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At least 631 records · Page 35

Designer membraneless organelles sequester native factors for control of cell behavior

Subcellular compartmentalization of macromolecules increases flux and prevents inhibitory interactions to control biochemical reactions. Inspired by this functionality, we sought to build designer compartments that function as hubs to regulate the flow of information through cellular control systems. Here we report a synthetic membraneless organelle platform to control endogenous cellular activities through sequestration and insulation of native proteins. We engineer and express a disordered protein scaffold to assemble micron size condensates and recruit endogenous clients via genomic tagging with high-affinity dimerization motifs. By relocalizing up to ninety percent of a targeted enzymes to synthetic condensates, we efficiently control cellular behaviors, including proliferation, division, and cytoskeletal organization. Further, we demonstrate multiple strategies for controlled cargo release from condensates to switch cells between functional states. These synthetic organelles offer a powerful and generalizable approach to modularly control cell decision-making in a variety of model systems with broad applications for cellular engineering.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Stochastic Pricing Game for Aggregated Demand Response Considering Comfort Level

In recent years, demand response (DR) has been explored as a fundamental strategy for demand-side management due to its advantages in mediating intermittency of renewable energy generation, load shifting, etc. To engage customers in DR programs, several deterministic price-based DR strategies have been developed and implemented. However, the stochastic weather conditions and occupants' consumption behaviors often make the deterministic solution less robust to uncertainties. In this paper, with the consideration of the uncertainties, a stochastic Stackelberg game is proposed to model the price-demand negotiation between a distributed system operator and load aggregators, where the virtual battery constraints are extracted from the building thermostatically controlled loads (TCLs)‘ characteristics to guarantee comfortable TCLs' levels. Following the negotiation, a priority-based control method is used to allocate the optimal aggregated power DR profile at the building level and track the power signal. Several groups of experiments have demonstrated the effectiveness and robustness of the stochastic solutions.

Chen, Yang↗

Streamlined Approach for Environmental Restoration (SAFER) Plan for Corrective Action Unit 114: Area 25 EMAD Facility Nevada National Security Site, Nevada, (Rev.0, with ROTC 1)

This Streamlined Approach for Environmental Restoration (SAFER) Plan addresses the actions needed to achieve closure for Corrective Action Unit (CAU) 114, Area 25 EMAD Facility, identified in the Federal Facility Agreement and Consent Order (FFACO). CAU 114 comprises the following corrective action sites (CASs) located in Area 25 of the Nevada National Security Site (formerly, the Nevada Test Site): 25-41-03, EMAD Facility; 25-99-23, Manned Control Car (MCC) and Engine Installation Vehicle (EIV); and 25-33-05, Building 3901, Engine Transport System Maintenance Building. This plan provides the methodology for field activities needed to gather the necessary information for closing CAU 114. There is sufficient information and process knowledge from historical documentation and investigations of similar sites regarding the expected nature and extent of potential contaminants to recommend closure of CAU 114 using the SAFER process. Additional information will be obtained by conducting a field investigation before selecting the appropriate corrective actions for CAU 114. It is anticipated that the results of the field investigation and implementation of corrective actions will support a defensible recommendation that no further corrective action is necessary. The purpose of the corrective action investigation will be to document and verify the adequacy of existing information; to affirm the decision for either clean closure, closure in place, or no further action; and to provide sufficient data to implement the corrective action. The actual corrective action selected will be based on characterization activities implemented under this SAFER Plan. If specific conditions or findings fall outside the bounds of the SAFER Plan, the Nevada Division of Environmental Protection (NDEP) will be consulted to determine the path forward before proceeding. Upon completion of SAFER activities, a closure report will be prepared and submitted to NDEP for review and approval. The schedule for completion of the closure report will be established at the FFACO annual meeting.

54 ENVIRONMENTAL SCIENCES↗

Control/structure interaction during Space Station Freedom-Orbiter berthing

The berthing maneuver is essential for the construction and assembly of Space Station Freedom (SSF) and has a direct effect on the SSF assembly build up and SSF/Orbiter operations. The effects of flexible body dynamics coupled with the available control system may impose new requirements on the maneuver. The problem is further complicated by the effect of the SSF control system on the Shuttle Remote Manipulator System (SRMS). These effects will play a major role in the development of operational requirements which need to be identified and validated in order to assure total safety and maneuver execution during SSF construction. This paper presents the results of ongoing studies to investigate the Control/Structure Interaction (CSI) during the berthing operations. The problem is formulated in terms of multi-flex body equations of motion for SSF and the SRMS and on-orbit flight control systems for the SRMS and the SSF, which includes the Control Moment Gyro (CMG) and Reaction Control System (RCS) Attitude Control Systems (ACS). The SSF control system designs are based on the Preliminary Design Review (PDR) version of the Honeywell design. The simulation tool used for the analysis is briefly described and the CSI results are presented for given berthing scenarios.

Hua, Tuyen↗

A Tabletop Tool for Modeling Life Support Systems

This paper describes the development plan for a comprehensive research and diagnostic tool for aspects of advanced life support systems in space-based laboratories. Specifically it aims to build a high fidelity tabletop model that can be used for the purpose of risk mitigation, failure mode analysis, contamination tracking, and testing reliability. We envision a comprehensive approach involving experimental work coupled with numerical simulation to develop this diagnostic tool. It envisions a 10% scale transparent model of a space platform such as the International Space Station that operates with water or a specific matched index of refraction liquid as the working fluid. This allows the scaling of a 10 ft x 10 ft x 10 ft room with air flow to 1 ft x 1 ft x 1 ft tabletop model with water/liquid flow. Dynamic similitude for this length scale dictates model velocities to be 67% of full-scale and thereby the time scale of the model to represent 15% of the full- scale system; meaning identical processes in the model are completed in 15% of the full- scale time. The use of an index matching fluid (fluid that matches the refractive index of cast acrylic, the model material) allows making the entire model (with complex internal geometry) transparent and hence conducive to non-intrusive optical diagnostics. So using such a system one can test environment control parameters such as core flows (axial flows), cross flows (from registers and diffusers), potential problem areas such as flow short circuits, inadequate oxygen content, build up of other gases beyond desirable levels, test mixing processes within the system at local nodes or compartments and assess the overall system performance. The system allows quantitative measurements of contaminants introduced in the system and allows testing and optimizing the tracking process and removal of contaminants. The envisaged system will be modular and hence flexible for quick configuration change and subsequent testing. The data and inferences from the tests will allow for improvements in the development and design of next generation life support systems and configurations.

Ramachandran, N.↗

Building a Simplistic Automatic Extruder: Instrument Development Opportunities for the Laboratory

This work presents an automatic extruder as a research experience for undergraduate students. The system offers a user-friendly approach to preparing vesicles, such as liposomes or polymersomes, with a defined size and polydispersity properties crucial for research in biology and macromolecules. It comprises two syringe pumps connected by a membrane filter. The setup is controlled by software. Compared to manual extrusion, this automated system provides advantages, such as precisely controlled variables. The project describes a tool to enhance undergraduate learning in science and engineering laboratories. Building an automatic extruder serves as a simplified model of a complex industrial process. It offers a clear advantage: automating a well-understood manual extrusion process. To make this project accessible, it is broken down into three manageable tasks: software development, hardware assembly, and testing procedures. This breakdown describes the software created, the hardware components used, and the testing procedures conducted for this project. All project data, including software code, testing data, and procedures, are freely available online. This allows undergraduate students to not only begin their own projects but also contribute to this educational instrument’s ongoing development.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Space Laboratory on a Table Top: A Next Generative ECLSS design and diagnostic tool

This paper describes the development plan for a comprehensive research and diagnostic tool for aspects of advanced life support systems in space-based laboratories. Specifically it aims to build a high fidelity tabletop model that can be used for the purpose of risk mitigation, failure mode analysis, contamination tracking, and testing reliability. We envision a comprehensive approach involving experimental work coupled with numerical simulation to develop this diagnostic tool. It envisions a 10% scale transparent model of a space platform such as the International Space Station that operates with water or a specific matched index of refraction liquid as the working fluid. This allows the scaling of a 10 ft x 10 ft x 10 ft room with air flow to 1 ft x 1 ft x 1 ft tabletop model with water/liquid flow. Dynamic similitude for this length scale dictates model velocities to be 67% of full-scale and thereby the time scale of the model to represent 15% of the full- scale system; meaning identical processes in the model are completed in 15% of the full- scale-time. The use of an index matching fluid (fluid that matches the refractive index of cast acrylic, the model material) allows making the entire model (with complex internal geometry) transparent and hence conducive to non-intrusive optical diagnostics. So using such a system one can test environment control parameters such as core flows (axial flows), cross flows (from registers and diffusers), potential problem areas such as flow short circuits, inadequate oxygen content, build up of other gases beyond desirable levels, test mixing processes within the system at local nodes or compartments and assess the overall system performance. The system allows quantitative measurements of contaminants introduced in the system and allows testing and optimizing the tracking process and removal of contaminants. The envisaged system will be modular and hence flexible for quick configuration change and subsequent testing. The data and inferences from the tests will allow for improvements in the development and design of next generation life support systems and configurations. Preliminary experimental and modeling work in this area will be presented. This involves testing of a single inlet-exit model with detailed 3-D flow visualization and quantitative diagnostics and computational modeling of the system.

Ramachandran, N.↗

Automatic phase control in solar power satellite systems

Various approaches to the problem of generating, maintaining and distributing a coherent, reference phase signal over a large area are suggested, mathematically modeled and analyzed with respect to their ability to minimize: phase build-up, beam diffusion and beam steering phase jitter, cable length, and maximize power transfer efficiency. In addition, phase control configurations are suggested which alleviate the need for layout symmetry.

Lindsey, W. C.↗

Frequency Analysis of Solar PV Power to Enable Optimal Building Load Control

In this paper, we present a flexibility estimation mechanism for buildings’ thermostatically controlled loads (TCLs) to enable the distribution level consumption of the majority of solar photovoltaic (PV) generation by local building TCLs. The local consumption of PV generation provides several advantages to the grid operation as well as the consumers, such as reducing the stress on the distribution network, minimizing voltage fluctuations and two-way power flows in the distribution network, and reducing the required battery storage capacity for PV integration. This would result in increasing the solar PV generation penetration levels. The aims of this study are twofold. First, spectral (frequency) analyses of solar PV power generation together with the power consumption of multiple building TCLs (such as heating, ventilation, and air conditioning (HVAC) systems, water heaters, and refrigerators) are performed. These analyses define the bandwidth over which these TCLs can operate and also describe the PV generation frequency bandwidth. Such spectral analyses, in frequency domain, can help identify the flexible components of PV generation that can be consumed by the various TCLs through optimal building load utilization. Second, a quadratic optimization problem based on model predictive control is formulated to allow consuming most of the low and medium frequency content of the PV power locally by building TCLs, while maintaining occupants’ comfort and TCLs’ physical constraints. The solution to the proposed optimization problem is achieved using optimal control strategies. Numerical results show that most of the low and medium frequency content of the PV generation can be consumed locally by building TCLs. The remaining high-frequency content of the PV generation can then be stored/offset using energy storage systems.

14 SOLAR ENERGY↗

An electromechanical actuation system for an expendable launch vehicle

A major effort at the NASA Lewis Research Center in recent years has been to develop electro-mechanical actuators (EMA's) to replace the hydraulic systems used for thrust vector control (TVC) on launch vehicles. This is an attempt ot overcome the inherent inefficiencies and costs associated with the existing hydraulic structures. General Dynamics Space Systems Division, under contract to NASA Lewis, is developing 18.6 kW (25 hp), 29.8 kW (40 hp), and 52.2 kW (70 hp) peak EMA systems to meet the power demands for TVC on a family of vehicles developed for the National Launch System. These systems utilize a pulse population modulated converter and field-oriented control scheme to obtain independent control of both the voltage and frequency. These techniques allow an induction motor to be operated at its maximum torque at all times. At NASA Lewis, we are building on this technology to develop our own in-house system capable of meeting the peak power requirements for an expendable launch vehicle (ELV) such as the Atlas. Our EMA will be capable of delivering 22.4 kW (30 hp) peak power with a nominal of 6.0 kW (8 hp). This system differs from the previous ones in two areas: (1) the use of advanced control methods, and (2) the incorporation of built-in-test. The advanced controls are essential for minimizing the controller size, while the built-in-test is necessary to enhance the system reliability and vehicle health monitoring. The ultimate goal of this program is to demonstrate an EMA which will be capable of self-test and easy integration into other projects. This paper will describe the effort underway at NASA Lewis to develop an EMA for an Atlas class ELV. An explanation will be given for each major technology block, and the status of each major technology block and the status of the overall program will be reported.

Burrows, Linda M.↗

Framework for Small-Scale Experiments in Software Engineering: Guidance and Control Software Project: Software Engineering Case Study

Software is becoming increasingly significant in today's critical avionics systems. To achieve safe, reliable software, government regulatory agencies such as the Federal Aviation Administration (FAA) and the Department of Defense mandate the use of certain software development methods. However, little scientific evidence exists to show a correlation between software development methods and product quality. Given this lack of evidence, a series of experiments has been conducted to understand why and how software fails. The Guidance and Control Software (GCS) project is the latest in this series. The GCS project is a case study of the Requirements and Technical Concepts for Aviation RTCA/DO-178B guidelines, Software Considerations in Airborne Systems and Equipment Certification. All civil transport airframe and equipment vendors are expected to comply with these guidelines in building systems to be certified by the FAA for use in commercial aircraft. For the case study, two implementations of a guidance and control application were developed to comply with the DO-178B guidelines for Level A (critical) software. The development included the requirements, design, coding, verification, configuration management, and quality assurance processes. This paper discusses the details of the GCS project and presents the results of the case study.

Hayhurst, Kelly J.↗

Computer graphics testbed to simulate and test vision systems for space applications

A system was developed for displaying computer graphics images of space objects and the use of the system was demonstrated as a testbed for evaluating vision systems for space applications. In order to evaluate vision systems, it is desirable to be able to control all factors involved in creating the images used for processing by the vision system. Considerable time and expense is involved in building accurate physical models of space objects. Also, precise location of the model relative to the viewer and accurate location of the light source require additional effort. As part of this project, graphics models of space objects such as the Solarmax satellite are created that the user can control the light direction and the relative position of the object and the viewer. The work is also aimed at providing control of hue, shading, noise and shadows for use in demonstrating and testing imaging processing techniques. The simulated camera data can provide XYZ coordinates, pitch, yaw, and roll for the models. A physical model is also being used to provide comparison of camera images with the graphics images.

Cheatham, John B.↗

Integrated Thermal Energy Storage System for Air-Conditioners with Phase-Change Composites

Thermal energy storage (TES) is a promising solution to store and dispatch energy and shave peak electric load, reducing the operational cost of HVAC systems. We present results of a TES system using phase-change materials (PCM) integrated with an air conditioner. The proposed system uses an organic PCM (tetradecane) encapsulated within compressed expanded natural graphite foams referred to as phase-change composite. The graphite foam encapsulates the PCM eliminating the need for expensive storage vessels, reduces installation costs, and provides higher thermal conductivity that can lead to faster charge/discharge rates. Two serpentines, multi-pass circuits, operating as a heat source and a sink, exchange heat to and from the phase-change composite. These two circuits are embedded in multiple slabs of this material. The "charge" circuit contains the refrigerant that is directly coupled to a vapor compression system, and the "discharge" circuit removes heat from an airstream and releases it into the PCM composite through a water-glycol liquid coupling. This configuration allows for multiple modes of operation depending on the state of charge of the thermal energy storage module, the building air-conditioning load, and the current electricity and demand charges. This flexible operation allows variable air volume capacity control without the need to have a variable capacity refrigeration system. We developed a 21 kW-hr (6 RT-hr) prototype TES system coupled with a commercial air-conditioner to characterize the component-and system-level performance.

air-conditioning↗

Intelligent multi-zone residential HVAC control strategy based on deep reinforcement learning

Residential heating, ventilation, and air conditioning (HVAC) has been considered as an important demand response resource. However, the optimization of residential HVAC control is no trivial task due to the complexity of the thermal dynamic models of buildings and uncertainty associated with both occupant-driven heat loads and weather forecasts. In this paper, we apply a novel model-free deep reinforcement learning (RL) method, known as the deep deterministic policy gradient (DDPG), to generate an optimal control strategy for a multi-zone residential HVAC system with the goal of minimizing energy consumption cost while maintaining the users’ comfort. Here, the applied deep RL-based method learns through continuous interaction with a simulated building environment and without referring to any prior model knowledge. Simulation results show that compared with the state-of-art deep Q network (DQN), the DDPG-based HVAC control strategy can reduce the energy consumption cost by 15% and reduce the comfort violation by 79%; and when compared with a rule-based HVAC control strategy, the comfort violation can be reduced by 98%. In addition, experiments with different building models and retail price models demonstrate that the well-trained DDPG-based HVAC control strategy has high generalization and adaptability to unseen environments, which indicates its practicability for real-world implementation.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Modeling Communication Infrastructures of Cyber Physical Systems

This effort explores modeling cyber infrastructure, including communication devices like switches, routers, sensors, and controllers, and physical media attributes like propagation of radio signals, in coordination with power distribution system model layouts. To do this, the project studies real-world configurations to define axioms of how different communication media is deployed with control equipment. These axioms will be used to develop tools to generate realistic cyber infrastructure models from starting power system models. This effort leverages and builds on the co-simulation platform developed by the other RD2C projects. The axioms and tools will be validated and demonstrated with the NS3 simulation tool as part of a co-simulation to show the behavior/impacts of cyber infrastructure on control operations.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Air Handling Unit Shutdowns During Scheduled Unoccupied Hours: US Commercial Building Stock Prevalence and Energy Impact

Commercial buildings account for 18% of U.S. energy consumption, with 44% used for heating, ventilation, and air conditioning (HVAC). American Society of Heating, Refrigerating and Air Conditioning Engineers (ASHRAE) 90.1 requires HVAC systems to shutdown fans and outdoor air ventilation during unoccupied times, only allowing fans to cycle on, without outdoor air, to maintain thermostat setpoints. However, it is minimally understood how often existing building operations align with energy code requirements and the energy implications of not doing so. This study used building automation system data from 843 buildings containing 5706 air handling units (AHUs) to determine three unoccupied AHU shutdown control schemes ranging in efficiency and then estimated their prevalence in the U.S. commercial building stock, segmented by building type. ComStock was then used to analyze the energy savings potential of implementing the most energy efficient unoccupied shutdown control scheme in non-participating buildings across the U.S commercial building stock. Results show that only 23% of AHUs align completely with the ASHRAE 90.1 requirement. ComStock modeling results show 4% annual stock energy savings by switching all non-participating buildings to the most efficient scheme, with 19% annual energy savings demonstrated for the median building switching from the least efficient scheme to the most efficient. Findings also show 114.5 TBtu electricity and 75.8 TBtu natural gas fuel savings when converting to the most efficient scheme. Furthermore, these findings help stakeholders understand the high prevalence of buildings not aligning with the ASHRAE-90.1 requirements for unoccupied AHU shutdowns and the energy savings potential of utilizing the most efficient unoccupied AHU shutdown scheme.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Integrated End-to-end Performance Prediction and Diagnosis for Extreme Scientific Workflows

This report details recent progress for the ASCR funded project “Integrated End-to-end Performance Prediction and Diagnosis for Extreme Scientific Workflows”. We refer to the project as IPPD/2, reflecting the 2017 renewal under expanded scope and partners In IPPD/2, we increased our research scope to include data motion. We are focusing on three major aspects: a) observe how data is generated, distributed, and used; b) analyze how data is (repeatedly) consumed with a focus both on repeated patterns and anomalies; and c) explore how to optimize data motion. This new work on data motion will augment and complement IPPD/2’s research that focused on the computational aspects of tasks. We leverage and extend our existing tools and demonstrate our work on the Belle II workflow suite as well as on workflows from NSLS-II. The highlights of our work are as follows: Provenance for Workflows: Provenance is used to provide information enabling quality control, re-run computational workflows, and reproduce results. IPPD/2 has been building a scalable provenance management system that enables the capture of provenance from the high-level workflow through all relevant system levels in one integrated environment. Leveraging this work, our recent efforts have included using provenance as an enabling technique. Workload characterization: Leveraging provenance and analysis, we characterize data movement within network, storage, and memory over a variety of workloads. This characterization enables an understanding by performance analysts and application developers of the range of behaviors that could be expected. Performance Prediction for Workflows: The goal of modeling distributed workflows is to understand performance bottlenecks and enable more intelligent task scheduling to optimize selected metrics of interest (e.g., task throughput or output data rate). IPPD/2 has utilized both analytical and AI/ML modeling methodologies for performance modeling. Advanced Scheduling and Fault Modeling for Workflows: Scheduling of large-scale scientific workflows on geographically distributed resources is a challenging problem. To improve workflow throughput, we combined novel scheduling algorithms with task predictions from performance modeling and fault modeling. Dynamically Alleviating Bottlenecks in Workflows: Exploiting our provenance, analysis, and modeling efforts, we have explored and developed several techniques for dynamically detecting and alleviating bottlenecks in data movement. In particular, we have spent considerable effort demonstrating our techniques on production-like workflow configurations.

97 MATHEMATICS AND COMPUTING↗

Koopman Model Predictive Control for Eco-Driving of Automated Vehicles

In this paper, we develop a data-driven process for building a model predictive control (MPC) for eco-driving of automated vehicles. The process involves performing system identification in which the non-linear vehicle dynamics model is approximated by the Koopman operator, a linear predictor of higher state-dimension, in a data-driven framework. This approach allows us to formulate the eco-driving problem in a constrained quadratic program that leads to a computationally fast MPC. The MPC is then implemented as a closed-loop control of an electric vehicle in numerical simulations for demonstration.

autonomous vehicle↗