Search NASA⌕ Search

SEARCH · Search NASA

Results for “precursors engineering”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 55 records · Page 3
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Autonomous stabilization with programmable stabilized state

Reservoir engineering is a powerful technique to autonomously stabilize a quantum state. Traditional schemes involving multi-body states typically function for discrete entangled states. In this work, we enhance the stabilization capability to a continuous manifold of states with programmable stabilized state selection using multiple continuous tuning parameters. We experimentally achieve 84.6% and 82.5% stabilization fidelity for the odd and even-parity Bell states as two special points in the manifold. We also perform fast dissipative switching between these opposite parity states within 1.8 μs and 0.9 μs by sequentially applying different stabilization drives. Our result is a precursor for new reservoir engineering-based error correction schemes.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
NASA NTRS2004

Accident Precursor Analysis and Management: Reducing Technological Risk Through Diligence

Almost every year there is at least one technological disaster that highlights the challenge of managing technological risk. On February 1, 2003, the space shuttle Columbia and her crew were lost during reentry into the atmosphere. In the summer of 2003, there was a blackout that left millions of people in the northeast United States without electricity. Forensic analyses, congressional hearings, investigations by scientific boards and panels, and journalistic and academic research have yielded a wealth of information about the events that led up to each disaster, and questions have arisen. Why were the events that led to the accident not recognized as harbingers? Why were risk-reducing steps not taken? This line of questioning is based on the assumption that signals before an accident can and should be recognized. To examine the validity of this assumption, the National Academy of Engineering (NAE) undertook the Accident Precursors Project in February 2003. The project was overseen by a committee of experts from the safety and risk-sciences communities. Rather than examining a single accident or incident, the committee decided to investigate how different organizations anticipate and assess the likelihood of accidents from accident precursors. The project culminated in a workshop held in Washington, D.C., in July 2003. This report includes the papers presented at the workshop, as well as findings and recommendations based on the workshop results and committee discussions. The papers describe precursor strategies in aviation, the chemical industry, health care, nuclear power and security operations. In addition to current practices, they also address some areas for future research.

Phimister, James R.↗
DOE OSTI2024

Verazine biosynthesis from simple sugars in engineered Saccharomyces cerevisiae

Steroidal alkaloids are FDA-approved drugs (e.g., Zytiga) and promising drug candidates/leads (e.g., cyclopamine); yet many of the ≥697 known steroidal alkaloid natural products remain underutilized as drugs because it can be challenging to scale their biosynthesis in their producing organisms. Cyclopamine is a steroidal alkaloid produced by corn lily (Veratrum spp.) plants, and it is an inhibitor of the Hedgehog (Hh) signaling pathway. Therefore, cyclopamine is an important drug candidate/lead to treat human diseases that are associated with dysregulated Hh signaling, such as basal cell carcinoma and acute myeloid leukemia. Cyclopamine and its semi-synthetic derivatives have been studied in (pre)clinical trials as Hh inhibitor-based drugs. However, challenges in scaling the production of cyclopamine have slowed efforts to improve its efficacy and safety profile through (bio)synthetic derivatization, often limiting drug development to synthetic analogs of cyclopamine such as the FDA-approved drugs Odomzo, Daurismo, and Erivedge. If a platform for the scalable and sustainable production of cyclopamine were established, then its (bio)synthetic derivatization, clinical development, and, ultimately, widespread distribution could be accelerated. Ongoing efforts to achieve this goal include the biosynthesis of cyclopamine in Veratrum plant cell culture and the semi-/total chemical synthesis of cyclopamine. Herein, this work advances efforts towards a promising future approach: the biosynthesis of cyclopamine in engineered microorganisms. We completed the heterologous microbial production of verazine (biosynthetic precursor to cyclopamine) from simple sugars (i.e., glucose and galactose) in engineered Saccharomyces cerevisiae (S. cerevisiae) through the inducible upregulation of the native yeast mevalonate and lanosterol biosynthetic pathways, diversion of biosynthetic flux from ergosterol (i.e., native sterol in S. cerevisiae) to cholesterol (i.e., biosynthetic precursor to verazine), and expression of a refactored five-step verazine biosynthetic pathway. The engineered S. cerevisiae strain that produced verazine contains eight heterologous enzymes sourced from seven different species. Importantly, S. cerevisiae-produced verazine was indistinguishable via liquid chromatography-mass spectrometry from both a commercial standard (Veratrum spp. plant-produced) and Nicotiana benthamiana-produced verazine. To the best of our knowledge, this is the first report describing the heterologous production of a steroidal alkaloid in an engineered yeast. Verazine production was ultimately increased through design-build-test-learn cycles to a final titer of 83 ± 3 μg/L (4.1 ± 0.1 μg/g DCW). Finally, this research lays the groundwork for future microbial biosynthesis of cyclopamine, (bio)synthetic derivatives of cyclopamine, and other steroidal alkaloid natural products.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Conjugation-based genome engineering enables rapid prototyping and bioproduction in non-model bacteria

Abstract Non-model bacteria offer unique metabolic capabilities for sustainable bioproduction, yet their limited genetic accessibility hinders systematic strain development. Here we present conjugation-based serine recombinase-assisted genome engineering (cSAGE), a broad-host-range platform that enables predictable, iterative genomic integration in transformation-resistant bacteria. cSAGE combines conjugative DNA delivery, standardized low-copy vectors, orthogonal recombinases, and modular genetic parts to support rapid pathway assembly and cross-host benchmarking. Using purple nonsulfur bacteria as a testbed, we integrate promoter engineering, multi-payload genome modification, and genome-scale metabolic modeling to empirically evaluate host-dependent pathway performance. Applying this workflow, we identify strain-specific differences in photosynthetic conversion of lignin-derived p -coumarate to the thermoplastic precursor p -vinylphenol. By enabling genome engineering and functional comparison across diverse bacteria using a single plasmid system, cSAGE provides a general framework for non-model strain prototyping and biotransformation discovery.

Guzman, Michael S. [Department of Chemical Enginee↗
DOE OSTI2021

Lightweight Materials - Carbon Fiber and Polymer Composites, Integrated Computational Materials Engineering (ICME) Predictive Tools Development for Low-Cost Carbon Fiber for Lightweight Vehicles (University of Virginia) (Final Report)

This project aimed to develop low-cost alternative precursors and processing techniques through the implementation of an integrated computational materials engineering (ICME) framework to evaluate precursor conversion kinetics. Once established, this framework was then used to down-select precursors for laboratory- and pilot-scale production to validate resulting carbon fiber (CF) material properties and process cost-savings. The two most promising approaches to reduce the cost of CFs were to (1) use new low-cost precursors and (2) reduce the energy requirements of CF processing. These approaches were investigated over the course of three phases of work, spanning 3.5 years. This report describes the overall project objectives, approach, and the key accomplishments in each phase.

36 MATERIALS SCIENCE↗
NASA NTRS2000

Propulsion Options For Interstellar Exploration

NASA is considering missions to explore near-interstellar space (40 - 250 Astronomical Units) early in the next decade as the first step toward a vigorous interstellar exploration program. A key enabling technology for such an ambitious science and exploration effort is a propulsion system capable of providing fast trip times, yet which has low enough mass to allow for the use of inexpensive launch vehicles. Advanced propulsion technologies that might support the First interstellar precursor mission by the end of the first decade of the new millennium include solar sails and nuclear electric propulsion. Solar sails and electric propulsion are two technology areas that may hold promise for the next generation of interstellar precursor missions as well - perhaps a thousand astronomical units traveled in a professional lifetime. Future missions to far beyond the Heliosphere will require the development of propulsion technologies that are only at the conceptual stage today. For years, the scientific community has been interested in solar sail and electric propulsion technologies to support robotic exploration of the solar system. Progress in thin-film materials fabrication and handling, and advancement in technologies that may enable the deployment of large sails in space are only now maturing to the point where ambitious interstellar precursor missions using sails can be considered. Xenon ion propulsion is now being demonstrated for planetary exploration by the Deep Space 1 mission. The primary issues for the adaptation of electric propulsion to interstellar precursor applications include the development of low specific mass nuclear power systems, engine lifetime, and high power operation. Recent studies of interstellar precursor mission scenarios that use these propulsion systems will be described, and the range of application of each technology will be explored.

Johnson, Les↗
NASA NTRS1999

Trace Chemistry

The goals of the trace chemistry group were to identify the processes relevant to aerosol and aerosol precursor formation occurring within aircraft gas turbine engines; that is, within the combustor, turbine, and nozzle. The topics of discussion focused on whether the chemistry of aerosol formation is homogeneous or heterogeneous; what species are important for aerosol and aerosol precursor formation; what modeling/theoretical activities to pursue; what experiments to carry out that both support modeling activities and elucidate fundamental processes; and the role of particulates in aerosol and aerosol precursor formation. The consensus of the group was that attention should be focused on SO2, SO3, and aerosols. Of immediate concern is the measurement of the concentration of the species SO3, SO2, H2SO4 OH, HO2, H2O2, O, NO, NO2, HONO, HNO3, CO, and CO2 and particulates in various engines, both those currently in use and those in development. The recommendation was that concentration measurements should be made at both the combustor exit and the engine exit. At each location the above species were classified into one of four categories of decreasing importance, Priority I through IV, as follows: Combustor exit: Priority I species - SO3:SO2 ratio, SO3, SO2, and particulates; Priority II species: OH and O; Priority III species - NO and NO2; and Priority IV species - CO and CO2. For the Engine exit: Priority I species - SO3:SO2 ratio, SO3, SO2,H2SO4, and particulates; Priority II species: OH,HO2, H2O2, and O; Priority III species - NO, NO2, HONO, and HNO3; and Priority IV species - CO and CO2. Table I summarizes the anticipated concentration range of each of these species. For particulate matter, the quantities of interest are the number density, size distribution, and composition. In order to provide data for validating multidimensional reacting flow models, it would be desirable to make 2-D, time-resolved measurements of the concentrations of the above species and, in addition, of the pressure, temperature, and velocity. A near term goal of the experimental program should be to confirm the nonlinear effects of sulfur speciation, and if present, to provide an explanation for them. It is also desirable to examine if the particulate matter retains any sulfur. The recommendation is to examine the effects on SOx production of variations in fuel-bound sulfur and aromatic content (which may affect the amount of particulates formed). These experiments should help us to understand if there is a coupling between particulate formation and SO, concentration. Similarly, any coupling with NOx can be examined either by introducing NOx into the combustion air or by using fuel-bound nitrogen. Also of immediate urgency is the need to establish and validate a detailed mechanism for sulfur oxidation/aerosol formation, whose chemistry is concluded to be homogeneous, because there is not enough surface area for heterogeneous effects. It is envisaged that this work will involve both experimental and theoretical programs. The experimental work will require, in addition to the measurements described above, fundamental studies in devices such as flow reactors and shock tubes. Complementing this effort should be modeling and theoretical activities. One impediment to the successful modeling of sulfur oxidation is the lack of reliable data for thermodynamic and transport properties for several species, such as aqueous nitric acid, sulfur oxides, and sulfuric acid. Quantum mechanical calculations are recommended as a convenient means of deriving values for these properties. Such calculations would also help establish rate constants for several important reactions for which experimental measurements are inherently fraught with uncertainty. Efforts to implement sufficiently detailed chemistry into computational fluid dynamic codes should be continued. Zero- and one-dimensional flow models are also useful vehicles for elucidating the minimal set of species and reactions that must be included in two- and three-dimensional modeling studies.

Radhakrishnan, Krishnan↗
NASA NTRS2009

NASA Manned Launch Vehicle Lightning Protection Development

Historically, the National Aeronautics and Space Administration (NASA) relied heavily on lightning avoidance to protect launch vehicles and crew from lightning effects. As NASA transitions from the Space Shuttle to the new Constellation family of launch vehicles and spacecraft, NASA engineers are imposing design and construction standards on the spacecraft and launch vehicles to withstand both the direct and indirect effects of lightning. A review of current Space Shuttle lightning constraints and protection methodology will be presented, as well as a historical review of Space Shuttle lightning requirements and design. The Space Shuttle lightning requirements document, NSTS 07636, Lightning Protection, Test and Analysis Requirements, (originally published as document number JSC 07636, Lightning Protection Criteria Document) was developed in response to the Apollo 12 lightning event and other experiences with NASA and the Department of Defense launch vehicles. This document defined the lightning environment, vehicle protection requirements, and design guidelines for meeting the requirements. The criteria developed in JSC 07636 were a precursor to the Society of Automotive Engineers (SAE) lightning standards. These SAE standards, along with Radio Technical Commission for Aeronautics (RTCA) DO-160, Environmental Conditions and Test Procedures for Airborne Equipment, are the basis for the current Constellation lightning design requirements. The development and derivation of these requirements will be presented. As budget and schedule constraints hampered lightning protection design and verification efforts, the Space Shuttle elements waived the design requirements and relied on lightning avoidance in the form of launch commit criteria (LCC) constraints and a catenary wire system for lightning protection at the launch pads. A better understanding of the lightning environment has highlighted the vulnerability of the protection schemes and associated risk to the vehicle, which has resulted in lost launch opportunities and increased expenditures in manpower to assess Space Shuttle vehicle health and safety after lightning events at the launch pad. Because of high-percentage launch availability and long-term on-pad requirements, LCC constraints are no longer considered feasible. The Constellation vehicles must be designed to withstand direct and indirect effects of lightning. A review of the vehicle design and potential concerns will be presented as well as the new catenary lightning protection system for the launch pad. This system is required to protect the Constellation vehicles during launch processing when vehicle lightning effects protection might be compromised by such items as umbilical connections and open access hatches.

McCollum, Matthew B.↗
DOE OSTI2023

Discovery of the Azaserine Biosynthetic Pathway Uncovers a Biological Route for α‐Diazoester Production

Abstract Azaserine is a bacterial metabolite containing a biologically unusual and synthetically enabling α‐diazoester functional group. Herein, we report the discovery of the azaserine ( aza ) biosynthetic gene cluster from Glycomyces harbinensis . Discovery of related gene clusters reveals previously unappreciated azaserine producers, and heterologous expression of the aza gene cluster confirms its role in azaserine assembly. Notably, this gene cluster encodes homologues of hydrazonoacetic acid (HYAA)‐producing enzymes, implicating HYAA in α‐diazoester biosynthesis. Isotope feeding and biochemical experiments support this hypothesis. These discoveries indicate that a 2‐electron oxidation of a hydrazonoacetyl intermediate is required for α‐diazoester formation, constituting a distinct logic for diazo biosynthesis. Uncovering this biological route for α‐diazoester synthesis now enables the production of a highly versatile carbene precursor in cells, facilitating approaches for engineering complete carbene‐mediated biosynthetic transformations in vivo.

Van Cura, Devon↗
DOE OSTI2023

Discovery of the Azaserine Biosynthetic Pathway Uncovers a Biological Route for α‐Diazoester Production

Abstract Azaserine is a bacterial metabolite containing a biologically unusual and synthetically enabling α‐diazoester functional group. Herein, we report the discovery of the azaserine ( aza ) biosynthetic gene cluster from Glycomyces harbinensis . Discovery of related gene clusters reveals previously unappreciated azaserine producers, and heterologous expression of the aza gene cluster confirms its role in azaserine assembly. Notably, this gene cluster encodes homologues of hydrazonoacetic acid (HYAA)‐producing enzymes, implicating HYAA in α‐diazoester biosynthesis. Isotope feeding and biochemical experiments support this hypothesis. These discoveries indicate that a 2‐electron oxidation of a hydrazonoacetyl intermediate is required for α‐diazoester formation, constituting a distinct logic for diazo biosynthesis. Uncovering this biological route for α‐diazoester synthesis now enables the production of a highly versatile carbene precursor in cells, facilitating approaches for engineering complete carbene‐mediated biosynthetic transformations in vivo.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2024

Surface Engineering Strategy to Synthesize Bicomponent Carbons for Rechargeable Zinc-Air Batteries

Atomic Fe-N x moieties and nanosized FeCo species anchored on carbons have each been demonstrated to be among the most effective active components for oxygen reduction and evolution reactions (ORR/OER), respectively in rechargeable zinc -air batteries (ZABs). However, incorporating both of these components in a single catalyst presents a great challenge due to the trade-off in formation between them during hightemperature preparation. Herein, we integrate them into a bicomponent carbon through a surface engineering strategy. In this process, K 3 [Fe(CN) 6 ] is engineered on the surface of a precursor mixture consisting of polyaniline-coated graphene oxide and ZIF-67. This is followed by pyrolysis to produce the bicomponent carbon catalyst of FeCo nanoparticles modified carbon polyhedron (for accelerating the OER), supported on atomically dispersed Fe -N -doped carbon nanosheet (for boosting the ORR). The catalyst exhibits a small potential gap of 0.69 V for OER/ORR. In situ Raman spectroscopy demonstrates that spinel FeCo oxides may be responsible for OER. The use of this catalyst in ZABs achieves high power densities of 225 mW cm -2 in aqueous electrolyte and 164 mW cm -2 in solid-state electrolyte. Additionally, a small and stable voltage gap of 0.712 V at 10 mA cm -2 is maintained after 1035 discharge -charge cycles demonstrating the great application potential in energy devices.

An, Jia-Xing↗
NASA NTRS1989

Rocket plume spectrometry: A system permitting engine condition monitoring, as applied to the technology test bed engine

The appearance of visible objects in the exhaust plume of space shuttle main engines (SSME) during test firings is discussed. A program was undertaken to attempt to identify anomalous material resulting from wear, normal or excessive, of internal parts, allowing time monitoring of engine condition or detection of failure precursors. Measurements were taken during test firings at Stennis Space Center and at the Santa Suzanna facility in California. The results indicated that a system having high spectral resolution, a fast time response, and a wide spectral range was required to meet all requirements, thus two special systems have been designed and built. One is the Optical Plume Anomaly Detector (OPAD). The other instrument, which is described in this report, is the superspectrometer, an optical multichannel analyzer having 8,192 channels covering the spectral band 250 to 1,000 nm.

Powers, W. T.↗
NASA NTRSDate not supplied

Development of the Microcalorimeter Detector for Athena/X-IFU

The X-ray Integral Field Unit (X-IFU) will be a microcalorimeter instrument on ESA’s Athena X-ray observatory, set to launch around 2037. Athena recently underwent a reformulation exercise resulting in a simplified X-IFU architecture, with a new baseline configuration featuring fewer pixels and readout channels. Newly developed transition edge sensor pixels that are slower and have reduced magnetic field sensitivity have helped enable the new instrument design whilst maintaining flagship quality science. In this paper, we report on the status and plan for continued development of the X-IFU microcalorimeter array. We describe details of the current pixel architecture and performance specifications. We will present the design of the first full scale microcalorimeter arrays that have the necessary flight like electrical and mechanical interfaces for X-IFU. These arrays will undergo testing in a focal-plane assembly (FPA) Development Model (DM) in Europe later in 2024 and serve as a precursor to the X-IFU Engineering Model detector.

Stephen J Smith↗
DOE OSTI2023

Effect of tension during sulfonation stabilization for UHMWPE-derived carbon fibers

Polyethylene is a promising low-cost alternative precursor material for carbon fiber production, but it has yet to show mechanical properties near or surpassing polyacrylonitrile-derived carbon fibers. The high molecular weight and order of ultra-high molecular weight polyethylene (UHMWPE) may offer a pathway to realizing this promise by enabling long-range graphitic structure formation and superior mechanical properties. The tension applied during the precursor stabilization process is crucial to maintaining the shape of the fibers during the conversion process, but no published study has yet probed the relationship between sulfonation tension and carbon fiber microstructure and mechanical properties. In this work, a logarithmic sweep of tensile stress was applied to UHMWPE fibers during the stabilization process followed by carbonization. Increasing tension significantly reduced fiber shrinkage, resulted in straighter fibers with less severe kink bands, and greatly improved the mechanical properties of the fibers. Raman spectroscopy and X-ray diffraction revealed that in all cases the carbon fibers were largely amorphous, but increasing tension resulted in increased size and alignment of the turbostratic crystallites with the fiber axis. Large voids were present in the sample fibers, so the Griffith-Irwin relation was employed to predict the potential ultimate tensile strength of the fibers with voids reduced to sizes comparable to commercially produced fibers. Furthermore, this work demonstrates the importance of tension applied during the stabilization of polyethylene fibers for carbon fiber production and establishes a framework for achieving high mechanical properties from these precursors.

36 MATERIALS SCIENCE↗
DOE OSTI2024

Complete biosynthesis of QS-21 in engineered yeast

QS-21 is a potent vaccine adjuvant and remains the only saponin-based adjuvant that has been clinically approved for use in humans. However, owing to the complex structure of QS-21, its availability is limited. Today, the supply depends on laborious extraction from the Chilean soapbark tree or on low-yielding total chemical synthesis. Here we demonstrate the complete biosynthesis of QS-21 and its precursors, as well as structural derivatives, in engineered yeast strains. The successful biosynthesis in yeast requires fine-tuning of the host’s native pathway fluxes, as well as the functional and balanced expression of 38 heterologous enzymes. The required biosynthetic pathway spans seven enzyme families—a terpene synthase, P450s, nucleotide sugar synthases, glycosyltransferases, a coenzyme A ligase, acyl transferases and polyketide synthases—from six organisms, and mimics in yeast the subcellular compartmentalization of plants from the endoplasmic reticulum membrane to the cytosol. Finally, by taking advantage of the promiscuity of certain pathway enzymes, we produced structural analogues of QS-21 using this biosynthetic platform. This microbial production scheme will allow for the future establishment of a structure–activity relationship, and will thus enable the rational design of potent vaccine adjuvants.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1992

Precursor SSF utilization: The MODE experiments

The MIT Space Engineering Research Center is the principal investigator for a series of experiments which utilize the Shuttle Middeck as an engineering dynamics laboratory. The first, which flew on STS-48 in Sep. 1991, was the Middeck O-gravity Dynamics Experiment (MODE). This experiment focused on the dynamics of a scaled deployable truss, similar to that of SSF, and contained liquids in tanks. MODE will be reflown in the fall of 1993. In mid-1994, the Middeck Active Control Experiment (MACE) will examine the issues associated with predicting and verifying the closed loop behavior of a controlled structure in zero gravity. The paper will present experiment background, planning, operational experience, results, and lessons learned from these experiments which are pertinent to SSF utilization.

Crawley, Edward F.↗
NASA NTRS1988

Plume spectrometry for liquid rocket engine health monitoring

An investigation of Space Shuttle Main Engine (SSME) testing failures identified optical events which appeared to be precursors of those failures. A program was therefore undertaken to detect plume trace phenomena characteristic of the engine and to design a monitoring system, responsive to excessive activity in the plume, capable of delivering a warning of an anomalous condition. By sensing the amount of extraneous material entrained in the plume and considering engine history, it may be possible to identify wearing of failing components in time for a safe shutdown and thus prevent a catastrophic event. To investigate the possibilities of safe shutdown and thus prevent a monitor to initiate the shutdown procedure, a large amount of plume data were taken from SSME firings using laboratory instrumentation. Those data were used to design a more specialized instrument dedicated to rocket plume diagnostics. The spectral wavelength range of the baseline data was about 220 nanometers (nm) to 15 micrometer with special attention given to visible and near UV. The data indicates that a satisfactory design will include a polychromator covering the range of 250 nM to 1000 nM, along with a continuous coverage spectrometer, each having a resolution of at least 5A degrees. The concurrent requirements for high resolution and broad coverage are normally at odds with one another in commercial instruments, therefore necessitating the development of special instrumentation. The design of a polychromator is reviewed herein, with a detailed discussion of the continuous coverage spectrometer delayed to a later forum. The program also requires the development of applications software providing detection, variable background discrimination, noise reduction, filtering, and decision making based on varying historical data.

Powers, William T.↗