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Investigating the Cell Entry Mechanism, Disassembly, and Toxicity of the Nanocage PCC-1: Insights into Its Potential as a Drug Delivery Vehicle
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Synthesis and disassembly of an organometallic polymer comprising redox-active Co 4 S 4 clusters and Janus biscarbene linkers
Here, we show for the first time that main-chain organometallic polymers (MCOPs) can be prepared from Janus N-heterocyclic carbene (NHC) linkers and polynuclear cluster nodes. The crosslinked framework Co 4 S 4 -MCOP is synthesized via ligand displacement reactions and undergoes reversible electron transfer in the solid state. Discrete molecular cluster species can be excised from the framework by digesting the solid in solutions of excess monocarbene. Lastly, we demonstrate a synthetic route to monodisperse framework particles via coordination modulation.
Direct matter disassembly via electron beam control: electron-beam-mediated catalytic etching of graphene by nanoparticles
Here, we report electron-beam activated motion of a catalytic nanoparticle along a graphene step edge and associated etching of the edge. The catalytic hydrogenation process was observed to be activated by a combination of elevated temperature and electron beam irradiation. Reduction of beam fluence on the particle was sufficient to stop the process, leading to the ability to switch on and off the etching. Such an approach enables the targeting of individual nanoparticles to induce motion and beam-controlled etching of matter through activated electrocatalytic processes. The applications of electron-beam control as a paradigm for molecular-scale robotics are discussed.
Pit Disassembly and Processing/Oxidation Capacity (PDP) for Disposition of Surplus Plutonium at Los Alamos (FY2021 Workshop 1) [Slides]
This workshop will establish NNSA/LANL consensus on the planning basis for: updating the 2017 Life Cycle Cost Estimate (LCCE) pre-conceptual estimate by identifying the specific scope elements that will be included; identifying the specific scope elements that will be included in the Line Item project; and identifying the preliminary agenda items for the next PDP workshop.
Accidental Disassembly of a Multi Branch Outlet Strip with Surge Protector (Investigation Report)
On December 16 a worker attempted to disconnect a computer plug from a multi outlet surge protector strip. The plastic housing of the surge protector disintegrated while the plug was pulled exposing the bare conductors of the internal wiring. The worker disconnected the wall plug on the multi outlet strip and de-energized the outlet strip. The worker contacted the FOD and the FOD called the ISR DESO for advice. The DESO collected the pieces and performed an autopsy on the device.
AGR-5/6/7 Irradiation Disassembly and Metrology First Look
The Advanced Gas Reactor (AGR) Fuel Development and Qualification Program was established to perform research and development on tristructural isotropic (TRISO)-coated particle fuel to support deployment of high-temperature gas-cooled reactors (HTGRs), which are graphite-moderated nuclear reactors cooled with helium. This work continues as part of the Advanced Reactor Technologies (ART) TRISO Fuel Program. The overarching program goal is to provide a baseline fuel qualification data set to support licensing, deployment, and operation of HTGRs in the United States. To achieve these goals, the program includes fuel fabrication, irradiations of TRISO fuels and high-temperature materials (e.g., graphite), safety testing and post-irradiation examination (PIE), fuel performance modeling, and fission product transport and source term determination. The ART AGR program has conducted four distinct fuel irradiation experiments in the Advanced Test Reactor (ATR) at Idaho National Laboratory (INL). The first of these irradiation tests, designated AGR-1, began in ATR in December of 2006 and ended in November 2009. This experiment was primarily to act as a shakedown test of the multi-capsule test train design and to provide early data on fuel performance that would be used in fuel fabrication process development. AGR-1 fuel kernels were produced on an engineering scale, but the TRISO coatings and cylindrical fuel compacts were fabricated on a laboratory scale. The AGR-1 PIE was completed and the final report was published in 2015. The second irradiation test, AGR-2, started in ATR in June 2010 and ended in October 2013. The AGR-2 irradiation test was designed to provide fuel performance data for coated particles fabricated on an engineering-scale pilot line using a coater with an internal chamber diameter of 150 mm (6 in.). The final PIE report was published in 2021. AGR-3/4, a single irradiation that combined what were originally conceived as the third and fourth tests, was to support the refinement of fission product transport models and to assess the effects of sweep gas impurities on fuel performance and fission product transport. PIE of the AGR-3/4 experiment is still in progress as of this writing. The subject of this report is AGR-5/6/7, the final qualification test of AGR TRISO fuel made entirely at the engineering scale.
Tuning poly(N-isopropylacrylamide) surfactant phase behavior to access thermoresponsive micelle disassembly
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Kiwi-A prime disassembly
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Ball-and-Socket Joint Can Be Disassembled
Ball-and-socket joint originally developed for construction of large platforms in zero g could be used in such Earth-based temporary structures as scaffolding, camping equipment, tent posts, trade shows and displays. New joint consists of a socket mounted on central hub or union and ball-ended bolt or fitting mounted at end of a column or any structural member. Unit is self-contained, requires no loose hardware and is engaged or disengaged without tools manually, or remotely by a manipulator.
Detecting Cavitation Pitting Without Disassembly
Technique for detecting cavitation pitting in pumps, turbines, and other machinery uses low-level nuclear irradiation. Isotopes concentrated below surface emit gamma radiation, a portion of which is attenuated by overlying material. Where there are cavitation pits, output of gamma-ray detector fluctuates as detector is scanned near pits. Important to detect cavitation pits because nozzle, turbine blade, or other pump component weakened by cavitation could fail catastrophically and cause machine to explode.
Intracellular pH shift leads to microtubule assembly and microtubule-mediated motility during sea urchin fertilization - Correlations between elevated intracellular pH and microtubule activity and depressed intracellular pH and microtubule disassembly
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Design of an unmanned lunar cargo lander that reconfigures into a shelter for a habitation module or disassembles into parts useful to a permanent manned lunar base
NASA plans to establish a permanent manned lunar base by the first decade of the twenty-first century. It is extremely expensive to transport material from earth to the moon. Therefore, expense would be reduced if the vehicle that lands cargo on the moon could itself meet some of the material needs of establishing the lunar base. The design of a multi-functional lander that is entirely useful to the base after landing is described. Alternate designs of the overall lander configuration and possible uses of the lander and its components after landing are contained. The design solution is a lander employing the Saddlebagged Fuel Tank Configuration. After landing, its structure will be converted into a habitation module shelter that supports a protective layer of regolith. The fuel tanks will be cleaned and used as storage tanks for the lunar base. The engines and instrumentation will be saved as stock parts. Recommendations for further research and technology development to enhance future lander designs are given.
Lockwasher Strongly Resists Disassembly
Lockwasher designed to prevent counter-rotation and loosening of machine screw once screw tightened. Tabs engage slots in pawl-and-ratchet fashion. Features similar to those of "childproof" cap on pill bottle. Intended to replace cup-washer-and-screwhead combination exposed to high-speed, turbulent flow in turbomachinery.
Mechanical continuity and reversible chromosome disassembly within intact genomes removed from living cells
Chromatin is thought to be structurally discontinuous because it is packaged into morphologically distinct chromosomes that appear physically isolated from one another in metaphase preparations used for cytogenetic studies. However, analysis of chromosome positioning and movement suggest that different chromosomes often behave as if they were physically connected in interphase as well as mitosis. To address this paradox directly, we used a microsurgical technique to physically remove nucleoplasm or chromosomes from living cells under isotonic conditions. Using this approach, we found that pulling a single nucleolus or chromosome out from interphase or mitotic cells resulted in sequential removal of the remaining nucleoli and chromosomes, interconnected by a continuous elastic thread. Enzymatic treatments of interphase nucleoplasm and chromosome chains held under tension revealed that mechanical continuity within the chromatin was mediated by elements sensitive to DNase or micrococcal nuclease, but not RNases, formamide at high temperature, or proteases. In contrast, mechanical coupling between mitotic chromosomes and the surrounding cytoplasm appeared to be mediated by gelsolin-sensitive microfilaments. Furthermore, when ion concentrations were raised and lowered, both the chromosomes and the interconnecting strands underwent multiple rounds of decondensation and recondensation. As a result of these dynamic structural alterations, the mitotic chains also became sensitive to disruption by restriction enzymes. Ion-induced chromosome decondensation could be blocked by treatment with DNA binding dyes, agents that reduce protein disulfide linkages within nuclear matrix, or an antibody directed against histones. Fully decondensed chromatin strands also could be induced to recondense into chromosomes with pre-existing size, shape, number, and position by adding anti-histone antibodies. Conversely, removal of histones by proteolysis or heparin treatment produced chromosome decondensation which could be reversed by addition of histone H1, but not histones H2b or H3. These data suggest that DNA, its associated protein scaffolds, and surrounding cytoskeletal networks function as a structurally-unified system. Mechanical coupling within the nucleoplasm may coordinate dynamic alterations in chromatin structure, guide chromosome movement, and ensure fidelity of mitosis.
Joining, Disassembly, and Reconfiguration of Thermoplastic Composites for Space Applications
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Anti-Criticality Methods for Nuclear Thermal Rocket Launches and Their Associated Assembly / Disassembly in Space
When launching a nuclear reactor into space for use in a nuclear thermal rocket (NTR), safety to the public is of the outmost importance. Ensuring that the reactor will not go critical in an accident scenario is the biggest risk that must be overcome to protect the public. Use of anti-criticality devices and methods, such as the use of poison materials in the core or loading a select portion of the fuel on orbit, can prevent the reactor from going critical in any accident scenario. However, both methods (unpoisoning / loading fuel into the reactor core on orbit) will likely require the use of In Space Assembly and Manufacturing (ISAM) technology to remove the safeguards and ensure the reactor is fully operational before use. This paper discusses the use of ISAMs to help with the unpackaging of anti-criticality devices on orbit along with ISAMs ability to help verify and preform maintenance and inspection checks of the reactor system before operation and in between engine burns.