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At least 55 records · Page 3

Antisense expression of an Arabidopsis ran binding protein renders transgenic roots hypersensitive to auxin and alters auxin-induced root growth and development by arresting mitotic progress

We cloned a cDNA encoding an Arabidopsis Ran binding protein, AtRanBP1c, and generated transgenic Arabidopsis expressing the antisense strand of the AtRanBP1c gene to understand the in vivo functions of the Ran/RanBP signal pathway. The transgenic plants showed enhanced primary root growth but suppressed growth of lateral roots. Auxin significantly increased lateral root initiation and inhibited primary root growth in the transformants at 10 pM, several orders of magnitude lower than required to induce these responses in wild-type roots. This induction was followed by a blockage of mitosis in both newly emerged lateral roots and in the primary root, ultimately resulting in the selective death of cells in the tips of both lateral and primary roots. Given the established role of Ran binding proteins in the transport of proteins into the nucleus, these findings are consistent with a model in which AtRanBP1c plays a key role in the nuclear delivery of proteins that suppress auxin action and that regulate mitotic progress in root tips.

Non-NASA Center↗

A robust spectral element implementation of the $k - τ$ RANS model in Nek5000/NekRS

The $k - ω$ Reynolds Averaged Navier Stokes (RANS) model is one of the industry standard approaches for modeling of turbulent flows. It performs better than the $k - ϵ$ model for low Reynolds number flows and is also more suitable for boundary layers with adverse pressure gradients. Major drawback of the model, however, is that the asymptotic value of $ω$ at the walls is singular, necessitating the use of a contrived “sufficiently” large value for $ω$ as the boundary condition for its transport equation. Here, this invariably leads to the solution being sensitive to near wall grid spacing. While an acceptable solution for low order (finite volume) methods, the excessive near wall gradients lead to persistent numerical stability issues in high order codes. To alleviate the problem, specifically in the context of the high order spectral element code Nek5000, a regularized $k - ω$ approach was formulated in our prior work (Tomboulides et al., 2018). The formulation, however, relies on the use of wall distance and its gradients for modeling the closure terms and can pose problems for simulations in complex geometries. This work presents a novel implementation of the $k - τ$ RANS model in Nek5000, where $τ = 1/ω$, eliminating the need for regularization, owing to the asymptotically bounded behavior of the source terms in the $τ$ transport equation, and also eliminating dependence on wall distance. Robustness and stability of the $k - τ$ model is ensured through implicit treatment of the source terms and their careful numerical implementation and demonstrated through several cases aimed at verification and validation. Studies include both canonical and engineering relevant problems, viz., turbulent channel flow, pipe flow, backward facing step, flow over NACA 0012 airfoil and flow in a T-junction. Results from the $k - τ$ model are shown to be consistent with regularized $k - ω$ model and also with the $k - ω$ SST model in OpenFOAM (for select studies). Comparison with experimental data is also shown, where available, to bolster validation efforts for the $k - τ$ model implementation through prediction of key turbulent quantities of interest.

Nek5000↗

Data-Driven RANS Turbulence Closures for Forced Convection Flow in Reactor Downcomer Geometry

Recent progress in data-driven turbulence modeling has shown its potential to enhance or replace traditional equation-based Reynolds-averaged Navier-Stokes (RANS) turbulence models. Here, this work utilizes invariant neural network (NN) architectures to model Reynolds stresses and turbulent heat fluxes in forced convection flows (when the models can be decoupled). As the considered flow is statistically one dimensional, the invariant NN architecture for the Reynolds stress model reduces to the linear eddy viscosity model. To develop the data-driven models, direct numerical and RANS simulations in vertical planar channel geometry mimicking a part of the reactor downcomer are performed. Different conditions and fluids relevant to advanced reactors (sodium, lead, unitary-Prandtl-number fluid, and molten salt) constitute the training database. The models enabled accurate predictions of velocity and temperature, and compared to the baseline k–τ turbulence model with the simple gradient diffusion hypothesis, do not require tuning of the turbulent Prandtl number. The data-driven framework is implemented in the open-source graphics processing unit–accelerated spectral element solver nekRS and has shown the potential for future developments and consideration of more complex mixed convection flows.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Toward Verification of RANS Simulations of the T-Tube Modular Divertor Using Large Eddy Simulations of Impinging Turbulent Plane Jets

Turbulent impinging jets have been proposed to cool high heat flux plasma-facing components such as the solid tungsten target plates of the divertor in long-pulse magnetic fusion energy reactors. In particular, the T-tube modular divertor, originally developed by the ARIES Team, consists of two concentric cylindrical tubes where helium flows through a slot in the inner tube, forming an approximately planar jet that impinges upon and cools the inner surface of the pressure boundary (namely, the outer tube) and the ~15-cm 2 plasma-facing W target. The objective of this work is to demonstrate that large eddy simulations (LESs) accurately simulate the thermal transport in canonical flows that comprise the cooling flow in the T-tube, as well as validate temperatures from LES with experimental measurements in a simplified T-tube geometry. Wall‑resolved LESs, validated by experimental data and verified by direct numerical simulations (DNSs), provide benchmark data for two canonical flows in the T‑tube, namely, planar impinging and wall jets, for Reynolds numbers Re B = 4 × 10 3 to 2 × 10 4 . Our LES results are within 4% to 12% root-mean-square error (RMSE) of surface Nusselt number distributions (Nu) from experiments and DNSs. The validated LES results are then used as the ground truth to evaluate four Reynolds‑averaged Navier-Stokes (RANS) turbulence closures, namely, the k‑ω SST, realizable k‑ε, GEKO, and γ‑SST models. The k‑ω SST model has the best overall performance in terms of heat transfer, giving surface Nu within 12% RMSE of the LES results for high‑ReB impinging jets and reduced overprediction in the wall‑jet region. The GEKO model with default constants has the next best performance, providing slightly better Nu predictions for low ReB impinging jets (versus k-ω SST) but worse overall performance over the full range of ReB studied here. The realizable k‑ε turbulence model significantly overestimates turbulence near the stagnation point, while the γ‑SST model suppresses near‑wall production, biasing the simulations toward simulating laminar surface heat transfer. Simulations of the simplified T‑tube show that LES and RANS simulations with the k‑ω SST model give nearly identical average heat transfer coefficients (HTCs) over the impingement surface. The realizable k‑ε model predicts significantly lower wall temperatures due to overestimation of HTC in the outlet flow.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Eucalyptus grandis MYB‐Like and RAN‐Like Zinc Finger Proteins Display Dual Roles in Regulating Plant Immunity and Symbiosis Pathways

Plant roots live in constant contact with diverse microbes in the soil. Plant fitness, therefore, relies on signaling pathways that mount an effective immune response against pathogens while fostering mutualistic symbioses. Plant pathways, and specifically immune genes that may act as "switches," discriminating between pathogenic or mutualistic fungi, remain largely unknown. Using Eucalyptus grandis as a model system, we investigate alterations to the root transcriptomic landscape during pre-symbiosis with either the pathogen Armillaria luteobubalina or the mutualistic fungus Pisolithus microcarpus. Comparative analyses identified three strongly counter-regulated genes that may act as immune switches to accommodate or to repress fungal colonization. We characterized two of these, a MYB-like and RAN-like zinc finger protein, using a transgenic approach and demonstrated that they have bifunctional roles in the regulation of cell death and a hypersensitive-like response, depending on the lifestyle of the associated fungus. Using co-expression network analysis, we identified hypothetical pathways correlated to these genes. We functionally validated these predictions using plants with transgenic roots with increased or decreased transcription of these genes, thereby showing the power of co-expression networks as an a priori approach to identify key immune response pathways in plants. Overall, our results demonstrate that prior to physical contact with microbes, MYB-like and RAN-like zinc finger proteins are key regulators of plant immune signaling that respond to fungal signals and enable or repress symbiotic establishment.

mycorrhizal fungi↗

Applying and validating the RANS-3D flow-solver for evaluating a subsonic serpentine diffuser geometry

Subsonic inlet ducts for advanced, high-performance aircraft are evolving towards complex three-dimensional shapes for reasons of overall integration and weight. These factors lead to diffuser geometries that may sacrifice inlet performance, unless careful attention to design details and boundary layer management techniques are employed. The ability of viscous computational fluid dynamic (CFD) analysis of such geometries to aid the aircraft configurator in this complex design problem is herein examined. The RANS-3D Reynolds-Averaged Navier-Stokes solver is applied to model the complex flowfield occurring in a representative diffuser geometry and the solutions are compared to experimental results from a static test of the inlet duct. The computational results are shown to compare very favorably with experimental results over a range of mass flow rates, including those involving large amounts of separation in the diffuser. In addition, a novel grid topology is presented, and two turbulence models are evaluated in this study as part of the RANS-3D code.

Fletcher, Michael J.↗

Integrated RANS-LES Computations of Turbomachinery Components: Generic Compressor/Diffuser

The goal of the current study is to apply the coupled RANS-LES approach to a compressor-diffuser geometry of a gas turbine. This flow configuration is important, since the outflow of the compressor alters the flow field in the subsequent diffuser. A detailed knowledge of the flow field in this section allows to optimize the difuser design in order to to achieve a decrease of pressure loss. Here, the NASA stage 35 compressor is coupled with a generic diffuser in order to assess the integrated RANS-LES approach.

Schlueter, J. U.↗

RANS and DES Computations for a Wing with Ice Accretion

A computational investigation was performed to assess the effectiveness of Detached Eddy Simulation (DES) as a tool for predicting icing effects. The AVUS code, a public domain flow solver, was employed to compute solutions for an iced wing configuration using DES and steady Reynolds Averaged Navier-Stokes (RANS) equation methodologies. The wing section considered here was a business jet airfoil (GLC305) with a 22.5-minute glaze ice accretion (944-ice shape). The section was extruded to form a rectangular planform. The model was mounted between two walls so no tip effects were considered. The numerical results were validated by comparison with experimental data for the same configuration. The time-averaged DES computations showed some improvement in lift and drag results near stall when compared to steady RANS results. However, comparisons of the flow field details did not show the level of agreement suggested by the integrated quantities. More validation is needed to determine what role DES can play as part of an overall icing effects prediction strategy.

Mogili, P.↗

RANS Analyses of Turbofan Nozzles with Wedge Deflectors for Noise Reduction

Computational fluid dynamics (CFD) was used to evaluate a promising concept for reducing the noise at take-off of dual-stream, turbofan nozzles. The concept, offset stream technology, reduces the jet noise observed on the ground by diverting (offsetting) the majority of the fan flow below the core flow, thickening this layer between the high velocity core flow and the ground observers. In this study a wedge placed in the internal fan stream is used as the diverter. Wind, a Reynolds Averaged Navier-Stokes (RANS) code, was used to analyze the flowfield of the exhaust plume and to calculate nozzle performance. Results showed that the wedge effectively diverts the fan flow and the turbulent kinetic energy on the observer side of the nozzle is reduced. The reduction in turbulent kinetic energy should correspond to a reduction in noise. The blockage due to the wedge reduces the fan massflow proportional to its blockage and the overall thrust is consequently reduced. The CFD predictions are in very good agreement with experimental data. This noise reduction concept shows promise for reduced jet noise at a small reduction in thrust. It has been demonstrated that RANS CFD can be used to optimize this concept.

DeBonis, James R.↗

Validation of Heat Transfer and Film Cooling Capabilities of the 3-D RANS Code TURBO

The capabilities of the 3-D unsteady RANS code TURBO have been extended to include heat transfer and film cooling applications. The results of simulations performed with the modified code are compared to experiment and to theory, where applicable. Wilcox s k-turbulence model has been implemented to close the RANS equations. Two simulations are conducted: (1) flow over a flat plate and (2) flow over an adiabatic flat plate cooled by one hole inclined at 35 to the free stream. For (1) agreement with theory is found to be excellent for heat transfer, represented by local Nusselt number, and quite good for momentum, as represented by the local skin friction coefficient. This report compares the local skin friction coefficients and Nusselt numbers on a flat plate obtained using Wilcox's k-model with the theory of Blasius. The study looks at laminar and turbulent flows over an adiabatic flat plate and over an isothermal flat plate for two different wall temperatures. It is shown that TURBO is able to accurately predict heat transfer on a flat plate. For (2) TURBO shows good qualitative agreement with film cooling experiments performed on a flat plate with one cooling hole. Quantitatively, film effectiveness is under predicted downstream of the hole.

Shyam, Vikram↗

Coupling Turbulence in Hybrid LES-RANS Techniques

A formulation is proposed for hybrid LES-RANS computations that permits accurate computations during resolution changes, so that resolution may be changed at will in order to employ only as much resolution in each subdomain as is required by the physics. The two components of this formulation, establishing the accuracy of a hybrid model at constant resolutions throughout the RANS-to-LES range and maintaining that accuracy when resolution is varied, are demonstrated for decaying, homogeneous, isotropic turbulence.

Woodruff, Stephen L.↗

Model-Invariant Hybrid LES-RANS Computation of Separated Flow Past Periodic Hills

The requirement that physical quantities not vary with a hybrid LESRANS model's blending parameter imposes conditions on the computation that lead to better results across LES-RANS transitions. This promises to allow placement of those transitions so that LES is performed only where required by the physics, improving computational efficiency. The approach is applied to separated flow past periodic hills, where good predictions of separation-bubble size are seen due to the gradual, controlled, LES-RANS transition and the resulting enhanced near-wall eddy viscosity.

Woodruff, Stephen↗

Predictions of Slat Noise from the 30P30N at High Angles of Attack Using Zonal Hybrid RANS-LES

Aeroacoustic predictions of slat noise from the 30P30N three-element high-lift system at high angles of attack are presented using a zonal hybrid RANS-LES method. The simulations are part of the 5th AIAA Benchmark problems for Airframe Noise Computations (BANC-V) Workshop. An economical approach utilizing structured overset grids with spatially varying span-wise grid resolution and a high-order accurate finite difference method is described. The method is utilized for near-field predictions at three angles of attack: α = 5.5, 9.5, and 14.0 degrees. Far-field noise is obtained by propagating the near-field solution using a permeable surface Ffowcs Williams-Hawkings (FWH) method. Good agreement is obtained with both near-field and far-field Power Spectral Density (PSD) data from an experimental study of the 30P30N in the 2m x 2m Kevlar-wall wind tunnel at the Japan Aerospace Exploration Agency (JAXA). Specifically, the reduction in narrow band peaks and overall broadband noise levels with increasing angle of attack is captured well using the zonal hybrid RANS-LES method.

Housman, Jeffrey A.↗

On Boundary-Value Problems for RANS Equations and Two-Equation Turbulence Models

Currently, in engineering computations for high Reynolds number turbulent flows, turbulence modeling continues to be the most frequently used approach to represent the effects of turbulence. Such models generally rely on solving either one or two transport equations along with the Reynolds-Averaged Navier–Stokes (RANS) equations. The solution of the boundary-value problem of any system of partial differential equations requires the complete delineation of the equations and the boundary conditions, including any special restrictions and conditions. In the literature, such a description is often incomplete, neglecting important details related to the boundary conditions and possible restrictive conditions, such as how to ensure satisfying prescribed values of the dependent variables of the transport equations in the far field of a finite domain. In this article, we discuss the possible influence of boundary values, as well as near-field and far-field behavior, on the solution of the RANS equations coupled with transport equations for turbulence modeling. In so doing, we defne the concept of a welldefined boundary-value problem. Additionally, a three-dimensional, rather than a simpler one-dimensional analysis is performed to analyze the near-wall and far-field behavior of the turbulence model variables. This allows an assessment of the decay rate of these variables required to realize the boundary conditions in the far field. This paper also addresses the impact of various transformations of two-equation models (e.g., the model of Wilcox) to remove the singular behavior of the dissipation rate (ω) at the surface boundary. Finally, the issue of well-posedness regarding the governing equations is considered. A compelling argument (although not a proof) for ill-posedness is made for both direct and inverse problems.

Navier–Stokes equations↗

Demonstration of RANS models with wall functions in the spectral element code Nek5000

The spectral element based computational fluid dynamics (CFD) code Nek5000 has been traditionally used for high-fidelity applications, such as direct numerical simulation (DNS) and large eddy simulation (LES). These techniques require very fine numerical resolution to accurately capture turbulent fluctuations which can be prohibitively expensive for users without access to leadership class computing facilities. For broader application and adoption, significant effort has been invested to develop Reynolds-averaged Navier–Stokes (RANS) capabilities in Nek5000. Here, this work presents details of the implementation and demonstration of the standard wall functions for the κ–τ model in Nek5000. Results using the wall-modeled approach are compared to a wall-resolved approach for cases with negligible pressure gradient, viz., channel flow, pipe flow and flow in a reactor subchannel. Results show reasonably good agreement between the two approaches for friction factor and Nusselt number. Some expected differences are identified near the wall. These cases demonstrate the potential for significant computational savings by using much coarser meshes for the wall-modeled approach, with only minor differences between the predicted result. Additionally, several Reynolds numbers up to 1,000,000 are demonstrated for pipe flow and predicted friction factors and Nusselt numbers compared well to available correlations, with the worst below 10%. As the Reynolds number is increased, better agreement is observed between the correlations and the wall-modeled approach. In addition, flow in a molten salt fast reactor (MSFR) core is considered which features an adverse pressure gradient and flow separation. It showcases the inability of standard wall functions to accurately predict flows with adverse pressure gradients. The results, however, match reasonably well in trend in regions of the flow where the boundary layer is attached. Ongoing research is dedicated to include a pressure gradient correction to wall functions to improve the accuracy of flows with separation or reattachment and adverse or favorable pressure gradients.

42 ENGINEERING↗

Machine Learning–Guided Boolean Matrix Inference for Real-Time O-RAN Conflict Detection

Open Radio Access Networks (O-RAN) are emerging, software-driven cellular architectures that promote flexibility by enabling components from different vendors to interoperate. Multiple control applications called xApps can independently adjust network parameters in near real time, often without awareness of each other's actions. This creates a system highly prone to unintended conflicts and performance degradation due to the inherent complexity of such openness. To model such systems and ultimately prevent or mitigate xApp conflicts, it is essential to understand the dynamic relationships between xApps (A), the control parameters they adjust (P), and the resulting KPI responses (K). While the mappings from A to P and from K to A can often be derived from xApp specifications, the relationship from P to K is typically hidden within the system’s dynamics and must be inferred from observed data. We propose a novel data-driven Boolean inference framework that uncovers the hidden P?K dependencies using machine learning and interpretable rule induction. Continuous parameters and KPIs are first binarized using decision tree classifiers, and a binary influence matrix L is then inferred by solving Boolean matrix equations over time. This compact representation improves interpretability and enables real-time tracking of dynamically evolving parameter-KPI dependencies. We demonstrate the effectiveness of our method in a realistic mobile handover scenario, where it accurately recovers the underlying logic and enables proactive conflict detection.

42 - ENGINEERING↗

Generating a Grid for Unstructured RANS Simulations of Jet Flows

A study has been performed to determine best practices for generating unstructured grids for Reynolds-Averaged Navier-Stokes (RANS) simulations of jet flows. The Axisymmetric Near-Sonic Jet Case from the Turbulence Modeling Resource was used for this study: a Mach 0.985 flow through the 2-in. diameter Acoustic Reference Nozzle (ARN2). Simulations were run with FUN3D and used the Menter Shear Stress Transport (SST-V), Spalart-Allmaras (S-A), and k-kL turbulence models. The axial velocity and turbulent kinetic energy fields in the jet plume of the unstructured grid solutions were compared to those of the baseline structured grid solution provided by the Turbulence Modeling Resource. Only solutions using grids with structured-like elements in the jet plume showed good agreement with the baseline structured grid solution. Using the SST-V turbulence model, the fully unstructured grid solutions predicted the jet potential core to decay upstream of the baseline solution. With the S-A turbulence model, the unstructured grid solutions predicted the jet potential core to decay upstream of the baseline solution. The solutions using the k-kL turbulence model seemed less sensitive to grid topology. Nozzle massflow and thrust performance were also compared for all simulations. Based on the results of this study, it is currently recommended that structured-like grid elements are used in the plumes of jet flows; unstructured grid elements can be used elsewhere.

RANS Simulations; Unstructured Grids; Jet Flows↗