations that correlated with the oxygen concentrations of the solution inside the PT when operated at positive pressures. At negative pressures the microsensor shows convergence to zero saturation (2.2 micro mol/L) values due to inadequate water film formation on porous tube surface. The 4-point microprobe is useful as a wetness detector as it provides a clear differentiation between dry and wet surfaces. The unique features of the dissolved oxygen microsensor array and 4-point microprobe include small and simple design, flexibility and multipoint sensing. The demonstrated technology is anticipated to provide low cost, and highly reliable sensor feedback monitoring plant growth nutrient delivery system in both terrestrial and microgravity environments.

Sathyan, Sandeep↗

Media Effects on Lettuce Growth in "Pillows" Designed for the VEGGIE Spaceflight Growth Chamber

VEGGIE is a prototype vegetable production unit for space designed by Orbital Technologies Corporation that is being developed to fly on the International Space Station. A modular plant rooting system "pillow" is being designed to support plant growth in VEGGIE under microgravity conditions. VEGGIE pillows are small self-contained packets of media with time-release fertilizer that can wick water passively from a root mat reservoir. Seeds are planted in pillows and the entire root system of a plant is contained as the crop develops, preventing loss into the spacecraft cabin. This study compared five media types and three lettuce cultivars in pillows growing in a VEGGIE analog environment.. Media consisted of a peat-based potting mix (Fafard #2,Conrad Fafard Inc., Agawam, MA), and a calcined clay, (arcillite, 1-2 mm sifted, Turface Proleague, Profile LLC, Buffalo Grove IL) as well as three different blends of the two, 70:30, 50:50, and 30:70. Lettuce cultivars tested were 'Sierra', a bi-colored French crisp Batavia lettuce, 'Outredgeous', a red romaine lettuce and 'Flandria', a green butter head variety. Plants were grown for 28 days, harvested, biometric data was obtained, and tissue mineral analysis was performed. For all cultivars, lettuce plants grown in the media blends were more productive than those in the individual media types. All cultivars showed bell-shaped curves in response to increases in arcillite / decreases in Fafard #2 for leaf area, fresh, and dry mass. Plants in 100% Fafard #2 and in 100% arcillite were stunted, but only those in higher levels of Fafard #2 (70% and 100%) had reduced shoot percent moisture, possibly indicating that mechanisms causing stunting differed. Variation in tissue nutrient content are consistent with this, with Mg and Mn highest in plants grown in 100% Fafard and decreasing as the concentration of arcillite increased. Color also varied with media, especially in the 'Sierra' lettuce, with plants grown in increasing levels of Fafard #2 being much more red and those in 100% arcillite almost completely green. The red-leaf cultivar 'Outredgeous' showed increasing chlorophyll (SPAD values) with increasing percentage of arcillite. In all cultivars tested it appears that a mixture of media types, usually 50:50 or 30:70 Fafard #2: arcillite, sustained healthier, more productive plants. Smaller, less productive plants in either of the individual media may indicate stress issues, however more work is needed to understand the reasons for this sub-optimal growth. This work was supported by NASA

Massa, Gioia↗

Discharge lamp technologies

This talk is an overview of discharge lamp technology commonly employed in general lighting, with emphasis on issues pertinent to lighting for plant growth. Since the audience is primarily from the plant growth community, and this begins the light source part of the program, we will start with a brief description of the discharge lamps. Challenges of economics and of thermal management make lamp efficiency a prime concern in controlled environment agriculture, so we will emphasize science considerations relating to discharge lamp efficiency. We will then look at the spectra and ratings of some representative lighting products, and conclude with a discussion of technological advances.

Dakin, James↗

Space Crop Production Gaps and Challenges

As astronauts venture farther from Earth, and stay for longer periods, the space food system will increase in importance. Crop production can supplement a pre-packaged space diet to provide nutrition and dietary variety for space crews. In future missions, bioregenerative approaches may be used to generate a larger percentage of the diet, as well as help to reduce life support system burdens and resupply from Earth. Plants may also provide behavioral health benefits to crew members living in the isolated, confined environment of a space habitat. A number of unique challenges exist for growth of plants in microgravity and on other reduced gravity surfaces like the moon and Mars. Testing plant growth inside the Veggie and Advanced Plant Habitat (APH) chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth, nutritional content, acceptability, and the importance of plants to astronauts living and working away from Earth. We are also gaining a better understanding of food safety concerns and the behavior of space plant microbiomes and plant pathogens, but major gaps in knowledge remain. As we move from research towards operational space crop production to enable exploration, there are numerous gaps in technology, knowledge, and practice related to space crop growth that must be addressed. Research and development in key focus areas such as effective water and nutrient delivery at variable gravity levels, autonomous plant health monitoring, growth system cleaning and disinfection, and selection of ideal space crops are needed to fill these gaps. Breeding or engineering custom space crops may impact areas including plant growth and development, plant physiology, produce nutrition, organoleptic acceptability, and post-harvest characteristics, and these may further enable space crop production scenarios. Space crop challenges are multifaceted and require diverse interdisciplinary teams working together to develop effective solutions. Solving these requires an array of skill sets from across the biological and physical sciences, engineering, and human social sciences. Solutions to help ensure food security off-Earth may also translate to more sustainable terrestrial crop production approaches, and regular dialog between industry, academia, and government organizations working in related fields benefit all. Additional help can come from engagement with student researchers at various levels through courses, participatory science projects, and open science activities which can provide useful data. Global coordination and integration between space agencies and partners will be essential.

Gioia Donna Massa↗

Space Crop Production Gaps and Challenges

As astronauts venture farther from Earth, and stay for longer periods, the space food system will increase in importance. Crop production can supplement a pre-packaged space diet to provide nutrition and dietary variety for space crews. In future missions, bioregenerative approaches may be used to generate a larger percentage of the diet, as well as help to reduce life support system burdens and resupply from Earth. Plants may also provide behavioral health benefits to crew members living in the isolated, confined environment of a space habitat. A number of unique challenges exist for growth of plants in microgravity and on other reduced gravity surfaces like the moon and Mars. Testing plant growth inside the Veggie and Advanced Plant Habitat (APH) chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth, nutritional content, acceptability, and the importance of plants to astronauts living and working away from Earth. We are also gaining a better understanding of food safety concerns and the behavior of space plant microbiomes and plant pathogens, but major gaps in knowledge remain. As we move from research towards operational space crop production to enable exploration, there are numerous gaps in technology, knowledge, and practice related to space crop growth that must be addressed. Research and development in key focus areas such as effective water and nutrient delivery at variable gravity levels, autonomous plant health monitoring, growth system cleaning and disinfection, and selection of ideal space crops are needed to fill these gaps. Breeding or engineering custom space crops may impact areas including plant growth and development, plant physiology, produce nutrition, organoleptic acceptability, and post-harvest characteristics, and these may further enable space crop production scenarios. Space crop challenges are multifaceted and require diverse interdisciplinary teams working together to develop effective solutions. Solving these requires an array of skill sets from across the biological and physical sciences, engineering, and human social sciences. Solutions to help ensure food security off-Earth may also translate to more sustainable terrestrial crop production approaches, and regular dialog between industry, academia, and government organizations working in related fields benefit all. Additional help can come from engagement with student researchers at various levels through courses, participatory science projects, and open science activities which can provide useful data. Global coordination and integration between space agencies and partners will be essential.

Gioia Massa↗

Hydroponics for Food Production in Space: History and Current Efforts

Goals of Food Production in Space: NASA Human Research Program (HRP) near-term food production systems - supplement key nutrients (Vitamins B1,C, K and potassium) that degrade to inadequate levels on exploration class missions; Study behavioral health aspect - caring for plants and improving the acceptability of the astronaut diet by supplementing it with fresh produce (currently anecdotal); Develop sustainable water delivery technologies - current TRL (Technological Readiness Level) 9 particulate based systems (i.e. used in research conducted in plant growth facilities APH (Advanced Plant Habitat), Veggie, BPS (Biomass Production System), LADA/SVET (Russian space plant chamber systems)) require resupply of bulky consumables and generate waste media.

Dreschel, T.↗

Plant Water Management (PWM)

The NASA Plant Water Management technology demonstration exploits recent advances in microgravity capillary fluidics to develop techniques for plant growth in a space environment. Plant growth in the low-g environment is often hampered by inadequate aeration and over-saturation in the root zone. The present effort aims to make passive use of poorly wetting liquids (i.e., contaminated water) within unique system geometries that effectively replace the role of gravity in providing sufficient aeration and hydration for simulated plants. Several ISS demonstrations are in preparation for launch in 2019, including hydroponic and soil approaches. Supportive terrestrial and low-g drop tower tests are conducted to aid in experiment design via proof-of-concepts, limits of operation, system stability, and others. The implications to short and long term applications are discussed in relation to plant growth facilities for both near-term microgravity plant science research and long duration human exploration missions.

Hatch, Tyler↗

Progressive Plant Growing Has Business Blooming

In 1997, AgriHouse, Inc. (d.b.a. Aeroponics International), a leading agri-biology company, united with NASA and BioServe Space Technologies, a nonprofit, NASA-sponsored partnership research center, to design a soil-less plant-growth experiment to be performed in microgravity, aboard the Mir space station. This experiment aimed to gauge the effectiveness of a non-pesticide solution on the immune responses of bean plants. In essence, the research consortium was looking for a means of keeping plants free from infection, without having to rely on the use of pesticides. This research, combined with follow-on grants from NASA, has helped Berthoud, Colorado-based AgriHouse gain credibility in the commercial marketplace with related technology and gross the capital necessary to conduct further research in a new-age field known as bio-pharming.

Source record↗

Light-emitting diodes as an illumination source for plants: a review of research at Kennedy Space Center

The provision of sufficient light is a fundamental requirement to support long-term plant growth in space. Several types of electric lamps have been tested to provide radiant energy for plants in this regard, including fluorescent, high-pressure sodium, and metal halide lamps. These lamps vary in terms of spectral quality, which can result in differences in plant growth and morphology. Current lighting research for space-based plant culture is focused on innovative lighting technologies that demonstrate high electrical efficiency and reduced mass and volume. Among the lighting technologies considered for space are light-emitting diodes (LEDs). The combination of red and blue LEDs has proven to be an effective lighting source for several crops, yet the appearance of plants under red and blue lighting is purplish gray, making visual assessment of plant health difficult. Additional green light would make the plant leaves appear green and normal, similar to a natural setting under white light, and may also offer psychological benefits for the crew. The addition of 24% green light (500-600 nm) to red and blue LEDs enhanced the growth of lettuce plants compared with plants grown under cool white fluorescent lamps. Coincidentally, these plants grown under additional green light would have the additional aesthetic appeal of a green appearance.

NASA Discipline Life Support Systems↗

Sustainable Crop Cultivation in Space Analogs: A BRIDGES Methodology Perspective Through SpaCEA

Sustainable crop cultivation in space holds paramount significance for the support of life in future long-duration missions. This research explores the development and integration of innovative low-cost proof-of-concept (LC-POC) plant growth cabinets tailored for use in space analog missions. By outlining past and current efforts in space farming, this study introduces the SpaCEA Cabinet using BRIDGES framework, establishing a context for reproducible experiments and innovation in plant growth systems. The SpaCEA cabinets can either be delivered in flat packs or assembled on-site, employing cutting-edge methods like 3-D printing and laser cutting. The main objective is to assess how effectively these structures foster crop growth within analog environments while replicating conditions crucial for space exploration. Employing a multi-faceted approach encompassing technical and qualitative dimensions, this project integrates a qualitative investigation where representatives managing analog stations and analog astronauts will partake in interviews and questionnaires to discern specific requirements and challenges within these environments. Insights gained from these engagements will significantly define the final design parameters of updated SpaCEA plant growth cabinets. The practical applicability of these cabinets emphasizes ease of assembly and transportation, addressing the inherent spatial and logistical constraints associated with space missions. Furthermore, the BRIDGES framework ensures the standardization of hardware, software, and data-gathering elements within a unified structure, which utilizes cutting-edge manufacturing technologies for the prototyping and deployment of these cabinets. The anticipated outcomes of this research include the identification of key design considerations and technical specifications for plant growth cabinets tailored to space farming analog systems. This research is poised to contribute valuable knowledge to sustainable space exploration through the development of interoperable plant growth systems for analog environments, advancing research in space crop cultivation which will make up part of a larger bioregenerative life support system.

Advanced Life Support↗

Sustainable Crop Cultivation in Space Analogs: A BRIDGES Methodology Perspective Through SpaCEA

Sustainable crop cultivation in space holds paramount significance for the support of life in future long-duration missions. This research explores the development and integration of innovative low-cost proof-of-concept (LC-POC) plant growth cabinets tailored for use in space analog missions. By outlining past and current efforts in space farming, this study introduces the SpaCEA Cabinet using BRIDGES framework, establishing a context for reproducible experiments and innovation in plant growth systems. The SpaCEA cabinets can either be delivered in flat packs or assembled on-site, employing cutting-edge methods like 3-D printing and laser cutting. The main objective is to assess how effectively these structures foster crop growth within analog environments while replicating conditions crucial for space exploration. Employing a multi-faceted approach encompassing technical and qualitative dimensions, this project integrates a qualitative investigation where representatives managing analog stations and analog astronauts will partake in interviews and questionnaires to discern specific requirements and challenges within these environments. Insights gained from these engagements will significantly define the final design parameters of updated SpaCEA plant growth cabinets. The practical applicability of these cabinets emphasizes ease of assembly and transportation, addressing the inherent spatial and logistical constraints associated with space missions. Furthermore, the BRIDGES framework ensures the standardization of hardware, software, and data-gathering elements within a unified structure, which utilizes cutting-edge manufacturing technologies for the prototyping and deployment of these cabinets. The anticipated outcomes of this research include the identification of key design considerations and technical specifications for plant growth cabinets tailored to space farming analog systems. This research is poised to contribute valuable knowledge to sustainable space exploration through the development of interoperable plant growth systems for analog environments, advancing research in space crop cultivation which will make up part of a larger bioregenerative life support system.

Advanced Life Support↗

Plant Water Management (PWM) Experiment

Plant Water Management is a technology demonstration of recent advances in micro-g capillary fluidics research applied to plant growth systems. It has applications in long-term food production systems for missions to the Moon and Mars, as well as the immediate need for ISS food supplements to the crew diet. PWM will demonstrate the low-gravity role of surface tension, wetting, and system geometry to effectively replace the role of gravity in certain terrestrial plant growth systems.

Hatch, Tyler↗