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At least 613 records · Page 34
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NASA NTRS2000

Numerical Simulations of TRMM LBA, TOGA, COARE, GATE, ARM and PRESTORM Convective Systems: Sensitivity tests on Microphysical Processes

The 3D Goddard Cumulus Ensemble (GCE) model was utilized to examine the behavior and response of simulated deep tropical cloud systems that occurred over the west Pacific warm pool region, the Atlantic ocean and the central United States. The periods chosen for simulation were convectively active periods during TOGA-COARE (February 22 1993, December 11-17, 1992; December 19-28, February 9-13, 1993), GATE (September 4, 1974), LBA (January 26 and February 23, 1998), ARM (1997 IOP) and PRESTORM (June 11, 1985). We will examine differences in the microphysics for both warm rain and ice processes (evaporation /sublimation and condensation/ deposition), Q1 (Temperature), Q2 (Water vapor) and Q3 (momentum both U and V) budgets for these three convective events from different large-scale environments. The contribution of stratiform precipitation and its relationship to the vertical shear of the large-scale horizontal wind will also be examined. New improvements to the GCE model (i.e., microphysics: 4ICE two moments and 3ICE one moment; advection schemes) as well as their sensitivity to the model results will be discussed. Preliminary results indicated that various microphysical schemes could have a major impact on stratiform formation as well as the size of convective systems. However, they do not change the major characteristics of the convective systems, such as: arc shape, strong rotational circulation on both ends of system, heavy precipitation along the leading edge of systems.

Tao, W.-K.↗
NASA NTRS2000

Confirmation of NLDN Long Range Strike Locations with LIS Observations

This study compares the lightning locations reported by the National Lightning Detection Network (NLDN) with the lightning locations determined by the Lightning Imaging Sensor (LIS). The NLDN system identifies the rf signature of cloud-to-ground lightning. The LIS data is the top level of a hierarchy of optical data objects. The centroid and timing of each LIS lightning activity center are compared with each flash in a subset of the NLDN long range lightning location data in a portion of the Atlantic Ocean and the Caribbean Sea consisting of those locations more than 625 km from any sensor. This subset is produced by analyzing each reported NLDN location to determine if that location is within the LIS field of view at the time of the reported flash. The Tropical Rainfall Measuring Mission Satellite (TRMM) orbit limits the cross-sensor comparison to tropical and sub-tropical regions. Because the rf-detection system depends on ionospheric propagation conditions, a separate analysis was made for daylight conditions at both source and sensor as well as nighttime at both places. A full year of data is compared to provide an adequate sample of each data set. Confirmation of lightning in the general location of the NLDN report is established when LIS detected one or more centers of lightning activity within a 2 degree radius from the NLDN location.

Boeck, William↗
NASA NTRS2001

The Goddard Cumulus Ensemble Model: Model Description and Its Application for Studying the TOGA COARE and GATE Convective Systems

The Goddard Cumulus Ensemble (GCE) model was utilized in two and three dimensions in order to examine the behavior and response of simulated deep tropical cloud systems occurred in west Pacific warm pool region and Atlantic ocean. The periods chosen for simulation were convectively active period over the TOGA-COARE IFA (19-27 December 1992) and GATE (September 1 to 7, 1974). The TOGA COARE IFA period was also in the framework of the GEWEX Cloud System Study (GCSS) WG4 case 2. We will examine the differences between the microphysics (warm rain and ice processes, evaporation/sublimation and condensation/deposition), Q1 (Temperature) and Q2 (Water vapor) budgets between these two convective events occurred in different large-scale environments. The contribution of stratiform precipitation and its relationship to the vertical shear of the large-scale horizontal wind will also be examined. The results from GCSS model intercomparsion will be presented. The new improvements (i.e., microphysics, cloud radiation interaction, surface processes and numerical advection scheme) of the GCE model as well as their sensitivity to the model results will be discussed.

Tao, Wei-Kuo↗
NASA NTRS2000

Remotely Sensed Precipitation from the January 2000 East Coast Blizzard

Initial precipitation forecasts of the January 2000 blizzard were unable to adequately alert emergency response along the eastern seaboard. Cyclogenesis, over the Gulf of Mexico was a contributing cause of that problem. We present distributions of precipitation derived from spaceborne microwave measurements from the Special Sensor Microwave/ Imager (SSM/I) and the TRMM Microwave Imager (TMI) supplemented by NWS operational radar and the National lightning Detection Network (NLDN) Long-Range (LR) product. The combined NWS radar and NLDN-LR data revealed the location of precipitation between 1/23 and 1/27/2000 over the US, the Gulf and the Atlantic Ocean. The remotely sensed precipitation distributions are compared to the precipitation inferred from coincident TMI observations.

Chang, D.-E.↗
NASA NTRS2001

Global Distribution and Sources of Volatile and Nonvolatile Aerosol In the Remote Troposphere

Airborne measurements of aerosol (Condensation Nuclei, CN) and selected trace gases made in the areas of the North Atlantic Ocean during SONEX (October/November 1997), and the south tropical Pacific Ocean during PEM-Tropics A (September/October 1996) and PEM-Tropics B (March/April 1999) have been analyzed. Emphasis is on the interpretations of variations in the number densities of the fine (>17 nm) and ultrafine (>8 nm) CN in the upper troposphere (8-12 km). These data suggest that large number densities of highly volatile CN (10(exp 4)-10(exp 5)/cu cm) are present in the clean upper troposphere with highest values over the tropical1subtropical region. Through marine convection and long-distance horizontal transport, volatile CN originating from the tropical/subtropical regions can frequently impact the abundance of aerosol in the middle and upper troposphere at mid to high latitudes. Nonvolatile aerosol particles behave in a manner similar to tracers of combustion (CO) and photochemical pollution (PAN), implying a source from continental pollution of industrial or biomass burning origin. In the upper troposphere, we find that volatile and nonvolatile partials number densities are inversely correlated. An aerosol microphysical model is used to suggest that coagulation of fine volatile particles with fewer larger nonvolatile particles provides one possible mechanism for this relationship. It appears that nonvolatile particles, of principally anthropogenic origin,provide a highly efficient removal process for the fine volatile aerosol.

Singh, Hanwant B.↗
NASA NTRS2001

Water Vapor Tracers as Diagnostics of the Regional Hydrologic Cycle

Numerous studies suggest that local feedback of evaporation on precipitation, or recycling, is a significant source of water for precipitation. Quantitative results on the exact amount of recycling have been difficult to obtain in view of the inherent limitations of diagnostic recycling calculations. The current study describes a calculation of the amount of local and remote sources of water for precipitation, based on the implementation of passive constituent tracers of water vapor (termed water vapor tracers, WVT) in a general circulation model. In this case, the major limitation on the accuracy of the recycling estimates is the veracity of the numerically simulated hydrological cycle, though we note that this approach can also be implemented within the context of a data assimilation system. In this approach, each WVT is associated with an evaporative source region, and tracks the water until it precipitates from the atmosphere. By assuming that the regional water is well mixed with water from other sources, the physical processes that act on the WVT are determined in proportion to those that act on the model's prognostic water vapor. In this way, the local and remote sources of water for precipitation can be computed within the model simulation, and can be validated against the model's prognostic water vapor. Furthermore, estimates of precipitation recycling can be compared with bulk diagnostic approaches. As a demonstration of the method, the regional hydrologic cycles for North America and India are evaluated for six summers (June, July and August) of model simulation. More than 50% of the precipitation in the Midwestern United States came from continental regional tracers, and the local source was the largest of the regional tracers (14%). The Gulf of Mexico and Atlantic 2 regions contributed 18% of the water for Midwestern precipitation, but further analysis suggests that the greater region of the Tropical Atlantic Ocean may also contribute significantly. In general, most North American land regions showed a positive correlation between evaporation and recycling ratio (except the Southeast United States) and negative correlations of recycling ratio with precipitation and moisture transport (except the Southwestern United States). The Midwestern local source is positively correlated with local evaporation, but it is not correlated with water vapor transport. This is contrary to bulk diagnostic estimates of precipitation recycling. In India, the local source of precipitation is a small percentage of the precipitation owing to the dominance of the atmospheric transport of oceanic water. The southern Indian Ocean provides a key source of water for both the Indian continent and the Sahelian region.

Bosilovich, Michael G.↗
NASA NTRS2001

Evaluation of Bogus Vortex Techniques Using Four-Dimensional Variational Data Assimilation

The effectiveness of a four-dimensional variational data assimilation (4-DVAR) technique for creating "bogus" vortices in numerical simulations of hurricanes is evaluated in this study. A series of numerical experiments is conducted to generate initial vortices for Hurricane Georges and Bonnie (1998) in the Atlantic Ocean by assimilating bogus sea-level pressure and wind information into a mesoscale numerical model (MM5). Several different strategies are tested for investigating the sensitivity of the initial vortex representation to the type of bogus information.

Pu, Zhao-Xia↗
NASA NTRS2001

Water Vapor Tracers as Diagnostics of the Regional Hydrologic Cycle

Numerous studies suggest that local feedback of surface evaporation on precipitation, or recycling, is a significant source of water for precipitation. Quantitative results on the exact amount of recycling have been difficult to obtain in view of the inherent limitations of diagnostic recycling calculations. The current study describes a calculation of the amount of local and remote geographic sources of surface evaporation for precipitation, based on the implementation of three-dimensional constituent tracers of regional water vapor sources (termed water vapor tracers, WVT) in a general circulation model. The major limitation on the accuracy of the recycling estimates is the veracity of the numerically simulated hydrological cycle, though we note that this approach can also be implemented within the context of a data assimilation system. In the WVT approach, each tracer is associated with an evaporative source region for a prognostic three-dimensional variable that represents a partial amount of the total atmospheric water vapor. The physical processes that act on a WVT are determined in proportion to those that act on the model's prognostic water vapor. In this way, the local and remote sources of water for precipitation can be predicted within the model simulation, and can be validated against the model's prognostic water vapor. As a demonstration of the method, the regional hydrologic cycles for North America and India are evaluated for six summers (June, July and August) of model simulation. More than 50% of the precipitation in the Midwestern United States came from continental regional sources, and the local source was the largest of the regional tracers (14%). The Gulf of Mexico and Atlantic regions contributed 18% of the water for Midwestern precipitation, but further analysis suggests that the greater region of the Tropical Atlantic Ocean may also contribute significantly. In most North American continental regions, the local source of precipitation is correlated with total precipitation. There is a general positive correlation between local evaporation and local precipitation, but it can be weaker because large evaporation can occur when precipitation is inhibited. In India, the local source of precipitation is a small percentage of the precipitation owing to the dominance of the atmospheric transport of oceanic water. The southern Indian Ocean provides a key source of water for both the Indian continent and the Sahelian region.

Bosilovich, Michael G.↗
NASA NTRS2001

MODIS Observation of Aerosols over Southern Africa During SAFARI 2000: Data, Validation, and Estimation of Aerosol Radiative Forcing

Aerosol properties, including optical thickness and size parameters, are retrieved operationally from the MODIS sensor onboard the Terra satellite launched on 18 December 1999. The predominant aerosol type over the Southern African region is smoke, which is generated from biomass burning on land and transported over the southern Atlantic Ocean. The SAFARI-2000 period experienced smoke aerosol emissions from the regular biomass burning activities as well as from the prescribed burns administered on the auspices of the experiment. The MODIS Aerosol Science Team (MAST) formulates and implements strategies for the retrieval of aerosol products from MODIS, as well as for validating and analyzing them in order to estimate aerosol effects in the radiative forcing of climate as accurately as possible. These activities are carried out not only from a global perspective, but also with a focus on specific regions identified as having interesting characteristics, such as the biomass burning phenomenon in southern Africa and the associated smoke aerosol, particulate, and trace gas emissions. Indeed, the SAFARI-2000 aerosol measurements from the ground and from aircraft, along with MODIS, provide excellent data sources for a more intensive validation and a closer study of the aerosol characteristics over Southern Africa. The SAFARI-2000 ground-based measurements of aerosol optical thickness (AOT) from both the automatic Aerosol Robotic Network (AERONET) and handheld Sun photometers have been used to validate MODIS retrievals, based on a sophisticated spatio-temporal technique. The average global monthly distribution of aerosol from MODIS has been combined with other data to calculate the southern African aerosol daily averaged (24 hr) radiative forcing over the ocean for September 2000. It is estimated that on the average, for cloud free conditions over an area of 9 million square kin, this predominantly smoke aerosol exerts a forcing of -30 W/square m C lose to the terrestrial surface and -10 W/square m at the top of the atmosphere (TOA). While cooling the surface and Earth system, the difference of 20 W/square m is energy that heats the atmosphere.

Ichoku, Charles↗
NASA NTRS2002

STS-108 Mission Highlights Resource Tape

This is Part 1 of a three part video series which provides highlights of the STS-108 mission during which the Expedition Three crew (Commander Frank Culbertson) of the International Space Station (ISS) was relieved by the Expedition Four crew (Commander Yury Onfrienko). For the other parts of the series, refer to 'STS-108 Mission Highlights Resource Tape, Part 2 of 3' (Internal ID 2002049330) and 'STS-108 Mission Highlights Resource Tape, Part 3 of 3' (Internal ID 2002049329). During this video, the STS-108 crew of the Space Shuttle Endeavour (Commander Dom Gorie), as well as the Expedition Four crew, are shown preparing for liftoff at Kennedy Space Center (KSC). Preparations include preflight suiting up, and the strapping in of the crew by support staff. The launch is shown from the Launch Control Center. Following the orbital entry of Endeavour, there is an inspection of the shuttle's payload bay. There is some flight deck activity as the crew prepares for the rendezvous and docking of Endeavour with the ISS. Following the docking, the Expedition Three crew welcomes the others aboard the station, and both crews are briefed. There is an unobstructed view of the South Atlantic Ocean and the coast line of Argentina which includes Peninsula Valdes and Golfo Nuevo. The Raffaello Multipurpose Logistics Module (MPLM) is unberthed from the shuttle payload bay and attached to the ISS.

Source record↗
NASA NTRS1997

Direct Aerosol Radiative Forcing: Calculations and Measurements from the Tropospheric

Radiative forcing is defined as the change in the net (downwelling minus upwelling) radiative flux at a given level in the atmosphere. This net flux is the radiative power density available to drive climatic processes in the earth-atmosphere system below that level. Recent research shows that radiative forcing by aerosol particles is a major source of uncertainty in climate predictions. To reduce those uncertainties, TARFOX was designed to determine direct (cloud-free) radiative forcing by the aerosols in one of the world's major industrial pollution plumes--that flowing from the east coast of the US over the Atlantic Ocean. TARFOX measured a variety of aerosol radiative effects (including direct forcing) while simultaneously measuring the chemical, physical, and optical properties of the aerosol particles causing those effects. The resulting data sets permit a wide variety of tests of the consistency, or closure, among the measurements and the models that link them. Because climate predictions use the same or similar model components, closure tests help to assess and reduce prediction uncertainties. In this work we use the TARFOX-determined aerosol, gas, and surface properties to compute radiative forcing for a variety of aerosol episodes, with inadvisable optical depths ranging from 0.07 to 0.6. We calculate forcing by several techniques with varying degrees of sophistication, in part to test the range of applicability of simplified techniques--which are often the only ones feasible in climate predictions by general circulation models (GCMs). We then compare computed forcing to that determined from: (1) Upwelling and downwelling fluxes (0.3-0.7 mm and 0.7-3.0 mm) measured by radiometers on the UK MRF C-130. and (2) Daily average cloud-free absorbed solar and emitted thermal radiative flux at the top of the atmosphere derived from the AVHRR radiometer on the NOAA- 14 satellite. The calculations and measurements all yield aerosol direct radiative forcing in the range -50 to -190 W sq m per unit inadvisable optical depth. The magnitudes are about 15 to 100 times larger than the global-average direct forcing expected for the global-average sulfate aerosol optical depth of 0.04. The reasons for the larger forcing in TARFOX include the relatively large optical depths and the focus on cloud-free, daytime conditions over the dark ocean surface. These are the conditions that produce the actual major radiative forcing events that contribute to any global-average climate effect. Detailed comparisons of calculated and measured forcings for specific events are used for more refined tests of closure.

Russell, P. B.↗
NASA NTRS1997

An Overview of the Tropospheric Aerosol Radiative Forcing Observational Experiment

Aerosol effects on atmospheric radiation are a leading source of uncertainty in predicting future climate. As a result, the International Global Atmospheric Chemistry Program has established a Focus on Atmospheric Aerosols (IGAC/FAA) and endorsed a series of aerosol field campaigns. TARFOX, the second in the IGAC/FAA series, was designed to reduce this uncertainty by measuring aerosol properties and effects in the US eastern seaboard, where one of the world's major plumes of industrial haze moves from the continent over the Atlantic Ocean. TARFOX's objectives are to: 1. Make simultaneous measurements of: (a) aerosol effects on radiation fields, and (b) the chemical, physical, and optical properties of the aerosols causing those effects. 2. Perform a variety of closure studies by using overdetermined data sets to test the mutual consistency of measurements and calculations of a wide range of aerosol properties and effects. 3. Use the results of the closure studies to assess and reduce uncertainties in estimates of aerosol radiative forcing, as well as to guide future field programs. An important subset of the closure studies is tests and improvements of algorithms used to derive aerosol properties and radiative effects from satellite measurements. The TARFOX Intensive Field Period (IFP) was conducted July 10-31, 1996. It included coordinated measurements from four satellites (GOES-8, NOAA-14, ERS-2, LANDSAT), four aircraft (ER-2, C-130, C-131, and a modified Cessna), land sites, and ships. A variety of aerosol conditions was sampled, ranging from relatively clean behind frontal passages to moderately polluted with aerosol optical depths exceeding 0.5 at mid-visible wavelengths. The latter conditions included separate incidents of enhancements caused primarily by anthropogenic sources and another incident of enhancement apparently influenced by recent fog processing. Spatial gradients of aerosol optical thickness were sampled to aid in isolating aerosol effects from other radiative effects and to more tightly constrain closure tests, including those of satellite retrievals. This talk gives an overview of TARFOX goals, rationale, methods, and initial key findings.

Russell, P. B.↗
NASA NTRS1998

Airspace Complexity and its Application in Air Traffic Management

The United States Air Traffic Management (ATM) system provides services to enable safe, orderly and efficient aircraft operations within the airspace over the continental United States and over large portions of the Pacific and Atlantic Oceans, and the Gulf of Mexico. It consists of two components, Air Traffic Control (ATC) and Traffic Flow Management (TFM). The ATC function ensures that the aircraft within the airspace are separated at all times while the TFM function organizes the aircraft into a flow pattern to ensure their safe and efficient movement. In order to accomplish the ATC and TFM functions, the airspace over United States is organized into 22 Air Route Traffic Control Centers (ARTCCs). The Center airspace is stratified into low-altitude, high-altitude and super-high altitude groups of Sectors. Each vertical layer is further partitioned into several horizontal Sectors. A typical ARTCC airspace is partitioned into 20 to 80 Sectors. These Sectors are the basic control units within the ATM system.

Sridhar, Banavar↗
NASA NTRS2002

Thermococcus sulfurophilus sp. nov., a New Hyperthermophilic, Sulfur-Reducing Archaeon Isolated from Deep-Sea Hydrothermal Vent

A new hyperthermophilic, anaerobic, sulfur-reducing, organo-heterotrophic archaeon, strain OGL-20P, was isolated from "black smoker" chimney material at the Rainbow hydrothermal vent site in the Atlantic Ocean (36.2 N; 33.9 W). The cells of strain OGL-20P have irregular coccoid shape and are motile with a single flagellum. Growth occurs within pH range of 5.5-8.2 (optimal at pH 7.0-7.2), salinity range of 1-5% NaCl (optimal concentration 3% NaCl wt/vol), and temperature range of +55 C to +94 C (optimal growth at +83 C to +85 C). Strain OGL-20P is resistant to freezing (at -20 C). New isolate is strictly anaerobic with sulfur-type of respiration. A limited number of compounds are utilized as electron donors, including peptone, becto-tryptone, casamino-acids, and yeast extract but does not grow with separate amino acids. Sulfur and Iron can be used as electron acceptors; but not sulfate, sulfite, thiosulfate or nitrate. Strain OGL-20P is resistant to chloramphenicol, kanamycin, and gentamycin. Growth of str. OGL20P is inhibited by tetracyclin but not by Na2MoO4. The G+C content of DNA is 57.2 mol%. The 16S ribosomal RNA sequence analysis allows one to classify strain OGL-20P as a representative of a now species of Thermococcus genus. The name Thermococcus sulfurophilus op. nov., was suggested for the new isolate, type strain OGL-20P (sup T) (= ATCC BAA_394 (sup T) = DSM...(supT)).

Pikuta, Elena V.↗
NASA NTRS2002

STS-111 Flight Day 10 Highlights Replay

The most important highlight of flight day 10 of STS-111 was the undocking of the Leonardo Multi Purpose Logistical Module (MPLM) from the Unity module of the International Space Station (ISS). The undocking is shown, as is Leonardo resting in Endeavour's payload bay. The video also includes an interview, and footage of the West Africa and the Atlantic Ocean, Gibraltar and the Mediterranean Sea, Italy, Mexico and the Pacific Ocean, Hispanola, an unknown land mass, and unknown mountains.

Source record↗
NASA NTRS2002

Characterization of Dust Properties during ACE-Asia and PRIDE: A Column Satellite-Surface Perspective

Many recent field experiments are designed to study the compelling variability in spatial and temporal scale of both pollution-derived and naturally occurring aerosols, which often exist in high concentration over particular pathways around the globe. For example, the ACE-Asia (Aerosol Characterization Experiment-Asia) was conducted from March-May 2001 in the vicinity of the Taklimakan and Gobi deserts, East Coast of China, Yellow Sea, Korea, and Japan, along the pathway of Kosa (severe events that blanket East Asia with yellow desert dust, peaked in the Spring season). The PRIDE (Puerto RIco Dust Experiment, July 2000) was designed to measure the properties of Saharan dust transported across the Atlantic Ocean to the Caribbean. Dust particles typically originate in desert areas far from polluted urban regions. During transport, dust layers can interact with anthropogenic sulfate and soot aerosols from heavily polluted urban areas. Added to the complex effects of clouds and natural marine aerosols, dust particles reaching the marine environment can have drastically different properties than those from the source. Thus, understanding the unique temporal and spatial variations of dust aerosols is of special importance in regional-to-global climate issues such as radiative forcing, the hydrological cycle, and primary biological productivity in the ocean. During ACE-Asia and PRIDE we had measured aerosol physical/optical/radiative properties, column precipitable water amount, and surface reflectivity over homogeneous areas from ground-based remote sensing. The inclusion of flux measurements permits the determination of aerosol radiative flux in addition to measurements of loading and optical depth. At the time of the Terra/MODIS, SeaWiFS, TOMS and other satellite overpasses, these ground-based observations can provide valuable data to compare with satellite retrievals over land. We will present the results and discuss their implications in regional climatic effects.

Lau, William K. M.↗
NASA NTRS2002

Numerical Simulation of the Large-Scale North American Monsoon Water Sources

A general circulation model (GCM) that includes water vapor tracer (WVT) diagnostics is used to delineate the dominant sources of water vapor for precipitation during the North American monsoon. A 15-year model simulation carried out with one-degree horizontal resolution and time varying sea surface temperature is able to produce reasonable large-scale features of the monsoon precipitation. Within the core of the Mexican monsoon, continental sources provide much of the water for precipitation. Away from the Mexican monsoon (eastern Mexico and Texas), continental sources generally decrease with monsoon onset. Tropical Atlantic Ocean sources of water gain influence in the southern Great Plains states where the total precipitation decreases during the monsoon onset. Pacific ocean sources do contribute to the monsoon, but tend to be weaker after onset. Evaluating the development of the monsoons, soil water and surface evaporation prior to monsoon onset do not correlate with the eventual monsoon intensity. However, the most intense monsoons do use more local sources of water than the least intense monsoons, but only after the onset. This suggests that precipitation recycling is an important factor in monsoon intensity.

Bosilovich, Michael G.↗
NASA NTRS2003

Two Strategies for Microbial Production of an Industrial Enzyme-Alpha-Amylase

Extremophiles are microorganisms that thrive in, from an anthropocentric view, extreme environments including hot springs, soda lakes and arctic water. This ability of survival at extreme conditions has rendered extremophiles to be of interest in astrobiology, evolutionary biology as well as in industrial applications. Of particular interest to the biotechnology industry are the biological catalysts of the extremophiles, the extremozymes, whose unique stabilities at extreme conditions make them potential sources of novel enzymes in industrial applications. There are two major approaches to microbial enzyme production. This entails enzyme isolation directly from the natural host or creating a recombinant expression system whereby the targeted enzyme can be overexpressed in a mesophilic host. We are employing both methods in the effort to produce alpha-amylases from a hyperthermophilic archaeon (Thermococcus) isolated from a hydrothermal vent in the Atlantic Ocean, as well as from alkaliphilic bacteria (Bacillus) isolated from a soda lake in Tanzania. Alpha-amylases catalyze the hydrolysis of internal alpha-1,4-glycosidic linkages in starch to produce smaller sugars. Thermostable alpha-amylases are used in the liquefaction of starch for production of fructose and glucose syrups, whereas alpha-amylases stable at high pH have potential as detergent additives. The alpha-amylase encoding gene from Thermococcus was PCR amplified using carefully designed primers and analyzed using bioinformatics tools such as BLAST and Multiple Sequence Alignment for cloning and expression in E.coli. Four strains of Bacillus were grown in alkaline starch-enriched medium of which the culture supernatant was used as enzyme source. Amylolytic activity was detected using the starch-iodine method.

Bernhardsdotter, Eva C. M. J.↗