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A case report on bilateral partial breast irradiation using SAVI

To assess dosimetric parameters in a case study where bilateral accelerated partial breast irradiation (APBI) is delivered using a strut-adjusted volume implant (SAVI) device. A 59-year-old female received APBI in both breasts over 5 days, with fractions of 3.4 Gy twice daily. A Vac-lok system was used for immobilization, and a C-arm was used for daily imaging. We generated dose-volume histograms (DVHs) for the brachytherapy plans to derive several important biologic factors. We calculated the normal tissue complication probability (NTCP), equivalent uniform dose (EUD), and tumor control probability (TCP) using the Lyman-Kutcher-Burman model parameters {alpha} = 0.3 Gy{sup -1}, {alpha}/{beta} = 4 Gy, n = 0.1, and m = 0.3. In addition, we assessed the dose homogeneity index (DHI), overdose index, and dose nonuniformity ratio. D95 was >95% and V150 was <50 mL for both breasts. The DHIs were 0.469 and 0.512 for the left and right breasts, respectively. The EUDs (normalized to 3.4 Gy b.i.d.) were 33.53 and 29.10 Gy. The TCPs were estimated at 99.2% and 99.9%, whereas the NTCP values were 4.2% and 2.57%. In this clinical case, we were able to quantify the dosimetric parameters of an APBI treatment performed with a SAVI device.

61 RADIATION PROTECTION AND DOSIMETRY↗

Advances in the theory of 0{nu}{beta}{beta} decay

Recent advances in the theory of 0{nu}{beta}{beta} decay are briefly discussed. A complete list of nuclear matrix elements within the framework of the Interacting Boson Model (IBM-2) is given. Results of a novel calculation of phase space factors with exact Dirac wave functions and electron screening are also presented.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

THE z = 5 QUASAR LUMINOSITY FUNCTION FROM SDSS STRIPE 82

We present a measurement of the Type I quasar luminosity function at z = 5 using a large sample of spectroscopically confirmed quasars selected from optical imaging data. We measure the bright end (M{sub 1450} < -26) with Sloan Digital Sky Survey (SDSS) data covering {approx}6000 deg{sup 2}, then extend to lower luminosities (M{sub 1450} < -24) with newly discovered, faint z {approx} 5 quasars selected from 235 deg{sup 2} of deep, coadded imaging in the SDSS Stripe 82 region (the celestial equator in the Southern Galactic Cap). The faint sample includes 14 quasars with spectra obtained as ancillary science targets in the SDSS-III Baryon Oscillation Spectroscopic Survey, and 59 quasars observed at the MMT and Magellan telescopes. We construct a well-defined sample of 4.7 < z < 5.1 quasars that is highly complete, with 73 spectroscopic identifications out of 92 candidates. Our color selection method is also highly efficient: of the 73 spectra obtained, 71 are high-redshift quasars. These observations reach below the break in the luminosity function (M{sub 1450}{sup *}{approx}-27). The bright-end slope is steep ({beta} {approx}< -4), with a constraint of {beta} < -3.1 at 95% confidence. The break luminosity appears to evolve strongly at high redshift, providing an explanation for the flattening of the bright-end slope reported previously. We find a factor of {approx}2 greater decrease in the number density of luminous quasars (M{sub 1450} < -26) from z = 5 to z = 6 than from z = 4 to z = 5, suggesting a more rapid decline in quasar activity at high redshift than found in previous surveys. Our model for the quasar luminosity function predicts that quasars generate {approx}30% of the ionizing photons required to keep hydrogen in the universe ionized at z = 5.

79 ASTRONOMY AND ASTROPHYSICS↗

Solar Flare Abundances of Potassium, Argon, and Sulphur

The absolute coronal abundances of potassium has been determined for the first time from X-ray solar flare line and continuous spectra together with absolute and relative abundances of Ar and S. Potassium is of importance in the continuing debate concerning the nature of the coronal/photospheric element abundance ratios which are widely considered to depend on first ionization potential since it has the lowest FIP of any common element in the Sun. The measurements were obtained with the RESIK crystal spectrometer on the Coronas-F spacecraft. A differential emission measure DEM = const. x exp (-(beta)T(sub e) was found to be the most consistent with the data out of three models considered. We find that the coronal ratio [K/H] = 3.7 x 10(exp - 7), a factor 3 times photospheric, in agreement with other observations using line-to-line ratios. Our measured value for the coronal ratio [Ar/H] = 1.5 x 10(exp -6) is significantly less than photospheric, indicating that there is a slight depletion of this high-FIP element in the corona. For S (an intermediate-FIP element) we obtained [S/H] = 2.2 x 10(exp - 5), approximately the same as in previous work.

Oegerle, William↗

The Changing Face of Vascular Interventional Radiology: The Future Role of Pharmacotherapies and Molecular Imaging

Interventional radiology has had to evolve constantly because there is the ever-present competition and threat from other specialties within medicine, surgery, and research. The development of new technologies, techniques, and therapies is vital to broaden the horizon of interventional radiology and to ensure its continued success in the future. In part, this change will be due to improved chronic disease prevention altering what we treat and in whom. The most important of these strategies are the therapeutic use of statins, Beta-blockers, angiotensin-converting enzyme inhibitors, and substances that interfere with mast cell degeneration. Molecular imaging and therapeutic strategies will move away from conventional techniques and nano and microparticle molecular technology, tissue factor imaging, gene therapy, endothelial progenitor cells, and photodynamic therapy will become an important part of interventional radiology of the future. This review looks at these new and exciting technologies.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Agulhas Current Properties Shape Microbial Community Diversity and Potential Functionality

Understanding the impact of oceanographic features on marine microbial ecosystems remains a major ecological endeavour. Here we assess microbial diversity, community structure and functional capacity along the Agulhas current system and the Subtropical convergence in the South Indian Ocean (SIO). Samples collected from the epipelagic, oxygen minimum and bathypelagic zones were analysed by 16S rRNA gene amplicon and metagenomic sequencing. In contrast to previous studies, we found high taxonomic richness in surface and deep water samples, but generally low richness for oxygen minimum zone communities. Beta-diversity analysis revealed significant dissimilarity between the three water depths. Most microbial communities were dominated by marine Gammaproteobacteria, with strikingly low levels of picocyanobacteria. Community composition was strongly influenced by specific environmental factors including depth, salinity, and the availability of both oxygen and light. Carbon, nitrogen and sulphur cycling capacity is the SIO was linked to several autotrophic and copiotrophic Alphaproteobacteria and Gammaproteobacteria. Taken together, our data suggest that the environmental conditions in the Agulhas current system, particularly depth-related parameters, substantially influence microbial community structure. While the capacity for biogeochemical cycling of nitrogen and sulphur is linked primarily by the dominant Gammaproteobacterial taxa, ecologically rare taxa drive carbon cycling.

59 BASIC BIOLOGICAL SCIENCES↗

Energy shift of Fe-K fluorescence lines due to low ionization demonstrated with XRISM in Centaurus X-3

The Fe K$\alpha$ fluorescence line at 6.4 keV is a powerful probe of cold matter surrounding X-ray sources and has been widely used in various astrophysical contexts. The X-ray microcalorimeter spectrometer onboard XRISM can measure line shifts with unprecedented precision of ${\sim }$0.2 eV, equivalent to a line-of-sight velocity of ${\sim }$10 km s$^{-1}$. At this level of accuracy, however, several factors that influence the line energy must be carefully considered prior to astrophysical interpretation. One such important factor is the ionization degree, Fe$^{q+}$. The K$\alpha$ line shifts redward by ${\sim }$4 eV as q increases from 0 (neutral) to 8 (Ar-like). Additionally, the accompanying Fe K$\beta$ line at 7.06 keV shifts blueward by ${\sim }$30 eV from $q=0$ to 8. We demonstrate that this effect is actually observable in the XRISM data of the high-mass X-ray binary Centaurus X-3 (Cen X-3). We advocate that the differential energy shift between the K$\alpha$ and K$\beta$ line provides a robust estimate of q by decoupling from other effects that shift the two lines in the same direction. We derived $q \sim 5$ (Sc-like) for the fluorescing matter by comparing the observation with atomic structure calculations of our own and in the literature. By accounting for the derived charge state and the corresponding shift in the rest-frame line energy, we made corrections for this effect and reached a consistent residual shift among the K$\alpha$, K$\beta$, and the optical measurement attributable to the systemic velocity of the system. Consequently, we obtained a new constraint on the location of the cold matter. This ionization effect needs to be assessed in all use cases of the Fe K$\alpha$ line shift beyond Cen X-3, and the proposed metric is generally applicable to all of them.

Astronomy and AstroPhysics↗

Echelle efficiencies - Theory and experiment

The effect of one echelle spectrograph design factor, orientation of the incident light bundle, is examined. Comparison of theoretical calculations and experimental measurements of echelle grating efficiencies are given for R2 echelles used in three possible configurations: alpha greater than beta, alpha smaller than beta, and the quasi-Littrow mode. The throughput-resolution products for these various cases are also compared.

Schroeder, D. J.↗

Neutronics of Advanced-Fuel Fusion Experiments with DT Triggering

Nuclear fusion studies are currently devoted to the Deuterium-Tritium (DT) fuel cycle, the easiest way to reach ignition. The recent stress on safety by the world community has stimulated the research on other fuel cycles, based on advanced reactions, such as Deuterium-Helium-3 (DHe). With DHe, it is not necessary to breed and fuel tritium. The DHe cycle has a very low presence of energetic fusion neutrons, due to DD and DT side reactions, and it has the possibility to obtain electrical power by direct energy conversion of the protons produced by the fusion reactions. DHe fusion has its own set of problems, such as the availability of {sup 3}He and the attainment of the higher plasma parameters. To explore the possibilities of DHe plasmas, a burning plasma experiment at high magnetic field and high plasma densities is particularly attractive. Ignitor is a proposed compact high-magnetic field tokamak, aimed at studying plasma-burning conditions in Deuterium-Tritium plasmas. The Ignitor experiment is designed to reach ignition under controlled DT burning conditions. The machine parameters have been established, on the basis of existing knowledge of the confinement properties of high density plasmas. The scientific goal of the Ignitor experiment is to approach, for the first time, the ignition conditions of a magnetically confined D-T plasma. However, the plasma density limit in Ignitor being well above the optimal density for DT ignition, is potentially suitable to the higher densities required for DHe burning. In fact, Ignitor has been designed also to explore conditions where 14.7-MeV protons and 3.6-MeV alpha particles produced by the DHe reactions can supply a significant amount of thermal energy to a well-confined plasma. In particular, Ignitor can sustain plasma currents exceeding those required to confine proton orbits at birth, and has more than sufficiently high densities so that the slowing-down time of both the protons and the DT alpha particles is shorter than the electron energy replacement time of the thermal plasma in which they are produced. Preliminary analyses show that a fusion power PF around 2 MW, may be reached. As a start, Ignitor can perform initial studies at the level of approximately 1 MW of power in charged particles from the DHe reaction in a mostly DT plasma. CANDOR, a design evolution of Ignitor in the direction of a reactor using a DHe fuel cycle, has been proposed; it is a feasibility study of a high-field DHe experiment of larger dimensions and higher fusion power than Ignitor, however still based on the core Ignitor technologies. The main characteristics of the CANDOR machine are the following: the major radius Ro is about double than Ignitor, plasma currents up to 25 MA with toroidal magnetic fields B{sub T} = 13 T can be produced. Unlike Ignitor, CANDOR would operate with higher values of poloidal beta around unity and the central part of the plasma column in the Second Stability region. The characteristic times over which the plasma discharge can be sustained are longer by more than a factor of 4 than those of Ignitor. To produce such a strong magnetic field in a larger machine, the toroidal field coils have been divided into two sets of coils and the central solenoid (air core transformer) is placed between them in the inboard part. In CANDOR, the DHe burning regime can be reached, by a combination of ICRF heating and alpha particle heating due to DT fusion reactions, which take the role of a sort of trigger. Thanks to this fact, and unlike other proposed DHe fusion experiments, CANDOR is capable of reaching DHe ignition using the existing technologies, and present knowledge of plasma confinement phenomena. With the initial use of DT, where tritium comprises some 50% of an initial lower density DT plasma, but is not added thereafter, the need for an intense auxiliary heating, which is one of the main technological drawbacks of DHe ignition, would be considerably alleviated, becoming feasible with the present technology. However, this method has the disadvantage of using tritium and of presenting a higher neutron flux (due to DT reactions) than 'pure' DHe plasmas, generating a neutron flux transient when passing from the initial DT trigger reaction to the final DHe burning plasma. However, for an experimental machine one-of-a-kind of relatively small dimensions, this is not an issue. (authors)

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Background reduction in the SNO+ experiment

SNO+ is a large multi-purpose liquid scintillator experiment, which first aim is to detect the neutrinoless double beta decay of {sup 130}Te. It is placed at SNOLAB, at 6000 m.w.e. and it is based on the SNO infrastructure. SNO+ will contain approximately 780 tonnes of liquid scintillator, loaded with {sup 130}Te inside an acrylic vessel (AV) with an external volume of ultra pure water to reduce the external backgrounds. Light produced in the scintillator by the interaction of particles will be detected with about 9,000 photomultiplier’s. For the neutrinoless double beta decay phase, due to its the extremely low rate expected, the control, knowledge and reduction of the background is essential. Moreover, it will also benefit other phases of the experiment focused on the study of solar neutrinos, nucleon decay, geoneutrinos and supernovae. In order to reduce the internal background level, a novel purification technique for tellurium loaded scintillators has been developed by the collaboration that reduces the U/Th concentration and several cosmic-activated isotopes by at least a factor 10{sup 2} -10{sup 3} in a single pass. In addition, different rejection techniques have been developed for the remaining internal backgrounds based on Monte-Carlo simulations. In this work, the scintillator purification technique and the levels obtained with it will be discussed. Furthermore, an overview of the different backgrounds for the double-beta phase will be presented, highlighting some of the techniques developed to reject the remained decays based on their expected timing differences.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Search for 0ν2β of {sup 100}Mo by NEMO-3 and status of SuperNEMO

The NEMO-3 experiment was devoted to the research of the neutrinoless double beta decay and the precise measurement of the two-neutrino double beta decay of seven isotopes. The detector took data in the Laboratoire Souterrain de Modane (LSM) from 2003 to 2011. The unique combination of a 3D-tracker and a calorimeter allowed to fully reconstruct the double beta decay events topology and strongly reduces the backgrounds. This feature also permitted to characterize the remaining backgrounds in independent analysis channels. With an exposure of 34.7 kg·y of {sup 100}Mo no evidence for the 0ν2β signal has been found, yielding a limit for the light Majorana neutrino mass mechanism of T{sub 1/2}(0ν2β) > 1.1 10{sup 24} years (90 % C.L.) once both statistical and systematic uncertainties are taken into account. Depending on the Nuclear Matrix Elements this corresponds to an upper limit on the Majorana effective neutrino mass of |m{sub ββ}| < 0.3 − 0.9 eV (90 % C.L.). Constraints on other lepton number violating mechanisms of 0ν2β decays are also given. Searching for high-energy double electron events in all suitable sources of the detector, no event in the energy region [3.2-10] MeV is observed for an exposure of 47 kg·y. The next generation experiment, SuperNEMO, will use the same experimental technique to extend the sensitivity on the neutrinoless double beta decay search. Several aspects have been improved compared to NEMO-3: choice of the isotope, energy resolution, energy losses… but the main challenge is the background reduction. All the detector materials have been selected for their low natural radioactivity contaminations through High Purity Germanium (HPGe) gamma spectroscopy. The double beta sources after enrichment are being prepared with extreme care and the ultimate contaminations will be characterized by the most sensitive BiPo detector. External radon tightness and internal radon emanation have been studied to ensure a very low level in the tracker with very sensitive complementary detectors: diffusion apparatus, concentration line and emanation tanks. The construction of the first detector, called the demonstrator, has started in 2012. Studying 7 kg of {sup 82}Se, the demonstrator will reach the NEMO-3 sensitivity in only 5 months and improve it by at least a factor 6 in 2.5 years. Underground installation will start fall 2014 and physics data taking should start by summer 2015.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Absence of estrogen receptor beta leads to abnormal adipogenesis during early tendon healing by an up‐regulation of PPARγ signalling

Abstract Achilles tendon injury is one of the challenges of sports medicine, the aetiology of which remains unknown. For a long time, estrogen receptor β (ERβ) has been known as a regulating factor of the metabolism in many connective tissues, such as bone, muscle and cartilage, but little is known about its role in tendon. Recent studies have implicated ERβ as involved in the process of tendon healing. Tendon‐derived stem cells (TDSCs) are getting more and more attention in tendon physiological and pathological process. In this study, we investigated how ERβ played a role in Achilles tendon healing. Achilles tendon injury model was established to analyse how ERβ affected on healing process in vivo. Cell proliferation assay, Western blots, qRT‐PCR and immunocytochemistry were performed to investigate the effect of ERβ on TDSCs. Here, we showed that ERβ deletion in mice resulted in inferior gross appearance, histological scores and, most importantly, increased accumulation of adipocytes during the early tendon healing which involved activation of peroxisome proliferator‐activated receptor γ (PPARγ) signalling. Furthermore, in vitro results of ours confirmed that the abnormity might be the result of abnormal TDSC adipogenic differentiation which could be partially reversed by the treatment of ERβ agonist LY3201. These data revealed a role of ERβ in Achilles tendon healing for the first time, thereby providing a new target for clinical treatment of Achilles tendon injury.

Bian, Xuting↗

Comparing the extraction performance of cyclodextrin-containing supramolecular deep eutectic solvents versus conventional deep eutectic solvents by headspace single drop microextraction

A headspace single drop microextraction (HS-SDME) method coupled with high performance liquid chromatography was developed to compare the extraction of eighteen aromatic organic pollutants from aqueous solutions using cyclodextrin-based supramolecular deep eutectic solvents (SUPRADESs) and alkylammonium halide-based conventional deep eutectic solvents (DESs). Different derivatives of beta-cyclodextrin (β-CD) were employed as hydrogen bond acceptors (HBA) in SUPRADESs and the extraction performance investigated. SUPRADES comprised of the 20 wt% native β-CD HBA provided the highest enrichment factors of analytes compared to SUPRADESs comprised of other derivatives of β-CD (random methylated β-cyclodextrin, heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin, and 2-hydroxypropyl β-cyclodextrin). In addition, native β-CD and its derivatives were dissolved in the neat DESs and their effect on the extraction of analytes examined. Dissolution of 20 wt% native β-CD in the choline chloride ([Ch + ][Cl - ]):2Urea DES resulted in a significant increase in the extraction efficiencies of target analytes compared to the neat [Ch + ][Cl - ]:2Urea DES. Under optimum conditions, the extraction method required a solvent microdroplet of 6.5 μL, 1000 rpm stir rate, 30% (w/v) salt concentration, and a temperature of 40 °C. The tetrabutylammonium chloride: 2 lactic acid DES resulted in the highest enrichment factors while the [Ch + ][Cl - ]:2Urea DES had the lowest for most of the analytes among the evaluated solvents. The method provided limits of detection (LODs) down to 35 μg L -1 . Finally, the developed method was applied for the analysis of spiked tap and lake water, where relative recoveries ranging from 83.7% -119.7% and relative standard deviations lower than 19.2% were achieved.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Results from KamLAND-Zen

KamLAND-Zen reports on a preliminary search for neutrinoless double-beta decay with 136 Xe based on 114.8 live-days after the purification of the xenon loaded liquid scintillator. In this data, the problematic 110m Ag background peak identified in previous searches is reduced by more than a factor of 10. By combining the KamLAND-Zen pre- and post-purification data, we obtain a preliminary lower limit on the 0νββ decay half-life of T 0ν 1/2 > 2.6×10 25 yr at 90% C.L. The search sensitivity will be enhanced with additional low background data after the purification. As a result, prospects for further improvements with future KamLAND-Zen upgrades are also presented.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

PGC-1{beta} regulates mouse carnitine-acylcarnitine translocase through estrogen-related receptor {alpha}

Highlights: Black-Right-Pointing-Pointer The Cact gene is induced in mouse skeletal muscle after 24 h of fasting. Black-Right-Pointing-Pointer The Cact gene contains a functional consensus sequence for ERR. Black-Right-Pointing-Pointer This sequence binds ERR{alpha} both in vivo and in vitro. Black-Right-Pointing-Pointer This ERRE is required for the activation of Cact expression by the PGC-1/ERR axis. Black-Right-Pointing-Pointer Our results add Cact as a genuine gene target of these transcriptional regulators. -- Abstract: Carnitine/acylcarnitine translocase (CACT) is a mitochondrial-membrane carrier proteins that mediates the transport of acylcarnitines into the mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway. CACT deficiency causes a variety of pathological conditions, such as hypoketotic hypoglycemia, cardiac arrest, hepatomegaly, hepatic dysfunction and muscle weakness, and it can be fatal in newborns and infants. Here we report that expression of the Cact gene is induced in mouse skeletal muscle after 24 h of fasting. To gain insight into the control of Cact gene expression, we examine the transcriptional regulation of the mouse Cact gene. We show that the 5 Prime -flanking region of this gene is transcriptionally active and contains a consensus sequence for the estrogen-related receptor (ERR), a member of the nuclear receptor family of transcription factors. This sequence binds ERR{alpha}in vivo and in vitro and is required for the activation of Cact expression by the peroxisome proliferator-activated receptor gamma coactivator (PGC)-1/ERR axis. We also demonstrate that XTC790, the inverse agonist of ERR{alpha}, specifically blocks Cact activation by PGC-1{beta} in C2C12 cells.

60 APPLIED LIFE SCIENCES↗

Occultation of a compact radio source by Venus

An occultation of the compact radio source P 0507+17 by Venus on 19 Jul. 1988, was observed in Tidbinbilla, Australia at a frequency of 2.3 GHz. The purpose of this observation was to measure the position of Venus in the radio reference frame. When data from both ingress (Venus dayside) and egress (Venus nightside) were used to solve for the position of Venus in ecliptic longitude and latitude, the results were consistent with zero offsets from the nominal values, with an uncertainty of approximately 0.2 arcsec in both coordinates. By using the nightside data alone, a value of -0.026 +/- 0.04 arcsec was obtained for the linear combination delta(lambda) + 0.51delta(beta), where delta(lambda) and delta(beta) were the offsets from their nominal values of the ecliptic longitude and latitude of Venus. Distortion of a vacuum Fresnel fringe pattern by the Venus troposphere, and especially by the Venus ionosphere, was observed. The dayside ionosphere of Venus caused very large distortions; the amplitude of the first Fresnel fringe in the ingress data was eight times larger than was expected for an airless planet. The observed fringe patterns were modeled by using plausible ionospheres (i.e., consistent with spacecraft measurements of the Venus ionosphere and with solar extreme ultraviolet flux and solar wind pressure measurements at the occultation epoch). However, the range of Venus ionospheric profiles (electron density as a function of altitude) allowed by a priori constraints and by the occultation data was large (e.g., the ionopause height on the dayside was uncertain by a factor of two). This ionospheric uncertainty (particularly on the dayside) translated into a large position uncertainty (0.2 arcsec for the dayside and 0.04 arcsec for the nightside). If it was possible to calibrate the Venus ionosphere by some external means, the accuracy in delta(lambda) and delta(beta) would have been 0.01 arcsec or better.

Linfield, R.↗

SU-C-201-06: Utility of Quantitative 3D SPECT/CT Imaging in Patient Specific Internal Dosimetry of 153-Samarium with GATE Monte Carlo Package

Purpose: Patient-specific 3-dimensional (3D) internal dosimetry in targeted radionuclide therapy is essential for efficient treatment. Two major steps to achieve reliable results are: 1) generating quantitative 3D images of radionuclide distribution and attenuation coefficients and 2) using a reliable method for dose calculation based on activity and attenuation map. In this research, internal dosimetry for 153-Samarium (153-Sm) was done by SPECT-CT images coupled GATE Monte Carlo package for internal dosimetry. Methods: A 50 years old woman with bone metastases from breast cancer was prescribed 153-Sm treatment (Gamma: 103keV and beta: 0.81MeV). A SPECT/CT scan was performed with the Siemens Simbia-T scanner. SPECT and CT images were registered using default registration software. SPECT quantification was achieved by compensating for all image degrading factors including body attenuation, Compton scattering and collimator-detector response (CDR). Triple energy window method was used to estimate and eliminate the scattered photons. Iterative ordered-subsets expectation maximization (OSEM) with correction for attenuation and distance-dependent CDR was used for image reconstruction. Bilinear energy mapping is used to convert Hounsfield units in CT image to attenuation map. Organ borders were defined by the itk-SNAP toolkit segmentation on CT image. GATE was then used for internal dose calculation. The Specific Absorbed Fractions (SAFs) and S-values were reported as MIRD schema. Results: The results showed that the largest SAFs and S-values are in osseous organs as expected. S-value for lung is the highest after spine that can be important in 153-Sm therapy. Conclusion: We presented the utility of SPECT-CT images and Monte Carlo for patient-specific dosimetry as a reliable and accurate method. It has several advantages over template-based methods or simplified dose estimation methods. With advent of high speed computers, Monte Carlo can be used for treatment planning on a day to day basis.

60 APPLIED LIFE SCIENCES↗

Regulation of the pro-inflammatory cytokine osteopontin by GIP in adipocytes - A role for the transcription factor NFAT and phosphodiesterase 3B

Highlights: Black-Right-Pointing-Pointer GIP stimulates lipogenesis and osteopontin expression in primary adipocytes. Black-Right-Pointing-Pointer GIP-induced osteopontin expression is NFAT-dependent. Black-Right-Pointing-Pointer Osteopontin expression is PDE3-dependent. Black-Right-Pointing-Pointer Osteopontin expression is increased in PDE3B KO mice. -- Abstract: The incretin - glucose-dependent insulinotropic polypeptide (GIP) - and the pro-inflammatory cytokine osteopontin are known to have important roles in the regulation of adipose tissue functions. In this work we show that GIP stimulates lipogenesis and osteopontin expression in primary adipocytes. The GIP-induced increase in osteopontin expression was inhibited by the NFAT (the transcription factor nuclear factor of activated T-cells) inhibitor A-285222. Also, the NFAT kinase glycogen synthase kinase (GSK) 3 was upregulated by GIP. To test whether cAMP might be involved in GIP-mediated effects on osteopontin a number of strategies were used. Thus, the {beta}3-adrenergic receptor agonist CL316,243 stimulated osteopontin expression, an effects which was mimicked by OPC3911, a specific inhibitor of phosphodiesterase 3. Furthermore, treatment of phosphodiesterase 3B knock-out mice with CL316,243 resulted in a dramatic upregulation of osteopontin in adipose tissue which was not the case in wild-type mice. In summary, we delineate mechanisms by which GIP stimulates osteopontin in adipocytes. Given the established link between osteopontin and insulin resistance, our data suggest that GIP by stimulating osteopontin expression, also could promote insulin resistance in adipocytes.

60 APPLIED LIFE SCIENCES↗