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At least 55 records · Page 3

Enhanced root production in Haplopappus gracilis grown under spaceflight conditions

The production and growth of roots in two aseptically maintained clonal populations of Haplopappus gracilis (family Compositae), each with a distinctive pattern of root production, were studied after they had been exposed to space for 5 days aboard a NASA Space Shuttle. Total root production of both populations was 67-95% greater when compared with their Earth-grown controls. Roots were generated: (1) laterally from pre-formed roots, the tips of which had been severed at the time of plantlet insertion into a "horticultural foam" substrate supplied with a nutrient solution; (2) adventitiously from the basal or cut-end portion of shoots; (3) de novo, i.e. from primordial which were non-existent at the outset of the experiment. Roots grew in all directions in space but were uniformly positively gravitropic in ground controls. In space and on Earth, both clonal populations maintained their clone-specific root formation and growth characteristics and produced an equivalent amount of tissue when compared to each other. As on Earth, and as expected, there were fewer and shorter roots on plantlets that formed floral buds. The significance of altered moisture distribution in the "horticultural foam" substrate in space for root growth and the significance of our findings for growing plants in altered gravity environments are discussed.

Flight Experiment↗

A computer package for the design and eigenproblem solution of damped linear multidegree of freedom systems

Systems described by the matrix differental equation are considered. An interactive design routine is presented for positive definite mass, damping, and stiffness matrices. Designing is accomplished by adjusting the mass, damping, and stiffness matrices to obtain a desired oscillation behavior. The algorithm also features interactively modifying the physical structure of the system, obtaining the matrix structure and a number of other system properties. In case of a general system, where the M, C, and K matrices lack any special properties, a routine for the eigenproblem solution of the system is developed. The latent roots are obtained by computing the characteristic polynomial of the system and solving for its roots. The above routines are prepared in FORTRAN IV and prove to be usable for the machines with low core memory.

Ahmadian, M.↗

A morphometric analysis of cellular differentiation in caps of primary and lateral roots of Helianthus annuus

In order to determine if patterns of cell differentiation are similar in primary and lateral roots, I performed a morphometric analysis of the ultrastructure of calyptrogen, columella, and peripheral cells in primary and lateral roots of Helianthus annuus. Each cell type is characterized by a unique ultrastructure, and the ultrastructural changes characteristic of cellular differentiation in root caps are organelle specific. No major structural differences exist in the structures of the composite cell types, or in patterns of cell differentiation in caps of primary vs. lateral roots.

Non-NASA Center↗

Three-Dimensional Analysis of Nuclear Size, Shape and Displacement in Clover Root Cap Statocytes from Space and a Clinostat

Under normal (l-g) conditions the statocytes of root caps have a characteristic polarity with the nucleus in tight association with the proximal cell wall; but, in altered gravity environments including microgravity (mu-g) and the clinostat (c-g) movement of the nucleus away from the proximal cell wall is not uncommon. To further understand the cause of gravity-dependent nuclear displacement in statocytes, three-dimensional cell reconstruction techniques were used to precisely measure the volumes, shapes, and positions of nuclei in white clover (Trifolium repens) flown in space and rotated on a clinostat. Seeds were germinated and grown for 72 hours aboard the Space Shuttle (STS-63) in the Fluid Processing Apparatus (BioServe Space Technologies, Univ. of Colorado, Boulder). Clinorotation experiments were performed on a two-axis clinostat (BioServe). Computer reconstruction of selected groups of statocytes were made from serial sections (0.5 microns thick) using the ROSS (Reconstruction Of Serial Sections) software package (Biocomputation Center, NASA Ames Research Center). Nuclei were significantly displaced from the tops of cells in mu-g (4.2 +/- 1.0 microns) and c-g (4.9 +/- 1.4 microns) when compared to l-g controls (3.4 +/- 0.8 gm); but, nuclear volume (113 +/- 36 cu microns, 127 +/- 32 cu microns and 125 +/- 28 cu microns for l-g, mu-g and c-g respectively) and the ratio of nuclear volume to cell volume (4.310.7%, 4.211.0% and 4.911.4% respectively) were not significantly dependent on gravity treatment (ANOVA; alpha = 0.05). Three-dimensional analysis of nuclear shape and proximity to the cell wall, however, showed that nuclei from l-g controls appeared ellipsoidal while those from space and the clinostat were more spherically shaped. This change in nuclear shape may be responsible for its displacement under altered gravity conditions. Since the cytoskeleton is known to affect nuclear polarity in root cap statocytes, those same cytoskeletal elements could also control nuclear shape. This alteration in nuclear shape and position in mu-g and c-g when compared to l-g may lead to functional differences in the gravity signaling systems of plants subjected to altered gravity environments.

Smith, J.D.↗

Multipath errors in range rate measurement by a TDRS/VHF - GRARR

Range rate errors due to multipath reflection are calculated for a tracking and data relay satellite system using the VHF Goddard range and range rate (GRARR) system. At VHF the reflection is primarily specular, and the strength of the multipath relative to the direct path can be modeled in terms of the geometry and the surface characteristics, specifically the root-mean-square (rms) ocean wave height. The uplink and downlink multipath introduces phase jitter on the GRARR carrier and subcarrier. The derivation of these effects is reviewed leading to an expression for the rms range rate error. The derivation assumed the worst-case orbital configurations in which there was very little relative specular Doppler. This means that the specular multipath interference was not attenuated by the carrier and subcarrier PLL transfer functions. Curves of range rate error are presented as a function of grazing angle with wave height 0.3 to 0.7 meters and spacecraft altitude 100 to 700 miles as parameters.

Sohn, S. J.↗

Dynamic analysis of a class of mixed lumped-distributed parameter systems via numerical techniques

The modeling via transfer matrices of mixed lumped-distributed parameter systems with feedback control is discussed and novel methods of obtaining information on the controlled system's dynamic response via numerical methods is presented. These methods utilize numerical searches in the complex plane for the roots of the transcendental characteristic equation to determine the closed loop system eigenvalues. The fast Fourier transform (FFT) algorithm is utilized but in the inverse fashion (from frequency domain to time domain). The information obtained is identical to that contained in a model in modal coordinates, and can be used to construct such a model. An interactive computer program to do the required calculation is described, as well as its application to a manipulator arm design problem.

Book, W. J.↗

Effects of root-zone acidity on utilization of nitrate and ammonium in tobacco plants

Tobacco (Nicotiana tabacum L., cv. 'Coker 319') plants were grown for 28 days in flowing nutrient culture containing either 1.0 mM NO3- or 1.0 mM NH4+ as the nitrogen source in a complete nutrient solution. Acidities of the solutions were controlled at pH 6.0 or 4.0 for each nitrogen source. Plants were sampled at intervals of 6 to 8 days for determination of dry matter and nitrogen accumulation. Specific rates of NO3- or NH4+ uptake (rate of uptake per unit root mass) were calculated from these data. Net photosynthetic rates per unit leaf area were measured on attached leaves by infrared gas analysis. When NO3- [correction of NO-] was the sole nitrogen source, root growth and nitrogen uptake rate were unaffected by pH of the solution, and photosynthetic activity of leaves and accumulation of dry matter and nitrogen in the whole plant were similar. When NH4+ was the nitrogen source, photosynthetic rate of leaves and accumulation of dry matter and nitrogen in the whole plant were not statistically different from NO3(-) -fed plants when acidity of the solution was controlled at pH 6.0. When acidity for NH4(+) -fed plants was increased to pH 4.0, however, specific rate of NH4+ uptake decreased by about 50% within the first 6 days of treatment. The effect of acidity on root function was associated with a decreased rate of accumulation of nitrogen in shoots that was accompanied by a rapid cessation of leaf development between days 6 and 13. The decline in leaf growth rate of NH4(+) -fed plants at pH 4.0 was followed by reductions in photosynthetic rate per unit leaf area. These responses of NH4(+) -fed plants to increased root-zone acidity are characteristic of the sequence of responses that occur during onset of nitrogen stress.

NASA Discipline Life Support Systems↗

Effects of nonplanar outboard wing forms on a wing

It is possible for a constant span to obtain better aerodynamic performance from a wing with a nonplanar outboard wing form than from a wing with a planar outboard form, despite the added drag from the increased wetted area. Furthermore, the semispan rolling-moment characteristics indicate the lower wing-root bending moment for some nonplanar configurations. These conclusions are based on an experimental and computational investigation of the aerodynamic characteristics of planar and nonplanar outboard wing forms. Seven different configurations - planar rectangular, nonplanar rising arc, nonplanar drooping arc, planar sheared, sheared with dihedral, sheared with anhedral, and planar elliptical - were investigated for two different spans. Flow-visualization photographs indicate that there are three vortex systems associated with the sheared forms. The lower induced drag coefficients of nonplanar wings are believed to accrue from the movement of vorticity away from the center-of-span line, resulting, in some instances, in induced efficiencies higher than that of a planar elliptical wing. Flow surveys indicate that the effective span, as determined by the location of the tip vortex, might not be a sufficient yardstick of the induced performance of a nonplanar wing.

Naik, D. A.↗

Computer based imaging and analysis of root gravitropism

Two key issues in studies of the nature of the gravitropic response in roots have been the determination of the precise pattern of differential elongation responsible for downward bending and the identification of the cells that show the initial motor response. The main approach for examining patterns of differential growth during root gravitropic curvature has been to apply markers to the root surface and photograph the root at regular intervals during gravitropic curvature. Although these studies have provided valuable information on the characteristics of the gravitropic motor response in roots, their labor intensive nature limits sample size and discourages both high frequency of sampling and depth of analysis of surface expansion data. In this brief review we describe the development of computer-based video analysis systems for automated measurement of root growth and shape change and discuss some key features of the root gravitropic response that have been revealed using this methodology. We summarize the capabilities of several new pieces of software designed to measure growth and shape changes in graviresponding roots and describe recent progress in developing analysis systems for studying the small, but experimentally popular, primary roots of Arabidopsis. A key finding revealed by such studies is that the initial gravitropic response of roots of maize and Arabidopsis occurs in the distal elongation zone (DEZ) near the root apical meristem, not in the main elongation zone. Another finding is that the initiation of rapid elongation in the DEZ following gravistimulation appears to be related to rapid membrane potential changes in this region of the root. These observations have provided the incentive for ongoing studies examining possible links between potential growth modifying factors (auxin, calcium, protons) and gravistimulated changes in membrane potential and growth patterns in the DEZ.

NASA Discipline Plant Biology↗

Radar scattering laws for the lunar surface

Bistatic radar observations of quasi-specular scatter from the lunar surface at 13 and 116 cm wavelengths have been expressed in terms of probability densities of the underlying surface slope distributions. These show best agreement with a Hagfors scattering function, although other forms having predominantly Gaussian or exponential characteristics also occur. Estimates of root mean square (rms) surface slope derived from these function range from 4 deg rms in maria to at least 8 deg rms in highlands. These are values appropriate to 25 m horizontal scales and represent averages over tens of square kilometers of surface area. The effective scattering roughness varies with wavelength, most strongly in maria, where its behavior is consistent with that expected from an immature, i.e., unsaturated cratered surface. There is very little variation in scattering roughness with wavelength in highlands area.

Simpson, R. A.↗

Pipelaying Barges

The vessel pictured is the Hugh Gordon, a pipelaying barge designed and built by Brown & Root, Inc., Houston, Texas. The largest engineering and construction firm in the United States, Brown & Root is engaged in such activities as construction of chemical and petroleum plants, pulp and paper plants, highways and bridges, and offshore petroleum facilities. The computer program SHCP (Ship Hull Characteristics Program) is used by Brown & Root engineers as a tool in designing barges, such as the one shown, for offshore pipelaying projects. Made available to industry by NASA's Computer Software Management and Information Center (COSMIC), SHCP is a composite program designed to solve basic naval architecture problems and to assess the structural integrity and stability of a vessel design; it is used extensively by marine industry designers.

Source record↗

Infrared Images of Boundary Layer Transition on the D8 Transport Configuration in the LaRC 14- by 22-Foot Subsonic Tunnel

Grit, trip tape, or trip dots are routinely applied on the leading-edge regions of the fuselage, wings, tails or nacelles of wind tunnel models to trip the flow from laminar to turbulent. The thickness of the model's boundary layer is calculated for nominal conditions in the wind tunnel test to determine the effective size of the trip dots, but the flow over the model may not transition as intended for runs with different flow conditions. Temperature gradients measured with an infrared camera can be used to detect laminar to turbulent boundary layer transition on a wind tunnel model. This non-intrusive technique was used in the NASA Langley 14- by 22-Foot Subsonic Tunnel to visualize the behavior of the flow over a D8 transport configuration model. As the flow through the wind tunnel either increased to or decreased from the run conditions, a sufficient temperature difference existed between the air and the model to visualize the transition location (due to different heat transfer rates through the laminar and the turbulent boundary layers) for several runs in this test. Transition phenomena were visible without active temperature control in the atmospheric wind tunnel, whether the air was cooler than the model or vice-versa. However, when the temperature of the model relative to the air was purposely changed, the ability to detect transition in the infrared images was enhanced. Flow characteristics such as a wing root horseshoe vortex or the presence of fore-body vortical flows also were observed in the infrared images. The images of flow features obtained for this study demonstrate the usefulness of current infrared technology in subsonic wind tunnel tests.

Mason, Michelle L.↗

Experimental and Calculated Characteristics of Several NACA 44-series Wings with Aspect Ratios of 8, 10, and 12 and Taper Ratios of 2.5 and 3.5

The aerodynamic characteristics of seven unswept tapered wings were determined by calculation from two-dimensional data and by wind-tunnel tests in order to demonstrate the accuracy of the calculations and to show some of the effects of aspect ratio, taper ratio, and root thickness-chord ratio. The characteristics were calculated by the usual application of the lifting-line theory which assumes linear section lift curves and also by an application of the theory which allows the use of nonlinear lift curves. A correction to the lift for the effect of chord was made by using the Jones edge-velocity factor. The wings had aspect ratios of 8, 10, and 12, taper ratios of 2.5 and 3.5, and NACA 44-series airfoils.

WINGS, TAPERED - CHARACTERISTICS↗