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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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NASA NTRS2022

Quantifying Errors in 3D CME Parameters Derived From Synthetic Data Using White-Light Reconstruction Techniques

Current efforts in space weather forecasting of CMEs have been focused on predicting their arrival time and magnetic structure. To make these predictions, methods have been developed to derive the true CME speed, size, position, and mass, among others. Difficulties in determining the input parameters for CME forecasting models arise from the lack of direct measurements of the coronal magnetic fields and uncertainties in estimating the CME 3D geometric and kinematic parameters after eruption. White-light coronagraph images are usually employed by a variety of CME reconstruction techniques that assume more or less complex geometries. This is the first study from our International Space Science Institute (ISSI) team “Understanding Our Capabilities in Observing and Modeling Coronal Mass Ejections”, in which we explore how subjectivity affects the 3D CME parameters that are obtained from the Graduated Cylindrical Shell (GCS) reconstruction technique, which is widely used in CME research. To be able to quantify such uncertainties, the “true” values that are being fitted should be known, which are impossible to derive from observational data. We have designed two different synthetic scenarios where the “true” geometric parameters are known in order to quantify such uncertainties for the first time. We explore this by using two sets of synthetic data: 1) Using the ray-tracing option from the GCS model software itself, and 2) Using 3D magnetohydrodynamic (MHD) simulation data from the Magnetohydrodynamic Algorithm outside a Sphere code. Our experiment includes different viewing configurations using single and multiple viewpoints. CME reconstructions using a single viewpoint had the largest errors and error ranges overall for both synthetic GCS and simulated MHD white-light data. As the number of viewpoints increased from one to two, the errors decreased by approximately 4° in latitude, 22° in longitude, 14° in tilt, and 10° in half-angle. Our results quantitatively show the critical need for at least two viewpoints to be able to reduce the uncertainty in deriving CME parameters. We did not find a significant decrease in errors when going from two to three viewpoints for our specific hypothetical three spacecraft scenario using synthetic GCS white-light data. As we expected, considering all configurations and numbers of viewpoints, the mean absolute errors in the measured CME parameters are generally significantly higher in the case of the simulated MHD white-light data compared to those from the synthetic white-light images generated by the GCS model. We found the following CME parameter error bars as a starting point for quantifying the minimum error in CME parameters from white-light reconstructions: Δθ (latitude)=6° +2° -3° , Δϕ (longitude)=11° +18° -6° , Δγ (tilt)=25° +8° -7° , Δx (half-angle)=10° +12° -6° , Δh (height)=0.6 +1.2 -0.4 R ⨀ , and Δκ (ratio)=0.1 +0.03 -0.02 .

Coronal mass ejections↗
NASA NTRS2023

Using Orbiting Carbon Observatory-2 (OCO-2) column CO2 retrievals to rapidly detect and estimate biospheric surface carbon flux anomalies

The global carbon cycle is experiencing continued perturbations via increases in atmospheric carbon concentrations, which are partly reduced by terrestrial biosphere and ocean carbon uptake. Greenhouse gas satellites have been shown to be useful in retrieving atmospheric carbon concentrations and observing surface and atmospheric CO2 seasonal-to-interannual variations. However, limited attention has been placed on using satellite column CO2 retrievals to evaluate surface CO2 fluxes from the terrestrial biosphere without advanced inversion models at low latency. Such applications could be useful to monitor, in near real time, biosphere carbon fluxes during climatic anomalies like drought, heatwaves, and floods, before more complex terrestrial biosphere model outputs and/or advanced inversion modelling estimates become available. Here, we explore the ability of Orbiting Carbon Observatory-2 (OCO-2) column-averaged dry air CO2 (XCO2) retrievals to directly detect and estimate terrestrial biosphere CO2 flux anomalies using a simple mass-balance approach. An initial global analysis of surface–atmospheric CO2 coupling and transport conditions reveals that the western US, among a handful of other regions, is a feasible candidate for using XCO2 for detecting terrestrial biosphere CO2 flux anomalies. Using the CarbonTracker model reanalysis as a test bed, we first demonstrate that a well-established mass-balance approach can estimate monthly surface CO2 flux anomalies from XCO2 enhancements in the western United States. The method is optimal when the study domain is spatially extensive enough to account for atmospheric mixing and has favorable advection conditions with contributions primarily from one background region. We find that errors in individual soundings reduce the ability of OCO-2 XCO2 to estimate more frequent, smaller surface CO2 flux anomalies. However, we find that OCO-2 XCO2 can often detect and estimate large surface flux anomalies that leave an imprint on the atmospheric CO2 concentration anomalies beyond the retrieval error/uncertainty associated with the observations. OCO-2 can thus be useful for low-latency monitoring of the monthly timing and magnitude of extreme regional terrestrial biosphere carbon anomalies.

Andrew F. Feldman↗
NASA NTRSDate not supplied

An Approach to Shape Parameterization Using Laboratory Hypervelocity Impact Experiments

NASA’s Orbital Debris Program Office relies on laboratory-based impact tests to supplement the measurement data of on-orbit events that defines the orbital debris environment. These experiments provide information that is essential to interpreting the radar and optical measurements of orbital fragmentation events into useful metrics, such as characteristic size of the debris, and to providing a better understanding of the distributions of fragment populations in terms of their masses, material constituents, fragment densities, cross-sectional areas, area-to-mass ratios, shapes, etc. The Satellite Orbital Debris Characterization Impact Test (SOCIT) was a notable laboratory impact experiment conducted in 1992 using a surplus U.S. Navy Transit navigation satellite of the 1960s. The data from this ground-based experiment were combined with on-orbit measurements to develop the NASA Standard Satellite Breakup Model (SSBM). To account for advancements in satellite design and construction since, a new impact test series – DebriSat – was conducted in 2014. This test utilized a high-fidelity mock-up spacecraft that better represents the materials and construction techniques used to design and manufacture modern spacecraft. Together, these tests offer valuable data to model an orbital debris environment composed of legacy and modern spacecraft. This paper presents an overview of the two laboratory impact tests, comparing their fragment parameter distributions with each other and with relevant distributions from the NASA SSBM. The categorization and descriptions of fragment shapes are of significant interest for future work, yet there are marked differences in the definitions of shape categories between each dataset. The categorizations of constituent materials, and the measurement techniques employed to populate these two datasets, are also different. New rubrics simplify and equate the categorizations between datasets to aid comparative analyses and to facilitate the potential use of both datasets in tandem with future environmental debris models. A preferred approach to classifying shape across disparate datasets uses the characteristic-length dimensions, and a simplified shape classification based on physical, solid-body dimensions, to mathematically construct an encapsulating right-circular cylinder that represents the fragment. The ratio of cylinder length-to-diameter (L:D) then provides a single continuum value for shape that is strongly correlated with its designated shape and size. This metric can then be used to further assess the distribution of shape with populations of other fragment characteristics within these datasets. The shape parameterization using the L:D ratios of right-circular cylinders is discussed.

John H Seago↗
NASA NTRS2024

Discrete Event Simulation-Based Timeline Validation Using R2U2

The Gateway Vehicle Systems Manager (VSM), the top-level software control system in a distributed, hierarchical Autonomous System Management Architecture is, like most modern spacecraft software control systems, heavily data-driven. For example, schedules (timelines) will be developed on the ground and, due to the high degree of autonomy, contain complex procedures involving conditional branching, variable timing, and resource contention resolution. In order to verify that an uploaded timeline will function correctly, it is necessary to explore the feasible set of possible executions. While it is possible to test a timeline using a mission simulation, the complexity of the system and duration of a timeline limits the number of trials and therefore the test coverage. To address this problem, the VSM team is using a discrete event system model that can rapidly generate from a timeline sets of event sequences using Monte Carlo techniques. To achieve rapid and trustworthy checking of the event sequences, we use an offline version of the runtime model checking tool R2U2. This presentation describes the approach the VSM team is using to implement the discrete event simulation and evaluate event sequences using R2U2. The presentation will discuss: 1. Description of the timelines by VSM in the context of VSM operations 2. Expansion of a timeline into a sequence of atomic events 3. Adjustment, in the Monte Carlo environment, of an event sequence to account for uncertainty, external events, and failures 4. Definition of R2U2 input and mission-time linear temporal logic files 5. Generation and use of R2U2 verdict sequences 6. Lessons learned and future work

Verification↗
NASA NTRSDate not supplied

Nodal Modeling of Tank Pressurization and Draining using a Multi-Node-Ullage Approach

The purpose of the pressurization system in liquid rocket propulsion is to control the pressure in the gas space of the propellant tank (known as the ullage space) and the propellant mass flowrate to the engine. A mathematical model is required to predict the amount of pressurant necessary to ensure that pressure and temperature levels inside the tank remain within acceptable limits and that the propellant pressure leaving the tank satisfies the net positive suction pressure (NPSP) requirement of the pump feeding the engine. Nodal codes typically model tank pressurization and draining using a single node to represent the ullage and a single node to represent the propellant. As the tank drains, the ullage node grows and the propellant node shrinks. The heat transfer between ullage to wall and ullage to propellant is governed by natural convection. Designers of liquid propulsion systems often use empirical correlations to estimate the “Collapse Factor” which represents the ratio of pressurant required with heat transfer and the amount of pressurant required without heat transfer. A single node ullage model of tank pressurization was developed using GFSSP to compute the collapse factor reasonably well and later was used to model tank pressurization during test firing of the FASTRAC rocket engine. The predicted tank pressure compared well with the test data. In the early 1970’s, pressurization and drain tests with liquid methane were performed at NASA Lewis Research Center in a vacuum chamber. A 5 ft diameter spherical aluminum tank was tested to drain from 95% to 5% full using gaseous helium, hydrogen, nitrogen and methane as pressurant. Tests were conducted with different pressurant inlet temperatures and drain times. Measured data include pressurant requirement, amount of pressurant condensed, and ullage and wall temperatures at various heights in the ullage space at the end of draining. A single node GFSSP model was developed to simulate helium pressurization of the methane tank. Predicted helium consumption was 8-23% less than measured. The average error of the six test cases was 16%. A single ullage node with multiple solid node model was developed using Thermal Desktop. Predicted helium consumption compares with the test data within 2%. This paper describes the development of a GFSSP multi-node ullage model of the test configuration and compares the predicted pressurant consumption for both helium and autogenous pressurization using gaseous methane with experimental as well as TD predictions.

Nodal Model↗
NASA NTRS2024

Critical Load Exceedances for North America and Europe using an Ensemble of Models and an Investigation of Causes for Environmental Impact Estimate Variability: An AQMEII4 Study

Exceedances of critical loads for deposition of sulphur (S) and nitrogen (N) to different ecosystems were estimated using European and North American ensembles of air quality models, under Phase 4 of the Air Quality Model Evaluation International Initiative (AQMEII4), to identify where risk of ecosystem harm is expected to occur based on model deposition estimates. The ensembles were driven by common emissions and lateral boundary condition inputs. Model output was regridded to common North American and Europe 0.125° resolution domains, which were then used to calculate critical load exceedances. New, targeted deposition diagnostics implemented in AQMEII4 allowed an unprecedented level of post-simulation analysis to be carried out and facilitated the identification of specific causes of model-to-model variability in critical load exceedance estimates. New datasets for North American critical loads for acidity for forest soil water and aquatic ecosystems were combined with the ensemble deposition predictions to show a substantial decrease in the area and number of locations in exceedance between 2010 and 2016 (forest soils: 13.2 % to 6.1 %; aquatic ecosystems: 21.2 % to 11.4 %). All models agreed in the direction of the ensemble exceedance change between 2010 and 2016. The North American ensemble also predicted a decrease in both severity and total area in exceedance between the years 2010 and 2016 for eutrophication-impacted ecosystems in the USA (sensitive epiphytic lichen: 81.5 % to 75.8 %). The exceedances for herbaceous community richness also decreased between 2010 and 2016, from 13.9 % to 3.9 %. The uncertainty associated with the North American eutrophication results is high; there were sharp differences between the models in both predictions of total N deposition and the change in N deposition, and hence in the predicted eutrophication exceedances between the two years. The European ensemble was used to predict relatively static exceedances of critical loads with respect to acidification (4.48 % to 4.32 % from 2009 to 2010) while eutrophication exceedance increased slightly (60.2 % to 62.2 %). While most models showed the same changes in critical load exceedances as the ensemble between the two years, the spatial extent and magnitude of exceedances varied significantly between the models. The reasons for this variation were examined in detail by first ranking the relative contribution of different sources of sulphur and nitrogen deposition in terms of deposited mass and model-to-model variability in that deposited mass, followed by their analysis using AQMEII4 diagnostics, along with evaluation of the most recent literature. All models in both the North American and European ensembles had net annual negative biases with respect to observed wet deposition of sulphate, nitrate and ammonium. Diagnostics and recent literature suggest that this bias may stem from insufficient cloud scavenging of aerosols and gases, and may be improved through the incorporation of multiphase hydrometeor scavenging within the modelling frameworks. The inability of North American models to predict the timing of the seasonal peak in wet ammonium ion deposition (observed maximum was in April, while all models predicted a June maximum) may also relate to the need for multiphase hydrometeor scavenging (absence of snow scavenging in all models employed here). High variability in the relative importance of particulate sulphate, nitrate and ammonium deposition fluxes between models was linked to the use of updated particle dry deposition parameterizations in some models. However, recent literature and further development of some of the models within the ensemble suggests these particulate biases may also be ameliorated via the incorporation of multiphase hydrometeor scavenging. Annual sulphur and nitrogen deposition prediction variability was linked to SO 2 and HNO 3 dry deposition parameterizations, and diagnostic analysis showed that the cuticle and soil deposition pathways dominate the deposition mass flux of these species. Further work improving parameterizations for these deposition pathways should reduce variability in model acidifying gas deposition estimates. The absence of base cation chemistry in some models was shown to be a major factor in positive biases in fine mode particulate ammonium and particle nitrate concentrations. Models employing ammonia bidirectional fluxes had both the largest and the smallest magnitude biases, depending on the model and bidirectional flux algorithm employed. A careful analysis of bidirectional flux models suggests that those with poor NH 3 performance may underestimate the extent of NH 3 emissions fluxes from forested areas. Based on these results, an increased process-research focus is therefore recommended for the following model processes and on observations which may assist in model evaluation and improvement: multiphase hydrometeor scavenging combined with updated particle dry deposition, cuticle and soil deposition pathway algorithms for acidifying gases, base cation chemistry and emissions, and NH 3 bidirectional fluxes. Comparisons with satellite observations suggest that oceanic NH 3 emissions sources should be included in regional chemical transport models. The choice of land use database employed within any given model was shown to significantly influence deposition totals in several instances, and employing a common land use database across chemical transport models and critical load calculations is recommended for future work.

critical loads↗
NASA NTRSDate not supplied

Using Thermal Desktop to Determine Equivalent Solar Hours on Spacecraft Surfaces

Equivalent solar hours (ESH) are the duration of exposure of a surface to solar radiation, particularly ultraviolet (UV) radiation. Since UV light causes degradation to optical properties of thermal control surfaces, the determination of ESH is important to calculate end of life (EOL) optical properties for spacecraft external surfaces. A higher ESH value generally correlates to greater UV degradation experienced by the surface, resulting in an increase in solar absorptivity. This paper details a novel approach for calculating ESH using Thermal Desktop. Since the ESH value is useful for thermal analysts, this technique is beneficial to quickly perform the analysis internally with tools readily available without relying on external support and funding. The example presented in this paper shows how this method was developed and used for NASA's lunar Gateway spacecraft. This method of analysis is used to calculate ESH for the transit, assembly in orbit, and operational phases for Gateway, utilizing each of the program’s planned configurations throughout its 15-year lifetime. ESH values were calculated using the Monte-Carlo ray tracing capability of Thermal Desktop using both direct solar heating and indirect solar heating due to reflections from other surfaces. The output of this analysis includes an ESH gradient map across the spacecraft. Post-processing of these results enabled identification of the average and maximum ESH on critical surfaces such as radiators. The final ESH values for each surface are then used for determination of EOL optical properties.

Thermal Control↗
NASA NTRSDate not supplied

Simulating High Energy Dynamic Impact of IM7/PEKK Continuous Fiber Laminated Thermoplastic Composite using Open Hole Coupon Experiments

The use of advanced thermoplastic composites (TPC) for structural applications in the aerospace and automotive industries has grown increasingly popular due to their high performance, high manufactured part output, and sustainability. Enhancing verification simulations of the progressive failure in TPCs at the coupon scale is needed to better validate advanced composite material models at larger length scales, such as element level panels. MAT_213, a tabulated composite material model, has been applied to simulate high velocity dynamic impact (HEDI) and is integrated in the LS-DYNA explicit finite element (FE) software. Previous efforts using MAT_213 have calibrated HEDI simulated failures with experimental data. Traditionally simulations have relied on a structured mesh to represent quasi-isotropic panels. For this study, a continuous fiber unidirectional tape, IM7/PEKK, was analyzed through a series of verification studies of notched laminate coupons, then validation analyses of quasi-isotropic panels under HEDI were compared with experimental data. Experiments using unidirectional TPC were performed to obtain tabulated stress vs strain curves using Digital Image Correlation (DIC) for input into MAT_213. Next, notched coupon experiments were used as an intermediate step to calibrate the damage and failure behavior in MAT_213. Lastly, investigations into differences between HEDI simulations using a fiber aligned mesh were compared with experimental failure patterns and damage sizes. The use of experimentally generated material behavior along with selective mesh layout improved HEDI predictions with minimal material parameter calibration.

Polymer matrix composites↗
NASA NTRSDate not supplied

Improved Assessment of Recent Trends in NOx and VOC Emissions and Ozone Production Sensitivity Regimes Using Satellite Data

This presentation highlights results from a NASA Aura Science Team and Atmospheric Composition Modeling and Analysis Program (ACMAP) project which study the capability to observe and model trends in ozone (O3) production regimes using spaceborne sensors. Ultraviolet– visible (UV–Vis) tropospheric column satellite retrievals of formaldehyde (HCHO) (a proxy for volatile organic compound [VOC] reactivity) and nitrogen dioxide (NO2) (a proxy for nitrogen oxides [NOx]) are frequently used to investigate the sensitivity of O3 production to emissions of NOx and VOCs. There are challenges that come from using satellite-derived ratios of HCHO and NO2 (FNR) to study O3 production sensitivity with the largest uncertainties associated with specific spaceborne sensor’s retrieval biases and errors. This study quantifies the differences and improvements in satellite retrievals of O3 production sensitivity regimes using FNRs when moving from legacy polar orbiting satellites such as the Ozone Monitoring Instrument (OMI) onboard NASA’s Aura satellite and Ozone Mapping and Profiler Suite Nadir Mapper (OMPS-NM) onboard the NASA/NOAA Suomi-NPP platform to newer, higher spatiotemporal resolution satellite sensors TROPOspheric Monitoring Instrument (TROPOMI) and eventually the recently launched NASA geostationary sensor Tropospheric Emissions: Monitoring of Pollution (TEMPO). Furthermore, we investigate how using retrievals of NO2 and HCHO from these different satellites to constrain model predictions impacts the ability to accurately simulate O3 chemistry including chemical production regimes. To this end, we have conducted inverse model simulations, using the WRF-CMAQ-DDM data assimilation system at 12 km × 12 km, to constrain emissions of NOx and VOCs over the contiguous United States (CONUS) when assimilating OMI and TROPOMI retrievals of NO2 and HCHO. Two advantages of this are that we a) account for each satellite’s errors/biases in the emission estimation and b) update the prior profile to ensure that only radiance information is used for optimizing the emissions. This presentation will demonstrate: a) the varying accuracy of different satellite retrieved FNRs and ability to capture known sub-annual emission trends (e.g., seasonal, weekend/weekday) and emission anomalies during the COVID-19 lockdown of 2020, b) the differences and improvements in top-down emission estimates of NOx and VOCs when constrained by newer satellite sensors compared to legacy systems, and c) multi-sensor optimized emission estimates of summer-time NOx and VOCs between 2019-2021.

Data↗
NASA NTRSDate not supplied

Investigating the Impacts of Land Use Change on Urban Heat and Vulnerability in Cali, Columbia

The urban heat island effect (UHI) is an environmental phenomenon where cities experience higher temperatures than rural areas due to increased pavement and decreased cooling from vegetation. Approximately 76% of people in Colombia live in urban areas, and the city of Santiago de Cali is facing UHI challenges exacerbated by land use change. Wetlands and forests formerly surrounded the city but were replaced by development and agriculture. The Colombian municipal government agency Departamento Administrativo de Gestión del Medio Ambiente and the community organization Fundacion Dinamizadores Ambientales partnered with NASA DEVELOP to evaluate communities in Cali most vulnerable to urban heat. This project illustrated the utility of using NASA Earth observations to evaluate the relationship between land use, temperature, and social factors in Cali, Colombia between 2013 and 2023. The team used Landsat 7 Enhanced Thematic Mapper Plus (ETM+), Landsat 8 Operational Land Imager (OLI) and Thermal Infrared Sensor (TIRS), and Landsat 9 OLI-2/TIRS-2 to generate land surface temperature (LST), normalized difference vegetation index (NDVI), and albedo maps in Google Earth Engine. Heavy cloud cover limited the accuracy of the LST but incorporating up to three satellites for a median image reduced potential errors. Through further analysis in ArcGIS Pro, the team classified land use change using a deep learning model and found that LST was significantly higher in urban areas than in wetlands or forests. Using R studio, the team ran a principal component analysis to determine which social factors had the strongest correlation with LST. The team found that health care and green space access were negatively correlated, and Afro-Colombian ethnicity was positively correlated with LST. With awareness of the most impacted and vulnerable regions, the partner organizations can work to prioritize green space establishment in those areas to reduce the impacts of urban heat. Addressing the urban heat island effect will reduce environmental justice concerns within the city and improve overall health, air, and water quality for those who live there.

Brenna Bruffey↗
NASA NTRSDate not supplied

Variable Lidar Ratios for Marine and Dusty Marine Using MODIS AOD Constrained Retrievals and GOCART Model Simulations and their Impact on Level 2 CALIPSO Data Products in Version 5

The Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) onboard CALIPSO provided global measurements of attenuated backscatter profiles of various tropospheric aerosol species from June 2006 through June 2023. Extinction profiles are retrieved from these backscatter measurements by assuming a value for the extinction-to-backscatter or lidar ratio (LR). Up until version V4.51 of the data products, only a constant global value for each of the species has been used, spatially and temporally. However, it has been well known from various ground-based measurements that LRs of each aerosol species can potentially vary spatially as well seasonally. For the forthcoming Version 5 of the CALIPSO products, the CALIPSO team will implement variable lidar ratios at 532 nm for the species classified as “marine” and “dusty marine”. Here we present the essential elements of this scheme, which involves estimating lidar ratios by constraining Fernald solutions for the CALIOP particulate backscatter profiles using aerosol optical depths (AOD) from collocated MODIS retrievals. To account for the lack of measurements in various regions and seasons (e.g., Arctic winters), we leverage collocated model sea-salt volume fractions (SSVF) simulated by the Goddard Chemistry Aerosol Radiation and Transport (GOCART) model and use an empirical relationship between the SSVF and LR to build hybrid climatological maps for the entire globe, i.e., using the modelled values when reliable values of the constrained LRs are not available. Initial evaluation of the resulting extinction profiles and corresponding AODs, both globally and in specific regions are presented using a limited amount of data. The primary improvement in AOD occurs in the coastal areas, like the Arabian Sea and Bay of Bengal. The estimated marine LR values in these regions are significantly higher than the constant value of 23 sr used in previous data releases, likely due to mixing with offshore pollution which results in higher AODs. Initial validation results using the CALIPSO Ocean Derived Column Optical Depth (ODCOD) retrievals and ground-based AERONET AOD measurements are presented.

CALIPSO↗
NASA NTRS1999

Detection of endogenous alkaline phosphatase activity in intact cells by flow cytometry using the fluorogenic ELF-97 phosphatase substrate

BACKGROUND: The alkaline phosphatase (AP) substrate 2-(5'-chloro-2'-phosphoryloxyphenyl)-6-chloro-4-(3H)-quinazolinone (ELF((R))-97 for enzyme-labeled fluorescence) has been found useful for the histochemical detection of endogenous AP activity and AP-tagged proteins and oligonucleotide probes. In this study, we evaluated its effectiveness at detecting endogenous AP activity by flow cytometry. METHODS: The ELF-97 phosphatase substrate was used to detect endogenous AP activity in UMR-106 rat osteosarcoma cells and primary cultures of chick chondrocytes. Cells were labeled with the ELF-97 reagent and analyzed by flow cytometry using an argon ultraviolet (UV) laser. For comparison purposes, cells were also assayed for AP using a Fast Red Violet LB azo dye assay previously described for use in detecting AP activity by flow cytometry. RESULTS: The ELF-97 phosphatase substrate effectively detected endogenous AP activity in UMR-106 cells, with over 95% of the resulting fluorescent signal resulting from AP-specific activity (as determined by levamisole inhibition of AP activity). In contrast, less than 70% of the fluorescent signal from the Fast Red Violet LB (FRV) assay was AP-dependent, reflecting the high intrinsic fluorescence of the unreacted components. The ELF-97 phosphatase assay was also able to detect very low AP activity in chick chondrocytes that was undetectable by the azo dye method. CONCLUSIONS: The ELF-97 phosphatase assay was able to detect endogenous AP activity in fixed mammalian and avian cells by flow cytometry with superior sensitivity to previously described assays. This work also shows the applicability of ELF-97 to flow cytometry, supplementing its previously demonstrated histochemical applications. Copyright 1999 Wiley-Liss, Inc.

Non-NASA Center↗
NASA NTRS1975

CARETS: A prototype regional environmental information system. Volume 7: Land use information and air quality planning

The pilot national land use information system developed by the U.S. Geological Survey in the Central Atlantic Regional Ecological Test Site project has provided an improved technique for estimating emissions, diffusion, and impact patterns of sulfur dioxide (S0 2 ) and particulate matter. Implementation of plans to control air quality requires land use information, which, until this time, has been inadequate. The pilot system, however, provided data for updating information on the sources of point and area emissions of S0 2 and particulate matter affecting the Norfolk--Portsmouth area of Virginia for the 1971-72 winter (Dec.-Jan.-Feb.) and the annual 1972 period, and for a future annual period--1985. This emission information is used as input to the Air Quality Display Model of the Environmental Protection Agency to obtain diffusion and impact patterns for the three periods previously mentioned. The results are: (1) During the 1971-72 winter, estimated SO 2 amounts over an area with a SW-NE-axis in the central section of Norfolk exceeded both primary and secondary levels; (2) future annual levels of SO 2 , estimated by anticipated residential development and point-source changes, are not expected to cause serious deterioration of the region's present air quality; and (3) for the 1971-72 winter and annual 1972 period the diffusion results showed that both primary and secondary standards for particulate matter are regularly exceeded in central Norfolk and Portsmouth. In additions on the bas is of current control programs, the 1985 levels of particulate matter are expected to exceed the presently established secondary air quality standards throughout central Norfolk and Portsmouth and in certain areas of Virginia Beach. The land use information can be used to estimate emissions for inputs to diffusion models and to interpret the implications of diffusion patterns for: (1) Implementing various control strategies (2) selecting sites of air sampling stations, and (3) predicting the effects that proposed changes in land use might have on emission patterns and air quality.

Remote sensing↗
NASA NTRS2005

Use of animal models for space flight physiology studies, with special focus on the immune system

Animal models have been used to study the effects of space flight on physiological systems. The animal models have been used because of the limited availability of human subjects for studies to be carried out in space as well as because of the need to carry out experiments requiring samples and experimental conditions that cannot be performed using humans. Experiments have been carried out in space using a variety of species, and included developmental biology studies. These species included rats, mice, non-human primates, fish, invertebrates, amphibians and insects. The species were chosen because they best fit the experimental conditions required for the experiments. Experiments with animals have also been carried out utilizing ground-based models that simulate some of the effects of exposure to space flight conditions. Most of the animal studies have generated results that parallel the effects of space flight on human physiological systems. Systems studied have included the neurovestibular system, the musculoskeletal system, the immune system, the neurological system, the hematological system, and the cardiovascular system. Hindlimb unloading, a ground-based model of some of the effects of space flight on the immune system, has been used to study the effects of space flight conditions on physiological parameters. For the immune system, exposure to hindlimb unloading has been shown to results in alterations of the immune system similar to those observed after space flight. This has permitted the development of experiments that demonstrated compromised resistance to infection in rodents maintained in the hindlimb unloading model as well as the beginning of studies to develop countermeasures to ameliorate or prevent such occurrences. Although there are limitations to the use of animal models for the effects of space flight on physiological systems, the animal models should prove very valuable in designing countermeasures for exploration class missions of the future.

Review↗
NASA NTRS2015

Simulated Impacts of Climate Change on Water Use and Yield of Irrigated Sugarcane in South Africa

Reliable predictions of climate change impacts on water use, irrigation requirements and yields of irrigated sugarcane in South Africa (a water-scarce country) are necessary to plan adaptation strategies. Although previous work has been done in this regard, methodologies and results vary considerably. The objectives were (1) to estimate likely impacts of climate change on sugarcane yields, water use and irrigation demand at three irrigated sugarcane production sites in South Africa (Malelane, Pongola and La Mercy) for current (1980-2010) and future (2070-2100) climate scenarios, using an approach based on the Agricultural Model Inter-comparison and Improvement Project (AgMIP) protocols; and (2) to assess the suitability of this methodology for investigating climate change impacts on sugarcane production. Future climate datasets were generated using the Delta downscaling method and three Global Circulation Models (GCMs) assuming atmospheric CO2 concentration [CO2] of 734 ppm(A2 emissions scenario). Yield and water use were simulated using the DSSAT-Canegro v4.5 model. Irrigated cane yields are expected to increase at all three sites (between 11 and 14%), primarily due to increased interception of radiation as a result of accelerated canopy development. Evapotranspiration and irrigation requirements increased by 11% due to increased canopy cover and evaporative demand. Sucrose yields are expected to decline because of increased consumption of photo-assimilate for structural growth and maintenance respiration. Crop responses in canopy development and yield formation differed markedly between the crop cycles investigated. Possible agronomic implications of these results include reduced weed control costs due to shortened periods of partial canopy, a need for improved efficiency of irrigation to counter increased demands, and adjustments to ripening and harvest practices to counter decreased cane quality and optimize productivity. Although the Delta climate data downscaling method is considered robust, accurate and easily-understood, it does not change the future number of rain-days per month. The impacts of this and other climate data simplifications ought to be explored in future work. Shortcomings of the DSSAT-Canegro model include the simulated responses of phenological development, photosynthesis and respiration processes to high temperatures, and the disconnect between simulated biomass accumulation and expansive growth. Proposed methodology refinements should improve the reliability of predicted climate change impacts on sugarcane yield.

Climate change↗
NASA NTRS2016

Uncertainty of Wheat Water Use: Simulated Patterns and Sensitivity to Temperature and CO2

Projected global warming and population growth will reduce future water availability for agriculture. Thus, it is essential to increase the efficiency in using water to ensure crop productivity. Quantifying crop water use (WU; i.e. actual evapotranspiration) is a critical step towards this goal. Here, sixteen wheat simulation models were used to quantify sources of model uncertainty and to estimate the relative changes and variability between models for simulated WU, water use efficiency (WUE, WU per unit of grain dry mass produced), transpiration efficiency (Teff, transpiration per kg of unit of grain yield dry mass produced), grain yield, crop transpiration and soil evaporation at increased temperatures and elevated atmospheric carbon dioxide concentrations ([CO2]). The greatest uncertainty in simulating water use, potential evapotranspiration, crop transpiration and soil evaporation was due to differences in how crop transpiration was modelled and accounted for 50 of the total variability among models. The simulation results for the sensitivity to temperature indicated that crop WU will decline with increasing temperature due to reduced growing seasons. The uncertainties in simulated crop WU, and in particularly due to uncertainties in simulating crop transpiration, were greater under conditions of increased temperatures and with high temperatures in combination with elevated atmospheric [CO2] concentrations. Hence the simulation of crop WU, and in particularly crop transpiration under higher temperature, needs to be improved and evaluated with field measurements before models can be used to simulate climate change impacts on future crop water demand.

Sensitivity↗
NASA NTRS2018

Developing Land Use Land Cover Maps for the Lower Mekong Basin to Aid Hydrologic Modeling and Basin Planning

This paper discusses research methodology to develop Land Use Land Cover (LULC) mapsfor the Lower Mekong Basin (LMB) for basin planning, using both MODIS and Landsat satellitedata. The 2010 MODIS MOD09 and MYD09 8-day reflectance data was processed into monthlyNDVI maps with the Time Series Product Tool software package and then used to classify regionallycommon forest and agricultural LULC types. Dry season circa 2010 Landsat top of atmosphere reflectance mosaics were classified to map locally common LULC types. Unsupervised ISODATAclustering was used to derive most LULC classifications. MODIS and Landsat classifications werecombined with GIS methods to derive final 250-m LULC maps for Sub-basins (SBs) 1–8 of the LMB.The SB 7 LULC map with 14 classes was assessed for accuracy. This assessment compared randomlocations for sampled types on the SB 7 LULC map to geospatial reference data such as Landsat RGBs,MODIS NDVI phenologic profiles, high resolution satellite data, and Mekong River Commissiondata (e.g., crop calendars). The SB 7 LULC map showed an overall agreement to reference data of~81%. By grouping three deciduous forest classes into one, the overall agreement improved to ~87%.The project enabled updated regional LULC maps that included more detailed agriculture LULCtypes. LULC maps were supplied to project partners to improve use of Soil andWater AssessmentTool for modeling hydrology and water use, plus enhance LMB water and disaster managementin a region vulnerable to flooding, droughts, and anthropogenic change as part of basin planningand assessment.

land use land cover mapping; SWAT hydrologic model↗
NASA NTRS1973

Land use mapping and modelling for the Phoenix Quadrangle

The author has identified the following significant results. Comparison of 9 x 9 MSS band images and color composites made from bands 4, 5, and 6 showing vegetated areas near Phoenix during the summer and fall seasons aided in definitely establishing that certain land areas were being used as agricultural land and not as rangeland. Agricultural land, which appeared to be fallow, idle, or not irrigated, often became more readily identifiable as agricultural land when comparing different images of identical land areas which have been affected by seasonal vegetation changes. Experimentation with color density slicing portions of 9 x 9 MSS band 7 transparency showing the central urban core of phoenix enabled dense commercial and industrial areas to be separated from less dense urbanized land uses; however, loss of resolution produced results of limited usefulness. The best results in agricultural areas near Sun City were obtained using MSS band 5 imagery. Discrimination of different land uses in both urban and agricultural areas which were color density sliced was not possible to the degree of accuracy necessary to make mapping feasible. Examination of MSS transparencies and color composites allowed updating of a map of land use change in the Phoenix Quadrangle.

Place, J. L.↗