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At least 55 records · Page 3

AmeriFlux US-VT2 Vermillion Tributary Paired Cropland – Site 2 (Corn/Soy; Cover Crops)

This is the AmeriFlux version of the carbon flux data for the site US-VT2 Vermillion Tributary Paired Cropland – Site 2 (Corn/Soy; Cover Crops). Site Description - US-VT2 is located on flat, actively managed farmland operated by working farmers, following a conventional no-till corn–soybean rotation in the U.S. Midwest that uses cover crops. US-VT2 is one of two paired working farm sites on the same property; both are managed using similar conventional practices, with the key difference being that the paired site (US-VT1) does not incorporate cover crops into its rotation. This paired design enables direct site-to-site comparisons to assess the impacts of cover cropping on carbon, water, and energy fluxes.

Key, Kesondra [Indiana University - Bloomington]↗

AmeriFlux US-SD1 Shatto Ditch Paired Cropland - Site 1 (Corn/Soy; No Cover Crops)

This is the AmeriFlux version of the carbon flux data for the site US-SD1 Shatto Ditch Paired Cropland - Site 1 (Corn/Soy; No Cover Crops). Site Description - US-SD1, located on mostly flat terrain with some gentle hills, is an actively managed farmland operated by working farmers following a conventional no-till corn–soybean rotation in the U.S. Midwest. US-SD1 is one of two paired working farm sites which are relatively close by; both are managed using similar conventional practices with the key difference being that the paired site (US-SD2) incorporates cover crops into its rotation, along with minor differentiating terrain and soil type, where US-SD2 is located on a gently sloped site with more sandy soils. This paired design enables direct site-to-site comparisons to assess the impacts of cover cropping on carbon, water, and energy fluxes.

Key, Kesondra [Indiana University - Bloomington]↗

AmeriFlux US-SD2 Shatto Ditch Paired Cropland - Site 2 (Corn/Soy; Cover Crops)

This is the AmeriFlux version of the carbon flux data for the site US-SD2 Shatto Ditch Paired Cropland - Site 2 (Corn/Soy; Cover Crops). Site Description - US-SD2, located on gently sloped terrain, is an actively managed farmland operated by working farmers following a conventional no-till corn–soybean rotation in the U.S. Midwest. US-SD2 is one of two paired working farm sites which are relatively close by; both are managed using similar conventional practices with the key difference being that the paired site (US-SD1) incorporates cover crops into its rotation, along with minor differentiating terrain and soil type, where US-SD1 is located on mostly flat terrain with some gentle hills. This paired design enables direct site-to-site comparisons to assess the impacts of cover cropping on carbon, water, and energy fluxes.

Key, Kesondra [Indiana University - Bloomington]↗

AmeriFlux FLUXNET-1F US-VT1 Vermillion Tributary Paired Cropland – Site 1 (Corn/Soy; No Cover Crops)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-VT1 Vermillion Tributary Paired Cropland – Site 1 (Corn/Soy; No Cover Crops). This is the FLUXNET version of the carbon flux data for the site US-VT1 Vermillion Tributary Paired Cropland – Site 1 (Corn/Soy; No Cover Crops) produced by applying the standard ONEFlux (1F) software. Site Description - US-VT1 is located on flat, actively managed farmland operated by working farmers, following a conventional no-till corn–soybean rotation in the U.S. Midwest. US-VT1 is one of two paired working farm sites on the same property; both are managed using similar conventional practices, with the key difference being that the paired site (US-VT2) incorporates cover crops into its rotation. This paired design enables direct site-to-site comparisons to assess the impacts of cover cropping on carbon, water, and energy fluxes.

Key, Kesondra [Indiana University - Bloomington]↗

AmeriFlux FLUXNET-1F US-VT2 Vermillion Tributary Paired Cropland – Site 2 (Corn/Soy; Cover Crops)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-VT2 Vermillion Tributary Paired Cropland – Site 2 (Corn/Soy; Cover Crops). This is the FLUXNET version of the carbon flux data for the site US-VT2 Vermillion Tributary Paired Cropland – Site 2 (Corn/Soy; Cover Crops) produced by applying the standard ONEFlux (1F) software. Site Description - US-VT2 is located on flat, actively managed farmland operated by working farmers, following a conventional no-till corn–soybean rotation in the U.S. Midwest that uses cover crops. US-VT2 is one of two paired working farm sites on the same property; both are managed using similar conventional practices, with the key difference being that the paired site (US-VT1) does not incorporate cover crops into its rotation. This paired design enables direct site-to-site comparisons to assess the impacts of cover cropping on carbon, water, and energy fluxes.

Key, Kesondra [Indiana University - Bloomington]↗

AmeriFlux FLUXNET-1F US-SD1 Shatto Ditch Paired Cropland - Site 1 (Corn/Soy; No Cover Crops)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-SD1 Shatto Ditch Paired Cropland - Site 1 (Corn/Soy; No Cover Crops). This is the FLUXNET version of the carbon flux data for the site US-SD1 Shatto Ditch Paired Cropland - Site 1 (Corn/Soy; No Cover Crops) produced by applying the standard ONEFlux (1F) software. Site Description - US-SD1, located on mostly flat terrain with some gentle hills, is an actively managed farmland operated by working farmers following a conventional no-till corn–soybean rotation in the U.S. Midwest. US-SD1 is one of two paired working farm sites which are relatively close by; both are managed using similar conventional practices with the key difference being that the paired site (US-SD2) incorporates cover crops into its rotation, along with minor differentiating terrain and soil type, where US-SD2 is located on a gently sloped site with more sandy soils. This paired design enables direct site-to-site comparisons to assess the impacts of cover cropping on carbon, water, and energy fluxes.

Key, Kesondra [Indiana University - Bloomington]↗

AmeriFlux FLUXNET-1F US-SD2 Shatto Ditch Paired Cropland - Site 2 (Corn/Soy; Cover Crops)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-SD2 Shatto Ditch Paired Cropland - Site 2 (Corn/Soy; Cover Crops). This is the FLUXNET version of the carbon flux data for the site US-SD2 Shatto Ditch Paired Cropland - Site 2 (Corn/Soy; Cover Crops) produced by applying the standard ONEFlux (1F) software. Site Description - US-SD2, located on gently sloped terrain, is an actively managed farmland operated by working farmers following a conventional no-till corn–soybean rotation in the U.S. Midwest. US-SD2 is one of two paired working farm sites which are relatively close by; both are managed using similar conventional practices with the key difference being that the paired site (US-SD1) incorporates cover crops into its rotation, along with minor differentiating terrain and soil type, where US-SD1 is located on mostly flat terrain with some gentle hills. This paired design enables direct site-to-site comparisons to assess the impacts of cover cropping on carbon, water, and energy fluxes.

Key, Kesondra [Indiana University - Bloomington]↗

Life Cycle Analysis of Enhanced Rock Weathering

This study aims to evaluate the life cycle impacts of weathering agricultural fields in the U.S. Midwest with igneous rock (dunite/olivine) and industrial waste minerals using literature and estimated data from a techno-economic analysis (TEA). The GWP impact with igneous rock and industrial waste materials ranges 34–263 and 23–180 kg CO2-eq/tonne CO2 sequestered, respectively, with comminution and transportation as the main environmental hotspots for igneous rock, and transportation for industrial waste.

Izar-Tenorio, Jorge↗

Niche Differentiation of Bacterial Versus Archaeal Soil Nitrifiers Induced by Ammonium Inhibition Along a Management Gradient

Soil nitrification, mediated mainly by ammonia oxidizing archaea (AOA) and bacteria (AOB), converts ammonium (NH 4 + ) to nitrite (NO 2 – ) and thence nitrate (NO 3 – ). To better understand ecological differences between AOA and AOB, we investigated the nitrification kinetics of AOA and AOB under eight replicated cropped and unmanaged ecosystems (including two fertilized natural systems) along a long-term management intensity gradient in the upper U.S. Midwest. For five of eight ecosystems, AOB but not AOA exhibited Haldane kinetics (inhibited by high NH 4 + additions), especially in perennial and successional systems. In contrast, AOA predominantly exhibited Michaelis-Menten kinetics, suggesting greater resistance to high nitrogen inputs than AOB. These responses suggest the potential for NH 4 + -induced niche differentiation between AOA and AOB. Additionally, long-term fertilization significantly enhanced maximum nitrification rates (V max ) in the early successional systems for both AOA and AOB, but not in the deciduous forest systems. This was likely due to pH suppression of nitrification in the acidic forest soils, corroborated by a positive correlation of V max with soil pH but not with amoA gene abundance. Results also demonstrated that soil nitrification potentials were relatively stable, as there were no seasonal differences. Overall, results suggest that (1) NH 4 + inhibition of AOB but not AOA could be another factor contributing to niche differentiation between AOA and AOB in soil, and (2) nitrification by both AOA and AOB can be significantly promoted by long-term nitrogen inputs.

59 BASIC BIOLOGICAL SCIENCES↗

Morpho-physiological and transcriptomic responses of field pennycress to waterlogging

Field pennycress (Thlaspi arvense) is a new biofuel winter annual crop with extreme cold hardiness and a short life cycle, enabling off-season integration into corn and soybean rotations across the U.S. Midwest. Pennycress fields are susceptible to winter snow melt and spring rainfall, leading to waterlogged soils. The objective of this research was to determine the extent to which waterlogging during the reproductive stage affected gene expression, morphology, physiology, recovery, and yield between two pennycress lines (SP32-10 and MN106). In a controlled environment, total pod number, shoot/root dry weight, and total seed count/weight were significantly reduced in SP32-10 in response to waterlogging, whereas primary branch number, shoot dry weight, and single seed weight were significantly reduced in MN106. This indicated waterlogging had a greater negative impact on seed yield in SP32-10 than MN106. We compared the transcriptomic response of SP32-10 and MN106 to determine the gene expression patterns underlying these different responses to seven days of waterlogging. The number of differentially expressed genes (DEGs) between waterlogged and control roots were doubled in MN106 (3,424) compared to SP32-10 (1,767). Functional enrichment analysis of upregulated DEGs revealed Gene Ontology (GO) terms associated with hypoxia and decreased oxygen, with genes in these categories encoding proteins involved in alcoholic fermentation and glycolysis. Additionally, downregulated DEGs revealed GO terms associated with cell wall biogenesis and suberin biosynthesis, indicating suppressed growth and energy conservation. Interestingly, MN106 waterlogged roots exhibited significant stronger regulation of these genes than SP32-10, displaying a more robust transcriptomic response overall. Together, these results reveal the reconfiguration of cellular and metabolic processes in response to the severe energy crisis invoked by waterlogging in pennycress.

ERF-VII↗

Predicting Biomass Yields of Advanced Switchgrass Cultivars for Bioenergy and Ecosystem Services Using Machine Learning

The production of advanced perennial bioenergy crops within marginal areas of the agricultural landscape is gaining interest due to its potential to sustainably produce feedstocks for biofuels and bioproducts while also improving the sustainability and resilience of commodity crop production. However, predicting the biomass yields of this production system is challenging because marginal areas are often relatively small and spread around agricultural fields and are typically associated with various abiotic conditions that limit crop production. Machine learning (ML) offers a viable solution as a biomass yield prediction tool because it is suited to predicting relationships with complex functional associations. The objectives of this study were to (1) evaluate the accuracy of commonly applied ML algorithms in agricultural applications for predicting the biomass yields of advanced switchgrass cultivars for bioenergy and ecosystem services and (2) determine the most important biomass yield predictors. Datasets on biomass yield, weather, land marginality, soil properties, and agronomic management were generated from three field study sites in two U.S. Midwest states (Illinois and Iowa) over three growing seasons. The ML algorithms evaluated in the study included random forests (RFs), gradient boosting machines (GBMs), artificial neural networks (ANNs), K-neighbors regressor (KNR), AdaBoost regressor (ABR), and partial least squares regression (PLSR). Coefficient of determination (R 2 ) and mean absolute error (MAE) were used to evaluate the predictive accuracy of the tested algorithms. Results showed that the ensemble methods, RF (R 2 = 0.86, MAE = 0.62 Mg/ha), GBM (R 2 = 0.88, MAE = 0.57 Mg/ha), and GBM (R 2 = 0.78, MAE = 0.66 Mg/ha), were the most accurate in predicting biomass yields of the Independence, Liberty, and Shawnee switchgrass cultivars, respectively. This is in agreement with similar studies that apply ML to multi-feature problems where traditional statistical methods are less applicable and datasets used were considered to be relatively small for ANNs. Consistent with previous studies on switchgrass, the most important predictors of biomass yield included average annual temperature, average growing season temperature, sum of the growing season precipitation, field slope, and elevation. This study helps pave the way for applying ML as a management tool for alternative bioenergy landscapes where understanding agronomic and environmental performance of a multifunctional cropping system seasonally and interannually at the sub-field scale is critical.

09 BIOMASS FUELS↗

Remote Sensing-Based Estimation of Advanced Perennial Grass Biomass Yields for Bioenergy

A sustainable bioeconomy would require growing high-yielding bioenergy crops on marginal agricultural areas with minimal inputs. To determine the cost competitiveness and environmental sustainability of such production systems, reliably estimating biomass yield is critical. However, because marginal areas are often small and spread across the landscape, yield estimation using traditional approaches is costly and time-consuming. This paper demonstrates the (1) initial investigation of optical remote sensing for predicting perennial bioenergy grass yields at harvest using a linear regression model with the green normalized difference vegetation index (GNDVI) derived from Sentinel-2 imagery and (2) evaluation of the model’s performance using data from five U.S. Midwest field sites. The linear regression model using midsummer GNDVI predicted yields at harvest with R2 as high as 0.879 and a mean absolute error and root mean squared error as low as 0.539 Mg/ha and 0.616 Mg/ha, respectively, except for the establishment year. Perennial bioenergy grass yields may be predicted 152 days before the harvest date on average, except for the establishment year. The green spectral band showed a greater contribution for predicting yields than the red band, which is indicative of increased chlorophyll content during the early growing season. Although additional testing is warranted, this study showed a great promise for a remote sensing approach for forecasting perennial bioenergy grass yields to support critical economic and logistical decisions of bioeconomy stakeholders.

09 BIOMASS FUELS↗

Data from: Leaching losses of dissolved organic carbon and nitrogen from agricultural soils in the upper US Midwest

Leaching losses of dissolved organic carbon (DOC) and nitrogen (DON) from agricultural systems are important to water quality and carbon and nutrient balances but are rarely reported; the few available studies suggest linkages to litter production (DOC) and nitrogen fertilization (DON). In this study we examine the leaching of DOC, DON, NO3-, and NH4+ from no-till corn (maize) and perennial bioenergy crops (switchgrass, miscanthus, native grasses, restored prairie, and poplar) grown between 2009 and 2016 in a replicated field experiment in the upper Midwest U.S. Leaching was estimated from concentrations in soil water and modeled drainage (percolation) rates. DOC leaching rates (kg ha-1 yr-1) and volume-weighted mean concentrations (mg L-1) among cropping systems averaged 15.4 and 4.6, respectively; N fertilization had no effect and poplar lost the most DOC (21.8 and 6.9, respectively). DON leaching rates (kg ha-1 yr-1) and volume-weighted mean concentrations (mg L-1) under corn (the most heavily N-fertilized crop) averaged 4.5 and 1.0, respectively, which was higher than perennial grasses (mean: 1.5 and 0.5, respectively) and poplar (1.6 and 0.5, respectively). NO3- comprised the majority of total N leaching in all systems (59-92%). Average NO3- leaching (kg N ha-1 yr-1) under corn (35.3) was higher than perennial grasses (5.9) and poplar (7.2). NH4+ concentrations in soil water from all cropping systems were relatively low (<0.07 mg N L-1). Perennial crops leached more NO3- in the first few years after planting, and markedly less after. Among the fertilized crops, the leached N represented 14-38% of the added N over the study period; poplar lost the greatest proportion (38%) and corn was intermediate (23%). Requiring only one third or less of the N fertilization compared to corn, perennial bioenergy crops can substantially reduce N leaching and consequent movement into aquifers and surface waters.

09 BIOMASS FUELS↗

Data from: Nitrification is a minor source of nitrous oxide (N2O) in an agricultural landscape and declines with increasing management intensity

The long-term contribution of nitrification to nitrous oxide (N2O) emissions from terrestrial ecosystems is poorly known and thus poorly constrained in biogeochemical models. Here, using Bayesian inference to couple 25 years of in situ N2O flux measurements with site-specific Michaelis-Menten kinetics of nitrification-derived N2O, we test the relative importance of nitrification-derived N2O across six cropped and unmanaged ecosystems along a management intensity gradient in the U.S. Midwest. We found that the maximum potential contribution from nitrification to in situ N2O fluxes was 13-17% in a conventionally fertilized annual cropping system, 27-42% in a low-input cover-cropped annual cropping system, and 52-63% in perennial systems including a late successional deciduous forest. Actual values are likely to be less than 10% of these values because of low N2O yields in cultured nitrifiers (typically 0.04 to 8% of NH3 oxidized) and competing sinks for available NH4+ in situ. Most nitrification-derived N2O was produced by ammonia oxidizing bacteria (AOB) rather than archaea (AOA), who appeared responsible for no more than 30% of nitrification-derived N2O production in all but one ecosystem. Although the proportion of nitrification-derived N2O production was lowest in annual cropping systems, these ecosystems nevertheless produced more nitrification-derived N2O (higher Vmax) than perennial and successional ecosystems. We conclude that nitrification is minor relative to other sources of N2O in all ecosystems examined.

54 ENVIRONMENTAL SCIENCES↗

Data from: Niche differentiation of bacterial versus archaeal soil nitrifiers induced by ammonium inhibition along a management gradient

Soil nitrification, mediated mainly by ammonia oxidizing archaea (AOA) and bacteria (AOB), converts ammonium (NH4+) to nitrite (NO2−) and thence nitrate (NO3−). To better understand ecological differences between AOA and AOB, we investigated the nitrification kinetics of AOA and AOB under eight replicated cropped and unmanaged ecosystems (including two fertilized natural systems) along a long-term management intensity gradient in the upper U.S. Midwest. For five of eight ecosystems, AOB but not AOA exhibited Haldane kinetics (inhibited by high NH4+ additions), especially in perennial and successional systems. In contrast, AOA predominantly exhibited Michaelis-Menten kinetics, suggesting greater resistance to high nitrogen inputs than AOB. These responses suggest the potential for NH4+-induced niche differentiation between AOA and AOB. Additionally, long-term fertilization significantly enhanced maximum nitrification rates (Vmax) in the early successional systems for both AOA and AOB, but not in the deciduous forest systems. This was likely due to pH suppression of nitrification in the acidic forest soils, corroborated by a positive correlation of Vmax with soil pH but not with amoA gene abundance. Results also demonstrated that soil nitrification potentials were relatively stable, as there were no seasonal differences. Overall, results suggest that (1) NH4+ inhibition of AOB but not AOA could be another factor contributing to niche differentiation between AOA and AOB in soil, and (2) nitrification by both AOA and AOB can be significantly promoted by long-term nitrogen inputs.

59 BASIC BIOLOGICAL SCIENCES↗

Cloud Top Phase Characterization of Extratropical Cyclones over the Northeast and Midwest United States: Results from IMPACTS

Cloud top phase (CTP) impacts cloud albedo and pathways for ice particle nucleation, growth, and fallout within extratropical cyclones. This study uses airborne lidar, radar, and Rapid Refresh analysis data to characterize CTP within extratropical cyclones as a function of cloud top temperature (CTT). During the 2020, 2022, and 2023 Investigation of Microphysics and Precipitation for Atlantic Coast-Threatening Snowstorms (IMPACTS) field campaign deployments, the Earth-Resources 2 (ER-2) aircraft flew 26 research flights over the Northeast and Midwest U.S. to sample the cloud tops of a variety of extratropical cyclones. A training dataset was developed to create probabilistic phase classifications based on Cloud Physics Lidar measurements of known ice and liquid clouds. These classifications were then used to quantify dominant CTP in the top 150 m of clouds sampled by the Cloud Physics Lidar in storms during IMPACTS. Case studies are presented illustrating examples of supercooled liquid water at cloud top at different CTT ranges (-3°C -20°C. Liquid-bearing cloud tops were found at CTTs as cold as -37ºC.

Troy J. Zaremba↗

Nature-based climate solutions can help mitigate the radiative forcing that follows deforestation

Widespread expansion of agriculture and forestry has altered the surface of the Earth, the composition of the atmosphere, and as a result, the climate. Here we quantify the radiative forcing caused by the historical deforestation of an ecoregion in the U.S. Upper Midwest and the adoption of eight nature-based climate solutions. We combined regional forest inventory data with over three decades of remote sensing and in situ data from a replicated land use change experiment. Deforestation of the region caused net global warming (1626 ± 44 µW m -2 ), mainly from the 76 % reduction of ecosystem carbon stocks, but also from the 84 % reduction of the soil methane sink and the 115 % increase in soil nitrous oxide emissions. The associated albedo increase offset 24 % of this greenhouse gas induced warming. For the adoption of nature-based climate solutions, we found that conservation agriculture can provide -39 to -76 ± 31 µW m -2 of climate mitigation over a 100-year time period while short/medium length forestry rotations can provide more at -296 to -881 ± 44 µW m -2 and natural forest regeneration can provide the most at -1555 ± 44 µW m -2 . As the impacts of climate change on nature and society intensify, consideration should be given to the climate mitigation, habitat, and ecosystem services that nature-based climate solutions can provide.

54 ENVIRONMENTAL SCIENCES↗