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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 55 records · Page 3
All sourcesNASA NTRSDOE OSTI
DOE OSTI2026

Introducing furanocoumarin biosynthetic genes in tomato results in coumarins accumulation and impacts growth

Over the past three decades, efforts to decipher plant metabolism have shed light on key enzymes driving specialized metabolite biosynthesis. Although only few pathways have been completely investigated to date, their characterization paves the way for exploring the potential effects of specialized metabolites on plant physiology. Among them is the linear furanocoumarin pathway, which was recently completed to produce up to psoralen. In this study, we report the first metabolic engineering of the linear furanocoumarin pathway to enable artificial psoralen production in tomato, through the integration of four genes coding for the enzymes: Umbelliferone Synthase (PsDiox), Demethylsuberosin Synthase (PsPT1), Marmesin Synthase (FcCYP76F112) and Psoralen Synthase (PsCYP71AJ3). Metabolic analyses confirmed the detection of small quantities of psoralen in the transgenic tomato line, but also highlighted a larger accumulation of coumarins and particularly scopoletin. Using morphophysiological and multi-omics analyses, we explorate how such metabolic modifications, could impact growth and affect plant physiology.

coumarins↗
DOE OSTI2023

Measuring calcium content in plants using NEXAFS spectroscopy

Calcium is important for the growth and development of plants. It serves crucial functions in cell wall and cell membrane structure and serves as a secondary messenger in signaling pathways relevant to nutrient and immunity responses. Thus, measuring calcium levels in plants is important for studies of plant biology and for technology development in food, agriculture, energy, and forest industries. Often, calcium in plants has been measured through techniques such as atomic absorption spectrophotometry (AAS), inductively coupled plasma–mass spectrometry (ICP-MS), and electrophysiology. These techniques, however, require large sample sizes, chemical extraction of samples or have limited spatial resolution. Here, we used near-edge X-ray absorption fine structure (NEXAFS) spectroscopy at the calcium L- and K-edges to measure the calcium to carbon mass ratio with spatial resolution in plant samples without requiring chemical extraction or large sample sizes. We demonstrate that the integrated absorbance at the calcium L-edge and the edge jump in the fluorescence yield at the calcium K-edge can be used to quantify the calcium content as the calcium mass fraction, and validate this approach with onion epidermal peels and ICP-MS. We also used NEXAFS to estimate the calcium mass ratio in hypocotyls of a model plant, Arabidopsis thaliana , which has a cell wall composition that is similar to that of onion epidermal peels. These results show that NEXAFS spectroscopy performed at the calcium edge provides an approach to quantify calcium levels within plants, which is crucial for understanding plant physiology and advancing plant-based materials.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS2018

Leaf Water Status Estimated from Visible-NIR Polarization Measurements

Remotely sensing the water status of plant canopies remains a long term goal of remote sensing research. Existing approaches to remotely sense canopy water status, such as the Crop Water Stress Index (CWSI) and the Equivalent Water Thickness (EWT), have limitations. The CWSI, based upon remotely sensing canopy radiant temperature in the thermal infrared spectral region, does not work well in humid regions, requires estimates of the vapor pressure deficit near the canopy during the remote sensing over-flight and, once stomata close, provides little information regarding the canopy water status. The EWT is based upon the physics of water-light interaction in the 900-2000nm spectral region, not plant physiology. Our goal is development of a remote sensing technique for estimating plant water status based upon measurements in the VIS/NIR spectral region, taking advantage of polarization physics. This technique would potentially provide remote sensing access to plant dehydration physiology – to the changes in cellular photochemistry and structure associated with water deficits in leaves. In this research, we used crossed optical polarization filters to measure the VIS/NIR light reflected from the leaf interior, R, as well as the leaf transmittance, T, for 78 corn (Zea mays) and soybean (Glycine max) leaves having relative water content (RWC) values between 0.60 and 0.98. Our results show that as RWC decreases R increases while T decreases. Our results tie R and T changes in the VIS/NIR to leaf physiological changes – linking the light scattered out of the drying leaf interior to its relative water content and to changes in leaf cellular structure and pigments.

Water↗
DOE OSTI2021

A physiological signal derived from sun-induced chlorophyll fluorescence quantifies crop physiological response to environmental stresses in the U.S. Corn Belt

Sun-induced chlorophyll fluorescence (SIF) measurements have shown unique potential for quantifying plant physiological stress. However, recent investigations found canopy structure and radiation largely control SIF, and physiological relevance of SIF remains yet to be fully understood. This study aims to evaluate whether the SIF-derived physiological signal improves quantification of crop responses to environmental stresses, by analyzing data at three different spatial scales within the U.S. Corn Belt, i.e. experiment plot, field, and regional scales, where ground-based portable, stationary and space-borne hyperspectral sensing systems are used, respectively. We found that, when controlling for variations in incoming radiation and canopy structure, crop SIF signals can be decomposed into non-physiological (i.e. canopy structure and radiation, 60% ~ 82%) and physiological information (i.e. physiological SIF yield, Φ F , 17% ~ 31%), which confirms the contribution of physiological variation to SIF. We further evaluated whether Φ F indicated plant responses under high-temperature and high vapor pressure deficit (VPD) stresses. The plot-scale data showed that Φ F responded to the proxy for physiological stress (partial correlation coefficient, r p = 0.40, p < 0.001) while non-physiological signals of SIF did not respond ( p > 0.1). The field-scale Φ F data showed water deficit stress from the comparison between irrigated and rainfed fields, and Φ F was positively correlated with canopy-scale stomatal conductance, a reliable indicator of plant physiological condition (correlation coefficient r = 0.60 and 0.56 for an irrigated and rainfed sites, respectively). The regional-scale data showed Φ F was more strongly correlated spatially with air temperature and VPD ( r = 0.23 and 0.39) than SIF ( r = 0.11 and 0.34) for the U.S. Corn Belt. The lines of evidence suggested that Φ F reflects crop physiological responses to environmental stresses with greater sensitivity to stress factors than SIF, and the stress quantification capability of Φ F is spatially scalable. Utilizing Φ F for physiological investigations will contribute to improve our understanding of vegetation responses to high-temperature and high-VPD stresses.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Impact of vegetation physiology and phenology on watershed hydrology in a semiarid watershed in the Pacific Northwest in a changing climate

Concurrent with changes in climate conditions, increases in atmospheric composition and transport can affect land surface processes by regulating physiological and phenological responses of plants, and consequently perturb water, carbon, and nitrogen (N) dynamics at multiple scales. Howerver, the relationships bwtween plants physiological and phenological and land surface processes in a changing climate are less well known due to the limitation of representing palnts properties in traditional hydrological models. In this study, we use the Community Land Model version 5 (CLM5) to investigate how projected changes in large scale atmospheric conditions can affect hydrologic and biogeochemical dynamics in the Upper Columbia-Priest Rapids (UCPR) watershed, a typical semi-arid watershed located in the north-western United States dominated by croplands. Six numerical experiments considering climate change, CO2 concentration, and N deposition consistent with the Representative Concentration Pathways (RCPs) 4.5 and 8.5 are performed at 1-km resolution over the period of 2015-2099. Our results show that, compared with historical results (1985-2014), the elevated CO2 directly increases plants N uptake rates that provide additional leaf N to enhance photosynthesis by the end of 21st century (2070-2099). The higher GPP leads to changes in stomatal resistance and increased leaf area index (LAI) that affect evaporatranspiration (ET) and its components. As a result of stomatal responses to a higher CO2 concentration, ET over corn fields declines by 24.6 mm yr-1 under RCP4.5, and by 64.0 mm yr-1 under RCP8.5, respectively by the end of the century from its historical mean of 428 mm yr-1. Meanwhile, ET over soybean fields increases by 27.7 mm yr-1 under RCP4.5 and 9.98 mm yr-1 under RCP8.5 from its historical mean of 507 mm yr-1 as a result of CO2 fertilization as soybeans are able to uptake more N to increase LAI significantly. Furtherfore, the changes in physiological and phenological in corn and soybean can strongly modulate water budget terms over the watershed in response to expected changes in greenhouse gas concentrations and atmospheric composition. Additionally, increases in CO2 concentration and N deposition are beneficial for improving water use efficiency of corn and soybean, therefore lead to less water consumption per unit gram of carbon produced from these crops. Our study elucidates the need to jointly consider effects of surface climate and atmospheric composition and transport in understanding and predicting watershed hydrologic and biogeochemical responses, especially for an agricultural watershed.

Zhu, Bowen↗
DOE OSTI2024

Non-destructive, whole-plant phenotyping reveals dynamic changes in water use efficiency, photosynthesis, and rhizosphere acidification of sorghum accessions under osmotic stress

Noninvasive phenotyping can quantify dynamic plant growth processes at higher temporal resolution than destructive phenotyping and can reveal phenomena that would be missed by end-point analysis alone. Additionally, whole-plant phenotyping can identify growth conditions that are optimal for both above- and below-ground tissues. However, noninvasive, whole-plant phenotyping approaches available today are generally expensive, complex, and non-modular. We developed a low-cost and versatile approach to noninvasively measure whole-plant physiology over time by growing plants in isolated hydroponic chambers. We demonstrate the versatility of our approach by measuring whole-plant biomass accumulation, water use, and water use efficiency every two days on unstressed and osmotically stressed sorghum accessions. We identified relationships between root zone acidification and photosynthesis on whole-plant water use efficiency over time. Our system can be implemented using cheap, basic components, requires no specific technical expertise, and should be suitable for any non-aquatic vascular plant species.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1986

The C23A system, an exmaple of quantitative control of plant growth associated with a data base

The architecture of the C23A (Chambers de Culture Automatique en Atmosphere Artificielles) system for the controlled study of plant physiology is described. A modular plant growth chambers and associated instruments (I.R. CO2 analyser, Mass spectrometer and Chemical analyser); network of frontal processors controlling this apparatus; a central computer for the periodic control and the multiplex work of processors; and a network of terminal computers able to ask the data base for data processing and modeling are discussed. Examples of present results are given. A growth curve analysis study of CO2 and O2 gas exchanges of shoots and roots, and daily evolution of algal photosynthesis and of the pools of dissolved CO2 in sea water are discussed.

Andre, M.↗
NASA NTRS1981

Life sciences flight experiments program - Overview

The considered LSFE program focuses on Spacelab life sciences missions planned for the 1984-1985 time frame. Life Sciences Spacelab payloads, launched at approximately 18-months intervals, will enable scientists to test hypotheses from such disciplines as vestibular physiology, developmental biology, biochemistry, cell biology, plant physiology, and a variety of other life sciences. An overview is presented of the LSFE program that will take advantage of the unique opportunities for biological experimentation possible on Spacelab. Program structure, schedules, and status are considered along with questions of program selection, and the science investigator working groups. A description is presented of the life sciences laboratory equipment program, taking into account the general purpose work station, the research animal holding facility, and the plant growth unit.

Berry, W. E.↗
DOE OSTI2020

Imaging of multiple fluorescent proteins in canopies enables synthetic biology in plants

Reverse genetics approaches have revolutionized plant biology and agriculture. Phenomics has the prospect of bridging plant phenotypes with genes, including transgenes, to transform agricultural fields. Genetically encoded fluorescent proteins (FPs) have revolutionized plant biology paradigms in gene expression, protein trafficking and plant physiology. While the first instance of plant canopy imaging of green fluorescent protein (GFP) was performed over 25 years ago, modern phenomics has largely ignored fluorescence as a transgene expression device despite the burgeoning FP colour palette available to plant biologists. Here, we show a new platform for stand-off imaging of plant canopies expressing a wide variety of FP genes. The platform-the fluorescence-inducing laser projector (FILP)-uses an ultra-low-noise camera to image a scene illuminated by compact diode lasers of various colours, coupled with emission filters to resolve individual FPs, to phenotype transgenic plants expressing FP genes. Each of the 20 FPs screened in plants were imaged at >3 m using FILP in a laboratory-based laser range. We also show that pairs of co-expressed fluorescence proteins can be imaged in canopies. The FILP system enabled a rapid synthetic promoter screen: starting from 2000 synthetic promoters transfected into protoplasts to FILP-imaged agroinfiltrated Nicotiana benthamiana plants in a matter of weeks, which was useful to characterize a water stress-inducible synthetic promoter. FILP canopy imaging was also accomplished for stably transformed GFP potato and in a split-GFP assay, which illustrates the flexibility of the instrument for analysing fluorescence signals in plant canopies.

abiotic stress↗
NASA NTRS1980

NASA-Ames Life Sciences Flight Experiments program - 1980 status report

The paper deals with the ESA's Spacelab LSFE (Life Sciences Flight Experiments) program which, once operational, will provide new and unique opportunities to conduct research into the effects of spaceflight and weightlessness on living organisms under conditions approximating ground-based laboratories. Spacelab missions, launched at 18-month intervals, will enable scientists to test hypotheses from such disciplines as vestibular physiology, developmental biology, biochemistry, cell biology, plant physiology, and similar life sciences.

Berry, W. E.↗
NASA NTRS1996

Gravity related features of plant growth behavior studied with rotating machines

Research in plant physiology consists mostly of studies on plant growth because almost everything a plant does is done by growing. Most aspects of plant growth are strongly influenced by the earth's gravity vector. Research on those phenomena address scientific questions specifically about how plants use gravity to guide their growth processes.

NASA Discipline Plant Biology↗
NASA NTRS2010

Using Leaf Chlorophyll to Parameterize Light-Use-Efficiency Within a Thermal-Based Carbon, Water and Energy Exchange Model

Chlorophylls absorb photosynthetically active radiation and thus function as vital pigments for photosynthesis, which makes leaf chlorophyll content (C(sub ab) useful for monitoring vegetation productivity and an important indicator of the overall plant physiological condition. This study investigates the utility of integrating remotely sensed estimates of C(sub ab) into a thermal-based Two-Source Energy Balance (TSEB) model that estimates land-surface CO2 and energy fluxes using an analytical, light-use-efficiency (LUE) based model of canopy resistance. The LUE model component computes canopy-scale carbon assimilation and transpiration fluxes and incorporates LUE modifications from a nominal (species-dependent) value (LUE(sub n)) in response to short term variations in environmental conditions, However LUE(sub n) may need adjustment on a daily timescale to accommodate changes in plant phenology, physiological condition and nutrient status. Day to day variations in LUE(sub n) were assessed for a heterogeneous corn crop field in Maryland, U,S.A. through model calibration with eddy covariance CO2 flux tower observations. The optimized daily LUE(sub n) values were then compared to estimates of C(sub ab) integrated from gridded maps of chlorophyll content weighted over the tower flux source area. The time continuous maps of daily C(sub ab) over the study field were generated by focusing in-situ measurements with retrievals generated with an integrated radiative transfer modeling tool (accurate to within +/-10%) using at-sensor radiances in green, red and near-infrared wavelengths acquired with an aircraft imaging system. The resultant daily changes in C(sub ab) within the tower flux source area generally correlated well with corresponding changes in daily calibrated LUE(sub n) derived from the tower flux data, and hourly water, energy and carbon flux estimation accuracies from TSEB were significantly improved when using C(sub ab) for delineating spatio-temporal variations in LUE(sub n). The results demonstrate the synergy between thermal infrared and shortwave reflective wavebands in producing valuable remote sensing data for operational monitoring of carbon and water fluxes.

Houlborg, Rasmus↗
DOE OSTI2025

The Ecological Impacts of Dry and Hot Shocks in the Land of Midnight Sun

Contreras-Serrano et al. (2025) and Heinzelmann et al. (2025) investigated how droughts and heatwaves affect plant physiology and community functions in the Arctic. At first glance, such studies may seem counterintuitive. As the Earth's refrigerator, the Arctic is probably the least likely place that one might associate with droughts and heatwaves. However, dry and hot shocks do occur there, and their frequency and intensity can be surprisingly high. On June 20, 2020, the thermometer at the weather station of the Siberian town of Verkhoyansk (67.55° N and 133.40° E) witnessed a record temperature of 38°C in the Article Circle (Ciavarella et al. 2021). This record Arctic temperature was the consequence of the congruence of the powers of the stratospheric polar vortex, the tropospheric jet stream, and the Arctic Oscillation, all of which reached record strength in the early months of 2020, priming the conditions for an unprecedented ecological disturbance (Overland and Wang 2021). This extreme event, now known as the 2020 Siberian heatwave, caused widespread wildfires, insect outbreaks, and permafrost degradation, releasing almost 60 million tons of carbon into the atmosphere (ESOTC 2020). Extreme weather events like this are not just limited to Siberia (Dobricic et al. 2020). The Arctic has warmed up much faster than the rest of the world, that is, “Arctic Amplification” (Moon et al. 2024). The total land area affected by severe, extreme, and very extreme heatwaves has doubled, tripled, and quadrupled, respectively, across the terrestrial Arctic since the 1950s, and the extent is projected to continue increasing throughout the 21st century (Rantanen et al. 2024).

Arctic↗
DOE OSTI2020

Changes in DNA Methylation in Response to 6-Benzylaminopurine Affect Allele-Specific Gene Expression in Populus Tomentosa

Cytokinins play important roles in the growth and development of plants. Physiological and photosynthetic characteristics are common indicators to measure the growth and development in plants. However, few reports have described the molecular mechanisms of physiological and photosynthetic changes in response to cytokinin, particularly in woody plants. DNA methylation is an essential epigenetic modification that dynamically regulates gene expression in response to the external environment. In this study, we examined genome-wide DNA methylation variation and transcriptional variation in poplar (Populus tomentosa) after short-term treatment with the synthetic cytokinin 6-benzylaminopurine (6-BA). We identified 460 significantly differentially methylated regions (DMRs) in response to 6-BA treatment. Transcriptome analysis showed that 339 protein-coding genes, 262 long non-coding RNAs (lncRNAs), and 15,793 24-nt small interfering RNAs (siRNAs) were differentially expressed under 6-BA treatment. Among these, 79% were differentially expressed between alleles in P. tomentosa, and 102,819 allele-specific expression (ASE) loci in 19,200 genes were detected showing differences in ASE levels after 6-BA treatment. Combined DNA methylation and gene expression analysis demonstrated that DNA methylation plays an important role in regulating allele-specific gene expression. To further investigate the relationship between these 6-BA-responsive genes and phenotypic variation, we performed SNP analysis of 460 6-BA-responsive DMRs via re-sequencing using a natural population of P. tomentosa, and we identified 206 SNPs that were significantly associated with growth and wood properties. Association analysis indicated that 53% of loci with allele-specific expression had primarily dominant effects on poplar traits. Our comprehensive analyses of P. tomentosa DNA methylation and the regulation of allele-specific gene expression suggest that DNA methylation is an important regulator of imbalanced expression between allelic loci.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS2011

Sensitivity of Terrestrial Water and Energy Budgets to CO2-Physiological Forcing: An Investigation Using an Offline Land Model

Increasing concentrations of atmospheric carbon dioxide (CO2) influence climate by suppressing canopy transpiration in addition to its well-known greenhouse gas effect. The decrease in plant transpiration is due to changes in plant physiology (reduced opening of plant stomata). Here, we quantify such changes in water flux for various levels of CO2 concentrations using the National Center for Atmospheric Research s (NCAR) Community Land Model. We find that photosynthesis saturates after 800 ppmv (parts per million, by volume) in this model. However, unlike photosynthesis, canopy transpiration continues to decline at about 5.1% per 100 ppmv increase in CO2 levels. We also find that the associated reduction in latent heat flux is primarily compensated by increased sensible heat flux. The continued decline in canopy transpiration and subsequent increase in sensible heat flux at elevated CO2 levels implies that incremental warming associated with the physiological effect of CO2 will not abate at higher CO2 concentrations, indicating important consequences for the global water and carbon cycles from anthropogenic CO2 emissions. Keywords: CO2-physiological effect, CO2-fertilization, canopy transpiration, water cycle, runoff, climate change 1.

Gopalakrishnan, Ranjith↗
DOE OSTI2020

Root traits as drivers of plant and ecosystem functioning: current understanding, pitfalls and future research needs

The effects of plants on the biosphere, atmosphere and geosphere are key determinants of terrestrial ecosystem functioning. However, despite substantial progress made regarding plant belowground components, we are still only beginning to explore the complex relationships between root traits and functions. Drawing on the literature in plant physiology, ecophysiology, ecology, agronomy and soil science, we reviewed 24 aspects of plant and ecosystem functioning and their relationships with a number of root system traits, including aspects of architecture, physiology, morphology, anatomy, chemistry, biomechanics and biotic interactions. Based on this assessment, we critically evaluated the current strengths and gaps in our knowledge, and identify future research challenges in the field of root ecology. Most importantly, we found that belowground traits with the broadest importance in plant and ecosystem functioning are not those most commonly measured. Also, the estimation of trait relative importance for functioning requires us to consider a more comprehensive range of functionally relevant traits from a diverse range of species, across environments and over time series. Finally, we also advocate that establishing causal hierarchical links among root traits will provide a hypothesis-based framework to identify the most parsimonious sets of traits with the strongest links on functions, and to link genotypes to plant and ecosystem functioning.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Coupled Gas-Exchange Model for C4 Leaves Comparing Stomatal Conductance Models

Plant simulation models are abstractions of plant physiological processes that are useful for investigating the responses of plants to changes in the environment. Because photosynthesis and transpiration are fundamental processes that drive plant growth and water relations, a leaf gas-exchange model that couples their interdependent relationship through stomatal control is a prerequisite for explanatory plant simulation models. Here, we present a coupled gas-exchange model for C4 leaves incorporating two widely used stomatal conductance submodels: Ball–Berry and Medlyn models. The output variables of the model includes steady-state values of CO2 assimilation rate, transpiration rate, stomatal conductance, leaf temperature, internal CO2 concentrations, and other leaf gas-exchange attributes in response to light, temperature, CO2, humidity, leaf nitrogen, and leaf water status. We test the model behavior and sensitivity, and discuss its applications and limitations. The model was implemented in Julia programming language using a novel modeling framework. Our testing and analyses indicate that the model behavior is reasonably sensitive and reliable in a wide range of environmental conditions. The behavior of the two model variants differing in stomatal conductance submodels deviated substantially from each other in low humidity conditions. The model was capable of replicating the behavior of transgenic C4 leaves under moderate temperatures as found in the literature. The coupled model, however, underestimated stomatal conductance in very high temperatures. This is likely an inherent limitation of the coupling approaches using Ball–Berry type models in which photosynthesis and stomatal conductance are recursively linked as an input of the other.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS2003

Journal of Gravitational Physiology

The topics addressed in this issue include: 1) Biosatellites: Past, Present, and Future; 2) Plant Physiology; 3) Neural/Sensory; 4) Musculoskeletal; 5) Endocrinology/Immunology; 6) Development; 7) Cardiovascular; 8) Countermeasures/Applied; 9) Technical.

Fuller, Charles↗