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At least 55 records · Page 3

Climate-Induced Mortality of Siberian Pine and Fir in the Lake Baikal Watershed, Siberia

Siberian pine (Pinus sibirica) and fir (Abies sibirica) (so called ''dark needle conifers", DNC) showed decreased radial growth increment within the Lake Baikal watershed since the 1980s with increasing mortality recorded since the year 2000. Tree ring width was strongly correlated with vapor pressure deficit, aridity and root zone moisture. Water stress from droughts made trees more susceptible to insect attacks causing mortality in about 10% of DNC stands within the Lake Baikal watershed. Within Siberia DNC mortality increased in the southern part of the DNC range. Biogeographically, tree mortality was located within the DNC - forest-steppes transition. Tree mortality was significantly correlated with drought and soil moisture anomalies. Within the interior of the DNC range mortality occurred within relief features with high water stress risk (i.e., steep convex south facing slopes with shallow well-drained soils). In general, DNC mortality in Siberia was induced by increased aridity and severe drought (inciting factors) in synergy with biotic attacks (contributing factor). In future climate scenarios with predicted increase in aridity DNC could be eliminated from the southern part of its current range and will be replaced by drought-resistant conifers and broadleaf species (e.g., Larix sibirica, Pinus silvestris, and Betula pubescence).

Conifer Mortality↗

Climate-Induced Mortality of Siberian Pine and Fir in the Lake Baikal Watershed, Siberia

Siberian pine (Pinus sibirica) and fir (Abies sibirica) (so called "dark needle conifers", DNC) showed decreased radial growth increment within the Lake Baikal watershed since the 1980s with increasing mortality recorded since the year 2000. Tree ring width was strongly correlated with vapor pressure deficit, aridity and root zone moisture. Water stress from droughts made trees more susceptible to insect attacks causing mortality in about 10% of DNC stands within the Lake Baikal watershed. Within Siberia DNC mortality increased in the southern part of the DNC range. Biogeographically, tree mortality was located within the DNC - forest-steppes transition. Tree mortality was significantly correlated with drought and soil moisture anomalies. Within the interior of the DNC range mortality occurred within relief features with high water stress risk (i.e., steep convex south facing slopes with shallow well-drained soils). In general, DNC mortality in Siberia was induced by increased aridity and severe drought (inciting factors) in synergy with biotic attacks (contributing factor). In future climate scenarios with predicted increase in aridity DNC could be eliminated from the southern part of its current range and will be replaced by drought-resistant conifers and broadleaf species (e.g., Larix sibirica, Pinus silvestris, and Betula pubescence).

Viacheslav I Kharuk↗

Climate Change Risks to Global Forest Health: Emergence of Unexpected Events of Elevated Tree Mortality Worldwide

Recent observations of elevated tree mortality following climate extremes, like heat and drought, raise concerns about climate change risks to global forest health. We currently lack both sufficient data and understanding to identify whether these observations represent a global trend toward increasing tree mortality. In this work, we document events of sudden and unexpected elevated tree mortality following heat and drought events in ecosystems that previously were considered tolerant or not at risk of exposure. These events underscore the fact that climate change may affect forests with unexpected force in the future. We use the events as examples to highlight current difficulties and challenges for realistically predicting such tree mortality events and the uncertainties about future forest condition. Advances in remote sensing technology and greater availably of high-resolution data, from both field assessments and satellites, are needed to improve both understanding and prediction of forest responses to future climate change.

59 BASIC BIOLOGICAL SCIENCES↗

Recent California tree mortality portends future increase in drought-driven forest die-off

Vegetation tolerance to drought depends on an array of site-specific environmental and plant physiological factors. This tolerance is poorly understood for many forest types despite its importance for predicting and managing vegetation stress. We analyzed the relationships between precipitation variability and forest die-off in California's Sierra Nevada and introduce a new measure of drought tolerance that emphasizes plant access to subsurface moisture buffers. We applied this metric to California's severe 2012-2015 drought, and show that it predicted the patterns of tree mortality. We then examined future climate scenarios, and found that the probability of droughts that lead to widespread die-off increases threefold by the end of the 21st century. Our analysis shows that tree mortality in the Sierra Nevada will likely accelerate in the coming decades and that forests in the Central and Northern Sierra Nevada that largely escaped mortality in 2012-2015 are vulnerable to die-off.

54 ENVIRONMENTAL SCIENCES↗

Modeling the mechanisms of coastal vegetation dynamics and ecosystem responses to changing water levels

Coastal forests are increasingly experiencing mortality due to inundation by fresh- and seawater, leading to their replacement by marshes. These shifts alter vegetation composition, biogeochemical cycling, carbon storage, and hydrology. Using a hydraulically enabled ecosystem demography model (FATES-Hydro), we conducted numerical experiments to investigate the mechanisms behind inundation-driven forest loss and the ecosystem-scale consequences of forest-to-marsh transitions. We compared mortality processes and their effects across broadleaf and conifer trees at two coastal sites – Lake Erie (freshwater) and Chesapeake Bay (saline). Our simulations show that hydraulic failure, driven by root loss under prolonged flooding, is the primary mortality mechanism across both tree types and sites. Forest replacement by marsh reduced ecosystem-scale leaf area index (LAI), gross primary production (GPP), transpiration, and deep soil water uptake in conifer forests, while broadleaf forests experienced smaller changes due to lower initial LAI and greater marsh compensation. Marsh invasion occurred following canopy thinning driven by tree mortality. These findings suggest that, under similar root loss, hydraulic failure dominates coastal tree mortality regardless of species or water type, with denser forests experiencing stronger ecosystem impacts. Our study identifies key mortality mechanisms and offers testable hypotheses for future empirical studies on coastal vegetation change.

Ding, Junyan [Occidental College, Los Angeles, CA ↗

Tree mortality during long-term droughts is lower in structurally complex forest stands

Increasing drought frequency and severity in a warming climate threaten forest ecosystems with widespread tree deaths. Canopy structure is important in regulating tree mortality during drought, but how it functions remains controversial. Here, we show that the interplay between tree size and forest structure explains drought-induced tree mortality during the 2012-2016 California drought. Through an analysis of over one million trees, we find that tree mortality rate follows a “negative-positive-negative” piecewise relationship with tree height, and maintains a consistent negative relationship with neighborhood canopy structure (a measure of tree competition). Trees overshadowed by tall neighboring trees experienced lower mortality, likely due to reduced exposure to solar radiation load and lower water demand from evapotranspiration. Our findings demonstrate the significance of neighborhood canopy structure in influencing tree mortality and suggest that re-establishing heterogeneity in canopy structure could improve drought resiliency. Our study also indicates the potential of advances in remote-sensing technologies for silvicultural design, supporting the transition to multi-benefit forest management.

59 BASIC BIOLOGICAL SCIENCES↗

Attaining freshwater and estuarine-water soil saturation in an ecosystem-scale coastal flooding experiment

Abstract Coastal upland forests are facing widespread mortality as sea-level rise accelerates and precipitation and storm regimes change. The loss of coastal forests has significant implications for the coastal carbon cycle; yet, predicting mortality likelihood is difficult due to our limited understanding of disturbance impacts on coastal forests. The manipulative, ecosystem-scale Terrestrial Ecosystem Manipulation to Probe the Effects of Storm Treatments (TEMPEST) experiment addresses the potential for freshwater and estuarine-water disturbance events to alter tree function, species composition, and ecosystem processes in a deciduous coastal forest in MD, USA. The experiment uses a large-unit (2000 m 2 ), un-replicated experimental design, with three 50 m × 40 m plots serving as control, freshwater, and estuarine-water treatments. Transient saturation (5 h) of the entire soil rooting zone (0–30 cm) across a 2000 m 2 coastal forest was attained by delivering 300 m 3 of water through a spatially distributed irrigation network at a rate just above the soil infiltration rate. Our water delivery approach also elevated the water table (typically ~ 2 m belowground) and achieved extensive, low-level inundation (~ 8 cm standing water). A TEMPEST simulation approximated a 15-cm rainfall event and based on historic records, was of comparable intensity to a 10-year storm for the area. This characterization was supported by showing that Hurricane Ida’s (~ 5 cm rainfall) hydrologic impacts were shorter (40% lower duration) and less expansive (80% less coverage) than those generated through experimental manipulation. Future work will apply TEMPEST treatments to evaluate coastal forest resilience to changing hydrologic disturbance regimes and identify conditions that initiate ecosystem state transitions.

54 ENVIRONMENTAL SCIENCES↗

Assessing Forest Level Response to the Death of a Dominant Tree within a Premontane Tropical Rainforest

Small-scale treefall gaps are among the most important forms of forest disturbance in tropical forests. These gaps expose surrounding trees to more light, promoting rapid growth of understory plants. However, the effects of such small-scale disturbances on the distribution of plant water use across tree canopy levels are less known. To address this, we explored plant transpiration response to the death of a large emergent tree, Mortoniodendron anisophyllum Standl. & Steyerm (DBH > 220 cm; height ~40 m). Three suppressed, four mid-story, and two subdominant trees were selected within a 50 × 44 m premontane tropical forest plot at the Texas A&M Soltis Center for Research and Education located in Costa Rica. We compared water use rates of the selected trees before (2015) and after (2019) the tree gap using thermal dissipation sap flow sensors. Hemispherical photography indicated a 40% increase in gap fraction as a result of changes in canopy structure after the treefall gap. Micrometeorological differences (e.g., air temperature, relative humidity, and vapor pressure deficit (VPD)) could not explain the observed trends. Rather, light penetration, as measured by sensors within the canopy, increased significantly in 2019. One year after the tree fell, the water usage of trees across all canopy levels increased modestly (15%). Moreover, average water usage by understory trees increased by 36%, possibly as a result of the treefall gap, exceeding even that of overstory trees. These observations suggest the possible reallocation of water use between overstory and understory trees in response to the emergent tree death. With increasing global temperatures and shifting rainfall patterns increasing the likelihood of tree mortality in tropical forests, there is a greater need to enhance our understanding of treefall disturbances that have the potential to redistribute resources within forests.

54 ENVIRONMENTAL SCIENCES↗

Comparative genome analyses suggest a hemibiotrophic lifestyle and virulence differences for the beech bark disease fungal pathogens Neonectria faginata and Neonectria coccinea

Abstract Neonectria faginata and Neonectria coccinea are the causal agents of the insect-fungus disease complex known as beech bark disease (BBD), known to cause mortality in beech forest stands in North America and Europe. These fungal species have been the focus of extensive ecological and disease management studies, yet less progress has been made toward generating genomic resources for both micro- and macro-evolutionary studies. Here, we report a 42.1 and 42.7 mb highly contiguous genome assemblies of N. faginata and N. coccinea, respectively, obtained using Illumina technology. These species share similar gene number counts (12,941 and 12,991) and percentages of predicted genes with assigned functional categories (64 and 65%). Approximately 32% of the predicted proteomes of both species are homologous to proteins involved in pathogenicity, yet N. coccinea shows a higher number of predicted mitogen-activated protein kinase genes, virulence determinants possibly contributing to differences in disease severity between N. faginata and N. coccinea. A wide range of genes encoding for carbohydrate-active enzymes capable of degradation of complex plant polysaccharides and a small number of predicted secretory effector proteins, secondary metabolite biosynthesis clusters and cytochrome oxidase P450 genes were also found. This arsenal of enzymes and effectors correlates with, and reflects, the hemibiotrophic lifestyle of these two fungal pathogens. Phylogenomic analysis and timetree estimations indicated that the N. faginata and N. coccinea species divergence may have occurred at ∼4.1 million years ago. Differences were also observed in the annotated mitochondrial genomes as they were found to be 81.7 kb (N. faginata) and 43.2 kb (N. coccinea) in size. The mitochondrial DNA expansion observed in N. faginata is attributed to the invasion of introns into diverse intra- and intergenic locations. These first draft genomes of N. faginata and N. coccinea serve as valuable tools to increase our understanding of basic genetics, evolutionary mechanisms and molecular physiology of these two nectriaceous plant pathogenic species.

Salgado-Salazar, Catalina↗

Seawater exposure causes hydraulic damage in dying Sitka-spruce trees

Sea-level rise is one of the most critical challenges facing coastal ecosystems under climate change. Observations of elevated tree mortality in global coastal forests are increasing, but the mechanism of salinity-stress induced tree mortality is currently unknown. We monitored progressive mortality and associated gas exchange and hydraulic shifts in Sitka-spruce (Picea sitchensis) trees located within a salinity gradient under an ecosystem-scale change of seawater exposure in Washington State, USA. Percentage of live foliated crown (PLFC) decreased and tree mortality increased with increasing soil salinity during the study period. A strong reduction in gas exchange and xylem hydraulic conductivity (Ks) occurred during tree death, with an increase of the percentage loss of xylem conductivity (PLC) and turgor loss point (ptlp). Hydraulic and osmotic shifts reflected that hydraulic function declined from seawater exposure and dying trees were unable to support osmotic adjustment. Constrained gas exchange was strongly related to hydraulic damage at both stem and leaf levels. Significant correlations between foliar sodium (Na+) concentration and gas exchange and key hydraulic parameters (Ks, PLC and ptlp ) suggest that cellular injury related to the toxic effects of ion accumulation impacted the physiology of these dying trees. This study provides evidence of toxic effects on cellular function that become manifest in all aspects of plant functioning, leading to unfavourable osmotic and hydraulic conditions.

Zhang, Hongxia↗

Evolutionary constraints and climate variability jointly shape starch–sugar balance in woody plants

Nonstructural carbohydrates (NSC) buffer plants against carbon imbalances, yet their partitioning between storage and soluble pools remains elusive at global scales. Here, we compiled a dataset of starch to soluble sugar ratio (St : Su) for 308 woody species across 220 sites world-wide and introduce a dimensionless index that integrates storage and demand while minimizing methodological artifacts. St : Su was strongly associated with growth, identifying it as a key axis of carbon allocation. Foliage consistently exhibited lower St : Su than lignified organs, reflecting a division between transient and conservative pools. Conifers accumulated more starch in foliage but less in stems relative to angiosperms, while leaf habits and mycorrhizal associations further modulated organ-specific strategies. Contrary to expectation, foliar and root St : Su varied little among biomes, but stems exhibited higher ratios in tropical rainforests than in boreal or arid regions, reflecting differences in species composition and adaptive storage under disturbance. Phylogeny constrained stem storage, whereas climatic variability, rather than mean conditions, dominated allocation in leaves and roots. These findings establish St : Su as a robust functional trait linking allocation strategies, growth, and resilience, which can be used to improve vegetation model prediction of forest productivity and mortality under climate variability.

Li, Weibin [Lanzhou Univ. (China)] (ORCID:00000001↗

TARP Identifying Critical Thresholds for Acute Response of Plants and Ecosystems to Water Stress at Walker Branch Watershed, 2002-2005

This dataset contains data from a manipulative field study aimed at identifying critical thresholds for acute response of plants and ecosystems to water stress (TARP) that took place at Walker Branch Watershed in Oak Ridge, Tennessee from 2002-2005 (2002-06-20 to 2005-12-16). The study used understory tents for the removal of 100% of the growing-season throughfall and stem flow, to provide data on the impact of acute drought on mechanisms responsible for growth and mortality of deciduous forest canopy trees representative of common plant functional types (Liriodendron and Quercus). Through three years of manipulation (2003, 2004 and 2005; pretreatment measurements in 2002) various measures of tree response to surface moisture deficits were recorded including root and leaf traits, plant nonstructural carbohydrates status, hourly sapflow, basal area, and periodic observations of foliar photosynthesis and conductance. Additionally, environmental data such as air and soil temperature, soil water content, and soil matric potential were recorded. This dataset contains data 16 files in comma-separate (*.csv) format. Additional metadata are provided: 16 data dictionaries and a file-level metadata file in comma separate (*.csv) format and a user guide in PDF (*.pdf) format.

basal area↗

Drought–induced increase in tree mortality and corresponding decrease in the carbon sink capacity of Canada's boreal forests from 1970 to 2020

Canada's boreal forests, which occupy approximately 30% of boreal forests world wide, play an important role in the global carbon budget. However, there is lit tle quantitative information available regarding the spatiotemporal changes in the drought-induced tree mortality of Canada's boreal forests overall and their associ ated impacts on biomass carbon dynamics. Here, we develop spatiotemporally ex plicit estimates of drought-induced tree mortality and corresponding biomass carbon sink capacity changes in Canada's boreal forests from 1970 to 2020. We show that the average annual tree mortality rate is approximately 2.7%. Approximately 43% of Canada's boreal forests have experienced significantly increasing tree mortality trends (71% of which are located in the western region of the country), and these trends have accelerated since 2002. This increase in tree mortality has resulted in sig nificant biomass carbon losses at an approximate rate of 1.51±0.29 MgC ha -1 year -1 (95% confidence interval) with an approximate total loss of 0.46±0.09 PgC year -1 (95% confidence interval). Under the drought condition increases predicted for this century, the capacity of Canada's boreal forests to act as a carbon sink will be further reduced, potentially leading to a significant positive climate feedback effect

59 BASIC BIOLOGICAL SCIENCES↗

Tree mortality in a warming world: causes, patterns, and implications

This ERL focus collection has published 17 papers that have advanced our understanding of different dimensions of warming-induced tree mortality. Here we summarize these focus collection papers, organized by four topics related to tree mortality: pathogens, droughts/heat waves, fire/bark beetles, and teleconnections/air pollution. This focus collection illustrates a variety of methods in measuring and modeling tree-mortality, and adds significant new research findings into the scientific literature on tree mortality from hotter droughts. Some of these results also are useful for policymakers and forest managers in addressing amplified forest stress and tree mortality as a result of increasingly severe warming-induced climate and weather extremes.

54 ENVIRONMENTAL SCIENCES↗

Long-term patterns of post-fire harvest diverge among ownerships in the Pacific West, U.S.A.

Abstract Post-fire harvest (PFH) is a forest management practice designed to salvage value from burned timber, mitigate safety hazards from dead trees, reduce long-term fuels, and prepare sites for replanting. Despite public controversy and extensive ecological research, little is known about how much PFH occurs on private and public lands in the U.S. Pacific West, or how practices changed with shifting forest policy and increasing area burned over the last three decades. We mapped PFH across 2.2M burned hectares in California, Oregon, and Washington between 1986-2017 and used time series intervention analysis to compare trends in area, rate (% of burned area harvested), and mean patch size between private (0.5M ha) and federal (1.6M ha) forest land and across a gradient of burn severity. Harvest rates varied by ownership (4.9% federal, 18.6% private, 8.0% overall), and practices evolved and diverged over the study period. PFH area and rate declined across all ownerships in the mid-1990s during a period of reduced fire activity. As area burned increased between the early 2000s and late 2010s, PFH area rebounded and surpassed late-1980s levels, while rates remained relatively low. On federal lands, PFH practices shifted in the early-to-mid 1990s towards lower rates (10.3% to 3.8%) and smaller patches (6.0 to 3.3 ha), following policy changes and increased litigation. PFH rates on federal lands decreased at all levels of burn severity, with the largest decreases (6.2% to 1.2%) in forests with low tree mortality (i.e. fire refugia). Conversely, private PFH rates and mean patch sizes more than doubled in forests burned at very low-to-moderate severity. Our results highlight how PFH practices have shifted with policy, socio-economic pressure, and increasing area burned over 31 years in the Pacific West. A similar area of post-fire harvest is now dispersed over larger fires, with practices diverging substantially between ownerships.

Zuspan, Aaron (ORCID:0000000315833710)↗

Divergent trait controls on soluble sugars and starch underlie global strategies of tree carbohydrate storage

Nonstructural carbohydrate (NSC) stores buffer tree metabolism, osmotic regulation, and defense, thereby mediating tolerance and survival under climate extremes. Yet, the functional and evolutionary determinants of interspecific variation in NSC remain elusive, limiting understanding and prediction of forest carbon allocation and mortality under global change. Here, we present a cross-species synthesis of NSC concentrations across multiple organs for 281 woody species from 102 mixed forest communities worldwide, where we quantified species-specific deviations from community means to disentangle intrinsic trait effects from environmental and methodological variation. We found phylogenetic signals in NSC deviations, with coniferous gymnosperms and evergreen species consistently maintaining lower stem soluble sugars and starch concentrations than co-occurring angiosperms and deciduous species, respectively. A global pattern emerged where greater stomatal sensitivity to leaf water potential was associated with declines in the relative concentrations of both sugars and starch. In contrast, xylem hydraulic safety traits showed weak and organ-dependent relationships with NSC concentrations. Sugars increased with photosynthetic capacity and declined with wood density, whereas starch showed the reverse pattern, which aligned with the distinct functional-metabolic roles of sugars and starch. By integrating trait-based ecology with a community-centered framework, our study provides global evidence that stomatal regulation, photosynthetic capacity, specific leaf area, and wood density jointly govern interspecific NSC variation, through contrasting effects on sugars and starch. These are among the most broadly measured traits globally, thus the emergent carbohydrate–trait relationships can have broad applications toward understanding and predicting forest growth and survival under climate change.

tropic system↗

Recovery of Forest Structure Following Large-Scale Windthrows in the Northwestern Amazon

The dynamics of forest recovery after windthrows (i.e., broken or uprooted trees by wind) are poorly understood in tropical forests. The Northwestern Amazon (NWA) is characterized by a higher occurrence of windthrows, greater rainfall, and higher annual tree mortality rates (~2%) than the Central Amazon (CA). We combined forest inventory data from three sites in the Iquitos region of Peru, with recovery periods spanning 2, 12, and 22 years following windthrow events. Study sites and sampling areas were selected by assessing the windthrow severity using remote sensing. At each site, we recorded all trees with a diameter at breast height (DBH) ≥ 10 cm along transects, capturing the range of windthrow severity from old-growth to highly disturbed (mortality > 60%) forest. Across all damage classes, tree density and basal area recovered to >90% of the old-growth values after 20 years. Aboveground biomass (AGB) in old-growth forest was 380 (±156) Mg ha -1 . In extremely disturbed areas, AGB was still reduced to 163 (±68) Mg ha -1 after 2 years and 323 (± 139) Mg ha -1 after 12 years. This recovery rate is ~50% faster than that reported for Central Amazon forests. The faster recovery of forest structure in our study region may be a function of its higher productivity and adaptability to more frequent and severe windthrows. These varying rates of recovery highlight the importance of extreme wind and rainfall on shaping gradients of forest structure in the Amazon, and the different vulnerabilities of these forests to natural disturbances whose severity and frequency are being altered by climate change.

54 ENVIRONMENTAL SCIENCES↗

Forest responses to simulated elevated CO 2 under alternate hypotheses of size- and age-dependent mortality

Elevated atmospheric carbon dioxide (eCO 2 ) is predicted to increase growth rates of forest trees. The extent to which increased growth translates to changes in biomass is dependent on the turnover time of the carbon, and thus tree mortality rates. Size- or age-dependent mortality combined with increased growth rates could result in either decreased carbon turnover from a speeding up of tree life cycles, or increased biomass from trees reaching larger sizes, respectively. However, most vegetation models currently lack any representation of size- or age-dependent mortality and the effect of eCO 2 on changes in biomass and carbon turnover times is thus a major source of uncertainty in predictions of future vegetation dynamics. Using a reduced-complexity form of the vegetation demographic model the Functionally Assembled Terrestrial Ecosystem Simulator to simulate an idealised tropical forest, we find increases in biomass despite reductions in carbon turnover time in both size- and age-dependent mortality scenarios in response to a hypothetical eCO 2 -driven 25% increase in woody net primary productivity (wNPP). Carbon turnover times decreased by 9.6% in size-dependent mortality scenarios due to a speeding up of tree life cycles, but also by 2.0% when mortality was age-dependent, as larger crowns led to increased light competition. Increases in aboveground biomass (AGB) were much larger when mortality was age-dependent (24.3%) compared with size-dependent (13.4%) as trees reached larger sizes before death. In simulations with a constant background mortality rate, carbon turnover time decreased by 2.1% and AGB increased by 24.0%, however, absolute values of AGB and carbon turnover were higher than in either size- or age-dependent mortality scenario. The extent to which AGB increases and carbon turnover decreases will thus depend on the mechanisms of large tree mortality: if increased size itself results in elevated mortality rates, then this could reduce by about half the increase in AGB relative to the increase in wNPP.

59 BASIC BIOLOGICAL SCIENCES↗