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DOE OSTI2025

Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography

Cryo-electron tomography (cryo-ET) is a powerful modality for resolving cellular structures in their native state. While single-particle cryo-electron microscopy excels in determining protein structures purified from recombinant or endogenous sources due to an abundance of particles, weak contrast issues are accentuated in cryo-ET by low copy numbers in crowded cellular milieux. Continuous laser phase plates offer improved contrast in cryo-ET; however, their implementation demands exceptionally high-peak optical intensities. Instead, a novel experimental approach to enhance contrast in cryo-ET is to manipulate the phase of scattered pulsed electrons using ultrafast pulsed photons. Here, we outline the experimental design of a proof-of-concept electron microscope and demonstrate synchronization between electron packets and laser pulses. Furthermore, we show ultrabright photoemission of electrons from an alloy field emission tip using femtosecond ultraviolet pulses. These experiments pave the way toward exploring the utility of the ponderomotive effect using pulsed radiation to increase phase contrast in cryo-ET of subcellular protein complexes in situ, thus advancing the field of cell biology.

Du, Daniel X. [Columbia Univ., New York, NY (Unite↗

DOE OSTI2019

The application of cinematic rendering to CT evaluation of upper tract urothelial tumors: principles and practice

Upper tract urothelial carcinoma (UTUC) is a relatively uncommon but aggressive genitourinary malignancy for which multi-phase contrast-enhanced computed tomography (CT) plays an important role in evaluation and staging. 3D imaging with maximum intensity projection (MIP) and volume-rendered (VR) images has been described as a useful means of evaluating UTUC. In this study, we describe the technique of a novel 3D methodology known as cinematic rendering (CR) and provide clinical examples of UTUC visualized with CR. CR utilizes a complex universal lighting model in order to create photorealistic images with improved detail and depth in comparison to MIP or VR images. In the case of UTUC, CR can be used in different contrast phases to show abnormally thickened and enhancing urothelium or filling defects in the renal collecting system or ureters in the excretory phase. CR images can also be manipulated in order to generate translucent views of the upper urinary tract in order to add conspicuity to intraluminal findings.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

NASA NTRS1983

Application of image processing technology to problems in manuscript encapsulation

The long term effects of encapsulation individual sheets of the Codex Hammer were investigated. The manuscript was simulated with similar sheets of paper which were photographed under repeatable raking light conditions to enhance their surface texture, encapsulated in plexiglas, cycled in an environmental test chamber, and rephotographed at selected intervals. The film images were digitized, contrast enhanced, geometrically registered, and apodized. An FFT analysis of a control sheet and two experimental sheets indicates no micro-burnishing, but reveals that the ""mesoscale'' deformations with sizes 8mm are degrading monotonically, which is of no concern. Difference image analysis indicates that the sheets were increasingly stressed with time and that the plexiglas did not provide a sufficient environmental barrier under the simulation conditions. The relationship of these results to the Codex itself is to be determined.

Glackin, D. L.↗

NASA NTRS2004

DSP Implementation of the Retinex Image Enhancement Algorithm

The Retinex is a general-purpose image enhancement algorithm that is used to produce good visual representations of scenes. It performs a non-linear spatial/spectral transform that synthesizes strong local contrast enhancement and color constancy. A real-time, video frame rate implementation of the Retinex is required to meet the needs of various potential users. Retinex processing contains a relatively large number of complex computations, thus to achieve real-time performance using current technologies requires specialized hardware and software. In this paper we discuss the design and development of a digital signal processor (DSP) implementation of the Retinex. The target processor is a Texas Instruments TMS320C6711 floating point DSP. NTSC video is captured using a dedicated frame-grabber card, Retinex processed, and displayed on a standard monitor. We discuss the optimizations used to achieve real-time performance of the Retinex and also describe our future plans on using alternative architectures.

Hines, Glenn↗

NASA NTRS1974

The application of image enhancement techniques to remote manipulator operation

Methods of image enhancement which can be used by an operator who is not experienced with the mechanisms of enhancement to obtain satisfactory results were designed and implemented. Investigation of transformations which operate directly on the image domain resulted in a new technique of contrast enhancement. Transformations on the Fourier transform of the original image, including such techniques as homomorphic filtering, were also investigated. The methods of communication between the enhancement system and the computer operator were analyzed, and a language was developed for use in image enhancement. A working enhancement system was then created, and is included.

Gonzalez, R. C.↗

NASA NTRS2004

DSP Implementation of the Multiscale Retinex Image Enhancement Algorithm

The Retinex is a general-purpose image enhancement algorithm that is used to produce good visual representations of scenes. It performs a non-linear spatial/ spectral transform that synthesizes strong local contrast enhancement and color constancy. A real-time, video frame rate implementation of the Retinex is required to meet the needs of various potential users. Retinex processing contains a relatively large number of complex computations, thus to achieve real-time performance using current technologies requires specialized hardware and software. In this paper we discuss the design and development of a digital signal processor (DSP) implementation of the Retinex. The target processor is a Texas Instruments TMS320C6711 floating point DSP. NTSC video is captured using a dedicated frame grabber card, Retinex processed, and displayed on a standard monitor. We discuss the optimizations used to achieve real-time performance of the Retinex and also describe our future plans on using alternative architectures.

Hines, Glenn D.↗

DOE OSTI2023

Increased range and contrast in fog with circularly polarized imaging

Fogs, low lying clouds, and other highly scattering environments pose a challenge for many commercial and national security sensing systems. Current autonomous systems rely on optical sensors for navigation whose performance is degraded by highly scattering environments. In our previous simulation work, we have shown that polarized light can penetrate through a scattering environment such as fog. We have demonstrated that circularly polarized light maintains its initial polarization state better than linearly polarized light, even through large numbers of scattering events and thus ranges. This has recently been experimentally verified by other researchers. In this work, we present the design, construction, and testing of active polarization imagers at short-wave infrared and visible wavelengths. We explore multiple polarimetric configurations for the imagers, focusing on linear and circular polarization states. The polarized imagers were tested at the Sandia National Laboratories Fog Chamber under realistic fog conditions. Here, we show that active circular polarization imagers can increase range and contrast in fog better than linear polarization imagers. We show that when imaging typical road sign and safety retro-reflective films, circularly polarized imaging has enhanced contrast throughout most fog densities/ranges compared to linearly polarized imaging and can penetrate over 15 to 25 m into the fog beyond the range limit of linearly polarized imaging, with a strong dependence on the interaction of the polarization state with the target materials.

47 OTHER INSTRUMENTATION↗

NASA NTRS1978

Color enhancement of landsat agricultural imagery: JPL LACIE image processing support task

Color enhancement techniques were applied to LACIE LANDSAT segments to determine if such enhancement can assist analysis in crop identification. The procedure involved increasing the color range by removing correlation between components. First, a principal component transformation was performed, followed by contrast enhancement to equalize component variances, followed by an inverse transformation to restore familiar color relationships. Filtering was applied to lower order components to reduce color speckle in the enhanced products. Use of single acquisition and multiple acquisition statistics to control the enhancement were compared, and the effects of normalization investigated. Evaluation is left to LACIE personnel.

Madura, D. P.↗

NASA NTRS2019

A Qualitative Investigation of Selected Infrared Flow Visualization Image Processing Techniques

The deployment and integration of high-sensitivity infrared cameras in a transonic wind tunnel testenvironment has resulted in a unique capability to image aerodynamic phenomena in real-time. Multi-camera infrared flow visualization data systems are now routinely utilized at the NASA Ames Unitary Plan Wind Tunnel. The small flow-induced temperature gradients on the surface of the wind tunnel test article coupled with the high bit-depth of the infrared camera sensor makes the processing of the image data critically important. An image processing routine must enhance features of interest with minimal artifacts. Additionally, the production wind tunnel test environment demands that these processed images are made available in a real-time, automatic fashion. Therefore, any image processing routine must be computationally economical and enhance the image data with minimal input from a human operator. The following seeks to qualitatively explore selected image processing techniques by assessing their effectiveness to resolve flow features on a wind tunnel test article. A multi-scale contrast enhancement technique is discussed as well as a new implementation of a multi-scale, non-interpolated adaptive histogram equalization. Finally, a novel method is introduced that demonstrates the ability to resolve flow features imaged on bare-steel test articles possessing low emissivity.This method makes use of dynamic mode decomposition and discrete-time filtering to separate the background reflections that dominate low emissivity surfaces from the aerodynamic driven surface temperature gradients.This process will be shown to resolve the onset of boundary layer transition on a bare metal wing as well as identify and resolve hidden features in the image data. While the implementation of this technique is very preliminary it demonstrates the potential to extend the application of infrared flow-visualization within the wind tunnel test environment.

Image Processeing↗

DOE OSTI2022

Synchrotron X-ray based particle image velocimetry to measure multiphase streamflow and densitometry

Synchrotron-based radiation techniques are increasingly used in the field of flow condition measurements. As a complicated multiphase flow, cavitation has been investigated in a wide variety of industrial fields, to prevent damages on spillways at large dams or intensify the bacteria eradication rate during water treatments. To control the detrimental effects of cavitating flows, the dynamics of and mechanisms affecting cavitation development shall be visualized and identified in real-time. Due to the large reflection and scattering effects of multiphase flow, standard visible-light imaging technologies cannot penetrate vapor bubbles and provide extremely limited information. In this study, the synchrotron source emitted high-flux X-ray pulses are used to capture flow motion and visualize internal structures with sufficiently high spatial and temporal resolutions. The image processing procedures on raw x-ray images are developed using 2D Fourier transform (FT) and wavelet transform (WT) to implement the contrast enhancement and de-noising. Through the edge detection algorithm, tracking either seeded particles or phase interfaces inside the opaque multiphase flow can be achieved to perform the particle image velocimetry. Further, with decomposing particle-only and particle-eliminated subplots from the X-ray phase-contrast image, the time-resolved velocity and void fraction fields are obtained simultaneously, paving a way for further flow condition analysis such as densitometry.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

DOE OSTI2019

What a difference a delay makes! CT urogram: a pictorial essay

Purpose: The aim of this pictorial essay is to demonstrate several cases where the diagnosis would have been difficult or impossible without the excretory phase image of CT urography. Methods: A brief discussion of CT urography technique and dose reduction is followed by several cases illustrating the utility of CT urography. Results: CT urography has become the primary imaging modality for evaluation of hematuria, as well as in the staging and surveillance of urinary tract malignancies. CT urography includes a non-contrast phase and contrast-enhanced nephrographic and excretory (delayed) phases. While the three phases add to the diagnostic ability of CT urography, it also adds potential patient radiation dose. Several techniques including automatic exposure control, iterative reconstruction algorithms, higher noise tolerance, and split-bolus have been successfully used to mitigate dose. The excretory phase is timed such that the excreted contrast opacifies the urinary collecting system and allows for greater detection of filling defects or other abnormalities. Sixteen cases illustrating the utility of excretory phase imaging are reviewed. Conclusions: Excretory phase imaging of CT urography can be an essential tool for detecting and appropriately characterizing urinary tract malignancies, renal papillary and medullary abnormalities, CT radiolucent stones, congenital abnormalities, certain chronic inflammatory conditions, and perinephric collections.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

NASA NTRS1991

Polarization Filtering of SAR Data

Theoretical analysis of polarization filtering of synthetic-aperture-radar (SAR) returns provide hybrid method applied to either (1) maximize signal-to-noise ratio of return from given target or (2) enhance contrast between targets of two different types (that have different polarization properties). Method valid for both point and extended targets and for both monostatic and bistatic radars as well as SAR. Polarization information in return signals provides more complete description of radar-scattering properties of targets and used to obtain additional information about targets for use in classifying them, discriminating between them, or enhancing features of radar images.

Dubois, Pascale C.↗

DOE OSTI2022

Sequences of seismic and aseismic slip on bimaterial faults show dominant rupture asymmetry and potential for elevated seismic hazard

Here we perform numerical simulations of sequences of earthquake and aseismic slip on planar rate and state faults separating dissimilar material within the 2-D plane strain approximation. We resolve all stages of the earthquake cycle from aseismic slip to fast ruptures while incorporating full inertia effects during seismic event propagation. We show that bimaterial coupling results in favorable nucleation site and subsequent asymmetric rupture propagation. We demonstrate that increasing the material contrast enhances this asymmetry leading to higher slip rates and normal stress drops in the preferred rupture propagation direction. The normal stress drop, induced by the bimaterial effect, leads to strong dynamic weakening of the fault and may destabilize the creeping region on a heterogeneous rate and state fault, resulting in extended rupture propagation. Such rupture penetration into creeping patches may lead to more frequent opening of earthquake gates, causing increased seismic hazard. Furthermore, bimaterial coupling may lead to irregular seismicity pattern in terms of event length, peak slip rates, and hypocenter location, depending on the properties of the creeping patches bordering the seismogenically active part of the fault. Our results highlight robust characteristics of bimaterial interfaces that persist over long sequence of events and suggest the need for further exploration of the role of material contrast in earthquake physics and models of seismic hazard.

58 GEOSCIENCES↗

DOE OSTI2021

Simultaneous electrocatalytic hydrogenation of aldehydes and phenol over carbon-supported metals

Electrocatalytic hydrogenation of furfural, benzaldehyde, and phenol was studied on carbon-supported Rh, Ru, Pd, and Cu catalysts. All metals were active for hydrogenation of the carbonyl group in furfural and benzaldehyde, but only Rh/C was active for phenol hydrogenation. The intrinsic activities for furfural and benzaldehyde conversion were Pd/C < Ru/C ? Rh/C < Cu/C and Ru/C < Rh/C < Pd/C < Cu/C, respectively. While the trend in furfural hydrogenation remained the same in the presence of phenol, the trend in benzaldehyde hydrogenation in the presence of phenol changed to Rh/C < Ru/C < Pd/C < Cu/C. The Faradaic efficiencies for hydrogenation of both furfural (in the 10–40% range) and benzaldehyde (in the 45–100% range) followed the trend: Cu/C < Rh/C < Ru/C < Pd/C. When phenol and an aldehyde were present in the reactant solution, phenol hydrogenation (only observed on Rh) was suppressed. In contrast, enhancements in the rates of carbonyl reduction were observed for both aldehydes in presence of phenol. Such enhancements depend on both the aldehyde and the metal catalyst. The enhancing factors for hydrogenation of furfural and benzaldehyde were in the 1.5–2 and 2–4 ranges, respectively. We conclude that intermolecular interactions between adsorbed phenol and the aldehydes is a general phenomenon that can potentially enhance the rates of carbonyl hydrogenation. This work highlights co-adsorption effects that pose both challenges and advantages for electrocatalytic reduction of organic compounds.

Electrocatalytic hydrogenation, metal catalysts, p↗

DOE OSTI2022

A New Workflow of X-ray CT Image Processing and Data Analysis of Structural Features in Rock Using Open-Source Software

X-ray computed tomography (CT) images of rock specimens often contain artifacts which must be corrected before scientific analyses are performed. Here, we present a new workflow of automated image processing to utilize poor-quality X-ray CT scan images. The workflow runs on the open-source image analysis software and efficiently separates desired features from low-contrast scanned images. The new workflow is a two-step technique using contrast enhancement and automated feature segmentation to generate noise-free binary images. The results of binary images using the proposed workflow and using a conventional thresholding technique are analyzed to show the quality of the proposed method. The paper also presents a workflow of estimating the structural geometries of features in two and three dimensions. The results of the structural feature analyses and computational time were compared between the open-source (ImageJ) and commercial image analysis software (Bruker Computed Tomography Analyzer). The commercial software was more computationally efficient, but the task-specific macros in open-source software enabled the user-desired automation in image processing and data extraction of desired structural features of comparable quality.

47 OTHER INSTRUMENTATION↗

DOE OSTI2026

NanoPSD: A software for automatic detection of Nano-Particle Shape Distribution in electron microscopy images

Accurate quantification of the size and morphology of nanoparticles from electron microscopy (EM) images is essential to understand growth mechanisms, surface reactivity, and functional behavior in nanoscale materials. Manual analysis remains slow, subjective, and difficult to reproduce in large datasets. We introduce NanoPSD (Nano-Particle Shape Distribution), an open-source and fully automated framework for quantitative particle detection and morphology analysis from EM images. NanoPSD integrates adaptive contrast enhancement, polarity-agnostic scale-bar detection, Optical Character Recognition (OCR)-based calibration, and classical segmentation via Otsu thresholding with morphological refinement. Particle contours are used to extract geometric descriptors, including equivalent circular diameter, aspect ratio, circularity, and solidity, enabling automated classification into spherical, rod-like, and aggregate morphologies. The framework supports both single-image and batch processing, generating publication-quality visualizations, LaTeX-ready tables, and structured comma-separated values (CSV) datasets. As a demonstration, we applied NanoPSD to plasma-synthesized nanoparticle samples diagnosed via transmission electron microscopy (TEM). The code produced statistically robust size and morphology distributions spanning a few to tens of nanometers with minimal user supervision. The pipeline demonstrates high reproducibility and scalability, processing large image collections with consistent calibration and output formatting. Its modular design enables seamless integration of future deep-learning-based segmentation models, providing a pathway toward intelligent, data-driven electron microscopy analysis.

36 MATERIALS SCIENCE↗

DOE OSTI2021

Early MRI Blood Volume Changes in Constrictor Muscles Correlate With Postradiation Dysphagia

Predicting individual patient sensitivity to radiation therapy (RT) for tumor control or normal tissue toxicity is necessary to individualize treatment planning. In head and neck cancer, radiation doses are limited by many nearby critical structures, including structures involved in swallowing. Previous efforts showed that imaging parameters correlate with RT dose; here, we investigate the role of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) blood volume (BV) changes in predicting dysphagia.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

DOE OSTI2024

Shielding development for the spallation neutron source VENUS instrument

VENUS is a neutron imaging instrument that will use a broad range of neutron wavelengths, from epithermal to cold energies, and will include enhanced contrast mechanisms. It will offer novel energy-selective imaging techniques directly connecting complex engineering materials and systems’ structures, properties, and functions to reveal practical and fundamental answers about their real-world performance. Here, the instrument will be built at SNS beamline 10, facing the decoupled poisoned hydrogen moderator. The driving cost for the instrument is the beamline and instrument cave shielding. Final analyses were performed to evaluate the thickness and composition of shielding materials for the instrument cave and beamline to meet radiation safety criteria for the instrument to start up in 2024 after completing the SNS proton power upgrade.

43 PARTICLE ACCELERATORS↗