Results for “binding modes” — Page 3

NASA NTRS1997

Ice velocity at the ice front of the Filchner-Ronne Ice Shelf, Antarctica, as observed with ERS interferometry

ERS images of the two ends of the ice front of the Filchner-Ronne ice shelf (Antarctica) were utilized interferometrically to study the deformation rate of the ice shelf in response to viscous creep. On the western bank of Berkner Island (BI), near Hemmen Ice Rise (HIR), a time series of ERS data acquired in February 1992, in both ascending and descending mode, and with a three-day time interval, were utilized to map the ice velocity in two dimensions. Finite-element ice-shelf flow simulations are compared with the ERS interferograms to interpret the ice motion in terms of the physical constraints on ice-shelf flow. The efforts to fit artificial interferograms generated with model velocity output suggest that the flow regime is strongly influenced by three processes. First, a void-creation process responsible for rifts at coastal margins tends to uncouple the ice shelf from the ice rise and neighboring coast of BI. Secondly, sea ice within the void space appears to act as a binding agent between discrete ice-shelf fragments, allowing rigid-body rotations of these fragments. Third, strain rates appear to be enhanced in a narrow zone adjacent to HIR, implying significant strain softening along the boundary. It is believed that synthetic aperture radar (SAR) interferogram/model intercomparison represents a powerful impetus towards the development of better, more physically realistic ice-shelf flow models.

Rignot, Eric↗

NASA NTRS1984

Fastener load tests and retention systems tests for cryogenic wind-tunnel models

A-286 stainless steel screws were tested to determine the tensile load capability and failure mode of various screw sizes and types at both cryogenic and room temperature. Additionally, five fastener retention systems were tested by using A-286 screws with specimens made from the primary metallic alloys that are currently used for cryogenic models. The locking system effectiveness was examined by simple no-load cycling to cryogenic temperatures (-275 F) as well as by dynamic and static loading at cryogenic temperatures. In general, most systems were found to be effective retention devices. There are some differences between the various devices with respect to ease of application, cleanup, and reuse. Results of tests at -275 F imply that the cold temperatures act to improve screw retention. The improved retention is probably the result of differential thermal contraction and/or increased friction (thread-binding effects). The data provided are useful in selecting screw sizes, types, and locking devices for model systems to be tested in cryogenic wind tunnels.

Wallace, J. W.↗

NASA NTRS2016

The IOAG Recommendations on Spacecraft Emergency Cross Support

In 2014, the Inter-agency Operations Advisory Group (IOAG) chartered a multi-agency team effort to study how to best handle spacecraft emergency as part of cross support among network assets. The original intent is to put in place for the first time a process and guidelines to make emergency support as part of a permanent cross-support capability between space agencies. From the perspective of space communications service providers, a few key issues, common to all agencies, concerning the Spacecraft Emergency Cross Support (SECS) have been explored. They are: context of emergency support, provision of emergency support under a cross-support agreement, provision of emergency support with no cross-support agreement, legal and liability issues, response time, support priority, services provided, sustaining emergency support capabilities, and charge for the support. The positions of the various participating agencies with respect to these issues have been collected, analyzed, and finally harmonized to form the recommended IOAG positions. A central challenge most communications service providers are facing is that since the spacecraft emergency is an unplanned critical event, it typically requires fast response to the emergency call, hence lacking an international standard process for the operational interfaces seems to exacerbate the difficulty in providing SECS. For reducing the response time, i.e. from the time of accepting a request for SECS to the readiness for support, it is recommended that a “cross-support emergency system” be established by IOAG member agencies. Along with it, the IOAG core services, just-in-time ground communications line, cross support service management (CSSM), and standard operations procedures (SOP) for operational interfaces form the basic foundation of the “cross support emergency system”. Of the above, new to the cross support conducted thus far in the IOAG community is the concept of the SOP specifically for the interfaces between the service provider and service user during the SECS. Use cases, salient features, and definition of the key operational activities/tasks relevant to the interfaces are addressed by the effort. Underpinning such a system is the availability of the RF license granted by the local authority, at national and/or regional level, for a given ground station to communicate with and track the spacecraft in emergency mode at the uplink and downlink frequencies assigned to that spacecraft. That means it is critical for the IOAG member agencies to obtain a priori all-band licenses (for the entire X-band or S-band) for some, if not all, of their ground stations that are most capable of or likely to provide SECS. It is also recommended that certain prior arrangements be made with the relevant local licensing authorities for a process that will allow expedited authorization to transmit/receive signals to/from the declared spacecraft over the declared ground stations specifically and solely for the emergency case. Our analysis of the SECS has also uncovered a few fundamental programmatic issues. Recognizing any IOAG positions reached on these issues do not necessarily lead to any binding authority, it is recommended that they, along with those key attributes of the cross support emergency system, be explicitly stated as multi-agency guidelines to guide the implementation and provision of the SECS. The paper will present the results of the working group on Spacecraft Emergency Cross Support and, in particular, its findings, products and recommendations. It will also identify the next steps of this work that may include the production of some international standards as an extension of this work, or the process to make this emergency system usable by other spacecraft operators beyond the space agencies.

Tai, Wallace S.↗

NASA NTRS2016

The IOAG Recommendations on Spacecraft Emergency Cross Support

In 2014, the Inter-agency Operations Advisory Group (IOAG) chartered a multi-agency team effort to study how to best handle spacecraft emergency as part of cross support among network assets. The original intent is to put in place for the first time a process and guidelines to make emergency support as part of a permanent cross-support capability between space agencies. From the perspective of space communications service providers, a few key issues, common to all agencies, concerning the Spacecraft Emergency Cross Support (SECS) have been explored. They are: context of emergency support, provision of emergency support under a cross-support agreement, provision of emergency support with no cross-support agreement, legal and liability issues, response time, support priority, services provided, sustaining emergency support capabilities, and charge for the support. The positions of the various participating agencies with respect to these issues have been collected, analyzed, and finally harmonized to form the recommended IOAG positions. A central challenge most communications service providers are facing is that since the spacecraft emergency is an unplanned critical event, it typically requires fast response to the emergency call, hence lacking an international standard process for the operational interfaces seems to exacerbate the difficulty in providing SECS. For reducing the response time, i.e. from the time of accepting a request for SECS to the readiness for support, it is recommended that a “cross-support emergency system” be established by IOAG member agencies. Along with it, the IOAG core services, just-in-time ground communications line, cross support service management (CSSM), and standard operations procedures (SOP) for operational interfaces form the basic foundation of the “cross support emergency system”. Of the above, new to the cross support conducted thus far in the IOAG community is the concept of the SOP specifically for the interfaces between the service provider and service user during the SECS. Use cases, salient features, and definition of the key operational activities/tasks relevant to the interfaces are addressed by the effort. Underpinning such a system is the availability of the RF license granted by the local authority, at national and/or regional level, for a given ground station to communicate with and track the spacecraft in emergency mode at the uplink and downlink frequencies assigned to that spacecraft. That means it is critical for the IOAG member agencies to obtain a priori all-band licenses (for the entire X-band or S-band) for some, if not all, of their ground stations that are most capable of or likely to provide SECS. It is also recommended that certain prior arrangements be made with the relevant local licensing authorities for a process that will allow expedited authorization to transmit/receive signals to/from the declared spacecraft over the declared ground stations specifically and solely for the emergency case. Our analysis of the SECS has also uncovered a few fundamental programmatic issues. Recognizing any IOAG positions reached on these issues do not necessarily lead to any binding authority, it is recommended that they, along with those key attributes of the cross support emergency system, be explicitly stated as multi-agency guidelines to guide the implementation and provision of the SECS. The paper will present the results of the working group on Spacecraft Emergency Cross Support and, in particular, its findings, products and recommendations. It will also identify the next steps of this work that may include the production of some international standards as an extension of this work, or the process to make this emergency system usable by other spacecraft operators beyond the space agencies.

Tai, Wallace S.↗

NASA NTRS2011

Cytometer on a Chip

A cytometer now under development exploits spatial sorting of sampled cells on a microarray chip followed by use of grating-coupled surface-plasmon-resonance imaging (GCSPRI) to detect the sorted cells. This cytometer on a chip is a prototype of contemplated future miniature cytometers that would be suitable for rapidly identifying pathogens and other cells of interest in both field and laboratory applications and that would be attractive as alternatives to conventional flow cytometers. The basic principle of operation of a conventional flow cytometer requires fluorescent labeling of sampled cells, stringent optical alignment of a laser beam with a narrow orifice, and flow of the cells through the orifice, which is subject to clogging. In contrast, the principle of operation of the present cytometer on a chip does not require fluorescent labeling of cells, stringent optical alignment, or flow through a narrow orifice. The basic principle of operation of the cytometer on a chip also reduces the complexity, mass, and power of the associated laser and detection systems, relative to those needed in conventional flow cytometry. Instead of making cells flow in single file through a narrow flow orifice for sequential interrogation as in conventional flow cytometry, a liquid containing suspended sampled cells is made to flow over the front surface of a microarray chip on which there are many capture spots. Each capture spot is coated with a thin (.50-nm) layer of gold that is, in turn, coated with antibodies that bind to cell-surface molecules characteristic of the cell species of interest. The multiplicity of capture spots makes it possible to perform rapid, massively parallel analysis of a large cell population. The binding of cells to each capture spot gives rise to a minute change in the index of refraction at the surface of the chip. This change in the index of refraction is what is sensed in GCSPRI, as described briefly below. The identities of the various species in a sample of cells is spatially encoded in the chip by the pattern of capture spots. The number of cells of a particular species is determined from the magnitude of the GCSPRI signal from that spot. GCSPRI as used here can be summarized as follows: The cytometer chip is fabricated with a diffraction grating on its front surface. The chip is illuminated with a light emitting diode (LED) from the front. By proper choice of grating parameters and of the wavelength and the angle of incidence of a laser beam, laser light can be made to be coupled into an electromagnetic mode that resonates with surface plasmons and thus couples light into surface plasmons. Coupling of light into a surface plasmon at a given location reduces the amount of incident light reflected from that location. A change in the index of refraction at the surface of a capture spot gives rise to a change in the resonance condition. Depending on the specific design, the change in the index of refraction could manifest itself as a brightening or darkening, a change in the wavelength needed to excite the plasmon at a given angle of incidence, or a change in the angle of incidence needed to excite the plasmon at a given wavelength. Whereas a multiwavelength laser system with multichannel detection would be needed to detect multiple species in conventional flow cytometry, it suffices to use an LED and a single detector channel in the GCSPRI approach: this contributes significantly to reductions in cost, complexity, size, mass, and power. GCSPRI cytometer chips could be made of plastic and could be mass-produced cheaply by use of molding and other methods adopted from the manufacture of digital video disks. These methods are amenable to a high degree of miniaturization: such additional features as fluidic channels, reaction chambers, and fluid-coupling ports could readily be incorporated into the chips, without incurring substantial additional costs.

Fernandez, Salvador M.↗

NASA NTRS2011

Cytometer on a Chip

A cytometer now under development exploits spatial sorting of sampled cells on a microarray chip followed by use of grating-coupled surface-plasmon-resonance imaging (GCSPRI) to detect the sorted cells. This cytometer on a chip is a prototype of contemplated future miniature cytometers that would be suitable for rapidly identifying pathogens and other cells of interest in both field and laboratory applications and that would be attractive as alternatives to conventional flow cytometers. The basic principle of operation of a conventional flow cytometer requires fluorescent labeling of sampled cells, stringent optical alignment of a laser beam with a narrow orifice, and flow of the cells through the orifice, which is subject to clogging. In contrast, the principle of operation of the present cytometer on a chip does not require fluorescent labeling of cells, stringent optical alignment, or flow through a narrow orifice. The basic principle of operation of the cytometer on a chip also reduces the complexity, mass, and power of the associated laser and detection systems, relative to those needed in conventional flow cytometry. Instead of making cells flow in single file through a narrow flow orifice for sequential interrogation as in conventional flow cytometry, a liquid containing suspended sampled cells is made to flow over the front surface of a microarray chip on which there are many capture spots. Each capture spot is coated with a thin (approximately 50-nm) layer of gold that is, in turn, coated with antibodies that bind to cell-surface molecules characteristic of one the cell species of interest. The multiplicity of capture spots makes it possible to perform rapid, massively parallel analysis of a large cell population. The binding of cells to each capture spot gives rise to a minute change in the index of refraction at the surface of the chip. This change in the index of refraction is what is sensed in GCSPRI, as described briefly below. The identities of the various species in a sample of cells is spatially encoded in the chip by the pattern of capture spots. The number of cells of a particular species is determined from the magnitude of the GCSPRI signal from that spot. GCSPRI as used here can be summarized as follows: The cytometer chip is fabricated with a diffraction grating on its front surface. The chip is illuminated with a light emitting diode (LED) from the front. By proper choice of grating parameters and of the wavelength and the angle of incidence of a laser beam, laser light can be made to be coupled into an electromagnetic mode that resonates with surface plasmons and thus couples light into surface plasmons. Coupling of light into a surface plasmon at a given location reduces the amount of incident light reflected from that location. A change in the index of refraction at the surface of a capture spot gives rise to a change in the resonance condition. Depending on the specific design, the change in the index of refraction could manifest itself as a brightening or darkening, a change in the wavelength needed to excite the plasmon at a given angle of incidence, or a change in the angle of incidence needed to excite the plasmon at a given wavelength. Whereas a multiwavelength laser system with multichannel detection would be needed to detect multiple species in conventional flow cytometry, it suffices to use an LED and a single detector channel in the GCSPRI approach: this contributes significantly to reductions in cost, complexity, size, mass, and power. GCSPRI cytometer chips could be made of plastic and could be mass-produced cheaply by use of molding and other methods adopted from the manufacture of digital video disks. These methods are amenable to a high degree of miniaturization: such additional features as fluidic channels, reaction chambers, and fluid-coupling ports could readily be incorporated into the chips, without incurring substantial additional costs.

Fernandez, Salvador M.↗

NASA NTRS1999

Molecular Mechanisms Regulating Muscle Fiber Composition Under Microgravity

The overall goal of this project is to reveal the molecular mechanisms underlying the selective and debilitating atrophy of specific skeletal muscle fiber types that accompanies sustained conditions of microgravity. Since little is currently known about the regulation of fiber-specific gene expression programs in mammalian muscle, elucidation of the basic mechanisms of fiber diversification is a necessary prerequisite to the generation of therapeutic strategies for attenuation of muscle atrophy on earth or in space. Vertebrate skeletal muscle development involves the fusion of undifferentiated mononucleated myoblasts to form multinucleated myofibers, with a concomitant activation of muscle-specific genes encoding proteins that form the force-generating contractile apparatus. The regulatory circuitry controlling skeletal muscle gene expression has been well studied in a number of vertebrate animal systems. The goal of this project has been to achieve a similar level of understanding of the mechanisms underlying the further specification of muscles into different fiber types, and the role played by innervation and physical activity in the maintenance and adaptation of different fiber phenotypes into adulthood. Our recent research on the genetic basis of fiber specificity has focused on the emergence of mature fiber types and have implicated a group of transcriptional regulatory proteins, known as E proteins, in the control of fiber specificity. The restriction of E proteins to selected muscle fiber types is an attractive hypothetical mechanism for the generation of muscle fiber-specific patterns of gene expression. To date our results support a model wherein different E proteins are selectively expressed in muscle cells to determine fiber-restricted gene expression. These studies are a first step to define the molecular mechanisms responsible for the shifts in fiber type under conditions of microgravity, and to determine the potential importance of E proteins as upstream targets for the effects of weightlessness. In the past year we have determined that the expression of E Proteins is restricted to specific fiber types by post-transcriptional mechanisms. By far, the most prevalent mechanism of cellular control for achieving post-transcriptional regulation of gene expression is selective proteolysis -through the ubiquitin -proteasome pathway. Steady-state levels of HEB message are similar in all fast and slow skeletal muscle fiber types, yet the protein is restricted to Type IIX fibers. HEB appears to be a nodal point for regulating fiber-specific transcription, as expression of the transcription factor is regulated at the post-transcriptional level. It is not clear at present whether the regulation is at the level of protein synthesis or degradation. We are now poised to evaluate the biological role of ubiquitination in fiber specific-gene expression by controlling the post-transcriptional expression of E Proteins. The use of metabolic labelling and pharmacological inhibitors of the ubiquitin pathway will be used to identify the mode of regulation of the Type IIX expression pattern. The potential role of specific kinases in effecting the restriction of HEB expression will be examined by using both inhibitors and activators. The results of these studies will provide the necessary information to evaluate the biological role of E proteins in controlling fiber type transitions, and in potentially attenuating the atrophic effects of microgravity conditions. We have also recently shown that ectopic expression of the HEB protein transactivates the Type IIX-specific skeletal a-actin reporter. The 218 bp skeletal a-actin promoter drives transgene expression solely in mature Type IIX fibers. A mouse also carrying the transgene MLCI/HEB (which ectopically expresses the E Protein HEB in Type IIB fibers) forces expression of the skeletal a-actin reporter gene in Type IIB fibers. We can now dissect the composition of this fiber-specific cis-element. The skeletal a-actin promoter is quite compact and has been extensively characterized in vitro for activity and binding factors. The single E box may act as a binding target of myogenic factor/HEB heterodimer to allow for IIX expression. The HEB transcription factor may recognize either the precise flanking sequences of the E Box, or perhaps interacting with other proteins bound nearby, and activating expression in Type IIX fibers. This E box will be both ablated, and alternatively, as ablation may well destroy any muscle-specific transcriptional activity, flanking sequences substituted with those surrounding the E box (El) of the myogenin promoter. Modification of fiber-specific transgene expression will be tested in transgenic mice. The results of these studies will provide basic information on the regulatory circuitry underlying fiber specificity, and will form the basis for building appropriate transgenic regulatory cassettes to effect fiber transitions in subsequent experimental manipulations on unweighted muscles.

Rosenthal, Nadia A.↗

NASA NTRS2013

Multi-User Space Link Extension (SLE) System

The Multi-User Space (MUS) Link Extension system, a software and data system, provides Space Link Extension (SLE) users with three space data transfer services in timely, complete, and offline modes as applicable according to standards defined by the Consultative Committee for Space Data Systems (CCSDS). MUS radically reduces the schedule, cost, and risk of implementing a new SLE user system, minimizes operating costs with a lights-out approach to SLE, and is designed to require no sustaining engineering expense during its lifetime unless changes in the CCSDS SLE standards, combined with new provider implementations, force changes. No software modification to MUS needs to be made to support a new mission. Any systems engineer with Linux experience can begin testing SLE user service instances with MUS starting from a personal computer (PC) within five days. For flight operators, MUS provides a familiar-looking Web page for entering SLE configuration data received from SLE. Operators can also use the Web page to back up a space mission's entire set of up to approximately 500 SLE service instances in less than five seconds, or to restore or transfer from another system the same amount of data from a MUS backup file in about the same amount of time. Missions operate each MUS SLE service instance independently by sending it MUS directives, which are legible, plain ASCII strings. MUS directives are usually (but not necessarily) sent through a TCP-IP (Transmission Control Protocol Internet Protocol) socket from a MOC (Mission Operations Center) or POCC (Payload Operations Control Center) system, under scripted control, during "lights-out" spacecraft operation. MUS permits the flight operations team to configure independently each of its data interfaces; not only commands and telemetry, but also MUS status messages to the MOC. Interfaces can use single- or multiple-client TCP/IP server sockets, TCP/IP client sockets, temporary disk files, the system log, or standard in, standard out, or standard error as applicable. By defining MUS templates in ASCII, the flight operations team can include any MUS system variable in telemetry or command headers or footers, and/or in status messages. Data fields can be arranged within messages in different sequences, according to the mission s needs. The only constraints imposed are on the format of MUS directive strings, and some bare minimum logical requirements that must be met in order for MUS to read the mission control center's spacecraft command inputs. The MUS system imposes no limits or constraints on the numbers and combinations of missions and SLE service instances that it will support simultaneously. At any time, flight operators may add, change, delete, bind, connect, or disconnect.

Perkins, Toby↗