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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 55 records · Page 3
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Temporal patterns of fine‐root dynamics have little influence on seasonal soil CO 2 efflux in a mixed, mesic forest

Among the contributors to soil CO 2 efflux, there remains uncertainty about the contribution of root activity to the overall soil efflux. Soil water and temperature frequently have been used to predict a large portion of the variation in soil CO 2 efflux. We hypothesized that fine-root dynamics explain most of the remaining variability in soil CO 2 efflux that cannot be explained by soil temperature and water content. We anticipated that seasonal increases in root production, mortality via decomposition, and standing crop would result in corresponding increases in soil CO 2 efflux. We tested our hypotheses by collecting and analyzing two years of minirhizotron and soil chamber CO 2 flux data from plots distributed throughout the Shale Hills Catchment of the Susquehanna-Shale Hills Critical Zone Observatory in Central Pennsylvania, USA. Here we showed that: (1) seasonal fluctuations in fine-root dynamics yielded only a very small increase in the predictability of soil CO 2 efflux; (2) fine-root mortality effects on soil CO 2 efflux were strongly tied to soil temperature; (3) fluctuations in fine-root presence or standing mass independent of temperature and moisture had little effect on soil CO 2 efflux; and (4) new fine-root length and root length mortality had limited impacts on soil CO 2 efflux rates. We conclude that, at least in temperate forests on rocky soils, characterizing fine-root dynamics may provide only limited improvement in the estimation of soil CO 2 efflux.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2023

Expression of macromolecular organic nitrogen degrading enzymes identifies potential mediators of soil organic N availability to an annual grass

Abstract Nitrogen (N) is frequently limiting to plant growth, in part because most soil N is present as polymeric organic compounds that are not readily taken up by plants. Microbial depolymerization of these large macromolecular N-substrates gradually releases available inorganic N. While many studies have researched and modeled controls on soil organic matter formation and bulk N mineralization, the ecological—spatial, temporal and phylogenetic—patterns underlying organic N degradation remain unclear. We analyzed 48 time-resolved metatranscriptomes and quantified N-depolymerization gene expression to resolve differential expression by soil habitat and time in specific taxonomic groups and gene-based guilds. We observed much higher expression of extracellular serine-type proteases than other extracellular N-degrading enzymes, with protease expression of predatory bacteria declining with time and other taxonomic patterns driven by the presence (Gammaproteobacteria) or absence (Thermoproteota) of live roots and root detritus (Deltaproteobacteria and Fungi). The primary chitinase chit1 gene was more highly expressed by eukaryotes near root detritus, suggesting predation of fungi. In some lineages, increased gene expression over time suggests increased competitiveness with rhizosphere age (Chloroflexi). Phylotypes from some genera had protease expression patterns that could benefit plant N nutrition, for example, we identified a Janthinobacterium phylotype and two Burkholderiales that depolymerize organic N near young roots and a Rhizobacter with elevated protease levels near mature roots. These taxon-resolved gene expression results provide an ecological read-out of microbial interactions and controls on N dynamics in specific soil microhabitats and could be used to target potential plant N bioaugmentation strategies.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2022

Whole plant traits of coastal dune vegetation and implications for interactions with dune dynamics

Abstract Coastal dunes are important protective features against sea level rise and coastal storms. Interactions between dune plant aboveground structures and sediment trapping that allow for dune building and maintenance are well established. More recently, studies documenting belowground biomass for promoting erosion resistance in dominant dune species have been conducted, yet a knowledge gap remains regarding species‐specific characterization of whole plants, specifically with respect to roots, rhizomes, and belowground stems. Our objective was to quantify above‐ and belowground traits of four dominant dune grasses to document the potential for species‐specific effects on dune growth, maintenance, and erosion resistance. We examined above‐ and belowground traits among four prominent dune grasses of the Atlantic and Gulf Coasts of North America: Ammophila breviligulata , Panicum amarum , Spartina patens , and Uniola paniculata . Whole plant samples of each species were collected from the foredune at the US Army Engineer Research and Development Center's Field Research Facility in Duck, North Carolina, USA, and quantified for several above‐ and belowground traits (e.g., stem height, rhizome number and length, root surface area by diameter class, root tensile strength, and mycorrhizal percent infection). Belowground factors known to impact important dune processes, such as rhizome length, mycorrhizal percent infection, and root traits, differed substantially among species. When visualized in multivariate space, all species significantly differed in suites of above‐ and belowground traits. When considering belowground only, Ammophila and Spartina were similar, despite differences in biomass allocation. Species separated along axes related to mycorrhizal association, biomass allocation, and root construction. The four co‐occurring dune grass species were dissimilar in suites of plant traits. Belowground trait differences were driven by those describing root construction, biomass allocation, and mycorrhizal infection. Dissimilarity in above‐ and belowground suites of traits may demonstrate different approaches for surviving the dune environment. Incorporating belowground traits into modeling will enhance predictions of dune response to climate change through interactions between vegetation and dune dynamics that facilitate coastal resistance and resilience.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1990

Binding of isolated plant lectin by rhizobia during episodes of reduced gravity obtained by parabolic flight

Development of a legume root nodule is a complex process culminating in a plant/bacterial symbiosis possessing the capacity for biological dinitrogen fixation. Formation of root nodules is initiated by the binding and stabilization of rhizobia to plant root hairs, mediated in part by a receptor/ligand recognition system composed of lectins on the plant root surface and lectin-binding sites on the rhizobial cell surface. The dinitrogen fixation activity of these root nodules may be an important feature of enclosed, space-based life support systems, and may provide an ecological method to recycle nitrogen for amino acid production. However, the effects on nodule development of varied gravitational fields, or of root nutrient delivery hardware, remain unknown. We have investigated the effects of microgravity on root nodule formation, with preliminary experiments focused upon the receptor/ligand component. Microgravity, obtained during parabolic flight aboard NASA 930, has no apparent effect on the binding of purified lectin to rhizobia, a result that will facilitate forthcoming experiments using intact root tissues.

manned↗
DOE OSTI2020

A historical and comparative review of 50 years of root data collection in Puerto Rico

Fine roots play an important role in plant nutrition, as well as in carbon, water, and nutrient cycling. Fine roots account for a third of terrestrial net primary production (NPP), and inclusion of their structure and function in global carbon models should improve predictions of ecosystem responses to climate change. However, studies focusing on underground plant components are much less frequent than those on aboveground structure. This is more marked in the tropics, where one-third of the planet's terrestrial NPP is produced. Some tropical forests have been more represented in the literature than others, as demonstrated in the collective studies in Puerto Rico. This Caribbean island's biodiversity, frequency of natural disturbances, ease of access to forests, and long-term plots have created an ideal place for the study of tropical ecological processes. This review of the literature emphasizes 50 years of root research and patterns revealed around Puerto Rico. The data in this review were compiled from scientific publications, conference reports, symposiums, and raw data shared by some researches. Emergent patterns include the shallow distribution of fine roots, the great variation in root biomass among different forest types, little variation in root phosphorus concentrations, the slow recovery of root biomass after Hurricane Hugo, and the fact that most data on roots come from the wet tropical Luquillo Experimental Forest, causing other habitat types to be underrepresented. This review also shows the gaps in knowledge about fine roots in the island's ecosystems, which should be used to promote and guide future studies. Abstract in Spanish is available with online material.

tropics↗
DOE OSTI2021

Time-lapse approach to correct deficiencies of 2D soil zymography

Membrane zymography is commonly used in rhizosphere ecology to map enzyme activities in intact soil samples and plant roots. The method consists of incubating a membrane saturated with an enzyme-specific fluorogenic substrate on the soil/root surface followed by measurements of fluorescence intensity of the product in the membrane. The traditional zymography is based on assumptions that fluorescence on membrane images is linearly increasing with time during zymography and an increase in product content is numerically equal to enzyme activity in soil below the membrane. These assumptions are unlikely to hold in experimental settings. In this work we introduce a new zymography technique, time-lapse zymography (TLZ); the approach that eliminates the need for assumptions of the traditional zymography and provides more realistic estimates of enzymatic activities. We assessed the new technique in a series of laboratory and modeling experiments, including quantification of the fluorescent product diffusion (e.g. MUF: 4-methylumbelliferone) from plant roots, enzyme activity measurements on the roots, and HYDRUS-2D & HP2 software calibration with obtained data. The calibrated model was used to analyze the processes governing spatial and temporal dynamics of MUF contents in the membrane. The results indicated that the enzyme diffusion within the membrane-soil system was negligible, and measured zymograms were adequately reproduced solely by accounting for substrate and product diffusions and for catalytic enzyme reaction described by the Michaelis-Menten equation. TLZ enabled identifying and using linear parts on MUF time series and accounting for MUF losses in each zymogram pixel, considerably improving the accuracy as compared to the traditional method. Results demonstrated that enzymatic activity from only a thin soil layer (~0.2 mm) is adequately represented in zymograms.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Community RNA-Seq: multi-kingdom responses to living versus decaying roots in soil

Abstract Roots are a primary source of organic carbon input in most soils. The consumption of living and detrital root inputs involves multi-trophic processes and multiple kingdoms of microbial life, but typical microbial ecology studies focus on only one or two major lineages. We used Illumina shotgun RNA sequencing to conduct PCR-independent SSU rRNA community analysis (“community RNA-Seq”) and simultaneously assess the bacteria, archaea, fungi, and microfauna surrounding both living and decomposing roots of the annual grass, Avena fatua. Plants were grown in 13CO2-labeled microcosms amended with 15N-root litter to identify the preferences of rhizosphere organisms for root exudates (13C) versus decaying root biomass (15N) using NanoSIMS microarray imaging (Chip-SIP). When litter was available, rhizosphere and bulk soil had significantly more Amoebozoa, which are potentially important yet often overlooked top-down drivers of detritusphere community dynamics and nutrient cycling. Bulk soil containing litter was depleted in Actinobacteria but had significantly more Bacteroidetes and Proteobacteria. While Actinobacteria were abundant in the rhizosphere, Chip-SIP showed Actinobacteria preferentially incorporated litter relative to root exudates, indicating this group’s more prominent role in detritus elemental cycling in the rhizosphere. Our results emphasize that decomposition is a multi-trophic process involving complex interactions, and our methodology can be used to track the trajectory of carbon through multi-kingdom soil food webs.

Nuccio, Erin E. (ORCID:000000030189183X)↗
NASA NTRS1992

Growing root, tuber and nut crops hydroponically for CELSS

Among the crops selected by NASA for growth in controlled ecological life-support systems are four that have subsurface edible parts: potatoes, sweet potatoes, sugar beets and peanuts. These crops can be produced in open and closed (recirculating), solid media and liquid, hydroponic systems. Fluorescent, fluorescent plus incandescent, and high-pressure sodium-plus-metal-halide lamps have proven to be effective light sources. Continuous light with 16-C and 28/22-C (day/night) temperatures produce highest yields for potato and sweet potato, respectively. Dry weight yields of up to 4685, 2541, 1151 and 207 g/sq m for potatoes, sweet potatoes, sugar beets and peanuts, respectively, are produced in controlled environment hydroponic systems.

Hill, W. A.↗
DOE OSTI2020

The rhizosphere and cropping system, but not arbuscular mycorrhizae, affect ammonia oxidizing archaea and bacteria abundances in two agricultural soils

Arbuscular mycorrhizal fungi (AMF) form symbioses with roots that can enhance plant nutrition. While AMF have been shown to have a role in soil nitrogen (N) cycling, it is unclear whether AMF affect N cycling microbes such as ammonia-oxidizing bacteria (AOB) and archaea (AOA), which convert ammonium into nitrite in the first step of nitrification. In this study, we examined the effects of AMF on AOA and AOB abundances within the corn rhizosphere and bulk soil of conventional (corn-soybean rotation with inorganic fertilizer) and diversified (cornsoybean- oats/alfalfa-oats rotation with composted manure) systems. We hypothesized that AMF would decrease AOA and AOB abundances in a cropping-system dependent manner, possibly due to competition for ammonium. We grew corn deficient or proficient in AMF symbiosis in microcosms for 10 weeks. At the end of the experiment, both soils planted with the AMF-proficient corn genotype had higher ammonium and lower nitrate pool sizes compared to the same soils planted with the AMF-deficient corn genotype. Likewise, total plant N was higher in the AMF-proficient genotype compared to the AMF-deficient genotype. Despite changes in soil inorganic N pool sizes, AOA and AOB abundances were unaffected by plant AMF-proficiency. Instead, AOA abundance was greater in the rhizosphere than in the bulk soil regardless of cropping system, and AOB abundance was greater in the conventional than the diversified cropping system soil regardless of proximity to the root. These data indicate that 1) AMF did not affect AOA or AOB abundance in these N-rich soils but other factors such as root proximity and inorganic fertilization did and 2) AOA and AOB have differing ecological niches within rhizosphere and bulk soil that should be considered when managing for nitrogen losses.

Wattenburger, Cassandra↗
DOE OSTI2025

Dual-mycorrhizal colonization is determined by plant age and host identity in two species of Populus

Plants have evolved symbioses with mycorrhizal and endophytic fungi that are essential for their growth and survival. While most plants associate with a single guild of mycorrhizal fungi, a select group termed “dual-mycorrhizal plants” associate with both arbuscular mycorrhizal and ectomycorrhizal fungi. Although a shift from predominance of arbuscular mycorrhizal to ectomycorrhizal colonization with plant development has been demonstrated on other dual-mycorrhizal hosts, it is not known how mycorrhizal colonization shifts with plant age in Populus species. We performed a controlled growth experiment with natural field-sourced inocula to test for age-dependent shifts in fungal colonization rates and for host-specific patterns of colonization in two species of Populus (P. tremuloides and P. trichocarpa). We found that only P. trichocarpa displayed dual-mycorrhizal colonization, while P. tremuloides associated with ectomycorrhizal fungi, but not arbuscular mycorrhizal fungi. Both guilds of mycorrhizal fungi increased in abundance with plant age, while root endophytic fungal colonization decreased. Many of the early-colonizing endophytic fungi that we documented have strong saprotrophic capabilities, which may be an important trait for fast colonization. Dark septate endophytes were more abundant than either guild of mycorrhizal fungi, and are likely to be functionally important members of the Populus root fungal community. Our findings represent a novel pattern in the development of dual-mycorrhizal colonization and illustrate that Populus species vary in their association with arbuscular mycorrhizal fungi. In conclusion, our results also highlight the importance of dark septate endophyte colonization dynamics on dual-mycorrhizal plants.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1989

Effect of channel size on sweet potato storage root enlargement in the Tuskegee University hydroponic nutrient film system

The potential of the sweet potato as a food source for future long term manned space missions is being evaluated for NASA's Controlled Ecological Life Support Systems (CELSS) program. Sweet potatoes have been successfully grown in a specially designed Tuskegee University nutrient film technique (TU NFT) system. This hydroponic system yielded storage roots as high as 1790 g/plant fresh weight. In order to determine the effect of channel size on the yield of sweet potatoes, the width and depth of the growing channels were varied in two separate experiments. Widths were studied using the rectangular TU NFT channels with widths of 15 cm (6 in), 30 cm (12 in) and 45 cm (18 in). Channel depths of 5 cm (2 in), 10 cm (4 in), and 15 cm (6 in) were studied using a standard NASA fan shaped Biomass Production Chamber (BPC) channel. A comparison of preliminary results indicated that, except for storage root number, the growth and yield of sweet potatoes were not affected by channel width. Storage root yield was affected by channel depth although storage root number and foliage growth were not. Both experiments are being repeated.

Morris, Carlton E.↗
DOE OSTI2025

Vulnerability of mineral-organic associations in the rhizosphere

The majority of soil carbon (C) is stored in organic matter associated with reactive minerals. These mineral-organic associations (MOAs) inhibit microbial and enzymatic access to organic matter, suggesting that organic C within MOAs is resistant to decomposition. However, plant roots and rhizosphere microbes are known to transform minerals through dissolution and exchange reactions, implying that MOAs in the rhizosphere can be dynamic. Here we identify key drivers, mechanisms, and controls of MOA disruption in the rhizosphere and present a new conceptual framework for the vulnerability of soil C within MOAs. We introduce a vulnerability spectrum that highlights how MOAs characteristic of certain ecosystems are particularly susceptible to specific root-driven disruption mechanisms. This vulnerability spectrum provides a framework for critically assessing the importance of MOA disruption mechanisms at the ecosystem scale. Comprehensive representation of not only root-driven MOA formation, but also disruption, will improve model projections of soil C-climate feedbacks and guide the development of more effective soil C management strategies.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRSDate not supplied

Validation of Remotely Sensed and Modeled Soil Moisture at Forested and Unforested Sites

Soil moisture is an important driver for forest ecosystems, influencing fire occurrence and extent, insect and pathogen impacts, and tree growth, which creates a need for regular, globally extensive soil moisture information that only satellite-based sensors or models can achieve. However, the reliability of soil moisture measurements in forests is not well understood due to a lack of suitable validation sites (especially relative to unforested ecosystems) and interference caused by high vegetation water content on remotely sensed measurements; although recent studies have started to address this gap [1], [2], [3], [4]. Here we validate the performance of multiyear remotely sensed (SMAP/Sentinel), remotely sensed data assimilation modelled (SMAP-L4), and modelled (NLDAS) surface and root zone (0-1 m) soil moisture datasets with data from in-situ sensors at 39 National Ecological Observatory Network (NEON) sites throughout the contiguous US. Due to differences in spatial resolution, NEON soil moisture (~0.2 km measurement zone) correlations were expected to be stronger with the SMAP/Sentinel product (3 km resolution) than with coarser resolution SMAP-L4 (9 km resolution) or NLDAS products (13 km resolution). However, given the sensitivity of satellite measurements to vegetation water content we expected a deterioration in the correlations based on remotely sensed measurements (SMAP/Sentinel and SMAP-L4) as aboveground biomass increased, whereas the model-based data (NLDAS) was expected to be largely insensitive to vegetation type. We recognize that the SMAP/Sentinel product was developed for unforested regions, therefore our application is outside its primary use case. Soil moisture is measured at up to 8 depths in five soil plots spaced up to 40 m apart at each NEON terrestrial site. Correlation parameters were calculated for the three remotely sensed and modelled data products relative to in-situ measurement following Entekhabi et al. [5]. The datasets comprised 94 (SMAP-L4), 28 (SMAP/Sentinel), and 106 (NLDAS) sites-years for surface soils and 13 (SMAP-L4) and 14 (NLDAS) site-years for the root zone. At unforested sites, the performance of the three remotely sensed and modelled data products was similar for surface soils (Table 1). For example, unbiased RMSD (ubRMSD), which SMAP uses as its primary performance metric [6], ranged from 0.05 to 0.06 m3 m-3 (Table 1), indicating the ability of all three products to track changes in soil moisture over time. The performance of the three products deteriorated at forested sites, however, while the difference in performance was modest for SMAP-L4 and NLDAS, the deterioration in SMAP/Sentinel performance was substantial. For instance, SMAP/Sentinel ubRMSD increased from 0.06 to 0.11 m3 m-3 and absolute mean difference (Abs MD; which includes measurement bias and spatial representativeness errors) increased from 0.06 to 0.16 m3 m-3, indicating both a reduction in ability to track temporal changes and absolute amounts of soil moisture in forest ecosystems. SMAP-L4 and NLDAS had lower unbiased RMSD for root zone (0-1 m) than surface soils at both forested and unforested sites (Tables 1 and 2; SMAP/Sentinel does not produce a root zone measurement). However, in most cases the correlation coefficient (r) was lower for the root zone than surface soils, suggesting the lower unbiased RMSD may be attributed to greater temporal stability of soil moisture in the root zone rather than improved data product performance. Mean difference and absolute mean difference, which encompass measurement bias and spatial representativeness errors, were greater for root zone than surface soils at unforested sites for both data products, but the opposite was generally true at forested sites. As with surface soils, there was relatively little change in the performance of SMAP-L4 and NLDAS between the unforested and forested sites. In summary, all three data products were able to adequately represent soil moisture at unforested sites, at least when aggregating across sites. However, while the performance of all three products deteriorated at forested sites, SMAP-L4 and NLDAS maintained sufficient performance to remain suitable for some use cases (ubRMSD <0.06 m3 m-3 and RMSD <0.13 m3 m-3). In contrast, the relatively poorer performance of the SMAP/Sentinel product at forested sites seems insufficient for most use cases (ubRMSD >0.1 m3 m-3 and RMSD >0.2 m3 m-3). We attribute the large reduction in the performance of the SMAP/Sentinel product in forests to its use of C-band wavelengths, which are particularly sensitive to vegetation interference, and apparently outweighed any gains provided by its higher spatial resolution. A combined SMAP/NISAR soil moisture product may provide improved performance relative to SMAP/Sentinel due to NISAR’s use of L-band wavelengths, which are less sensitive to vegetation (NISAR is scheduled for launch in early 2024).

Edward Ayres↗
DOE OSTI2024

The Largely Ignored but Important Role of Non‐Rooted, Submersed Vegetation and Small Waterbodies on Greenhouse Gas Emissions

Abstract Small, shallow waterbodies are abundant globally and are associated with greenhouse gas (GHG) emissions disproportionate to their size. One reason for this is the prevalence of non‐rooted, submersed (NRS) macrophytes that often dominate these systems. Despite these plants' significant role in the carbon ecology of their ecosystems, they are often excluded from climate change models due, in part, to the lack of clarity of their effects. Theus et al. (2023, https://doi.org/10.1029/2023JG007758 ) provides new insights into the role that NRS macrophytes have on GHG emissions, demonstrating that increasing densities of these macrophytes lead to increases in both CO 2 and CH 4 gas fluxes. Here, we highlight the importance of both shallow waterbodies and NRS vegetation within the context of the field of aquatic ecosystem biogeochemistry. We identify a need for the inclusion of these drivers into climate change models based partly on the findings of Theus et al. (2023, https://doi.org/10.1029/2023JG007758 ).

Hassett, Erin↗
DOE OSTI2026

metagRoot: a comprehensive database of protein families associated with plant root microbiomes

The plant root microbiome is vital in plant health, nutrient uptake, and environmental resilience. To explore and harness this diversity, we present metagRoot, a specialized and enriched database focused on the protein families of the plant root microbiome. MetagRoot integrates metagenomic, metatranscriptomic, and reference genome-derived protein data to characterize 71 091 enriched protein families, each containing at least 100 sequences. These families are annotated with multiple sequence alignments, CRISPR elements, hidden Markov models, taxonomic and functional classifications, ecosystem and geolocation metadata, and predicted 3D structures using AlphaFold2. MetagRoot is a powerful tool for decoding the molecular landscape of root-associated microbial communities and advancing microbiome-informed agricultural practices by enriching protein family information with ecological and structural context. The database is available at https://pavlopoulos-lab.org/metagroot/ or https://www.metagroot.org.

Chasapi, Maria N↗
DOE OSTI2022

Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress

Abstract We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these questions include the need to better understand how plants detect water availability, temperature, salinity, and rising carbon dioxide (CO2) levels; how environmental signals interface with endogenous signaling and development (e.g. circadian clock and flowering time); and how this integrated signaling controls downstream responses (e.g. stomatal regulation, proline metabolism, and growth versus defense balance). The plasma membrane comes up frequently as a site of key signaling and transport events (e.g. mechanosensing and lipid-derived signaling, aquaporins). Adaptation to water extremes and rising CO2 affects hydraulic architecture and transpiration, as well as root and shoot growth and morphology, in ways not fully understood. Environmental adaptation involves tradeoffs that limit ecological distribution and crop resilience in the face of changing and increasingly unpredictable environments. Exploration of plant diversity within and among species can help us know which of these tradeoffs represent fundamental limits and which ones can be circumvented by bringing new trait combinations together. Better defining what constitutes beneficial stress resistance in different contexts and making connections between genes and phenotypes, and between laboratory and field observations, are overarching challenges.

Biochemistry & Molecular Biology↗