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Design Variants of a Common Habitat for Moon and Mars Exploration

The Common Habitat is a long-duration habitat concept based on the Skylab II architecture that leverages a single, multi-destination design applicable to microgravity Mars transit, 1/6 g lunar surface, 3/8 g Mars surface, and 1 g Earth. A trade study for the Common Habitat will address vertical versus horizontal internal orientation and a crew size of four or eight crew. This has resulted in the creation of four variants of the Common Habitat: Four Crew Horizontal Configuration, Four Crew Vertical Configuration, Eight Crew Horizontal Configuration, and Eight Crew Vertical Configuration. Design guidelines that shaped the four configurations are discussed, including: mission duration, destinations/missions, pressure vessel, hatches and docking, subsystems and utilities, lander integration and offloading, and eight-crew extensibility. Functional capabilities for crew-related systems are also discussed, including: private habitation, meal preparation, meal consumption, medical operations, exercise, group socialization and recreation, human waste collection, hygiene, logistics, spacecraft monitoring and commanding, mission planning, robotics and teleoperation, scientific research, maintenance and fabrication, and EVA. Each of the four Common Habitat designs will be presented, with a deck-by-deck description of each workstation, crew station, or subsystem along with an assessment of its degree of compliance with the guidelines and functional capabilities. Finally, forward work will be identified that will down-select a single Common Habitat. This includes multiple analyses that will be performed on the four variants, a down-selection process, and design refinement goals for the selected variant.

Habitability↗

Internal Architecture of the Common Habitat

The core stage liquid oxygen tank of the Space Launch System can be manufactured as a habitat instead of as a propellant tank, with a common design such that it is equally suitable for use in 0g, 1/6g, 3/8g, 1g, or variable artificial gravity. This Common Habitat can be the central element of a human spaceflight architecture that encompasses the Moon, Mars, and other destinations within the inner solar system. The Common Habitat is specifically used within an alternative architecture study (that is not part of the current NASA baseline) as the core habitation element within a Lunar Basecamp, Mars Basecamp, and the Deep Space Exploration Vehicle. The Common Habitat internal architecture applies a design philosophy to separate crew functions according to deck. The lower deck is reserved for private functions. It includes eight private crew quarters and four waste and hygiene clusters – each with a private waste management compartment, private full body hygiene compartment, and foyer/clothes changing area. The mid deck is primarily allocated to mission-related working functions. It includes an exercise facility, fabrication / maintenance / repair facility, physical science laboratory (physics, geology, and astronomy), and life science laboratory (biology and human research). The mid deck also has four external hatches, clocked one every 90 degrees, centered on the vehicle vertical centerline. Each hatch has a 60-inch tall by 40-inch wide opening with the mid deck floor 16 inches below the bottom lip of the hatch opening. The upper deck is primarily allocated to group or social functions. It includes a large galley, wardroom with projector and display screen, plant growth chambers, bulk stowage, command and control station, medical facility, hygiene compartment, and vehicle subsystems. A Vertical Translation System, based in part on results from a GrabCAD public challenge, enables crew and equipment traverse between habitat decks in 0g, 1/6g, 3/8g, and 1g. A 40-inch by 60-inch vertical translation corridor ensures that anything that can fit through the external hatches can also be moved from deck to deck. The system includes three component systems: a deploying floor to safety barrier system that folds down to become a traversable floor when vertical translation is not in use and deploys to become a safety barrier during vertical translations; a folding ladder that stows in the ceiling when not in use; and an elevator platform that can attach to the ladder for cargo or incapacitated crew member transport. This system is complemented by a Horizontal Translation System, also based in part on the same GrabCAD challenge, provides for horizontal crew translation in microgravity but requires no vehicle reconfiguration for fractional or whole gravity operation. It relies primarily on gecko-inspired grippers, originally developed at Stanford and NASA JPL, attached to footwear to enable crew to emulate walking. Some handrails are also strategically placed throughout the Common Habitat to aid translation in both gravity and microgravity environments.

Common Habitat↗

Oxygen Solubility in Aqueous Amine Solvents with Common Additives Used for CO 2 Chemical Absorption

Major sources of anthropogenic CO 2 are power generation and transportation industries where researcher continue to explore CO 2 emission mitigation strategies as applied to these CO 2 sources through carbon capture, utilization, and sequestration (CCUS). The most mature CO 2 capture technology is post-combustion carbon capture (PCCC) using aqueous amine solutions/solvents, however solvent degradation and regeneration costs are slowing the widespread adoption of PCCC. Solvent degradation of the aqueous amine solutions is mainly caused from the temperature gradient between the absorber and stripper columns and common flue gas components such as SOx, NOx, and oxygen (O 2 ). The two main classifications of solvent degradation are thermal degradation, occurring when the amine reacts with itself at elevated temperatures and anaerobic conditions and oxidative degradation. Oxidative degradation reactions can occur from oxygen mass transfer and free radical oxidation. Metals, such as iron from the corrosion of steel structures used in industrial applications such as PCCC, can help to catalyze these reactions. Various oxidative degradation studies have shown how O 2 concentrations in flue gas and, to a lesser extent, temperature influence the extent of oxidative degradation. Accurately measuring the O 2 solubility, commonly referred to as dissolved oxygen (DO), in a quick, continuous, and efficient manner in aqueous amine solvents should contribute to determine the effectiveness of mitigation strategies for oxidative degradation. Knowing that commercial PCCC amine solvents contain components beyond water, amine, carbonate species and CO 2 , this investigation was conducted to determine the oxygen solubility changes of common aqueous amines solutions with commonly used and published solvent additives. The impact of carbon loadings with and without the additives was also examined. A commercial dissolved oxygen probe was used to measure the DO concentrations and compared them against standard Winkler Method titration values. The results show that antifoam shows minimal change in [DO]. MBT yielded lower DO values, and NaVO 3 showed a higher DO concentration due to interferences. These results indicate that most common amine solvent additives should be expected to minimally impact oxygen solubility and amine oxidative degradation.

60 APPLIED LIFE SCIENCES↗

Space Station Freedom electrical power system hardware commonality with the United States Polar Platform

The National Aeronautics and Space Administration has adopted the policy to achieve the maximum practical level of commonality for the Space Station Freedom program in order to significantly reduce life cycle costs. Commonality means using identical or similar hardware/software for meeting common sets of functionally similar requirements. Information on how the concept of commonality is being implemented with respect to electric power system hardware for the Space Station Freedom and the U.S. Polar Platform is presented. Included is a historical account of the candidate common items which have the potential to serve the same power system functions on both Freedom and the Polar Platform.

Rieker, Lorra L.↗

The principle of commonality and its application to the Space Station Freedom Program

The principle of commonality has achieved wide application in the communication, automotive, and aircraft industries. By the use of commonality, component development costs are minimized, logistics are simplified, and the investment costs of spares inventory are reduced. With space systems, which must be maintained and repaired in orbit, the advantages of commonality are compounded. Transportation of spares is expensive, on-board storage volume for spares is limited, and crew training and special tools needed for maintenance and repair are significant considerations. This paper addresses the techniques being formulated to realize the benefits of commonality in the design of the systems and elements of the Space Station Freedom Program, and include the criteria for determining the extent of commonality to be implemented.

Hopson, George D.↗

Common Cause Case Study: An Estimated Probability of Four Solid Rocket Booster Hold-down Post Stud Hang-ups

Until Solid Rocket Motor ignition, the Space Shuttle is mated to the Mobil Launch Platform in part via eight (8) Solid Rocket Booster (SRB) hold-down bolts. The bolts are fractured using redundant pyrotechnics, and are designed to drop through a hold-down post on the Mobile Launch Platform before the Space Shuttle begins movement. The Space Shuttle program has experienced numerous failures where a bolt has "hung-up." That is, it did not clear the hold-down post before liftoff and was caught by the SRBs. This places an additional structural load on the vehicle that was not included in the original certification requirements. The Space Shuttle is currently being certified to withstand the loads induced by up to three (3) of eight (8) SRB hold-down post studs experiencing a "hang-up." The results af loads analyses performed for four (4) stud-hang ups indicate that the internal vehicle loads exceed current structural certification limits at several locations. To determine the risk to the vehicle from four (4) stud hang-ups, the likelihood of the scenario occurring must first be evaluated. Prior to the analysis discussed in this paper, the likelihood of occurrence had been estimated assuming that the stud hang-ups were completely independent events. That is, it was assumed that no common causes or factors existed between the individual stud hang-up events. A review of the data associated with the hang-up events, showed that a common factor (timing skew) was present. This paper summarizes a revised likelihood evaluation performed for the four (4) stud hang-ups case considering that there are common factors associated with the stud hang-ups. The results show that explicitly (i.e. not using standard common cause methodologies such as beta factor or Multiple Greek Letter modeling) taking into account the common factor of timing skew results in an increase in the estimated likelihood of four (4) stud hang-ups of an order of magnitude over the independent failure case.

Cross, Robert↗

Utilizing a Common Model Architecture for the Simulation of On-Orbit Human Spaceflight Operations

Advances in computing and simulation technology during the 1980 s promoted the development of multiple simulations for on-orbit human spaceflight operations at the National Aeronautics and Space Administration (NASA) Johnson Space Center (JSC). By the late 1980 s, it became increasingly clear that redundancy risked leading to inefficiencies in dissemination and incorporation of models across simulations, institutional organizations, and spaceflight programs. Software development tools, such as the Trick Simulation Environment, began to emerge providing a generalized framework for the development, integration, and operation of simulations. Throughout the 1990s, engineering, operations, and training simulations began to migrate to Trick in order to take advantage of a common simulation environment. Due to forecasted reductions in funding to the Shuttle and International Space Station (ISS) programs in the early 2000s, the common framework that arose from this simulation migration was formalized as the Common Model Architecture (CMA). The CMA was established in order to further reduce redundancy, while promoting model sharing and enhancing model integrity. A minimal set of standards for data flows, functional interactions, and model organization based on Trick were defined for those features found to be most important in facilitating model interaction. Model-providing organizations within NASA JSC took ownership of models for their specific areas of expertise, and agreements were reached on common model sharing and usage. This paper describes the evolution of commonality enabled by the Trick Simulation Environment at JSC leading to the CMA, the standards defined by the CMA, a recent CMA simulation example, and potential application of the CMA to NASA s new Exploration program.

Leslie J Quiocho↗

Common Cause Case Study: An Estimated Probability of Four Solid Rocket Booster Hold-Down Post Stud Hang-ups

Until Solid Rocket Motor ignition, the Space Shuttle is mated to the Mobil Launch Platform in part via eight (8) Solid Rocket Booster (SRB) hold-down bolts. The bolts are fractured using redundant pyrotechnics, and are designed to drop through a hold-down post on the Mobile Launch Platform before the Space Shuttle begins movement. The Space Shuttle program has experienced numerous failures where a bolt has hung up. That is, it did not clear the hold-down post before liftoff and was caught by the SRBs. This places an additional structural load on the vehicle that was not included in the original certification requirements. The Space Shuttle is currently being certified to withstand the loads induced by up to three (3) of eight (8) SRB hold-down experiencing a "hang-up". The results of loads analyses performed for (4) stud hang-ups indicate that the internal vehicle loads exceed current structural certification limits at several locations. To determine the risk to the vehicle from four (4) stud hang-ups, the likelihood of the scenario occurring must first be evaluated. Prior to the analysis discussed in this paper, the likelihood of occurrence had been estimated assuming that the stud hang-ups were completely independent events. That is, it was assumed that no common causes or factors existed between the individual stud hang-up events. A review of the data associated with the hang-up events, showed that a common factor (timing skew) was present. This paper summarizes a revised likelihood evaluation performed for the four (4) stud hang-ups case considering that there are common factors associated with the stud hang-ups. The results show that explicitly (i.e. not using standard common cause methodologies such as beta factor or Multiple Greek Letter modeling) taking into account the common factor of timing skew results in an increase in the estimated likelihood of four (4) stud hang-ups of an order of magnitude over the independent failure case.

Cross, Robert↗

Characterization of a Common-Gate Amplifier Using Ferroelectric Transistors

In this paper, the empirical data collected through experiments performed using a FeFET in the common-gate amplifier circuit is presented. The FeFET common-gate amplifier was characterized by varying all parameters in the circuit, such as load resistance, biasing of the transistor, and input voltages. Due to the polarization of the ferroelectric layer, the particular behavior of the FeFET common-gate amplifier presents interesting results. Furthermore, the differences between a FeFET common-gate amplifier and a MOSFET common-gate amplifier are examined.

Hunt, Mitchell↗

Commonality of Ground Systems in Launch Operations

NASA is examining the utility of requiring a certain degree of commonality in both flight and ground systems in the Constellation Program. While the benefits of commonality seem obvious in terms of minimizing upfront development and long-term operations and maintenance costs, success in real, large-scale engineering systems used to support launch operations is relatively unknown. A broad literature review conducted for this paper did not yield a single paper specifically addressing the application of commonality for ground systems at any launch site in the United States or abroad. This paper provides a broad overview of the ground systems, captures historical and current application of commonality at the launch site, and offers suggestions for additional research to further develop commonality approaches.

Quinn, Shawn M.↗

The Deep Space Network in the Common Platform Era: A Prototype Implementation at DSS-13

To enhance NASA's Deep Space Network (DSN), an effort is underway to improve network performance and simplify its operation and maintenance. This endeavor, known as the "Common Platform," has both short- and long-term objectives. The long-term work has not begun yet; however, the activity to realize the short-term goals has started. There are three goals for the long-term objective: 1. Convert the DSN into a digital network where signals are digitized at the output of the down converters at the antennas and are distributed via a digital IF switch to the processing platforms. 2. Employ a set of common hardware for signal processing applications, e.g., telemetry, tracking, radio science and Very Long Baseline Interferometry (VLBI). 3. Minimize in-house developments in favor of purchasing commercial off-the-shelf (COTS) equipment. The short-term goal is to develop a prototype of the above at NASA's experimental station known as DSS-13. This station consists of a 34m beam waveguide antenna with cryogenically cooled amplifiers capable of handling deep space research frequencies at S-, X-, and Ka-bands. Without the effort at DSS-13, the implementation of the long-term goal can potentially be risky because embarking on the modification of an operational network without prior preparations can, among other things, result in unwanted service interruptions. Not only are there technical challenges to address, full network implementation of the Common Platform concept includes significant cost uncertainties. Therefore, a limited implementation at DSS-13 will contribute to risk reduction. The benefits of employing common platforms for the DSN are lower cost and improved operations resulting from ease of maintenance and reduced number of spare parts. Increased flexibility for the user is another potential benefit. This paper will present the plans for DSS-13 implementation. It will discuss key issues such as the Common Platform architecture, choice of COTS equipment, and the standard for radio frequency (RF) to digital interface.

Space Communications and Navigation (SCaN)↗

Analysis of Multi-Flight Common Routes for Traffic Flow Management

This paper presents an approach for creating common weather avoidance reroutes for multiple flights and the associated benefits analysis, which is an extension of the single flight advisories generated using the Dynamic Weather Routes (DWR) concept. These multiple flight advisories are implemented in the National Airspace System (NAS) Constraint Evaluation and Notification Tool (NASCENT), a nation-wide simulation environment to generate time- and fuel-saving alternate routes for flights during severe weather events. These single flight advisories are clustered together in the same Center by considering parameters such as a common return capture fix. The clustering helps propose routes called, Multi-Flight Common Routes (MFCR), that avoid weather and other airspace constraints, and save time and fuel. It is expected that these routes would also provide lower workload for traffic managers and controllers since a common route is found for several flights, and presumably the route clearances would be easier and faster. This study was based on 30-days in 2014 and 2015 each, which had most delays attributed to convective weather. The results indicate that many opportunities exist where individual flight routes can be clustered to fly along a common route to save a significant amount of time and fuel, and potentially reducing the amount of coordination needed.

weather uncertainty↗

A Common Probe Design for Multiple Planetary Destinations

Atmospheric probes have been successfully flown to planets and moons in the solar system to conduct in-situ measurements. They include the Pioneer Venus multi-probes, the Galileo Jupiter probe, and Huygens probe. Probe mission concepts to five destinations, including Venus, Jupiter, Saturn, Uranus, and Neptune, have all utilized similar-shaped aeroshells and concept of operations, namely a 45 deg sphere cone shape with high density heatshield material and parachute system for extracting the descent vehicle from the aeroshell. Each concept designed its probe to meet specific mission requirements and to optimize mass, volume, and cost. At the 2017 IPPW, NASA Headquarters postulated that a common aero-shell design could be used successfully for multiple destinations and missions. This "common probe" design could even be assembled with multiple copies, properly stored, and made available for future NASA missions, potentially realizing savings in cost and schedule and reducing the risk of losing technologies and skills difficult to sustain over decades. Thus the NASA Planetary Science Division funded a study to investigate whether a common probe design could meet most, if not all, mission needs to the five planetary destinations with extreme entry environments. The Common Probe study involved four NASA Centers and addressed these issues, including constraints and inefficiencies that occur in specifying a common design.Study methodology: First, a notional payload of instruments for each destination was defined based on priority measurements from the Planetary Science Decadal Survey. Steep and shallow entry flight path angles (EFPA) were defined for each planet based on qualification and operational g-load limits for current, state-of-the-art instruments. Interplanetary trajectories were then identified for a bounding range of EFPA. Next, 3-DoF simulations for entry trajectories were run using the entry state vectors from the interplanetary trajectories. Aeroheating correlations were used to generate stagnation point convective and radiative heat flux profiles for several aeroshell shapes and entry masses. High fidelity thermal response models for various TPS materials were used to size stagnation point thicknesses, with margins based on previous studies. Backshell TPS masses were assumed based on scaled heat fluxes from the heatshield and also from previous mission concepts.

Hwang, Helen H.↗

Internal Architecture of the Common Habitat

The core stage liquid oxygen tank of the Space Launch System can be manufactured as a habitat instead of as a propellant tank, with a common design such that it is equally suitable for use in 0g, 1/6g, 3/8g, 1g, or variable artificial gravity. It is capable of sustaining a crew size of eight for missions up to 1200 days in duration. This Common Habitat can be the central element of a human spaceflight architecture that encompasses the Moon, Mars, and other destinations within the inner solar system. Within this archtiecture, the Common Habitat is specifically used as the core habitation element within a Lunar Base Camp, Mars Base Camp, and the Deep Space Exploration Vehicle. The Common Habitat internal architecture applies a design philosophy to separate crew functions according to deck. The lower deck is reserved for private functions. It includes eight private crew quarters and four waste and hygiene clusters – each with a private waste management compartment, private full body hygiene compartment, and private foyer/clothes changing area. The mid deck is primarily allocated to mission-related functions. It includes an exercise facility, fabrication / maintenance / repair facility, physical science laboratory (physics, geology, and remote sensing: astronomy, heliophysics, planetary science, and Earth science), and life science laboratory (biology and human research). The mid deck also has four external hatches, clocked one every 90 degrees, centered on the vehicle vertical centerline. Each hatch has a 60-inch tall by 40-inch wide opening with the mid deck floor 16 inches below the bottom lip of the hatch opening. The upper deck also includes some mission functions, but is primarily allocated to social functions. It includes a large galley, wardroom with projector and display screen, plant growth chambers, bulk stowage, command and control station, medical facility, hygiene compartment, and vehicle subsystems.

Habitability↗

Common Habitat Architecture

The Common Habitat is a human habitation element for inner solar system long duration (assessed up to 1200 days) space missions, inclusive of both orbital and surface missions. Exploration Science is supported by both internal laboratories and external science platforms (e.g., telescopes and other remote sensing systems). The Common Habitat is integrated with other elements needed (e.g., propulsion, heat rejection, airlock, etc.) to make the total system a spacecraft, space station, base, or other facility. The Common Habitat Architecture is an exploratory, primarily volunteer, NASA conceptual study and is NOT the current Artemis architecture. Common Habitat elements do show a similarity to some elements under development for Artemis but do not imply any relationship to, influence on, or influence by Artemis requirements, objectives, or acquisitions.

Common Habitat↗

A Crew and Logistics Lander for the Common Habitat Architecture

The Common Habitat Architecture is a conceptual study that explores the use of a large habitat derived from the Space Launch System (SLS) liquid oxygen tank as a core habitation element that can be used for crew missions or training in 0g, 1/6g, 3/8g, and 1g. This is not part of current NASA Artemis mission planning, but instead represents an architecture that could potentially follow after the Artemis missions and initial human Mars landings. This architecture leverages Starship-derived vehicles for crew landing on and ascent from the Moon and Mars in support of long-duration surface missions with pressurized crew transfer baselined as a nominal capability. This is not the Human Landing System variant of Starship but represents a number of modifications to enable long-duration surface missions with an eight-person crew size. A rapid brainstorming study was conducted in February of 2021, using only public data, to identify options to deliver the 90-ton Common Habitat to the surface and emplace it at the intended habitation site. This study compared three lunar lander concepts and three Mars lander concepts before ultimately selecting the SpaceX Starship as the most viable lander. The Common Habitat Architecture assumes that this Starship variant can further be modified for crew and cargo delivery, assuming that the use of a common system will lead to cost benefits. It is assumed that the Starship will expend too much propellant in landing crew and logistics to be able to launch, given the assumed absence of surface propellant production. Consequently, a separable ascent stage is used for crew ascent. The pressurized elements of this modified Starship are discussed: Starship Ascent Module, Airlock, Transfer Tunnel, Pressurized Crew Transfer Module, and Logistics Modules. For each element, a description, dimensions, rough mass estimates, core capabilities, and design features are presented. Key mechanisms and internal structures of the starship are also discussed. This will include flame diverters for the ascend module, the orbital docking hatch, Pressurized Crew Transfer Module Garage door, Starship Ascent Module fairing, flame diverter blow-out panels, Pressurized Crew Transfer Module lift system, logistics module lift system, contingency crew ascent via the logistics module lift system, internal catwalks, and structural interfaces. Concept of Operations will be discussed for both Moon and Mars. This will include in-space crew rendezvous and transfer, crewed landing, shirtsleeve crew transfer, crew departure, contingency surface operations, and final element disposition. Key differences for Mars will be discussed, such as the Martian atmosphere, dust storms, the absence of crew handover, and Deep Space Exploration Vehicle rendezvous. Contingency microgravity maintenance access will also be discussed. This work will demonstrate viable pressurized crew transfer with a Starship-based lander architecture. Forward work includes Garage lighting and camera systems, Starship Ascent Module propulsion system and docking port trades, contingency habitation trades, and mass/power equipment estimation. Finally, forward work includes developing a heavy cargo return system derived from the Crew and Logistics Starship. The goal of this system is the return of at least 10,000 kg payloads from the surfaces of the Moon and Mars.

Lunar Lander↗

Onyx Embolization for Occlusion of the Proximal Internal Iliac Artery During EVAR in Patients with Unsuitable Landing Zones in the Common Iliac Artery

Background: Twenty percent of the patients with AAA have an aneurysm involving the common iliac arteries. Large common iliac diameter can be treated with an iliac branched device or extension of the stent graft to the external iliac artery with occlusion of the ipsilateral internal iliac artery (IIA) to prevent type 2 endoleaks. This study describes and evaluates a embolization technique using Onyx in conjunction with EVAR in aneurysms with poor landing zones in the common iliac arteries. Methods: Patients with Onyx IIA embolization during EVAR, identified from the hospital operating code database, constitute the study population. Onyx embolization was performed by injection at the IIA origin. Peri- and postoperative complications were collected from the medical records. Thin-sliced CT scan was performed 1 month and 1 year after the procedure. Results: Thirty-six patients with complex iliac anatomy and insufficient landing zones (without sealing possibility for standard stent grafts) were identified out of 243 consecutive EVAR treatments during a 13-year period. In seventeen patients (7%), the IIA was embolized with Onyx. Technical success was obtained in all 17 patients, without adverse event or procedural complication. No complication related to the embolization procedure was noted during follow-up. Conclusions: During EVAR treatment of patients with aneurysm involving the common iliac artery, Onyx embolization of IIA is a feasible option without need of selective catheterization of the IIA orifice, potentially preserving important branches of the IIA and simplifying emergency procedures.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Microbial functional genes commonly respond to elevated carbon dioxide

Atmospheric CO 2 concentration is increasing, largely due to anthropogenic activities. Previous studies of individual free-air CO 2 enrichment (FACE) experimental sites have shown significant impacts of elevated CO 2 (eCO 2 ) on soil microbial communities; however, no common microbial response patterns have yet emerged, challenging our ability to predict ecosystem functioning and sustainability in the future eCO 2 environment. Here we analyzed 66 soil microbial communities from five FACE sites, and showed common microbial response patterns to eCO 2 , especially for key functional genes involved in carbon and nitrogen fixation (e.g., pcc/acc for carbon fixation, nifH for nitrogen fixation), carbon decomposition (e.g., amyA and pulA for labile carbon decomposition, mnp and lcc for recalcitrant carbon decomposition), and greenhouse gas emissions (e.g., mcrA for methane production, norB for nitrous oxide production) across five FACE sites. Also, the relative abundance of those key genes was generally increased and directionally associated with increased biomass, soil carbon decomposition, and soil moisture. In addition, a further literature survey of more disparate FACE experimental sites indicated increased biomass, soil carbon decay, nitrogen fixation, methane and nitrous oxide emissions, plant and soil carbon and nitrogen under eCO 2 . A conceptual framework was developed to link commonly responsive functional genes with ecosystem processes, such as pcc/acc vs. soil carbon storage, amyA/pulA/mnp/lcc vs. soil carbon decomposition, and nifH vs. nitrogen availability, suggesting that such common responses of microbial functional genes may have the potential to predict ecosystem functioning and sustainability in the future eCO 2 environment.

59 BASIC BIOLOGICAL SCIENCES↗