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At least 595 records · Page 33

Analysis of edible oil processing options for the BIO-Plex advanced life support system

Edible oil is a critical component of the proposed plant-based Advanced Life Support (ALS) diet. Soybean, peanut, and single-cell oil are the oil source options to date. In terrestrial manufacture, oil is ordinarily extracted with hexane, an organic solvent. However, exposed solvents are not permitted in the spacecraft environment or in enclosed human tests by National Aeronautics and Space Administration due to their potential danger and handling difficulty. As a result, alternative oil-processing methods will need to be utilized. Preparation and recovery options include traditional dehulling, crushing, conditioning, and flaking, extrusion, pressing, water extraction, and supercritical extraction. These processing options were evaluated on criteria appropriate to the Advanced Life Support System and BIO-Plex application including: product quality, product stability, waste production, risk, energy needs, labor requirements, utilization of nonrenewable resources, usefulness of by-products, and versatility and mass of equipment to determine the most appropriate ALS edible oil-processing operation.

NASA Discipline Life Support Systems↗

Satellite-Detected Ocean Ecosystem Response to Volcanic Eruption in the Subarctic Northeast Pacific Ocean

Volcanic eruptions in the Aleutian archipelago during the summer of 2008 deposited large quantities of iron-laden ash to the Subarctic North Pacific Ocean. The surface ocean ecosystem response has been previously characterized using limited in situ and autonomous field measurements and numerical modeling, but basin-scale satellite remote sensing has been limited to a simple description of chlorophyll variability. Here, we show that the ecosystem response is a complex combination of phytoplankton biomass and physiology that can be described with satellite ocean color diagnostics such as the chlorophyll to carbon biomass ratio (Chl:Cphyto) and chlorophyll fluorescence yield. Together, these quantities outline a more complete picture of ecological response spanning unique signals of iron stress (and relief from), photo acclimation, changes in phytoplankton growth rate, increases in biomass, and timescales of decay for these processes.

Ocean Ecosystem↗

Satellite‐Detected Ocean Ecosystem Response to Volcanic Eruptions in the Subarctic Northeast Pacific Ocean

Volcanic eruptions in the Aleutian Archipelago during the summer of 2008 deposited large quantities of iron‐laden ash to the subarctic North Pacific Ocean. The surface ocean ecosystem response has been previously characterized using limited in situ and autonomous field measurements and numerical modeling, but basin‐scale satellite remote sensing has been limited to a simple description of chlorophyll variability. Here, we show that the ecosystem response is a complex combination of phytoplankton biomass and physiology that can be described with satellite ocean color diagnostics such as the chlorophyll‐to‐carbon biomass ratio (Chl:Cphyto) and chlorophyll fluorescence yield. Together, these quantities outline a more complete picture of ecological responses spanning unique signals of iron stress (and relief from), photo acclimation, changes in phytoplankton growth rate, increases in biomass, and timescales of decay for these processes.

T.K. Westberry↗

Detection and Screening of Organic Contaminants in A Riverine System of Georgia Using Non-Targeted Analysis

Numerous organic chemicals exist within aquatic environments, yet effectively screening and prioritizing them is a huge challenge. This study provides a comprehensive investigation into the ecological dynamics of the North Oconee River within Athens-Clarke County, Georgia, with a specific focus on the distribution of 33 identified compounds, including a prominent pesticide. The research, conducted in the riverine ecosystems proximal to the Firefly trail, employs advanced analytical techniques to elucidate potential contamination sources arising from agricultural and urban runoff. Intriguingly, the study reveals North Oconee River near the Firefly Trail as a notable site for heightened pesticide contamination, warranting a meticulous exploration of its origins. Furthermore, the investigation unveils the intricate microbial degradation processes of malathion within the North Oconee River, elucidating the pivotal role played by microbial activity in river water. The detection of degradant byproducts prompts the considerations of bioavailability and toxicity, associating potential implications for the river’s overall ecological health. Ongoing research endeavors to precisely quantify environmental risks and unravel indigenous microbial degradation pathways, presenting pivotal contributions to the scientific community’s understanding of complex riverine ecosystems. This research serves as a foundational piece in informing sustainable environmental management practices and emphasizes the urgency of comprehensive stewardship in safeguarding aquatic ecosystems.

54 ENVIRONMENTAL SCIENCES↗

NASA 1990 Multisensor Airborne Campaigns (MACs) for ecosystem and watershed studies

The Multisensor Airborne Campaign (MAC) focus within NASA's former Land Processes research program was conceived to achieve the following objectives: to acquire relatively complete, multisensor data sets for well-studied field sites, to add a strong remote sensing science component to ecology-, hydrology-, and geology-oriented field projects, to create a research environment that promotes strong interactions among scientists within the program, and to more efficiently utilize and compete for the NASA fleet of remote sensing aircraft. Four new MAC's were conducted in 1990: the Oregon Transect Ecosystem Research (OTTER) project along an east-west transect through central Oregon, the Forest Ecosystem Dynamics (FED) project at the Northern Experimental Forest in Howland, Maine, the MACHYDRO project in the Mahantango Creek watershed in central Pennsylvania, and the Walnut Gulch project near Tombstone, Arizona. The OTTER project is testing a model that estimates the major fluxes of carbon, nitrogen, and water through temperate coniferous forest ecosystems. The focus in the project is on short time-scale (days-year) variations in ecosystem function. The FED project is concerned with modeling vegetation changes of forest ecosystems using remotely sensed observations to extract biophysical properties of forest canopies. The focus in this project is on long time-scale (decades to millenia) changes in ecosystem structure. The MACHYDRO project is studying the role of soil moisture and its regulating effects on hydrologic processes. The focus of the study is to delineate soil moisture differences within a basin and their changes with respect to evapotranspiration, rainfall, and streamflow. The Walnut Gulch project is focused on the effects of soil moisture in the energy and water balance of arid and semiarid ecosystems and their feedbacks to the atmosphere via thermal forcing.

Wickland, Diane E.↗

Cross-Disciplinarity in the Advance of Antarctic Ecosystem Research

The biodiversity, ecosystem services and climate variability of the Antarctic continent, and the Southern Ocean are major components of the whole Earth system. Antarctic ecosystems are driven more strongly by the physical environment than many other marine and terrestrial ecosystems. As a consequence, to understand ecological functioning, cross-disciplinary studies are especially important in Antarctic research. The conceptual study presented here is based on a workshop initiated by the Research Programme Antarctic Thresholds - Ecosystem Resilience and Adaption of the Scientific Committee on Antarctic Research, which focused on challenges in identifying and applying cross-disciplinary approaches in the Antarctic. Novel ideas, and first steps in their implementation, were clustered into eight themes, ranging from scale problems, risk maps, organism and ecosystem responses to multiple environmental changes, to evolutionary processes. Scaling models and data across different spatial and temporal scales were identified as an overarching challenge. Approaches to bridge gaps in the research programmes included multi-disciplinary monitoring, linking biomolecular findings and simulated physical environments, as well as integrative ecological modelling. New strategies in academic education are proposed. The results of advanced cross-disciplinary approaches can contribute significantly to our knowledge of ecosystem functioning, the consequences of climate change, and to global assessments that ultimately benefit humankind.

Scaling↗

Synthetic soils for ecological and synthetic biology applications

Soils are heterogeneous and dynamic systems characterized by complex physical, chemical, and biological interactions. Understanding these interactions is critical, as they influence plant productivity, global biogeochemical cycles, and ecosystem resilience. While ecologists have long studied soils in field, greenhouse, and laboratory settings, their complexity and heterogeneity make it challenging to pinpoint key properties driving biological processes and derive mechanistic insights. Advancements in synthetic biology, which seeks to engineer and control biological processes in soils, have increased the demand for standardized and controllable experimental platforms. These platforms, referred to here as ‘synthetic soils’, are systems designed to reproduce selected physicochemical characteristics of natural soils in a simplified and defined format, allowing scientists to systematically change soil physicochemical properties (i.e. texture, mineralogy, pH) to study how biological components (i.e. microbes, plants, soil fauna, etc.) respond to, modify, or interact within these controlled environments. This review explores existing synthetic soils, their advantages, limitations, and applications in ecology and synthetic biology, and discusses potential directions for their future development.

artificial soils↗

Activity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane

Microbial metabolisms and interactions that facilitate subsurface conversions of recalcitrant carbon to methane are poorly understood. We deployed an in situ enrichment device in a subsurface coal seam in the Powder River Basin (PRB), USA, and used BONCAT-FACS-Metagenomics to identify translationally active populations involved in methane generation from a variety of coal-derived aromatic hydrocarbons. From the active fraction, high-quality metagenome-assembled genomes (MAGs) were recovered for the acetoclastic methanogen, Methanothrix paradoxum, and a novel member of the Chlorobi with the potential to generate acetate via the Pta-Ack pathway. Members of the Bacteroides and Geobacter also encoded Pta-Ack and together, all four populations had the putative ability to degrade ethylbenzene, phenylphosphate, phenylethanol, toluene, xylene, and phenol. Metabolic reconstructions, gene analyses, and environmental parameters also indicated that redox fluctuations likely promote facultative energy metabolisms in the coal seam. The active "Chlorobi PRB" MAG encoded enzymes for fermentation, nitrate reduction, and multiple oxygenases with varying binding affinities for oxygen. "M. paradoxum PRB" encoded an extradiol dioxygenase for aerobic phenylacetate degradation, which was also present in previously published Methanothrix genomes. Finaly, these observations outline underlying processes for bio-methane from subbituminous coal by translationally active populations and demonstrate activity-based metagenomics as a powerful strategy in next generation physiology to understand ecologically relevant microbial populations.

59 BASIC BIOLOGICAL SCIENCES↗

Human–Earth system interactions under climate change

This special issue, titled ‘Focus on human–Earth system interactions under climate change,’ compiles cutting-edge research that deepens our understanding of the complex feedback mechanisms between anthropogenic activities and Earth system processes in the context of a changing climate (figure 1). The featured articles employ a diverse array of methodologies—including field studies, remote sensing, modeling, and socio-ecological analyses—to explore these interconnections. A unifying theme across these contributions is the imperative to bridge disciplinary divides, integrating physical Earth sciences with social sciences to fully capture the spectrum of human-Earth system interactions. Key topics addressed the impacts of human activities on natural systems, encompassing land-use change and water resource management on biogeochemistry, climate dynamics, and feedback on the climate system and socioeconomics from local to global scales under a changing climate.

Li, Huidong [Vanderbilt Univ., Nashville, TN (Unit↗

Mineral separation and recycle in a Controlled Ecological Life Support System (CELSS)

The background of the mineral nutrition needs of plants are examined along with the applicability of mineral control and separation to a controlled ecological life support system (CELSS). Steps that may be taken in a program to analytically define and experimentally test key mineral control concepts in the nutritional and waste processing loops of a CELSS are delineated.

Ballou, E. V.↗

Autonomous support for microorganism research in space

A preliminary design for performing on orbit, autonomous research on microorganisms and cultured cells/tissues is presented. An understanding of gravity and its effects on cells is crucial for space exploration as well as for terrestrial applications. The payload is designed to be compatible with the Commercial Experiment Transporter (COMET) launch vehicle, an orbiter middeck locker interface, and with Space Station Freedom. Uplink/downlink capabilities and sample return through controlled reentry are available for all carriers. Autonomous testing activities are preprogrammed with in-flight reprogrammability. Sensors for monitoring temperature, pH, light, gravity levels, vibrations, and radiation are provided for environmental regulation and experimental data collection. Additional experimental data acquisition includes optical density measurement, microscopy, video, and film photography. On-board full data storage capabilities are provided. A fluid transfer mechanism is utilized for inoculation, sampling, and nutrient replenishment of experiment cultures. In addition to payload design, representative experiments were developed to ensure scientific objectives remained compatible with hardware capabilities. The project is defined to provide biological data pertinent to extended duration crewed space flight including crew health issues and development of a Controlled Ecological Life Support System (CELSS). In addition, opportunities are opened for investigations leading to commercial applications of space, such as pharmaceutical development, modeling of terrestrial diseases, and material processing.

Fleet, Mary L.↗

Autonomous support for microorganism research in space

A preliminary design for performing on-orbit, autonomous research on microorganisms and cultured cells/tissues is presented. An understanding of gravity and its effects on cells is crucial for space exploration as well as for terrestrial applications. The payload is designed to be compatible with the COMmercial Experiment Transported (COMET) launch vehicle, an orbiter middeck locker interface, and with Space Station Freedom. Uplink/downlink capabilities and sample return through controlled reentry are available for all carriers. Autonomous testing activities are preprogrammed with inflight reprogrammability. Sensors for monitoring temperature, pH, light, gravity levels, vibration, and radiation are provided for environmental regulation and experimental data collection. Additional experiment data acquisition includes optical density measurement, microscopy, video, and file photography. Onboard full data storage capabilities are provided. A fluid transfer mechanism is utilized for inoculation, sampling, and nutrient replenishment of experiment cultures. In addition to payload design, representative experiments were developed to ensure scientific objectives remained compatible with hardware capabilities. The project is defined to provide biological data pertinent to extended duration crewed space flight including crew health issues and development of a Controlled Ecological Life Support System (CELSS). In addition, opportunities are opened for investigations leading to commercial applications of space, such as pharmaceutical development, modeling of terrestrial diseases, and material processing.

Luttges, M. W.↗

Configurable technology development for reusable control and monitor ground systems

The control monitor unit (CMU) uses configurable software technology for real-time mission command and control, telemetry processing, simulation, data acquisition, data archiving, and ground operations automation. The base technology is currently planned for the following control and monitor systems: portable Space Station checkout systems; ecological life support systems; Space Station logistics carrier system; and the ground system of the Delta Clipper (SX-2) in the Single-Stage Rocket Technology program. The CMU makes extensive use of commercial technology to increase capability and reduce development and life-cycle costs. The concepts and technology are being developed by McDonnell Douglas Space and Defense Systems for the Real-Time Systems Laboratory at NASA's Kennedy Space Center under the Payload Ground Operations Contract. A second function of the Real-Time Systems Laboratory is development and utilization of advanced software development practices.

Uhrlaub, David R.↗

Data and scripts associated with “Non-random processes impacting organic matter chemistry are maximized in mid-order streams”

NOTE: The manuscript associated with this data package is currently in review. The data may be revised based on reviewer feedback. Upon manuscript acceptance, this data package will be updated with the final dataset and additional metadata. This data package is associated with the publication “Non-random processes impacting organic matter chemistry are maximized in mid-order streams” submitted to Limnology and Oceanography (L&O) by Danczak et al. (in review). This package contains data and scripts used to investigate dissolved organic matter (DOM) molecular chemistry and diversification processes across 47 surface-water sampling sites in the Yakima River Basin, Washington, USA, during an August 2021 sampling campaign. The package contains analyses of ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS), geochemical measurements, geospatial attributes, molecular diversity, and meta-metabolome ecological null models needed to reproduce the main manuscript results. The underlying field data were pulled from exising data packages at https://doi.org/10.15485/1892052 (Fulton et al., 2022) and https://doi.org/10.15485/1898914 (Grieger et al., 2022). For details on how to navigate data packages generated by this project, see https://data.ess-dive.lbl.gov/portals/PNNLRiverCorridorSFA/About. We thank the following organizations for providing access to field locations for sample collection: the United States Forest Service, Washington Department of Fish and Wildlife, Washington Department of Natural Resources, the Confederated Tribes and Bands of the Yakama Nation, and the Cowiche Canyon Conservatory. Research was conducted under Washington State Parks and Recreation Commission Scientific Research Permit #210901. We are grateful to the Yakama Nation Tribal Council and Yakama Nation Fisheries for their collaboration in facilitating sample collection and ensuring data usage aligns with their values and worldview. This data package contains an R-Markdown file for analyses and five folders: (1) Data, (2) Geospatial Data, (3) Supplemental_Files, (5) Figures_pdf, (4) and src. The Data folder contains tabular inputs and derived files used in the manuscript analysis. The Geospatial Data folder contains climate and water-balance, hydrologic, land-cover, population/regional water-use, stream, topographic, and stream-order attribute CSV files. The src folder contains scripts used to process data, run analyses, and generate figures. The Figures_pdf folder contains manuscript figure outputs. The Supplemental_Files folder contains supplemental analysis products. All files are .csv, .pdf, .html, .png, .R, .Rmd, .svg, or .tre. This data package is associated with the rcfsa-RC2-SPS_Null_Modeling repository found at https://github.com/river-corridors-sfa/rcfsa-RC2-SPS_Null_Modeling.

54 ENVIRONMENTAL SCIENCES↗

A Probabilistic Method of Assessing Carbon Accumulation Rate at Imnavait Creek Peatland, Arctic Long Term Ecological Research Station, Alaska

Arctic peatlands are an important part of the global carbon cycle, accumulating atmospheric carbon as organic matter since the Late glacial. Current methods for understanding the changing efficiency of the peatland carbon sink rely on peatlands with an undisturbed stratigraphy. Here we present a method of estimating primary carbon accumulation rate from a site where permafrost processes have either vertically or horizontally translocated nearby carbon-rich sediment out of stratigraphic order. Briefly, our new algorithm estimates the probability of the age of deposition of a random increment of sediment in the core. The method assumes that if sediment age is measured at even depth increments, dates are more likely to occur during intervals of higher accumulation rate and vice versa. Multiplying estimated sedimentation rate by measured carbon density yields carbon accumulation rate. We perform this analysis at the Imnavait Creek Peatland, near the Arctic Long Term Ecological Research network site at Toolik Lake, Alaska. Using classical radiocarbon age modeling, we find unreasonably high rates of carbon accumulation at various Holocene intervals. With our new method, we find accumulation rate changes that are in improved agreement within the context of other sites throughout Alaska and the rest of the Circum-Arctic region.

carbon accumulation;Imnavait;peatlands;permafrost;↗

Informing climate adaptation strategies using ecological simulation models and spatial decision support tools

Introduction: Forest landscapes offer resources and ecosystem services that are vital to the social, economic, and cultural well-being of human communities, but managing for these provisions can require socially and ecologically relevant trade-offs. We designed a spatial decision support model to reveal trade-offs and synergies between ecosystem services in a large eastern Cascade Mountain landscape in Washington State, USA. Methods: We used process-based forest landscape (LANDIS-II) and hydrology (DHSVM) models to compare outcomes associated with 100 years of simulated forest and wildfire dynamics for two management scenarios, Wildfire only and Wildfire + Treatments. We then examined the strength and spatial distribution of potential treatment effects and trends in a set of resources and ecosystem services over the simulation period. Results: We found that wildfire area burned increased over time, but some impacts could be mitigated by adaptation treatments. Treatment benefits were not limited to treated areas. Interestingly, we observed neighborhood benefits where fire spread and severity were reduced not only in treated patches but in adjacent patches and landscapes as well, creating potential synergies among some resource benefits and services. Ordinations provided further evidence for two main kinds of outcomes. Positive ecological effects of treatments were greatest in upper elevation moist and cold forests, while positive benefits to human communities were aligned with drier, low- and mid-elevation forests closer to the wildland urban interface. Discussion: Our results contribute to improved understanding of synergies and tradeoffs linked to adaptation and restoration efforts in fire-prone forests and can be used to inform management aimed at rebuilding resilient, climate-adapted landscapes.

54 ENVIRONMENTAL SCIENCES↗

NASA to Survey Earth's Resources

A wide variety of the natural resources of earth and man's management of them will be studied by an initial group of foreign and domestic scientists tentatively chosen by the National Aeronautics and Space Administration to analyze data to be gathered by two earth-orbiting spacecraft. The spacecraft are the first Earth Resources Technology Satellite (ERTS-A) and the manned Skylab which will carry an Earth Resources Experiment Package (EREP). In the United States, the initial experiments will study the feasibility of remote sensing from a satellite in gathering information on ecological problems. The objective of both ERTS and EREP aboard Skylab is to obtain multispectral images of the surface of the earth with high resolution remote sensors and to process and distribute the images to scientific users in a wide variety of disciplines. The ERTS-A, EREP, and Skylab systems are described and their operation is discussed.

Mittauer, R. T.↗