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At least 541 records · Page 30

Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MS

The work described herein assesses the ability to characterize gold nanoparticles (Au NPs) of 50 and 100 nm, as well as 60 nm silver shelled gold core nanospheres (Au/Ag NPs), for their mass, respective size, and isotopic composition in an automated and unattended fashion. Here, an innovative autosampler was employed to mix and transport the blanks, standards, and samples into a high-efficiency single particle (SP) introduction system for subsequent analysis by inductively coupled plasma–time of flight–mass spectrometry (ICP-TOF-MS). Optimized NP transport efficiency into the ICP-TOF-MS was determined to be >80%. This combination, SP-ICP-TOF-MS, allowed for high-throughput sample analysis. Specifically, 50 total samples (including blanks/standards) were analyzed over 8 h, to provide an accurate characterization of the NPs. This methodology was implemented over the course of 5 days to assess its long-term reproducibility. Impressively, the in-run and day-to-day variation of sample transport is assessed to be 3.54 and 9.52% relative standard deviation (%RSD), respectively. The determination of Au NP size and concentration was of <5% relative difference from the certified values over these time periods. Isotopic characterization of the 107 Ag/ 109 Ag particles (n = 132,630) over the course of the measurements was determined to be 1.0788 ± 0.0030 with high accuracy (0.23% relative difference) when compared to the multi-collector–ICP-MS determination.

36 MATERIALS SCIENCE↗

Comparison of the Transcriptome Response within the Swine Tracheobronchial Lymphnode Following Infection with PRRSV, PCV-2 or IAV-S.

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major respiratory pathogen of swine that has become extremely costly to the swine industry worldwide, often causing losses in production and animal life due to their ease of spread. However, the intracellular changes that occur in pigs following viral respiratory infections are still scantily understood for PRRSV, as well as other viral respiratory infections. The aim of this study was to acquire a better understanding of the PRRS disease by comparing gene expression changes that occur in tracheobronchial lymph nodes (TBLN) of pigs infected with either porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV-2), or swine influenza A virus (IAV-S) infections. The study identified and compared gene expression changes in the TBLN of 80 pigs following infection by PRRSV, PCV-2, IAV-S, or sham inoculation. Total RNA was pooled for each group and time-point (1, 3, 6, and 14 dpi) to make 16 libraries—analyses are by Digital Gene Expression Tag Profiling (DGETP). The data underwent standard filtering to generate a list of sequence tag raw counts that were then analyzed using multidimensional and differential expression statistical tests. The results showed that PRRSV, IAV-S and PCV-2 viral infections followed a clinical course in the pigs typical of experimental infection of young pigs with these viruses. Gene expression results echoed this course, as well as uncovered genes related to intersecting and unique host immune responses to the three viruses. By testing and observing the host response to other respiratory viruses, our study has elucidated similarities and differences that can assist in the development of vaccines and therapeutics that shorten or prevent a chronic PRRSV infection.

Miller↗

Using Gamification to Enhance Mastery of Network Security Concepts

Gamification has proven to be effective in engaging and encouraging people to work towards and achieve goals. Many students struggle to focus on schoolwork, due to a lack of interest, lack of understanding, or other factors unique to the student. Applying gamification elements to education can help engage these students in learning their course material and help them excel academically. This study examines the effectiveness of using gamification techniques to enhance the learning experience in college Computer Science courses. A video game application is utilized to review and reinforce cybersecurity concepts that students have already been taught in class. Previous work has been made on a prototype game build that teaches about ARP (Address Resolution Protocol) components. The focus of this study is to refine and develop the structure of the prototype into a more interactive and enjoyable format with non-competitive and captivating activities that allow students to study at their own pace. An updated version of the game was created that focused on reaching a balance between education and entertainment. The game was used by students enrolled in a cybersecurity class, where pre-survey, post-survey and a focus group interview were conducted to determine how effective the updated version is compared to the current build, in addition to how effective the gamification method is regarding student retention of taught material. The pre-survey and post-survey results revealed an increase in interest and mastery of cybersecurity concepts as a result of playing the game. Students found value in the game as both a method of reviewing material taught in class and an entertaining and engaging game. These results show potential in using gamification in cybersecurity and education.

Hilliard, Kevin↗

Dyna-Soar Bioastronautics

Dyna-Soar is a manned boost-orbit-glide vehicle. Conventional booster and guidance systems will place the glider on a course at the proper altitude and velocity with the resultant energy to accomplish the planned mission. After boost termination, the pilot verifies the course during orbit and controls the flight path during reentry, approach, and landing. The flight profiles and over-all flight regime for the Dyna-Soar's single-orbit mission will impose no new or extreme environmental hazards on the pilot. '!he environmental parameters of a normal space flight will not expose the pilot to stress magnitudes as great as pilots have experienced in the Mercury flights. Under presently planned flights, the pilot should be in an environment that is well within known human tolerances. We will present the calculated environmental stress to be imposed on the pilot in both normal and emergency conditions and the design techniques .developed to provide a cockpit that will keep the pilot well within his tolerance limits. The various primary environmental parameters will be discussed separately. The solution for each parameter will be discussed in a concurrent manner. No significance is attached to the order in which the specific parameters of the environment are considered.

Spacecraft↗

Rendezvous Guidance Technology

The literature on rendezvous guidance technology is reviewed and a bibliography of about 160 documents is presented. The importance of the proper selection of the ascent trajectory and of the orbit for a manned space station or an orbiting launch complex is emphasized. The way the launch delay problem affects the selection of the ascent trajectory and the target orbit is discussed. A specific ascent trajectory and an orbit for a manned space station or an orbiting launch complex is recommended on the premise that launch delay problems will continue to outweigh most of the other orbit selection criteria. A new method for determining the velocity corrections required for mid-course guidance is proposed . The proposed methods consist of an "exact-numerical" solution of the relative equations of motion in the "Shell-coordinate system." The proposed "exact-numerical" method extends the applicability of the mid- course equations to a much greater range than that of the currently available linear methods .

Trajectory↗

System safety education focused on flight safety

The measures necessary for achieving higher levels of system safety are analyzed with an eye toward maintaining the combat capability of the Air Force. Several education courses were provided for personnel involved in safety management. Data include: (1) Flight Safety Officer Course, (2) Advanced Safety Program Management, (3) Fundamentals of System Safety, and (4) Quantitative Methods of Safety Analysis.

Eugene Holt↗

High Pressure Reverse Flow APS Engine

A design and test demonstration effort was undertaken to evaluate the concept of the reverse flow engine for the APS engine application. The 1500 lb (6672 N) thrust engine was designed to operate on gaseous hydrogen and gaseous oxygen propellants at a mixture ratio of 4 and to achieve the objective performance of 435 sec (4266 Nsec/kg) specific impulse. Superimposed durability requirements called for a million-cycle capability with 50 hours duration. The program was undertaken as a series of tasks including the initial preliminary design, design of critical test components and finally, the design and demonstration of an altitude engine which could be used interchangeably to examine operating parameters as well as to demonstrate the capability of the concept. The program results are reported with data to indicate that all of the program objectives were met or exceeded within the course of testing on the program. The analysis effort undertaken is also reported in detail and supplemented with test data in some cases where prior definitions could not be made. The results are contained of these analyses as well as the test results conducted throughout the course of the program. Finally, the test data and analytical results were combined to allow recommendations for a flight weight design. This preliminary design effort is also detailed.

Senneff, J. M.↗

ERTS-A data as a teaching and research tool in the Department of Geology

The author has identified the following significant results. ERTS-1 prints have been used extensively in a geology of Alaska class to give a basic framework of the geology of the state. In addition, they have been intermittantly used in such diverse classes as: (1) Economic Geology (e.g. the Sn-bearing granites of the Seward Peninsula are particularly noticeable due to their wide contact metamorphic aureoles.) (2) A canned geology of Alaska lecture which has been given to two different introductory geology courses. (3) Structural Geology (e.g. the Fairweather and Denali faults are striking obvious). It was found most convenient for larger classes to prepare 35mm slides of the ERTS-1 prints that are used in conjunction with slides of the topographic and geologic maps at about the same scale. Thus the emphasis has been in integration of the ERTS-1 material into existing courses. As such, the ERTS-1 data has provided a unique and striking viewpoint that never fails to initiate favorable comment. In addition, prints have been examined by numerous researchers to develop a regional, integrated overview of such varied topics as regional geology to a background for local geologic mapping to studies of ore deposits and to the definition of a formation to be studied in detail at its type locality.

Grybeck, D.↗

ERTS-1 evaluation of natural resources management applications in the Great Basin

The relatively cloud free weather in the Great Basin has allowed the accumulation of several dates of excellent ERTS-1 imagery. Mountains, valleys, playas, stream courses, canyons, alluvial fans, and other landforms are readily delineated on ERTS-1 imagery, particularly with MSS-5. Each band is useful for identifying and studying one or more natural resource features. For example, crested wheatgrass seedings were most easily identified and measured on MSS-7. Color enhancements simulating CIR were useful for depicting meadow and phreatophytic vegetation along water bodies and stream courses. Work is underway to inventory and monitor wildfire areas by age and successional status. Inventories have been completed on crested wheatgrass seedings over the entire State of Nevada, and inventories of playa surfaces, water surfaces, phreatophytic vegetation, snow cover, meadows, and other features is continuing. Vegetation ecotones are being delineated for vegetation mapping. The pinyon/juniper-northern desert shrub ecotone has been identified with considerable success. Phenology changes can be used to describe vegetation changes for management.

Tueller, P. T.↗

Study of recreational land and open space using Skylab imagery

The author has identified the following significant results. Sl90B color photography has adequate resolution to detect or identify many natural and cultural features which are significant for the evaluation of recreational land and open space. The effectiveness of the interpretation could be improved if coverage were obtained at two or three different seasons so that indicators such as seasonal changes in vegetation cover or snow enhancement could aid the interpretation of land use and land cover. The degree of detail which can be observed from Sl90B photography makes it useful for the following purposes: (1) Photography can be used to obtain general familiarity with a large regional area, and to study interrelationships of major natural and cultural features within the area. (2) Existing recreational facilities such as golf courses, parks, stadiums, race tracks, and marinas can be detected and identified. (3) The photography can be used for initial selection of recreation sites. (4) It would also indicate trends in population growth, which constitute one type of information needed in estimating the growth of recreation demand. The photography has only limited use for planning individual site planning of parks, golf courses, and other recreation facilities.

Sattinger, I. J.↗

Computer-assisted instruction

The results are presented of a project of research and development on strategies for optimizing the instructional process, and dissemination of information about the applications of such research to the instructional medium of computer-assisted instruction. Accomplishments reported include construction of the author language INSTRUCT, construction of a practical CAI course in the area of computer science, and a number of investigations into the individualization of instruction, using the course as a vehicle.

Atkinson, R. C.↗

ERTS-A data as a teaching and research tool in the Department of Geology

The author has identified the following significant results. The project was an attempt to integrate ERTS-1 data into teaching introductory, specialized, and graduate courses in the Department of Geology, University of Alaska. This data was to be utilized principally through a specially selected, high quality collection of black and white, and color 9.5 mosaics of the State of Alaska. In completing these tasks, the data accumulated has proved highly useful in a variety of ways including: (1) discussions of the uses and availability of ERTS imagery; (2) as a medium for talking about and showing various areas of Alaska; (3) in discussing geology in general; and (4) as an aid in doing research and as possible research topics themselves. Use of ERTS-1 imagery in geology proved highly successful and its use is now an integral part of many courses.

Grybeck, D.↗

Interactive analysis and evaluation of ERTS data for regional planning and urban development: A Los Angeles Basin case study

The progression endemic to the ERTS Data Use Experiment SR 124 in data quality, analysis sophistication and applications responsiveness is reviewed. The roles of the variety of ERTS products, including the supporting underflight aircraft imagery at various scales, are discussed in the context of this investigation. The versatility of interpretation techniques and outputs developed and implemented via the General Electric Multispectral Information Extraction Systems is described and exemplified by both system-expository and applications-explanatory products. The wide-ranging and in-depth applications studied in the course of this experiment can be characterized as community-oriented and agency-directed. In the former, generic category, which is primarily data-contextual, problems analyzed dealt with agricultural systems, surface water bodies, snow cover, brush fire burns, forestry, grass growth, parks - golf courses - cemeteries, dust storms, grading sites, geological features and coastal water structure. The ERTS MSS band selectivity and measurements thresholds were of primary interest here. The agency-directed application areas have been user-evaluational in nature. Beginning with overall urbanized regional analysis of land cover density-development intensity, residential areas were analyzed for ascertaining if housing types could be aggregated with any degree of reliability.

Raje, S.↗

Notes on Space Technology

These notes are part of a space technology course given at the Flight Research Division of the NACA Langley Aeronautical Laboratory during the early part of 1958. The course was conducted as a self-education program within the Flight Research Division and the various Sections of the notes were prepared for the most part by members of the Division; however, four of the seventeen Sections were prepared by personnel from the Pilotless Aircraft Research Division and the Compressibility Research Division who were very helpful in making the program more complete. The notes have been compiled on a brief time schedule and it will be apparent to the reader that the present version is incomplete and to some extent may lack uniformity in length, type of presentation, and technical detail in the various Sections. Nevertheless, there has been a demand for the notes from those who have seen them, and it is thought that they might serve a useful purpose if they were made available on a wider basis. It is believed that for the sake of expediency this goal is best achieved by making the material available now in its present unedited form instead of following the usual NACA editing procedures.

Henry A Pearson↗

The ME/DE degree at the University of Kansas

An effort to provide advanced engineering education for aeronautical engineers is discussed. The Doctor of Engineering program involves a technical education in the area of interest above that of the M.S. degree. In addition, there is a core of systems, design, business, and technology and society courses which all students must take. Twenty-four hours are required in the core area. The original courses as modified with time still exist. A recent review has indicated a need for change. The core will remain, but it is the hope that the student will have a selection of 30 hours in these areas from which to pick 24 hours. The areas will be changed such that system analyses and design will be integrated to a further extent. The business area will be expanded. Areas such as technology assessment and forecasting will be added.

Lenzen, K. H.↗

College curriculum-sharing via CTS

Domestic communication satellites and video compression techniques will increase communication channel capacity and reduce cost of video transmission. NASA Ames Research Center, Stanford University and Carleton University are participants in an experiment to develop, demonstrate, and evaluate college course sharing techniques via satellite using video compression. The universities will exchange televised seminar and lecture courses via CTS. The experiment features real-time video compression with channel coding and quadra-phase modulation for reducing transmission bandwidth and power requirements. Evaluation plans and preliminary results of Carleton surveys on student attitudes to televised teaching are presented. Policy implications for the U.S. and Canada are outlined.

Hudson, H. E.↗

Chemistry of vaporization of refractory materials

A discussion is given of the principles of physical chemistry important in vaporization studies, notably the concepts of equilibrium, phase behavior, thermodynamics, solid solution, and kinetics. The important factors influencing equilibrium vaporization phenomena are discussed and illustrated. A proper course of a vaporization study consisting of 9 stages is proposed. The important experimental techniques of Knudsen effusion, Langmuir vaporization and mass spectrometry are discussed. The principles, the factors, the course of a study and the experimental techniques and procedures are illustrated by recent work on the Ti-O system.

Gilles, P. W.↗

Formation of iron sulphide in solar nebula

Noting that the iron sulfide in the Orgueil carbonaceous meteorite is an Fe-deficient monosulfide (pyrrhotite), it is suggested that such mineral chemistry is inconsistent with equilibrium condensation of the solar nebula and that the course of condensation may have been modified by kinetic effects. The effect of Ni on the reaction between Fe and S to produce FeS is examined, and possible reasons are considered for the fact that the cited meteorite differs in both crystal structure and Ni content from the predictions of equilibrium condensation. It is proposed that sulfide formation in the solar nebula may have been inhibited by sluggish diffusion, so that sulfur began to react with previously condensed troilite to form pyrrhotite. On this basis, observations of the Orgueil sulfides are shown to suggest that the course of solar-system condensation was modified by kinetic effects below about 700 K and that equilibrium may not have been achieved.

Kerridge, J. F.↗