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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 55 records · Page 3

Intracellular distribution and stability of a luminescent rhenium( I ) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

Optical fluorescence microscopy was used in conjunction with X-ray fluorescence microscopy to monitor the stability and intracellular distribution of the luminescent rhenium(I) complex fac -[Re(CO) 3 (phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex, in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence techniques. Furthermore, X-ray fluorescence also showed that the Re-I complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

ecospec v0.1.0

Bespoke software for segmenting plants in ecoFABs to monitor growth and health. Contains python libraries for image alignment, training neural networks and running inference.

Zwart, PetrusH [Lawrence Berkeley National Laborat↗

Hyperspectral segmentation of plants in fabricated ecosystems

Hyperspectral imaging provides a powerful tool for analyzing above-ground plant characteristics in fabricated ecosystems, offering rich spectral information across diverse wavelengths. This study presents an efficient workflow for hyperspectral data segmentation and subsequent data analytics, minimizing the need for user annotation through the use of ensembles of sparse mixed scale convolution neural networks. The segmentation process leverages the diversity of ensembles to achieve high accuracy with minimal labeled data, reducing labor-intensive annotation efforts. To further enhance robustness, we incorporate image alignment techniques to address spatial variability in the dataset. Downstream analysis focuses on using the segmented data for processing spectral data, enabling monitoring of plant health. This approach provides a scalable solution for spectral segmentation, and facilitates actionable insights into plant conditions in complex, controlled environments. Our results demonstrate the utility of combining advanced machine learning techniques with hyperspectral analytics for high-throughput plant monitoring.

Zwart, Petrus H.↗

Quantitative capabilities of STXM to measure spatially resolved organic volume fractions of mixed organic/inorganic particles

Scanning transmission X-ray microscopy coupled with near-edge X-ray absorption and fine structure (STXM-NEXAFS) spectroscopy can be used to characterize the morphology and composition of aerosol particles. Here, two inorganic/organic systems are used to validate the calculation of organic volume fraction (OVF) and determine the level of associated error by using carbon K-edge STXM data at 278, 285.4, 288.6, and 320 eV. Using the mixture of sodium chloride and sucrose as one system and ammonium sulfate and sucrose as another, three solutions were made with 10:1, 1:1, and 1:10 mass ratios (inorganic to organic). The OVFs of the organic-rich aerosols of both systems deviated from the bulk OVF by less than 1%, while the inorganic-rich aerosols deviated by approximately 1 %. Aerosols from the equal mass mixture deviated more (about 4 %) due to thick inorganic regions exceeding the linear range of Beer's law. These calculations were performed after checking the data for poor image alignment, defocusing issues, and particles too thick to be analyzed. The potential for systematic error in the OVF calculation was also tested by assuming the incorrect composition. There is a small (about 0.5 %) OVF difference if the organic is erroneously assumed to be adipic acid rather than the known organic, sucrose. A much larger difference (up to 25 %) is seen if sodium chloride is assumed instead of ammonium sulfate. These results show that the OVF calculations are fairly insensitive to the organic while being much more sensitive to the choice of inorganic.

47 OTHER INSTRUMENTATION↗

Broad band X-ray astronomical spectroscopy

The Broad Band X-Ray Telescope (BBXRT) experiment has been designed for high sensitivity, moderate resolution spectrophotometry of cosmic sources in the range 0.5 to 12 keV. Principal elements are two co-aligned imaging telescopes with cooled Si(Li) detectors at each focus. The mirror design uses an approximate geometry of tightly nested, conical, foil reflectors that allow a large filling factor (high throughput) at small grazing angles (high energy response). Each detector consists of a cluster of five elements defined with grooves on a single crystal. This arrangement affords some spatial resolution as well as a means of substantial background reduction. Shuttle borne measurements will typically be of 2000 s duration for sources with a flux of 10 to the -12 erg/sq cm-s.

Serlemitsos, P. J.↗

Geometric accuracy assessment of Landsat-4 Thematic Mapper p-tapes

An analysis was performed on the P-format computer compatible tapes for the Landsat-4 Thematic Mapper (TM) scene 40109-15140 acquired November 2, 1982 over Washington, D.C. and environs. Three tests of sensor geometry were undertaken: (1) band-to-band registration, (2) line-to-line registration between swaths, and (3) geometric correction of sensor and spacecraft/ephemeris characteristics. The band-to-band and line-to-line registration was measured at one hundred pixel spacings along a line using the phase correlation image alignment method developed by Kuglin and Hines and adapted to a one-dimensional FFT correlation technique. The sensor and spacecraft geometric calibration analysis was checked by identifying 75 ground control points in the TM scene and on 1:24,000 topographic maps. A least squares fit of the gaps was computed and the vector offsets of the residuals was tabulated and plotted.

Bryant, N. A.↗

An evaluation of preliminary Doppler global velocimetry measurements

A review of Doppler Global Velocimetry (DGV) data obtained during wind tunnel tests on a 75 degree swept delta wing was performed. High frequency variations observed in normalized data files are attributed to image alignment problems. Unfortunately, initial DGV velocity data compared poorly with baseline reference data. Nonlinear DGV system operation during the tests is the likely source of this problem. Corrected data compares much more favorably and suggests that DGV is a valid measurement technique. Future DGV investigations should include a method or means for monitoring laser frequency relative to the ALF transfer function behavior.

Miller, L. Scott↗

Optical testing

Optical testing is one of the most vital elements in the process of preparing an optical instrument for launch. Without well understood, well controlled, and well documented test procedures, current and future mission goals will be jeopardized. We should keep in mind that the reason we test is to provide an opportunity to catch errors, oversights, and problems on the ground, where solutions are possible and difficulties can be rectified. Consequently, it is necessary to create tractable test procedures that truly provide a measure of the performance of all optical elements and systems under conditions which are close to those expected in space. Where testing is not feasible, accurate experiments are required in order to perfect models that can exactly predict the optical performance. As we stretch the boundaries of technology to perform more complex space and planetary investigations, we must expand the technology required to test the optical components and systems which we send into space. As we expand the observational wavelength ranges, so must we expand our range of optical sources and detectors. As we increase resolution and sensitivity, our understanding of optical surfaces to accommodate more stringent figure and scatter requirements must expand. Only with research and development in these areas can we hope to achieve success in the ever increasing demands made on optical testing by the highly sophisticated missions anticipated over the next two decades. Technology assessment and development plan for surface figure, surface roughness, alignment, image quality, radiometric quantities, and stray light measurement are presented.

Wyant, James↗

Evaluation of Head-Worn Display Concepts for Commercial Aircraft Taxi Operations

Previous research has demonstrated that a Head-Up Display (HUD) can be used to enable more capacity and safer aircraft surface operations. This previous research also noted that the HUD exhibited two major limitations which hindered the full potential of the display concept: 1) the monochrome HUD format; and, 2) a limited, fixed field of regard. Full-color Head Worn Displays (HWDs) with very small sizes and weights are emerging to the extent that this technology may be practical for commercial and business aircraft operations. By coupling the HWD with a head tracker, full-color, out-the-window display concepts with an unlimited field-of-regard may be realized to improve efficiency and safety in surface operations. A ground simulation experiment was conducted at NASA Langley to evaluate the efficacy of head-worn display applications which may directly address the limitations of the HUD while retaining all of its advantages in surface operations. The simulation experiment used airline crews to evaluate various displays (HUD, HWD) and display concepts in an operationally realistic environment by using a Chicago, O Hare airport database. The results pertaining to the implications of HWDs for commercial business and transport aviation applications are presented herein. Overall HWD system latency was measured and found to be acceptable, but not necessarily optimal. A few occurrences of simulator sickness were noted while wearing the HWD, but overall there appears to be commercial pilot acceptability and usability to the concept. Many issues were identified which need to be addressed in future research including continued reduction in user encumbrance due to the HWD, and improvement in image alignment, accuracy, and boresighting.

Bailey, Randall E.↗

The Expansion of the Young Supernova Remnant 0509-68.7 (N103B)

We present a second epoch of Chandra observations of the Type Ia Large Magellanic Cloud supernova remnant(SNR) 0509-68.7 (N103B) obtained in 2017. When combined with the earlier observations from 1999, we have a17.4 year baseline with which we can search for evidence of the remnant's expansion. Although the lack of strongpoint source detections makes absolute image alignment at the necessary accuracy impossible, we can measure thechange in the diameter and the area of the remnant, and find that it has expanded by an average velocity of 4170(2860, 5450) km s1. This supports the picture of this being a young remnant; this expansion velocity correspondsto an undecelerated age of 850 years, making the real age somewhat younger, consistent with results from lightecho studies. Previous infrared observations have revealed high densities in the western half of the remnant, likelyfrom circumstellar material, so it is probable that the real expansion velocity is lower on that side of the remnantand higher on the eastern side. A similar scenario is seen in Kepler's SNR. N103B joins the rare class ofMagellanic Cloud SNRs with measured proper motions.

Williams, Brian J.↗

Genetic Algorithm Phase Retrieval for the Systematic Image-Based Optical Alignment Testbed

Phase retrieval requires calculation of the real-valued phase of the pupil fimction from the image intensity distribution and characteristics of an optical system. Genetic 'algorithms were used to solve two one-dimensional phase retrieval problem. A GA successfully estimated the coefficients of a polynomial expansion of the phase when the number of coefficients was correctly specified. A GA also successfully estimated the multiple p h e s of a segmented optical system analogous to the seven-mirror Systematic Image-Based Optical Alignment (SIBOA) testbed located at NASA s Marshall Space Flight Center. The SIBOA testbed was developed to investigate phase retrieval techniques. Tiphilt and piston motions of the mirrors accomplish phase corrections. A constant phase over each mirror can be achieved by an independent tip/tilt correction: the phase Conection term can then be factored out of the Discrete Fourier Tranform (DFT), greatly reducing computations.

Taylor, Jaime↗

Poster - 48: Clinical assessment of ExacTrac stereoscopic imaging of spine alignment for lung SBRT

Purpose: To evaluate the validity of using spine as a surrogate for tumor positioning with ExacTrac stereoscopic imaging in lung stereotactic body radiation therapy (SBRT). Methods: Using the Novalis ExacTrac x-ray system, 39 lung SBRT patients (182 treatments) were aligned before treatment with 6 degrees (6D) of freedom couch (3 translations, 3 rotations) based on spine matching on stereoscopic images. The couch was shifted to treatment isocenter and pre-treatment CBCT was performed based on a soft tissue match around tumor volume. The CBCT data were used to measure residual errors following ExacTrac alignment. The thresholds for re-aligning the patients based on CBCT were 3mm shift or 3° rotation (in any 6D). In order to evaluate the effect of tumor location on residual errors, correlations between tumor distance from spine and individual residual errors were calculated. Results: Residual errors were up to 0.5±2.4mm. Using 3mm/3° thresholds, 80/182 (44%) of the treatments required re-alignment based on CBCT soft tissue matching following ExacTrac spine alignment. Most mismatches were in sup-inf, ant-post, and roll directions which had larger standard deviations. No correlation was found between tumor distance from spine and individual residual errors. Conclusion: ExacTrac stereoscopic imaging offers a quick pre-treatment patient alignment. However, bone matching based on spine is not reliable for aligning lung SBRT patients who require soft tissue image registration from CBCT. Spine can be a poor surrogate for lung SBRT patient alignment even for proximal tumor volumes.

60 APPLIED LIFE SCIENCES↗

Enhancing Multiplet Alignment Measurements with Imaging

We demonstrate that measurements of the gravitational tidal field made with spectroscopic redshifts can be improved with information from imaging surveys. The average orientation of small groups of galaxies, or “multiplets” is correlated with large-scale structure and is used to measure the direction of tidal forces. Previously, multiplet intrinsic alignment has been measured in DESI using galaxies that have spectroscopic redshifts. The DESI Legacy Imaging catalog can be used to supplement multiplet catalogs. Our findings show that galaxy positions from the imaging catalog produce a measurement similar to the measurements made with only spectroscopic data. This demonstrates that imaging can improve our signal-to-noise ratio for multiplet alignment in DESI.

Cosmic Web↗

Inverting Image Data For Optical Testing And Alignment

Data from images produced by slightly incorrectly figured concave primary mirror in telescope processed into estimate of spherical aberration of mirror, by use of algorithm finding nonlinear least-squares best fit between actual images and synthetic images produced by multiparameter mathematical model of telescope optical system. Estimated spherical aberration, in turn, converted into estimate of deviation of reflector surface from nominal precise shape. Algorithm devised as part of effort to determine error in surface figure of primary mirror of Hubble space telescope, so corrective lens designed. Modified versions of algorithm also used to find optical errors in other components of telescope or of other optical systems, for purposes of testing, alignment, and/or correction.

Shao, Michael↗

EUV and Hard X-ray Hartmann Wavefront Sensing for Optical Metrology, Alignment and Phase Imaging

For more than 15 years, Imagine Optic have developed Extreme Ultra Violet (EUV) and X-ray Hartmann wavefront sensors for metrology and imaging applications. These sensors are compatible with a wide range of X-ray sources: from synchrotrons, Free Electron Lasers, laser-driven betatron and plasma-based EUV lasers to High Harmonic Generation. In this paper, we first describe the principle of a Hartmann sensor and give some key parameters to design a high-performance sensor. We also present different applications from metrology (for manual or automatic alignment of optics), to soft X-ray source optimization and X-ray imaging.

20 metrology↗

TU-D-209-03: Alignment of the Patient Graphic Model Using Fluoroscopic Images for Skin Dose Mapping

Purpose: The Dose Tracking System (DTS) was developed to provide realtime feedback of skin dose and dose rate during interventional fluoroscopic procedures. A color map on a 3D graphic of the patient represents the cumulative dose distribution on the skin. Automated image correlation algorithms are described which use the fluoroscopic procedure images to align and scale the patient graphic for more accurate dose mapping. Methods: Currently, the DTS employs manual patient graphic selection and alignment. To improve the accuracy of dose mapping and automate the software, various methods are explored to extract information about the beam location and patient morphology from the procedure images. To match patient anatomy with a reference projection image, preprocessing is first used, including edge enhancement, edge detection, and contour detection. Template matching algorithms from OpenCV are then employed to find the location of the beam. Once a match is found, the reference graphic is scaled and rotated to fit the patient, using image registration correlation functions in Matlab. The algorithm runs correlation functions for all points and maps all correlation confidences to a surface map. The highest point of correlation is used for alignment and scaling. The transformation data is saved for later model scaling. Results: Anatomic recognition is used to find matching features between model and image and image registration correlation provides for alignment and scaling at any rotation angle with less than onesecond runtime, and at noise levels in excess of 150% of those found in normal procedures. Conclusion: The algorithm provides the necessary scaling and alignment tools to improve the accuracy of dose distribution mapping on the patient graphic with the DTS. Partial support from NIH Grant R01-EB002873 and Toshiba Medical Systems Corp.

60 APPLIED LIFE SCIENCES↗

A novel approach to correct the coded aperture misalignment for fast neutron imaging

Aperture alignment is crucial for the diagnosis of neutron imaging because it has significant impact on the coding imaging and the understanding of the neutron source. In our previous studies on the neutron imaging system with coded aperture for large field of view, “residual watermark,” certain extra information that overlies reconstructed image and has nothing to do with the source is discovered if the peak normalization is employed in genetic algorithms (GA) to reconstruct the source image. Some studies on basic properties of residual watermark indicate that the residual watermark can characterize coded aperture and can thus be used to determine the location of coded aperture relative to the system axis. In this paper, we have further analyzed the essential conditions for the existence of residual watermark and the requirements of the reconstruction algorithm for the emergence of residual watermark. A gamma coded imaging experiment has been performed to verify the existence of residual watermark. Based on the residual watermark, a correction method for the aperture misalignment has been studied. A multiple linear regression model of the position of coded aperture axis, the position of residual watermark center, and the gray barycenter of neutron source with twenty training samples has been set up. Using the regression model and verification samples, we have found the position of the coded aperture axis relative to the system axis with an accuracy of approximately 20 μm. Conclusively, a novel approach has been established to correct the coded aperture misalignment for fast neutron coded imaging.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗