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At least 631 records · Page 35

Muon detection studied by pulse-height energy analysis: Novel converter arrangements

Muons are conventionally measured by a plastic scintillator–photomultiplier detector. Muons from processes in ultra-dense hydrogen H(0) are detected here by a novel type of converter in front of a photomultiplier. The muon detection yield can be increased relative to that observed with a plastic scintillator by at least a factor of 100, using a converter of metal, semiconductor (Ge), or glass for interaction with the muons penetrating through the metal housing of the detector. This detection process is due to transient formation of excited nuclei by the well-known process of muon capture, giving beta decay. The main experimental results shown here are in the form of beta electron energy spectra detected directly by the photomultiplier. Events which give a high-energy tail in the energy spectra are probably due to gamma photons from the muons. Sharp and intense x-ray peaks from a muonic aluminium converter or housing material are observed. The detection conversion in glass and Ge converters has a time constant of the order of many minutes to reach the final conversion level, while the process in metal converters is stabilized faster. The time constants are not due to lifetimes of the excited nuclei or neutrons but are due to internal charging in the insulating converter material. Interaction of this charging with the high voltage in the photomultiplier is observed.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Variable-spectrum mode control of high poloidal beta discharges

DIII-D experiments demonstrate that high pressure, broad current profile equilibria can be accessed in the high poloidal beta regime by optimizing the MHD mode control poloidal spectrum. A novel, variable spectrum (VS) magnetic feedback scheme implemented using the DIII-D internal non-axisymmetric coils (I-coils) facilitated access to reduced internal inductance $l$ i operation above the no-wall beta limit compared with both no feedback and fixed spectrum feedback. In addition, the VS feedback helped avoid beta collapses caused by marginally unstable resistive wall mode activity. The lower and upper I-coil rows were configured in two independent feedback loops, allowing the feedback field's poloidal spectrum to vary and track changes in the plasma mode structure as the edge safety factor q 95 varied from 11 to 6 during the discharges. The q 95 dependence of the measured phase difference between the lower and upper I-coil rows during VS feedback is qualitatively compatible with ideal MHD simulations of the least-stable plasma kink mode and with plasma response simulations that included kinetic modifications to ideal MHD. The VS feedback approach is a straightforward way to improve resilience to variations in mode structure that occur as plasma parameters change. The demonstrated expansion of the operating space to lower $l$ i is expected to improve the coupling of the plasma kink mode to external fields and beneficial wall eddy currents, and is compatible with high bootstrap fraction operation.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

A search for molecular hydrogen fluorescence near 100 km

The fluorescence of H2 in the Lyman band system, excited by solar extreme ultraviolet radiation, provides a means for the optical detection of H2 in the upper atmosphere. In particular, the Ly beta line of hydrogen is nearly degenerate with the (6,0) P1 transition, and absorption in this line produces fluorescence in the v-prime = 6 progression, principally at 1265, 1366, 1462 and 1608 A. Absorption by O2 rapidly attenuates the Ly beta from an overhead sun below 100 km and also significantly attenuates the fluorescent radiation. Far-ultraviolet dayglow spectra from 1130 to 1510 A obtained from an Aerobee rocket experiment on 11 December 1972 give an upper limit for any H2 emission which is a factor of 5 higher than expected according to recent hydrogen models.

Feldman, P. D.↗

Targeted gene expression without a tissue-specific promoter: creating mosaic embryos using laser-induced single-cell heat shock

We have developed a method to target gene expression in the Drosophila embryo to a specific cell without having a promoter that directs expression in that particular cell. Using a digitally enhanced imaging system to identify single cells within the living embryo, we apply a heat shock to each cell individually by using a laser microbeam. A 1- to 2-min laser treatment is sufficient to induce a heat-shock response but is not lethal to the heat-shocked cells. Induction of heat shock was measured in a variety of cell types, including neurons and somatic muscles, by the expression of beta-galactosidase from an hsp26-lacZ reporter construct or by expression of a UAS target gene after induction of hsGAL4. We discuss the applicability of this technique to ectopic gene expression studies, lineage tracing, gene inactivation studies, and studies of cells in vitro. Laser heat shock is a versatile technique that can be adapted for use in a variety of research organisms and is useful for any studies in which it is desirable to express a given gene in only a distinct cell or clone of cells, either transiently or constitutively, at a time point of choice.

Non-NASA Center↗

Structural integration in hypoxia-inducible factors

The hypoxia-inducible factors (HIFs) coordinate cellular adaptations to low oxygen stress by regulating transcriptional programs in erythropoiesis, angiogenesis and metabolism. These programs promote the growth and progression of many tumours, making HIFs attractive anticancer targets. Transcriptionally active HIFs consist of HIF-alpha and ARNT (also called HIF-1 beta) subunits. Here we describe crystal structures for each of mouse HIF-2 alpha-ARNT and HIF-1 alpha-ARNT heterodimers in states that include bound small molecules and their hypoxia response element. A highly integrated quaternary architecture is shared by HIF-2 alpha-ARNT and HIF-1 alpha-ARNT, wherein ARNT spirals around the outside of each HIF-alpha subunit. Five distinct pockets are observed that permit small-molecule binding, including PAS domain encapsulated sites and an interfacial cavity formed through subunit heterodimerization. The DNA-reading head rotates, extends and cooperates with a distal PAS domain to bind hypoxia response elements. HIF-alpha mutations linked to human cancers map to sensitive sites that establish DNA binding and the stability of PAS domains and pockets.

Wu, Dalei↗

On the origin of biological chirality via natural beta-decay

An hypothesis to account for the chirality (handedness) of some biological molecules is given. Experimental evidence suggests that longitudinally polarized electrons having the chirality of terrestrial beta-decay electrons remove dextro-leucine from a racemic mixture. If, by a similar mechanism, the terrestrial environment provided more levo- than dextro-amino acids, that would account for the chirality now observed in organic molecules. An isotope of potassium has been proposed as the natural beta-emitter responsible for biomolecular chirality; however, Carbon 14 may be an even more plausible candidate. Ready availability of the carbon isotope in the terrestrial environment of 4.5 aeons ago, and the role of leucine in protein synthesis indicate that these two agents may have been chief factors in the evolution of biomolecular chirality. Suggestions for further research in this area are made.

Noyes, H. P.↗

Magnetic structure of the low beta, quasi-perpendicular shock

The structure of the low-beta quasi-perpendicular shock is examined in view of ISEE 1 and 2 magnetic field measurements. An analysis of shock overshoots indicates that the strength of the overshoots of low-beta, quasi-perpendicular shocks increases as the ratio of the Mach number to the first critical Mach number increases. Wave analysis indicates that the power of the downstream waves also increases as a function of this ratio of criticality. The thickness of the shock is a factor of 1-2 times greater than a precursor wavelength, countering the conjecture that the shock is the last amplified cycle of the precursor wave.

Farris, M. H.↗

Discovery of Be/x-ray stars in two supernova remnants in the Small Magellanic Cloud

We present ROSAT high resolution x-ray images of two previously cataloged supernova remnants in the Small Magellanic Cloud (SMC): 0101-72.4 and 0104-72.3. These remnants were known to show optical, x-ray, and radio emission based on earlier observations: the present data show the first evidence for arcsecond-scale x-ray structure. There is no diffuse x-ray emission associated with the optically emitting shell in 0101-72.4; we set a 3 sigma upper limit of 7 x 10(exp 34) erg s(exp -1) on the 0.02-2 keV luminosity from the region. The x-ray emission comes instead from a weak pointlike object near the limb of the remnant. Optical observations of this source reveal a m(sub v) = 14.8 blue star with H alpha and H beta in emission; we identify this as a Be star in the SMC. No evidence for variability down to time scales of about 1 s was found in the ROSAT data; however, a comparison of the ROSAT and Einstein fluxes indicates possible long term variability by a factor of approximately 2 over several years. The other SNR, 0104-72.3, also contains a pointlike x-ray source with a blue optical counterpart (m(sub v) = 16.7) and H alpha emission. We tentatively identify this as a Be star as well. In addition to the point source there is weak diffuse x-ray emission from 0104-72.3 (L(sub x) approximately 1.4 x 10(exp 35) erg s(exp -1)), but the remnant's appearance in the x-ray band is considerably different from that in either the radio or optical band. We argue for a physical association between the supernova remnants (SNRs) and Be/x-ray stars. A large space velocity (greater than or approximately 100 km s(exp -1)) for the Be/x-ray binaries is required if the explosions that produced the remnants also formed the neutron stars in the binaries. Alternatively, the associations could be the result of common membership in OB associations in the SMC.

Hughes, John P.↗

Structural and Functional Analysis of the Interaction Between the Nucleoporin Nup98 and the mRNA Export Facto Rae1

The export of mRNAs is a multistep process, involving the packaging of mRNAs into messenger ribonucleoprotein particles (mRNPs), their transport through nuclear pore complexes, and mRNP remodeling events prior to translation. Ribonucleic acid export 1 (Rae1) and Nup98 are evolutionarily conserved mRNA export factors that are targeted by the vesicular stomatitis virus matrix protein to inhibit host cell nuclear export. Here, we present the crystal structure of human Rae1 in complex with the Gle2-binding sequence (GLEBS) of Nup98 at 1.65 {angstrom} resolution. Rae1 forms a seven-bladed {beta}-propeller with several extensive surface loops. The Nup98 GLEBS motif forms an {approx}50-{angstrom}-long hairpin that binds with its C-terminal arm to an essentially invariant hydrophobic surface that extends over the entire top face of the Rae1 {beta}-propeller. The C-terminal arm of the GLEBS hairpin is necessary and sufficient for Rae1 binding, and we identify a tandem glutamate element in this arm as critical for complex formation. The Rae1 {center_dot} Nup98{sup GLEBS} surface features an additional conserved patch with a positive electrostatic potential, and we demonstrate that the complex possesses single-stranded RNA-binding capability. Together, these data suggest that the Rae1 {center_dot} Nup98 complex directly binds to the mRNP at several stages of the mRNA export pathway.

59 BASIC BIOLOGICAL SCIENCES↗

Ultra-low level radon assays in gases

The SuperNEMO experiment aims to search for the neutrinoless double beta decay (0νβ β) to T{sub 1{sub /{sub 2}}}(0ν) > 10{sup 26} years, this corresponds to an effective neutrino mass of 50-100 meV. The extremely rare event rate means the minimisation of background is of critical concern. The stringent strategy instigated to ensure detector radiopurity is outlined here for all construction materials. In particular the large R&D programme undertaken to reach the challengingly low level of radon, < 0.15 mBq/m{sup 3}, required inside the SuperNEMO gaseous tracker will be detailed. This includes an experiment designed to measure radon diffusion through various materials. A “Radon Concentration Line” (RnCL) was developed to be used in conjunction with a state-of-the-art radon detector in order to achieve world leading sensitivity to {sup 222}Rn content in large gas volumes at the level of a few µBq/m{sup 3}. A radon purification system was developed and installed which has demonstrated radon suppression by several orders of magnitude depending on the carrier gas. This apparatus has now been commissioned and measurements of cylindered gas have been made to confirm radon suppression by a factor 20 when using nitrogen as the carrier gas. The results from measurements of radon content in various gases, used inside SuperNEMO, using the RnCL will be presented.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Comprehensive review of 2 β decay half-lives

Here, the double-beta (2 β )-decay is the rarest nuclear physics process, and its experimental half-lives (T 1/2 ) exceed the age of the Universe from nine to fourteen orders of magnitude. Double-beta decay was observed, and its half-life was measured in 14 parent nuclei using direct, radiochemical, and geochemical methods. The decay observables are analyzed using the Evaluated Nuclear Structure Data File (ENSDF) procedures, and the recommended T 1/2 were deduced. Using the calculated values of phase factors, the effective nuclear matrix elements were extracted and compared with available data. Thousands of theoretical and experimental works have been dedicated to these topics in the last 85 years, and we present two data sets of recommended values to encapsulate the results.

2β-decay↗

Comprehensive Review of 2$β$ Decay Half-Lives

The double-beta (2β)-decay is the rarest nuclear physics process, and its experimental half-lives (T 1/2 ) exceed the age of the Universe from nine to fourteen orders of magnitude. Double-beta decay was observed, and its half-life was measured in 14 parent nuclei using direct, radiochemical, and geochemical methods. The decay observables are analyzed using the Evaluated Nuclear Structure Data File (ENSDF) procedures, and the recommended T 1/2 were deduced. Using the calculated values of phase factors, the effective nuclear matrix elements were extracted and compared with available data. Thousands of theoretical and experimental works have been dedicated to these topics in the last 85 years, and we present two data sets of recommended values to encapsulate the results.

2β-decay↗

Effects of substrate temperature on properties of NbNx films grown on Nb by pulsed laser deposition

NbN{sub x} films were deposited on Nb substrate using pulsed laser deposition. The effects of substrate deposition temperature, from room temperature to 950 C, on the preferred orientation, phase, and surface properties of NbN{sub x} films were studied by X-ray diffraction, atomic force microscopy, and electron probe micro analyzer. We find that the substrate temperature is a critical factor in determining the phase of the NbN{sub x} films. For a substrate temperature up to 450 C the film showed poor crystalline quality. With temperature increase the film became textured and for a substrate temperature of 650-850 C, mix of cubic {delta}-NbN and hexagonal phases ({beta}-Nb{sub 2}N + {delta}'-NbN) were formed. Films with a mainly {beta}-Nb{sub 2}N hexagonal phase were obtained at deposition temperature above 850 C. The c/a ratio of {beta}-Nb{sub 2}N hexagonal shows an increase with increased nitrogen content. The surface roughness of the NbN{sub x} films increased as the temperature was raised from 450 to 850 C.

36 MATERIALS SCIENCE↗

Effects of oxidative stress on hyperglycaemia-induced brain malformations in a diabetes mouse model

Pregestational diabetes mellitus (PGDM) enhances the risk of fetal neurodevelopmental defects. However, the mechanism of hyperglycaemia-induced neurodevelopmental defects is not fully understood. In this study, several typical neurodevelopmental defects were identified in the streptozotocin-induced diabetes mouse model. The neuron-specific class III beta-tubulin/forkhead box P1-labelled neuronal differentiation was suppressed and glial fibrillary acidic protein-labelled glial cell lineage differentiation was slightly promoted in pregestational diabetes mellitus (PGDM) mice. Various concentrations of glucose did not change the U87 cell viability, but glial cell line-derived neurotrophic factor expression was altered with varying glucose concentrations. Mouse maternal hyperglycaemia significantly increased Tunel{sup +} apoptosis but did not dramatically affect PCNA{sup +} cell proliferation in the process. To determine the cause of increased apoptosis, we determined the SOD activity, the expression of Nrf2 as well as its downstream anti-oxidative factors NQO1 and HO1, and found that all of them significantly increased in PGDM fetal brains compared with controls. However, Nrf2 expression in U87 cells was not significantly changed by different glucose concentrations. In mouse telencephalon, we observed the co-localization of Tuj-1 and Nrf2 expression in neurons, and down-regulating of Nrf2 in SH-SY5Y cells altered the viability of SH-SY5Y cells exposed to high glucose concentrations. Taken together, the data suggest that Nrf2-modulated antioxidant stress plays a crucial role in maternal hyperglycaemia-induced neurodevelopmental defects. - Highlights: • Typical neurodevelopmental defects could be observed in STZ-treated mouse fetuses. • Nrf2 played a crucial role in hyperglycaemia-induced brain malformations. • The effects of hyperglycaemia on neurons and glia cells were not same.

60 APPLIED LIFE SCIENCES↗

Visser's massive graviton bimetric theory revisited

A massive gravity theory was proposed by Visser in the late 1990s. This theory, based on a background metric b{sub {alpha}{beta}} and on an usual dynamical metric g{sub {alpha}{beta}} has the advantage of being free of ghosts as well as discontinuities present in other massive theories proposed in the past. In the present investigation, the equations of Visser's theory are revisited with particular care on the related conservation laws. It will be shown that a multiplicative factor is missing in the graviton tensor originally derived by Visser, which has no incidence on the weak field approach but becomes important in the strong field regime when, for instance, cosmological applications are considered. In this case, contrary to some previous claims found in the literature, we conclude that a nonstatic background metric is required in order to obtain a solution able to mimic the {Lambda}CDM cosmology.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

A luminosity model of RHIC gold runs

In this note, we present a luminosity model for RHIC gold runs. The model is applied to the physics fills in 2007 run without cooling, and with the longitudinal cooling applied to one beam only. Having good comparison, the model is used to project a fill with the longitudinal cooling applied to both beams. Further development and possible applications of the model are discussed. To maximize the integrated luminosity, usually the higher beam intensity, smaller longitudinal and transverse emittance, and smaller {beta} are the directions to work on. In past 10 years, the RHIC gold runs have demonstrated a path toward this goal. Most recently, a successful commissioning of the bunched beam stochastic cooling, both longitudinal and transverse, has offered a chance of further RHIC luminosity improvement. With so many factors involved, a luminosity model would be useful to identify and project gains in the machine development. In this article, a preliminary model is proposed. In Section 2, several secondary factors, which are not yet included in the model, are identified based on the RHIC operation condition and experience in current runs. In Section 3, the RHIC beam store parameters used in the model are listed, and validated. In Section 4, the factors included in the model are discussed, and the luminosity model is presented. In Section 5, typical RHIC gold fills without cooling, and with partial cooling are used for comparison with the model. Then a projection of fills with more coolings is shown. In Section 6, further development of the model is discussed.

43 PARTICLE ACCELERATORS↗

Magnetohydrodynamic models of coronal transients in the meridional plane. I - The effect of the magnetic field

The propagation of coronal transients through the lower corona in the meridional plane is studied on the basis of numerical solutions of the time-dependent MHD equations of motion. The importance of the topology of the initial coronal magnetic field is demonstrated by considering two configurations: one which is essentially radial (open), and another which is essentially parallel to the solar surface (closed). The effect of coronal field magnitude is investigated by using values for the beta parameter of 1 and 0.1 at the coronal base. The consequences of including radiative losses and of varying the polytropic index are also considered. The solar event is simulated by a step-function increase in the pressure at the base of an initially hydrostatic atmosphere by a factor of 5, lasting for a period of 5 min.

Steinolfson, R. S.↗

Discovery of a sub-megagauss magnetic white dwarf through spectropolarimetry

A circular polarization survey for weakly magnetized (about 10 kG-1 MG) white dwarfs has been initiated using a newly developed CCD imaging/spectropolarimeter. Among the first two-dozen stars studied, WD 0637 + 477 revealed strong polarization reversals across both H-alpha and H-beta, indicating a mean longitudinal ('effective') field of Be = +349 +/- 19 kG. Corresponding estimates of the mean surface field and dipolar field values are Bs = 950 +/- 100 kG and Bp = 1.2 +/- 0.2 MG. WD 0637 + 477 portrays the weakest field yet found on a magnetic white dwarf by a factor of 2. Given the small survey sample observed to date, statistics on the incidence of magnetic fields in the range 100 kG-1 MG permit a distribution which either increases smoothly with decreasing field strength, or is bimodal, wherein a small percentage of stars possess fields above about 1 MG and the vast majority are essentially nonmagnetized.

Schmidt, Gary D.↗