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At least 55 records · Page 3

Ab initio Potential-Energy Surfaces and Electron-Spin-Exchange Cross Sections for H-O2 Interactions

Accurate quartet- and doublet-state potential-energy surfaces for the interaction of a hydrogen atom and an oxygen molecule in their ground states have been determined from an ab initio calculation using large-basis sets and the internally contracted multireference configuration interaction method. These potential surfaces have been used to calculate the H-O2 electron-spin-exchange cross section; the square root of the cross section (in a(sub 0)), not taking into account inelastic effects, can be obtained approximately from the expressions 2.390E(sup -1/6) and 5.266-0.708 log10(E) at low and high collision energies E (in E(sub h)), respectively. These functional forms, as well as the oscillatory structure of the cross section found at high energies, are expected from the nature of the interaction energy. The mean cross section (the cross section averaged over a Maxwellian velocity distribution) agrees reasonably well with the results of measurements.

Stallcop, James R.↗

Gravitropism in a starchless mutant of Arabidopsis: implications for the starch-statolith theory of gravity sensing

The starch-statolith theory of gravity reception has been tested with a mutant of Arabidopsis thaliana (L.) Heynh. which, lacking plastid phosphoglucomutase (EC 2.7.5.1) activity, does not synthesize starch. The hypocotyls and seedling roots of the mutant were examined by light and electron microscopy to confirm that they did not contain starch. In upright wild-type (WT) seedlings, starch-filled plastids in the starch sheath of the hypocotyl and in three of the five columellar layers of the root cap were piled on the cell floors, and sedimented to the ceilings when the plants were inverted. However, starchless plastids of the mutant were not significantly sedimented in these cells in either upright or inverted seedlings. Gravitropism of light-grown seedling roots was vigorous: e.g., 10 degrees curvature developed in mutants rotated on a clinostat following a 5 min induction at 1 g, compared with 14 degrees in the WT. Curvatures induced during intervals from 2.5 to 30 min were 70% as great in the mutant as the WT. Thus under these conditions the presence of starch and the sedimentation of plastids are unnecessary for reception of gravity by Arabidopsis roots. Gravitropism by hypocotyls of light-grown seedlings was less vigorous than that by roots, but the mutant hypocotyls exhibited an average of 70-80% as much curvature as the WT. Roots and hypocotyls of etiolated seedlings and flower stalks of mature plants were also gravitropic, although in these cases the mutant was generally less closely comparable to the WT. Thus, starch is also unnecessary for gravity reception in these tissues.

NASA Program Space Biology↗

A 90GHz Bolometer Camera Detector System for the Green Bank Telescope

We describe a close-packed, two-dimensional imaging detector system for operation at 90GHz (3.3mm) for the 100 m Green Bank Telescope (GBT) This system will provide high sensitivity (<1mjy in 1s rapid imaging (15'x15' to 250 microJy in 1 hr) at the world's largest steerable aperture. The heart of this camera is an 8x8 close packed, Nyquist-sampled array of superconducting transition edge sensor bolometers. We have designed and are producing a functional superconducting bolometer array system using a monolithic planar architecture and high-speed multiplexed readout electronics. With an NEP of approx. 2.10(exp 17) W/square root Hz, the TES bolometers will provide fast linear sensitive response for high performance imaging. The detectors are read out by and 8x8 time domain SQUID multiplexer. A digital/analog electronics system has been designed to enable read out by SQUID multiplexers. First light for this instrument on the GBT is expected within a year.

Benford, Dominic J.↗

High Resolution VLBA Observations of NGC 4261

The VLBA's high angular resolution at 22 and 43 GHz allows the nuclei of nearby radio galaxies to be imaged with very high linear resolution (a few hundred Schwarzschild radii or less for central black hole masses of about 10(exp 8) - 10(exp 9) solar mass). This is the scale on which absorption by the dense ionized inner regions of nuclear accretion disk should be detected. We present 8, 22, and 43 GHz VLBA images of the two-sided radio jets in NGC 4261 (2C370) with sub-parsec resolution, and derive constraints on the physical properties of the inner accretion disk. In addition, preliminary results from multi-epoch VLBA observations to detect proper motions in the jet and counterjet, and thus determine the orientation of the radio axis to our line of sight, may be available. Our multi-frequency images show apparent absorption by a nearly edge-on disk at 8 GHz, but not evidence for absorption at 22 or 43 GHz. Assuming that this is due to a lower optical depth for free-free absorption at 22 and 43 GHz, we find an upper limit for the electron density of approximately 10(exp 8)* Square root of(0.1/L)/cubic cm, where L is the path length through the disk in pc. The disk inclination, and therefore the likely path length, will be better determined by the ongoing VLBA proper motions measurements. This research was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under contract with the National Aeronautics and Space Administration.

Jones, D. L.↗

Mechanotransduction molecules in the plant gravisensory response: amyloplast/statolith membranes contain a beta 1 integrin-like protein

It has been hypothesized that the sedimentation of amyloplasts within root cap cells is the primary event in the plant gravisensory-signal transduction cascade. Statolith sedimentation, with its ability to generate weighty mechanical signals, is a legitimate means for organisms to discriminate the direction of the gravity vector. However, it has been demonstrated that starchless mutants with reduced statolith densities maintain some ability to sense gravity, calling into question the statolith sedimentation hypothesis. Here we report on the presence of a beta 1 integrin-like protein localized inside amyloplasts of tobacco NT-1 suspension culture, callus cells, and whole-root caps. Two different antibodies to the beta 1 integrin, one to the cytoplasmic domain and one to the extracellular domain, localize in the vicinity of the starch grains within amyloplasts of NT-1. Biochemical data reveals a 110-kDa protein immunoprecipitated from membrane fractions of NT-1 suspension culture indicating size homology to known beta 1 integrin in animals. This study provides the first direct evidence for the possibility of integrin-mediated signal transduction in the perception of gravity by higher plants. An integrin-mediated pathway, initiated by starch grain sedimentation within the amyloplast, may provide the signal amplification necessary to explain the gravitropic response in starch-depleted cultivars.

NASA Discipline Plant Biology↗

A 90GHz Bolometer Camera Detector System for the Green

We describe a close-packed, two-dimensional imaging detector system for operation at 90GHz (3.3 mm) for the 100m Green Bank Telescope (GBT). This system will provide high sensitivity (less than 1mJy in 1s) rapid imaging (15'x15' to 150 micron Jy in 1 hr) at the world's largest steerable aperture. The heart of this camera is an 8x8 close-packed, Nyquist-sampled array of superconducting transition edge sensor (TES) bolometers. We have designed and are producing a functional superconducting bolometer array system using a monolithic planar architecture and high-speed multiplexed readout electronics. With an NEP of approximately 2 x 10(exp -17) W/square root of Hz, the TES bolometers will provide fast, linear, sensitive response for high performance imaging. The detectors are read out by an 8x8 time domain SQUID multiplexer. A digital/analog electronics system has been designed to enable read out by SQUID multiplexers. First light for this instrument on the GBT is expected within a year.

Benford, Dominic J.↗

Subcritical Growth of Electron Phase-Space Holes in Planetary Radiation Belts

The discovery of long-lived electrostatic coherent structures with large-amplitude electric fields (1 less than or equal to E less than or equal to 500 mV/m) by the Van Allen Probes has revealed alternative routes through which planetary radiation belts' acceleration can take place. Following previous reports showing that small phase-space holes, with q(phi)/T (exp c)(sub e) approximately minus 10 (exp -2) - 10 (exp -3), could result from electron interaction with large-amplitude whistlers, we demonstrate one possible mechanism through which holes can grow nonlinearly (i.e., Gamma alpha square root of phi) and subcritically as a result of momentum exchange between hot and cold electron populations. Our results provide an explanation for the common occurrence and fast growth of large-amplitude electron phase-space holes in the Earth's radiation belts.

Osmane, Adnane↗

Elevated levels of N-lauroylethanolamine, an endogenous constituent of desiccated seeds, disrupt normal root development in Arabidopsis thaliana seedlings

N-Acylethanolamines (NAEs) are prevalent in desiccated seeds of various plant species, and their levels decline substantially during seed imbibition and germination. Here, seeds of Arabidopsis thaliana (L.) Heynh. were germinated in, and seedlings maintained on, micromolar concentrations of N-lauroylethanolamine (NAE 12:0). NAE 12:0 inhibited root elongation, increased radial swelling of root tips, and reduced root hair numbers in a highly selective and concentration-dependent manner. These effects were reversible when seedlings were transferred to NAE-free medium. Older seedlings (14 days old) acclimated to exogenous NAE by increased formation of lateral roots, and generally, these lateral roots did not exhibit the severe symptoms observed in primary roots. Cells of NAE-treated primary roots were swollen and irregular in shape, and in many cases showed evidence, at the light- and electron-microscope levels, of improper cell wall formation. Microtubule arrangement was disrupted in severely distorted cells close to the root tip, and endoplasmic reticulum (ER)-localized green fluorescent protein (mGFP5-ER) was more abundant, aggregated and distributed differently in NAE-treated root cells, suggesting disruption of proper cell division, endomembrane organization and vesicle trafficking. These results suggest that NAE 12:0 likely influences normal cell expansion in roots by interfering with intracellular membrane trafficking to and/or from the cell surface. The rapid metabolism of NAEs during seed imbibition/germination may be a mechanism to remove this endogenous class of lipid mediators to allow for synchronized membrane reorganization associated with cell expansion.

Non-NASA Center↗

Investigation of Density Fluctuations in Supersonic Free Jets and Correlation with Generated Noise

The air density fluctuations in the plumes of fully-expanded, unheated free jets were investigated experimentally using a Rayleigh scattering based technique. The point measuring technique used a continuous wave laser, fiber-optic transmission and photon counting electronics. The radial and centerline profiles of time-averaged density and root-mean-square density fluctuation provided a comparative description of jet growth. To measure density fluctuation spectra a two-Photomultiplier tube technique was used. Crosscorrelation between the two PMT signals significantly reduced electronic shot noise contribution. Turbulent density fluctuations occurring up to a Strouhal number (Sr) of 2.5 were resolved. A remarkable feature of density spectra, obtained from the same locations of jets in 0.5< M<1.5 range, is a constant Strouhal frequency for peak fluctuations. A detailed survey at Mach numbers M = 0.95, 1.4 and 1.8 showed that, in general, distribution of various Strouhal frequency fluctuations remained similar for the three jets. In spite of the similarity in the flow fluctuation the noise characteristics were found to be significantly different. Spark schlieren photographs and near field microphone measurements confirmed that the eddy Mach wave radiation was present in Mach 1.8 jet, and was absent in Mach 0.95 jet. To measure correlation between the flow and the far field sound pressure fluctuations, a microphone was kept at a distance of 50 diameters, 30 deg. to the flow direction, and the laser probe volume was moved from point to point in the flow. The density fluctuations in the peripheral shear layer of Mach 1.8 jet showed significant correlation up to the measurement limit of Sr = 2.5, while for Mach 0.95 jet no correlation was measured. Along the centerline measurable correlation was found from the end of the potential core and at the low frequency range (Sr less than 0.5). Usually the normalized correlation values increased with an increase of the jet Mach number. The experimental data point out eddy Mach waves as a strong source of sound generation in supersonic jets and fail to locate the primary noise mechanism in subsonic jets.

Panda, J.↗

Intensity of primary cosmic-ray electrons of energy exceeding 8 GeV

Results are reported for measurement of the intensity and energy spectrum of primary cosmic-ray electrons with a spark-chamber-counter-emulsion detector flown at a mean altitude of 3 g/sq cm residual atmosphere. A least-squares fit to the flight data yields an electron spectrum from 8 to 80 GeV of approximately 93E to the -2.91 power electrons/sq m/sec per sr/GeV. The results are compared with those of previous experiments as well as with the spectrum obtained for galactic nonthermal radiation. It is concluded that a 'clumpy' magnetic field proportional to the square root of matter density is consistent with measurements of high-energy electrons and synchrotron radiation toward the center of the Galaxy, that a gradual steepening of the electron spectrum relative to the proton spectrum is consistent with an electron lifetime of 1 million years, and that the density of cosmic-ray nucleons and electrons should be essentially uniform throughout the Galaxy if the nucleons have the same lifetime as the electrons and if they traversed 4 to 5 g/sq cm in that lifetime.

Freier, P.↗

Defective secretion of mucilage is the cellular basis for agravitropism in primary roots of Zea mays cv. Ageotropic

Root caps of primary, secondary, and seminal roots of Z. mays cv. Kys secrete large amounts of mucilage and are in close contact with the root all along the root apex. These roots are strongly graviresponsive. Secondary and seminal roots of Z. mays cv. Ageotropic are also strongly graviresponsive. Similarly, their caps secrete mucilage and closely appress the root all along the root apex. However, primary roots of Z. mays cv. Ageotropic are non-responsive to gravity. Their caps secrete negligible amounts of mucilage and contact the root only at the extreme apex of the root along the calyptrogen. These roots become graviresponsive when their tips are coated with mucilage or mucilage-like materials. Peripheral cells of root caps of roots of Z. mays cv. Kys contain many dictyosomes associated with vesicles that migrate to and fuse with the plasmalemma. Root-cap cells of secondary and seminal (i.e. graviresponsive) roots of Z. mays cv. Ageotropic are similar to those of primary roots of Z. mays cv. Kys. However, root-cap cells of primary (i.e. non-graviresponsive) roots of Z. mays cv. Ageotropic have distended dictyosomal cisternae filled with an electron-dense, granular material. Large vesicles full of this material populate the cells and apparently do not fuse with the plasmalemma. Taken together, these results suggest that non-graviresponsiveness of primary roots of Z. mays cv. Ageotropic results from the lack of apoplastic continuity between the root and the periphery of the root cap. This is a result of negligible secretion of mucilage by cells along the edge of the root cap which, in turn, appears to be due to the malfunctioning of dictyosomes in these cells.

NASA Discipline Number 29-20↗

Modulation of the Jovian decametric radio emission by IO

It is argued that the sweeping of trapped energetic MeV protons by Io can result in induced emissions. The instability is caused by the density gradient occurring at the edge of the cavity. It is shown that the azimuthal Larmor drift of the energetic protons which is transverse to both the local magnetic field and the density gradient can excite extraordinary waves with frequencies slightly above the local electron cyclotron frequency. It is found that the emission coefficient is proportional to the square root of (n sub p/n sub t), where n sub p and n sub t denote the density of the energetic protons and the density of the thermal electrons, respectively. The theory also predicts a lower frequency cutoff. This cutoff frequency depends upon the density of thermal electrons in the source region.

Wu, C. S.↗

Measurement of dihedral angles by scanning electron microscopy.

The extension of Hoover's (1971) technique to the case of dihedral-angle measurement is described. Dihedral angles are often determined by interferometry on thermally grooved grain boundaries to obtain information on relative interfacial energies. In the technique considered the measured angles approach the true angles as the tilt angle approaches 90 deg. It is pointed out that the scanning electron microscopy method provides a means of seeing the real root of a groove at a lateral magnification which is higher than that obtainable with interferometry.

Achutaramayya, G.↗

Time course and auxin sensitivity of cortical microtubule reorientation in maize roots

The kinetics of MT [microtubule] reorientation in primary roots of Zea mays cv. Merit, were examined 15, 30, 45, and 60 min after horizontal positioning. Confocal microscopy of longitudinal tissue sections showed no change in MT orientation 15 and 30 min after horizontal placement. However, after 45 and 60 min, MTs of the outer 4-5 cortical cell layers along the lower side were reoriented. In order to test whether MT reorientation during graviresponse is caused by an auxin gradient, we examined the organization of MTs in roots that were incubated for 1 h in solutions containing 10(-9) to 10(-6) M IAA. IAA treatment at 10(-8) M or less showed no major or consistent changes but 10(-7) M IAA resulted in MT reorientation in the cortex. The auxin effect does not appear to be acid-induced since benzoic acid (10(-5) M) did not cause MT reorientation. The region closest to the maturation zone was most sensitive to IAA. The data indicate that early stages of gravity induced curvature occur in the absence of MT reorientation but sustained curvature leads to reoriented MTs in the outer cortex. Growth inhibition along the lower side of graviresponding roots appears to result from asymmetric distribution of auxin following gravistimulation.

NASA Discipline Plant Biology↗

Model system for studies of microbial dynamics at exuding surfaces such as the rhizosphere

An autoclavable all-glass system for studying microbial dynamics at permeable surfaces is described. Standard hydrophobic or hydrophilic membranes (46-mm diameter) of various pore sizes were supported on a glass frit through which nutrient solutions were pumped by a peristaltic pump. The pump provided a precisely controlled flow at speeds of 0.5 to 500 ml of defined or natural cell exudates per h, which passed through the membrane into a receiving vessel. The construction allowed a choice of membranes, which could be modified. The system was tested with a bacterium, isolated from rape plant roots (Brassica napus L.), that was inoculated on a hydrophilic membrane filter and allowed to develop into a biofilm. A defined medium with a composition resembling that of natural rape root exudate was pumped through the membrane at 0.5 ml/h. Scanning electron microscopic examinations indicated that the inoculum formed microcolonies embedded in exopolymers evenly distributed over the membrane surface. The lipid composition and content of poly-beta-hydroxybutyrate in free-living and adhered cells were determined by gas chromatography. The bacterial consumption of amino acids in the exudate was also studied.

NASA Discipline Number 61-10↗

On Convergence of Development Costs and Cost Models for Complex Spaceflight Instrument Electronics

Development costs of a few recent spaceflight instrument electrical and electronics subsystems have diverged from respective heritage cost model predictions. The cost models used are Grass Roots, Price-H and Parametric Model. These cost models originated in the military and industry around 1970 and were successfully adopted and patched by NASA on a mission-by-mission basis for years. However, the complexity of new instruments recently changed rapidly by orders of magnitude. This is most obvious in the complexity of representative spaceflight instrument electronics' data system. It is now required to perform intermediate processing of digitized data apart from conventional processing of science phenomenon signals from multiple detectors. This involves on-board instrument formatting of computational operands from row data for example, images), multi-million operations per second on large volumes of data in reconfigurable hardware (in addition to processing on a general purpose imbedded or standalone instrument flight computer), as well as making decisions for on-board system adaptation and resource reconfiguration. The instrument data system is now tasked to perform more functions, such as forming packets and instrument-level data compression of more than one data stream, which are traditionally performed by the spacecraft command and data handling system. It is furthermore required that the electronics box for new complex instruments is developed for one-digit watt power consumption, small size and that it is light-weight, and delivers super-computing capabilities. The conflict between the actual development cost of newer complex instruments and its electronics components' heritage cost model predictions seems to be irreconcilable. This conflict and an approach to its resolution are addressed in this paper by determining the complexity parameters, complexity index, and their use in enhanced cost model.

Kizhner, Semion↗

VLF wave emissions by pulsed and dc electron beams in space. II - Analysis of Spacelab 2 results

Results are presented from experiments on the generation of radio frequency waves in space plasmas by the injection of artificial electron beams into ionospheric plasmas that were conducted on the Spacelab 2 Space Shuttle mission. The observations of narrow-band radiation (Reeves et al., 1988) are compared with the prediction of a theory of wave generation by pulsed electron beams (Harker and Banks, 1987). Good agreement is found between the observations and predictions for the s = 0, root 2 solutions representing Cerenkov radiation with wave normal angles less than the Gendrin angle. Also, for broadband emissions, predictions using a single-ion cold plasma theory are compared with the observed features. The broadband dc and pulsed electron beam emissions also have features that can be explained in terms of the characteristics of whistler mode waves produced by Cerenkov resonance.

Reeves, G. D.↗

CO2 impact ionization-driven plasma instability observed by Pioneer Venus Orbiter at Periapsis

Observations of enhanced ac electric field noise about Pioneer Venus periapsis are shown to be related to spacecraft-generated impact ionization of the ambient CO2. The frequency of the electric field noise is found to peak in the vicinity of the CO2(+) ion plasma frequency and to closely follow the form of the neutral CO2 density profile. When the electric field noise in all channels is normalized by the square root of the CO2 number density, the ratio is constant. Since the impact electron density measured by the Pioneer Venus Langmuir probe, is observed to scale directly with the neutral CO2, the growth of the electric field amplitude is found to be linear in time with a growth rate proportional to the CO2(+) ion plasma frequency. On the basis of these results the impact ionization-driven instability is shown to be the ion acoustic instability. Implications for the lack of observations by Pioneer Venus of reflected-O(+)-driven instabilities, as have been proposed for the space shuttle, are discussed.

Curtis, S. A.↗