Deficiency of α Calcitonin-gene-related peptide impairs peri-implant angiogenesis and osseointegration via suppressive vasodilative activity
Highlights: • Peri-implant neovasculature in metaphyseal femur increased and then declined within 14 days during healing process. • Deficiency of αCGRP impeded peri-implant angiogenesis and vascular remodeling alongside with osteogenesis. • The osteogenic and angiogenic effect of αCGRP might be a consequence of its vasodilative activity. Blood supply is essential for the bone healing process to obtain successful osseointegration. α Calcitonin gene-related peptide (αCGRP) is osteoanabolic and is the most potent microvascular vasodilator currently known with validated angiogenic effect in vitro. We previously demonstrated that lentiviral αCGRP overexpression vector system could express the gene effectively to enhance titanium implant osseointegration. In this study, we investigated the effect of αCGRP on peri-implant angiogenesis during healing process in vivo. Based on investigation of blood vessel alteration in the peri-implant region of wild-type mice, we found a reduced angiogenesis alongside a decline in bone-implant contact percentage and bone mass in αCGRP {sup −/−} mice. Overexpression of αCGRP could partly rescue the impairment. Alterations were also detected in three-dimensional vascular structural parameters and expression of bone and vessel related genes. The results showed αCGRP increased vascular volume fraction and mean vessel size, as well as spatially relocated vessels approximate to the region of bone formation. And angiogenic and osteogenic genes were significantly upregulated in the transfection and αCGRP{sup +/+} group. These results suggested that αCGRP played a synergic role in angiogenesis and osseointegration, partly as a consequence of its vasodilative activity.