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At least 55 records · Page 3

Bi-directional streaming of solar wind electrons greater than 80 eV - ISEE evidence for a closed-field structure within the driver gas of an interplanetary shock

In near time coincidence with the arrival of helium enriched plasma driving the shock wave disturbance of November 12-13, 1978, strong bi-directional streaming of solar wind electrons greater than about 80 eV was observed with Los Alamos instrumentation on ISEE 3. The streaming persisted for many hours simultaneously parallel and anti-parallel to the interplanetary magnetic field which was directed roughly perpendicular to the sun-satellite line. This example of bidirectional streaming cannot be explained by field line connection to the earth's bow shock or the outward propagating interplanetary shock which passed ISEE 3 approximately 16 hours earlier. The event is explained if the local interplanetary field was a part of a magnetic bottle rooted at the sun or a disconnected loop propagating outward.

Bame, S. J.↗

Ionic signaling in plant gravity and touch responses

Plant roots are optimized to exploit resources from the soil and as each root explores this environment it will encounter a range of biotic and abiotic stimuli to which it must respond. Therefore, each root must possess a sensory array capable of monitoring and integrating these diverse stimuli to direct the appropriate growth response. Touch and gravity represent two of the biophysical stimuli that plants must integrate. As sensing both of these signals requires mechano-transduction of biophysical forces to biochemical signaling events, it is likely that they share signal transduction elements. These common signaling components may allow for cross-talk and so integration of thigmotropic and gravitropic responses. Indeed, signal transduction events in both plant touch and gravity sensing are thought to include Ca(2+)- and pH-dependent events. Additionally, it seems clear that the systems responsible for root touch and gravity response interact to generate an integrated growth response. Thus, primary and lateral roots of Arabidopsis respond to mechanical stimuli by eliciting tropic growth that is likely part of a growth strategy employed by the root to circumvent obstacles in the soil. Also, the mechano-signaling induced by encountering an obstacle apparently down-regulates the graviperception machinery to allow this kind of avoidance response. The challenge for future research will be to define how the cellular signaling events in the root cap facilitate this signal integration and growth regulation. In addition, whether other stimuli are likewise integrated with the graviresponse via signal transduction system cross-talk is an important question that remains to be answered.

Review↗

Cortical and cap sedimentation in gravitropic Equisetum roots

Although the rootcap is required for gravitropic sensing, various classical and contemporary data raise the question of whether additional sensing occurs away from the cap in roots. Roots of Equisetum hyemale L. (horsetail) were examined by light and electron microscopy to determine which cell components were distributed with respect to gravity both in and away from the rootcap. Adventitious roots from stem cuttings were gravitropic in a vertical orientation or if reoriented to the horizontal. Obvious amyloplast sedimentation was found in vertical and in reoriented roots 1) in cells in the center of the rootcap and 2) in young, elongating cortical cells located in two to three layers outside the endodermis. These cortical amyloplasts were smaller than cap amyloplasts and, unlike central cap amyloplasts, were occasionally found in the top of the cell. The nucleus was also sedimented on top of the amyloplasts in both cell types, both in vertical and in reoriented roots. Sedimentation of both organelles ceased as cortical cells elongated further or as cap cells became peripheral in location. In both cell types with sedimentation, endoplasmic reticulum was located in the cell periphery, but showed no obvious enrichment near the lower part of the cell in vertical roots. This is the first modern report of sedimentation away from the cap in roots, and it provides structural evidence that gravitropic sensing may not be confined to the cap in all roots.

NASA Discipline Plant Biology↗

Assessing the Effect of a Deep‐Rooted Grass on Belowground Carbon Storage in Cultivated Land: Insights From a Multi‐Site US Study

Agriculture depletes soil organic carbon (SOC), partly due to the exclusion of deep-rooted perennials. Reintroducing deep-rooted perennials to cultivated land may help to mitigate SOC loss. We quantified the effect of deep roots on SOC by comparing 8 to 30 year-old stands of switchgrass ( Panicum virgatum L .) with paired annual row crop fields at 12 sites across the central and eastern USA. We hypothesized that switchgrass would store more root C and SOC than neighboring shallow-rooted annual crops, and that these effects would extend deeper than 30 cm. We also evaluated whether switchgrass stimulates decomposition of SOC at depth using radiocarbon ( 14 C). Finally, we explored whether the effect of switchgrass on SOC is moderated by soil chemical and physical properties. While the effect of switchgrass on SOC in the surface 100 cm was positive at most sites, the average effect was not statistically significant (difference in SOC = 0.6 kg C m −2 [95% CI −0.8 to +1.9 kg C m −2 ]). By contrast, we found that root C was consistently more abundant under switchgrass, yielding an estimated additional 0.6 kg C m −2 in the surface 100 cm of soil [95% CI +0.5 to +0.7 kg C m −2 ]. 14 C measurements suggested that root C inputs were adding to existing SOC without stimulating decomposition. The effect of switchgrass on belowground C was not strongly related to any of the soil properties that we evaluated. Our observations show that root C can contribute substantially to belowground C stocks when deep-rooted perennials replace shallow-rooted crops.

60 APPLIED LIFE SCIENCES↗

Arabidopsis alcohol dehydrogenase expression in both shoots and roots is conditioned by root growth environment

It is widely accepted that the Arabidopsis Adh (alcohol dehydrogenase) gene is constitutively expressed at low levels in the roots of young plants grown on agar media, and that the expression level is greatly induced by anoxic or hypoxic stresses. We questioned whether the agar medium itself created an anaerobic environment for the roots upon their growing into the gel. beta-Glucuronidase (GUS) expression driven by the Adh promoter was examined by growing transgenic Arabidopsis plants in different growing systems. Whereas roots grown on horizontal-positioned plates showed high Adh/GUS expression levels, roots from vertical-positioned plates had no Adh/GUS expression. Additional results indicate that growth on vertical plates closely mimics the Adh/GUS expression observed for soil-grown seedlings, and that growth on horizontal plates results in induction of high Adh/GUS expression that is consistent with hypoxic or anoxic conditions within the agar of the root zone. Adh/GUS expression in the shoot apex is also highly induced by root penetration of the agar medium. This induction of Adh/GUS in shoot apex and roots is due, at least in part, to mechanisms involving Ca2+ signal transduction.

NASA Discipline Plant Biology↗

Root Influences on Mobilization and Export of Mineral-bound Soil Organic Matter

Biogeochemical cycles within mountainous watersheds are key regulators of ecosystem carbon storage and downstream nutrient loadings, and they have shown to be particularly vulnerable to climate change impacts. Increasing temperature and persistent droughts have already dramatically changed vegetation cover across the mountainous western US, with unknown consequences for soil carbon and nutrient cycles belowground. What remains elusive is to what extent associated changes in root-soil interactions may mobilize the vast pool of organic matter (OM) that has been stabilized by associations with minerals for centuries or millennia. Although plant root-driven OM mobilization from minerals may be a central control on carbon loss and nutrient export, such mechanisms are currently missing from conceptual and numerical models. The overall objective of this Exploratory Project is to identify the biogeochemical mechanisms by which roots destabilize mineral-associated organic matter (MAOM) and the cumulative impact on carbon and nutrient fate. To accomplish this goal, we integrated well-controlled laboratory experiments with in-field measurements and a scalable modeling approach. First, we conducted model system experiments to assess the vulnerability of MAOM to exudate-mediated mechanisms. Our results show that common root exudates effectively destabilize MAOM not only through direct, ligand-driven mobilization mechanisms, but also indirect, microbially-mediated mechanisms relying on secondary metabolites and enzymes. We further found that OM bound to poorly crystalline Fe and Al (hydr)oxides is more vulnerable to exudate-induced destabilization than OM bound to more crystalline phases, particularly in response to direct, ligand-promoted mechanisms. These findings demonstrate that the stability of MAOM is not just a function of their inherent properties, but also will depend in large parts on the ability of plant roots and microbes to produce exudates capable of triggering suitable mobilization mechanisms. We further employed a well-controlled rhizobox approach, combining advanced microsensor and mass spectrometry techniques, to resolve spatiotemporal variations in the composition and availability of exudates along single growing roots of grasses. Our results show that the composition of functionally relevant exudate compounds varies at extremely short time scales, seemingly shifting from ligands such as aromatic acids around root tips to less reactive metabolites such as amino acids around mature root segments. These results suggest a prevalence of direct MAOM mobilization mechanisms around the root tip, while indirect MAOM mobilization strategies may dominate around more mature root segments. Additionally, we utilized microsensor measurements in rhizoboxes to parameterize a rhizosphere (hydro)biogeochemistry reactive transport model (eSTOMP-ROOTS). The resulting model was used to assess how root exudations affects the stability of MAOM. Model simulations show that diel pulses of root exudation are strong enough to cause oscillations in biogeochemical conditions—particularly in pH, oxygen concentrations, and microbial activity— that repeatedly disrupt MAOM. Finally, we assessed field-scale impacts of root-driven MAOM destabilization over short and long time scales. A combination of in-field incubations and field-based characterizations showed that MAOM may not only be vulnerable to seasonal variations in root activity, but also to root-driven weathering over pedogenic time scales. In sum, results from our Exploratory Award highlight the strong control plant roots exert on MAOM mobilization and, thus, on the potential for carbon and nutrient export from watersheds.

54 ENVIRONMENTAL SCIENCES↗

Assimilation of GRACE Terrestrial Water Storage into a Land Surface Model: Evaluation 1 and Potential Value for Drought Monitoring in Western and Central Europe

A land surface model s ability to simulate states (e.g., soil moisture) and fluxes (e.g., runoff) is limited by uncertainties in meteorological forcing and parameter inputs as well as inadequacies in model physics. In this study, anomalies of terrestrial water storage (TWS) observed by the Gravity Recovery and Climate Experiment (GRACE) satellite mission were assimilated into the NASA Catchment land surface model in western and central Europe for a 7-year period, using a previously developed ensemble Kalman smoother. GRACE data assimilation led to improved runoff correlations with gauge data in 17 out of 18 hydrological basins, even in basins smaller than the effective resolution of GRACE. Improvements in root zone soil moisture were less conclusive, partly due to the shortness of the in situ data record. In addition to improving temporal correlations, GRACE data assimilation also reduced increasing trends in simulated monthly TWS and runoff associated with increasing rates of precipitation. GRACE assimilated root zone soil moisture and TWS fields exhibited significant changes in their dryness rankings relative to those without data assimilation, suggesting that GRACE data assimilation could have a substantial impact on drought monitoring. Signals of drought in GRACE TWS correlated well with MODIS Normalized Difference Vegetation Index (NDVI) data in most areas. Although they detected the same droughts during warm seasons, drought signatures in GRACE derived TWS exhibited greater persistence than those in NDVI throughout all seasons, in part due to limitations associated with the seasonality of vegetation.

Li, Bailing↗

Microsurgical removal of epidermal and cortical cells: evidence that the gravitropic signal moves through the outer cell layers in primary roots of maize

There is general agreement that during root gravitropism some sort of growth-modifying signal moves from the cap to the elongation zone and that this signal ultimately induces the curvature that leads to reorientation of the root. However, there is disagreement regarding both the nature of the signal and the pathway of its movement from the root cap to the elongation zone. We examined the pathway of movement by testing gravitropism in primary roots of maize (Zea mays L.) from which narrow (0.5 mm) rings of epidermal and cortical tissue were surgically removed from various positions within the elongation zone. When roots were girdled in the apical part of the elongation zone gravitropic curvature occurred apical to the girdle but not basal to the girdle. Filling the girdle with agar allowed curvature basal to the girdle to occur. Shallow girdles, in which only two or three cell layers (epidermis plus one or two cortical cell layers) were removed, prevented or greatly delayed gravitropic curvature basal to the girdle. The results indicate that the gravitropic signal moves basipetally through the outermost cell layers, perhaps through the epidermis itself.

NASA Discipline Number 29-20↗

Greenhouse Rhizobox Experiment with Plant Characteristics, Porewater, Gas Flux, and Soil Biogeochemistry data, Seward Peninsula, Alaska, 2024

Data collected from a greenhouse rhizobox experiment (2024) using soils and plants collected at Council, AK (64°51’35.0”N 163°41’59.1”W) during a summer campaign in 2023. Water data consists of soil porewater collected by porewater samplers (rhizons). Gas data consists of CO2 and CH4 surface soil fluxes measured with an FTIR (Fourier-transformed infrared red) analyzer. Plant and root data consists of biomass, root length. This study is a part of The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).This dataset was generated to broadly address the following research question: how will climate change (i.e., thawing permafrost, landscape change) alter the ecosystem flux (sink versus source) of important greenhouse gases such as CO2 and CH4?Description of the contents of this data package: Rhizobox2024_Data.csv: This dataset contains plant, water and gas data. No software is needed to utilize them.nga535_flmd.csv: The file contains file level metadatanga535.dd.csv: This file contains the data dictionaryMethods.pdf: This file contains the data collection methods

54 ENVIRONMENTAL SCIENCES↗

HL-LHC Analysis With ROOT: ROOT Project Input to the HL-LHC Computing Review (Stage 2)

ROOT is high energy physics' software for storing and mining data in a statistically sound way, to publish results with scientific graphics. It is evolving since 25 years, now providing the storage format for more than one exabyte of data; virtually all high energy physics experiments use ROOT. With another significant increase in the amount of data to be handled scheduled to arrive in 2027, ROOT is preparing for a massive upgrade of its core ingredients. As part of a review of crucial software for high energy physics, the ROOT team has documented its R&D plans for the coming years.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Automated Airborne Pathogen Monitoring for Agriculture (CRADA Final Report)

As part of the Cyclotron Road program, Root Applied Sciences investigated the use of DNA-based assays under field conditions to detect airborne plant pathogens in environmental samples. Robust DNA-based assays are critical for automated monitoring of plant pathogen concentrations in the air using Root’s air samplers. A fully automated air sampler coupled with DNA-based assays capable of operating under field conditions will accelerate the delivery of disease risk alerts based on airborne inoculum loads. Timely and accurate alerts of pathogen loads in the air can help growers manage airborne diseases more precisely, avoiding fungicide applications when there is no threat, and focusing cultural practices in the right areas. This project built upon other work done by Root to study the in-field performance of a liquid DNA-based assay for detection of grape powdery mildew. Growers working with Root’s airborne powdery mildew monitoring system have reported 20-80% reductions in pesticides.

60 APPLIED LIFE SCIENCES↗

Viewing Welds By Computer Tomography

Computer tomography system used to inspect welds for root penetration. Source illuminates rotating welded part with fan-shaped beam of x rays or gamma rays. Detectors in circular array on opposite side of part intercept beam and convert it into electrical signals. Computer processes signals into image of cross section of weld. Image displayed on video monitor. System offers only nondestructive way to check penetration from outside when inner surfaces inaccessible.

Pascua, Antonio G.↗

Fracture Mechanics Analyses of the Slip-Side Joggle Regions of Wing-Leading-Edge Panels

The Space Shuttle wing-leading edge consists of panels that are made of reinforced carbon-carbon. Coating spallation was observed near the slip-side region of the panels that experience extreme heating. To understand this phenomenon, a root-cause investigation was conducted. As part of that investigation, fracture mechanics analyses of the slip-side joggle regions of the hot panels were conducted. This paper presents an overview of the fracture mechanics analyses.

Raju, Ivatury S.↗

Improving operations: Metrics to Results

As a result of the mission failure of the Mars Climate Orbiter (MCO) spacecraft in 1999, the Jet Propulsion Laboratory (JPL) initiated the development of a Mission Operations Assurance (MOA) program to be implemented across all flight projects managed by JPL. One of the initiatives undertaken in 2001 was the collection of data on command file errors occurring in the operational phase of the mission. This paper defines command file errors and how and where they occur in the operations process. It also describes the problem reporting system (PRS) in use for mission operations at JPL. We examine the recent modifications to the PRS that enable the collection of metrics, specifically on command file errors. This paper discusses what the data show us since metrics have been collected for the operational missions conducted by JPL. We examine the evolution of an operational working group initiative to evaluate proximate, contributing, and root causes for the errors. As part of this discussion we see what the metrics have indicated over a decade. At the macro level, we can say that the aggregate command file error rate has been cut to roughly one third of the initial 2001 level by the end of 2011. Additionally, we explore efficient and innovative means to continually integrate the findings and recommendations from the working group back into the flight operations environment.

command file errors↗

A Discussion of the Failure of a Quad Diode Module and Efforts to Assure the Flight Spares

In 2019 the International Space Station (ISS) experienced an on on-orbit failure that affected 1 of 28 Battery Charge Discharge Units (BCDUs). Telemetry pointed to a short circuit failure of a Power Rectifier Quad Diode Module. Astronauts removed the failed unit from service which was then returned to Earth for failure analysis. The failure analysis confirmed that the quad diode module had failed short circuit. The investigation identified silver dendrites had grown on the insulated, sloped edge of the mesa semiconductor die of the failed diode and also a 2nd diode in the same quad diode module. Voids between the diode’s protective encapsulating ring and the die provided space within which dendrites were able to form and cause catastrophic failure. The NASA Engineering & Safety Center (NESC) convened a EEE Parts Sub-Team to investigate root cause and to assist with risk assessment for all of the flight diode modules (4 distinct production lots) and the flight spares (from a 5th lot). Analysis of the original diode manufacturer’s read and record screening test data identified some lots which contained diodes with parametric instabilities that could signal conditions suited for silver dendrite formation. Computed Tomography (CT) X-ray screening inspection was performed on flight spare modules to identify diodes that do not exhibit voids between the edge of die and encapsulating ring as a mitigation against dendrite formation.

Jay Brusse↗

Aerojet - Attitude Control Engines

All the engines were both qualification and acceptance tested at Marquardt s facilities. After we won the Apollo Program contract, we went off and built two vacuum test facilities, which simulated altitude continuous firing for as long as we wanted to run an engine. They would run days and days with the same capability we had on steam ejection. We did all of the testing in both for the qualification and the acceptance test. One of them was a large ball, which was an eighteen-foot diameter sphere, evacuated again with a big steam ejector system that could be used for system testing; that s where we did the Lunar Excursion Module testing. We put the whole cluster in there and tested the entire cluster at the simulated altitude conditions. The lowest altitude we tested at - typically an acceptance test - was 105,000 feet simulated altitude. The big ball - because people were interested in what they called goop formation, which is an unburned hydrazine product migrating to cold surfaces on different parts of spacecraft - was built to address those kinds of issues. We ran long-life tests in a simulated space environment with the entire inside of the test cell around the test article, liquid nitrogen cooled, so it could act as getter for any of the exhaust products. That particular facility could pull down to about 350,000 feet (atmosphere) equivalent altitude, which was pushing pretty close to the thermodynamic triple point of the MMH. It was a good test facility. Those facilities are no longer there. When the guys at Marquardt sold the company to what eventually became part of Aerojet, all those test facilities were cut off at the roots. I think they have a movie studio there at this point. That part of it is truly not recoverable, but it did some excellent high-altitude, space-equivalent testing at the time. Surprisingly, we had very few problems while testing in the San Fernando Valley. In the early 1960s, nobody had ever seen dinitrogen tetroxide (N2O4), so that wasn't too big a deal. We really did only make small, red clouds. In all the hundreds of thousands of tests and probably well over one million firings that I was around that place for, in all that thirty-something years, we had a total of one serious injury associated with rocket engine testing and propellants. Because we were trying to figure out what propellants would really be good, we tried all of the fun stuff like the carbon tetrafluoride, chlorine pentafluoride, and pure fluorine. The materials knowledge wasn't all that great at the time. On one test, the fluorine we had didn't react well with the copper they were using for tubing, and it managed to cause another unscheduled disassembly of the facility. It was very serious. It's like one of those Korean War stories. The technician happened to be walking past the test facility when it decided to blow itself up. A piece of copper tubing pierced one cheek and came out the other. That was the only serious accident in all of the engines handled in all those years. Now, we did have a problem with the EPA later because they figured out what the brown clouds were about. We built a whole bunch of exhaust mitigation scrubbers to take care of engine testing in the daytime. In general, we operated the big shuttle (RCS) engine, the 870- pounder at nominal conditions; they scrubbed the effluents pretty well. If you operated that same 870-pound force engine at a level where you get a lot of excess oxidizer, yeah, there s a brown cloud. But, you know, it doesn't show up well in the dark. They did do some of that. But, that s gone; it was addressed one way or another. RELEASED -

Pfeifer, Gerald R.↗

Controlled environment crop production - Hydroponic vs. lunar regolith

The potential of controlled environment crop production in a lunar colony is discussed. Findings on the effects of optimal root-zone and aerial environments derived as part of the NASA CELSS project at Utah State are presented. The concept of growing wheat in optimal environment is discussed. It is suggested that genetic engineering might produce the ideal wheat cultivar for CELSS (about 100 mm in height with fewer leaves). The Utah State University hydroponic system is outlined and diagrams of the system and plant container construction are provided. Ratio of plant mass to solution mass, minimum root-zone volume, maintenance, and pH control are discussed. A comparison of liquid hydrophonic systems and lunar regoliths as substrates for plant growth is provided. The physiological processes that are affected by the root-zone environment are discussed including carbon partitioning, nutrient availability, nutrient absorption zones, root-zone oxygen, plant water potential, root-produced hormones, and rhizosphere pH control.

Bugbee, Bruce G.↗

An Approximate Method of Calculating the Deformations of Wings Having Swept, M or W, A, and Swept-Tip Plan Forms

An approximate method of calculating the deformations of wings of uniform thickness having swept, M or W, Delta, and swept-tip plan forms is presented. The method employs an adjustment to the elementary beam theory to account for the effect of the triangular root portion of a swept wing on the deformation of the outboard section of the wing. To demonstrate the general applicability of the method, the modified elementary theory is applied to the more complex M or W, Delta, and swept-tip plan forms as well as to swept plan forms. For the purpose of calculating angles of attack, it is shown that the unmodified elementary beam theory applied to that part of the wing outboard of the root triangle produces satisfactory results. However, for calculating deflections it is necessary to include the effects of the root-triangle deformation.

Zender, George W.↗