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NASA NTRS1983

Vertical ascending electrophoresis of cells with a minimal stabilizing medium

Vertical fractionation of a mixture of fixed horse and human red blood cells layered over a stabilizing support medium was done to give a valid comparison with proposed space experiments. In particular, the effects of sample thickness and concentration on zone migration rate were investigated. Electrophoretic mobilities of horse and human cells calculated from zone migration rates were compatible with those obtained by microelectrophoresis. Complete cell separation was observed when low power and effective cooling were employed.

Omenyi, S. N.↗

NASA NTRS1974

On the exposure history of the lunar regolith

The observed minimum and mean values of track densities in lunar grains are both found to exceed the values expected from exposure with redistribution by vertical mixing, and the percentage of the smaller grains showing very high track densities is too large by a factor of more than ten. Previous exposure of the material (in space before accretion onto the moon or in regions from which net migration has occurred) may be necessitated.

Gold, T.↗

NASA NTRS1985

Size and DNA distributions of electrophoretically separated cultured human kidney cells

Electrophoretic purification of purifying cultured cells according to function presumes that the size of cycle phase of a cell is not an overriding determinant of its electrophoretic velocity in an electrophoretic separator. The size distributions and DNA distributions of fractions of cells purified by density gradient electrophoresis were determined. No systematic dependence of electrophoretic migration upward in a density gradient column upon either size or DNA content were found. It was found that human leukemia cell populations, which are more uniform function and found in all phases of the cell cycle during exponential growth, separated on a vertical sensity gradient electrophoresis column according to their size, which is shown to be strictly cell cycle dependent.

Kunze, M. E.↗

NASA NTRS1990

The three-dimensional distribution of atmospheric heating during the GWE

The large-scale three-dimensional time-averaged distribution of heating and its temporal evolution as determined from the European Center for Medium-range Weather Forecasts (ECMWF) Global Weather Experiment (GWE) Level IIIb data set are examined. Global distributions of vertically averaged heating for January, April, July, and October 1979 are presented to illustrate the seasonal evolution of the planetary pattern. Horizontal distributions of heating and vertical profiles from various climatological regimes of the planetary circulation are given and provide insight into the four-dimensional structure of the thermal forcing of the atmosphere. Major features of the global distributions include heating in regions of deep moist convection over places such as South America. Seasonal meridional and zonal heating migration are discussed and it is noted that zonal migration is linked with planetary scale distribution of continents and oceans and the land-sea surface temperature distribution. Various degrees of heating in the troposphere and their consequences are investigated.

Schaack, Todd K.↗

NASA NTRS2006

Multistage Electrophoretic Separators

A multistage electrophoresis apparatus has been invented for use in the separation of cells, protein molecules, and other particles and solutes in concentrated aqueous solutions and suspensions. The design exploits free electrophoresis but overcomes the deficiencies of prior free-electrophoretic separators by incorporating a combination of published advances in mathematical modeling of convection, sedimentation, electro-osmotic flow, and the sedimentation and aggregation of droplets. In comparison with other electrophoretic separators, these apparatuses are easier to use and are better suited to separation in relatively large quantities characterized in the art as preparative (in contradistinction to smaller quantities characterized in the art as analytical). In a multistage electrophoretic separator according to the invention, an applied vertical steady electric field draws the electrically charged particles of interest from within a cuvette to within a collection cavity that has been moved into position of the cuvette. There are multiple collection cavities arranged in a circle; each is aligned with the cuvette for a prescribed short time. The multistage, short-migration-path character of the invention solves, possibly for the first time, the fluid-instability problems associated with free electrophoresis. The figure shows a prototype multistage electrophoretic separator that includes four sample stations and five collection stages per sample. At each sample station, an aqueous solution or suspension containing charged species to be separated is loaded into a cuvette, which is machined into a top plate. The apparatus includes a lower plate, into which 20 collection cavities have been milled. Each cavity is filled with an electrophoresis buffer solution. For the collection of an electrophoretic fraction, the lower plate is rotated to move a designated collection cavity into alignment with the opening of the cuvette. An electric field is then applied between a non-gassing electrode in the collection cavity and an electrolyte compartment, which is separated from the cuvette by a semipermeable membrane. The electrolyte is refreshed by circulation by use of a peristaltic pump. In subsequent steps, the lower plate is rotated to collect other electrophoretic fractions. Later, the collected fractions are removed from the collection cavities through ports that have threaded plugs. The base of the apparatus contains power supplies and a computer interface. The design includes provisions for monitoring and feedback control of cavity position, electric field, and temperature. The operation of the apparatus can easily be automated, as demonstrated by use of software that has already been written for this purpose.

Thomas, Nathan↗

NASA NTRS2017

Observations and Modeling of Thermal Structure in the Lower Atmosphere and the Upward Propagation of Tides into the Thermosphere

Thermal tides are the atmospheric response to diurnally varying thermal forcing resulting from radiative and convective heat transfer from the surface and from aerosol and gaseous heating within the atmosphere. Tides include sun-synchronous (migrating) waves driven in response to solar heating and additional non-migrating waves resulting from longitudinal variations in the distributions of topography, dust aerosol and water ice clouds. The systematic spatial mapping of temperature over 5 Mars years by the Mars Climate Sounder (MCS) has yielded a well-defined climatology of seasonally-varying temperature structures in the lower atmosphere, from 5 to ~80 km. Tide theory and Mars global circulation model (MGCM) simulations are a fruitful framework for relating temperature observations to thermal forcing by aerosol fields [1]. The analysis of density and temperature fields derived from MAVEN IUVS and NGIMS observations have revealed the presence of predominantly zonal wave 2 and 3 features at altitudes of 100-170 km that are almost certainly non-migrating tides propagating upward from the lower atmosphere [2,3]. In this presentation we will use the MCS climatology and MGCM simulations to relate the density variations seen by MAVEN with the seasonally varying tide activity in the lower atmosphere. Large amplitude perturbations in density are most sensitive to the tide components with the longest vertical wavelengths in temperature, which are well resolved in MCS observations.

Mars Climate Sounder (MCS)↗

NASA NTRS2014

Future Antarctic Bed Topography and Its Implications for Ice Sheet Dynamics

The Antarctic bedrock is evolving as the solid Earth responds to the past and ongoing evolution of the ice sheet. A recently improved ice loading history suggests that the Antarctic Ice Sheet (AIS) has generally been losing its mass since the Last Glacial Maximum. In a sustained warming climate, the AIS is predicted to retreat at a greater pace, primarily via melting beneath the ice shelves.We employ the glacial isostatic adjustment (GIA) capability of the Ice Sheet System Model (ISSM) to combine these past and future ice loadings and provide the new solid Earth computations for the AIS.We find that past loading is relatively less important than future loading for the evolution of the future bed topography. Our computations predict that the West Antarctic Ice Sheet (WAIS) may uplift by a few meters and a few tens of meters at years AD 2100 and 2500, respectively, and that the East Antarctic Ice Sheet is likely to remain unchanged or subside minimally except around the Amery Ice Shelf. The Amundsen Sea Sector in particular is predicted to rise at the greatest rate; one hundred years of ice evolution in this region, for example, predicts that the coastline of Pine Island Bay will approach roughly 45mmyr−1 in viscoelastic vertical motion. Of particular importance, we systematically demonstrate that the effect of a pervasive and large GIA uplift in the WAIS is generally associated with the flattening of reverse bed slope, reduction of local sea depth, and thus the extension of grounding line (GL) towards the continental shelf. Using the 3-D higher-order ice flow capability of ISSM, such a migration of GL is shown to inhibit the ice flow. This negative feedback between the ice sheet and the solid Earth may promote stability in marine portions of the ice sheet in the future.

ice dynamics↗

NASA NTRS1983

A potential-energy scaling model to simulate the initial stages of thin-film growth

A solid on solid (SOS) Monte Carlo computer simulation employing a potential energy scaling technique was used to model the initial stages of thin film growth. The model monitors variations in the vertical interaction potential that occur due to the arrival or departure of selected adatoms or impurities at all sites in the 400 sq. ft. array. Boltzmann ordered statistics are used to simulate fluctuations in vibrational energy at each site in the array, and the resulting site energy is compared with threshold levels of possible atomic events. In addition to adsorption, desorption, and surface migration, adatom incorporation and diffusion of a substrate atom to the surface are also included. The lateral interaction of nearest, second nearest, and third nearest neighbors is also considered. A series of computer experiments are conducted to illustrate the behavior of the model.

Heinbockel, J. H.↗

NASA NTRS1993

CO2 lidar observations of Mount Pinatubo debris: FIRE 2 and longer-term measurements

The volcanic debris in the stratosphere from the June 1991 eruption of Mt. Pinatubo first appeared over the NOAA Wave Propagation Laboratory (WPL) field site near Boulder, Colorado (40.15 N, 105.23 W), in July of 1991. The presence of the Pinatubo cloud has allowed us to characterize both the tropospheric and stratospheric aerosol backscatter using the NOAA/WPL CO2 Doppler lidar. The lidar has measured vertical backscatter profiles at lambda = 10.59 mu m for over a decade. Analysis of this dense set of profiles reveals the effects of atmospheric and microphysical processes during the buildup and decay of Mt. Pinatubo's clouds. Further information on the NOAA lidar, specifically calibrations using a hard target, can be found in Post and Cupp (1990). We present results of those measurements for June 15, 1991, through December 31, 1992. During that period of longer-term measurements, WPL took part in FIRE II (First ISCCP (International Satellite Cloud Climatology Project) Regional Experiment II), from November 12 through December 8, 1991, measuring vertical backscatter profiles almost daily. One of the mechanisms for purging stratospheric aerosols is tropopause folding, which occurs in cold-core extratropical cyclones. Tropospheric mass loading occurs during folding events which can substantially increase the amount of ice nuclei in the upper troposphere, and may affect the formation of cirrus in that region. Spring and fall are prominent times for tropopause folding events because of the migration of the subtropical and polar jet streams during the transition seasons. Sassen has suggested that the volcanic aerosols from Pinatubo played a role in the formation of cirrus during FIRE II, particularly during a period of moist subtropical flow on December 5-6, 1991.

Levinson, David H.↗

NASA NTRS1990

Rapid, bilateral changes in growth rate and curvature during gravitropism of cucumber hypocotyls: implications for mechanism of growth control

The growth response of etiolated cucumber (Cucumis sativus L.) hypocotyls to gravitropic stimulation was examined by means of time-lapse photography and high-resolution analysis of surface expansion and curvature. In comparison with video analysis, the technique described here has five- to 20-fold better resolution; moreover, the mathematical fitting method (cubic splines) allows direct estimation of local and integrated curvature. After switching seedlings from a vertical to horizontal position, both upper and lower surfaces of the stem reacted after a lag of about 11 min with a two- to three-fold increase in surface expansion rate on the lower side and a cessation of expansion, or slight compression, on the upper surface. This growth asymmetry was initiated simultaneously along the length of the hypocotyl, on both upper and lower surfaces, and did not migrate basipetally from the apex. Later stages in the gravitropic response involved a complex reversal of the growth asymmetry, with the net result being a basipetal migration of the curved region. This secondary growth reversal may reflect oscillatory and/or self-regulatory behaviour of growing cells. With some qualifications, the kinetics and pattern of growth response are consistent with a mechanism involving hormone redistribution, although they do not prove such a mechanism. The growth kinetics require a growth mechanism which can be stimulated by two- to three-fold or completely inhibited within a few minutes.

Non-NASA Center↗

NASA NTRS1990

Age constraints for the present fault configuration in the Imperial Valley, California: Evidence for northwestward propagation of the Gulf of California rift system

Releveling and other geophysical data for the Imperial Valley of southern California suggest the northern section of the Imperial-Brawley fault system, which includes the Mesquite Basin and Brawley Seismic Zone, is much younger than the 4 to 5 million year age of the valley itself. A minimum age of 3000 years is calculated for the northern segment of the Imperial fault from correlations between surface topography and geodetically observed seismic/interseismic vertical movements. Calculations of a maximum age of 80,000 years is based upon displacements in the crystalline basement along the Imperial fault, inferred from seismic refraction surveys. This young age supports recent interpretations of heat flow measurements, which also suggest that the current patterns of seismicity and faults in the Imperial Valley are not long lived. The current fault geometry and basement morphology suggest northwestward growth of the Imperial fault and migration of the Brawley Seismic Zone. It is suggested that this migration is a manifestation of the propagation of the Gulf of California rift system into the North American continent.

Larsen, Shawn↗

NASA NTRS1991

Age constraints for the present fault configuration in the Imperial Valley, California - Evidence for northwestward propagation of the Gulf of California rift system

Larsen, Shawn↗

NASA NTRS1996

Planetesimal Formation in the Outer Solar Nebula

A numerical investigation of the orbital trajectories of individual particles in the turbulent outer solar nebula has been performed. The (spherical) particle consists of an unchanging mm-sized 'dust' core surrounded by an H2O ice mantle; the density of both core and mantle is 0.5 g/cm(exp 3). The simulations include the effects of H2O condensation from the gas phase, H2O sublimation from the particle surface, and collisional growth via particle collisions with a background distribution of small H2O grains. The model nebula is an azimuthally symmetric minimum-mass nebula of solar composition with a vertical (and radial) temperature gradient. Particle evolution follows a pattern. A particle starting out in a cool region grows via condensation and collisional accretion until it is large enough (decimeter- to meter-sized) to decouple somewhat from the turbulence. (This growth occurs on a timescale of several thousand years at 10 AU; at 30 AU, the timescale is approx. 104 years.) The particle then moves rapidly inward toward the sun due to secular gas drag forces, sublimates much of its icy mantle, and slows its inward migration as it gets caught up in the turbulence again (due to its now-smaller size) at the 'sublimation boundary,' where the ambient gas temperature is approx. 150 K. Such a process could, on a short timescale (i.e., a timescale much shorter than the nebular gas lifetime of approx. 106 yr), generate a population of decimeter- to meter-sized bodies which would then collisionally accrete to form planetesimals.

Supulver, K. D.↗

NASA NTRS1989

Thermocapillary migration of liquid droplets in a temperature gradient in a density matched system

An experimental investigation of thermocapillary flow in droplets of a vegetable oil (partially hydrogenated soybean oil) immersed in silicone oil was conducted in a test cell with a heated top wall and a cooled bottom wall. The liquids are nearly immiscible and have equal densities at a temperature below the room temperature, thus providing a simulation of low-gravity conditions by reducing the buoyancy forces. The interfacial tension between the two oils was measured in the temperature range 20 to 50 C using a capillary tube and (d sigma)/(d T) was determined to be negative. Droplets ranging in sizes from 3 mm to 1 cm diameter were injected into the silicone oil. The vertical temperature profile in the bulk liquid (silicone oil) produces temperature variations along the interface which induce variations in the interfacial tension. The flow inside the droplet driven by the resulting interfacial shear stresses was observed using a laser light-sheet flow visualization technique. The flow direction is consistent with the sign of (d sigma)/(d T). The observed maximum surface velocities are compared to the theoretical predictions of Young et al. (1959).

Rashidnia, N.↗

NASA NTRS1991

Thermocapillary migration of liquid droplets in a temperature gradient in a density matched system

Rashidnia, N.↗

NASA NTRS1991

Factors controlling the structures of magma chambers in basaltic volcanoes

The depths, vertical extents, and lateral extents of magma chambers and their formation are discussed. The depth to the center of a magma chamber is most probably determined by the density structure of the lithosphere; this process is explained. It is commonly assumed that magma chambers grow until the stress on the roof, floor, and side-wall boundaries exceed the strength of the wall rocks. Attempts to grow further lead to dike propagation events which reduce the stresses below the critical values of rock failure. The tensile or compressive failure of the walls is discussed with respect to magma migration. The later growth of magma chambers is accomplished by lateral dike injection into the country rocks. The factors controlling the patterns of growth and cooling of such dikes are briefly mentioned.

Wilson, L.↗

NASA NTRS2009

Five-Year (2004-2009)Observations of Upper Tropospheric Water Vapor and Cloud Ice from MLS and Comparisons with GEOS-5 Analyses

This paper gives an overview of August 2004 through July 2009 upper tropospheric (UT) water vapor (H2O) and ice water content (IWC) from the Aura Microwave Limb Sounder (MLS) and comparisons with outputs from the NASA Goddard Earth Observing System Version 5 (GEOS-5) data assimilation system. Both MLS and GEOS-5 show that high values of H2O and IWC at 215 to 147 hPa are associated with areas of deep convection. They exhibit good (within approximately 15%) agreement in IWC at these altitudes, but GEOS-5 H2O is approximately 50% (215 hPa) to approximately 30% (147 hPa) larger than MLS, possibility due to its higher temperatures at these altitudes. GOES-5 produces a weaker intertropical convergence zone than MLS, while a seasonally-migrating band of tropical deep convection is clearly evident in both the MLS and GEOS-5 UT H2O and IWC. MLS and GEOS-5 both show spatial anti-correlation between IWC and H2O at 100 hPa, where less H2O is associated with low temperatures in regions of tropical convection. At 100 hPa, GEOS-5 produces 50% less IWC and 15% less H2O in the tropics, and approximately 20% more H2O in the extra-tropics, than does MLS. Behavior of the 100 hPa H2O, which exhibits a quasi-biennial oscillation, appears consistent with it being controlled by temperature. The seasonal cycle in the vertical transport of tropical mean H2O from approximately 147 hPa to approximately 10 hPa appears much stronger in MLS than in GEOS-5. The UT IWC and H2O interannual variations, from both MLS and GEOS-5, show clear imprints of the El Nino-Southern Oscillation.

Jiang, Jonathan H.↗

NASA NTRS1971

Plate convergence, transcurrent faults and internal deformation adjacent to Southeast Asia and the western Pacific

A model for oblique convergence between plates of lithosphere is proposed in which at least a fraction of slip parallel to the plate margin results in transcurrent movements on a nearly vertical fault which is located on the continental side of a zone of plate consumption. In an extreme case of complete decoupling only the component of slip normal to the plate margin can be inferred from underthrusting. Recent movements in the western Sunda region provide the most convincing evidence for decoupling of slip, which in this region is thought to be oblique to the plate margin. A speculative model for convergence along the margins of the Philippine Sea is constructed from an inferred direction of oblique slip in the Philippine region. This model requires that the triple point formed by the junction of the Japanese and Izu-Bonin trenches and the Nankai trough migrate along the Sagami trough.

Fitch, T. J.↗