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NASA NTRS2001

Natural abundance deuterium and 18-oxygen effects on the precision of the doubly labeled water method

The doubly labeled water method for measuring total energy expenditure is subject to error from natural variations in the background 2H and 18O in body water. There is disagreement as to whether the variations in background abundances of the two stable isotopes covary and what relative doses of 2H and 18O minimize the impact of variation on the precision of the method. We have performed two studies to investigate the amount and covariance of the background variations. These were a study of urine collected weekly from eight subjects who remained in the Madison, WI locale for 6 wk and frequent urine samples from 14 subjects during round-trip travel to a locale > or = 500 miles from Madison, WI. Background variation in excess of analytical error was detected in six of the eight nontravelers, and covariance was demonstrated in four subjects. Background variation was detected in all 14 travelers, and covariance was demonstrated in 11 subjects. The median slopes of the regression lines of delta2H vs. delta18O were 6 and 7, respectively. Modeling indicated that 2H and 18O doses yielding a 6:1 ratio of final enrichments should minimize this error introduced to the doubly labeled water method.

NASA Discipline Regulatory Physiology↗

NASA NTRS2021

PURE mRNA display and cDNA display provide rapid detection of core epitope motif via high‐throughput sequencing

The reconstructed in vitro translation system known as the PURE system has been used in a variety of cell‐free experiments such as the expression of native and de novo proteins as well as various display methods to select for functional polypeptides. We developed a refined PURE‐based display method for the preparation of stable messenger RNA (mRNA) and complementary DNA (cDNA)‐peptide conjugates and validated its utility for in vitro selection. Our conjugate formation efficiency exceeded 40%, followed by gel purification to allow minimum carry‐over of components from the translation system to the downstream assay enabling clean and efficient random peptide sequence screening. We chose the commercially available anti‐FLAG M2 antibody as a target molecule for validation. Starting from approximately 1.7 × 10(exp 12) random sequences, a round‐by‐round high‐throughput sequencing showed clear enrichment of the FLAG epitope DYKDDD as well as revealing consensus FLAG epitope motif DYK(D/L/N)(L/Y/D/N/F)D. Enrichment of core FLAG motifs lacking one of the four key residues (DYKxxD) indicates that Tyr(Y) and Lys (K) appear as the two key residues essential for binding. Furthermore, the comparison between mRNA display and cDNA display method resulted in overall similar performance with slightly higher enrichment for mRNA display. We also show that gel purification steps in the refined PURE‐based display method improve conjugate formation efficiency and enhance the enrichment rate of FLAG epitope motifs in later rounds of selection especially for mRNA display. Overall, the generalized procedure and consistent performance of two different display methods achieved by the commercially available PURE system will be useful for future studies to explore the sequence and functional space of diverse polypeptides.

cDNA display, FLAG epitope, mRNA display, peptide ↗

NASA NTRSDate not supplied

Analysis of Igniter/Promoter Material Effects on Burn Length Variability in Astm G124 Standard Testing

ASTM G124 refers to the “Standard Test Method for Determining the Combustion Behavior of Metallic Materials in Oxygen Enriched Atmospheres”. Major test parameters are well defined in the standard and a detailed description of how to set up and conduct the test is also included. However, one variable - the igniter/promoter system - is not clearly restricted or specified. Due to the fact that this igniter/promoter system is not definitively specified, multiple materials have been used. This lack of specificity in igniter/promoter material was identified as a potential source of variability in test results, and as such has been selected for this study as a parameter to analyze and identify if the igniters/promoters should be standardized. For that purpose, several igniter/promoter systems have been selected (that were currently in use at various laboratories) which would be tested via ASTM G124 with Inconel 718 test rods. Testing was conducted over two phases – the first for screening promoter effects in the transition region of the material, and the second for comparing measured flammability thresholds identified through testing by each igniter/promoter type. The results of this study have found that igniter/promoter material is not a statistically significant factor in the variability of burn length in test samples. Initial results showing variability was likely due to small sample size, as the issue became less pronounced once more samples were tested and more data generated. Each of the igniter/promoter systems tested were effective at determining flammability thresholds and so it is concluded that no specific igniter/promoter needs to be identified in the ASTM G124 test standard.

oxygen compatibility↗

NASA NTRS2014

Worldwide Weather Radar Imagery May Allow Substantial Increase in Meteorite Fall Recovery

Weather radar imagery is a valuable new technique for the rapid recovery of meteorite falls, to include falls which would not otherwise be recovered (e.g. Battle Mountain). Weather radar imagery reveals about one new meteorite fall per year (18 falls since 1998), using weather radars in the United States alone. However, an additional ~75 other nations operate weather radar networks according to the UN World Meteorological Organization (WMO). If the imagery of those radars were analyzed, the current rate of meteorite falls could be improved considerably, to as much as ~3.6 times the current recovery rate based on comparison of total radar areal coverage. Recently, the addition of weather radar imagery, seismometry and internet-based aggregation of eyewitness reports has improved the speed and accuracy of fresh meteorite fall recovery [e.g. 1,2]. This was demonstrated recently with the radar-enabled recovery of the Sutter's Mill fall [3]. Arguably, the meteorites recovered via these methods are of special scientific value as they are relatively unweathered, fresh falls. To illustrate this, a recent SAO/NASA ADS search using the keyword "meteorite" shows that all 50 of the top search results included at least one named meteorite recovered from a meteorite fall. This is true even though only ~1260 named meteorite falls are recorded among the >49,000 individual falls recorded in the Meteoritical Society online database. The US NEXRAD system used thus far to locate meteorite falls covers most of the United States' surface area. Using a WMO map of the world's weather radars, we estimate that the total coverage of the other ~75 national weather radar networks equals about 3.6x NEXRAD's coverage area. There are two findings to draw from this calculation: 1) For the past 16 years during which 18 falls are seen in US radar data, there should be an additional ~65 meteorite falls recorded in worldwide radar imagery. Also: 2) if all of the world's radar data could be analyzed, the rate of recovery of fresh meteorite falls can increase by as much as ~3.6x the current rate. The authors' experience to date indicates that the most effective course of action would be to have local meteorite research groups (outside of the US) form research consortia and develop a working relationship with their nation's weather bureau for access to data. These research consortia could utilize the same, proven methods used for US NEXRAD imagery, internet eyewitness report aggregation, seismometry analysis, etc. to locate meteorite falls. The consortia could then recover and analyze meteorite falls and enrich their own research efforts. It would be beneficial to conduct a global program to coordinate the development of methods and data tools, as well as to coordinate meteorite sample sharing and research. Perhaps an institution such as the Meteoritical Society could lead such an effort.

Fries, Marc↗

NASA NTRS1969

I-123 production by the Sb-121 /alpha, 2n/ I-123 method

Cyclotron technique for producing high purity iodine 123, and procedures for isolating iodine products and recovering enriched target material

Blue, J. W.↗

NASA NTRS2004

Microscale Distribution of Hydrogen Isotopes in Two Carbonaceous Chondrites

Hydrogen isotopes are highly variable among primitive meteorites and interplanetary dust particles (IDPs) [1, 2]. In particular, many primitive objects exhibit D (and N-15) enrichments, relative to terrestrial values, thought to represent partial preservation of presolar material fractionated in molecular clouds. However, the diversity of D/H ratios among IDPs and chondrites indicates a complex history of processing in the solar nebula and on meteorite parent bodies. Deconvolving this record requires identification and characterization of the carriers of D enrichments in different objects. Isotopic imaging has proven to be a powerful method to quantitatively explore the distribution of D/H ratios on a one to several m scale in IDPs [2-4] and the CR chondrite Renazzo [5, 6]. In this study, we have used ion imaging to explore the microscale D/H distribution of two carbonaceous chondrites, Tagish Lake (unique) and Al Rais (CR2). Previous D/H measurements (on a ~tens of microns scale) of Tagish Lake matrix fragments by Messenger [7] and Engrand et al. [8] have found different results, most likely related to the analytical techniques used. Previous work has also shown a large range of D/H ratios in CR chondrites, including very large variations on a scale of a few microns [5, 6, 9].

Young, A. F.↗

NASA NTRS2003

Promoted Ignition and Burning Tests of Stainless Steel in Flowing and Nonflowing Oxygen

The Industry-Sponsored Metals Combustion Test Program 96-1 was coordinated through Wendell Hull & Associates, Inc. on behalf of several contributing companies, and all design and testing was performed at the NASA White Sands Test Facility. Phase I of this test program studied the threshold pressure for self-sustained burning of various types and sizes of stain less steel rods in nonflowing oxygen, as observed in Standard Test Method for Determining the Combustion Behavior of Metallic Materials in Oxygen-Enriched Atmospheres (ASTM G 124-95). Phase II studied the ignition and propagation of burning of 316L stainless steel rods and pipe in flowing gaseous oxygen. The test sample configurations were chosen to replicate previous promoted ignition and burning tests as well as to represent geometries and cross-sectional thicknesses common in industrial piping applications. The gas pressw'es and velocities for the test matrix were selected to generally compare with CGA G-4.4 guidelines for the use of stain less steel in oxygen service. This paper summarizes the results from the Phase I nonflowing oxygen tests and presents in detail the results of the Phase II flowing oxygen tests. The maximum sample burn-length is shown as a function of test pressure in Phase 1 and also as a function of gas velocity in Phase IT. These results indicate that flowing oxygen, under the given test conditions, significantly affects maximum sample burn length as compared to nonflowing oxygen. Supplementary flowing oxygen test data on stainless steel rods from a follow-up test program are consistent with these results and are presented herein.

Forsyth, Elliot T.↗

NASA NTRSDate not supplied

Worswick Hot Springs: A Radioactive Hydrothermal Field Site.

Introduction: We report on a systematic characterization of the environmental conditions at Worswick Hot Springs, a hydrothermal system in Idaho, USA. Because localized “hot spots” of elevated radiation and biofilms are easily accessible, various biological studies of radiation resistance and biosignature formation are possible, making this fieldsite relevant for analog field studies that consider microbiology, geo-chemistry, and ionizing radiation. In addition to Worswick being a natural radiation biology laboratory that may also be relevant for space biology applications, we assert that these unusual environmental conditions may inform us about locations on Mars that are also enriched in radioactive elements and their poten-tial for hosting biosignatures. Methods: We carried out repeated temperature and radiation measurements at the same hot spring locations to observe the system over time (Figure 2). Radiation: A Bicron Micro Analyst micro-r-meter (Bicron NE, Saint-Gobain Industrial Ceramics, Inc.) capable of sensing x-rays and gamma-rays (0-5000 µR/hr), was used to gather radiation data at twenty-four locations around and above the region of the two main stream channels. Water Temperature: A digital hand-held infrared thermometer (Oakton WD-39642-00 Mini-Temp Tester) was used for all measurements. ICP-MS aqueous geochemistry: Water samples were analyzed at the ISU Center for Archaeology, Materials and Applied Spectroscopy (CAMAS). Results: We have discovered localized areas of elevated radiation that are approximately 4 to 5 times greater than background radiation, and we have ob-served that both radioactivity and temperature of the spring waters vary over time. ICP-MS reveal the presence of thorium and uranium, which are correlated with elevated radioactivity. Several point sources of elevated radioactivity have been identified in both Stream A and Stream B.

Analog↗

NASA NTRS2023

Modeling holes and voids in three dimensions using a single element within the Extended Finite Element framework

This paper introduces a highly efficient computational approach using the extended finite element method to model three-dimensional mechanics of holes and voids. The defect is encapsulated within a single element with a tailored exponential enrichment function that captures the behavior near the discontinuity boundary and can account for the pressure acting on the void’s surface through an equivalent nodal force vector. The method’s results were compared against analytical solutions and conventional finite element method results for problems involving single and multiple holes. The comparison demonstrated that the method provides accurate stress concentration estimates while adhering to computational constraints.

Adnan Shahriar↗

NASA NTRS1952

Calculation of Internal Pressures in the Fuel Tube of a Nuclear Reactor

General procedures for computing internal pressures in fuel tubes of nuclear reactors are described and the effects on the pressure of varying neutron flux, fissioning material, and operating temperatures are discussed. A general proof is given that during pile operation each fission product is monotonically increasing and therefore a maximum amount of all elements is present at the time of shit down. The post-shutdown build-up of elements that are held in check during pile operation because of their inordinately high capture cross sections is calculated quantitatively. An account of chemical interactions between the many fission-product elements and the resulting effect on the total pressure completes the discussion. The general methods are illustrated by calculations applied to a system consisting of 90 percent enriched U235 in the form of UO2 packed into a hollow metal cylinder or "pin", operating at a flux of 8 x 10(exp 14) at 2000 F. Calculations of the pressure inside a pin are made with and without a sodium metal heat-transfer additive. The bulk of the pressure is shown to depend on the four elements, xenon, krypton, rubidium, and cesium; the amount of free oxygen, however, was also significant. For a shutdown time of 10(exp 6) seconds, the pressure was about 100 atmospheres.

Rosenbaum, B. M.↗

NASA NTRS2011

Regolith-Derived Heat Shield for Planetary Body Entry and Descent System with In Situ Fabrication

In this paper we will discuss a new mass-efficient and innovative way of protecting high-mass spacecraft during planetary Entry, Descent & Landing (EDL). Heat shields fabricated in situ can provide a thermal-protection system (TPS) for spacecraft that routinely enter a planetary atmosphere. By fabricating the heat shield with space resources from regolith materials available on moons and asteroids, it is possible to avoid launching the heat-shield mass from Earth. Three regolith processing and manufacturing methods will be discussed: 1) oxygen & metal extraction ISRU processes produce glassy melts enriched in alumina and titania, processed to obtain variable density, high melting point and heat-resistance; 2) compression and sintering of the regolith yield low density materials; 3) in-situ derived high-temperature polymers are created to bind regolith particles together, with a lower energy budget.

Hogue, Michael D.↗

NASA NTRS1994

An HP Adaptive Discontinuous Galerkin Method for Hyperbolic Conservation Laws

This dissertation addresses various issues for model classes of hyperbolic conservation laws. The basic approach developed in this work employs a new family of adaptive, hp-version, finite element methods based on a special discontinuous Galerkin formulation for hyperbolic problems. The discontinuous Galerkin formulation admits high-order local approximations on domains of quite general geometry, while providing a natural framework for finite element approximations and for theoretical developments. The use of hp-versions of the finite element method makes possible exponentially convergent schemes with very high accuracies in certain cases; the use of adaptive hp-schemes allows h-refinement in regions of low regularity and p-enrichment to deliver high accuracy, while keeping problem sizes manageable and dramatically smaller than many conventional approaches. The use of discontinuous Galerkin methods is uncommon in applications, but the methods rest on a reasonable mathematical basis for low-order cases and has local approximation features that can be exploited to produce very efficient schemes, especially in a parallel, multiprocessor environment. The place of this work is to first and primarily focus on a model class of linear hyperbolic conservation laws for which concrete mathematical results, methodologies, error estimates, convergence criteria, and parallel adaptive strategies can be developed, and to then briefly explore some extensions to more general cases. Next, we provide preliminaries to the study and a review of some aspects of the theory of hyperbolic conservation laws. We also provide a review of relevant literature on this subject and on the numerical analysis of these types of problems.

Bey, Kim S.↗

NASA NTRS1993

High purity isotopically enriched Ge-70 and Ge-74 single crystals - Isotope separation, growth, and properties

Ge-70 and Ge-74 isotopes were successfully separated from natural Ge, and were zone purified. Several highly enriched, high purity Ge-70 and Ge-74 single crystals were grown by the vertical Bridgman method. The growth system was designed for reliable growth of low dislocation density, high purity Ge single crystals of about 4 g weight. A Ge-70 and a Ge-74 crystal were selected for complete characterization. In spite of the large surface-to-volume ratio of these ingots, both Ge-70 and Ge-74 crystals contain low electrically active chemical net-impurity concentrations of about 2 x 10 exp 12/cu cm, which is 2 orders of magnitude better than that of Ge-74 crystals previously grown by two different groups. Isotopic enrichment of the Ge-70 and the Ge-74 crystals is 96.3 percent and 96.8 percent, respectively. The residual donors and acceptors present in both crystals were identified as phosphorus and copper, respectively. In addition, less than 10 exp 11/cu cm Ga, Al, and In were found in the Ge-70 crystal.

Itoh, Kohei↗

NASA NTRS2014

Radiation Quality Effects on Transcriptome Profiles in 3-d Cultures After Particle Irradiation

In this work, we evaluate the differential effects of low- and high-LET radiation on 3-D organotypic cultures in order to investigate radiation quality impacts on gene expression and cellular responses. Reducing uncertainties in current risk models requires new knowledge on the fundamental differences in biological responses (the so-called radiation quality effects) triggered by heavy ion particle radiation versus low-LET radiation associated with Earth-based exposures. We are utilizing novel 3-D organotypic human tissue models that provide a format for study of human cells within a realistic tissue framework, thereby bridging the gap between 2-D monolayer culture and animal models for risk extrapolation to humans. To identify biological pathway signatures unique to heavy ion particle exposure, functional gene set enrichment analysis (GSEA) was used with whole transcriptome profiling. GSEA has been used extensively as a method to garner biological information in a variety of model systems but has not been commonly used to analyze radiation effects. It is a powerful approach for assessing the functional significance of radiation quality-dependent changes from datasets where the changes are subtle but broad, and where single gene based analysis using rankings of fold-change may not reveal important biological information. We identified 45 statistically significant gene sets at 0.05 q-value cutoff, including 14 gene sets common to gamma and titanium irradiation, 19 gene sets specific to gamma irradiation, and 12 titanium-specific gene sets. Common gene sets largely align with DNA damage, cell cycle, early immune response, and inflammatory cytokine pathway activation. The top gene set enriched for the gamma- and titanium-irradiated samples involved KRAS pathway activation and genes activated in TNF-treated cells, respectively. Another difference noted for the high-LET samples was an apparent enrichment in gene sets involved in cycle cycle/mitotic control. It is plausible that the enrichment in these particular pathways results from the complex DNA damage resulting from high-LET exposure where repair processes are not completed during the same time scale as the less complex damage resulting from low-LET radiation.

Patel, Z. S.↗

NASA NTRSDate not supplied

Blue-enriched Light Improves Alertness and Mood Following Abrupt Awakening from Slow Wave Sleep

INTRODUCTION: All crew on the International Space Station (ISS) have a sleep opportunity at the same time. Emergencies arising during this time require all crew members to be abruptly awoken from sleep and to be alert, ready to work as a team, and to perform safety-critical tasks soon after waking. However, crew may experience sleep inertia after waking, which is associated with reduced alertness, poor mood, and impaired performance, especially if woken from deep sleep (slow wave sleep, SWS). Light has been shown to improve alertness during sleep deprivation and circadian misalignment. In this study, we assessed the efficacy of blue-enriched light to improve alertness and mood immediately after waking from SWS, i.e., during the sleep inertia period. METHODS: Twelve participants kept a sleep schedule of 8.5 h for 5 nights and 5 h for one night prior to the overnight laboratory visit (confirmed by actigraphy). Participants went to bed at their scheduled habitual bedtime in the laboratory and were monitored by standard polysomnography. After at least 5 min of SWS, participants were awoken and exposed to either red ambient light (control) or blue-enriched light (light) for 1 h. During this time, participants completed a subjective scale of alertness (Karolinska Sleepiness Scale, KSS) and visual analogue scales (VAS) of mood at 2 min, 17 min, 32 min, and 47 min after waking. Following this sleep inertia measurement period, all lights were turned off and participants were allowed to return to sleep. They were then awoken again from their subsequent SWS period and exposed to the opposite condition (control or light). A linear mixed-effects model with fixed effects of condition, time, and condition*time and a random effect of participant was used to determine the impact of light across the testing period. An average of baseline responses (pre-sleep) was included as a covariate. RESULTS: Compared to the control condition, participants exposed to blue-enriched light reported feeling more alert (KSS: F1,77=4.955, p=.029; VASalert: F1,77=8.226, p=.005), more cheerful (VAScheerful: F1,77=8.615, p=.004), less depressed (VASdepressed: F1,77=4.649, p=.034), and less lethargic (VASlethargic: F1,77=5.652, p=.020). DISCUSSION: Exposure to blue-enriched light immediately after waking from SWS may help to improve subjective alertness and mood. Future analyses will explore whether these findings extend to effects on cognitive performance. This countermeasure to sleep inertia may be suitable for implementation to alert crew members during mid-sleep emergencies but requires further testing in field settings.

alertness↗

NASA NTRSDate not supplied

Rise and Shine: Using Light as a Countermeasure to Sleep Inertia

INTRODUCTION: Sleep inertia describes the phenomenon of sleepiness and poor performance experienced after waking from sleep. This period of impaired alertness and performance is of significant concern to workers who nap on shift, or work on-call and are required to perform safety-critical tasks soon after waking (e.g., emergency services, healthcare, military). Light has been shown to acutely improve alertness during sleep deprivation and circadian misalignment. In this study, we assessed the efficacy of blue-enriched light to improve alertness and mood immediately after waking from SWS, i.e., during the sleep inertia period. METHODS: Twelve participants kept a sleep schedule of 8.5 h for 5 nights and 5 h for one night prior to the overnight laboratory visit (confirmed by actigraphy). Participants went to bed at their scheduled habitual bedtime in the laboratory and were monitored by standard polysomnography. After at least 5 min of SWS, participants were awoken and exposed to either red ambient light (control) or blue-enriched light (light) for 1 h. During this time, participants completed a subjective scale of alertness (Karolinska Sleepiness Scale, KSS) and visual analogue scales (VAS) of mood at 2 min, 17 min, 32 min, and 47 min after waking. Following this sleep inertia measurement period, all lights were turned off and participants were allowed to return to sleep. They were then awoken again from their subsequent SWS period and exposed to the opposite condition (control or light). A linear mixed-effects model with fixed effects of condition, time, and condition*time and a random effect of participant was used to determine the impact of light across the testing period. An average of baseline responses (pre-sleep) was included as a covariate. RESULTS: Compared to the control condition, participants exposed to blue-enriched light reported feeling more alert (KSS: F1,77=4.955, p=.029; VASalert: F1,77=8.226, p=.005), more cheerful (VAScheerful: F1,77=8.615, p=.004), less depressed (VASdepressed: F1,77=4.649, p=.034), and less lethargic (VASlethargic: F1,77=5.652, p=.020). DISCUSSION: Exposure to blue-enriched light immediately after waking from SWS may help to improve subjective alertness and mood. Future analyses will explore whether these findings extend to effects on cognitive performance. This countermeasure to sleep inertia may be suitable for implementation to alert crew members during mid-sleep emergencies but requires further testing in field settings.

alertness↗

NASA NTRS2016

Improved ASTM G72 Test Method for Ensuring Adequate Fuel-to-Oxidizer Ratios

The ASTM G72/G72M-15 Standard Test Method for Autogenous Ignition Temperature of Liquids and Solids in a High-Pressure Oxygen-Enriched Environment is currently used to evaluate materials for the ignition susceptibility driven by exposure to external heat in an enriched oxygen environment. Testing performed on highly volatile liquids such as cleaning solvents has proven problematic due to inconsistent test results (non-ignitions). Non-ignition results can be misinterpreted as favorable oxygen compatibility, although they are more likely associated with inadequate fuel-to-oxidizer ratios. Forced evaporation during purging and inadequate sample size were identified as two potential causes for inadequate available sample material during testing. In an effort to maintain adequate fuel-to-oxidizer ratios within the reaction vessel during test, several parameters were considered, including sample size, pretest sample chilling, pretest purging, and test pressure. Tests on a variety of solvents exhibiting a range of volatilities are presented in this paper. A proposed improvement to the standard test protocol as a result of this evaluation is also presented. Execution of the final proposed improved test protocol outlines an incremental step method of determining optimal conditions using increased sample sizes while considering test system safety limits. The proposed improved test method increases confidence in results obtained by utilizing the ASTM G72 autogenous ignition temperature test method and can aid in the oxygen compatibility assessment of highly volatile liquids and other conditions that may lead to false non-ignition results.

Juarez, Alfredo↗

NASA NTRS2016

Improved ASTM G72 Test Method for Ensuring Adequate Fuel-to-Oxidizer Ratios

Juarez, Alfredo↗