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DOE OSTI2024

MultiGreen: A multiplexing architecture for GreenGate cloning

Genetic modification of plants fundamentally relies upon customized vector designs. The ever-increasing complexity of transgenic constructs has led to increased adoption of modular cloning systems for their ease of use, cost effectiveness, and rapid prototyping. GreenGate is a modular cloning system catered specifically to designing bespoke, single transcriptional unit vectors for plant transformation—which is also its greatest flaw. MultiGreen seeks to address GreenGate’s limitations while maintaining the syntax of the original GreenGate kit. The primary limitations MultiGreen addresses are 1) multiplexing in series, 2) multiplexing in parallel, and 3) repeated cycling of transcriptional unit assembly through binary intermediates. MultiGreen efficiently concatenates bespoke transcriptional units using an additional suite of level 1acceptor vectors which serve as an assembly point for individual transcriptional units prior to final, level 2, condensation of multiple transcriptional units. Assembly with MultiGreen level 1 vectors scales at a maximal rate of 2*⌈ log 6 n ⌉+3 days per assembly, where n represents the number of transcriptional units. Further, MultiGreen level 1 acceptor vectors are binary vectors and can be used directly for plant transformation to further maximize prototyping speed. MultiGreen is a 1:1 expansion of the original GreenGate architecture’s grammar and has been demonstrated to efficiently assemble plasmids with multiple transcriptional units. MultiGreen has been validated by using a truncated violacein operon from Chromobacterium violaceum in bacteria and by deconstructing the RUBY reporter for in planta functional validation. MultiGreen currently supports many of our in-house multi transcriptional unit assemblies and will be a valuable strategy for more complex cloning projects.

Science & Technology - Other Topics↗
DOE OSTI2023

Thermodynamic analysis of a two-stage binary-fluid ejector heat pump water heater

Ejector heat pump water heaters (EHPWHs) could significantly increase the thermal efficiency of domestic water heating and reduce greenhouse gas emissions. This study addresses two major technical barriers to using EHPWHs in domestic water heating—low heating cycle coefficient of performance (COP) and low condensation temperatures—using binary fluid pairs as a working fluid in a two-stage ejector system. A comprehensive, geometry-free model of binary-fluid ejectors was built and validated to predict the entrainment ratios of binary-fluid ejectors. A thermodynamic model of a two-stage binary-fluid ejector EHPWH was built to predict the heating cycle COP of EHPWHs. The performance of the EHPWH was theoretically evaluated using HFE7000 and Novec649 as primary fluids and R600 and R1234ze(Z) as secondary fluids. HFE7000/R600 gave the highest heating cycle COP (i.e., 1.356) in producing domestic hot water at 60.0 °C. An optimum evaporation temperature of the primary fluid was identified for the maximum effective entrainment ratio and effective pressure lift ratio of two-stage ejectors. Here, the effective entrainment ratio of the two-stage ejector dominated the heating cycle COP of the EHPWH. Primary fluids with lower latent heats of evaporation and/or secondary fluids with higher latent heats of evaporation yielded higher heating cycle COPs of EHPWHs.

42 ENGINEERING↗
DOE OSTI2021

The K2 M67 Study: Precise Mass for a Turnoff Star in the Old Open Cluster M67

We present a study of the bright detached eclipsing main-sequence binary WOCS 11028 (Sanders 617) in the open cluster M67. Although the binary has only one eclipse per orbital cycle, we show that the masses of the stars can be derived very precisely thanks to a strong constraint on the orbital inclination: M {sub A} = 1.222 ± 0.006M {sub ⊙} and M {sub B} = 0.909 ± 0.004M {sub ⊙}. We use a spectral energy distribution fitting method to derive characteristics of the component stars in lieu of the precise radii that would normally be derived from a doubly eclipsing binary. The deconvolution of the SEDs reveals that the brighter component of the binary is at the faint turnoff point for the cluster—a distinct evolutionary point that occurs after the convective core has been established and while the star is in the middle of its movement toward lower surface temperatures, before the so-called hook at the end of the main sequence. The measurements are in distinct disagreement with evolution models at solar metallicity: higher metal abundances are needed to reproduce the characteristics of WOCS 11028 A. We discuss the changes to model physics that are likely to be needed to address the discrepancies. The clearest conclusions are that diffusion is probably necessary to reconcile spectroscopic abundances of M67 stars with the need for higher metallicity models and that reduced strength convective overshooting is occurring for stars at the turnoff. At super-solar bulk metallicity, various indicators agree on a cluster age between about 3.5 and 4.0 Gyr.

36 MATERIALS SCIENCE↗
DOE OSTI2014

THE KOZAI-LIDOV MECHANISM IN HYDRODYNAMICAL DISKS

We use three-dimensional hydrodynamical simulations to show that a highly misaligned accretion disk around one component of a binary system can exhibit global Kozai-Lidov cycles, where the inclination and eccentricity of the disk are interchanged periodically. This has important implications for accreting systems on all scales, for example, the formation of planets and satellites in circumstellar and circumplanetary disks, outbursts in X-ray binary systems, and accretion onto supermassive black holes.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

TIC 278956474: Two Close Binaries in One Young Quadruple System Identified by TESS

We have identified a quadruple system with two close eclipsing binaries in Transiting Exoplanet Survey Satellite (TESS) data. The object is unresolved in Gaia and appears as a single source at parallax 1.08 ± 0.01 mas. Both binaries have observable primary and secondary eclipses and were monitored throughout TESS Cycle 1 (sectors 1–13), falling within the TESS Continuous Viewing Zone. In one eclipsing binary (P = 5.488 days), the smaller star is completely occluded by the larger star during the secondary eclipse; in the other (P = 5.674 days) both eclipses are grazing. Using these data, spectroscopy, speckle photometry, spectral energy distribution analysis, and evolutionary stellar tracks, we have constrained the masses and radii of the four stars in the two eclipsing binaries. The Li i equivalent width indicates an age of 10–50 Myr and, with an outer period of 858{sub −5}{sup +7} days, our analysis indicates this is one of the most compact young 2 + 2 quadruple systems known.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

Hydrogen-Bonding Interactions in Hybrid Aqueous/Nonaqueous Electrolytes Enable Low-Cost and Long-Lifespan Sodium-Ion Storage

Although “water-in-salt” electrolytes have opened a new pathway to expand the electrochemical stability window of aqueous electrolytes, the electrode instability and irreversible proton co-insertion caused by aqueous media still hinder the practical application, even when using exotic fluorinated salts. In this study, an accessible hybrid electrolyte class based on common sodium salts is proposed, and crucially an ethanol-rich media is introduced to achieve highly stable Na-ion electrochemistry. Here, ethanol exerts a strong hydrogen-bonding effect on water, simultaneously expanding the electrochemical stability window of the hybridized electrolyte to 2.5 V, restricting degradation activities, reducing transition metal dissolution from the cathode material, and improving electrolyte–electrode wettability. The binary ethanol–water solvent enables the impressive cycling of sodium-ion batteries based on perchlorate, chloride, and acetate electrolyte salts. Notably, a Na 0.44 MnO 2 electrode exhibits both high capacity (81 mAh g –1 ) and a remarkably long cycle life >1000 cycles at 100 mA g –1 (a capacity decay rate per cycle of 0.024%) in a 1 M sodium acetate system. The Na 0.44 MnO 2 /Zn full cells also show excellent cycling stability and rate capability in a wide temperature range. The gained understanding of the hydrogen-bonding interactions in the hybridized electrolyte can provide new battery chemistry guidelines in designing promising candidates for developing low-cost and long-lifespan batteries based on other (Li + , K + , Zn 2+ , Mg 2+ , and Al 3+ ) systems.

25 ENERGY STORAGE↗
DOE OSTI2021

NukeLM: Pre-Trained and Fine-Tuned Language Models for the Nuclear and Energy Domains

Natural language processing (NLP) tasks (text classification, named entity recognition, etc.) have seen amazing improvements over the last few years. This is due to models such as BERT that achieve deep knowledge transfer by using a large pre-trained model, then fine-tuning the model on specific tasks. The BERT architecture has shown even better performance on domain-specific tasks when the model is pre-trained using domain-relevant texts. Here, inspired by these recent advancements, we have developed NukeLM, a nuclear-domain BERT model pre-trained on 1.5 million abstracts from the DOE Office of Scientific and Technical Information (OSTI) database. This NukeLM model is then fine-tuned for the classification of research articles into either binary classes (related to the nuclear fuel cycle (NFC) or not) or multiple categories related to the subject of the article. We show that continued pre-training of a BERT-style architecture prior to fine-tuning results in greater performance in both article classification tasks. This information is critical for properly triaging manuscripts, a necessary task for better understanding citation networks that publish in the nuclear space and uncovering new areas of research in the nuclear (or nuclear relevant) domain.

96 KNOWLEDGE MANAGEMENT AND PRESERVATION↗
DOE OSTI2016

Chandra observations of eight sources discovered by INTEGRAL

We report on 0.3–10 keV observations with the Chandra X-ray Observatory of eight hard X-ray sources discovered within 8° of the Galactic plane by the International Gamma-ray Astrophysics Laboratory satellite. The short (∼5 ks) Chandra observations of the IGR source fields have yielded very likely identifications of X-ray counterparts for three of the IGR sources: IGR J14091–6108, IGR J18088–2741, and IGR J18381–0924. The first two have very hard spectra in the Chandra band that can be described by a power law with photon indices of Γ = 0.6 ± 0.4 and –0.7{sub −0.3}{sup +0.4}, respectively (90% confidence errors are given), and both have a unique near-IR counterpart consistent with the Chandra position. IGR J14091–6108 also displays a strong iron line and a relatively low X-ray luminosity, and we argue that the most likely source type is a cataclysmic variable (CV), although we do not completely rule out the possibility of a high mass X-ray binary. IGR J18088–2741 has an optical counterpart with a previously measured 6.84 hr periodicity, which may be the binary orbital period. We also detect five cycles of a possible 800–950 s period in the Chandra light curve, which may be the compact object spin period. We suggest that IGR J18088–2741 is also most likely a CV. For IGR J18381–0924, the spectrum is intrinsically softer with Γ=1.5{sub −0.4}{sup +0.5}, and it is moderately absorbed, N{sub H} = (4 ± 1) × 10{sup 22} cm{sup −2}. There are two near-IR sources consistent with the Chandra position, and they are both classified as galaxies, making it likely that IGR J18381–0924 is an active galactic nucleus. For the other five IGR sources, we provide lists of nearby Chandra sources, which may be used along with further observations to identify the correct counterparts, and we discuss the implications of the low inferred Chandra count rates for these five sources.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2017

Separating Stable Boron Isotopes in Uniflow Gas Centrifuges

A variant of a model of a uniflow gas centrifuge for separating a binary mixture of boron isotopes in the trifluoride form, taking account of the interaction of the rotating gas with a stationary cylindrical central body, is proposed. Expressions are obtained for the maximum admissible enrichment and separation factors and the optimal separative power as functions of the radius of the central body. It is shown that a cascade of uniflow gas centrifuges with more than 95% enrichment of boron trifluoride in terms of the {sup 10}B isotope in the requisite quantities for industry can be built in practice.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
DOE OSTI2014

Energy-dependent evolution in IC10 X-1: hard evidence for an extended corona and implications

We have analyzed a ∼130 ks XMM-Newton observation of the dynamically confirmed black hole + Wolf-Rayet (BH+WR) X-ray binary (XB) IC10 X-1, covering ∼1 orbital cycle. This system experiences periodic intensity dips every ∼35 hr. We find that energy-independent evolution is rejected at a >5σ level. The spectral and timing evolution of IC10 X-1 are best explained by a compact disk blackbody and an extended Comptonized component, where the thermal component is completely absorbed and the Comptonized component is partially covered during the dip. We consider three possibilities for the absorber: cold material in the outer accretion disk, as is well documented for Galactic neutron star (NS) XBs at high inclination; a stream of stellar wind that is enhanced by traveling through the L1 point; and a spherical wind. We estimated the corona radius (r {sub ADC}) for IC10 X-1 from the dip ingress to be ∼10{sup 6} km, assuming absorption from the outer disk, and found it to be consistent with the relation between r {sub ADC} and 1-30 keV luminosity observed in Galactic NS XBs that spans two orders of magnitude. For the other two scenarios, the corona would be larger. Prior BH mass (M {sub BH}) estimates range over 23-38 M {sub ☉}, depending on the inclination and WR mass. For disk absorption, the inclination, i, is likely to be ∼60-80°, with M {sub BH} ∼ 24-41 M {sub ☉}. Alternatively, the L1-enhanced wind requires i ∼ 80°, suggesting ∼24-33 M {sub ☉}. For a spherical absorber, i ∼ 40°, and M {sub BH} ∼ 50-65 M {sub ☉}.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2015

THE QUASI-ROCHE LOBE OVERFLOW STATE IN THE EVOLUTION OF CLOSE BINARY SYSTEMS CONTAINING A RADIO PULSAR

We study the evolution of close binary systems formed by a normal (solar composition), intermediate-mass-donor star together with a neutron star. We consider models including irradiation feedback and evaporation. These nonstandard ingredients deeply modify the mass-transfer stages of these binaries. While models that neglect irradiation feedback undergo continuous, long-standing mass-transfer episodes, models including these effects suffer a number of cycles of mass transfer and detachment. During mass transfer, the systems should reveal themselves as low-mass X-ray binaries (LMXBs), whereas when they are detached they behave as binary radio pulsars. We show that at these stages irradiated models are in a Roche lobe overflow (RLOF) state or in a quasi-RLOF state. Quasi-RLOF stars have radii slightly smaller than their Roche lobes. Remarkably, these conditions are attained for an orbital period as well as donor mass values in the range corresponding to a family of binary radio pulsars known as ''redbacks''. Thus, redback companions should be quasi-RLOF stars. We show that the characteristics of the redback system PSR J1723-2837 are accounted for by these models. In each mass-transfer cycle these systems should switch from LMXB to binary radio pulsar states with a timescale of approximately one million years. However, there is recent and fast growing evidence of systems switching on far shorter, human timescales. This should be related to instabilities in the accretion disk surrounding the neutron star and/or radio ejection, still to be included in the model having the quasi-RLOF state as a general condition.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2022

A KMnO 4 -Generated Colloidal Electrolyte for Redox Mediation and Anode Protection in a Li–Air Battery

The rechargeable lithium-oxygen (Li-O 2 ) battery has the highest theoretical specific energy density of any rechargeable batteries and could transform energy storage systems if a practical device could be attained. However, among numerous challenges, which are all interconnected, are polarization due to sluggish kinetics, low cycle life, small capacity, and slow rates. Here, in this study, we report on use of KMnO 4 to generate a colloidal electrolyte made up of MnO 2 nanoparticles. The resulting electrolyte provides a redox mediator for reducing the charge potential and lithium anode protection to increase cycle life. This electrolyte in combination with a stable binary transition metal dichalcogenide alloy, Nb 0.5 Ta 0.5 S 2 , as the cathode enables the operation of a Li-O 2 battery at a current density of 1 mA center dot cm -2 and specific capacity ranging from 1000 to 10000 mA center dot h center dot g -1 (corresponding to 0.1-1 mA center dot h center dot cm -2 ) in a dry air environment with a cycle life of up to 150. This colloidal electrolyte provides a robust approach for advancing Li-air batteries.

25 ENERGY STORAGE↗
DOE OSTI2021

TIC 168789840: A Sextuply Eclipsing Sextuple Star System

We report the discovery of a sextuply eclipsing sextuple star system from TESS data, TIC 168789840, also known as TYC 7037-89-1, the first known sextuple system consisting of three eclipsing binaries. The target was observed in Sectors 4 and 5 during Cycle 1, with lightcurves extracted from TESS Full Frame Image data. It was also previously observed by the WASP survey and ASAS-SN. The system consists of three gravitationally bound eclipsing binaries in a hierarchical structure of an inner quadruple system with an outer binary subsystem. Follow-up observations from several different observatories were conducted as a means of determining additional parameters. The system was resolved by speckle interferometry with a 0.″42 separation between the inner quadruple and outer binary, inferring an estimated outer period of ∼2 kyr. It was determined that the fainter of the two resolved components is an 8.217 day eclipsing binary, which orbits the inner quadruple that contains two eclipsing binaries with periods of 1.570 days and 1.306 days. Markov Chain Monte Carlo (MCMC) analysis of the stellar parameters has shown that the three binaries of TIC 168789840 are “triplets,” as each binary is quite similar to the others in terms of mass, radius, and T {sub eff}. As a consequence of its rare composition, structure, and orientation, this object can provide important new insight into the formation, dynamics, and evolution of multiple star systems. Future observations could reveal if the intermediate and outer orbital planes are all aligned with the planes of the three inner eclipsing binaries.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2021

Thermochemistry of sodium rare earth ternary fluorides, NaREF 4

Sodium rare earth fluorides, NaREF 4 (RE = rare earth), are used as luminescent materials for light emission and biomedical applications and are important compositions for extracting and separating RE elements. Solution calorimetric measurements of a series of β-structured NaREF 4 (Na 1.5 RE 1.5 F 6 ) phases with various RE elements determined their heats of formation. Though the lattice contracts from light to heavy RE elements, NaREF 4 compounds show more exothermic enthalpies of formation from binary components with the decrease of RE 3+ radii, contrary to behavior seen in most RE oxide ternary compounds. Furthermore, by constructing Born–Haber cycles, the different slopes of lines relating lattice energies to lattice parameters in binary and ternary fluorides appear to be the reason for this reverse trend, which can be associated with changes in the coordination number of RE cations. These trends and the metastability of sodium light RE fluorides not only reveal the key role of ionic radius in RE compound stability, but also are significant for the design and synthesis of new materials and motivate the more effective utilization of RE.

36 MATERIALS SCIENCE↗
DOE OSTI2017

Systematic Review of Life Cycle Greenhouse Gas Emissions from Geothermal Electricity

The primary goal of this work was to assess the magnitude and variability of published life cycle greenhouse gas (GHG) emission estimates for three types of geothermal electricity generation technologies: enhanced geothermal systems (EGS) binary, hydrothermal (HT) flash, and HT binary. These technologies were chosen to align the results of this report with technologies modeled in National Renewable Energy Laboratory's (NREL's) Regional Energy Deployment Systems (ReEDs) model. Although we did gather and screen life cycle assessment (LCA) literature on hybrid systems, dry steam, and two geothermal heating technologies, we did not analyze published GHG emission estimates for these technologies. In our systematic literature review of the LCA literature, we screened studies in two stages based on a variety of criteria adapted from NREL's Life Cycle Assessment (LCA) Harmonization study (Heath and Mann 2012). Of the more than 180 geothermal studies identified, only 29 successfully passed both screening stages and only 26 of these included estimates of life cycle GHG emissions. We found that the median estimate of life cycle GHG emissions (in grams of carbon dioxide equivalent per kilowatt-hour generated [g CO2eq/kWh]) reported by these studies are 32.0, 47.0, and 11.3 for EGS binary, HT flash, and HT binary, respectively (Figure ES-1). We also found that the total life cycle GHG emissions are dominated by different stages of the life cycle for different technologies. For example, the GHG emissions from HT flash plants are dominated by the operations phase owing to the flash cycle being open loop whereby carbon dioxide entrained in the geothermal fluids is released to the atmosphere. This is in contrast to binary plants (using either EGS or HT resources), whose GHG emissions predominantly originate in the construction phase, owing to its closed-loop process design. Finally, by comparing this review's literature-derived range of HT flash GHG emissions to data from currently operating geothermal plants, we found that emissions from operational plants exhibit more variability and the median of emissions from operational plants is twice the median of operational emissions reported by LCAs. Further investigation is warranted to better understand the cause of differences between published LCAs and estimates from operational plants and to develop LCA analytical approaches that can yield estimates closer to actual emissions.

15 GEOTHERMAL ENERGY↗
DOE OSTI2021

Experiments on the Capillary Condensation/Evaporation Hysteresis of Pure Fluids and Binary Mixtures in Cylindrical Nanopores

Here, a new experimental method, i.e., isochoric cooling/heating cycle measurement using differential scanning calorimeter (DSC), is applied to investigate the hysteretic phenomenon of pure components and mixtures confined in two different types of cylindrical nanopores (SBA-15 and KIT-6). The capillary condensation/evaporation hysteresis end point (T he ) for a specific system is determined by performing a series of measurements with different densities by increasing initial temperature and pressure. We confirm that for a pure component, T he is lower when confined in smaller nanopores, whereas T he for CH 4 /CO 2 and CH 4 /C 2 H 6 mixtures at the specific composition investigated are higher than that of pure CO 2 and C 2 H 6 , respectively, when confined in the same nanopores. To the best of our knowledge, this is the first time that T he of fluid mixture is experimentally studied, which further demonstrates that binary mixtures behave similarly to pure fluids when confined in nanoporous media. At low temperatures where hysteresis is observed, the shape as well as the duration of the exothermic peak (due to capillary condensation) and endothermic peak (due to capillary evaporation) indicate that capillary condensation, rather than capillary evaporation, takes place at/near a thermodynamic equilibrium. Within the accuracy of the measurements, we find that the total heat of capillary condensation is identical to that of capillary evaporation for all systems examined, regardless of the presence of hysteresis. By comparing the heat per unit volume among different systems, we also find that the heat per unit volume of the phase transition of confined pure fluid is lower than that of bulk, which further demonstrates that the molar density of confined pure liquid is less than that of bulk pure liquid at saturation.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗