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DOE OSTI2022

Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition

Rapid microbial growth in the early phase of plant litter decomposition is viewed as an important component of soil organic matter (SOM) formation. However, the microbial taxa and chemical substrates that correlate with carbon storage are not well resolved. The complexity of microbial communities and diverse substrate chemistries that occur in natural soils make it difficult to identify links between community membership and decomposition processes in the soil environment. To identify potential relationships between microbes, soil organic matter, and their impact on carbon storage, we used sand microcosms to control for external environmental factors such as changes in temperature and moisture as well as the variability in available carbon that exist in soil cores. Using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) on microcosm samples from early phase litter decomposition, we found that protein- and tannin-like compounds exhibited the strongest correlation to dissolved organic carbon (DOC) concentration. Proteins correlated positively with DOC concentration, while tannins correlated negatively with DOC. Through random forest, neural network, and indicator species analyses, we identified 42 bacterial and 9 fungal taxa associated with DOC concentration. The majority of bacterial taxa (26 out of 42 taxa) belonged to the phylum Proteobacteria while all fungal taxa belonged to the phylum Ascomycota. Additionally, we identified significant connections between microorganisms and protein-like compounds and found that most taxa (12/14) correlated negatively with proteins indicating that microbial consumption of proteins is likely a significant driver of DOC concentration. This research links DOC concentration with microbial production and/or decomposition of specific metabolites to improve our understanding of microbial metabolism and carbon persistence.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2012

THE PHOTOMETRIC STUDY OF A NEGLECTED NEAR CONTACT BINARY: BS VULPECULAE

We present a detailed study of the close eclipsing binary BS Vulpeculae. Although it is relatively bright (V: 10.9-11.6 mag) and belongs to short-periodic variable stars (P = 0.48 days), it is rather neglected. To perform a thorough period analysis, we collected all available photometric observations that span the time interval of 1898-2010. Observations include archive photographic plate measurements and visually determined eclipse minima timings done in 1979-2003, which were later shown to be biased to accommodate the existing linear ephemeris. Applying our own direct period analysis we found a well-defined shortening of the orbital period of dP/dt = -6.70(17) Multiplication-Sign 10{sup -11} = -2.11(6) ms yr{sup -1}, which implies a continual mass flow from the primary to the secondary component. Using the 2003 version of the Wilson-Van Hamme code, our new complete BV(IR){sub C} light curves were analyzed and the physical parameters of the system were derived. We found that BS Vul is a near contact binary system with the primary component filling its critical Roche lobe. The luminosity enhancement on the left shoulder of the secondary minimum shown in the light curves can be explained as a result of a persistent hot spot on the secondary due to the mass transfer from the primary component to the secondary one and heating the facing hemisphere of the secondary component, which is consistent with our result of period analysis. With the period decrease, BS Vul will evolve toward the contact phase. It is another good observational example as predicted by the theory of thermal relaxation oscillations.

79 ASTRONOMY AND ASTROPHYSICS↗

NASA NTRS2018

Global-scale Evaluation of SMAP, SMOS and ASCAT Soil Moisture Products Using Triple Collocation

Global-scale surface soil moisture products are currently available from multiple remote sensing platforms. Footprint-scale assessments of these products are generally restricted to limited number of densely-instrumented validation sites. However, by taking active and passive soil moisture products together with a third independent soil moisture estimates via land surface modeling, triple collocation (TC) can be applied to estimate the correlation metric of satellite soil moisture products (versus an unknown ground truth) over a quasi-global domain. Here, an assessment of Soil Moisture Active Passive (SMAP), Soil Moisture Ocean Salinity (SMOS) and Advanced SCATterometer (ASCAT) surface soil moisture retrievals via TC is presented. Considering the potential violation of TC error assumptions, the impact of active-passive and satellite-model error cross correlations on the TC-derived inter-comparison results is examined at in situ sites using quadruple collocation analysis. In addition, confidence intervals for the TC-estimated correlation metric are constructed from moving-block bootstrap sampling designed to preserve the temporal persistence of the original (unevenly-sampled) soil moisture time-series. This study is the first to apply TC to obtain a robust global-scale cross-assessment of SMAP, SMOS and ASCAT soil moisture retrieval accuracy in terms of anomaly temporal correlation. Our results confirm the overall advantage of SMAP (with a global average anomaly correlation of 0.76) over SMOS (0.66) and ASCAT (0.63) that has been established in several recent regional, ground-based studies. SMAP is also the best-performing product over the majority of applicable land pixels (52%), although SMOS and ASCAT each shows advantage in distinct geographic regions.

global-scale↗

DOE OSTI2017

The Future of Robotics/Remote Handling Solutions for the Nuclear Industry - 17347

The nuclear industry is seeing a resurgence of development and utilization of advanced integrated systems; fused sensor arrays; autonomous, remote and hybrid operations; and augmented reality display and control system interfaces. A new age of 'robotics' and remote handling technologies. For many years the use of robotics in nuclear applications has been done sparingly due to technical limitations, perceived and real reliability issues, perceived and real risk concerns, and cost. Although robotics have been used successfully on a variety of projects globally, the underuse of this technology, particularly within the nuclear industry both public and private, is overwhelming. As the number of complex, high hazard projects in the nuclear industry increases, the use of robotics for maintenance, inspection, repair, decommissioning, and remediation activities will become increasingly vital in the future. The risks associated with direct human exposure are high and thus eliminating the risk, or managing it to acceptable levels by using robotics/remote handling as well as other technologies is critical to success as more extreme environment operations and tasks are undertaken. Recent and ongoing advances in technology now allows us to safely and effectively remove the direct / in-situ human element from these type of activities. All the while delivering the same or better outcomes when considering the alternatives when limited or no direct / in-situ human intervention would be allowed. The biggest obstacles the nuclear industry faces center on the cost, reliability, maintainability, effectiveness and appropriate uses of robotics/remote handling solutions in a nuclear (radiation and/or contamination) environment. In order to mitigate these concerns, a sound approach to the use of robotics needs to be addressed. This approach must include: 1. A better understanding of what technology is available and how it can be utilized across multiple sectors, e.g. Commercial Off-the-Shelf (COTS) technology modified / adapted for the nuclear sector; 2. How to implement technology in an integrated pragmatic approach that is cost effective by combining technological know-how / subject matter expertise (SME) with the equivalent subject matter expertise in regulatory, operational and maintenance requirements and limitations, such that a more complete set of functional and operational requirements can be jointly developed in the process. 3. The integration of requirements (project, site, regulatory, etc.), risk management (human factors) and technology, i.e. a marriage between political, programmatic, and technological factors. As the development of robotic/remote handling technology progresses and the successful implementation on projects is demonstrated, the better off the industry will be. This paper provides an overview of the fundamental issues facing the nuclear industry with regard to effective and successful technology selection, maturation, deployment, and integrated risk management, and offers suggestions on how we may overcome these persistent issues now and in the future. Robotic systems and solution experts that are focused on the integration of proven technologies - and that are an integrated part of the project team from its onset - are a necessity for successful planning during nuclear operations. The approach to nuclear operations should always be, 'what is the simplest, most effective way we can approach a particular project need?'. Understanding and implementing the right technologies is where the true value will be recognized. The integration of systems, which includes more than just robotics, is key when developing schemes for these types of complex operations. Often times during planning, the remote handling needs of the job are set aside to be evaluated at a later time due to risk concerns and lack of knowledge. The issue is, experts in the area of robotics or remote handling are rarely involved in the upfront planning. As a result, unnecessary constraints, risks, and obstacles are imposed on project teams to deliver. If done intelligently, this should be introduced at the forefront of planning. Using this type of sound approach leads to effective task organization for the end user. By developing an overall philosophy that leverages proven technologies with implementation expertise; i.e. solutions versus products, is a recipe for success. This is the area that is under-appreciated in the industry and one that needs to be recognized on a much broader scale. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2016

Ab initio thermodynamics of magnesium carbonates and hydrates in water-saturated supercritical CO2 and CO2-rich regions

ab initio thermodynamics is used to determine how free energies of magnesium carbonates and hydrates in a CO2–rich environment change with water concentration across a range of temperature and pressure relevant to geochemistry and carbon sequestration (275 K to 375 K and pCO2 of 1 to 210 bar). The methodology is based on first principles density-functional theory (DFT) calculations of the total energies and vibrational entropy of periclase, magnesite, brucite, nesquehonite, and hydromagnesite coupled to the experimental chemical potentials of CO2 and H2O. The impact of water in supercritical CO2 (scCO2), even though less than 1% at saturation, is found to have a significant impact on the stability of hydrated carbonate minerals. Hydromagnesite and nesquehonite are found to be more thermodynamically stable than periclase and brucite in water-saturated scCO2 and hence may be expected to result kinetically from carbonation of these minerals during CO2 sequestration. Under dehydrating conditions nesquehonite destabilizes rapidly, whereas hydromagnesite is much more likely to persist in a CO2-rich environment, consistent with the observations that hydromagnesite is widespread in nature and nesquehonite is relatively rare.

Chaka, Anne M.↗

DOE OSTI2022

It's All About the Envelope: Prioritizing Envelope Upgrades for Electrification of Cold Climate Homes

Building decarbonization via electrification on a clean grid is the most promising climate solution proposed to date for the building sector. In cold climate zones, building electrification will be driven in large part by moving from natural gas space heating to cold climate heat pumps (CCHPs). CCHPs are commercially available today, including economical cold climate air source heat pumps (ccASHPs). But there's one big problem - wide-scale adoption of ccASHPs will dramatically increase winter peak electricity demand, even with the highest efficiency ccASHP products. Furthermore, cold climate space heating loads will drive unprecedented electric system peaks during the lowest periods of renewable generation and are likely to overwhelm existing distribution systems. This scenario is avoidable by coupling electrification with building envelope upgrades to reduce peak heating loads. This paper presents a model, built from home energy audit and research data sets, that quantifies the above challenges. Results demonstrate how weatherization efforts coupled with additional high-performance envelope upgrade measures can prepare the building stock for electrification and show the benefit these measures can bring to future utility operations. Much of this envelope upgrade work is cost-effective, according to conservative cost-benefit testing and program successes to date, and is coupled with substantial non-energy benefits. However, persistent market barriers have made scaling of envelope retrofit work challenging for decades, suggesting additional policy support is required. Lessons learned from previous policy experience, combined with new technology and administrative support, create exciting potential for this decarbonization climate solution.

air sealing↗

DOE OSTI2020

Grain Size Variations in the Murray Formation: Stratigraphic Evidence for Changing Depositional Environments in Gale Crater, Mars

The lowermost exposure of the Murray formation in Gale crater, Mars, was interpreted as sediment deposited in an ancient lake based on data collected by the Curiosity rover. Constraining the stratigraphic extent and duration of this environment has important implications for the paleohydrology of Gale. Insights into early Martian environments and paleofluid flow velocity can be obtained from grain size in rocks. Visual inspection of grain size is not always available for rocks investigated at field sites on Mars due to limited image coverage. But grain sizes can also be estimated from the Gini Index Mean Score, a grain-size proxy that uses point-to-point chemical variations in ChemCam Laser Induced Breakdown Spectroscopy data. Here, new Gini Index Mean Score results indicate that the Murray formation is dominated by mudstones with grains smaller than the spatial resolution of all rover cameras. Intervals of fine to coarse sandstone also are present, some of which are verified using observations of grain size and sedimentary structures in associated images. Overall, results demonstrate that most of the Murray consists of mudstone, suggesting settling of grains from suspension in low energy depositional environments such as lakes. Some of the mudstones contain desiccation cracks indicating periods of drying with a lowering of lake water level. However, beds and lenses of cross-bedded sandstones are common at specific intervals, suggesting episodes of fluvial and possibly eolian deposition. The persistence of lacustrine deposits interspersed with fluvial deposits suggests that liquid water was sustained on the Martian surface for tens of thousands to millions of years.

58 GEOSCIENCES↗

DOE OSTI2024

Indicators of Global Climate Change 2023: annual update of key indicators of the state of the climate system and human influence

Intergovernmental Panel on Climate Change (IPCC) assessments are the trusted source of scientific evidence for climate negotiations taking place under the United Nations Framework Convention on Climate Change (UNFCCC). Evidence-based decision-making needs to be informed by up-to-date and timely information on key indicators of the state of the climate system and of the human influence on the global climate system. However, successive IPCC reports are published at intervals of 5–10 years, creating potential for an information gap between report cycles. We follow methods as close as possible to those used in the IPCC Sixth Assessment Report (AR6) Working Group One (WGI) report. We compile monitoring datasets to produce estimates for key climate indicators related to forcing of the climate system: emissions of greenhouse gases and short-lived climate forcers, greenhouse gas concentrations, radiative forcing, the Earth's energy imbalance, surface temperature changes, warming attributed to human activities, the remaining carbon budget, and estimates of global temperature extremes. The purpose of this effort, grounded in an open-data, open-science approach, is to make annually updated reliable global climate indicators available in the public domain. As they are traceable to IPCC report methods, they can be trusted by all parties involved in UNFCCC negotiations and help convey wider understanding of the latest knowledge of the climate system and its direction of travel. The indicators show that, for the 2014–2023 decade average, observed warming was 1.19 [1.06 to 1.30] °C, of which 1.19 [1.0 to 1.4] °C was human-induced. For the single-year average, human-induced warming reached 1.31 [1.1 to 1.7] °C in 2023 relative to 1850–1900. The best estimate is below the 2023-observed warming record of 1.43 [1.32 to 1.53] °C, indicating a substantial contribution of internal variability in the 2023 record. Human-induced warming has been increasing at a rate that is unprecedented in the instrumental record, reaching 0.26 [0.2–0.4] °C per decade over 2014–2023. This high rate of warming is caused by a combination of net greenhouse gas emissions being at a persistent high of 53±5.4 Gt CO 2 e yr -1 over the last decade, as well as reductions in the strength of aerosol cooling. Despite this, there is evidence that the rate of increase in CO 2 emissions over the last decade has slowed compared to the 2000s, and depending on societal choices, a continued series of these annual updates over the critical 2020s decade could track a change of direction for some of the indicators presented here.

54 ENVIRONMENTAL SCIENCES↗

NASA NTRS2018

Assessing C2 Communications for UAS Traffic Management

The National Aeronautics and Space Administration's (NASA) Unmanned Aircraft Systems (UAS) Traffic Management (UTM) project works to develop tools and technologies essential for safely enabling civilian low-altitude UAS operations. Currently there is no established infrastructure to enable and safely manage the widespread use of low-altitude airspace and UAS operations, regardless of the type of UAS. The UTM technical challenge will develop comprehensive and validated airspace operations and integration requirements to safely enable large-scale persistent access to visual line of sight and autonomous beyond visual line of sight small UAS in low-altitude airspace. Within the UTM project, a number of communications technologies to support UTM command and control (C2) are under investigation. In particular, commercial networked cellular systems are being tested and assessed for their ability to meet the reliability, scalability, cybersecurity and redundancy required. NASA Glenn Research Center is studying some of the aspects of employing such networks for UTM C2 communications. This includes the development of a test platform for sensing and characterizing the airborne C2 communications environment at various altitudes and in various terrains and topologies, measuring such aspects as received signal strength and interference. System performance aspects such as latency in the link, handover performance, packet error loss rate, drop outs, coverage gaps and other aspects impacting UTM operation will also be assessed. In this paper we explore some of the C2 approaches being proposed and demonstrated in the UTM project, the reliability, availability and other general C2 performance requirements, and approaches to evaluating and analyzing UTM C2 links based on commercial cellular networks.

air traffic control↗

NASA NTRS2018

Assessing C2 Communications

aircraft communications↗

DOE OSTI2022

Legacies of precipitation influence primary production in Panicum virgatum

Precipitation is a key driver of primary production worldwide, but primary production does not always track year-to-year variation in precipitation linearly. Instead, plant responses to changes in precipitation may exhibit time lags, or legacies of past precipitation. Legacies can be driven by multiple mechanisms, including persistent changes in plant physiological and morphological traits and changes to the physical environment, such as plant access to soil water. Here, we used three precipitation manipulation experiments in central Texas, USA to evaluate the magnitude, duration, and potential mechanisms driving precipitation legacies on aboveground primary production of the perennial C 4 grass, Panicum virgatum. Specifically, we performed a rainout shelter study, where eight genotypes grew under different precipitation regimes; a transplant study, where plants that had previously grown in a rainout shelter under different precipitation regimes were moved to a common environment; and a mesocosm study, where the effect of swapping precipitation regime was examined with a single genotype. Across these experiments, plants previously grown under wet conditions generally performed better than expected when exposed to drought. Panicum virgatum exhibited stronger productivity legacies of past wet years on current-year responses to drought than of past dry years on current-year responses to wet conditions. Additionally, previous year tiller counts, a proxy for meristem availability, were important in determining legacy effects on aboveground production. As climate changes and precipitation extremes—both dry and wet—become more common, these results suggest that populations of P. virgatum may become less resilient.

54 ENVIRONMENTAL SCIENCES↗

NASA NTRSDate not supplied

Automated Tracking of Shallow Maritime Clouds on Geostationary Imagery to Extract Lifecycle Characteristics

Shallow moist convection is ubiquitous throughout the tropics and represents a key player in boundary layer processes. Satellites have provided many statistics on shallow clouds, such as size, structure, and geographical coverage, from static views of recurring cloud fields. But determining why certain cloud features appear and persist for different periods requires a time-evolving view of their behaviors. Geostationary satellites provide a unique opportunity to follow the time evolution of individual convective features, given their enhanced spatial and temporal sampling. A cloud-tracking tool was developed to identify properties of cloud lifecycle from the NASA Cloud, Aerosol, and Monsoon Processes Philippines Experiment (CAMP2EX) field campaign of 2019. The mission conducted intensive sampling of shallow cumulus in the West Pacific Ocean, in tandem with Rapid Scan imagery from the Advanced Himawari Imager (AHI) on the Japan Meteorological Agency’s (JMA) Himawari-8 satellite. Shallow cumulus was segmented according to thresholds in 0.5-km visible reflectance and with blurring techniques. Despite being limited to daytime hours, the segmentations yielded the best resolution possible for capturing cloud initiation and decay. The tracking procedure is based on a computer vision package that includes Kalman filters for motion prediction, object overlap search, and the Hungarian (or Kuhn-Munkres) matching algorithm for track designation. AHI radiances available within the tracked cloud boundaries are assembled to form individual spectral histories. The resulting catalog provides thousands of cloud histories for domains measuring only a few degrees in latitude and longitude. We present an overview of the cloud-tracking tool, strategies to identify development stages from cloud tracks, and preliminary results that document cumulus lifecycle properties from satellite. The application of AHI 0.5-km reflectance has both strengths and limitations when attempting to track lifecycles of the smallest resolvable clouds. We show that by aggregating cloud tracks from a few case studies of CAMP2EX, we can discern differences in cloud lifetime and development according to ensembles selected from areas of interest. The results demonstrate an ability to quantify lifetimes and assess rates of change in cloud characteristics that are likely controlled by the surrounding environment and meteorology.

Cloud Tracking↗

DOE OSTI2022

Early diagenetic processes in an iron-dominated marine depositional system

The early diagenetic interplay between reactive iron, sulfur, and organic matter in the bathymetrically isolated Santa Monica Basin (SMB) sediments are investigated in this study. Here, we explore solid-phase and porewater profiles from the basin, supplemented with a transect from 71 to 907 m water depth that includes oxygenated (>60 μM O 2 ) bottom waters near the coast and oxygen-deficient waters (~4 µM O 2 ) in the basin. The geochemical data of the basin sediments are further scrutinized by means of reactive transport modeling. The results show that the basin sediments do not follow the traditional geochemical signatures of oxygen-deficient settings. A lack of dissolved sulfide accumulation and sulfurized iron persists despite the sediments being deposited under reducing conditions (without bioturbation/bioirrigation), strong organic carbon input (TOC up to 5.0 wt%), and active dissimilatory sulfate reduction. Not only did we find an exceptional enrichment in highly reactive Fe in the surface sediments (~45 % of total Fe), but the enrichment of reactive Fe, including ferrihydrite, persists downcore and coexists with high levels of dissolved Fe. The enhanced preservation of Fe oxides and lack of iron-sulfide precipitation is in part explained by detection via Mössbauer spectra of iron oxides bounded to organic matter (Fe[III]-OM coprecipitates). The modeled Fe budget shows that most of the Fe oxides in the surface sediments are internally recycled by upward diffusion and subsequent oxidation of Fe 2+ . Sulfide oxidation coupled to Fe reduction effectively precludes sulfide accumulation while enhancing build-up of dissolved Fe, fueling the Fe cycle within the first 5 cm depth. Continuous reoxidation of Fe 2+ enhances the formation of Fe(III)-OM coprecipitates, limiting the amount of reactive organic matter. In the unavailability of labile organic matter, other than within the uppermost layers, the organic-rich sediment profiles are dominated by Fe cycling that limits the production and preservation of sulfides and enhances the preservation of Fe oxides and organic carbon. Finally, this study highlights key local controls on Fe availability in marginal basins and describes an intricate biogeochemical C-Fe-S cycling in modern and possibly ancient marine systems with important implications for Fe availability in the marine realm.

58 GEOSCIENCES↗

DOE OSTI2020

Persistent minimal sequences of SARS-CoV-2

Abstract Motivation Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 14 million cases and more than half million deaths. Given the absence of implemented therapies, new analysis, diagnosis and therapeutics are of great importance. Results Analysis of SARS-CoV-2 genomes from the current outbreak reveals the presence of short persistent DNA/RNA sequences that are absent from the human genome and transcriptome (PmRAWs). For the PmRAWs with length 12, only four exist at the same location in all SARS-CoV-2. At the gene level, we found one PmRAW of size 13 at the Spike glycoprotein coding sequence. This protein is fundamental for binding in human ACE2 and further use as an entry receptor to invade target cells. Applying protein structural prediction, we localized this PmRAW at the surface of the Spike protein, providing a potential targeted vector for diagnostics and therapeutics. In addition, we show a new pattern of relative absent words (RAWs), characterized by the progressive increase of GC content (Guanine and Cytosine) according to the decrease of RAWs length, contrarily to the virus and host genome distributions. New analysis shows the same property during the Ebola virus outbreak. At a computational level, we improved the alignment-free method to identify pathogen-specific signatures in balance with GC measures and removed previous size limitations. Availability and implementation https://github.com/cobilab/eagle. Supplementary information Supplementary data are available at Bioinformatics online.

Pratas, Diogo↗

NASA NTRS2011

Component-Level Electronic-Assembly Repair (CLEAR) Analysis of the Problem Reporting and Corrective Action (PRACA) Database of the International Space Station On-Orbit Electrical Systems

The NASA Constellation Program is investigating and developing technologies to support human exploration of the Moon and Mars. The Component-Level Electronic-Assembly Repair (CLEAR) task is part of the Supportability Project managed by the Exploration Technology Development Program. CLEAR is aimed at enabling a flight crew to diagnose and repair electronic circuits in space yet minimize logistics spares, equipment, and crew time and training. For insight into actual space repair needs, in early 2008 the project examined the operational experience of the International Space Station (ISS) program. CLEAR examined the ISS on-orbit Problem Reporting and Corrective Action database for electrical and electronic system problems. The ISS has higher than predicted reliability yet, as expected, it has persistent problems. A goal was to identify which on-orbit electrical problems could be resolved by a component-level replacement. A further goal was to identify problems that could benefit from the additional diagnostic and test capability that a component-level repair capability could provide. The study indicated that many problems stem from a small set of root causes that also represent distinct component problems. The study also determined that there are certain recurring problems where the current telemetry instrumentation and built-in tests are unable to completely resolve the problem. As a result, the root cause is listed as unknown. Overall, roughly 42 percent of on-orbit electrical problems on ISS could be addressed with a component-level repair. Furthermore, 63 percent of on-orbit electrical problems on ISS could benefit from additional external diagnostic and test capability. These results indicate that in situ component-level repair in combination with diagnostic and test capability can be expected to increase system availability and reduce logistics. The CLEAR approach can increase the flight crew s ability to act decisively to resolve problems while reducing dependency on Earth-supplied logistics for future Constellation Program missions.

Oeftering, Richard C.↗

NASA NTRS2012

Genetic Inventory Task Final Report

Contaminant terrestrial microbiota could profoundly impact the scientific integrity of extraterrestrial life-detection experiments. It is therefore important to know what organisms persist on spacecraft surfaces so that their presence can be eliminated or discriminated from authentic extraterrestrial biosignatures. Although there is a growing understanding of the biodiversity associated with spacecraft and cleanroom surfaces, it remains challenging to assess the risk of these microbes confounding life-detection or sample-return experiments. A key challenge is to provide a comprehensive inventory of microbes present on spacecraft surfaces. To assess the phylogenetic breadth of microorganisms on spacecraft and associated surfaces, the Genetic Inventory team used three technologies: conventional cloning techniques, PhyloChip DNA microarrays, and 454 tag-encoded pyrosequencing, together with a methodology to systematically collect, process, and archive nucleic acids. These three analysis methods yielded considerably different results: Traditional approaches provided the least comprehensive assessment of microbial diversity, while PhyloChip and pyrosequencing illuminated more diverse microbial populations. The overall results stress the importance of selecting sample collection and processing approaches based on the desired target and required level of detection. The DNA archive generated in this study can be made available to future researchers as genetic-inventory-oriented technologies further mature.

microbial diversity↗

DOE OSTI2014

Studies of the transverse structure of the nucleon at JLab

Since the earliest measurements in the '70, hadronic physics deals with a number of surprising phenomena that cannot be explained in the framework of perturbative QCD. Examples are the small fraction of the proton spin carried by the valence quark spins, the persistence at high energies of single spin asymmetries and azimuthal asymmetries in unpolarized processes. It is now believed that the answer to these questions may come from the transverse motion of partons inside the nucleon, which is encoded in the Transverse Momentum Dependent (TMD) Parton Distribution Functions. Among the large variety of processes that can be described in terms of TMDs, a major role is played by Semi-Inclusive Deep Inelastic Scattering (SIDIS) reactions, in which, together with the scattered electron, one or more hadrons are detected in the final state. Single and Double Spin Asymmetries are the experimental observables sensitive to TMDs. The identification of the final hadrons allows the tagging of the quark involved in the reaction at the parton level, and then the flavor separation of the relevant TMDs. SIDIS reactions are studied at Jefferson Laboratories since many years and are one of the main items in the physics program after the upgrade of the CEBAF accelerator. The large amount of new data that will be available in few years calls for the implementation of new tools, such as multidimensional analyses and refined techniques of TMDs extraction from the experimental asymmetries. In this talk, the more recent results obtained at 6 GeV will be shown and the future measurements will be discussed.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

DOE OSTI2014

Birth, life, and death of a solar coronal plume

We analyze a solar polar-coronal-hole (CH) plume over its entire ≈40 hr lifetime, using high-resolution Solar Dynamic Observatory Atmospheric Imaging Assembly (AIA) data. We examine (1) the plume's relationship to a bright point (BP) that persists at its base, (2) plume outflows and their possible contribution to the solar wind mass supply, and (3) the physical properties of the plume. We find that the plume started ≈2 hr after the BP first appeared and became undetectable ≈1 hr after the BP disappeared. We detected radially moving radiance variations from both the plume and from interplume regions, corresponding to apparent outflow speeds ranging over ≈(30-300) km s{sup –1} with outflow velocities being higher in the 'cooler' AIA 171 Å channel than in the 'hotter' 193 Å and 211 Å channels, which is inconsistent with wave motions; therefore, we conclude that the observed radiance variations represent material outflows. If they persist into the heliosphere and plumes cover ≈10% of a typical CH area, these flows could account for ≈50% of the solar wind mass. From a differential emission measure analysis of the AIA images, we find that the average electron temperature of the plume remained approximately constant over its lifetime, at T {sub e} ≈ 8.5 × 10{sup 5} K. Its density, however, decreased with the age of the plume, being about a factor of three lower when the plume faded compared to when it was born. We conclude that the plume died due to a density reduction rather than to a temperature decrease.

79 ASTRONOMY AND ASTROPHYSICS↗