Search NASA⌕ Search

SEARCH · Search NASA

Results for “Decay processes”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 559 records · Page 31

Design of a Multi-Level/Analog Ferroelectric Memory Device

Increasing the memory density and utilizing the dove1 characteristics of ferroelectric devices is important in making ferroelectric memory devices more desirable to the consumer. This paper describes a design that allows multiple levels to be stored in a ferroelectric based memory cell. It can be used to store multiple bits or analog values in a high speed nonvolatile memory. The design utilizes the hysteresis characteristic of ferroelectric transistors to store an analog value in the memory cell. The design also compensates for the decay of the polarization of the ferroelectric material over time. This is done by utilizing a pair of ferroelectric transistors to store the data. One transistor is used as a reference to determine the amount of decay that has occurred since the pair was programmed. The second transistor stores the analog value as a polarization value between zero and saturated. The design allows digital data to be stored as multiple bits in each memory cell. The number of bits per cell that can be stored will vary with the decay rate of the ferroelectric transistors and the repeatability of polarization between transistors. It is predicted that each memory cell may be able to store 8 bits or more. The design is based on data taken from actual ferroelectric transistors. Although the circuit has not been fabricated, a prototype circuit is now under construction. The design of this circuit is different than multi-level FLASH or silicon transistor circuits. The differences between these types of circuits are described in this paper. This memory design will be useful because it allows higher memory density, compensates for the environmental and ferroelectric aging processes, allows analog values to be directly stored in memory, compensates for the thermal and radiation environments associated with space operations, and relies only on existing technologies.

MacLeod, Todd C.↗

Growth Rate of Deep Convective System Cloud Shields: Satellite Observations and Km-Scale Radiative Convective Equilibrium Simulations

Deep convection gives rise to large upper level clouds that strongly interact with radiation and are important to the climate energy budget. From an object-oriented perspective, these individual deep cloud systems are characterized by a well depicted cloud shield life cycle, starting with small cloud extents that grow at varying rates before decaying and vanishing. A simple formulation of the growth rate of the cloud shield has been proposed that links together the growth rate on the convective part of the cloud, the mass flux of both the convective and stratiform parts of the cluster and a simple removal sink term (Elsaesser et al., 2022). In this presentation we first show using a suite of satellite observations (infrared from geostationary satellites, GPM radar, etc.) that the functional form of the proposed equation is well suited to quantify the shield growth rate. We then focus on RCE simulations, with deep cloud system objects post processed, to explore the relative role of each term of the growth rate budget. Three different models are used in the same RCEMIP-like configurations. The results show that the budget equation works very well for each model, although the time constants require model-dependent adjustments. We will further show in Vienna the commonalities and the specificities of each model.

Deep convection↗

A study of the excitation and radiative decay of the 3s-prime 3D-0 and 3d 3D-0 levels of atomic oxygen

The absolute cross-sections for the excitation of the 989 A, 1027 A, 7990 A, 8446 A, 1.1287 micron and 1.3164 micron multiplets of atomic oxygen by electron impact dissociation of O2 are reported. The radiative branching ratios for these transitions are calculated from these results and compared with the NBS compilation of Wiese et al. (1966) and the recent theoretical calculations of Pradhan and Saraph (1977). The cascade models of O(+) radiative recombination and of electron-impact excitation of the O I(3S) state in the terrestrial airglow are discussed in the light of the laboratory measurements, and the effects of the resonant absorption of components of the lambda 989 A and lambda 1027 A multiplets by the Birge-Hopfield band system of N2 are investigated. This process is shown to depend sensitively on the N2 vibrational temperature and to cause characteristic changes in the OI EUV emission spectrum in auroras and in the sunlit F-region at high exospheric temperatures.

Zipf, E. C.↗

Excited state dynamics of thulium ions in yttrium aluminum garnets

The processes that take place in the excited states of a trivalent Thulium (Tm) ion in an Yttrium Aluminum Garnet (YAG) crystal, being relevant to the use of this system for laser applications, have been the object of several studies. We have reexamined this system focusing our attention on the dynamics of Tm following its excitation in the H-3(sub 4) level. Under these conditions the system relaxes through a cross-relaxation process. H-3(sub 4) yields F-3(sub 4), H-3(sub 6) yields F-3(sub 4), whose rate depends upon both the concentration of the Tm ion and the temperature of the crystal. The excitation spectrum obtained by monitoring the 1.8 micron emission of Tm (due to the F-3(sub 4) yields H-3(sub 6) transition) indicates an increase in the contribution to this emission from the H-3(sub 4) level relative to the H-3(sub 5) level as the Tm concentration increases; this shows the increased role played by the H-3(sub 4) level in pumping the infrared emission. Correspondingly, the duration of the luminescence originating in the H-3(sub 4) level is shortened as the concentration of Tm increases. The concentration quenching of this lifetime can be fit to a model which assumes that the cross-relaxation is due to a dipole-dipole interaction; from this fit, the intrinsic Tm lifetime in the absence of cross relaxation can be derived. We have used this lifetime to calculate the rate of the cross-relaxation process. We have evaluated this rate as a function of the temperature and found it to be fastest at 77 K. We have also calculated the microscopic interaction parameters for the cross-relaxation process by using two independent experimental features: (1) the time evolution of the emission from the H-3(sub 4) level; and (2) the spectral overlap between the H-3(sub 4) yields F-3(sub 4) emission and the H-3(sub 6) yields F-3(sub 4) absorption. We have also considered the migration of excitation among the Tm ions in the F-3(sub 4) level and calculated the relevant microparameter by the use of the relevant spectral overlap. The data are consistent with the model in which the Tm ions, once excited into the H-3(sub 4) level decay by cross-relaxation to the F-3(sub 4), and then transfer rapidly their energy to other Tm ions.

Armagan, G.↗

Implementation of Leak Test Methods for the International Space Station (ISS) Elements, Systems and Components

The International Space Station (ISS has Qualification and Acceptance Environmental Test Requirements document, SSP 41172 that includes many environmental tests such as Thermal vacuum & Cycling, Depress/Repress, Sinusoidal, Random, and Acoustic Vibration, Pyro Shock, Acceleration, Humidity, Pressure, Electromatic Interference (EMI)/Electromagnetic Compatibility (EMCO), etc. This document also includes (13) leak test methods for Pressure Integrity Verification of the ISS Elements, Systems, and Components. These leak test methods are well known, however, the test procedure for specific leak test method shall be written and implemented paying attention to the important procedural steps/details that, if omitted or deviated, could impact the quality of the final product and affect the crew safety. Such procedural steps/details for different methods include, but not limited to: - Sequence of testing, f or example, pressurization and submersion steps for Method I (Immersion); - Stabilization of the mass spectrometer leak detector outputs fo r Method II (vacuum Chamber or Bell jar); - Proper data processing an d taking a conservative approach while making predictions for on-orbit leakage rate for Method III(Pressure Change); - Proper Calibration o f the mass spectrometer leak detector for all the tracer gas (mostly Helium) Methods such as Method V (Detector Probe), Method VI (Hood), Method VII (Tracer Probe), Method VIII(Accumulation); - Usage of visibl ility aides for Method I (Immersion), Method IV (Chemical Indicator), Method XII (Foam/Liquid Application), and Method XIII (Hydrostatic/Visual Inspection); While some methods could be used for the total leaka ge (either internal-to-external or external-to-internal) rate requirement verification (Vacuum Chamber, Pressure Decay, Hood, Accumulation), other methods shall be used only as a pass/fail test for individual joints (e.g., welds, fittings, and plugs) or for troubleshooting purposes (Chemical Indicator, Detector Probe, Tracer Probe, Local Vacuum Chamber, Foam/Liquid Application, and Hydrostatic/Visual Inspection). Any isolation of SSP 41172 requirements have led to either retesting of hardware or accepting a risk associated with the potential system or component pressure integrity problem during flight.

Underwood, Steve↗

A Soft X-Ray Spectral Episode for the Clocked Burster, GS 1826-24 as Measured by Swift and NuSTAR

We report on NuSTAR and Swift observations of a soft state of the neutron star low-mass X-ray binary GS 1826-24, commonly known as the "clocked" burster. The transition to the soft state was recorded in 2014 June through an increase of the 2-20 keV source intensity measured by MAXI, simultaneous with a decrease of the 15-50 keV intensity measured by Swift/BAT. The episode lasted approximately two months, after which the source returned to its usual hard state. We analyze the broadband spectrum measured by Swift/XRT and NuSTAR and estimate the accretion rate during the soft episode to be approximately equal to 13% m(sub Edd), within the range of previous observations. However, the best-fit spectral model, adopting the double Comptonization used previously, exhibits significantly softer components. We detect seven type-I X-ray bursts, all significantly weaker (and with shorter rise and decay times) than observed previously. The burst profiles and recurrence times vary significantly, ruling out the regular bursts that are typical for this source. One burst exhibited photospheric radius expansionand we estimate the source distance as (5.7 +/- 0.2) xi(sub b)(exp -1/2) kpc, where xi(sub b) parameterizes the possible anisotropy of the burst emission. The observed soft state may most likely be interpreted as a change in accretion geometry at about similar bolometric luminosity as in the hard state. The different burst behavior can therefore be attributed to this change in accretion flow geometry, but the fundamental cause and process for this effect remain unclear.

accretion↗

The International Space Station (ISS) Port 1 (P1) External Active Thermal Control System (EATCS) Ammonia Leak

From 2011 to 2017, the crew onboard the International Space Station (ISS) was at risk of dire consequences due to an external ammonia leak. Ammonia is used in the External Active Thermal Control System (EATCS) to cool the pressurized modules and external electrical systems. Engineers at NASA's Johnson Space Center (JSC) initially detected the leak in one of two cooling loops by monitoring the system ammonia inventory decay over time. White flakes seen on High Definition (HD) cameras were also thought to be associated with the leakage but not confirmed. Initially, the leak was small enough that the ammonia inventory and system operations were not in jeopardy. However, the leak began to accelerate to the point where troubleshooting and corrective action were vital to the sustainability of the ISS. Therefore, it became imperative that the leak be located and repaired for ISS operations to continue. No tools were readily available on the ISS to locate such a leak when it was initially detected, however NASA engineers were already in the process of developing a new device for this purpose called the Robotic External Leak Locator (RELL). The RELL is a robotic instrument package with a mass spectrometer and an ion pressure gauge. Initial checkout operations with RELL happened to coincide with the increasing leak, and ammonia vapors were measured around the P1 EATCS Radiator #3 flexible jumper hoses. The leak stopped after the radiator and its flexible hoses were remotely isolated from the loop and the ammonia from the isolated segment was vented to space. Astronauts conducted a spacewalk that successfully removed the hoses, which were returned to ground for further investigation. The purpose of this paper is to review the leak detection and isolation efforts, investigation results, lessons learned and the recovery plan.

Cowan, Darnell T.↗

The International Space Station (ISS) Port 1 (P1) External Active Thermal Control System (EATCS) Ammonia Leak

From 2011 to 2017, the crew onboard the International Space Station (ISS) was at risk of dire consequences due to an external ammonia leak. Ammonia is used in the External Active Thermal Control System (EATCS) to cool the pressurized modules and external electrical systems. Engineers at NASA's Johnson Space Center (JSC) initially detected the leak in one of two cooling loops by monitoring the system ammonia inventory decay over time. White flakes seen on High Definition (HD) cameras were also thought to be associated with the leakage but not confirmed. Initially, the leak was small enough that the ammonia inventory and system operations were not in jeopardy. However, the leak began to accelerate to the point where troubleshooting and corrective action were vital to the sustainability of the ISS. Therefore, it became imperative that the leak be located and repaired for ISS operations to continue. No tools were readily available on the ISS to locate such a leak when it was initially detected, however NASA engineers were already in the process of developing a new device for this purpose called the Robotic External Leak Locator (RELL). The RELL is a robotic instrument package with a mass spectrometer and an ion pressure gauge. Initial checkout operations with RELL happened to coincide with the increasing leak, and ammonia vapors were measured around the P1 EATCS Radiator #3 flexible jumper hoses. The leak stopped after the radiator and its flexible hoses were remotely isolated from the loop and the ammonia from the isolated segment was vented to space. Astronauts conducted a spacewalk that successfully removed the hoses, which were returned to ground for further investigation. The purpose of this paper is to review the leak detection and isolation efforts, investigation results, lessons learned and the recovery plan.

Cowan, Darnell T.↗

The numerical design of a spherical baroclinic experiment for Spacelab flights

The near-zero G environment of Spacelab is the basis of a true spherical experimental model of synoptic scale baroclinic atmospheric processes, using a radial dielectric body force analogous to gravity over a volume of liquid within two concentric spheres. The baroclinic motions are generated by corotating the spheres and imposing thermal boundary conditions, such that the liquid is subjected to a stable radial gradient and a latitudinal gradient. Owing to mathematical difficulties associated with the spherical geometry, quantitative design criteria can be acquired only by means of numerical models. The procedure adopted required the development of two computer codes based on the Navier-Stokes equations. The codes, of which the first calculates axisymmetric steady flow solutions and the second determines the growth or decay rates of linear wave perturbations with different wave numbers, are combined to generate marginal stability curves.

Fowlis, W. W.↗

Fiber Bragg Grating Sensor System for Monitoring Smart Composite Aerospace Structures

Lightweight, electromagnetic interference (EMI) immune, fiber-optic, sensor- based structural health monitoring (SHM) will play an increasing role in aerospace structures ranging from aircraft wings to jet engine vanes. Fiber Bragg Grating (FBG) sensors for SHM include advanced signal processing, system and damage identification, and location and quantification algorithms. Potentially, the solution could be developed into an autonomous onboard system to inspect and perform non-destructive evaluation and SHM. A novel method has been developed to massively multiplex FBG sensors, supported by a parallel processing interrogator, which enables high sampling rates combined with highly distributed sensing (up to 96 sensors per system). The interrogation system comprises several subsystems. A broadband optical source subsystem (BOSS) and routing and interface module (RIM) send light from the interrogation system to a composite embedded FBG sensor matrix, which returns measurand-dependent wavelengths back to the interrogation system for measurement with subpicometer resolution. In particular, the returned wavelengths are channeled by the RIM to a photonic signal processing subsystem based on powerful optical chips, then passed through an optoelectronic interface to an analog post-detection electronics subsystem, digital post-detection electronics subsystem, and finally via a data interface to a computer. A range of composite structures has been fabricated with FBGs embedded. Stress tensile, bending, and dynamic strain tests were performed. The experimental work proved that the FBG sensors have a good level of accuracy in measuring the static response of the tested composite coupons (down to submicrostrain levels), the capability to detect and monitor dynamic loads, and the ability to detect defects in composites by a variety of methods including monitoring the decay time under different dynamic loading conditions. In addition to quasi-static and dynamic load monitoring, the system can capture acoustic emission events that can be a prelude to structural failure, as well as piezoactuator-induced ultrasonic Lamb-waves-based techniques as a basis for damage detection.

Moslehi, Behzad↗

Accuracy enhancements for overset grids using a defect correction approach

A defect-correction approach is investigated as a means of enhancing the accuracy of flow computations on overset grids. Typically, overset-grid techniques process and pass information only at grid boundaries. In the current approach, error corrections at all overlapped interior points are injected between grids by using a defect-correction scheme. In some cases this is found to enhance the overall accuracy of the overset-grid method. Locally refined overset grids can be used to provide an efficient solution-adaptation method. The defect correction can also be ultilized as an error-correction technique for a coarse grid by evaluating the residual using a fine base grid, but solving the implicit equations only on the coarse grid. Numerical examples include an accuracy and dissipation study of an unsteady decaying vortex flow, the flow over a NACA 0012 airfoil, and the flow over a mulit-element high-lift airfoil.

Rogers, Stuart E.↗

On-line removal of volatile fatty acids from CELSS anaerobic bioreactor via nanofiltration

The CELSS (controlled ecological life support system) resource recovery system, which is a waste processing system, uses aerobic and anaerobic bioreactors to recover plants nutrients and secondary foods from the inedible biomass. The anaerobic degradation of the inedible biomass by means of culture of rumen bacteria,generates organic compounds such as volatile fatty acids (acetic, propionic, butyric, VFA) and ammonia. The presence of VFA in the bioreactor medium at fairly low concentrations decreases the microbial population's metabolic reactions due to end-product inhibition. Technologies to remove VFA continuously from the bioreactor are of high interest. Several candidate technologies were analyzed, such as organic solvent liquid-liquid extraction, adsorption and/or ion exchange, dialysis, electrodialysis, and pressure driven membrane separation processes. The proposed technique for the on-line removal of VFA from the anaerobic bioreactor was a nanofiltration membrane recycle bioreactor. In order to establish the nanofiltration process performance variables before coupling it to the bioreactor, a series of experiments were carried out using a 10,000 MWCO tubular ceramic membrane module. The variables studied were the bioreactor slurry permeation characteristics, such as, the permeate flux, VFA and the nutrient removal rates as a function of applied transmembrane pressure, fluid recirculation velocity, suspended matter concentration, and process operating time. Results indicate that the permeate flux, VFA and nutrients removal rates are directly proportional to the fluid recirculation velocity in the range between 0.6 to 1.0 m/s, applied pressure when these are low than 1.5 bar, and inversely proportional to the total suspended solids concentration in the range between 23,466 to 34,880. At applied pressure higher than 1.5 bar the flux is not more linearly dependent due to concentration polarization and fouling effects over the membrange surface. It was also found that the permeate flux declines rapidly during the first 5 to 8 hours, and then levels off with a diminishing rate of flux decay.

Colon, Guillermo↗

A New Paradigm for Gamma Ray Bursts: Long Term Accretion Rate Modulation by an External Accretion Disk

We present a new way of looking at the very long term evolution of GRBs in which the disk of material surrounding the putative black hole powering the GRB jet modulates the mass flow, and hence the efficacy of the process that extracts rotational energy from the black hole and inner accretion disk. The pre-Swift paradigm of achromatic, shallow-to-steep "breaks" in the long term GRB light curves has not been borne out by detailed Swift data amassed in the past several years. We argue that, given the initial existence of a fall-back disk near the progenitor, an unavoidable consequence will be the formation of an "external disk" whose outer edge continually moves to larger radii due to angular momentum transport and lack of a confining torque. The mass reservoir at large radii moves outward with time and gives a natural power law decay to the GRB light curves. In this model, the different canonical power law decay segments in the GRB identified by Zhang et al. and Nousek et al. represent different physical states of the accretion disk. We identify a physical disk state with each power law segment.

Cannizzo, John↗

A numerical study of longitudinal vortex interaction with a boundary layer

A numerical study of vortex generator aerodynamics has been carried out with a reduced set of Navier-Stokes equations. These are used to model the physical process of longitudinal vortex interaction with a turbulent boundary layer on a flat plate in incompressible flow. Comparison with experimental data is used to validate the approach, and detailed predictions are made. Results show an increase in skin friction and a decrease in integral layer thicknesses where the flow is toward the wall, and the reverse where the flow is away from the wall. The major controlling parameters of vortex strength, spacing and height are each found to have optimum values for which the efficiency of boundary-layer thinning is maximized. Vortex core spreading and secondary vorticity produced in the layer are observed and studied, as well as circulation decay and other features of the flow.

Russell, D. A.↗

Synoptic Study of an X-Ray Nova in Outburst (ADP 2000)

In the completed work listed above, we present an X--ray spectral and timing analysis of 4131543-47 during its 2002 outburst based on 49 pointed observations obtained using the Rossi X-ray Timing Explorer (RXTE). The outburst reached a peak intensity of 4.2 Crab in the 2-12 keV band and declined by a factor of 32 throughout the month-long observation. A 21.9+/-0.6 mJy radio flare was detected at 1026.75 MHz two days before the X-ray maximum; the radio source was also detected late in the outburst, after the X-ray source entered the hard state. The X-ray light curve exhibits the classic shape of a rapid rise and an exponential decay. The spectrum is soft and dominated by emission from the accretion disk. The continuum is fit with a multicolor disk blackbody (kT-max = 1.04 keV) and a power-law (Gamma -2.7). Midway through the decay phase, a strong low-frequency quasi-periodic oscillation (QPO; nu = 7.3-8.1 Hz) was present for several days. The spectra feature a broad Fe K line that is asymmetric, suggesting that the line is due to relativistic broadening rather than Gomptonization. Relativistic Laor models provide much better fits to the line than non-relativistic Gaussian models, particularly near the beginning and end of our observations. The line fits yield estimates for the inner disk radius that are within 6 GM/c 2; this result and additional evidence indicates that this black hole may have a non-zero angular momentum. In current work, we are in the process of analyzing a huge set of RXTE data (-100 pointed observations) for H1743-322, which was in eruption throughout most of 2003. The source s behavior is remarkable. It displays all of the canonical X-ray states, sometimes in unusual ways. In this short report, we feature a single important result, namely, the detection of a pair of high-frequency QPOs. The bright flaring behavior is of special interest. During the flares the shape of the power spectrum and the presence of low-frequency QPOs indicate that the source is in the steep power-law (SPL or very high) state. It is in this state that the high-frequency QPOs, which are believed to emanate from the very inner accretion disk, were oberved. Specifically, we observe a 240 Hz QPO at a significance of 4.7sigma and a 162 Hz QPO at 3.7sigma. The higher frequency QPO was discovered by Homan et al. in an independent data set; they also found marginal evidence (2.5sigma)

McClintock, Jeffrey↗

The PCM is Dead! Long Live O-PCM!

The ability to reconfigure the optical behavior of a device enables free-space applications ranging from imaging to sensing and signal control. Such optical devices can be compacted via meta-surfaces, patterned structures with feature sizes below the incident wavelength. Leveraging geometry in addition to material properties and CMOS fabrication techniques has allowed meta-surfaces for lenses, holograms, beam steerers and more. To incorporate multiple optical functions into one device, various methods of device control have been implemented, such as stretching of flexible substrates, tuning the refractive index of the comprising meta-atoms via the electro-optic or the thermo-optic effects, phase transition materials such as VO2 and more. Chalcogenide glasses used as optical phase change materials, such as Ge¬2Sb2Te5 (GST), have gained increased traction in the optics community for potential use in the near infrared (NIR) and mid infrared (MIR) bands, including the telecom bands. Various chalcogenides such as Sb2Se3, Sb2S3, Ge2Sb¬2Se5 and Ge2Sb2Se4Te (GSST) have been investigated due to their broad NIR or MIR transparency window and large changes in refractive index. In their amorphous phase, these materials usually display a lower refractive index and low absorption when compared to their crystalline state which display a higher refractive index and typically larger extinction coefficients. The amorphous-crystalline reversible switching can be done via fast melt-quenching thermal processes triggered by laser or electrical impulses, relying on a substrate as a heat sink. The potential of PCMs in photonic devices can be limited by intrinsic material limitations as well as by device fabrication issues. To explore the cyclability of GSST, a PCM with large refractive index contrast and on-chip electrothermal switching on a silicon-on-insulator platform has been done to analyze potential failure mechanisms from both a material and device perspective. A brief outline of the instrumentation and phase change contrast analysis is provided. Dewetting of the PCM, delamination of and damage in the PECVD SiNx protective layer, elemental migration in the PCM and optical contrast decay have been observed in cycled GSST devices. Guidelines for device performance improvement are proposed, and an improved design with larger endurance is shown in progress.

reconfigurable photonics↗

Are large concentration of atomic H storable in tritium-impregnated solid in H2 below 0.10 K

The storage and release of atomic hydrogen produced by the beta decay of tritium contained in a crystalline solid H2 matrix at concentrations greater than 2% and temperatures below 0.80 K are investigated. The temperature of a sample chamber containing tritium-impregnated H2 and placed in the mixing chamber of a dilution refrigerator was measured as the chamber was heated and cooled in order to determine the rates of energy storage and release. It is found that for samples containing 1.2 wt.% tritium, after storage at 0.054 K for 40 h, an increase in sample temperature to a trigger point of 0.17 K leads to an energy release due to the destabilization of atomic H in H2 as predicted by the phenomenological rate process theory. For a tritium weight fraction of 2.5%, energy releases were triggered at 0.54 and 0.82 K after storage at 0.080 K, indicating the trapping of H atoms at the sites of T2 and HT molecules in the sample. The application of a 15 kG magnetic field is shown to increase the storage capacity of T2 traps while reducing that of HT traps, and to lower the trigger temperatures of both. Results suggest that the direct conversion of nuclear energy to chemical energy may become technically feasible in the future.

Rosen, G.↗

Development of an Autofluorescence Spectral Database for the Identification and Classification of Microbial Extremophiles

Extremophiles are microorganisms that have adapted to severe conditions that were once considered devoid of life. The extreme settings in which these organisms flourish on earth resemble many extraterrestrial environments. Identification and classification of extremophiles in situ (without the requirement for excessive handling and processing) can provide a basis for designing remotely operated instruments for extraterrestrial life exploration. An important consideration when designing such experiments is to prevent contamination of the environments. We are developing a reference spectral database of autofluorescence from microbial extremophiles using long-UV excitation (405 nm). Aromatic compounds are essential components of living systems, and biological molecules such as aromatic amino acids, nucleotides, porphyrins and vitamins can also exhibit fluorescence under long-UV excitation conditions. Autofluorescence spectra were obtained from a confocal microscope that additionally allowed observations of microbial geometry and motility. It was observed that all extremophiles studied displayed an autofluorescence peak at around 470 nm, followed by a long decay that was species specific. The autofluorescence database can potentially be used as a reference to identify and classify past or present microbial life in our solar system.

Sabanayagam, Chandran↗