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Multiple Epstein-Barr virus infections in healthy individuals

We employed a newly developed genotyping technique with direct representational detection of LMP-1 gene sequences to study the molecular epidemiology of Epstein-Barr virus (EBV) infection in healthy individuals. Infections with up to five different EBV genotypes were found in two of nine individuals studied. These results support the hypothesis that multiple EBV infections of healthy individuals are common. The implications for the development of an EBV vaccine are discussed.

Evaluation Studies

The American mobile satellite system

During 1989, the American Mobile Satellite Corporation (AMSC) was authorized to construct, launch, and operate satellites to provide mobile satellite services (MSS) to the U.S. and Puerto Rico. The AMSC has undertaken three major development programs to bring a full range of MSS services to the U.S. The first program is the space segment program that will result in the construction and launch of the satellites as well as the construction and installation of the supporting ground telemetry and command system. The second segment will result in the specification, design, development, construction, and installation of the Network Control System necessary for managing communications access to the satellites, and the specification and development of ground equipment for standard circuit switched and packet switched communications services. The third program is the Phase 1 program to provide low speed data services within the U.S. prior to availability of the AMSC satellites and ground segment. Described here are the present status and plans for these three programs as well as an update on related business arrangements and regulatory matters.

Garner, William B.

Wake Vortex Wingtip-Turbine Powered Circulation Control High-Lift System

NASA s Vehicle Systems Program is investing in aeronautics technology development across six vehicle sectors, in order to improve future air travel. These vehicle sectors include subsonic commercial transports, supersonic vehicles, Uninhabited Aerial Vehicles (UAVs), Extreme Short Takeoff and Landing (ESTOL) vehicles, Rotorcraft, and Personal Air Vehicles (PAVs). While the subsonic transport is firmly established in U.S. markets, the other vehicle sectors have not developed a sufficient technology or regulatory state to permit widespread, practical use. The PAV sector has legacy products in the General Aviation (GA) market, but currently only accounts for negligible revenue miles, sales, or market share of personal travel. In order for PAV s to ever capture a significant market, these small aircraft require technologies that permit them to be less costly, environmentally acceptable, safer, easier to operate, more efficient, and less dependent on large support infrastructures.

Moore, Mark D.

NASA Kennedy Space Center: Contributions to Sea Turtle Science and Conservation

The National Aeronautics and Space Administration (NASA) is a United States (US) federal agency that oversees US space exploration and aeronautical research. NASA's primary launch site, Kennedy Space Center (KSC) is located along the east coast of Florida, on Cape Canaveral and the western Atlantic Ocean. The natural environment within KSC's large land boundaries, not only functions as an extensive safety buffer-area, it performs simultaneously as a wildlife refuge and a national seashore. In the early 1960s, NASA was developing KSC for rocket launches and the US was establishing an awareness of, and commitment to protecting the environment. The US began creating regulations that required the consideration of the environment when taking action on federal land or with federal funds. The timing of the US Endangered Species Act (1973), the US National Environmental Policy Act (1972), coincided with the planning and implementation of the US Space Shuttle Program. This resulted in the first efforts to evaluate the impacts of space launch operation operations on waterways, air quality, habitats, and wildlife. The first KSC fauna and flora baseline studies were predominantly performed by University of Central Florida (then Florida Technological University). Numerous species of relative importance were observed and sea turtles were receiving regulatory review and protection as surveys by Dr. L Ehrhart (UCF) from 1973-1978 described turtles nesting along the KSC beaches and foraging in the KSC lagoon systems. These data were used in the first NASA Environmental Impact Statement for the Space Transportation System (shuttle program) in 1980. In 1982, NASA began a long term ecological monitoring program with contracted scientists on site. This included efforts to track sea turtle status and trends at KSC and maintain protective measures for these species. Many studies and collaborations have occurred on KSC over these last 45 years with agencies (USFWS, NOAA, NAVY), students, and universities (UCF, University of Toronto, Texas A&M, UF). This presentation will review the various studies and collaborations on sea turtles at KSC that include: nest distributions and success, stranding network development, aerial survey testing for nest counts, predator control assessments, the earliest baseline blood chemistry health determinations on nesting females, stress hormones in nesting females, multi-year study of hatchling sex ratios, genetics, species composition, abundance and distribution of in-water juveniles, turtle cold stun response, exterior lighting impacts and control, and satellite tag tracking of post-nesting turtles in the vicinity of near shore shoals and sand mining sites. Through these studies, monitoring, and recommendations, KSC has provided excellent stewardship and protection of the local environment. While conducting its space program mission, KSC has also made significant contributions of information for agencies charged with the conservation and management of these species

Provancha, Jane A.

Research papers and publications (1981-1987): Workload research program

An annotated bibliography of the research reports written by participants in NASA's Workload Research Program since 1981 is presented, representing the results of theoretical and applied research conducted at Ames Research Center and at universities and industrial laboratories funded by the program. The major program elements included: 1) developing an understanding of the workload concept; 2) providing valid, reliable, and practical measures of workload; and 3) creating a computer model to predict workload. The goal is to provide workload-related design principles, measures, guidelines, and computational models. The research results are transferred to user groups by establishing close ties with manufacturers, civil and military operators of aerospace systems, and regulatory agencies; publishing scientific articles; participating in and sponsoring workshops and symposia; providing information, guidelines, and computer models; and contributing to the formulation of standards. In addition, the methods and theories developed have been applied to specific operational and design problems at the request of a number of industry and government agencies.

Hart, Sandra G.

Molecular Mechanisms Regulating Muscle Fiber Composition Under Microgravity

The overall goal of this project is to reveal the molecular mechanisms underlying the selective and debilitating atrophy of specific skeletal muscle fiber types that accompanies sustained conditions of microgravity. Since little is currently known about the regulation of fiber-specific gene expression programs in mammalian muscle, elucidation of the basic mechanisms of fiber diversification is a necessary prerequisite to the generation of therapeutic strategies for attenuation of muscle atrophy on earth or in space. Vertebrate skeletal muscle development involves the fusion of undifferentiated mononucleated myoblasts to form multinucleated myofibers, with a concomitant activation of muscle-specific genes encoding proteins that form the force-generating contractile apparatus. The regulatory circuitry controlling skeletal muscle gene expression has been well studied in a number of vertebrate animal systems. The goal of this project has been to achieve a similar level of understanding of the mechanisms underlying the further specification of muscles into different fiber types, and the role played by innervation and physical activity in the maintenance and adaptation of different fiber phenotypes into adulthood. Our recent research on the genetic basis of fiber specificity has focused on the emergence of mature fiber types and have implicated a group of transcriptional regulatory proteins, known as E proteins, in the control of fiber specificity. The restriction of E proteins to selected muscle fiber types is an attractive hypothetical mechanism for the generation of muscle fiber-specific patterns of gene expression. To date our results support a model wherein different E proteins are selectively expressed in muscle cells to determine fiber-restricted gene expression. These studies are a first step to define the molecular mechanisms responsible for the shifts in fiber type under conditions of microgravity, and to determine the potential importance of E proteins as upstream targets for the effects of weightlessness. In the past year we have determined that the expression of E Proteins is restricted to specific fiber types by post-transcriptional mechanisms. By far, the most prevalent mechanism of cellular control for achieving post-transcriptional regulation of gene expression is selective proteolysis -through the ubiquitin -proteasome pathway. Steady-state levels of HEB message are similar in all fast and slow skeletal muscle fiber types, yet the protein is restricted to Type IIX fibers. HEB appears to be a nodal point for regulating fiber-specific transcription, as expression of the transcription factor is regulated at the post-transcriptional level. It is not clear at present whether the regulation is at the level of protein synthesis or degradation. We are now poised to evaluate the biological role of ubiquitination in fiber specific-gene expression by controlling the post-transcriptional expression of E Proteins. The use of metabolic labelling and pharmacological inhibitors of the ubiquitin pathway will be used to identify the mode of regulation of the Type IIX expression pattern. The potential role of specific kinases in effecting the restriction of HEB expression will be examined by using both inhibitors and activators. The results of these studies will provide the necessary information to evaluate the biological role of E proteins in controlling fiber type transitions, and in potentially attenuating the atrophic effects of microgravity conditions. We have also recently shown that ectopic expression of the HEB protein transactivates the Type IIX-specific skeletal a-actin reporter. The 218 bp skeletal a-actin promoter drives transgene expression solely in mature Type IIX fibers. A mouse also carrying the transgene MLCI/HEB (which ectopically expresses the E Protein HEB in Type IIB fibers) forces expression of the skeletal a-actin reporter gene in Type IIB fibers. We can now dissect the composition of this fiber-specific cis-element. The skeletal a-actin promoter is quite compact and has been extensively characterized in vitro for activity and binding factors. The single E box may act as a binding target of myogenic factor/HEB heterodimer to allow for IIX expression. The HEB transcription factor may recognize either the precise flanking sequences of the E Box, or perhaps interacting with other proteins bound nearby, and activating expression in Type IIX fibers. This E box will be both ablated, and alternatively, as ablation may well destroy any muscle-specific transcriptional activity, flanking sequences substituted with those surrounding the E box (El) of the myogenin promoter. Modification of fiber-specific transgene expression will be tested in transgenic mice. The results of these studies will provide basic information on the regulatory circuitry underlying fiber specificity, and will form the basis for building appropriate transgenic regulatory cassettes to effect fiber transitions in subsequent experimental manipulations on unweighted muscles.

Rosenthal, Nadia A.

Remediation Technology Collaboration Development

This slide presentation reviews programs at NASA aimed at development at Remediation Technology development for removal of environmental pollutants from NASA sites. This is challenging because there are many sites with different environments, and various jurisdictions and regulations. There are also multiple contaminants. There must be different approaches based on location and type of contamination. There are other challenges: such as costs, increased need for resources and the amount of resources available, and a regulatory environment that is increasing.

Mahoney, John

The Civil Aviation Sector in Lebanon: Institutional Reforms - Part 1

Civil aviation is one of the key contributors to a successful economic system. This has been recognized within Lebanon, which is undertaking developing a new civil aviation strategy encompassing a program of organizational reform, coordinated internationally, to meet the challenges of the new century. Such strategy is vital, as it will provide a coherent vision for the sector, compliment the extensive investments deployed by Lebanon in its aviation infrastructure, and guide future planning and investments. The proposed Civil Aviation Strategy for Lebanon has two major components: (1) institutional reform aiming at creating effective overall legal and regulatory frameworks in-line with current international best practice; and (2) implementation of liberalization measures and open skies policy. This paper aims to: (1) present Lebanon's current institutional arrangements; (2) review the institutional arrangements in key select countries (in order to define current trends in best institutional practice); (3) discuss the proposed institutional reform (which are at the basis of Lebanon's Draft Civil Aviation Reform Law) while showing that they conform with the identified best institutional trends; and (4) outline an implementation plan. The Draft Law has been approved by the Council of Ministers and now awaits Parliamentary endorsement.

Baaj, M. Hadi

Environmentally regulated aerospace coatings

Aerospace coatings represent a complex technology which must meet stringent performance requirements in the protection of aerospace vehicles. Topcoats and primers are used, primarily, to protect the structural elements of the air vehicle from exposure to and subsequent degradation by environmental elements. There are also many coatings which perform special functions, i.e., chafing resistance, rain erosion resistance, radiation and electric effects, fuel tank coatings, maskants, wire and fastener coatings. The scheduled promulgation of federal environmental regulations for aerospace manufacture and rework materials and processes will regulate the emissions of photochemically reactive precursors to smog and air toxics. Aerospace organizations will be required to identify, qualify and implement less polluting materials. The elimination of ozone depleting chemicals (ODC's) and implementation of pollution prevention requirements are added constraints which must be addressed concurrently. The broad categories of operations affected are the manufacture, operation, maintenance, and repair of military, commercial, general aviation, and space vehicles. The federal aerospace regulations were developed around the precept that technology had to be available to support the reduction of organic and air toxic emissions, i.e., the regulations cannot be technology forcing. In many cases, the regulations which are currently in effect in the South Coast Air Quality Management District (SCAQMD), located in Southern California, were used as the baseline for the federal regulations. This paper addresses strategies used by Southern California aerospace organizations to cope with these regulatory impacts on aerospace productions programs. All of these regulatory changes are scheduled for implementation in 1993 and 1994, with varying compliance dates established.

Morris, Virginia L.

Cooperative research and development opportunities with the National Cancer Institute

The Office of Technology Development (OTD) of the National Cancer Institute (NCI) is responsible for negotiating Cooperative Research and Development Agreements (CRADAs), whereby the knowledge resulting from NCI investigators' government-sponsored research is developed in collaboration with universities and/or industry into new products of importance for the diagnosis and treatment of cancer and acquired immunodeficiency syndrome (AIDS). The NCI has recently executed a unique 'clinical trials' CRADA and is developing a model agreement based upon it for the development and commercialization of products for the diagnosis and treatment of cancer and AIDS. NCI drug screening, preclinical testing, clinical trials, and AIDS program capabilities form the basis for this new technology development/technology transfer vehicle. NCI's extensive drug screening program and 'designer foods' program serve as potential sources of investigational new drugs (INDs) and cancer preventatives. Collaborations between NCI and pharmaceutical companies having the facilities, experience, and expertise necessary to develop INDs into approved drugs available to the public are being encouraged where the companies have proprietary rights to INDs, or where NCI has proprietary rights to INDs and invites companies to respond to a collaborator announcement published in the Federal Register. The joint efforts of the NCI and the chosen collaborator are designed to generate the data necessary to obtain pharmaceutic regulatory approval from the Food and Drug Administration (FDA) to market the drugs developed, and thereby make them available to health care providers for the diagnosis and treatment of cancer and AIDS.

Sybert, Kathleen

Overview of spaceflight immunology studies

The effects of spaceflight and analogues of spaceflight are discussed here and in nine accompanying articles. In this summary we present spaceflight studies with human subjects, animal subjects, and cell cultures and we review ground-based systems used to model the observed effects of spaceflight on the immune system. Human paradigms include bed rest, academic or psychological stress, physical stress, hypobaric or high altitude stress, and confinement. Animal models include antiorthostatic and orthostatic suspension, hypobarism, and confinement. The ten manuscripts in this collection were selected to provide a summary that should give the reader an overview of the various activities of spaceflight immunology researchers throughout the history of space travel. This manuscript identifies the major contributors to the study of spaceflight immunology, explains what types of studies have been conducted, and how they have changed over the years. Also presented is a discussion of the unusual limitations associated with spaceflight research and the efforts to develop appropriate ground-based surrogate model systems. Specific details, data, and mechanistic speculations will be held to a minimum, because they will be discussed in depth in the other articles in the collection.

NASA Discipline Number 18-10

Technology benefits for very large subsonic transports

Results are presented for a study conducted at the NASA Langley Research Center which examined the effects of advanced technologies on the performance and size of very large, long-range subsonic transports. The study was performed using the Flight Optimization System (FLOPS). a multidisciplinary system of computer programs for conceptual and preliminary design and evaluation of advanced aircraft concepts. A four-engine, baseline configuration representative of existing transport technology was defined having a payload of 412 passengers plus baggage and a design range of 7300 nmi. New 600, 800 and 1000-passenger advanced transport concepts were then developed and compared to the baseline configuration. The technologies examined include 1995 entry-into-service (ELS) engines, high aspect ratio supercritical wings, composite materials for the wing, fuselage and empennage, and hybrid laminar flow control (HLFC). All operational and regulatory requirements and constraints, such as fuel reserves, balanced field length, and second segment climb gradient were satisfied during the design process. The effect of the advanced technologies on the size, weight and performance of the advanced transport concepts are presented. In addition, the sensitivity of the takeoff gross weight of the advanced transport concepts to increases in design range and payload, and designing for stretch capability are also discussed.

Arcara, Philip C., Jr.

A Systematic Approach to Developing Paths Towards Airborne Vehicle Autonomy

Advanced Air Mobility (AAM) demands greater levels of aircraft autonomy than are currently implemented today. To enable this requirement, novel aircraft functionalities and technologies as well as supporting airworthiness and operational regulations are required. A structured method to derive a comprehensive list of aircraft level decision-making functions is defined and applied. The resulting function set is programmed into an ontology, and enables autonomous decision-making through the application of a defined decision-making process. Paths to implementing the functions are generated by applying a structured four step method. By surveying current technologies, airspace, procedures and regulations, the paths generation method defines incremental paths to autonomy that the current regulatory environment can support. Opportunities to implement novel technologies and functions are identified, and regulatory mechanisms supporting their implementation are underscored. The analysis provides the tools to further define aircraft functions and paths to their implementation, while demonstrating that for particular use cases, aircraft autonomy is attainable in the medium-term.

Paul Vajda

A Systems Analysis Approach to Understanding the Physiological Adaptation to Spaceflight

This book is a summary of interdisciplinary research (physiology, space medicine, engineering, computer science, mathematics) that spans two decades (1972-1992). The research was an attempt to use systems analysis, mathematical modeling, computer simulations, and database systems to integrate the biomedical spaceflight data that was being collected during this period. The goal of the effort was to achieve a better understanding of the human physiological response to short-term and long-term space travel. The activity was primarily devoted to analyzing the biomedical results of Skylab (1973-74), a series of three space missions which is still ranked as the most comprehensive of all long-term biomedical space studies to date. This work was begun as a coordinated effort between the National Aeronautics and Space Administration (Johnson Space Center) and the General Electric Company’s Space Division (Houston, TX). It was the intent that this multidisciplined, integrative approach could reveal aspects of the then-new science of microgravity adaptation that were not obvious by adhering to the traditional methodology of examining each organ system in isolation. Some joint work with the Russians, including the Apollo-Soyez test project and a joint bedrest study was also supported during this period. In the 1980’s the systems analysis group’s effort was redirected to support the science management of human and animal experiments on the Space Shuttle. A few examples from this era are also included in the book. Parts of this work have been published elsewhere, presented at technical meetings, and documented in reports with limited distribution. These publications will be referenced throughout the text and the interested reader is advised to use these as resource material where additional details are desired. The intent of this book is not to reproduce these documents but rather to present a coherent view of the integrative analysis under one cover. This volume contains the first detailed publication (other than in internal reports) of an extensive metabolic balance analysis of Skylab data, the development and validation of the “Whole-Body Algorithm,” and simulation studies of diverse hypogravic environments. An analysis of cardiovascular deconditioning and a description of the calcium regulatory model are also new. A long period has passed between the completion of the main body of work represented in this book and its publication in this form. It was inevitable that new research efforts would lead to developments related to the spaceflight problems addressed and thereby make some of our biomedical conclusions obsolete. Although in some cases reference to more recent work have been included, for the most part this book should be considered an historical summary demonstrating the approach and utility of systems analysis and computer modeling in the NASA Life Sciences program at the time the studies were conducted.

Leonard, Joel I.

Industry Initiated Core Safety Attributes for Human Spaceflight for the 7th IAASS Conference

Now that the NASA Commercial Crew Program (CCP) is beginning its full certification contract for crew transportation to the International Space Station (ISS), is it time for industry to embrace a minimum set of core safety attributes? Those attributes can then be evolved into an industry-led set of basic safety standards and requirements. After 50 years of human space travel sponsored by governments, there are two basic conditions that now exist within the international space industry. The first, there is enough of a space-faring history to encourage the space industry to design, develop and operate human spaceflight systems without government contracts for anything other than services. Second, industry is capable of defining and enforcing a set of industry-based safety attributes and standards for human spaceflight to low-Earth orbit (LEO). This paper will explore both of these basic conditions with a focus on the safety attributes and standards. In the United States, the Federal Aviation Administration (FAA) is now starting to dialogue with industry about the basic safety principles and attributes needed for potential future regulatory oversight. This process is not yet formalized and will take a number of years once approval is given to move forward. Therefore, throughout the next few years, it is an excellent time and opportunity for industry to collaborate together and develop the core set of attributes and standards. As industry engages and embraces a common set of safety attributes, then government agencies, like the FAA and NASA can use that industry-based product to strengthen their efforts on a safe commercial spaceflight foundation for the future. As the commercial space industry takes the lead role in establishing core safety attributes, and then enforcing those attributes, the entire planet can move away from governmental control of design and development and let industry expand safe and successful space operations in LEO. At that point the governmental agencies can focus on oversight of the industries' defined standards and enforcement for common welfare of the space-faring populous and overall public safety.

crew transportation

Quiet Sonic Booms: A NASA and Industry Progress Report

The purpose of this Oral Presentation is to present a progress report on NASA and Industry efforts related to Quiet Sonic Boom Program activities. This presentation will review changes in aircraft shaping to produce quiet supersonic booms and associated supersonic flight test methods and results. In addition, new flight test profiles have been recently developed that have allowed for the generation of sonic booms of varying intensity. These new flight test profiles have allowed for ground testing of the response of various building structures to sonic booms and the associated public acceptability to various sonic boom intensities. The new flight test profiles and associated ground measurement test methods will be reviewed. Finally, this Oral Presentation will review the International Regulatory requirements that would be involved to change aviation regulation and allow for overland quiet supersonic flight.

Larson, David Nils

Flight Testing of the Gulfstream Quiet Spike(TradeMark) on a NASA F-15B

Gulfstream Aerospace has long been interested in the development of an economically viable supersonic business jet (SBJ). A design requirement for such an aircraft is the ability for unrestricted supersonic flight over land. Although independent studies continue to substantiate that a market for a SBJ exists, regulatory and public acceptance challenges still remain for supersonic operation over land. The largest technical barrier to achieving this goal is sonic boom attenuation. Gulfstream's attention has been focused on fundamental research into sonic boom suppression for several years. This research was conducted in partnership with the NASA Aeronautics Research Mission Directorate (ARMD) supersonic airframe cruise efficiency technical challenge. The Quiet Spike, a multi-stage telescopic nose boom and a Gulfstream-patented design (references 1 and 2), was developed to address the sonic boom attenuation challenge and validate the technical feasibility of a morphing fuselage. The Quiet Spike Flight Test Program represents a major step into supersonic technology development for sonic boom suppression. The Gulfstream Aerospace Quiet Spike was designed to reduce the sonic boom signature of the forward fuselage for an aircraft flying at supersonic speeds. In 2004, the Quiet Spike Flight Test Program was conceived by Gulfstream and NASA to demonstrate the feasibility of sonic boom mitigation and centered on the structural and mechanical viability of the translating test article design. Research testing of the Quiet Spike consisted of numerous ground and flight operations. Each step in the process had unique objectives, and involved numerous test team members from the NASA Dryden Flight Research Center (DFRC) and Gulfstream Aerospace. Flight testing of the Quiet Spike was conducted at the NASA Dryden Flight Research Center on an F-15B aircraft from August, 2006, to February, 2007. During this period, the Quiet Spike was flown at supersonic speeds up to Mach 1.8 at the maximum design dynamic pressure of 685 pounds per square foot. Extension and retraction tests were conducted at speeds up to Mach 1.4. The design of the Quiet Spike to shape the forward shock wave environment of the aircraft was confirmed during near-field shock wave probing at Mach 1.4. Thirty-two flights were performed without incident and all project objectives were achieved. The success of the Quiet Spike Flight Test Program represents an important step towards developing commercial aircraft capable of supersonic flight over land within the continental United States and in international airspace.

Smolka, James W.

Extracellular matrix and growth factors in branching morphogenesis

The unifying hypothesis of the NSCORT in gravitational biology postulates that the ECM and growth factors are key interrelated components of a macromolecular regulatory system. The ECM is known to be important in growth and branching morphogenesis of embryonic organs. Growth factors have been detected in the developing embryo, and often the pattern of localization is associated with areas undergoing epithelial-mesenchymal interactions. Causal relationships between these components may be of fundamental importance in control of branching morphogenesis.

Non-NASA Center