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At least 649 records · Page 36

Bone and Cartilage Degeneration in Mice Following Long-Duration Spaceflight: The Role of Bone Marrow Stem Cells

The detrimental effects of mechanical unloading in microgravity, including the musculo-skeletal system, are well documented. However, the effects of mechanical unloading on joint health and the interaction between bone and cartilage specifically, are less well known. Our ongoing studies with the mouse bone model have identified the failure of normal stem cell-based tissue regeneration, in addition to tissue degeneration, as a significant concern for long-duration spaceflight, especially in the mesenchymal and hematopoietic tissue lineages. Furthermore, we have identified the cell cycle arrest molecule, CDKN1ap21, as specifically up-regulated during spaceflight exposure and localized to osteoprecursors on the bone surface and chondroprogenitors in articular cartilage that are both required for normal tissue regeneration. The 30-day BionM1 and 37-day Rodent Research 1 (RR1) missions enabled the possibility of studying these effects in long-duration microgravity experiments. We hypothesized that the inhibition of stem cell-based tissue regeneration in short-duration spaceflight would continue during long-duration spaceflight resulting in significant tissue alterations and we specifically studied the hip joint (pelvis and proximal femur) to elucidate these effects. To test this hypothesis we analyzed bone and bone marrow stem cells using techniques including high-resolution Microcomputed Tomography (MicroCT), in-vivo differentiation and migration assays, and whole transcriptome expression profiling. We found that exposure to spaceflight for 30 days results in a significant decrease in bone volume fraction (-31), trabecular thickness (-14) and trabecular number (-20). Similar decrements in bone volume fraction (-27), trabecular number (-13) and trabecular thickness (-17) were found in female mice exposed to 37 days spaceflight. Furthermore, high-resolution MicroCT and immunohistochemical analysis of spaceflight tissues revealed a severe disruption of the epiphyseal boundary, resulting in endochondral ossification of the femoral head and perforation of articular cartilage by bone. This suggests that spaceflight in microgravity may cause rapid induction of an aging-like phenotype with signs of osteoarthritic disease in the hip joint. Microarray analysis also revealed that the top pathways altered during spaceflight include activation of matrix metalloproteinases, oxidative stress signaling and inflammation in both whole bone tissue and isolated bone marrow stem cells. In conclusion, the observed inhibition of stem cell-based tissue regeneration persists during long-duration spaceflight. Furthermore, spaceflight mice exhibit disruption of the epiphyseal boundary and endochondral ossification of the femoral head, and an inhibition of stem cell based tissue regeneration, which, taken together, may indicate onset of an accelerated aging phenotype with signs of osteoarthritic disease.

Cartilage↗

Evaluation of Cation Migration in Lanthanum Strontium Cobalt Ferrite Solid Oxide Fuel Cell Cathodes via In-Operando X-ray Diffraction

Anode-supported SOFC button cells with LSCF-6428 cathodes were operated at various temperatures under constant current conditions for hundreds of hours in dry or humid (~3% water) cathode air while continuously collecting 1 hour XRD scans throughout the test duration. Additionally, one cell in dry air was held at OCV, and 12% CO2 was added to the humid air for another cell. Long cumulative XRD count times allowed minor phases in concentrations of less than 0.1 wt% to be identified. In humid air, cell performance increased during the first couple of hundred hours. XRD measured a gradual shift of Fe-rich Fe,Co spinel peaks to lower angles while post-mortem SEM discovered nano-nodules on the LSCF surfaces. When 12% CO2 was added to humid air, performance degradation was observed after the initial conditioning response, unlike in tests without CO2, which were stable after conditioning. XRD also measured a shift in the peaks of the major LSCF phase in the test with CO2 indicative of some degree of gradual decomposition. Cells tested at constant current in dry air exhibited fast initial degradation that decelerated over time, while the cell held at OCV maintained a stable voltage. XRD of the cathodes held at OCV at 750 °C and operated at constant current at 700 °C in dry air measured a slower shift of the Fe-rich spinel peaks to lower angles than was seen in tests in humid air, along with a shift in the peaks of a Co-rich Fe,Co spinel to higher angles. Post-mortem SEM of cells exposed to dry air discovered smaller nano-nodules on the LSCF surfaces than had been present in cells tested in humid air. Cells tested at constant current in dry air at 750 °C and 800 °C did not exhibit any gradual changes in the XRD patterns nor were nano-nodules discovered.

Hardy, John S.↗

Soldering Tested in Reduced Gravity

Whether used occasionally for contingency repair or routinely in nominal repair operations, soldering will become increasingly important to the success of future long-duration human space missions. As a result, it will be critical to have a thorough understanding of the service characteristics of solder joints produced in reduced-gravity environments. The National Center for Space Exploration Research (via the Research for Design program), the NASA Glenn Research Center, and the NASA Johnson Space Center are conducting an experimental program to explore the influence of reduced gravity environments on the soldering process. Solder joint characteristics that are being considered include solder fillet geometry, porosity, and microstructural features. Both through-hole (see the drawing and image on the preceding figure) and surface-mounted devices are being investigated. This effort (the low-gravity portion being conducted on NASA s KC-135 research aircraft) uses the soldering hardware currently available on the International Space Station. The experiment involves manual soldering by a contingent of test operators, including both highly skilled technicians and less skilled individuals to provide a skill mix that might be encountered in space mission crews. The experiment uses both flux-cored solder and solid-core solder with an externally applied flux. Other experimental parameters include the type of flux, gravitational level (nominally zero,

Struk, Peter M.↗

A technology data base for the design of 500 to 5000-lb thrust class liquid rocket engines utilizing hydrogen and oxygen as propellants

This paper presents an overview of the results of experimental evaluations of candidate designs for igniters, injectors, and propellant-cooled thrust chambers applicable to restartable high-performance, high-reliability upper-stage engines and to pulsing-type reaction control engines (RCE). Injection element types best suited for liquid, gas, and liquid/gas phase propellant supply are identified. The resulting interactions between element type, combustion efficiency, and chamber wall heating are compared. The distinction between thrust chamber design requirements for upper stage vs RCE applications as measured by cycle life requirements is translated into design configurations consisting of all-film-cooled, all-regeneratively-cooled, and composites of the two cooling approaches. The validity of the design approaches is confirmed by data from engine durability testing involving over 90,000 starts and 9,000 thermal cycles on RCE-type designs and multiple long-duration burns (up to 2,000 sec) on regeneratively cooled upper-stage designs.

Schoenman, L.↗

High-Pressure Oxygen Generation for Outpost EVA

Low Lunar Orbit (LLO) poses unique thermal challenges for the orbiting space craft, particularly regarding the performance of the radiators. The emitted infrared (IR) heat flux from the lunar surface varies drastically from the light side to the dark side of the moon. Due to the extremely high incident IR flux, especially at low beta angles, a radiator is oftentimes unable to reject the vehicle heat load throughout the entire lunar orbit. One solution to this problem is to implement Phase Change Material (PCM) Heat Exchangers. PCM Heat Exchangers act as a "thermal capacitor, storing thermal energy when the radiator is unable to reject the required heat load. The stored energy is then removed from the PCM heat exchanger when the environment is more benign. Because they do not use an expendable resource, such as the feed water used by sublimators and evaporators, PCM Heat Exchangers are ideal for long duration Low Lunar Orbit missions. The Advanced Thermal Control project at JSC is completing a PCM heat exchanger life test to determine whether further technology development is warranted. The life test is being conducted on four nPentadecane, carbon filament heat exchangers. Fluid loop performance, repeatability, and measurement of performance degradation over 2500 meltfreeze cycles will be performed and reported in the current document.

Jeng, Frank↗

NASA Exercise Physiology and Countermeasures Project Overview

Efficient exercise countermeasures are necessary to offset or minimize spaceflight-induced deconditioning and to maximize crew performance of mission tasks. These countermeasure protocols should use the fewest crew and vehicle resources. NASA s Exercise Physiology and Countermeasures (ExPC) Project works to identify, collect, interpret, and summarize evidence that results in effective exercise countermeasure protocols which protect crew health and performance during International Space Station (ISS) and future exploration-class missions. The ExPC and NASA s Human Research Program are sponsoring multiple studies to evaluate and improve the efficacy of spaceflight exercise countermeasures. First, the Project will measure maximal aerobic capacity (VO2max) during cycle ergometry before, during, and after ISS missions. Second, the Project is sponsoring an evaluation of a new prototype harness that offers improved comfort and increased loading during treadmill operations. Third, the Functional Tasks Test protocol will map performance of anticipated lunar mission tasks with physiologic systems before and after short and long-duration spaceflight, to target system contributions and the tailoring of exercise protocols to maximize performance. In addition to these studies that are actively enrolling crewmember participants, the ExPC is planning new studies that include an evaluation of a higher-intensity/lower-volume exercise countermeasure protocol aboard the ISS using the Advanced Resistive Exercise Device and second-generation treadmill, studies that evaluate bone loading during spaceflight exercise, and ground-based studies that focus on fitness for duty standards required to complete lunar mission tasks and for which exercise protocols need to protect. Summaries of these current and future studies and strategies will be provided to international colleagues for knowledge sharing and possible collaboration.

Loerch, Linda↗

Large Scale Experiments on Spacecraft Fire Safety

Full scale fire testing complemented by computer modelling has provided significant know how about the risk, prevention and suppression of fire in terrestrial systems (cars, ships, planes, buildings, mines, and tunnels). In comparison, no such testing has been carried out for manned spacecraft due to the complexity, cost and risk associated with operating a long duration fire safety experiment of a relevant size in microgravity. Therefore, there is currently a gap in knowledge of fire behaviour in spacecraft. The entire body of low-gravity fire research has either been conducted in short duration ground-based microgravity facilities or has been limited to very small fuel samples. Still, the work conducted to date has shown that fire behaviour in low-gravity is very different from that in normal-gravity, with differences observed for flammability limits, ignition delay, flame spread behaviour, flame colour and flame structure. As a result, the prediction of the behaviour of fires in reduced gravity is at present not validated. To address this gap in knowledge, a collaborative international project, Spacecraft Fire Safety, has been established with its cornerstone being the development of an experiment (Fire Safety 1) to be conducted on an ISS resupply vehicle, such as the Automated Transfer Vehicle (ATV) or Orbital Cygnus after it leaves the ISS and before it enters the atmosphere. A computer modelling effort will complement the experimental effort. Although the experiment will need to meet rigorous safety requirements to ensure the carrier vehicle does not sustain damage, the absence of a crew removes the need for strict containment of combustion products. This will facilitate the possibility of examining fire behaviour on a scale that is relevant to spacecraft fire safety and will provide unique data for fire model validation. This unprecedented opportunity will expand the understanding of the fundamentals of fire behaviour in spacecraft. The experiment is being developed by an international topical team that is collaboratively defining the experiment requirements and performing supporting analysis, experimentation and technology development. This paper presents the objectives, status and concept of this project.

Urban, David L.↗

Large Scale Experiments on Spacecraft Fire Safety

Full scale fire testing complemented by computer modelling has provided significant knowhow about the risk, prevention and suppression of fire in terrestrial systems (cars, ships, planes, buildings, mines, and tunnels). In comparison, no such testing has been carried out for manned spacecraft due to the complexity, cost and risk associated with operating a long duration fire safety experiment of a relevant size in microgravity. Therefore, there is currently a gap in knowledge of fire behaviour in spacecraft. The entire body of low-gravity fire research has either been conducted in short duration ground-based microgravity facilities or has been limited to very small fuel samples. Still, the work conducted to date has shown that fire behaviour in low-gravity is very different from that in normal gravity, with differences observed for flammability limits, ignition delay, flame spread behaviour, flame colour and flame structure. As a result, the prediction of the behaviour of fires in reduced gravity is at present not validated. To address this gap in knowledge, a collaborative international project, Spacecraft Fire Safety, has been established with its cornerstone being the development of an experiment (Fire Safety 1) to be conducted on an ISS resupply vehicle, such as the Automated Transfer Vehicle (ATV) or Orbital Cygnus after it leaves the ISS and before it enters the atmosphere. A computer modelling effort will complement the experimental effort. Although the experiment will need to meet rigorous safety requirements to ensure the carrier vehicle does not sustain damage, the absence of a crew removes the need for strict containment of combustion products. This will facilitate the possibility of examining fire behaviour on a scale that is relevant to spacecraft fire safety and will provide unique data for fire model validation. This unprecedented opportunity will expand the understanding of the fundamentals of fire behaviour in spacecraft. The experiment is being developed by an international topical team that is collaboratively defining the experiment requirements and performing supporting analysis, experimentation and technology development. This paper presents the objectives, status and concept of this project.

Urban, David↗

Testing of a Helium Loop Heat Pipe for Large Area Cryocooling

Future NASA space telescopes and exploration missions require cryocooling of large areas such as optics, detector arrays, and cryogenic propellant tanks. One device that can potentially be used to provide closed-loop cryocooling is the cryogenic loop heat pipe (CLHP). A CLHP has many advantages over other devices in terms of reduced mass, reduced vibration, high reliability, and long life. A helium CLHP has been tested extensively in a thermal vacuum chamber using a cryocooler as the heat sink to characterize its transient and steady performance and verify its ability to cool large areas or components in the 3K temperature range. A copper plate with attached electrical heters was used to simulate the heat source, and heat was collected by the CLHP evaporator and transferred to the cryocooler for ultimate heat rejection. The helium CLHP thermal performance test included cool-down from the ambient temperature, startup, capillary limit, heat removal capability, rapid power changes, and long duration steady state operation. The helium CLHP demonstrated robust operation under steady state and transient conditions. The loop could be cooled from the ambient temperature to subcritical temperatures very effectively, and could start successfully without pre-conditioning by simply applying power to both the capillary pump and the evaporator plate. It could adapt to rapid changes in the heat load, and reach a new steady state very quickly. Heat removal between 10mW and 140mW was demonstrated, yielding a power turn down ratio of 14. When the CLHP capillary limit was exceeded, the loop could resume its normal function by reducing the power to the capillary pump. Steady state operations up to 17 hours at several heat loads were demonstrated. The ability of the helium CLHP to cool large areas was therefore successfully verified.

Crycooling↗

Testing of a Helium Loop Heat Pipe for Large Area Cryocooling

Future NASA space telescopes and exploration missions require cryocooling of large areas such as optics, detector arrays, and cryogenic propellant tanks. One device that can potentially be used to provide closed-loop cryocooling is the cryogenic loop heat pipe (CLHP). A CLHP has many advantages over other devices in terms of reduced mass, reduced vibration, high reliability, and long life. A helium CLHP has been tested extensively in a thermal vacuum chamber using a cryocooler as the heat sink to characterize its transient and steady performance and verify its ability to cool large areas or components in the 3K temperature range. A copper plate with attached electrical heaters was used to simulate the heat source, and heat was collected by the CLHP evaporator and transferred to the cryocooler for ultimate heat rejection. The helium CLHP thermal performance test included cool-down from the ambient temperature, startup, capillary limit, heat removal capability, rapid power changes, and long duration steady state operation. The helium CLHP demonstrated robust operation under steady state and transient conditions. The loop could be cooled from the ambient temperature to subcritical temperatures very effectively, and could start successfully without pre-conditioning by simply applying power to both the capillary pump and the evaporator plate. It could adapt to rapid changes in the heat load, and reach a new steady state very quickly. Heat removal between 10mW and 140mW was demonstrated, yielding a power turn down ratio of 14. When the CLHP capillary limit was exceeded, the loop could resume its normal function by reducing the power to the capillary pump. Steady state operations up to 17 hours at several heat loads were demonstrated. The ability of the helium CLHP to cool large areas was therefore successfully verified.

cryocooling↗

Lunar Health Monitor (LHM)

Orbital Research, Inc., has developed a low-profile, wearable sensor suite for monitoring astronaut health in both intravehicular and extravehicular activities. The Lunar Health Monitor measures respiration, body temperature, electrocardiogram (EKG) heart rate, and other cardiac functions. Orbital Research's dry recording electrode is central to the innovation and can be incorporated into garments, eliminating the need for conductive pastes, adhesives, or gels. The patented dry recording electrode has been approved by the U.S. Food and Drug Administration. The LHM is easily worn under flight gear or with civilian clothing, making the system completely versatile for applications where continuous physiological monitoring is needed. During Phase II, Orbital Research developed a second-generation LHM that allows sensor customization for specific monitoring applications and anatomical constraints. Evaluations included graded exercise tests, lunar mission task simulations, functional battery tests, and resting measures. The LHM represents the successful integration of sensors into a wearable platform to capture long-duration and ambulatory physiological markers.

Lisy, Frederick J.↗

Thermal Vacuum Testing of a Helium Loop Heat Pipe for Large Area Cryocooling

Future NASA space telescopes and exploration missions require cryocooling of large areas such as optics, detector arrays, and cryogenic propellant tanks. One device that can potentially be used to provide closed-loop cryocooling is the cryogenic loop heat pipe (CLHP). A CLHP has many advantages over other devices in terms of reduced mass, reduced vibration, high reliability, and long life. A helium CLHP has been tested extensively in a thermal vacuum chamber using a cryocooler as the heat sink to characterize its transient and steady performance and to verify its ability to cool large areas or components in the 3 degrees Kelvin temperature range. The helium CLHP thermal performance test included cool-down from the ambient temperature, startup, capillary limit, heat removal capability, rapid power changes, and long duration steady state operation. The helium CLHP demonstrated robust operation under steady state and transient conditions. The loop could be cooled from the ambient temperature to subcritical temperatures very effectively, and could start successfully by simply applying power to both the capillary pump and the evaporator plate without pre-conditioning. It could adapt to a rapid heat load change and quickly reach a new steady state. Heat removal between 10 megawatts and 140 megawatts was demonstrated, yielding a power turn down ratio of 14. When the CLHP capillary limit was exceeded, the loop could resume its normal function by reducing the power to the capillary pump. Steady state operations up to 17 hours at several heat loads were demonstrated. The ability of the helium CLHP to cool large areas was therefore successfully verified.

Thermal Control Systems↗

Researching Plant Growth in Amended Martian Regolith Simulant, Photosynthetic Rates of Plants, Seed Surface Decontamination by Plasma Methods, New Crop Development, and Porous Concrete Media

Plant growth research for food production at Kennedy Space Center looks at how future residents of Mars and the Moon will enjoy the sight, smell, taste, and nutrition of plants. Overall, the goal is to provide a sustainable source of healthy food, on long-duration space flights, so astronauts can get the nutrition they need and produce food. The sustainable production of food will aid in the efforts of closed life support. Plants have a vital application for bio regenerative life support as demands for food and oxygen can be provided through photosynthesis, while the carbon dioxide from human respiration is removed. Transpiration is also used in life support processes as waste water that can be recycled through plant systems with the resultant humidity then condensed as clean water. Selected crops will provide the nutrient requirements needed for long duration space flight. Currently, projects in food production are investigating how plants grow in Martian regolith simulant, new crops testing with tomato and pepper cultivars, acquiring real-time photosynthetic data on crops, assessing plant growth in porous concrete media, and the use of plasma for surface decontamination of seeds.

food production↗

Regulatory Approach for Nuclear Thermal Propulsion Reactor Systems

Nuclear Thermal Propulsion (NTP) is being developed to support crewed or cargo transfer missions to Mars. Obtaining regulatory approval for NTP engine testing proves a challenge for not only the technology maturation required but also the fabrication, launch and operational nuclear regulatory environments. Existing regulations currently apply to either (1) high power, long duration commercial power plants, or (2) low power, short duration research reactors. NTP systems find themselves in a separate and unique area due to their higher power but short operating duration. This is supplemented by use of the reactor coolant as propellant. When planning the regulatory approach for NTP systems, operations in space and testing on Earth must be considered – each have their own challenges. This paper identifies a preliminary pathway for regulatory approval to operate a NTP demonstration engine as well as supporting test data that is recommended to be generated during the development program to support major regulatory milestones and deliverables.

Regulatory↗

Regulatory Approach for Nuclear Thermal Propulsion

Nuclear Thermal Propulsion (NTP) is being developed to support crewed or cargo transfer missions to Mars. Obtaining regulatory approval for NTP engine testing proves a challenge for not only the technology maturation required but also the fabrication, launch and operational nuclear regulatory environments. Existing regulations currently apply to either (1) high power, long duration commercial power plants, or (2) low power, short duration research reactors. NTP systems find themselves in a separate and unique area due to their higher power but short operating duration. This is supplemented by use of the reactor coolant as propellant. When planning the regulatory approach for NTP systems, operations in space and testing on Earth must be considered – each have their own challenges. This paper identifies a preliminary pathway for regulatory approval to operate a NTP demonstration engine as well as supporting test data that is recommended to be generated during the development program to support major regulatory milestones and deliverables.

Regulatory↗

EUSO-SPB2 Fluorescence Telescope trigger test within the EUSO@TurLab Project

The second generation Extreme Universe Space Observatory on a Super-Pressure Balloon (EUSO-SPB2) mission is on track to be approved by NASA for a long duration flight (up to 100 days) in 2023 from Wanaka, New Zealand. EUSO-SPB2 will monitor the night atmosphere of the Southern hemisphere from 33 km altitude to detect Ultra-High Energy Cosmic Rays (UHECRs) and search for astrophysical and cosmogenic neutrinos. EUSO-SPB2 features two telescopes, a Cherenkov (CT) and a Fluorescence (FT) Telescope. The hardware implementation and performance of the FT trigger logic were tested at the TurLab facility, hosted at the University of Turin. In conclusion, the tests proved the correct hardware implementation of the trigger logic and its ability to detect μs timescale transient signals.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Solar Absorptivity Degradation of Spacecraft Materials Due to UV and Charged Particles in the Gateway Environment

Traditionally, most of the data accumulated for material optical property degradation in space has been in the Low Earth Orbit (LEO) environment. However, Gateway is a new space station that will be assembled and flown in Near Rectilinear Halo Orbit (NRHO) for 15 years. Additionally, some Gateway modules will have a slow transit from Earth to NRHO that will take over a year to pass through the Van Allen Belts, which contain damaging charged particles. The environments Gateway modules will encounter are not well characterized. Environmental factors such as charged particles and ultraviolet (UV) radiation have been shown to significantly increase solar absorptivity (α) for some thermal control materials and coatings. Since α will impact thermal performance over the mission, the Gateway Passive Thermal Control System (PTCS) Group has identified charged particle and UV degradation of Gateway materials as a knowledge gap. Candidate materials and coatings were subjected to expected mission charged particle environments and over 5000 Equivalent Solar Hours (ESH) of UV at NASA Goddard Space Flight Center (GSFC). In situ change in reflectivity was observed at specified intervals during testing such that change in α could be plotted as a function of time of solar exposure.

Optical Property↗