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Data Transferability and Collection Consistency in Marine Renewable Energy

Concerns about the potential effects of tidal turbines and wave energy devices on the marine environment continue to slow siting and permitting/consenting of single devices and arrays worldwide. While research studies and early results from post-installation monitoring over the past decade have informed interactions between marine renewable energy (MRE) devices, marine animals, and habitats, regulators still demonstrate considerable reluctance to accelerate the permitting/consenting process for devices and arrays. Furthermore, the MRE industry is struggling with the high costs of baseline assessments and post-installation monitoring, as well as long timelines for obtaining permits, which leads to uncertainty and risk related to project financing. Regulators require assessment and monitoring information to allow them to carry out the necessary analyses to describe, permit/consent, and manage the environmental risks associated with new MRE technologies and new uses of ocean space. One way to reduce risks to the industry and the environment and to allow for acceleration of the permitting/consenting process could be to transfer learning, analyses, and data sets from one country to another, among projects, and across jurisdictional boundaries. In addition, data are collected around early-stage MRE devices using many different methods, instruments, and measurement scales. If similar parameters and accessible methods of data collection were used for baseline assessments and post-installation monitoring around all early-stage devices and MRE developments, the results would be more readily comparable. This comparability would lead to a decrease in scientific uncertainty and support a common understanding of the risk of MRE devices to the marine environment. This in turn would facilitate more efficient and shorter permitting/consenting processes, which would decrease the financial risk for MRE project development. As a means of addressing the concept of transferring data (information, learning, analyses, and data sets) among projects and collecting data consistently, Annex IV has developed a data transferability process that has been socialized with the MRE community, which includes regulators, industry, developers, consultants, and researchers. The data transferability process consists of five components: 1. A Data Transferability Framework brings together data sets in an organized fashion, compares the applicability of each data set for use on other projects, and guides the process of data transfer 2. A Data Collection Consistency Table provides preferred measurement methods or processes, reporting units, and the most common methods of analysis or interpretation and use of data 3. A Monitoring Data Sets Discoverability Matrix allows a practitioner to discover data sets based on the approach presented in the Framework 4. Best Management Practices (BMPs) include five BMPs related to data transferability and collection consistency 5. An Implementation Plan presents an approach for implementing and applying the data transferability process. This report documents the background and development of the data transferability process and associated components and summarizes the next steps needed to successfully implement and apply the data transferability process.

16 TIDAL AND WAVE POWER↗

Mitigation of Radioactive Waste By Sampling and Analyzing The Affected Area and Efficient Use of Different Detectors - 17400

The paper summarizes the way by which the mitigation of the radioactive waste during events like decommissioning of nuclear power plants, or during a nuclear disaster can be done so that the rehabilitation process for flora and fauna can begin in a natural manner. The paper describes the problems faced during ER operations in the developing countries and need of international committees in these areas. The paper suggests marking of the radioactive areas which can be used for controlled personnel access and which can be useful for monitoring purposes, while also focusing on the need of proper knowledge while handling detectors in ER operations for successful site characterization. It also highlights the importance of bio-indicators in these operations and also gives a description of various detectors used widely and their related significance for ER operations. The paper gives an overview of the planning and site characterization stages that follow in the environmental remediation. Planning stage should consider: 1. The impact on the ecosystem and human beings and the risk of spreading of the radionuclides. 2. The financial aid in hand for detectors, machinery and skilled labor. As the planning stages develop, the very first thing is the survey of the site. Site investigation should be carried out in all aspects including meteorology, geology, and hydrology, seismicity, geomorphology and the other aspects depending on site for a certain period of time. A site characterization should take: 1. The amount of damage occurred to the environment in the nearby region. 2. The distribution, character and the extent to which radioactivity has been dispersed. 3. The amount of risk that will be involved for personnel working in the field. 4. The precision of data collected that is to be used as input for further processes. In the developing countries, the central governmental bodies play a pivotal role in providing funds internally or from international bodies. The paper shows the effect how political issues effect the work progress of ER operations, that hamper the process and make people frustrated, who really work on the field. Paper provides the suggestion for the proper use of detectors in these countries where surveyors lack knowledge on what kind of detectors to be used while taking precision as a parameter. The table beside gives just a small picture of the large variety of detectors available today and tries to summarizes the main detectors with applications. There should also be a proper marking of the areas into different levels of radiation that will be helpful for work access control and monitoring personnel health. The paper also suggests of using bio-indicators in ER operation more often. The paper proposes to use site-specific bio-indicators which are very helpful in knowing the proper effect of the event in the environment. There is no single paved path for every situation that can lead to a successful ER. The planning should be done in such a way that the future work is not disturbed due to technical problems. In developing countries, political issues can hamper the progress of work and time remains a critical issue in such areas. The lack of proper knowledge for the use of detectors can affect the outcome of site characterization. Areas of the survey should be marked with different levels of activity for efficient monitoring of personnel's. The use of bio-indicators should be used more often and should be chosen as per the site. As they are a helpful tool in evaluating the work progress. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

RNAi and genome editing of sugarcane: Progress and prospects

SUMMARY Sugarcane, which provides 80% of global table sugar and 40% of biofuel, presents unique breeding challenges due to its highly polyploid, heterozygous, and frequently aneuploid genome. Significant progress has been made in developing genetic resources, including the recently completed reference genome of the sugarcane cultivar R570 and pan‐genomic resources from sorghum, a closely related diploid species. Biotechnological approaches including RNA interference (RNAi), overexpression of transgenes, and gene editing technologies offer promising avenues for accelerating sugarcane improvement. These methods have successfully targeted genes involved in important traits such as sucrose accumulation, lignin biosynthesis, biomass oil accumulation, and stress response. One of the main transformation methods—biolistic gene transfer or Agrobacterium ‐mediated transformation—coupled with efficient tissue culture protocols, is typically used for implementing these biotechnology approaches. Emerging technologies show promise for overcoming current limitations. The use of morphogenic genes can help address genotype constraints and improve transformation efficiency. Tissue culture‐free technologies, such as spray‐induced gene silencing, virus‐induced gene silencing, or virus‐induced gene editing, offer potential for accelerating functional genomics studies. Additionally, novel approaches including base and prime editing, orthogonal synthetic transcription factors, and synthetic directed evolution present opportunities for enhancing sugarcane traits. These advances collectively aim to improve sugarcane's efficiency as a crop for both sugar and biofuel production. This review aims to discuss the progress made in sugarcane methodologies, with a focus on RNAi and gene editing approaches, how RNAi can be used to inform functional gene targets, and future improvements and applications.

Brant, Eleanor [Agronomy Department, Plant Molecul↗

Evaluation of flat-plate collector efficiency under controlled conditions in a solar simulator

The measured thermal efficiencies of 35 collectors tested with a solar simulator, along with the correlation equations used to generalize the data, are presented. The single correlation used is shown to apply to all the different types of collectors tested, including one with black paint and one cover, one with a selective surface coating and two covers, and an evacuated-tube collector. The test and correlation technique is also modified by using a shield so that collectors larger than the simulator test area can also be tested. This technique was verified experimentally for a shielded collector for which the collector shielded area was 31% of the solar simulator radiation area. A table lists all the collectors tested, the collector areas, and the experimental constants used to correlate the data for each collector.

Johnson, S. M.↗

Spectroscopic Needs for Imaging Dark Energy Experiments

Ongoing and near-future imaging-based dark energy experiments are critically dependent upon photometric redshifts (a.k.a. photo-z’s): i.e., estimates of the redshifts of objects based only on flux information obtained through broad filters. Higher-quality, lower-scatter photo-z’s will result in smaller random errors on cosmological parameters; while systematic errors in photometric redshift estimates, if not constrained, may dominate all other uncertainties from these experiments. The desired optimization and calibration is dependent upon spectroscopic measurements for secure redshift information; this is the key application of galaxy spectroscopy for imaging-based dark energy experiments. Hence, to achieve their full potential, imaging-based experiments will require large sets of objects with spectroscopically-determined redshifts, for two purposes: Training: Objects with known redshift are needed to map out the relationship between object color and z (or, equivalently, to determine empirically-calibrated templates describing the rest-frame spectra of the full range of galaxies, which may be used to predict the color-z relation). The ultimate goal of training is to minimize each moment of the distribution of differences between photometric redshift estimates and the true redshifts of objects, making the relationship between them as tight as possible. The larger and more complete our “training set” of spectroscopic redshifts is, the smaller the RMS photo-z errors should be, increasing the constraining power of imaging experiments; Requirements: Spectroscopic redshift measurements for ~30,000 objects over >~15 widely-separated regions, each at least ~20 arcmin in diameter, and reaching the faintest objects used in a given experiment, will likely be necessary if photometric redshifts are to be trained and calibrated with conventional techniques. Larger, more complete samples (i.e., with longer exposure times) can improve photo-z algorithms and reduce scatter further, enhancing the science return from planned experiments greatly (increasing the Dark Energy Task Force figure of merit by up to ~50%); Options: This spectroscopy will most efficiently be done by covering as much of the optical and near-infrared spectrum as possible at modestly high spectral resolution (λ/Δλ > ~3000), while maximizing the telescope collecting area, field of view on the sky, and multiplexing of simultaneous spectra. The most efficient instrument for this would likely be either the proposed GMACS/MANIFEST spectrograph for the Giant Magellan Telescope or the OPTIMOS spectrograph for the European Extremely Large Telescope, depending on actual properties when built. The PFS spectrograph at Subaru would be next best and available considerably earlier, c. 2018; the proposed ngCFHT and SSST telescopes would have similar capabilities but start later. Other key options, in order of increasing total time required, are the WFOS spectrograph at TMT, MOONS at the VLT, and DESI at the Mayall 4 m telescope (or the similar 4MOST and WEAVE projects); of these, only DESI, MOONS, and PFS are expected to be available before 2020. Table 2-3 of this white paper summarizes the observation time required at each facility for strawman training samples. To attain secure redshift measurements for a high fraction of targeted objects and cover the full redshift span of future experiments, additional near-infrared spectroscopy will also be required; this is best done from space, particularly with WFIRST-2.4 and JWST; Calibration: The first several moments of redshift distributions (the mean, RMS redshift dispersion, etc.), must be known to high accuracy for cosmological constraints not to be systematics-dominated (equivalently, the moments of the distribution of differences between photometric and true redshifts could be determined instead). The ultimate goal of calibration is to characterize these moments for every subsample used in analyses - i.e., to minimize the uncertainty in their mean redshift, RMS dispersion, etc. – rather than to make the moments themselves small. Calibration may be done with the same spectroscopic dataset used for training if that dataset is extremely high in redshift completeness (i.e., no populations of galaxies to be used in analyses are systematically missed). Accurate photo-z calibration is necessary for all imaging experiments; Requirements: If extremely low levels of systematic incompleteness (<~0.1%) are attained in training samples, the same datasets described above should be sufficient for calibration. However, existing deep spectroscopic surveys have failed to yield secure redshifts for 30–60% of targets, so that would require very large improvements over past experience. This incompleteness would be a limiting factor for training, but catastrophic for calibration. If <~0.1% incompleteness is not attainable, the best known option for calibration of photometric redshifts is to utilize cross-correlation statistics in some form. The most direct method for this uses cross-correlations between positions on the sky of bright objects of known spectroscopic redshift with the sample of objects that we wish to calibrate the redshift distribution for, measured as a function of spectroscopic z. For such a calibration, redshifts of ~100,000 objects over at least several hundred square degrees, spanning the full redshift range of the samples used for dark energy, would be necessary; and Options: The proposed BAO experiment eBOSS would provide sufficient spectroscopy for basic calibrations, particularly for ongoing and near-future imaging experiments. The planned DESI experiment would provide excellent calibration with redundant cross-checks, but will start after the conclusion of some imaging projects. An extension of DESI to the Southern hemisphere would provide the best possible calibration from cross-correlation methods for DES and LSST. We thus anticipate that our two primary needs for spectroscopy – training and calibration of photometric redshifts – will require two separate solutions. For ongoing and future projects to reach their full potential, new spectroscopic samples of faint objects will be needed for training; those new samples may be suitable for calibration, but the latter possibility is uncertain. In contrast, wide-area samples of bright objects are poorly suited for training, but can provide high-precision calibrations via cross-correlation techniques. Additional training/calibration redshifts and/or host galaxy spectroscopy would enhance the use of supernovae and galaxy clusters for cosmology. We also summarize additional work on photometric redshift techniques that will be needed to prepare for data from ongoing and future dark energy experiments.

79 ASTRONOMY AND ASTROPHYSICS↗

G4CEP: A G4 theory modification by including pseudopotential for molecules containing first-, second- and third-row representative elements

The G4CEP composite method was developed from the respective G4 all-electron version by considering the implementation of compact effective pseudopotential (CEP). The G3/05 test set was used as reference to benchmark the adaptation by treating in this work atoms and compounds from the first and second periods of the periodic table, as well as representative elements of the third period, comprising 440 thermochemical data. G4CEP has not reached a so high level of accuracy as the G4 all-electron theory. G4CEP presented a mean absolute error around 1.09 kcal mol{sup −1}, while the original method presents a deviation corresponding to 0.83 kcal mol{sup −1}. The similarity of the optimized molecular geometries between G4 and G4CEP indicates that the core-electron effects and basis set adjustments may be pointed out as a significant factor responsible for the large discrepancies between the pseudopotential results and the experimental data, or even that the all-electron calculations are more efficient either in its formulation or in the cancellation of errors. When the G4CEP mean absolute error (1.09 kcal mol{sup −1}) is compared to 1.29 kcal mol{sup −1} from G3CEP, it does not seem so efficient. However, while the G3CEP uncertainty is ±4.06 kcal mol{sup −1}, the G4CEP deviation is ±2.72 kcal mol{sup −1}. Therefore, the G4CEP theory is considerably more reliable than any previous combination of composite theory and pseudopotential, particularly for enthalpies of formation and electron affinities.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

FLUKA Analysis of Linac Fault Study Dose Rates

The planned Linac Fault Study was conducted on December 5 and 6, 2013 according to the Fault Study Procedure. Beam energy out the end of the Linac was typically 200 MeV, except for the LB-B1 Fault study that used 100 MeV beam out of the 4 th accelerator cavity (ACC4, also called and labeled as 4 th accelerating structure, AS4). The Linac fault study where beam was steered into the 4 th accelerator cavity (upstream) had beam energy of 150 MeV, since the last cavity was not traversed. Table I describes the list of the Beam Loss Fault Studies generated for verification of the Linac tunnel bulk and Linac supplemental shield effectiveness. The beam charge for the loss locations was typically measured at the LB-IT1 integrating current monitor after the ACC4 cavity, between LB-Q1 and LB-Q2. If beam was transported to the dumps a Faraday cup (LB-DU1 or LBDU2) monitor will yield the transported current to the dump, with the difference between LB-IT1 and LB-DU1 yielding the charge lost ahead of the dump. For beam miss-steered or intercepted ahead of the dumps the transport efficiency is zero. For the ACC4 beam loss fault the assumed current was the LB-ITI value before the miss-steering. For most of the faults the LB-ITI current was reading ~13.4 nC and the pulse rate was 1Hz, for 13.4 nC/s beam current.

36 MATERIALS SCIENCE↗

Performance of high spatial frequency X-ray transmission gratings

The performance of high spatial frequency 'phased' X-ray transmission gratings developed for the High Energy Transmission Grating Spectrometer on the Advanced X-ray Astrophysics FAcility (AXAF) is examined. The gratings tested here nominally consist of 1-micron-thick gold lines of 0.2 micron period covering approximately 5 sq cm of a polyimide membrane. A table-top setup at MIT employs the gratings in reflection to diffract UV (325 nm) laser light. It is used to measure grating periods and indicates that period variations within and between gratings are a few parts in 10,000. Tests performed at the Marshall Space Flight Center 304 m X-ray Facility using 1.5 keV X-rays in transmission corroborate the UV measurements and demonstrate geometrically-limited resolving powers of E/Delta E about 750. Finally, X-ray transmission tests performed in the MIT 25 m X-ray facility provide measurements of period, line thickness, space-to-period ratio, tilt of grating lines, and efficiency.

Fischbach, K. F.↗

Using Convex Optimization to Efficiently Apportion Tracer and Pollutant Sources From Point Concentration Observations

Abstract Rivers transport elements, minerals, chemicals, and pollutants produced in their upstream basins. A sample from a river is a mixture of all of its upstream sources, making it challenging to pinpoint the contribution from each individual source. Here, we show how a nested sample design and convex optimization can be used to efficiently unmix downstream samples of a well‐mixed, conservative tracer in a steady state system into the contributions of their upstream sources. Our approach is significantly faster than previous methods. We represent the river's sub‐catchments, defined by sampling sites, using a directed acyclic graph. This graph is used to build a convex optimization problem which, thanks to its convexity, can be quickly solved to global optimality—in under a second on desktop hardware for data sets of ∼100 samples or fewer. Uncertainties in the upstream predictions can be generated using Monte Carlo resampling. We provide an open‐source implementation of this approach in Python. The inputs required are straightforward: a table containing sample locations and observed tracer concentrations, along with a D8 flow‐direction raster map. As a case study, we use this method to map the elemental geochemistry of sediment sources for rivers draining the Cairngorms mountains, UK. This method could be extended to non‐conservative and non‐steady state tracers. We also show, theoretically, how multiple tracers could be simultaneously inverted to recover upstream run‐off or erosion rates as well as source concentrations. Overall, this approach can provide valuable insights to researchers in various fields, including water quality, geochemical exploration, geochemistry, hydrology, and wastewater epidemiology.

Barnes, Richard↗

Evaluation of flat-plate collector efficiency under controlled conditions in a solar simulator

The measured thermal efficiencies of 35 collectors tested with a solar simulator, along with the correlation equations used to generalize the data, are presented in this report. The single correlation used is shown to apply to all the different types of collectors tested, including one with black paint and one cover, one with a selective surface coating and two covers, and an evacuated-tube collector. The test and correlation technique is also modified by using a shield so that collectors larger than the simulator test area can also be tested. This technique was verified experimentally for a shielded collector for which the collector shielded area was 31% of the solar simulator radiation area. A table lists all the collectors tested, the collector areas, and the experimental constants used to correlate the data for each collector.

Johnson, S. M.↗

Machine learning elastic constants of multi-component alloys

The present manuscript explores application of machine learning methods for determining elastic constants and other derived mechanical properties of multi-component alloys. Here, a number of machine learning models, including linear regression, neural network and random forest based models, are trained and tested on a dataset of binary alloys generated using density functional theory (DFT) calculations and spanning over a large number of elemental species in the periodic table. Starting with a wide range of simple and easily accessible compositionally-averaged elemental features, a correlation-based feature selection strategy was used to systematically down-select a set of most relevant features towards the prediction of the elasticity tensor components. The true predictive performance and the associated uncertainties of the models were established by testing on unseen data and bootstrapping, respectively. A single and pair-wise feature partial dependence analysis was performed to visualize the average property trends in the multi-dimensional feature space in order to further understand the achieved predictive performance. The utility of the trained model is further demonstrated by obtaining sufficiently accurate yet highly efficient approximations for bulk modulus, Young’s modulus, shear modulus and Poisson’s ratio for alloys beyond the binary space (i.e., two-component alloys) on which the model was originally trained. More importantly, we test and validate the predictive performance of the developed model directly against the experimentally measured elastic constants of technologically relevant multi-component alloys (such as, Ni- and Ti-based alloys). Finally, utility of such a data-enabled route is demonstrated by predicting the possible range of various elastic properties for vast composition space available within the five component Ni-Cr-Fe-Mo-W alloy system in a high-throughput manner.

36 MATERIALS SCIENCE↗

Engineering Ralstonia eutropha for Production of Isobutanol (IBT) Motor Fuel from Carbon Dioxide, Hydrogen, and Oxygen Project Final Report

This research project is a collaboration between the Sinskey laboratory at MIT and the Worden laboratory at Michigan State University. The goal of the project is to produce Isobutanol (IBT), a branched-chain alcohol that can serve as a drop-in transportation fuel, through the engineered microbial biosynthesis of Carbon Dioxide, Hydrogen, and Oxygen using a novel bioreactor. This final technical report presents the findings of both the biological engineering work at MIT that extended the native branched-chain amino acid pathway of the wild type Ralstonia eutropha H16 to perform this biosynthesis, as well as the unique design, modeling, and construction of a bioreactor for incompatible gasses at Michigan State that enabled the operational testing of the complete system. This 105 page technical report summarizing the three years of research includes 72 figures and 11 tables of findings. Ralstonia eutropha (also known as Cupriavidus necator) is a Gram-negative, facultatively chemolithoautotrophic bacteria. It has been the principle organism used for the study of polyhydroxybutyrate (PHB) polymer biosynthesis. The wild-type Ralstonia eutropha H16 produces PHB as an intracellular carbon storage material while under nutrient stress in the presence of excess carbon. Under this stress, it can accumulate approximately 80 % of its cell dry weight (CDW) as this intracellular polymer. With the restoration of the required nutrients, the cells are then able to catabolize this polymer. If extracted from the cell, this PHB polymer can be processed into biodegradable and biocompatible plastics, however for this research, it is the efficient metabolic pathway channeling the captured carbon that is of interest. R. eutropha is further unique in that it contains two carbon-fixation Calvin–Benson–Bassham cycle operons, two oxygen-tolerant hydrogenases, and several formate dehydrogenases. It has also been much studied for its ability in the presence of oxygen, to fix carbon dioxide into complex cellular molecules using the energy from hydrogen. In this research project, engineered strains of R. eutropha redirected the excess carbon from PHB storage into the production of isobutanol and 3-methyl-1-butanol (branched-chain higher alcohols). These branched-chain higher alcohols can be used directly as substitutes for fossil-based fuels and are seen as alternative biofuels to ethanol and biodiesel. Importantly, these alcohols have approximately 98 % of the energy content of gasoline, 17 % higher than the current gasoline additive ethanol, without impacting corn market production for feed or food. Unlike ethanol, these branched-chain alcohols have low vapor pressure, hygroscopicity, and water solubility, which make them readily compatible with the existing pipelines, gasoline pumps, and engines in our transportation infrastructure. While the use of alternative energies from solar, wind, geothermal, and hydroelectric has spread for stationary power applications, these energy sources cannot be effectively or efficiently employed in current or future transportation systems. With the ongoing concerns of fossil fuel availability and price stability over the long term, alternative biofuels like branched-chain higher alcohols hold promise as a suitable transportation fuel in the future. We showed in our research that various mutant strains of R. eutropha with isobutyraldehyde dehydrogenase activity, in combination with the overexpression of plasmid-borne, native branched-chain amino acid biosynthesis pathway genes and the overexpression of heterologous ketoisovalerate decarboxylase gene, would produce isobutanol and 3-methyl-1-butanol when initiated during nitrogen or phosphorus limitation. Early on, we isolated one mutant R. eutropha strain which produced over 180 mg/L branched-chain alcohols in flask culture while being more tolerant of isobutanol toxicity. After the targeted elimination of genes encoding several potential carbon sinks (ilvE, bkdAB, and aceE), the production titer of the improved to 270 mg/L isobutanol and 40 mg/L 3-methyl-1-butanol. Semicontinuous flask cultivation supplied the cells with sufficient nutrients while minimizing the toxicity caused by isobutanol. Under this cultivation, the R. eutropha mutant grew and produced more than 14 g/L branched-chain alcohols over the duration of 50 days. These results demonstrate that R. eutropha carbon flux can be redirected from PHB to branched-chain alcohols and that engineered R. eutropha can be cultivated over prolonged periods of time for product biosynthesis. While this bioengineering work was being done at the Sinskey laboratory at MIT, the researchers at the Worden laboratory at Michigan State were working on the design and construction of the required specialty bioreactor for incompatible gasses (BIG) that would allow the safe feeding of microbes on Carbon Dioxide, Hydrogen, and Oxygen without explosive results. The early design and assembly work in year 1 incorporated a novel microbubble generator to maximize the bioavailability of gasses within the system comprised of small scale hollow fiber reactors. The early success of the microbubble generator eliminated the need to investigate potentially toxic surfactants within the system. For operational control, the system design incorporated a Opto22-based control network. The researchers also selected the specific hollow fiber material suitable for the bioreactor application. A variety of commercially available hollow fiber membranes were compared with regard to their pore sizes, cell affinity, and potential interference to cell viability assays. The selected membrane with its spongy layer was then tested for diffusivity of O2 and CO2. The instrumented system was then fully assembled for experimentally measuring the heterotrophic growth rate of immobilized R. eutropha cells. The requisite procedures for inoculation, measurement, and cleaning were established enabling the system performance to be validated under controlled laboratory conditions. In year 2, the researchers completed the Opto22 based cross-platform control network, and the system’s communications across the Sartorius fermentation system and Bruker gas chromatograph was established via open platform communications (OPC) protocol. Using the revised system, measurements were taken of the R.eutropha cell growth rate and substrate mass transfer rate in the hollow fiber membrane. Several IBT recovery strategies were explored and resin adsorption was determined to be optimal solution for lab scale operations. The adsorption capacity of the resin column was then measured and IBT desorption using methanol has been demonstrated. With the growing body of experimental data in hand, mathematical models were constructed to demonstrate and map the cellular kinetics, mass transfer of heterotrophic and autotrophic substrates in the hollow fiber, and the adsorption process in the resin column. A structured kinetic model was constructed to describe the competition between cell mass generation and IBT production. The reactor was then scaled up from single fiber to a membrane area of 180 cm2 and then further to 1 ft2. In Year 3 of the research, the IBT mass transfer across the membrane was characterized within the system with experiments to empirically measure the IBT diffusion coefficient in the BIG spongy layer. Using the refined mathematical models, the researchers are now able to explain the experimental observations and predict bioreactor performance under a wide range of experimental conditions. The Big system is able to demonstrate continuous controlled operations with the integrated IBT recovery system. Both heterotrophic and autotrophic production have been shown during continuous operation with heterotrophic and autotrophic stages. Performance of BIG system has been measured during continuous run with alternating heterotrophic growth on fructose and autotrophic product formation on H2, CO2, and O2. Volumetric productivities of IBT at 325 mg/(L day) and of 3M1B at 50 mg/(L day) were achieved, which were comparable to that achieved under heterotrophic conditions. Using the mathematical model, researchers are able to predict system performance for scaled-up BIG system. The apparent diffusion coefficient of IBT in the spongy layer of XM-50 hollow fiber membranes has been measured at various lumen liquid flow rates. The experiment is simulated in COMSOL to validate the results. The constructed COMSOL model is able to simulate BIG system performance in both batch and continuous mode. Mathematical simulations of the system performance have been run to identify the most crucial operational conditions, identifying the rate-limiting factors in autotrophic production of IBT, and quantitating the rate of IBT catabolism. Investigations of the productivity of the production system have suggested and the modeling of the system has revealed a particular sensitivity to the catabolism of the produced IBT by the engineered R. eutropha. Experiments have been designed and executed to quantify the IBT catabolism of R. eutropha, which open up possibilities for further system improvements through future, targeted bioengineering of the strain. Finally, the researchers at Michigan State performed an economic analysis of the system, based on the collective results, and their findings are presented in full within the report.

08 HYDROGEN↗

Performance of High Temperature Operational Amplifier, Type LM2904WH, under Extreme Temperatures

Operation of electronic parts and circuits under extreme temperatures is anticipated in NASA space exploration missions as well as terrestrial applications. Exposure of electronics to extreme temperatures and wide-range thermal swings greatly affects their performance via induced changes in the semiconductor material properties, packaging and interconnects, or due to incompatibility issues between interfaces that result from thermal expansion/contraction mismatch. Electronics that are designed to withstand operation and perform efficiently in extreme temperatures would mitigate risks for failure due to thermal stresses and, therefore, improve system reliability. In addition, they contribute to reducing system size and weight, simplifying its design, and reducing development cost through the elimination of otherwise required thermal control elements for proper ambient operation. A large DC voltage gain (100 dB) operational amplifier with a maximum junction temperature of 150 C was recently introduced by STMicroelectronics [1]. This LM2904WH chip comes in a plastic package and is designed specifically for automotive and industrial control systems. It operates from a single power supply over a wide range of voltages, and it consists of two independent, high gain, internally frequency compensated operational amplifiers. Table I shows some of the device manufacturer s specifications.

Patterson, Richard↗

Support of US CLIVAR Project Office 2012

Director of JOSS, supervised the U.S. CLIVAR Project Office Director and helped direct the officer to enhance the goals and objectives of the U.S. CLIVAR Project and budget. Financial Manager of JOSS, worked to complete proposals and monitor compliance with award requirements and funding limitations and ensure the U.S. CLIVAR Project Office complied with UCAR policies and procedures. Project Coordinator administered the funding for the U.S. CLIVAR Project Office and was responsible for coordinating special projects that required additional support from JOSS technical staff. These projects included activities such as website updates, technology upgrades, production of printed reports, and development of graphic elements like logos. Web Developer worked both on web development and graphic work and the work consisted of the following: Maintaining the site ? installing updates to Drupal CMS (Content Management System). Creating new templates for webpages and styling them with CSS and JavaScript/jQuery code. Fixing the styling on webpages that the content contributor/manager (Jenn Mays) created and has had trouble with. Creating new web forms for abstract uploading, subscriptions, and meeting registrations. Created 4 webpages for the ?ASP: Key Uncertainties in the Global Carbon-Cycle? meeting. Developed a document review form, instruction webpages, login redirect, dynamic table with form submissions for the US CLIVAR SSC Science Plan Document Review. This was open to the public from June 12, 2013 until July 10, 2013. During this time the user accounts had to be checked (daily) that were created by the public, to delete any spam ones. Graphics work: preparing images for general use on webpages, webpage banners, and for meeting name badges, creating a US CLIVAR letterhead, redesigning the US AMOC logo. System Administrator spent time working on the migration of the US CLIVAR site from the USGCRP office to UCAR here Boulder. This was done to increase the general speed of the site & to allow the web developer to work in it more efficiently. Main tasks were to Archive the old Site, create new development site for web developer, and move web address to new website when web developer was finished with development. There are no patients or equipment related to this proposals

99 GENERAL AND MISCELLANEOUS↗

A novel calibration method for non-orthogonal shaft laser theodolite measurement system

Non-orthogonal shaft laser theodolite (N-theodolite) is a new kind of large-scale metrological instrument made up by two rotary tables and one collimated laser. There are three axes for an N-theodolite. According to naming conventions in traditional theodolite, rotary axes of two rotary tables are called as horizontal axis and vertical axis, respectively, and the collimated laser beam is named as sight axis. And the difference between N-theodolite and traditional theodolite is obvious, since the former one with no orthogonal and intersecting accuracy requirements. So the calibration method for traditional theodolite is no longer suitable for N-theodolite, while the calibration method applied currently is really complicated. Thus this paper introduces a novel calibration method for non-orthogonal shaft laser theodolite measurement system to simplify the procedure and to improve the calibration accuracy. A simple two-step process, calibration for intrinsic parameters and for extrinsic parameters, is proposed by the novel method. And experiments have shown its efficiency and accuracy.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

SU-D-BRD-01: An Automated Physics Weekly Chart Checking System Supporting ARIA

Purpose: A software tool was developed in this study to perform automatic weekly physics chart check on the patient data in ARIA. The tool accesses the electronic patient data directly from ARIA server and checks the accuracy of treatment deliveries, and generates reports which summarize the delivery history and highlight the errors. Methods: The tool has four modules. 1) The database interface is designed to directly access treatment delivery data from the ARIA database before reorganizing the data into the patient chart tree (PCT). 2) PCT is a core data structure designed to store and organize the data in logical hierarchies, and to be passed among functions. 3) The treatment data check module analyzes the organized data in PCT and stores the checking results into PCT. 4) Report generation module generates reports containing the treatment delivery summary, chart checking results and plots of daily treatment setup parameters (couch table positions, shifts of image guidance). The errors that are found by the tool are highlighted with colors. Results: The weekly check tool has been implemented in MATLAB and clinically tested at two major cancer centers. Javascript, cascading style sheets (CSS) and dynamic HTML were employed to create the user-interactive reports. It takes 0.06 second to search the delivery records of one beam with PCT and compare the delivery records with beam plan. The reports, saved in the HTML files on shared network folder, can be accessed by web browser on computers and mobile devices. Conclusion: The presented weekly check tool is useful to check the electronic patient treatment data in Varian ARIA system. It could be more efficient and reliable than the manually check by physicists. The work was partially supported by a research grant from Varian Medical System.

60 APPLIED LIFE SCIENCES↗

Map reduce using coordination namespace hardware acceleration

A system and method for supporting data MapReduce operations in a tuple space/coordinated namespace (CNS) extended memory storage architecture. The system-wide CNS provides an efficient means for storing and communicating data generated by local processes running at the nodes, and coordinated to provide MapReduce operations in a multi-nodal system. A hardware accelerated mechanism supports map reduce sorting/shuffle operations and reduce operations according to an aggregate function. Local processes running at a node generate a tuple corresponding to data generated by a process, each tuple having a tuple name and tuple data value corresponding to the generated data. Each tuple is processed and stored at the node or another node, dependent upon its tuple name. Tuple records associated with a tuple name are accumulated at one or more nodes according to a linked list structure at each that is accessible via a hash table index pointer at the node.

Jacob, Philip↗

Web Exploration Tools for a Fast Federated Optical Survey Database

We implemented several new web-based tools to improve the efficiency and versatility of access to the APS Catalog of the POSS I (Palomar Observatory-National Geographic Sky Survey) and its associated image database. The most important addition was a federated database system to link the APS Catalog and image database into one Internet-accessible database. With the FDBS, the queries and transactions on the integrated database are performed as if it were a single database. We installed Myriad the FDBS developed by Professor Jaideep Srivastava and members of his group in the University of Minnesota Computer Science Department. It is the first system to provide schema integration, query processing and optimization, and transaction management capabilities in a single framework. The attached figure illustrates the Myriad architecture. The FDBS permits horizontal access to the data, not just vertical. For example, for the APS, queries can be made not only by sky position, but also by any parameter present in either of the databases. APS users will be able to produce an image of all the blue galaxies and stellar sources for comparison with x-ray source error ellipses from AXAF (X Ray Astrophysics Facility) (Chandra) for example. The FDBS is now available as a beta release with the appropriate query forms at our web site. While much of our time was occupied with adapting Myriad to the APS environment, we also made major changes in Star Base, our DBMS for the Catalog, at the web interface to improve its efficiency for issuing and processing queries. Star Base is now three times faster for large queries. Improvements were also made at the web end of the image database for faster access; although work still needs to be done to the image database itself for more efficient return with the FDBS. During the past few years, we made several improvements to the database pipeline that creates the individual plate databases queries by StarBase. The changes include improved positions especially for galaxies, using a new median centroider and integrated magnitudes for galaxies with an improved density-to-intensity calibration with a "sky" background subtraction. In the original version of StarBase the object classification fainter than 19.5-20.0 mag., was an extrapolation of the networks trained on brighter objects. We have used a new catalog of galaxies at the NGP to train a neural network on objects fainter than 20th mag. This improved classification is used in the new version of StarBase. We have also added a FITS table option for the returned data from queries on the object catalog. The APS image database includes images in both colors so we have added a tool for querying the image database in both colors simultaneously. The images can be displayed in parallel or blinked for comparison.

Humphreys, Roberta M.↗