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NASA NTRS1988

The role of extraterrestrial phenomena in extinction

In the several years since the Alvarez report of anomalously high iridium concentrations at the Cretaceous-Tertiary boundary, evidence for the involvement of meteorite impacts in biological extinction has increased dramatically. Much more research will be needed, however, before meteorite impact is established as a general causal factor in extinction. Of ever greater long-term interest is the possibility that other extraterrestrial forces have had important influences on the evolution of life. To recognize the effects of such forces, it will be necessary to coordinate the research of astronomy and paleontology so that testable predictions can be formulated. It is possible that known, systematic changes in the Solar System or Galaxy have had effects on global biology and that these effects have been preserved in the paleontological record.

Non-NASA Center↗

NASA NTRS1986

Lunar nodal tide and distance to the moon during the Precambrian

The first direct determination of the lunar distance in the Precambrian is presented. A 23.3 + or - 0.3 yr periodicity preserved in 2500 Myr BP Australian banded iron formation is interpreted as reflecting the climatic influence of the lunar nodal tide, which has been detected with its modern 18.6-yr periodicity in some modern climate records. The lunar distance at 2500 Myr BP would then have been about 52 earth radii. The implied history of precambrian tidal friction is in accord with both the more recent paleontological evidence and the long-term stability of the lunar orbit. The length of the Milankovitch cycles that modulate the ice ages today also evolve with the earth-moon system. Their detection in the Precambrian sedimentary record would then permit an independent determination of the lunar distance.

Walker, J. C. G.↗

NASA NTRS2000

Morphology and Viability of Pleistocene Microbiota from the CRREL Permafrost Tunnel Near Fox, Alaska

The U. S. Army Cold Regions Research and Engineering Laboratory maintains the CRREL Permafrost Tunnel at Fox, Alaska (-10 miles north of Fairbanks.) The active microbial ecosystems and the cryopreserved anabiotic viable microorganisms and dead microbial remains and biomarkers frozen within the permafrost and ice of the CRREL Permafrost Tunnel are of direct relevance to Astrobiology. Microbial extremophiles from permafrost and ice provide information concerning where and how should we search for evidence of life elsewhere in the Cosmos. The permafrost and ice wedges of the Fox tunnel preserves a magnificent of record of Pliocene, Pleistocene and Holocene life on Earth spanning more than 2.5 million years. This record includes frozen fossil bacteria, archaea, algae, mosses, higher plants, insects and mammals. In this paper we present the preliminary results of studies of the morphology, ultramicrostructure and elemental distributions of Fox tunnel microbiota as determined in-situ by the Environmental Scanning Electron Microscope (ESEM) and the Field Emission Scanning Electron Microscope (FESEM) investigations. The long-term viability of cryopreserved microbiota and potential implications to Astrobiology will be discussed.

Hoover, Richard B.↗

NASA NTRS1980

Near-comet trajectory design

The Halley Flyby/Tempel 2 Rendezvous mission requires not only a new spacecraft design and a new propulsion system (the Solar Electric Propulsion System), but it also requires new approaches to the trajectory and the mission design. Because of the feebleness of Tempel 2 gravitation and because of the presence of various other competing forces, a realization and/or preservation of a spacecraft trajectory about the comet is characteristically different from that about a more massive body. This paper surveys, compares, and evaluates the significance of various forces acting on the spacecraft in the vicinity of the comet. The range of variations and uncertainties inherent in modeling of these forces are also discussed. Then, a number of different types of trajectories relevant to the design of the Tempel 2 exploration are depicted and examined. These include short-arc trajectories, near-comet short- and long-term orbits, and comet-landing trajectories.

Jones, J. B.↗

NASA NTRS1994

Long river profiles, tectonism, and eustasy: A guide to interpreting fluvial terraces

Along three rivers at the Mendocino triple junction, northern California, strath, cut, and fill terraces have formed in response to tectonic and eustatic processes. Detailed surveying and radiometric dating at multiple sites indicate that lower reaches of the rivers are dominated by the effects of oscillating sea level, primarily aggradation and formation of fill terraces during sea level high stands, alternating with deep incision during low stands. A eustasy-driven depositional wedge extends tens of kilometers upstream on all rivers (tapering to zero thickness). This distance is greater than expected from studies of the effects of check dams on much smaller streams elsewhere, due in part to the large size of these rivers. However, the change in gradient is nearly identical to other base level rise studies: the depositional gradient is about half that of the original channel. Middle to upper reaches of each river are dominated by the effects of long-term uplift, primarily lateral and vertical erosion and formation of steep, unpaired strath terraces exposed only upstream of the depositional wedge. Vertical incision at a rate similar to that of uplift has occurred even during the present sea level high stand along rivers with highest uplift rates. Strath terraces have steeper gradients than the modern channel bed and do not merge with marine terraces at the river mouth; consequently, they cannot be used to determine altitudes of sea level high stands. Strath formation is a continuous process of response to long-term uplift, and its occurrence varies spatially along a river depending on stream power, and hence position, upstream. Strath terraces are found only along certain parts of a coastal stream: upstream of the aggradational effects of oscillating sea level, and far enough downstream that stream power is in excess of that needed to transport the prevailing sediment load. For a given size river, the greater the uplift rate, the greater the rate of vertical incision and, consequently, the less the likelihood of strath terrace formation and preservation.

Merritts, Dorothy J.↗

NASA NTRS1991

Interrelationships between continental freeboard, tectonics and mantle temperature

Oceanic hypsometry and isentropic melting models are combined to address the question of freeboard from the middle Archean to the present. In addition to the fraction of continental crust, the factors governing the long-term balance of the continental freeboard include the mantle potential temperature (TP), the oceanic lithosphere thickness, and the plate creation rate (C0). It is shown that variation in TP far outweighs the other factors in importance, with freeboard decreasing by 1 km for every TP increase of about 80 C. The huge ten- to thirtyfold increases in C0 backward in geological time that have been invoked to explain near-constant freeboard are shown to be unnecessary. The low value found for TP is consistent with the preservation of ancient diamonds in the deep South African lithosphere. It is concluded from this that the present cooling rate of the earth of about 46 C/Ga has general applicability over much of geological time.

Galer, S. J. G.↗

NASA NTRS1990

Mission to Planet Earth

To preserve the earth, it is necessary to understand the tremendously complex interactions of the atmosphere, oceans, land, and man's activities deeply enough to construct models that can predict the consequences of our actions and help us make sound environmental, energy, agriculture, and economic decisions. Mission to Planet Earth is NASA's suggested share and the centerpiece of the U.S. contribution to understanding the environment, the Global Change Research Program. The first major element of the mission would be the Earth Observing System, which would give the simultaneous, comprehensive, long-term earth coverage lacking previously. NASA's Geosynchronous Earth Observatory with two additional similar spacecraft would be orbited by the U.S., plus one each by Japan and the European Space Agency. These would be the first geostationary satellites to span all the disciplines of the earth sciences. A number of diverse data gathering payloads are also planned to be carried aboard the Polar Orbiting Platform. Making possible the long, continuous observations planned and coping with the torrent of data acquired will require technical gains across a wide front. Finally, how all this data is consolidated and disseminated by the EOS Data and Information System is discussed.

Tilford, Shelby G.↗

NASA NTRS1995

Urban remote sensing applications: TIMS observations of the City of Scottsdale

A research program has been initiated between Arizona State University and the City of Scottsdale, Arizona to study the potential applications of TIMS (Thermal Infrared Multispectral Scanner) data for urban scene classification, desert environmental assessment, and change detection. This program is part of a long-term effort to integrate remote sensing observations into state and local planning activities to improve decision making and future planning. Specific test sites include a section of the downtown Scottsdale region that has been mapped in very high detail as part of a pilot program to develop an extensive GIS database. This area thus provides excellent time history of the evolution of the city infrastructure, such as the timing and composition of street repavement. A second area of study includes the McDowell intensive study by state and local agencies to assess potential sites for urban development as well as preservation. These activities are of particular relevance as the Phoenix metropolitan area undergoes major expansion into the surrounding desert areas. The objectives of this study in urban areas are aimed at determining potential applications of TIMS data for classifying and assessing land use and surface temperatures. Land use centers on surface impermeability studies for storm runoff assessment and pollution control. These studies focus on determining the areal abundance of urban vegetation and undeveloped soil. Highly experimental applications include assessment and monitoring of pavement condition. Temperature studies focus on determining swimming pool area and temperature for use in monitoring evaporating and urban water consumption. These activities are of particular relevance as the Phoenix metropolitan area undergoes major expansion into the surrounding desert area.

Christensen, Philip R.↗

NASA NTRS2000

The State and Future of Mars Polar Science and Exploration

As the planet's principal cold traps, the martian polar regions have accumulated extensive mantles of ice and dust that cover individual areas of approx. 10(exp 6)sq km and total as much as 3-4 km thick. From the scarcity of superposed craters on their surface, these layered deposits are thought to he comparatively young-preserving a record of the seasonal and climatic cycling of atmospheric CO2, H2O, and dust over the past approx. 10(exp 5)-10(exp 8) years. For this reason, the martian polar deposits may serve as a Rosetta Stone for understanding the geologic and climatic history of the planet-documenting variations in insolation (due to quasiperiodic oscillations in the planet's obliquity and orbital elements), volatile mass balance, atmospheric composition, dust storm activity, volcanic eruptions, large impacts, catastrophic floods, solar luminosity, supernovae, and perhaps even a record of microbial life. Beyond their scientific value, the polar regions may soon prove important for another reason-providing a valuable and accessible reservoir of water to support the long-term human exploration of Mars. In this paper we assess the current state of Mars polar research, identify the key questions that motivate the exploration of the polar regions, discuss the extent to which current missions will address these questions, and speculate about what additional capabilities and investigations may be required to address the issues that remain outstanding.

Clifford, Stephen M.↗

NASA NTRS1994

The Large Impact Process Inferred from the Geology of Lunar Multiring Basins

The study of the geology of multiring impact basins on the Moon over the past ten years has given us a rudimentary understanding of how these large structures have formed and evolved on the Moon and other bodies. Two-ring basins on the Moon begin to form at diameters of about 300 km; the transition diameter at which more than two rings appear is uncertain, but it appears to be between 400 and 500 km in diameter. Inner rings tend to be made up of clusters or aligned segments of massifs and are arranged into a crudely concentric pattern; scarp-like elements may or may not be present. Outer rings are much more scarp-like and massifs are rare to absent. Basins display textured deposits, interpreted as ejecta, extending roughly an apparent basin radius exterior to the main topographic rim. Ejecta may have various morphologies, ranging from wormy and hummocky deposits to knobby surfaces; the causes of these variations are not known, but may be related to the energy regime in which the ejecta are deposited. Outside the limits of the textured ejecta are found both fields of satellitic craters (secondaries) and light plains deposits. Impact melt sheets are observed on the floors of relatively unflooded basins. Samples of impact melts from lunar basins have basaltic major-element chemistry, characterized by K, rare-earth elements (REE), P, and other trace elements of varying concentration (KREEP); ages are between 3.8 and 3.9 Ga. These lithologies cannot be produced through the fusion of known pristine (plutonic) rock types, suggesting the occurrence of unknown lithologies within the Moon. These melts were probably generated at middle to lower crustal levels. Ejecta compositions, preservation of pre-basin topography, and deposit morphologies all indicate that the excavation cavity of multiring basins is between about 0.4 and 0.6 times the diameter of the apparent crater diameter. Basin depths of excavation can be inferred from the composition of basin ejecta. A variety of mechanisms has been proposed to account for the formation of basin rings but none of them are entirely plausible. Mechanisms can be divided into two broad groups: (1) forcible uplift due to fluidization of the target; (2) concentric, brittle, fracturing and failure of the target, on regional (megaterraces) to global scales (lithospheric fracturing). Most basin rings are spaced at a constant factor on all planets. Evidence supports divergent ringforming models, so it may be that the ring-locating mechanism differs from the ring-forming mechanism. Thus, large-scale crustal foundering (megaterracing) could occur along concentric zones of weakness created by some type of resonant wave mechanism (fluidization and uplift); such immediate crustal adjustment could then be followed by long-term adjustment of the fractured lithosphere.

Spudis, Paul D.↗

NASA NTRS1995

Curation of Federally Owned Archeological Collections at NASA Langley Research Center

As a Federal agency, NASA has a moral and legal obligation to the public to manage the archeological heritage resources under its control. Archeological sites are unique, nonrenewable resources that must be preserved so that future generations may experience and interpret the material remains of the past. These sites are protected by a wide array of federal regulations. These regulations are intended to ensure that our nation's cultural heritage is preserved for the study and enjoyment of future generations. Once a site has been excavated, all that remains of it are the artifacts and associated records which, taken together, allow researchers to reconstruct the past. With the contextual information provided by associated records such as field notes, maps and photographs, archeological collections can provide important information about life in the past. An integral component of the federal archeology program is the curation of these databases so that qualified scholars will have access to them in years to come. Standards for the maintenance of archeological collections have been codified by various professional organizations and by the federal government. These guidelines focus on providing secure, climate-controlled archival storage conditions for the collections and an adequate study area in which researchers can examine the artifacts and documents. In the 1970's and early 1980's, a group of NASA employees formed the LRC Historical and Archeological Society (LRCHAS) in order to pursue studies of the colonial plantations that ha been displaced by Langley Research Center (LaRC). They collected data on family histories and land ownership as well as conducting archeological surveys and excavations at two important 17th-20th century plantation sites in LaRC, Cloverdale and Chesterville. The excavations produced a wealth of information in the form of artifacts, photographs, maps and other documents. Unfortunately, interest on the part of the LRCHAS membership waned before a report was written, and since 1982 the artifacts have moldered in a flimsy trailer with no climate controls, which had once served as a field laboratory but which threatened to become a tomb for the collection. A recent analysis of Langley's cultural resources by Gray & Pape, Inc. recommended that the collection be organized, cataloged, and placed in a proper curation facility in accordance with Federal regulations. The project for the LARSS program was to research curation standards, organize the collection, catalog it, and prepare it for transfer to a facility which could provide adequate long-term curation conditions for the artifacts and documents. The first phase was to organize the artifacts, which were lying about the lab in various stages of cleaning, analysis, and conservation. Once all of the artifacts from the various excavation units and levels had been regrouped, they were cleaned and/or repackaged in archivally-stable materials. A basic catalog was prepared which will provide interested parties with a rough idea of what we have and where it can be found. Another aspect of the project was to organize the records left by the LRCHAS. Bundles of papers, photographs, and field data found in every corner and drawer of the laboratory trailer were put into order and, where appropriate, copies were made on acid-free Permabond paper for long term storage. Finally, the entire collection and most of the lab equipment was transferred into a secure, climate controlled room which will serve as an archive and study space for qualified scholars interested in exploring LaRC's rich historical heritage.

Eastman, John Arnold↗

NASA NTRS1990

Solid rocket motor case insulation bond nondestructive evaluation

An effective insulator is needed to isolate the motor case from the hot combustion gases in such large solid rocket motors (SRMs) as those of the Space Shuttle, in order to preserve motor case integrity. An account is presently given of the ultrasonic NDE techniques employed to characterize the Space Shuttle's redesigned SRM. It is noted that current inspection procedures are sensitive to conditions that could result in anomalous ultrasonic indications; these conditions encompass (1) inner-outer surface nonparallelism, (2) sensor-surface nonparallelism, (3) contact pressure variation, (4) transducer coupling shoe wear, and (5) surface waviness. Both short-term and long-term NDE procedure recommendations are made.

Moore, Thomas K.↗

NASA NTRS1994

Escarpment evolution on high-elevation rifted margins: Insights derived from a surface processes model that combines diffusion, advection and reaction

Experiments with a surface processes model of large-scale (1-1000 km) long-term (1-100 m.y.) erosional denudation are used to establish the controls on the evolution of a model escarpment that is related to the rifting of a continent. The mdoel describes changes in topographic form as a result of sumultaneous short- and long-range mass transport representing hillslope (diffusive) processes and fluvial transport (advection), repsectively. Fluvial entrainment is modeled as a first-order kinetic reaction which reflects the erodibility of the substrate, and therefore the fluvial system is not necessarily carrying at capacity. One dimensional and planform models demonstrate that the principal controls on the evolution of an initially steep model escarpment are (1) antecedent topography/drainage; (2) the timesale (or equivalently a length scale) in the fluvial entrainment reaction; (3) the flexural response of the lithosphere to denudation; and (4) the relative efficiencies of the short- and long-range transport processes. When rainfall and substrate lithology are uniform, a significant amount of discharge draining over the escarpment top causes it to degrade. Only when the top of the model escarpment coincides with a drainage divide can escarpment retreat occur for these conditions. An additional requirement for retreat of a model escarpment without decline is a long reaction time scale for fluvial entrainment. This corresponds to a substrate that is hard to detach by flucial erosion, and therefore to fluvial erosion that is not transport limited. Coninuous backtilting of an escarpment due ot flexural isostatic uplift in response to denudational unloading helps maintain the scarp top as a divide. It is essntial if the escarpment gradient is to be preserved during retreat in a uniform lithology. Low flexural rigidieties propote steep and slowly retreating escarpments. For given rainfall and substrate conditions, the morphology of a retraeating model escarpment is determined by the ratio of hte short-range diffusive and long range advective transport efficiencies. A low ration (which is interpreted to correspond to a more humid, temperate climate) produces a convex upper slope, and concave lower slope morphology and only major escarpments are predicted to preserve a high scarp gradient. Lithological contrasts in hte model produce more complex morphologies and predict the formation of scarps crowned by an erosionaly resistant caprock. However, resistant caprocks are not an essential requirement for model scarps to retreat. We conclude that the inferred controls and model behavior are both consistent with the present-day morphology of rifted continental margins and with modern conceptual models of landscape evolution.

Kooi, Henk↗

NASA NTRS1998

Technology Assessment of High Capacity Data Storage Systems: Can We Avoid a Data Survivability Crisis

The density of digital storage media in our information-intensive society increases by a factor of four every three years, while the rate at which this data can be migrated to viable long-term storage has been increasing by a factor of only four every nine years. Meanwhile, older data stored on increasingly obsolete media, are at considerable risk. When the systems for which the media were designed are no longer serviced by their manufacturers (many of whom are out of business), the data will no longer be accessible. In some cases, older media suffer from a physical breakdown of components - tapes simply lose their magnetic properties after a long time in storage. The scale of the crisis is compatible to that facing the Social Security System. Greater financial and intellectual resources to the development and refinement of new storage media and migration technologies in order to preserve as much data as possible.

Halem, M.↗

NASA NTRS1999

Technology Assessment of High Capacity Data Storage Systems: Can We Avoid a Data Survivability Crisis?

In a recent address at the California Science Center in Los Angeles, Vice President Al Gore articulated a Digital Earth Vision. That vision spoke to developing a multi-resolution, three-dimensional visual representation of the planet into which we can roam and zoom into vast quantities of embedded geo-referenced data. The vision was not limited to moving through space, but also allowing travel over a time-line, which can be set for days, years, centuries, or even geological epochs. A working group of Federal Agencies, developing a coordinated program to implement the Vice President's vision, developed the definition of the Digital Earth as a visual representation of our planet that enables a person to explore and interact with the vast amounts of natural and cultural geo-referenced information gathered about the Earth. One of the challenges identified by the agencies was whether the technology existed that would be available to permanently store and deliver all the digital data that enterprises might want to save for decades and centuries. Satellite digital data is growing by Moore's Law as is the growth of computer generated data. Similarly, the density of digital storage media in our information-intensive society is also increasing by a factor of four every three years. The technological bottleneck is that the bandwidth for transferring data is only growing at a factor of four every nine years. This implies that the migration of data to viable long-term storage is growing more slowly. The implication is that older data stored on increasingly obsolete media are at considerable risk if they cannot be continuously migrated to media with longer life times. Another problem occurs when the software and hardware systems for which the media were designed are no longer serviced by their manufacturers. Many instances exist where support for these systems are phased out after mergers or even in going out of business. In addition, survivability of older media can suffer from physical breakdown of components (e.g. tapes simply lose their magnetic properties after a long time in storage). As a result, a potential data survivability crisis is emerging. The scale of the crisis is comparable to that facing the Social Security System. Sometime in one or two decades, the exponential growth of data will become so great that many enterprises will not be able to migrate through their data to more permanent media during the lifetime of the media on which it resides. This will result in significant losses of data and their resultant impacts. To avoid this crisis, we need to plan and devote greater financial and intellectual resources are needed for the development and refinement of new storage media and migration technologies in order to preserve all data any organization determines worth saving permanently. This talk will explore technological solutions and suggested recommendations to address this technological data crisis.

Halem, Milton↗

NASA NTRS2001

Driving Parameters for Distributed and Centralized Air Transportation Architectures

This report considers the problem of intersecting aircraft flows under decentralized conflict avoidance rules. Using an Eulerian standpoint (aircraft flow through a fixed control volume), new air traffic control models and scenarios are defined that enable the study of long-term airspace stability problems. Considering a class of two intersecting aircraft flows, it is shown that airspace stability, defined both in terms of safety and performance, is preserved under decentralized conflict resolution algorithms. Performance bounds are derived for the aircraft flow problem under different maneuver models. Besides analytical approaches, numerical examples are presented to test the theoretical results, as well as to generate some insight about the structure of the traffic flow after resolution. Considering more than two intersecting aircraft flows, simulations indicate that flow stability may not be guaranteed under simple conflict avoidance rules. Finally, a comparison is made with centralized strategies to conflict resolution.

Feron, Eric↗

NASA NTRS1994

Fluid Redistribution and Heart Rate in Humans During Whole-Body Tilting, G(z) Centrifugation, and Lower Body Negative Pressure

Gravity creates blood pressure gradients which redistribute body fluids towards the feet. Positive G(z) centrifugation and lower body negative pressure (LBNP) have been proposed to simulate these and other effects of gravity during long-term existence in microgravity. We hypothesized that the magnitude of upper-to-lower body fluid redistribution would increase according to the following order: short-arm centrifugation (SAC), long-arm centrifugation (LAC), head-up tilt (HUT), and LBNP. To test this hypothesis, we employed strain gauge plethysmography of the neck, thigh and calf during HUT and supine SAC and LAC up to lG(z) at the feet, and during supine LBNP to 100 mm Hg. Supine 100 mm Hg LBNP generates footward force and produces transmural blood pressures in the foot approximately equal to 1 G(z) (90 deg) HUT. Heart rate was measured via cardiotachometry. Control measurements were made while supine. SAC and LAC elicited similar increases in thigh volume at 1 G(z) (2.3 +/- 0.4 and 2.1 +/- 0.1%, respectively; mean +/- se, n greater than or equal to 7). At 100 mm Hg LBNP, thigh volume increased (3.4 +/- 0.3%) significantly more than during l G(z) centrifugation (p less than 0.05). Surprisingly, due to a paradoxical 0.6% reduction of thigh volume between 0.8 and 1.0 G(z) HUT, thigh volume was increased only 0.6 +/- 0.3% at 1 G(z) HUT. The calf demonstrated similar, although less definitive, responses to the various gravitational stimuli. Neck volume tended to decrease less during HUT than during the other stimuli. Heart rate increased similarly during HUT (18 +/- 2 beats/min) and LAC (12 +/- 2 beats/min), and exhibited still greater elevation during LBNP (29 +/- 4 beats/min), yet did not increase during SAC. These results suggest upright posture activates mechanisms that counteract footward fluid redistribution which are not activated during supine applications of simulated gravity. LAC more closely approximated effects of normal gravity (HUT) than LBNP. Therefore, when considering LBNP to simulate gravity, these findings support efforts to reduce the cardiovascular stress imposed by LBNP, while preserving the gravity-like force generated by LBNP.

Watenpaugh, D. E.↗

NASA NTRS1996

Comparison of Long-Term Storage in Chemical Fixatives on Morphology and Anatomy of Super-Dwarf Wheat

Wheat plants (Triticum aestivum L. cv Super-Dwarf) are grown in the microgravity of space and harvested for morphological and anatomical comparison with those exposed to gravity on earth. Such plants are subjected to relatively long periods of storage in chemical fixatives. Examination, evaluation and verification that the integrity of the vascular system is maintained for extended periods of storage in fixatives are required. McDowell and Trump's [4% Formaldehyde -- 1 % Glutaraldehyde (4F: 1 G)] or Variant I [(Russian Fixative): Formalin: Acetic Acid: Alcohol] fixatives, adjusted to pH 7.2, were placed in Aclam(TM), FilmORap(TM), or FilmOSun(TM) plastic bags on April 4, 1994. Wheat seedlings were harvested on days 9, 28, and 68 and preserved in these fixatives. Subsamples of leaves and/or seeds were taken from these stocks after various times in storage, dehydrated, and embedded in Spurr's, LR White's or Unicryl resin. Semithin (1 mm) and thin (50-70 nm) sections were examined by light and transmission electron microscopy. In a few sections, we have observed a slight plasmolysis of the cytostol in leaf tissue fixed with the Variant I, but overall there seem to be no major artifacts in the anatomical structure. The plasmalemma and other organelles appeared normal in the McDowell and Trump fixative. Use of differential chromophores suggests that LR White or Unicryl resins may give greater flexibility for enzyme localizations at both the light and electron microscopical levels.

Bubenheim, David L.↗