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NASA NTRS2016

Overview of Proposed ISRU Technology Development

ISRU involves any hardware or operation that harnesses and utilizes in-situ resources (natural and discarded) to create products and services for robotic and human exploration: Assessment of physical, mineral chemical, and volatile water resources, terrain, geology, and environment (orbital and local). Production of replacement parts, complex products, machines, and integrated systems from feedstock derived from one or more processed resources. Civil engineering, infrastructure emplacement, and structure construction using materials produced from in situ resources. Radiation shields, landing pads, roads, berms, habitats, etc. Generation and storage of electrical, thermal, and chemical energy with in situ derived materials. Solar arrays, thermal wadis, chemical batteries, etc. ISRU is a disruptive capability: Enables more affordable exploration than todays paradigm. Allows more sustainable architectures to be developed. Understand the ripple effect in the other Exploration Elements: MAV: propellant selection, higher rendezvous altitude (higher DV capable with ISRU propellants). EDL: significantly reduces required landed mass. Life Support: reduce amount of ECLSS closure, reduce trash mass carried through propulsive maneuvers. Power: ISRU drives electrical requirements, reactant and regeneration for fuel cells for landers, rovers, and habitat backup. Every Exploration Element except ISRU has some flight heritage (power, propulsion, habitats, landers, life support, etc.) ISRU will require a flight demonstration mission on Mars before it will be included in the critical path. Mission needs to be concluded at least 10 years before first human landed mission to ensure lessons learned can be incorporated into final design. ISRU Formulation team has generated a (still incomplete) list of over 75 technical questions on more than 40 components and subsystems that need to be answered before the right ISRU system will be ready for this flight demo.

Mars exploration↗

NASA NTRS1999

XV-15 Tiltrotor Low Noise Approach Operations

Acoustic data have been acquired for the XV- 15 tiltrotor aircraft performing approach operations for a variety of different approach profile configurations. This flight test program was conducted jointly by NASA, the U.S. Army, and Bell Helicopter Textron, Inc. (BHTI) in June 1997. The XV-15 was flown over a large area microphone array, which was deployed to directly measure the noise footprint produced during actual approach operations, The XV- 15 flew realistic approach profiles that culminated in IGE hover over a landing pad. Aircraft tracking and pilot guidance was provided by a Differential Global Positioning System (DGPS) and a flight director system developed at BHTT. Approach profile designs emphasized noise reduction while maintaining handling qualities sufficient for tiltrotor commercial passenger ride comfort and flight safety under Instrument Flight Rules (IFR) conditions. A discussion of the approach profile design philosophy is provided. Five different approach profiles are discussed in detail -- 3deg., 6 deg., and 9 deg. approaches, and two very different 3 deg. to 9 deg. segmented approaches. The approach profile characteristics are discussed in details, followed by the noise footprints and handling qualities. Sound exposure levels are also presented on an averaged basis and as a function of the sideline distance for a number of up-range distances from the landing point. A comparison of the noise contour areas is also provided. The results document the variation in tiltrotor noise due to changes in operating condition, and indicate the potential for significant noise reduction using the unique tiltrotor capability of nacelle tilt.

Conner, David↗

NASA NTRS1999

XV-15 Tiltrotor Low Noise Approach Operations

Acoustic data have been acquired for the XV-15 tiltrotor aircraft performing approach operations for a variety of different approach profile configurations. This flight test program was conducted jointly by NASA, the U.S. Army, and Bell Helicopter Textron, Inc. (BHTI) in June 1997. The XV-15 was flown over a large area microphone array, which was deployed to directly measure the noise footprint produced during actual approach operations. The XV-15 flew realistic approach profiles that culminated in IGE hover over a landing pad. Aircraft tracking and pilot guidance was provided by a Differential Global Positioning System (DGPS) and a flight director system developed at BHTI. Approach profile designs emphasized noise reduction while maintaining handling qualities sufficient for tiltrotor commercial passenger ride comfort and flight safety under Instrument Flight Rules (IFR) conditions. A discussion of the approach profile design philosophy is provided. Five different approach profiles are discussed in detail -- 3 deg., 6 deg., and 9 deg. approaches, and two very different 3 deg. to 9 deg. segmented approaches. The approach profile characteristics are discussed in detail, followed by the noise footprints and handling qualities. Sound exposure levels are also presented on an averaged basis and as a function of the sideline distance for a number of up-range distances from the landing point. A comparison of the noise contour areas is also provided. The results document the variation in tiltrotor noise due to changes in operating condition, and indicate the potential for significant noise reduction using the unique tiltrotor capability of nacelle tilt.

Conner, David A.↗

DOE OSTI2023

Evaluation of strategies to narrow the product chain-length distribution of microbially synthesized free fatty acids

The dominant strategy for tailoring the chain-length distribution of free fatty acids (FFA) synthesized by heterologous hosts is expression of a selective acyl-acyl carrier protein (ACP) thioesterase. However, few of these enzymes can generate a precise (greater than 90% of a desired chain-length) product distribution when expressed in a microbial or plant host. The presence of alternative chain-lengths can complicate purification in situations where blends of fatty acids are not desired. We report the assessment of several strategies for improving the dodecanoyl-ACP thioesterase from the California bay laurel to exhibit more selective production of medium-chain free fatty acids to near exclusivity. We demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) was an effective library screening technique for identification of thioesterase variants with favorable shifts in Journal Pre-proof chain-length specificity. This strategy proved to be a more effective screening technique than several rational approaches discussed herein. With this data, we isolated four thioesterase variants which exhibited a more selective FFA distribution over wildtype when expressed in the fatty acid accumulating E. coli strain, RL08. We then combined mutations from the MALDI isolates to generate BTE-MMD19, a thioesterase variant capable of producing free fatty acids consisting of 90% of C12 products. Of the four mutations which conferred a specificity shift, we noted that three affected the shape of the binding pocket, while one occurred on the positively charged acyl carrier protein landing pad. Lastly, we fused the maltose binding protein (MBP) from E. coli to the N – terminus of BTE-MMD19 to improve enzyme solubility and achieve a titer of 1.9 g per L of twelve-carbon fatty acids in a shake flask.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Data for Evaluation of Strategies to Narrow the Product Chain-Length Distribution of Microbially Synthesized Free Fatty Acids

The dominant strategy for tailoring the chain-length distribution of free fatty acids (FFA) synthesized by heterologous hosts is expression of a selective acyl-acyl carrier protein (ACP) thioesterase. However, few of these enzymes can generate a precise (greater than 90% of a desired chain-length) product distribution when expressed in a microbial or plant host. The presence of alternative chain-lengths can complicate purification in situations where blends of fatty acids are not desired. We report the assessment of several strategies for improving the dodecanoyl-ACP thioesterase from the California bay laurel to exhibit more selective production of medium-chain free fatty acids to near exclusivity. We demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) was an effective library screening technique for identification of thioesterase variants with favorable shifts in chain-length specificity. This strategy proved to be a more effective screening technique than several rational approaches discussed herein. With this data, we isolated four thioesterase variants which exhibited a more selective FFA distribution over wildtype when expressed in the fatty acid accumulating E. coli strain, RL08. We then combined mutations from the MALDI isolates to generate BTE-MMD19, a thioesterase variant capable of producing free fatty acids consisting of 90% of C12 products. Of the four mutations which conferred a specificity shift, we noted that three affected the shape of the binding pocket, while one occurred on the positively charged acyl carrier protein landing pad. Finally, we fused the maltose binding protein (MBP) from E. coli to the N – terminus of BTE-MMD19 to improve enzyme solubility and achieve a titer of 1.9 g per L of twelve-carbon fatty acids in a shake flask.

Conversion↗

DOE OSTI2026

Advances in genetic tools for metabolic engineering of non-conventional yeasts

Non-conventional yeasts are emerging as powerful alternatives to Saccharomyces cerevisiae for metabolic engineering, owing to their innate stress tolerance, broad substrate utilization, and distinctive metabolic capabilities. These attributes position them as promising chassis for producing biofuels, pharmaceuticals, and specialty chemicals. This review synthesizes recent advances in genetic toolkits for four such species—Pichia kudriavzevii (Issatchenkia orientalis), Starmerella bombicola, Debaryomyces hansenii, and Pachysolen tannophilus—highlighting progress across plasmid architectures (episomal and integrative), identification of autonomously replicating sequences and centromeric elements, and the development of safe-harbor genomic loci. We summarize promoter and terminator libraries enabling tunable expression, the expansion of auxotrophic and antifungal selection markers with recycling strategies, and the rapid adaptation of CRISPR-based systems (Cas9 and Cas12a) with optimized guide RNA expression, multiplex editing, and approaches that enhance homologous recombination (e.g., KU70/80 disruption). We also review landing-pad platforms for modular, repeated integrations and transposon-based tools (e.g., piggyBac) that facilitate multigene pathway assembly. Collectively, these innovations are accelerating design-build-test-learn cycles and enabling precise, scalable engineering of non-conventional yeasts. Remaining challenges—including limited species-specific episomal systems, variable transformation efficiencies, genome-stability concerns, and alternative codon usage—define clear priorities for future toolkit development. Together, these advances and open needs chart a path toward robust, sustainable biomanufacturing using diverse non-conventional yeast chassis.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2024

XA21-mediated resistance to Xanthomonas oryzae pv. oryzae is dose dependent

The rice receptor kinase XA21 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae ( Xoo ), the causal agent of rice bacterial blight disease. To investigate the relationship between the expression level of XA21 and resulting resistance, we generated independent HA-XA21 transgenic rice lines accumulating the XA21 immune receptor fused with an HA epitope tag. Whole-genome sequence analysis identified the T-DNA insertion sites in sixteen independent T0 events. Further, through quantification of the HA-XA21 protein and assessment of the resistance to Xoo strain PXO99 in six independent transgenic lines, we observed that XA21-mediated resistance is dose dependent. In contrast, based on the four agronomic traits quantified in these experiments, yield is unlikely to be affected by the expression level of HA-XA21 . These findings extend our knowledge of XA21-mediated defense and contribute to the growing number of well-defined genomic landing pads in the rice genome that can be targeted for gene insertion without compromising yield.

60 APPLIED LIFE SCIENCES↗

NASA NTRS1982

Work Station For Inverting Solar Cells

Final work station along walking-beam conveyor of solar-array assembly line turns each pretabbed solar cell over, depositing it back-side-up onto landing pad, which centers cell without engaging collector surface. Solar cell arrives at inverting work station collector-side-up with two interconnect tabs attached to collector side. Cells are inverted so that second soldering operation takes place in plain view of operator. Inversion protects collector from damage when handled at later stages of assembly.

Feder, H.↗

NASA NTRS1979

Flight test evaluation of a digital controller used in a VTOL automatic approach and landing system

As part of the NASA Langley Research Center's effort to develop technology for VTOL operation in the air transportation system in the late 1980's and beyond, research has been conducted aimed at developing digital controller design procedures. This paper describes the verification of one design procedure by the flight evaluation of an advanced digital control algorithm. The control algorithm, operating at 10 iterations per second, follows step guidance commands with zero steady state error and thus provides an autotrim capability for the nonlinear vehicle. Changes in vehicle dynamics are accounted for using a gain scheduling technique. This control algorithm is combined with sensor filters, a trajectory generator, and a closed loop guidance algorithm to form a VTOL autoland system. A CH-47 tandem rotor helicopter which contains a set of sensors, onboard digital flight computers and electro-hydraulic actuators is used in the evaluation. All software, except input-output routines, is coded in FORTRAN using floating point arithmetic and executed in the flight computer. This autoland system is exercised by automatically flying straight-in descending decelerating trajectories typical of VFR manual approaches to a predetermined landing pad.

Downing, D. R.↗

NASA NTRS1982

Lateral control system design for VTOL landing on a DD963 in high sea states

The problem of designing lateral control systems for the safe landing of VTOL aircraft on small ships is addressed. A ship model is derived. The issues of estimation and prediction of ship motions are discussed, using optimal linear linear estimation techniques. The roll motion is the most important of the lateral motions, and it is found that it can be predicted for up to 10 seconds in perfect conditions. The automatic landing of the VTOL aircraft is considered, and a lateral controller, defined as a ship motion tracker, is designed, using optimal control techniqes. The tradeoffs between the tracking errors and the control authority are obtained. The important couplings between the lateral motions and controls are demonstrated, and it is shown that the adverse couplings between the sway and the roll motion at the landing pad are significant constraints in the tracking of the lateral ship motions. The robustness of the control system, including the optimal estimator, is studied, using the singular values analysis. Through a robustification procedure, a robust control system is obtained, and the usefulness of the singular values to define stability margins that take into account general types of unstructured modelling errors is demonstrated. The minimal destabilizing perturbations indicated by the singular values analysis are interpreted and related to the multivariable Nyquist diagrams.

Bodson, M.↗

NASA NTRS1983

Investigation of image enhancement techniques for the development of a self-contained airborne radar navigation system

This study was devoted to an investigation of the feasibility of applying advanced image processing techniques to enhance radar image characteristics that are pertinent to the pilot's navigation and guidance task. Millimeter (95 GHz) wave radar images for the overwater (i.e., offshore oil rigs) and overland (Heliport) scenario were used as a data base. The purpose of the study was to determine the applicability of image enhancement and scene analysis algorithms to detect and improve target characteristics (i.e., manmade objects such as buildings, parking lots, cars, roads, helicopters, towers, landing pads, etc.) that would be helpful to the pilot in determining his own position/orientation with respect to the outside world and assist him in the navigation task. Results of this study show that significant improvements in the raw radar image may be obtained using two dimensional image processing algorithms. In the overwater case, it is possible to remove the ocean clutter by thresholding the image data, and furthermore to extract the target boundary as well as the tower and catwalk locations using noise cleaning (e.g., median filter) and edge detection (e.g., Sobel operator) algorithms.

Phatak, A. V.↗

NASA NTRS1998

XV-15 Tiltrotor Low Noise Terminal Area Operations

Acoustic data have been acquired for the XV-15 tiltrotor aircraft performing a variety of terminal area operating procedures. This joint NASA/Bell/Army test program was conducted in two phases. During Phase 1 the XV-15 was flown over a linear array of microphones, deployed perpendicular to the flight path, at a number of fixed operating conditions. This documented the relative noise differences between the various conditions. During Phase 2 the microphone array was deployed over a large area to directly measure the noise footprint produced during realistic approach and departure procedures. The XV-15 flew approach profiles that culminated in IGE hover over a landing pad, then takeoffs from the hover condition back out over the microphone array. Results from Phase 1 identify noise differences between selected operating conditions, while those from Phase 2 identify differences in noise footprints between takeoff and approach conditions and changes in noise footprint due to variation in approach procedures.

Conner, David A.↗

NASA NTRS2010

Research Plans for Improving Understanding of Effects of Very Low-Frequency Noise of Heavy Lift Rotorcraft

This report reviews the English-language technical literature on infrasonic and low-frequency noise effects; identifies the most salient effects of noise produced by a future large civil tiltrotor aircraft on crew, passengers, and communities near landing areas; and recommends research needed to improve understanding of the effects of such noise on passengers, crew, and residents of areas near landing pads.

Fidell, Sanford↗

NASA NTRS2010

2010 ESMD Faculty Fellowship Project

This slide presentation reviews is composed of 6 individual sections. The first is a introductory section that explains the Exploration Systems Mission Directorate (ESMD) Faculty Fellowship Project, the purpose of which is to prepare selected university faculty to work with senior design students to complete projects that have potential to contribute to NASA objectives. The following university presentations represent the chosen projects: (1) the use of Exploration Toolset for the Optimization of Launch and Space Systems (X-TOOLSS) to optimize the Lunar Wormbot design; (2) development of Hardware Definition Language (HDL) realization of ITU G.729 for FGPA; (3) cryogenic fluid and electrical quick connect system and a lunar regolith design; (4) Lunar Landing Pad development; and (5) Prognostics for complex systems.

Carmen, Christina L.↗

NASA NTRS2011

Experimental Testing and Modeling of a Pneumatic Regolith Delivery System for ISRU

Excavating and transporting planetary regolith are examples of surface activities that may occur during a future space exploration mission to a planetary body. Regolith, whether it is collected on the Moon, Mars or even an asteroid, consists of granular minerals, some of which have been identified to be viable resources that can be mined and processed chemically to extract useful by-products, such as oxygen, water, and various metals and metal alloys. Even the depleted "waste" material from such chemical processes may be utilized later in the construction of landing pads and protective structures at the site of a planetary base. One reason for excavating and conveying planetary regolith is to deliver raw regolith material to in-situ resource utilization (ISRU) systems. The goal of ISRU is to provide expendable supplies and materials at the planetary destination, if possible. An in-situ capability of producing mission-critical substances such as oxygen will help to extend the mission and its success, and will greatly lower the overall cost of a mission by either eliminating, or significantly reducing, the need to transport the same expendable materials from the Earth. In order to support the goals and objectives of present and future ISRU projects, NASA seeks technology advancements in the areas of regolith conveying. Such systems must be effective, efficient and provide reliable performance over long durations while being exposed to the harsh environments found on planetary surfaces. These conditions include contact with very abrasive regolith particulates, exposure to high vacuum or dry (partial) atmospheres, wide variations in temperature, reduced gravity, and exposure to space radiation. Regolith conveying techniques that combine reduced failure modes and low energy consumption with high material transfer rates will provide significant value for future space exploration missions to the surfaces of the moon, Mars and asteroids. Pneumatic regolith conveying has demonstrated itself to be a viable delivery system through testing under terrestrial and reduced gravity conditions in recent years. Modeling and experimental testing have been conducted at NASA Kennedy Space Center to study and advance pneumatic planetary regolith delivery systems in support of NASA's ISRU project. The goal of this work is to use the model to predict solid-gas flow patterns in reduced gravity environments for ISRU inlet gas line allowing the eductor inlet gas flow to vary and depend on the flow pattern developed at the eductor as inferred by the experimental observations.

Santiago-Maldonado, Edgardo↗

NASA NTRS2005

Results from the NASA Capability Roadmap Team for In-Situ Resource Utilization (ISRU)

On January 14, 2004, the President of the United States unveiled a new vision for robotic and human exploration of space entitled, "A Renewed Spirit of Discovery". As stated by the President in the Vision for Space Exploration (VSE), NASA must "... implement a sustained and affordable human and robotic program to explore the solar system and beyond " and ".. .develop new technologies and harness the moon's abundant resources to allow manned exploration of more challenging environments." A key to fulfilling the goal of sustained and affordable human and robotic exploration will be the ability to use resources that are available at the site of exploration to "live off the land" instead of bringing everything from Earth, known as In-Situ Resource Utilization (ISRU). ISRU can significantly reduce the mass, cost, and risk of exploration through capabilities such as: mission consumable production (propellants, fuel cell reagents, life support consumables, and feedstock for manufacturing & construction); surface construction (radiation shields, landing pads, walls, habitats, etc.); manufacturing and repair with in-situ resources (spare parts, wires, trusses, integrated systems etc.); and space utilities and power from space resources. On January 27th, 2004 the President's Commission on Implementation of U.S. Space Exploration Policy (Aldridge Committee) was created and its final report was released in June 2004. One of the report's recommendations was to establish special project teams to evaluate enabling technologies, of which "Planetary in situ resource utilization" was one of them. Based on the VSE and the commission's final report, NASA established fifteen Capability Roadmap teams, of which ISRU was one of the teams established. From Oct. 2004 to May 2005 the ISRU Capability Roadmap team examined the capabilities, benefits, architecture and mission implementation strategy, critical decisions, current state-of-the-art (SOA), challenges, technology gaps, and risks of ISRU for future human Moon and Mars exploration. This presentation will provide an overview of the ISRU capability, architecture, and implementation strategy examined by the ISRU Capability Roadmap team, along with a top-level review of ISRU benefits, resources and products of interest, and the current SOA in ISRU processes and systems. The presentation will also highlight the challenges of incorporating ISRU into future missions and the gaps in technologies and capabilities that need to be filled to enable ISRU.

Sanders, Gerald B.↗

NASA NTRS2012

Regolith Advanced Surface Systems Operations Robot (RASSOR)

Regolith is abundant on extra-terrestrial surfaces and is the source of many resources such as oxygen, hydrogen, titanium, aluminum, iron, silica and other valuable materials, which can be used to make rocket propellant, consumables for life support, radiation protection barrier shields, landing pads, blast protection berms, roads, habitats and other structures and devices. Recent data from the Moon also indicates that there are substantial deposits of water ice in permanently shadowed crater regions and possibly under an over burden of regolith. The key to being able to use this regolith and acquire the resources, is being able to manipulate it with robotic excavation and hauling machinery that can survive and operate in these very extreme extra-terrestrial surface environments. In addition, the reduced gravity on the Moon, Mars, comets and asteroids poses a significant challenge in that the necessary reaction force for digging cannot be provided by the robot's weight as is typically done on Earth. Space transportation is expensive and limited in capacity, so small, lightweight payloads are desirable, which means large traditional excavation machines are not a viable option. A novel, compact and lightweight excavation robot prototype for manipulating, excavating, acquiring, hauling and dumping regolith on extra-terrestrial surfaces has been developed and tested. Lessons learned and test results will be presented including digging in a variety of lunar regolith simulant conditions including frozen regolith mixed with water ice.

Mueller, Robert P.↗

NASA NTRS2013

Impact-Actuated Digging Tool for Lunar Excavation

NASA s plans for a lunar outpost require extensive excavation. The Lunar Surface Systems Project Office projects that thousands of tons of lunar soil will need to be moved. Conventional excavators dig through soil by brute force, and depend upon their substantial weight to react to the forces generated. This approach will not be feasible on the Moon for two reasons: (1) gravity is 1/6th that on Earth, which means that a kg on the Moon will supply 1/6 the down force that it does on Earth, and (2) transportation costs (at the time of this reporting) of $50K to $100K per kg make massive excavators economically unattractive. A percussive excavation system was developed for use in vacuum or nearvacuum environments. It reduces the down force needed for excavation by an order of magnitude by using percussion to assist in soil penetration and digging. The novelty of this excavator is that it incorporates a percussive mechanism suited to sustained operation in a vacuum environment. A percussive digger breadboard was designed, built, and successfully tested under both ambient and vacuum conditions. The breadboard was run in vacuum to more than 2..times the lifetime of the Apollo Lunar Surface Drill, throughout which the mechanism performed and held up well. The percussive digger was demonstrated to reduce the force necessary for digging in lunar soil simulant by an order of magnitude, providing reductions as high as 45:1. This is an enabling technology for lunar site preparation and ISRU (In Situ Resource Utilization) mining activities. At transportation costs of $50K to $100K per kg, reducing digging forces by an order of magnitude translates into billions of dollars saved by not launching heavier systems to accomplish excavation tasks necessary to the establishment of a lunar outpost. Applications on the lunar surface include excavation for habitats, construction of roads, landing pads, berms, foundations, habitat shielding, and ISRU.

Wilson, Jak↗