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At least 55 records · Page 3

Gas Exchange, Transpiration and Yield of Sweetpotato Grown in a Controlled Environment

Sweetpotato was grown to harvest maturity within NASA Johnson Space Center's Variable Pressure Growth Chamber (VPGC) to characterize crop performance for potential use in advanced life support systems as a contributor to food production, air revitalization and resource recovery. Stem cuttings of breeding clone "TU-82-155" were grown hydroponically at a density of 17 plants m(sup -2) using a modified pressure-plate growing system (Patent No. 4860-490, Tuskegee University). Lighting was provided by HPS lamps at a photoperiod of 12h light: 12h dark. The photosynthetic photon flux was maintained at 500, 750 and 1000 micro mol m(sup -2) s(sub -1) during days 1-15, 16-28, 29-119, respectively. Canopy temperatures were maintained at 28 C: light: 22 C:dark. During the light period, relative humidity and carbon dioxide were maintained at 70% and 1200 micro liters l(sup -1), respectively. Nutrient solution was manually adjusted 2 to 4 times per week by addition of 10X concentrated modified half-strength Hoagland nutrient salts and NaOH to return the electrical conductivity and pH to 1.2 mS cm(sup -1) and 6.0, respectively. At 17 weeks (119 days) from transplanting, a total of 56.5 kilograms fresh mass of storage roots (84.1% moisture) were harvested from the 11.2 m(sup 2) chamber, resulting in a yield 5.0 kilograms m(sup -2). Harvest index, based on fresh mass, was 38.6%. Rates of net photosynthesis, dark respiration, transpiration, and ethylene production will be reported.

Barta, Daniel J.↗

Evaluation of the 7-km GEOS-5 Nature Run

This report documents an evaluation by the Global Modeling and Assimilation Office (GMAO) of a two-year 7-km-resolution non-hydrostatic global mesoscale simulation produced with the Goddard Earth Observing System (GEOS-5) atmospheric general circulation model. The simulation was produced as a Nature Run for conducting observing system simulation experiments (OSSEs). Generation of the GEOS-5 Nature Run (G5NR) was motivated in part by the desire of the OSSE community for an improved high-resolution sequel to an existing Nature Run produced by the European Centre for Medium-Range Weather Forecasts (ECMWF), which has served the community for several years. The intended use of the G5NR in this context is for generating simulated observations to test proposed observing system designs regarding new instruments and their deployments. Because NASA's interest in OSSEs extends beyond traditional weather forecasting applications, the G5NR includes, in addition to standard meteorological components, a suite of aerosol types and several trace gas concentrations, with emissions downscaled to 10 km using ancillary information such as power plant location, population density and night-light information. The evaluation exercise described here involved more than twenty-five GMAO scientists investigating various aspects of the G5NR performance, including time mean temperature and wind fields, energy spectra, precipitation and the hydrological cycle, the representation of waves, tropical cyclones and midlatitude storms, land and ocean surface characteristics, the representation and forcing effects of clouds and radiation, dynamics of the stratosphere and mesosphere, and the representation of aerosols and trace gases. Comparisons are made with observational data sets when possible, as well as with reanalyses and other long model simulations. The evaluation is broad in scope, as it is meant to assess the overall realism of basic aspects of the G5NR deemed relevant to the conduct of OSSEs. However, because of the relatively short record and other practical considerations, these comparisons cannot provide a definitive, statistically sound assessment of all model deficiencies, or guarantee the G5NR's suitability for all OSSE applications. Differences between the observed and simulated behavior also must be judged in the context of basic internal atmospheric variability which can introduce variations that are not necessarily controlled by the prescribed sea surface temperatures used in generating the G5NR. The results show that the G5NR performs well as measured by the majority of metrics applied in this evaluation. Particular benefits derived from the 7-km resolution of G5NR include realistic representations of extreme weather events in both the tropics and extratropics including tropical cyclones, Nor'easters and mesoscale convective complexes; improved representation of the diurnal cycle of precipitation over land; well-resolved surface-atmosphere interactions such as katabatic wind flows over Antarctica and Greenland; and resolution of orographically generated gravity waves that propagate into the upper atmosphere and influence the large scale circulation. Obvious deficiencies in the G5NR include a "splitting" of the inter-tropical convergence zone, which leads to a weaker-than-observed Hadley circulation and related deficiencies in the depiction of stationary wave patterns. Also, while the G5NR captures global cloud features and radiative effects well in general, close comparison with observations reveals higher-than-observed cloud brightness, likely due to an overabundance of cloud condensate; less distinct cloud minima in subtropical subsidence zones, consistent with a weak Hadley circualtion; and too few near-coastal marine stratocumulus clouds.

GEOS-5↗

The variable density aircraft concept

In the variable density aircraft concept the aircraft's density is varied by varying its volume. This is accomplished by combining a variable volume hull, which is called the dynapod, with intrinsic means for the controlled variation of a mass of working fluid or substance within the aircraft. The dynapod is a hinged structure and follows the volumetric variations of the working fluid. The result is a variable density hull, which with the attachment of power plants, etc., becomes a variable density aircraft.

Davenport, A. C.↗

Dynamics of distribution and density of phreatophytes and other arid-land plant communities

The author has identified the following significant results. Six ERTS-1 images of the Tucson area, Arizona were analyzed to detect seasonal flushes of plant growth. Paired MSS-6 and MSS-5 bulk images were analyzed, using a ratioing technique, on the Electronic Satellite Image Analysis Console at Stanford Research Institute. Because of unique phenology, desert areas, covered only briefly by dense growths of ephemeral plants, are readily discerned. Grassland, evergreen forest, and riparian communities are also uniquely defined by their phenologies. Relatively sterile areas with little or no plant growth are easily discerned as are areas with varying degrees of plant productivity. The ratioing procedure detects plant coverage in excess of a threshold lying between 25% and 50%. The method is flexible and other coverage thresholds can be used.

Turner, R. M.↗

Using Remote Sensing to Assess of Growth and Distribution for Floating Invasive Plants and Growth Response Times to Altered Environments

Management of aquatic weeds in complex watersheds and river systems present many challenges to assessment, planning and implementation of management practices for aquatic invasive plants. The Delta Region Areawide Aquatic Weed Project (DRAAWP), a USDA (U. S. Dept. of Agriculture)-sponsored area-wide project, is working to enhance planning, decision-making and operational efficiency of invasive plant management in the California Sacramento-San Joaquin Delta. Satellite and airborne remote sensing are used to map area of plant coverage and estimate biomass density to aid operations and assess management impacts on plant communities. Modeling at local and watershed scales using the SWAT (Soil Water Assessment Tool) modeling tool provides insight into land-use effects on water quality. Environmental variability in the Delta occurs across a range of time scales from long-term climate and seasonal trends to short-term water flow mediated variations. Controlled environment growth studies have been conducted to quantify the growth response of invasive aquatic plants to water quality and other environmental factors. Response time for invasive species response are examined at time scales of weeks, day, and hours using a combination of study duration and growth assessment techniques to assess water quality, temperature (air and water), and light effects. These provide response parameters for plant growth models in response to environmental variation and interact with management and economic models associated with aquatic weed management. Plant growth models are to be informed by remote sensing and applied spatially across the Delta to balance location and type of aquatic plant, growth response to altered environments and phenology. Initial utilization of remote sensing tools developed for mapping of aquatic invasive plants improved operational efficiency in management practices. These assessment methods provide a comprehensive and quantitative view of aquatic invasive plant communities in the California Delta.

Floating↗

Tip-growing cells of the moss Ceratodon purpureus Are gravitropic in high-density media

Gravity sensing in plants and algae is hypothesized to rely upon either the mass of the entire cell or that of sedimenting organelles (statoliths). Protonemata of the moss Ceratodon purpureus show upward gravitropism and contain amyloplasts that sediment. If moss sensing were whole-cell based, then media denser than the cell should prevent gravitropism or reverse its direction. Cells that were inverted or reoriented to the horizontal displayed distinct negative gravitropism in solutions of iodixanol with densities of 1.052 to 1.320 as well as in bovine serum albumin solutions with densities of 1.037 to 1.184 g cm(-3). Studies using tagged molecules of different sizes and calculations of diffusion times suggest that both types of media penetrate through the apical cell wall. Estimates of the density of the apical cell range from 1.004 to 1.085. Because protonemata grow upward when the cells have a density that is lower than the surrounding medium, gravitropic sensing probably utilizes an intracellular mass in moss protonemata. These data provide additional support for the idea that sedimenting amyloplasts function as statoliths in gravitropism.

NASA Discipline Plant Biology↗

Transcription profiling of the early gravitropic response in Arabidopsis using high-density oligonucleotide probe microarrays

Studies of plant tropisms, the directed growth toward or away from external stimuli such as light and gravity, began more than a century ago. Yet biochemical, physiological, and especially molecular mechanisms of plant tropic responses remain for the most part unclear. We examined expression of 8,300 genes during early stages of the gravitropic response using high-density oligonucleotide probe microarrays. Approximately 1.7% of the genes represented on the array exhibited significant expression changes within the first 30 min of gravity stimulation. Among gravity-induced genes were a number of genes previously implicated to be involved in gravitropism. However, a much larger number of the identified genes have not been previously associated with gravitropism. Because reorientation of plants may also expose plants to mechanical perturbations, we also compared the effects of a gentle mechanical perturbation on mRNA levels during the gravity response. It was found that approximately 39% of apparently gravity-regulated genes were also regulated by the mechanical perturbation caused by plant reorientation. Our study revealed the induction of complex gene expression patterns as a consequence of gravitropic reorientation and points to an interplay between the gravitropic and mechanical responses and to the extreme sensitivity of plants to even very gentle mechanical perturbations.

NASA Discipline Plant Biology↗

The Effect of Plant Cultivar, Growth Media, Harvest Method and Post Harvest Treatment on the Microbiology of Edible Crops

Systems for the growth of crops in closed environments are being developed and tested for potential use in space applications to provide a source of fresh food. Plant growth conditions, growth media composition and harvest methods can have an effect on the microbial population of the plant, and therefore should be considered along with the optimization of plant growth and harvest yields to ensure a safe and palatable food crop. This work examines the effect of plant cultivar, growth media, and harvest method on plant microbial populations. Twelve varieties of leafy greens and herbs were grown on a mixture of Fafard #2 and Arcillite in the pillow root containment system currently being considered for the VEGGIE plant growth unit developed by Orbitec. In addition, ,Sierra and Outredgeous lettuce varieties were grown in three different mixtures (Fafard #2, Ardllite, and Perlite/Vermiculite). The plants were analyzed for microbial density. Two harvest methods, "cut and come again" (CACA) and terminal harvest were also compared. In one set ofexpe'riments red leaf lettuce and mizuna were grown in pots in a Biomass Production System for education. Plants were harvested every two weeks by either method. Another set of experiments was performed using the rooting pillows to grow 5 varieties of leafy greens and cut harvesting at different intervals. Radishes were harvested and replanted at two-week intervals. Results indicate up to a 3 IOglO difference in microbial counts between some varieties of plants. Rooting medium resulted in an approximately 2 IOglO lower count in the lettuce grown in arscillite then those grown in the other mixtures. Harvest method and frequency had less impact on microbial counts only showing a significant increase in one variety of plant. Post harvest methods to decrease the bacterial counts on edible crops were investigated in these and other experiments. The effectiveness of PRO-SAN and UV-C radiation is compared.

Hummerick, Mary P.↗

Discrete anisotropic radiative transfer modelling of solar-induced chlorophyll fluorescence: Structural impacts in geometrically explicit vegetation canopies

Solar-induced fluorescence (SIF) is a subtle but informative optical signal of vegetation photosynthesis. Remotely sensed SIF integrates environmental, physiological and structural changes that alter photosynthesis at leaf, plant and canopy scales. Radiative transfer models are ideally suited to investigate the complex sources of variability in the SIF signal to guide the interpretation of SIF retrievals from airborne and space-borne platforms. Here, we coupled the Fluspect-Cx model of leaf optical properties and chlorophyll-a fluorescence with the Discrete Anisotropic Radiative Transfer (DART) model to upscale SIF from individual leaves to three-dimensional (3D) structurally explicit canopies. For one-dimensional homogeneous (turbid-like) canopies, DART-SIF was nearly identical to SIF simulated in two existing models, SCOPE and mSCOPE (RMSE <0.221 W./sq.m.μm.sr). DART simulations in geometrically explicit 3D canopies offered four important insights regarding the influence of vegetation structure on the multi-angular top-of-canopy SIF signal. First, changes in the 3D canopy architecture of maize crops, represented by leaf density (leaf area index), and plant clumping (canopy closure) had a larger impact on SIF than the modelled photosynthetic efficiency distinction between sun-adapted and shade-adapted foliage. Second, clumping of leaves at the crop and stand levels was identified as one of the key driving factors of multi-angular anisotropy of red and far-red SIF (686 and 740 nm) for both maize and eucalyptus canopies. Third, non-photosynthetic woody material had a significant impact on top-of-canopy SIF in modelled 3D forest stands. Wood shadowing decreased the photosynthetically active radiation absorbed by green leaves, and consequently the SIF emissions, by 10% in sparse and 17% in dense eucalyptus stands. The wood obstruction (blocking) effect, quantified as a relative difference of SIF escape probabilities from canopies with and without wood in the nadir viewing direction, decreased far-red SIF by 4–6% but it had a smaller and sometimes positive influence (by less than 2%) on red SIF. Fourth, DART 3D radiative budget profiles revealed that the majority of the SIF signal from a dense eucalyptus stand originated from the top 25% of the simulated canopy. Interestingly, the introduction of bark-covered woody elements did not alter the simulated balance and omnidirectional escape factor of red SIF in this upper canopy part but did raise significantly both of them in case of far-red SIF. These results demonstrate the importance of 3D radiative transfer and radiative budget simulations for investigating SIF interactions in structurally complex plant canopies and for a better understanding of spatiotemporal and multi-angular remote sensing SIF observations.

Zbyněk Malenovský↗

Nitrogen balance for wheat canopies (Triticum aestivum cv. Veery 10) grown under elevated and ambient CO2 concentrations

We examined the hypothesis that elevated CO2 concentration would increase NO3- absorption and assimilation using intact wheat canopies (Triticum aestivum cv. Veery 10). Nitrate consumption, the sum of plant absorption and nitrogen loss, was continuously monitored for 23 d following germination under two CO2 concentrations (360 and 1000 micromol mol-1 CO2) and two root zone NO3- concentrations (100 and 1000 mmol m3 NO3-). The plants were grown at high density (1780 m-2) in a 28 m3 controlled environment chamber using solution culture techniques. Wheat responded to 1000 micromol mol-1 CO2 by increasing carbon allocation to root biomass production. Elevated CO2 also increased root zone NO3- consumption, but most of this increase did not result in higher biomass nitrogen. Rather, nitrogen loss accounted for the greatest part of the difference in NO3- consumption between the elevated and ambient [CO2] treatments. The total amount of NO3(-)-N absorbed by roots or the amount of NO3(-)-N assimilated per unit area did not significantly differ between elevated and ambient [CO2] treatments. Instead, specific leaf organic nitrogen content declined, and NO3- accumulated in canopies growing under 1000 micromol mol-1 CO2. Our results indicated that 1000 micromol mol-1 CO2 diminished NO3- assimilation. If NO3- assimilation were impaired by high [CO2], then this offers an explanation for why organic nitrogen contents are often observed to decline in elevated [CO2] environments.

Non-NASA Center↗

An improvement in land cover classification achieved by merging microwave data with Landsat multispectral scanner data

The improvement in land cover classification achieved by merging microwave data with Landsat MSS data is examined. To produce a merged data set for analysis and comparison, a registration procedure by which a set of Seasat SAR digital data was merged with the MSS data is described. The Landsat MSS data and the merged Landsat/Seasat data sets were processed using conventional multichannel spectral pattern recognition techniques. An analysis of the classified data sets indicates that while Landsat data delineate different forest types (i.e., deciduous/coniferous) and allow some species separation, SAR data provide additional information related to plant canopy configuration and vegetation density as associated with varying water regimes, and therefore allow for further subdivision in the classification of forested wetlands of the coastal region of the southern United States.

Wu, S. T.↗

SO2 Plumes Observation with LMOL: Theory, Modeling, and Validation

LMOL, the NASA Langley Mobile Ozone Lidar, is locatednear NASA’s LaRC steam plant when not deployed in campaigns. Theplant produces steam through the incineration of local trash, and itsexhaust plume occasionally contains SO2.SO2is a known, regulated, pollutant that affects O3observations in theUV, as is the case with LMOL. In this work, we show how we modifiedLMOL to detect the plant SO2plumes and compute its density when theO3background is stable; we observed densities compatible with what isexpected from the typical plume exhaust. The selection of laser lines sothat an O3variation would not get confused with a SO2detection isexplained in details, and a model capable of simulating the Lidar signalwith (notably) O3and SO2absorption for the validation of the retrievalresolution and uncertainty is presented. Finally, the comparison betweenthe modeled and observed performances of the system is shown: themaximum altitude, resolution, and error in the modeled signal and theobserved signals are the same within reasonable margins.This work demonstrates that LMOL is fully capable of working withSO2. The next step being the addition of an additional laser channel,which is simplified by the tunable aspect of the LMOL laser, to addressO3and SO2simultaneously, allowing the assessment of SO2when O3has variations.

Guillaume Gronoff↗

Soluble carbohydrate allocation to roots, photosynthetic rate of leaves, and nitrate assimilation as affected by nitrogen stress and irradiance

Upon resupply of exogenous nitrogen to nitrogen-stressed plants, uptake rate of nitrogen is enhanced relative to nonstressed plants. Absorption of nitrogen presumably is dependent on availability of carbohydrates in the roots. A buildup in soluble carbohydrates thus should occur in roots of nitrogen-stressed plants, and upon resupply of exogenous nitrogen the increased uptake rate should be accompanied by a rapid decline in carbohydrates to prestress levels. To evaluate this relationship, three sets of tobacco plants growing in a complete hydroponic solution containing 1.0 mM NO3- were either continued in the complete solution for 21 d, transferred to a minus-nitrogen solution for 21 d, or transferred to a minus-nitrogen solution for 8-9 d and then returned to the 1.0 mM NO3- solution. These nitrogen treatments were imposed upon plants growing at photosynthetic photon flux densities of 700 and 350 micromoles m-2 s-1. Soluble carbohydrate levels in roots increased during onset of nitrogen stress to levels that were fourfold greater than in roots of non-stressed plants. Following resupply of external nitrogen, a rapid resumption of nitrogen uptake was accompanied by a decline in soluble carbohydrates in roots to levels characteristic of nonstressed plants. This pattern of soluble carbohydrate levels in roots during onset of and recovery from nitrogen stress occurred at both irradiance levels. The response of net photosynthetic rate to nitrogen stress could be expressed as a nonlinear function of concentration of reduced nitrogen in leaves. The net photosynthetic rate at a given concentration of reduced nitrogen, however, averaged 10% less at the lower than at the higher irradiance. The decline in net photosynthetic rate per unit of reduced nitrogen in leaves at the lower irradiance was accompanied by an increase in the nitrate fraction of total nitrogen in leaves from 20% at the higher irradiance to 38% at the lower irradiance.

NASA Discipline Number 61-10↗

High energy efficiency and high power density proton exchange membrane fuel cells: Electrode kinetics and mass transport

The development of proton exchange membrane (PEM) fuel cell power plants with high energy efficiencies and high power densities is gaining momentum because of the vital need of such high levels of performance for extraterrestrial (space, underwater) and terrestrial (power source for electric vehicles) applications. Since 1987, considerable progress has been made in achieving energy efficiencies of about 60 percent at a current density of 200 mA/sq cm and high power densities (greater than 1 W/sq cm) in PEM fuel cells with high (4 mg/sq cm) or low (0.4 mg/sq cm) platinum loadings in electrodes. The following areas are discussed: (1) methods to obtain these high levels of performance with low Pt loading electrodes - by proton conductor impregnation into electrodes, localization of Pt near front surface; (2) a novel microelectrode technique which yields electrode kinetic parameters for oxygen reduction and mass transport parameters; (3) demonstration of lack of water transport from anode to cathode; (4) modeling analysis of PEM fuel cell for comparison with experimental results and predicting further improvements in performance; and (5) recommendations of needed research and development for achieving the above goals.

Supramaniam Srinivasan↗