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At least 631 records · Page 35

Environmental control medical support team

The activities conducted in support of the Environmental Control and Life Support Team during December 7, 1987 through September 30, 1988 are summarized. The majority of the ongoing support has focused on the ECLSS area. Through a series of initial meetings with the ECLSS team and technical literature review, an initial list of critical topics was developed. Subtasks were then identified or additional related tasks received as action items from the ECLSS group meetings. Although most of the efforts focused on providing MSFC personnel with information regarding specific questions and problems related to ECLSS issues, other efforts regarding identifying an ECLSS Medical Support Team and constructing data bases of technical information were also initiated and completed. The specific tasks are as follows: (1) Provide support to the mechanical design and integration of test systems as related to microbiological concerns; (2) Assist with design of Human Subjects Test Protocols; (3) Interpretation and recommendations pertaining to air/water quality requirements; (4) Assist in determining the design specifications required as related to the Technical Demonstration Program; (5) Develop a data base of all microorganisms recovered from previous subsystem testing; (6) Estimates of health risk of individual microbes to test subjects; (7) Assist with setting limits for safety of test subjects; (8) Health monitoring of test subjects; (9) Assist in the preparation of test plans; (10) Assist in the development of a QA/QC program to assure the validity, accuracy and precision of the analyses; and (11) Assist in developing test plans required for future man in the loop testing.

Crump, William J.↗

Microbial reduction of manganese oxides - Interactions with iron and sulfur

Alteromonas putrefaciens (strain MR-1) is capable of rapid Mn(IV) reduction under conditions of neutral pH and temperatures characteristic of the Oneida Lake, New York, sediments from which it was isolated. MR-1 also reduces Fe(3+) to Fe(2+), and disproportionates thiosulfate to sulfide and sulfite; independently, the Fe(2+) and sulfide act as rapid reductants of Mn. The addition of Fe(3+) or thiosulfate to cultures of MR-1 in the presence of oxidized Mn increases the rate and the extent of Mn reduction relative to that observed in the absence of Fe(3+) or thiosulfate. Furthermore, when Fe(3+) and Mn oxides are present conjointly, Fe(2+) does not appear until the reduction of the oxidized Mn is complete. These results demonstrate that the observed rates of Fe(2+) and sulfide production may underestimate the total rates of Fe and sulfate reduction in those environments containing oxidized Mn. These results also demonstrate the potential impact that a single microbe can exert on sediment geochemistry, and provide the basis for preliminary models of the complexity of microbial and geochemical interactions that occur.

Myers, Charles R.↗

Ecology of micro-organisms in a small closed system - Potential benefits and problems for Space Station

The probable sources and implications of microbial contamination on the proposed Space Station are discussed. Because of the limited availability of material, facilities and time on the Space Station, we are exploring the feasibility of replacing traditional incubation methods for assessing microbial contamination with rapid, automated methods. Some possibilities include: ATP measurement, microscopy and telecommunications, and molecular techniques such as DNA probes or monoclonal antibodies. Some of the important ecological factors that could alter microbes in space include microgravity, exposure to radiation, and antibiotic resistance.

Rodgers, E. B.↗

Microbial metabolism of Tholin

Tholin, a class of complex organic heteropolymers hypothesized to possess wide solar system distribution, is shown to furnish the carbon and energy requirements of a wide variety of common soil bacteria which encompasses aerobic, anaerobic, and facultatively anaerobic bacteria. Some of these bacteria are able to derive not merely their carbon but also their nitrogen requirements from tholin. The palatability of tholins to modern microbes is speculated to have implications for the early evolution of microbial life on earth; tholins may have formed the base of the food chain for an early heterotrophic biosphere, prior to the evolution of autotrophy on the early earth.

Stoker, C. R.↗

Source And Sink Of Iodine For Drinking Water

Proposed system for controlling concentration of iodine in potable water exploits temperature dependence of equilibrium partition of iodine between solution in water and residence in ion-exchange resin. Used to maintain concentration of iodine sufficient to kill harmful microbes, but not so great to make water unpalatable. Requires little attention, yet controls concentration of iodine more precisely than iodination and deiodination by manual techniques. Conceived for use aboard spacecraft, system has terrestrial applications in regions where water must be kept potable, resupply difficult, and system must operate largely unattended.

Sauer, Richard L.↗

Historical overview of the Biosphere 2 project

An extensive historical overview is given of the Biosphere 2 project. The overview begins in late 1969, as the moon landings commenced, when work began on ecological projects which laid the conceptual foundation for the current Biosphere 2 project. Continuing through to taking a complete functional suite of microbes together with their associated aquatic elements and an air volume and putting them inside a closed lab flask in which to measure the oxygen and CO2 levels, study energy flows and visually observe the changes therein. The laws of biospherics formulated by the author which can be tested in the Biosphere 2 project are listed.

Allen, John P.↗

Soil bed reactor work of the Environmental Research Lab. of the University of Arizona in support of the research and development of Biosphere 2

Research at the Environmental Research Lab in support of Biosphere 2 was both basic and applied in nature. One aspect of the applied research involved the use of biological reactors for the scrubbing of trace atmospheric organic contaminants. The research involved a quantitative study of the efficiency of operation of Soil Bed Reactors (SBR) and the optimal operating conditions for contaminant removal. The basic configuration of a SBR is that air is moved through a living soil that supports a population of plants. Upon exposure to the soil, contaminants are either passively adsorbed onto the surface of soil particles, chemically transformed in the soil to usable compounds that are taken up by the plants or microbes as a metabolic energy source and converted to CO2 and water.

Frye, Robert↗

The BioHome: A spinoff of space technology

The discussion of the BioHome is prefaced with some information about the work done at the environmental lab over the past 15 years concerning environmental issues related to biological life support such as the use of water hyacinths for wastewater purification, artificial marshes, indoor polluted air revitalization, and the reduction of organic contaminants using a biological system comprised of plants and microorganisms. One of the main concerns, especially with respect to a closed environment, is whether or not these systems are expelling microorganisms into the air. Analyses are being conducted to determine the numbers and types of microbes that are emitted. The BioHome is a 650 sq ft habitat that will enable the evaluation of the efficiency of bioregenerative technology in a closed system. This BioHome system is described and its functions discussed.

Johnson, Anne↗

Characterization of the corrosion resistance of several alloys to dilute biologically active solutions

Sulfate reducing bacteria and acid producing bacteria/fungi detected in hygiene waters increased the corrosion rate in aluminum alloy. Biologically active media enhanced the formation of pits on metal coupons. Direct observation of gas evolved at the corrosion sample, coupled with scanning electron microscopy (SEM) and energy dispersive x-ray analysis of the corrosion products indicates that the corrosion rate is increased because the presence of bacteria favor the reduction of hydrogen as the cathodic reaction through the reaction of oxygen and water. SEM verifies the presence of microbes in a biofilm on the surface of corroding samples. The bacterial consortia are associated with anodic sites on the metal surface, aggressive pitting occurs adjacent to biofilms. Many pits are associated with triple points and inclusions in the aluminum alloy microstructure. Similar bacterial colonization was found on the stainless steel samples. Fourier transform Infrared Spectroscopy confirmed the presence of carbonyl groups in pitted areas of samples exposed to biologically active waters.

Walsh, Daniel W.↗

Lunar base agriculture: Soils for plant growth

This work provides information on research and experimentation concerning various aspects of food production in space and particularly on the moon. Options for human settlement of the moon and Mars and strategies for a lunar base are discussed. The lunar environment, including the mineralogical and chemical properties of lunar regolith are investigated and chemical and physical considerations for a lunar-derived soil are considered. It is noted that biological considerations for such a soil include controlled-environment crop production, both hydroponic and lunar regolith-based; microorganisms and the growth of higher plants in lunar-derived soils; and the role of microbes to condition lunar regolith for plant cultivation. Current research in the controlled ecological life support system (CELSS) project is presented in detail and future research areas, such as the growth of higher research plants in CELSS are considered. Optimum plant and microbiological considerations for lunar derived soils are examined.

Ming, Douglas W.↗

Electrophoretic Process For Purifying Wastewater

Microbes, poisonous substances, and colloidal particles removed by combination of electric fields. Electrophoretic process removes pathogenicorganisms, toxins, toxic metals, and cooloidal soil particles from wastewater. Used to render domestic, industrial, and agricultural wastewater streams potable. Process also useful in bioregenerative and other closed systems like in space stations and submarines, where water must be recycled.

Sammons, David W.↗

Subsurface microbial habitats on Mars

We developed scenarios for shallow and deep subsurface cryptic niches for microbial life on Mars. Such habitats could have considerably prolonged the persistence of life on Mars as surface conditions became increasingly inhospitable. The scenarios rely on geothermal hot spots existing below the near or deep subsurface of Mars. Recent advances in the comparatively new field of deep subsurface microbiology have revealed previously unsuspected rich aerobic and anaerobic microbal communities far below the surface of the Earth. Such habitats, protected from the grim surface conditions on Mars, could receive warmth from below and maintain water in its liquid state. In addition, geothermally or volcanically reduced gases percolating from below through a microbiologically active zone could provide the reducing power needed for a closed or semi-closed microbial ecosystem to thrive.

Boston, P. J.↗

Planetary protection issues and human exploration of Mars

A key feature of the Space Exploration Initiative involves human missions to Mars. The report describing the initiative cites the search for life on Mars, extant or extinct, as one of the five science themes for such an endeavor. Because of this, concerns for planetary protection (PP) have arisen of two fronts: (1) forward contamination of Mars by spacecraft-borne terrestrial microbes which could interfere with exobiological analyses; and (2) back contamination of Earth by species that may be present in returned Mars samples. The United States is also signatory to an international treaty designed to protect Earth and planets from harmful cross-contamination during exploration. Therefore, it is timely to assess the necessity for, and impact of, PP procedures on the mission set comprising the human exploration of Mars. The ground-rules adopted at a recent workshop which addressed PP questions of this type are presented. In addition, the workshop produced several recommendations for dealing with forward and back contamination concerns for non-scientific perspectives, including public relations, legal, regulatory, international, and environmental.

Devincenzi, D. L.↗

The implantation of life on Mars - Feasibility and motivation

Scientific concepts are reviewed regarding the potential formation and development of a life-bearing environment on Mars, and a potential ecopoiesis scenario is given. The development of the earth's biosphere is defined, and the major assumptions related to the formation of Martian life are listed. Three basic phases are described for the life-implantation concept which include determining whether sufficient quantities of volatiles are available, engineering the warming of the planet, and implanting microbial communities if necessary. Warming the planet theoretically releases liquid H2O and produces a thick CO2 atmosphere, and the implantation of biological communities is only necessary if no indigenous microbes emerge. It is concluded that a feasibility study is required to assess the possibilities of implanting life on Mars more concretely.

Haynes, Robert H.↗

Evolution of a phase separated gravity independent bioreactor

The evolution of a phase-separated gravity-independent bioreactor is described. The initial prototype, a zero head-space manifold silicone membrane based reactor, maintained large diffusional resistances. Obtaining oxygen transfer rates needed to support carbon-recycling aerobic microbes is impossible if large resistances are maintained. Next generation designs (Mark I and II) mimic heat exchanger design to promote turbulence at the tubing-liquid interface, thereby reducing liquid and gas side diffusional resistances. While oxygen transfer rates increased by a factor of ten, liquid channeling prevented further increases. To overcome these problems, a Mark III reactor was developed which maintains inverted phases, i.e., media flows inside the silicone tubing, oxygen gas is applied external to the tubing. This enhances design through changes in gas side driving force concentration and liquid side turbulence levels. Combining an applied external pressure of 4 atm with increased Reynolds numbers resulted in oxygen transfer intensities of 232 mmol O2/l per hr (1000 times greater than the first prototype and comparable to a conventional fermenter). A 1.0 liter Mark III reactor can potentially deliver oxygen supplies necessary to support cell cultures needed to recycle a 10-astronaut carbon load continuously.

Villeneuve, Peter E.↗

Microbial growth and physiology in space - A review

An overview of microbial behavior in closed environments is given with attention to data related to simulated microgravity and actual space flight. Microbes are described in terms of antibiotic sensitivity, subcellular structure, and physiology, and the combined effects are considered of weightlessness and cosmic radiation on human immunity to such microorganisms. Space flight results report such effects as increased phage induction, accelerated microbial growth rates, and the increased risk of disease communication and microbial exchange aboard confining spacecraft. Ultrastructural changes are also noted in the nuclei, cell membranes, and cytoplasmic streaming, and it appears that antibiotic sensitivity is reduced under both actual and simulated conditions of spaceflight.

Cioletti, Louis A.↗

Bioburden control for Space Station Freedom's Ultrapure Water System

Bioburden control is one of the challenges for the Ultrapure Water System on Space Station Freedom. Bioburden control must enable the system to deliver water with a low bacterial count as well as maintain biological contamination at a manageable level, to permit continued production of quality water. Ozone has been chosen as the primary means of Bioburden control. Planned tests to determine the effectiveness of ozone on free-floating microbes and biofilms are described.

Snodgrass, Donald W.↗

Lunar outpost agriculture

The development of a CELSS for a lunar outpost is discussed. It is estimated that a lunar outpost life support system with a crew of four that produces food would break even in terms of mass and cost to deliver the system to the lunar surface after 2.5 years when compared to the cost of resupply from earth. A brief review is made of research on life support systems and NASA projects for evaluating CELSS components. The use of on-site materials for propellants, construction materials, and agriculture is evaluated, and the use of microbes for waste decomposition and stabilization of ecological balance is touched upon. Areas for further investigation include the behavior of organisms in microgravity, genetic alteration, gas exchange capabilities of organisms, integration of biological and physicochemical components, and automation. The development stages leading to lunar deployment are outlined.

Hossner, Lloyd R.↗