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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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NASA NTRSDate not supplied

Chemical Reactivity of In-Situ Lunar Dust for Biotoxicity Assessment

Introduction: How does the chemical reactivity of in-situ lunar dust compare to Apollo samples currently stored in curation facilities here on Earth? Essential investigations of this question will help us to further mitigate exploration risks for future human explorers on the Moon and will also provide critical information for astrobiologists and space biologists using the Moon for scientific inquiry. Discussion: Apollo 14 dust biotoxicity studies, carried out by the NASA Lunar Airborne Dust Toxici-ty Assessment Group (LADTAG), included numerous physiochemical studies[1] and cellular and animal ex-periments. Intratracheal instillation [2] and inhalation studies [3] in rats both showed Apollo 14 dust to be intermediate in toxicity compared to low-tox titanium dusts and high-tox quartz dusts of similar particle siz-es. The collective results were used in models [4] to establish a safe exposure limit for astronauts [5]. Alt-hough LADTAG took extensive steps to preserve what chemical reactivity may still have existed in the sam-ples, it is simply unknown if they possessed true in-situ chemical reactivity or if that reactivity has de-cayed. Initial gas loss on collection and other altera-tions, and even intermittent exposure to Earth-normal conditions during subsequent decades of handling, obscure a forensic reconstruction of the initial state. Because a mineral dust’s chemical reactivity influ-ences its biotoxicity [6], researchers have developed methods to “activate” lunar dust and simulants [7][8]. Past studies that modeled impact processes and radia-tion [9] in the lunar environment suggest that in-situ lunar dust is likely to be more chemically reactive than Earth-exposed samples. Because of these results, in-situ measurements are warranted [10]. Other studies have examined the hydroxyl generating capability of iron bearing mineral phases [11][12] and further em-phasize the role iron plays in chemical reactivity of lunar material, as well as decay of chemical reactivity in mineral dusts [12]. Recent observations of the lunar surface reveal the presence of hematite [13], a finding that further supports the hypothesis that in-situ lunar dust is reactive. Since the lunar surface is heterogene-ous, dust biotoxicity is expected to vary from site to site [14] due to particle size, mineralogy, physical characteristics, degree of space weathering, and chemi-cal reactivity (Figure 1). This circumstance dictates dust assessments at a suite of lunar sites enabled by upcoming NASA and commercial lunar payload ser-vices (CLPS) opportunities. Dose, location, and dura-tion of particle exposure will also affect biological responses. In-situ chemical reactivity measurements can inform cross-cutting collaborative research cam-paigns such as astrobiology studies examining regolith interactions with organisms and its ability to preserve chemical and structural biomarkers, as well as space biology investigations that examine regolith-microbe interactions relating to life support systems, plant growth, biomining, and development of regolith bio-composites. Figure 1: Environment conditions on the lunar surface that may alter regolith reactivity. Summary A series of in-situ measurements of lu-nar dust free radical chemistry at future Artemis and CLPS landing sites, combined with LADTAG-like studies of freshly collected lunar dust specimens, will reveal the true chemical reactivity of in-situ lunar dust and generate scientific data that can be compared to the chemical reactivity and biotoxicity of samples from Apollo landing sites. Furthermore, results from in situ measurements and biotoxicity studies of freshly col-lected specimens can also be used to validate, or re-quire revision of, the current astronaut permissible exposure limit [15]. References: [1] McKay D et al (2015), Acta As-tronaut 107:163–176. [2] Rask J et al (2013), LPSC, p 3062. [3] Lam CW et al (2013), Inhal Toxicol 25:661–678. [4] James JT, et. al. (2013) , Inhal Toxicol 25:243–256. [5] Scully RR, et.al. (2013), Inhal Toxi-col 25:785–793. [6] Porter, D. W., et.al., (2002), Tox-icology 175, 63–71. [7] Wallace WT, et.al., (2009), Meteorit Planet Sci 44:961–970. [8] Wallace WT, et.al., (2010), Earth Planet Sci Lett 295:571–577. [9] Loftus D, Rask J, et.al., (2010), Earth Moon Planet 107:95–105. [10] Rask J, et.al., (2009) LEAG p 57. [11] Turci F, et.a., (2015), Astrobiology. 2015;15(5):371-380. [12] Hendrix DA, et.al., (2019), Geohealth. 2019;3(1):28-42. [13] Li, S., et.al., (2020), Science advances, 6(36), p.eaba1940. [14] Rask J. (2018), In: Cudnik B. (eds) Encyclopedia of Lunar Science. Springer, Cham. [15] Rask, J, (2020), LPI, Artemis III Sci. def. paper 2120.

chemical reactivity↗
NASA NTRS1977

DESAP 1: A structural design program with stress and displacement constraints. Volume 1: Theoretical and user's manual

A finite element program is presented for computer-automated, minimum weight design of elastic structures with constraints on stresses (including local instability criteria) and displacements. Volume 1 of the report contains the theoretical and user's manual of the program. Sample problems and the listing of the program are included in Volumes 2 and 3. The element subroutines are organized so as to facilitate additions and changes by the user. As a result, a relatively minor programming effort would be required to make DESAP 1 into a special purpose program to handle the user's specific design requirements and failure criteria.

Kiusalaas, J.↗
NASA NTRS1996

Fungal colonization of fiberglass insulation in the air distribution system of a multi-story office building: VOC production and possible relationship to a sick building syndrome

Complaints characteristic of those for sick building syndrome prompted mycological investigations of a modern multi-story office building on the Gulf coast in the Southeastern United States (Houston-Galveston area). The air handling units and fiberglass duct liner of the heating, ventilating and air conditioning system of the building, without a history of catastrophic or chronic water damage, demonstrated extensive colonization with Penicillium spp and Cladosporium herbarum. Although dense fungal growth was observed on surfaces within the heating-cooling system, most air samples yielded fewer than 200 CFU m-3. Several volatile compounds found in the building air were released also from colonized fiberglass. Removal of colonized insulation from the floor receiving the majority of complaints of mouldy air and continuous operation of the units supplying this floor resulted in a reduction in the number of complaints.

NASA Program Environmental Health↗
NASA NTRS2014

A User Guide for Smoothing Air Traffic Radar Data

Matlab software was written to provide smoothing of radar tracking data to simulate ADS-B (Automatic Dependent Surveillance-Broadcast) data in order to test a tactical conflict probe. The probe, called TSAFE (Tactical Separation-Assured Flight Environment), is designed to handle air-traffic conflicts left undetected or unresolved when loss-of-separation is predicted to occur within approximately two minutes. The data stream that is down-linked from an aircraft equipped with an ADS-B system would include accurate GPS-derived position and velocity information at sample rates of 1 Hz. Nation-wide ADS-B equipage (mandated by 2020) should improve surveillance accuracy and TSAFE performance. Currently, position data are provided by Center radar (nominal 12-sec samples) and Terminal radar (nominal 4.8-sec samples). Aircraft ground speed and ground track are estimated using real-time filtering, causing lags up to 60 sec, compromising performance of a tactical resolution tool. Offline smoothing of radar data reduces wild-point errors, provides a sample rate as high as 1 Hz, and yields more accurate and lag-free estimates of ground speed, ground track, and climb rate. Until full ADS-B implementation is available, smoothed radar data should provide reasonable track estimates for testing TSAFE in an ADS-B-like environment. An example illustrates the smoothing of radar data and shows a comparison of smoothed-radar and ADS-B tracking. This document is intended to serve as a guide for using the smoothing software.

Center/Tracon Radar↗
NASA NTRSDate not supplied

Wildfire Segmentation From Remotely Sensed Data Using Quantum-Compatible Conditional Vector Quantized-Variational Autoencoders

Wildfires represent a critical environmental hazard with multifaceted implications for ecosystems, communities, and public health [1]. The escalating frequency and intensity of wildfires globally have intensified the urgency for robust segmentation methodologies to facilitate effective mitigation, response, and recovery strategies [2]. Accurate wildfire segmentation is pivotal for delineating fire boundaries, assessing progression patterns, and prioritizing resource allocation during emergency scenarios. Furthermore, precise segmentation enables stakeholders, including policymakers, environmental scientists, and emergency responders, to formulate evidence-based strategies, thereby minimizing socio-economic disruptions and ecological degradation. Consequently, advancing wildfire segmentation techniques through innovative technological interventions remains a paramount research imperative. Although foundational in wildfire segmentation, traditional deterministic models exhibit inherent limitations that compromise their efficacy in dynamic and uncertain environments. These models often operate on rigid algorithms prioritizing deterministic classifications, thereby overlooking the inherent complexities and uncertainties associated with wildfire behavior and satellite data variability. Such deterministic frameworks tend to produce oversimplified representations that fail to capture the intricate nuances of evolving fire dynamics, spatial heterogeneity, and environmental interactions [1]. Consequently, the deterministic approach’s propensity for uncertainty collapsing [1, 3] hampers the accuracy, reliability, and applicability of segmentation outcomes in real-world scenarios. Contrastingly, stochastic models offer a more nuanced and adaptable framework for wildfire segmentation. By integrating probabilistic elements into the modeling paradigm, stochastic approaches, particularly probabilistic approaches such as variational auto encoders (VAEs) [4], facilitate comprehensive uncertainty assessment, enabling researchers to quantify and incorporate uncertainties into segmentation outcomes effectively. This probabilistic nature empowers stochastic models to encapsulate variability, account for data inconsistencies, and adapt to evolving environmental conditions, enhancing segmentation accuracy, reliability, and robustness. Embracing stochastic methodologies thus catalyzes advancements in wildfire science by fostering a more holistic, adaptive, and resilient segmentation framework. Despite VAEs demonstrating significant promise in various applications, they come with inherent limitations that have garnered attention within the machine learning community. One of the primary drawbacks lies in their reliance on static priors, which essentially assume a fixed distribution for latent variables, thereby limiting the model’s flexibility to capture complex data structures effectively [5]. This static nature leads to suboptimal representations, especially when dealing with complex and high-dimensional data. Additionally, VAEs often struggle with generating sharp and realistic samples, a phenomenon commonly referred to as mode collapse [5, 7, 6]. Furthermore, the optimization process in VAEs, which involves balancing the reconstruction loss and the regularization term, can sometimes be challenging to fine-tune [7]. In recent efforts to address these shortcomings, alternative approaches like Vector Quantized Variational Auto encoders(VQ-VAEs) [7], address the challenges by incorporating discrete latent variables and leveraging techniques that enhance the quality and diversity of generated samples while maintaining efficient training dynamics. VQ-VAEs propose a dynamic prior distribution generation mechanism that diverges from the static priors commonly associated with traditional VAEs. This dynamic approach allows for more adaptive and context-aware latent variable representations, thereby potentially capturing complex data structures more effectively. Unlike autoregressive prior models such as PixelCNN, which, despite their ability to model dependencies across data dimensions, suffer from significant computational inefficiencies and lack flexibility in handling diverse datasets. In our work, we propose to use a generative quantum-compatible approach to help alleviate the shortcomings of autoregressive prior model in VQ-VAEs. Restricted Boltzmann Machines (RBMs) are a viable alternative prior model that can learn prior distributions in a faster and more flexible manner. In this research endeavor, we meticulously curate a state-of-the-art dataset leveraging satellite MODIS data in conjunction with VIIRS fire masks, derived from Fire Radiative Power (FRP), thereby encapsulating diverse wildfire scenarios and environmental contexts. We developed a conditional VQ-VAE architecture with the RBM prior model that is trained in a supervised manner for segmenting wildfire masks. This innovative approach synergistically harnesses deep learning capabilities, enabling the generation of segmentation maps characterized by heightened precision, granularity, and contextual relevance. Furthermore, replacing the autoregressive prior learning method proposed by the original VQ-VAE with a prior density approximation via quantum-compatible RBM facilitates expedited inference processes, augments flexibility in prior sampling, optimizes computational efficiency and establishes a groundbreaking benchmark in wildfire segmentation methodologies.

quantum machine learning↗
NASA NTRS2001

Detection and Characterization of Imperfections in Composite Pressure Vessels

The thermographic evaluation of composite structures for delaminations, disbonds, inclusions, porosity and microcracking has proven to be a valuable asset in the field of nondestructive testing. Coupling large area coverage, variable sensitivity and minimal surface contact with a photographic type image representation of structural anomalies; thermography has become a primary inspection method for composite structures. Thermography works well for locating both surface and subsurface defects in most composite systems ranging in thickness of up to 0.25 inch (0.64 cm) or more. The thermographic method for inspection of composite structures typically involves applying an external source of heat to the structure and then recording the changes in the surface heat profile manifested by embedded defects or by material property variations. If these temperature variations are large enough and an infrared camera with sufficient sensitivity is used, then the material or structural abnormality can be detected and referred back to its source. Interpreting the information given in a thermogram, can be a difficult task under ideal circumstances and extremely challenging in a real world setting. Variables including depth, thermal conductivity, orientation and size of the abnormality can all have a great influence on how its heat pattern will be seen by the imager. The work discussed in this paper illustrates how the microstructure of several commonly found defects in composite structures relate to their thermographic image counterpart. Two test cases are studied herein, including a large graphite/epoxy RP-1 fuel tank and a graphite composite cryogenic fuel feedline. The structures used in this study were designed to serve as manufacturing proof of concept specimens and to undergo hydroburst testing to verify manufacturing practices and structural design. Prior to hydrostatic testing the structures underwent 100% thermographic evaluation to ensure that no manufacturing or handling damage was present. Due to a large inclusion found in the feedline, it was pulled from service and dissected without performing the hydroproof The thermographic indications found in the RP-1 tank were below critical limits so it underwent a complete hydroproof loading series and finally a hydroburst. Following the hydroburst samples were cut from the tank in regions where thermography had located damage before the test. These regions were thermographically and then were cross-sectioned and photomicrographed.

Walker, James L.↗
NASA NTRS2004

Plasma Interactions With a Negative Biased Electrodynamic Tether

The ProSEDS conductive tether design incorporates two distinct types of tethers from a plasma interaction viewpoint. The 200 m closest to the Delta II spacecraft is insulated from the plasma, and the remaining 4800 m is semi-bare. This latter portion is considered semi-bare because a conductive coating, which is designed to collect electrons from the plasma, was applied to the wires to regulate the overall tether temperature. Because the tether has both insulating and conductive tether sections, a transition point exists between the two that forms a triple point with the space plasma. Also, insulated tethers can arc to the space plasma if the insulation is weakened or breached by pinholes caused by either improper handling or small meteoroid and orbital debris strikes. Because electrodynamic tethers are typically long, they have a high probability of these impacts. The particles, which strike the tether, may not have sufficient size to severe the tether, but they can easily penetrate the tether insulation producing a plasma discharge to the ambient plasma. Samples of both the ProSEDS tether transition region and the insulated tether section with various size of pinholes were placed into the MSFC plasma chamber and biased to typical ProSEDS open circuit tether potentials (-500 V to -1600 V). The results of the testing showed that the transition region of the tether (i.e. the triple point) arced to the ambient plasma at -900 V, and the tethers damaged by a pinhole or simulated debris strike arced to the plasma between -700 V and -900 V. Specific design steps were taken to eliminate the triple point issue in the ProSEDS tether design and make it ready for flight. To reduce the pinhole arcing risk, ProSEDS mission operations were changed to eliminate the high negative potential on the insulated tether. The results of the testing campaign and the design changes implemented to ensure a successful flight are described.

Vaughn, Jason A.↗
NASA NTRS1997

Robotic Materials Handling in Space: Mechanical Design of the Robot Operated Materials Processing System HitchHiker Experiment

The Goddard Space Flight Center has developed the Robot Operated Materials Processing System (ROMPS) that flew aboard STS-64 in September, 1994. The ROMPS robot transported pallets containing wafers of different materials from their storage racks to a furnace for thermal processing. A system of tapered guides and compliant springs was designed to deal with the potential misalignments. The robot and all the sample pallets were locked down for launch and landing. The design of the passive lockdown system, and the interplay between it and the alignment system are presented.

Voellmer, George↗
NASA NTRS1988

A multistage mesh generator for solving the average-passage equation system

One means of numerically simulating the 3-D flow field within a multistage turbomachine is through the solution of the average-passage equation system. One requirement of a current algorithm used to solve this system of equations has been the ability to generate multiple blade row meshes which satisfy specific geometrical constraints. In addition to meeting this criterion, one desires a mesh generation code which requires minimal user input, utilizes variable mesh control parameters, generates diagnostics helpful to the user, and possesses the capability to handle widely varying geometries. A mesh generation code with these features was written and has been used in solving the inviscid form of the average-passing equation system for both ducted and unducted multiple blade row geometries. This paper serves as a user reference guide, with a description of the mesh generation algorithm, a sample input file, and examples of typical meshes generated.

Mulac, Richard A.↗
NASA NTRS1986

High frequency power distribution system

The objective of this project was to provide the technology of high frequency, high power transmission lines to the 100 kW power range at 20 kHz frequency. In addition to the necessary design studies, a 150 m long, 600 V, 60 A transmission line was built, tested and delivered for full vacuum tests. The configuration analysis on five alternative configurations resulted in the final selection of the three parallel Litz straps configuration, which gave a virtually concentric design in the electromagnetic sense. Low inductance, low EMI and flexibility in handling are the key features of this configuration. The final design was made after a parametric study to minimize the losses, weight and inductance. The construction of the cable was completed with no major difficulties. The R,L,C parameters measured on the cable agreed well with the calculated values. The corona tests on insulation samples showed a safety factor of 3.

Patel, Mikund R.↗
NASA NTRS2010

Miniature Bioreactor System for Long-Term Cell Culture

A prototype miniature bioreactor system is designed to serve as a laboratory benchtop cell-culturing system that minimizes the need for relatively expensive equipment and reagents and can be operated under computer control, thereby reducing the time and effort required of human investigators and reducing uncertainty in results. The system includes a bioreactor, a fluid-handling subsystem, a chamber wherein the bioreactor is maintained in a controlled atmosphere at a controlled temperature, and associated control subsystems. The system can be used to culture both anchorage-dependent and suspension cells, which can be either prokaryotic or eukaryotic. Cells can be cultured for extended periods of time in this system, and samples of cells can be extracted and analyzed at specified intervals. By integrating this system with one or more microanalytical instrument(s), one can construct a complete automated analytical system that can be tailored to perform one or more of a large variety of assays.

Gonda, Steve R.↗
DOE OSTI2025

On the Abuse and Detection of Polyglot Files

A polyglot is a file that is valid in two or more formats. Polyglot files pose a problem for file-upload and generative AI web interfaces that rely on format identification to determine how to securely handle incoming files. In this work we found that existing file-format and embedded-file detection tools, even those developed specifically for polyglot files, fail to reliably detect polyglot files used in the wild. To address this issue, we studied the use of polyglot files by malicious actors in the wild, finding 30 polyglot samples and 15 attack chains that leveraged polyglot files. Using knowledge from our survey of polyglot usage in the wild---the first of its kind---we created a novel data set based on adversary techniques. We then trained a machine learning detection solution, PolyConv, using this data set. PolyConv achieves a precision-recall area-under-curve score of 0.999 with an F1 score of 99.20% for polyglot detection and 99.47% for file-format identification, significantly outperforming all other tools tested. We developed a content disarmament and reconstruction tool, ImSan, that successfully sanitized 100% of the tested image-based polyglots, which were the most common type found via the survey. Our work provides concrete tools and suggestions to enable defenders to better defend themselves against polyglot files, as well as directions for future work to create more robust file specifications and methods of disarmament.

Oesch, T [ORNL] (ORCID:0000000269091022)↗
NASA NTRS1986

Method for the prediction of the installation aerodynamics of a propfan at subsonic speeds: User manual

This document is the user's guide for the method developed earlier for predicting the slipstream wing interaction at subsonic speeds. The analysis involves a subsonic panel code (HESS code) modified to handle the propeller onset flow. The propfan slipstream effects are superimposed on the normal flow boundary condition and are applied over the surface washed by the slipstream. The effects of the propeller slipstream are to increase the axial induced velocity, tangential velocity, and a total pressure rise in the wake of the propeller. Principles based on blade performance theory, momentum theory, and vortex theory were used to evaluate the slipstream effects. The code can be applied to any arbitrary three dimensional geometry, expressed in the form of HESS input format. The code can handle a propeller alone configuration or a propeller/nacelle/airframe configuration, operating up to high subcritical Mach numbers over a range of angles of attack. Inclusion of a viscous modelling is briefly outlined. Wind tunnel results/theory comparisons are included as examples for the application of the code to a generic supercritical wing/overwing Nacelle with a powered propfan. A sample input/output listing is provided.

Chandrasekaran, B.↗
NASA NTRS1990

Managing temporal relations

Various temporal constraints on the execution of activities are described, and their representation in the scheduling system MAESTRO is discussed. Initial examples are presented using a sample activity described. Those examples are expanded to include a second activity, and the types of temporal constraints that can obtain between two activities are explored. Soft constraints, or preferences, in activity placement are discussed. Multiple performances of activities are considered, with respect to both hard and soft constraints. The primary methods used in MAESTRO to handle temporal constraints are described as are certain aspects of contingency handling with respect to temporal constraints. A discussion of the overall approach, with indications of future directions for this research, concludes the study.

Britt, Daniel L.↗
NASA NTRS1994

Laboratory simulation of Low Earth Orbit (LEO) atomic oxygen effects

A pulsed fast oxygen atom source has been used extensively over the last 7 years to investigate the effects of ambient oxygen atoms impacting materials placed in low Earth orbit. In this period, we irradiated well over 2000 material samples with 8 km/s oxygen atoms generated in our source. Typical irradiance level is 3 x 10(exp 20) O atoms/sq cm although some materials have been irradiated to fluence levels as high as 6 x 10(exp 21) O atoms/sq cm. The operating principles and characteristics of our source are reviewed along with diagnostic and handling procedures appropriate to material testing. Representative data is presented on the velocity dependence of oxygen atom erosion rates (the PSI source provides oxygen atoms tunable over the velocity range of 5 to 12 km/s) as well as the dependence on material temperature. Specific examples of non-linear oxidative effects related to surface contamination and test duration are also be provided.

Caledonia, George E.↗
NASA NTRS2000

Innovative Liner Concepts: Experiments and Impedance Modeling of Liners Including the Effect of Bias Flow

The study of normal impedance of perforated plate acoustic liners including the effect of bias flow was studied. Two impedance models were developed by modeling the internal flows of perforate orifices as infinite tubes with the inclusion of end corrections to handle finite length effects. These models assumed incompressible and compressible flows, respectively, between the far field and the perforate orifice. The incompressible model was used to predict impedance results for perforated plates with percent open areas ranging from 5% to 15%. The predicted resistance results showed better agreement with experiments for the higher percent open area samples. The agreement also tended to deteriorate as bias flow was increased. For perforated plates with percent open areas ranging from 1% to 5%, the compressible model was used to predict impedance results. The model predictions were closer to the experimental resistance results for the 2% to 3% open area samples. The predictions tended to deteriorate as bias flow was increased. The reactance results were well predicted by the models for the higher percent open area, but deteriorated as the percent open area was lowered (5%) and bias flow was increased. A fit was done on the incompressible model to the experimental database. The fit was performed using an optimization routine that found the optimal set of multiplication coefficients to the non-dimensional groups that minimized the least squares slope error between predictions and experiments. The result of the fit indicated that terms not associated with bias flow required a greater degree of correction than the terms associated with the bias flow. This model improved agreement with experiments by nearly 15% for the low percent open area (5%) samples when compared to the unfitted model. The fitted model and the unfitted model performed equally well for the higher percent open area (10% and 15%).

Kelly, Jeff↗
NASA NTRS1992

Marine Magnetic Data Holdings of World Data Center-a for Marine Geology and Geophysics

The World Data Center-A for Marine Geology and Geophysics is co-located with the Marine Geology & Geophysical Data Center, Boulder, CO. Fifteen million digital marine magnetic trackline measurements are managed within the GEOphysical DAta System (GEODAS). The bulk of these data were collected with proton precision magnetometers under Transit Satellite navigational control. Along-track sampling averages about 1 sample per kilometer, while spatial density, a function of ship's track and survey pattern, range from 4 to 0.02 data points/sq. km. In the near future, the entire geophysical data set will be available on CD-ROM. The Marine Geology and Geophysics Division (World Data Center-A for MGG), of the National Geophysical Data Center, handles a broad spectrum of marine geophysical data, including measurements of bathymetry, magnetics, gravity, seismic reflection subbottom profiles, and side-scan images acquired by ships throughout the world's oceans. Digital data encompass the first three, while the latter two are in analog form, recorded on 35mm microfilm. The marine geophysical digital trackline data are contained in the GEODAS data base which includes 11.6 million nautical miles of cruise trackline coverage contributed by more than 70 organizations worldwide. The inventory includes data from 3206 cruises with 33 million digital records and indexing to 5.3 million track miles of analog data on microfilm.

Sharman, George F.↗
DOE OSTI2024

Coupling Microdroplet-Based Sample Preparation, Multiplexed Isobaric Labeling, and Nanoflow Peptide Fractionation for Deep Proteome Profiling of the Tissue Microenvironment

There is increasing interest in developing in-depth proteomic approaches for mapping tissue heterogeneity in a cell-type-specific manner to better understand and predict the function of complex biological systems such as human organs. Existing spatially resolved proteomics technologies cannot provide deep proteome coverage due to limited sensitivity and poor sample recovery. Herein, we seamlessly combined laser capture microdissection with a low-volume sample processing technology that includes a microfluidic device named microPOTS (microdroplet processing in one pot for trace samples), multiplexed isobaric labeling, and a nanoflow peptide fractionation approach. The integrated workflow allowed us to maximize proteome coverage of laser-isolated tissue samples containing nanogram levels of proteins. We demonstrated that the deep spatial proteomics platform can quantify more than 5000 unique proteins from a small-sized human pancreatic tissue pixel (∼60,000 μm2) and differentiate unique protein abundance patterns in pancreas. Furthermore, the use of the microPOTS chip eliminated the requirement for advanced microfabrication capabilities and specialized nanoliter liquid handling equipment, making it more accessible to proteomic laboratories.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗