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Bone growth and composition in weanling and mature rats exposed to chronic centrifugation

The primary objective of the study is to determine the effect of continuous exposure to hypergravity on the development and composition of weight-bearing bone. The experimental results are seen to suggest that many, if not all, of the changes observed in bone growth and composition derive from the retarded growth rate of the centrifuged rats. Both centrifuged weanling and mature rats exhibit a significant reduction in femur length and mass. The changes in femur size are more apparent in the weanlings since they are exposed to hypergravity during their most rapid phase of skeletal development. In addition to a slower growth rate, the body mass of the mature and weanling animals is reduced even further by the depletion of body fat. The rapid loss of body fat observed in rats and mice during centrifugation, it is found, can produce a prompt and significant rise in relative femur mass after two weeks of exposure. After adaptation to centrifugation, however, relative femur mass is similar to that of controls at four and eight weeks. At 18 weeks, the centrifuged rats again exhibit an increase in relative femur mass. It is thought that this increase in relative femur mass may be generated by the difference in fat deposition between the 1-G controls and the high-G rats.

Keil, L. C.↗

Response of rat body composition to simultaneous exercise and centrifugation at 3.14g

A study is described calling into question the hypothesis that an increased physical load during chronic centrifugation contributes to the body composition changes observed in centrifuged rats. Considering fat-free and fat-free dry masses of the total body and carcass, it is seen that centrifugation combined with either wheel running or restraint reduced these masses to approximately 85% of the respective 1-g values, that is, the same result with either sedentary rats or rats running several hundred meters per day. It is pointed out that if an effect of a centrifugation-induced load is present but hidden by opposing factors, an analysis of variance should reveal it as an interaction between acceleration and other variables; however, no such interactions are found here. Tables are included emphasizing the pervasive influence of chronic centrifugation after only 12 days exposure.

Pitts, G. C.↗

Distribution of fluids in the body of the centrifuged rat

The effects of exposure to an elevated g-level throughout the period of rapid growth is investigated in a comparison of a group of female Sprague-Dawley rats centrifuged as adults with other groups centrifuged for prolonged intervals starting shortly after weaning. The fluid-solid composition of total body, heart, liver, gut, skin, and muscle for both study groups is compared with that of a control group. None of the changes as a result of centrifugation were truly persistent. The only increases in mass associated with centrifugation and the only responses to centrifugation per se were observed in the skin values.

Pitts, G. C.↗

Scientific uses and technical implementation of a variable gravity centrifuge on Space Station Freedom

The potential need and science requirements for a centrifuge to be designed and flown on Space Station Freedom are discussed, with a focus on a design concept for a centrifuge developed at NASA Ames. Applications identified for the centrifuge include fundamental studies in which gravity is a variable under experimental control, the need to provide a 1-g control, attempts to discover the threshold value of gravitation force for psychological response, and an effort to determine the effects of intermittent hypergravity. Science requirements specify the largest possible diameter at approximately 2.5 m, gravity levels ranging from 0.01 to 2 g, a nominal ramp-up rate of 0.01 g/sec, and life support for plants and animals. Ground-based studies using rats and squirrel monkeys on small-diameter centrifuges have demonstrated that animals can adapt to centrifugation at gravity gradients higher than those normally used in ground-based hypergravity studies.

Johnson, C. C.↗

Effect of science laboratory centrifuge of space station environment

It is argued that it is essential to have a centrifuge operating during manned space station operations. Background information and a rationale for the research centrifuge are given. It is argued that we must provide a controlled acceleration environment for comparison with microgravity studies. The lack of control groups in previous studies throws into question whether the obseved effects were the result of microgravity or not. The centrifuge could be used to provide a 1-g environment to supply specimens free of launch effects for long-term studies. With the centrifuge, the specimens could be immediately transferred to microgravity without undergoing gradual acclimation. Also, the effects of artificial gravity on humans could be investigated. It is also argued that the presence of the centrifuge on the space station will not cause undo vibrations or other disturbing effects.

Searby, Nancy↗

Centrifugal acceleration of the polar wind

The effect of parallel ion acceleration associated with convection was first applied to energization of test particle polar ions by Cladis (1986). However, this effect is typically neglected in 'self-consistent' models of polar plasma outflow, apart from the fluid simulation by Swift (1990). Here we include approximations for this acceleration, which we broadly characterize as centrifugal in nature, in our time-dependent, semikinetic model of polar plasma outflow and describe the effects on the bulk parameter profiles and distribution functions of H+ and O+. For meridional convection across the pole the approximate parallel force along a polar magnetic field line may be written as F(sub cent, pole) = 1.5m(E(sub i))/B(sub i))squared (r(squared)/r(sup 3)(sub i)) where m is ion mass, r is geometric distance; and E(sub i), B(sub i) and r(sub i) refer to the electric and magnetic field magnitudes and geocentric distance at the ionosphere, respectively. For purely longitudinal convection along a constant L shell the parallel force is F(cent. long) = F(sub cent, pole)(1 - (r/(r(sub i)L))(sup 3/2)/(1 - 3r/(4 r(sub i)L))(sup 5/2). For high latitudes the difference between these two cases is relatively unimportant below approximately 5 R(sub E). We find that the steady state O+ bulk velocities and parallel temperatures strongly increase and decrease, respectively, with convection strength. In particular, the bulk velocities increase from near 0 km/s at 4000 km altitude to approximately 10 km/s at 5 R(sub E) geocentric distance for 50-mV/m ionospheric convection electric field. However, the centrifugal effect on the steady O+ density profiles depends on the exobase ion and electron temperatures: for low-base temperatures (T(sub i) = T(sub e) = 3000 K) the O+ density at high altitudes increases greatly with convection, while for higher base temperatures (T(sub i) = 5000 K, T(sub e) = 9000 K), the high-altitude O+ density decreases somewhat as convection is enhanced. The centrifugal force further has a pronounced effect on the escaping O+ flux, especially for cool exobase conditions; as referenced to the 4000-km altitude, the steady state O+ flux increases from 10(exp 5) ions/sq cm/s when the ionospheric convection field E(sub i) = 0 mV/m to approximately 10(exp 7) ions/sq cm/s when E(sub i) = 100 mV/m. The centrifugal effect also decreases the time scale for approach to steady-state. For example, in the plasma expansion for T(sub i) = T(sub e) = 3000 K, the O+ density at 7 R(sub E) reaches only 10(exp -7) of it final value approximately 1.5 hours after expansion onset for E(sub i) = 0. For meridional convection driven by E(sub i) = 50 mV/m, the density at the same time after initial injection is 30-50% of its asymptotic level. The centrifugal acceleration described here is a possible explanation for the large (up to approximately 10 km/s or more) o+ outflow velocities observed in the midlatitude polar magnetosphere with the Dynamics Explorer 1 and Akebono spacecraft.

Horwitz, J. L.↗

Mechanisms of Sensorimotor Adaptation to Centrifugation

We postulate that centripetal acceleration induced by centrifugation can be used as an inflight sensorimotor countermeasure to retain and/or promote appropriate crewmember responses to sustained changes in gravito-inertial force conditions. Active voluntary motion is required to promote vestibular system conditioning, and both visual and graviceptor sensory feedback are critical for evaluating internal representations of spatial orientation. The goal of our investigation is to use centrifugation to develop an analog to the conflicting visual/gravito-inertial force environment experienced during space flight, and to use voluntary head movements during centrifugation to study mechanisms of adaptation to altered gravity environments. We address the following two hypotheses: (1) Discordant canal-otolith feedback during head movements in a hypergravity tilted environment will cause a reorganization of the spatial processing required for multisensory integration and motor control, resulting in decreased postural stability upon return to normal gravity environment. (2) Adaptation to this "gravito-inertial tilt distortion" will result in a negative after-effect, and readaptation will be expressed by return of postural stability to baseline conditions. During the third year of our grant we concentrated on examining changes in balance control following 90-180 min of centrifugation at 1.4 9. We also began a control study in which we exposed subjects to 90 min of sustained roll tilt in a static (non-rotating) chair. This allowed us to examine adaptation to roll tilt without the hypergravity induced by centrifugation. To these ends, we addressed the question: Is gravity an internal calibration reference for postural control? The remainder of this report is limited to presenting preliminary findings from this study.

Paloski, W. H.↗

Development of a Feeder for Uninterrupted Centrifugation Studies

A specialized paste diet feeder was developed in support of a hypergravity (2G) centrifuge study. The centrifuge study was to be compared to a previously flown Russian Cosmos spaceflight so experimental parameters of the 14 day spaceflight had to be duplicated. In order to duplicate at hyper G an experiment that took place in weightlessness, all other conditions must be as identical as possible. Stopping the centrifuge to provide maintenance for the animals causes unacceptable changes in experimental research results. Thus the experimental protocol required the delivery of a designated amount of paste diet at regular intervals for a two week period without stopping the centrifuge. A centrifuge and a stationary control cage, each containing 10 laboratory rats, were fitted with feeders that were calibrated to provide 140 plus or minus 2g of paste diet every 6 hours. This paper describes development of the feeder design and results of its operation over the two week experiment. The design philosophy and details of the feeder system are provided with recommendations for future such devices.

Mulenburg, Gerald M.↗

Life Sciences Research in the Centrifuge Accommodation Module of the International Space Station

The Centrifuge Accommodation Module (CAM) will be the home of the fundamental biology research facilities on the International Space Station (ISS). These facilities are being built by the Biological Research Project (BRP), whose goal is to oversee development of a wide variety of habitats and host systems to support life sciences research on the ISS. The habitats and host systems are designed to provide life support for a variety of specimens including cells, bacteria, yeast, plants, fish, rodents, eggs (e.g., quail), and insects. Each habitat contains specimen chambers that allow for easy manipulation of specimens and alteration of sample numbers. All habitats are capable of sustaining life support for 90 days and have automated as well as full telescience capabilities for sending habitat parameters data to investigator homesite laboratories. The habitats provide all basic life support capabilities including temperature control, humidity monitoring and control, waste management, food, media and water delivery as well as adjustable lighting. All habitats will have either an internal centrifuge or are fitted to the 2.5-meter diameter centrifuge allowing for variable centrifugation up to 2 g. Specimen chambers are removable so that the specimens can be handled in the life sciences glovebox. Laboratory support equipment is provided for handling the specimens. This includes a compound and dissecting microscope with advanced video imaging, mass measuring devices, refrigerated centrifuge for processing biological samples, pH meter, fixation and complete cryogenic storage capabilities. The research capabilities provided by the fundamental biology facilities will allow for flexibility and efficiency for long term research on the International Space Station.

Dalton, Bonnie P.↗

Liquid solution centrifugation for safe, scalable, and efficient isotope separation

A general method of separating isotopes by centrifuging dissolved chemical compounds in a liquid is introduced. This technique can be applied to almost all elements and leads to large separation factors. The method has been demonstrated in several isotopic systems including Ca, Mo, O, and Li with single-stage selectivities of 1.046 to 1.067 per neutron mass difference (e.g., 1.43 in 40 Ca/ 48 Ca), which are beyond the capabilities of various conventional methods. Equations are derived to model the process, and the results agree with those of the experiments. The scalability of the technique has been demonstrated by a three-stage enrichment of 48 Ca with a total 40 Ca/ 48 Ca selectivity of 2.43, and the scalability is more broadly supported through analogies to gas centrifuge, whereby countercurrent centrifugation can further multiply the separation factor by 5 to 10 times per stage in a continuous process. Optimal centrifuge conditions and solutions can achieve both high-throughput and highly efficient isotope separation.

07 ISOTOPE AND RADIATION SOURCES↗

MCTrans++: a 0-D model for centrifugal mirrors

The centrifugal mirror confinement scheme incorporates supersonic rotation of a plasma into a magnetic mirror device. This concept has been shown experimentally to drastically decrease parallel losses and increase plasma stability as compared with prior axisymmetric mirrors. MCTrans++ is a dimensionless (0-D) scoping tool which rapidly models experimental operating points in the Centrifugal Mirror Fusion Experiment (CMFX) at the University of Maryland. In the low-collisionality regime, parallel losses can be modelled analytically. A confining potential is set up that is partially ambipolar and partially centrifugal. Due to the stabilizing effects of flow shear, the perpendicular losses can be modelled as classical. Radiation losses such as bremsstrahlung and cyclotron emission are taken into account. A neutrals model is included, and, in some circumstances, charge-exchange losses are found to exceed all other loss mechanisms. We use the SUNDIALS ARKODE library to solve the underlying equations of this model; the resulting software is suitable for scanning large parameter spaces, and can also be used to model time-dependent phenomena such as a capacitive discharge. MCTrans++ has been used to verify results from prior centrifugal mirrors, create an experimental plan for CMFX and find configurations for future reactor-scale fusion devices.

Schwartz, Nick R. (ORCID:0000000296846435)↗

Effect of chronic centrifugation on body composition in the rat.

Two groups of adult female rats were chronically centrifuged for 60 days (2.76 G, 4.15 G, controls at 1.00 G). Live weights of centrifugal rats decreased about 20 g (6%) per Delta 1 G above control. This weight loss comprised reductions in both body fat and fat-free body weight (FFBW) as determined by body-composition studies on eight rats per group killed at the end of centrifugation. Of nine components constituting the FFBW, only skeletal muscle, liver, and heart changed significantly in weight. Chemical composition showed reductions (compared with controls) in the fat fraction of most components and increases in the water fraction of liver and gut. Identical measurements were made on the remaining eight rats per group killed 43 days after return to 1 G. Neither centrifuged group had reached the control body-weight level at this time. No statistically significant effect of previous G level was found in any of the body-composition parameters. The possible involvment of physiological regulation was considered.

Pitts, G. C.↗

Effects of centrifugation on gonadal and adrenocortical steroids in rats

Many endocrine systems are sensitive to external changes in the environment. Both the pituitary adrenal and pituitary gonadal systems are affected by stress including centrifugation stress. The effect of centrifugation on the pituitary gonadal and pituitary adrenocortical systems was examined by measuring the gonadal and adrenal steroids in the plasma and brain following different duration and intensity of centrifugation stress in rats. Two studies were completed and the results are presented. The second study was carried out to describe the developmental changes of brain, plasma and testicular testosterone and dihydrotestosterone in Sprague Dawley rats so that the effect of centrifugation stress on the pituitary gonadal syatem could be better evaluated in future studies.

Kakihana, R.↗

Enhancing Centrifugal Separation With Electrophoresis

Separation of biological cells by coil-planet centrifuge enhanced by electrophoresis. By itself, coil-planet centrifuge offers relatively gentle method of separating cells under low centrifugal force in physiological medium that keeps cells alive. With addition of voltage gradient to separation column of centrifuge, separation still gentle but faster and more complete. Since separation apparatus contains no rotary seal, probability of leakage, contamination, corrosion, and short circuits reduced.

Herrmann, F. T.↗

Need, utilization, and configuration of a large, multi-G centrifuge on the Space Station

A large, multi-g centrifuge is required on the Space Station (1) to provide valid 1-g controls for the study of zero-g effects on animals and plants and to study readaptation to 1 g; (2) to store animals at 1 g prior to short-term zero-g experimentation; (3) to permit g-level threshold studies of gravity effects. These requirements can be met by a 13-ft-diam., center-mounted centrifuge, on which up to 48 modular habitats with animals (squirrel monkey, rat, mouse) and plants are attached. The advantages of locating this centrifuge with the vivarium, a common environmental control and life support system, a general-purpose work station and storage of food, water, and supplies in an attached short module, are elaborated. Servicing and operation of the centrifuge, as well as minimizing its impact on other Space Station functions are also considered.

Bonting, Sjoerd L.↗

Research centrifuge accommodations on Space Station Freedom

Life sciences research using plants and animals on the Space Station Freedom requires the ability to maintain live subjects in a safe and low stress environment for long durations at microgravity and at one g. The need for a centrifuge to achieve these accelerations is evident. Programmatic, technical, and cost considerations currently favor a 2.5 meter diameter centrifuge located either in the end cone of a Space Station Freedom node or in a separate module. A centrifuge facility could support a mix of rodent, plant, and small primate habitats. An automated cage extractor could be used to remove modular habitats in pairs without stopping the main rotor, minimizing the disruption to experiment protocols. The accommodation of such a centrifuge facility on the Space Station represents a significant demand on the crew time, power, data, volume, and logistics capability. It will contribute to a better understanding of the effects of space flight on humans, an understanding of plant growth in space for the eventual production of food, and an understanding of the role of gravity in biological processes.

Arno, Roger D.↗

Centrifuge facility conceptual system study. Volume 2: Facility systems and study summary

The Centrifuge Facility is a major element of the biological research facility for the implementation of NASA's Life Science Research Program on Space Station Freedom using nonhuman species (small primates, rodents, plants, insects, cell tissues, etc.). The Centrifuge Facility consists of a variable gravity Centrifuge to provide artificial gravity up to 2 earth G's' a Holding System to maintain specimens at microgravity levels, a Glovebox, and a Service Unit for servicing specimen chambers. The following subject areas are covered: (1) Holding System; (2) Centrifuge System; (3) Glovebox System; (4) Service System; and (5) system study summary.

Synnestvedt, Robert↗

Active unsteady aerodynamic suppression of rotating stall in an incompressible flow centrifugal compressor with vaned diffuser

A mathematical model is developed to analyze the suppression of rotating stall in an incompressible flow centrifugal compressor with a vaned diffuser, thereby addressing the important need for centrifugal compressor rotating stall and surge control. In this model, the precursor to to instability is a weak rotating potential velocity perturbation in the inlet flow field that eventually develops into a finite disturbance. To suppress the growth of this potential disturbance, a rotating control vortical velocity disturbance is introduced into the impeller inlet flow. The effectiveness of this control is analyzed by matching the perturbation pressure in the compressor inlet and exit flow fields with a model for the unsteady behavior of the compressor. To demonstrate instability control, this model is then used to predict the control effectiveness for centrifugal compressor geometries based on a low speed research centrifugal compressor. These results indicate that reductions of 10 to 15 percent in the mean inlet flow coefficient at instability are possible with control waveforms of half the magnitude of the total disturbance at the inlet.

Lawless, Patrick B.↗